Terese Soudah

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Muhammad Abdul Hadi

De Montfort University

Debes Ray

Bhabha Atomic Research Centre

Puck Suwannaprom

Chiang Mai University

Moin Basha

JNTU , hyderabad

Md Kamal Hossain

Jahangirnagar University

Niki Bardi

National Centre of Scientific Research "DEMOKRITOS"

World Journal of Pharmaceutical Sciences

Daniel Reim

Technical University of Munich

Antonino Battaglia

Università degli Studi di Torino

Yoany Garcia

Florida International University

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Papers by Terese Soudah

Desorption electrospray ionization mass spectrometry imaging in discovery and development of novel therapies

Mass spectrometry reviews, 2021

Desorption electrospray ionization mass spectrometry imaging (DESI-MSI) is one of the least speci... more Desorption electrospray ionization mass spectrometry imaging (DESI-MSI) is one of the least specimen destructive ambient ionization mass spectrometry tissue imaging methods. It enables rapid simultaneous mapping, measurement, and identification of hundreds of molecules from an unmodified tissue sample. Over the years, since its first introduction as an imaging technique in 2005, DESI-MSI has been extensively developed as a tool for separating tissue regions of various histopathologic classes for diagnostic applications. Recently, DESI-MSI has also emerged as a versatile technique that enables drug discovery and can guide the efficient development of drug delivery systems. For example, it has been increasingly employed for uncovering unique patterns of in vivo drug distribution, the discovery of potentially treatable biochemical pathways, revealing novel druggable targets, predicting therapeutic sensitivity of diseased tissues, and identifying early tissue response to pharmacological...

AntimiR-155 Cyclic Peptide–PNA Conjugate: Synthesis, Cellular Uptake, and Biological Activity

ACS Omega

Efficient delivery of nucleic acids into cells still remains a great challenge. Peptide nucleic a... more Efficient delivery of nucleic acids into cells still remains a great challenge. Peptide nucleic acids (PNAs) are DNA analogues with a neutral backbone and are synthesized by solid phase peptide chemistry. This allows a straightforward synthetic route to introduce a linear short peptide (a.k.a. cellpenetrating peptide) to the PNA molecule as a means of facilitating cellular uptake of PNAs. Herein, we have devised a synthetic route in which a cyclic peptide is prepared on a solid support and is extended with the PNA molecule, where all syntheses are accomplished on the solid phase. This allows the conjugation of the cyclic peptide to the PNA molecule with the need of only one purification step after the cyclic peptide− PNA conjugate (C 9 −PNA) is cleaved from the solid support. The PNA sequence chosen is an antimiR-155 molecule that is complementary to mature miR-155, a well-established oncogenic miRNA. By labeling C 9 −PNA with fluorescein isothiocyanate, we observe efficient cellular uptake into glioblastoma cells (U87MG) at a low concentration (0.5 μM), as corroborated by fluorescence-activated cell sorting (FACS) analysis and confocal microscopy. FACS analysis also suggests an uptake mechanism that is energy-dependent. Finally, the antimiR activity of C 9 −PNA was shown by analyzing miR155 levels by quantitative reverse transcription polymerase chain reaction and by observing a reduction in cell viability and proliferation in U87MG cells, as corroborated by XTT and colony formation assays. Given the added biological stability of cyclic versus linear peptides, this synthetic approach may be a useful and straightforward approach to synthesize cyclic peptide−PNA conjugates.

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Process for the Preparation of a Stable Polymorphic Form of Atovaquone

Process for the Preparation of a Stable Polymorphic Form of Atovaguone

CLIP6-PNA-Peptide Conjugates: Non-Endosomal Delivery of Splice Switching Oligonucleotides

Bioconjugate Chemistry