A 70-year-old woman with rheumatoid arthritis (RA) and secondary amyloidosis presented repeated c... more A 70-year-old woman with rheumatoid arthritis (RA) and secondary amyloidosis presented repeated consciousness loss. The pathological findings at autopsy revealed multi-organic deposits of amyloid A-protein and so-called 'plexiform change' of blood vessels in bilateral temporal lobes. The arterial plexiform change, which is found in the lung specimen of primary pulmonary hypertension, might be a new pathological cerebrovascular change associated with RA.
Although the roles of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF... more Although the roles of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) in angiogenesis are well described , the putative roles of these factors in retinopathy of prematurity (ROP) remain unknown. We evaluated VEGF and HGF protein levels in subretinal fluid of eyes with ROP , and expression of their corresponding receptors in retrolental membranes associated with stage 5 ROP. We examined subretinal fluid samples from eyes using rhegmatogenous retinal detachment as a control. VEGF and HGF were differentially elevated in eyes with ROP. In Stage 5 ROP (n ؍ 22) , the mean VEGF and HGF levels were 14.77 ؎ 14.01 ng/ml and 16.56 ؎ 9.62 ng/ml , respectively. Interestingly , in patients with active stage 4 ROP , mean VEGF levels were highly elevated (44.16 ؎ 18.72 ng/ml) , whereas mean HGF levels remained very low (4.77 ؎ 2.50 ng/ml). Next, we investigated in vivo expression of VEGF receptor-2 and HGF receptor in retrolental membranes from 16 patients with stage 5 ROP. Both VEGF receptor-2 and HGF receptor proteins were detected mainly in posterior portions of the membrane as well as in vessel walls and along the retinal interface where angiogenesis was active. These findings together suggest that VEGF and HGF play important roles in the pathogenesis of ROP.
Stargardt disease is the most common form of autosomal recessive macular dystrophy. Mutation in t... more Stargardt disease is the most common form of autosomal recessive macular dystrophy. Mutation in the ABCA4 gene (ABCR protein) is responsible for disease manifestation in more than 95% of Stargardt patients. ABCA4 codes for a member of the ATP binding cassette transmembrane protein involved in the transport of all-trans retinal. Dysfunction in this protein causes accumulation of lipofuscin, which is toxic to the RPE and photoreceptors. Presenting symptoms, fundus appearance, and progression of the disease are widely variable in this disease. Different imaging modalities have been utilized to study the presentation and evolution of fundus changes. Although there is ongoing research to better understand the disease process and ways to alternate its path, currently there is no treatment for Startgardt patients.
The advent of optical coherence tomography (OCT) technology has greatly enhanced our understandin... more The advent of optical coherence tomography (OCT) technology has greatly enhanced our understanding of vitreoretinal diseases; it has become a routine diagnostic imaging method for the evaluation of vitreoretinal abnormalities and injuries in adult and pediatric patients. The use of OCT has recently been extended beyond the offices and clinics to perioperative and intraoperative settings. The new development in high-resolution and high-speed spectral domain OCT, along with the improvement in portability, has made the device more valuable than ever before. Our article summarizes the current development of the portable OCT devices in clinical, perioperative, and intraoperative settings in the management of vitreoretinal diseases.
In order to clarify the role of CD5 antigen on B cell in autoimmunity, we examined B cells from p... more In order to clarify the role of CD5 antigen on B cell in autoimmunity, we examined B cells from patients with rheumatoid arthritis (RA). The percentages of CD5 positive B cells were increased in peripheral blood from RA compared with normal. Normal and RA B cells were stimulated with two kinds of monoclonal antibodies to CD5 (Leu-1, SL-1) which recognize different epitopes. RA B cells proliferated and secreted IgM by CD5 antibody stimulation in combination with IL-1. Our observations imply that CD5 positive B cells in RA are in their differentiation stage and that CD5 antigen might be one of the triggers to activate CD5 positive B cells in vivo to produce autoantibody.
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1990
The uptake of LDL and acetylated LDL and the ability of cholesteryl ester accumulation by cells o... more The uptake of LDL and acetylated LDL and the ability of cholesteryl ester accumulation by cells of a human monocytic cell line, U937, has been characterized by flow cytometric assay using a fluorescent probe, DiI, and by high-performance liquid chromatography (HPLC). The increase of mean fluorescence intensity of U937 incubated with DiI-labeled lipoproteins demonstrates that this cell line could incorporate DiI-AcLDL, as well as DiI-labeled LDL. Competition and saturation studies indicate that the manner of taking up DiI-AcLDL is receptor-mediated. While differentiated U937 incubated with 16 nM phorbol myristate acetate for 24 h took up little DiI-AcLDL, HPLC analysis confirmed that intracellular free and esterified cholesterols significantly increase in the U937 cells incubated with AcLDL or LDL. The ability of mouse peritoneal macrophage to abundantly accumulate at least five kinds of cholesteryl ester were also shown in this analysis. In contrast, in U937 cells, free fatty acids are incorporated into various substances rather than into cholesteryl esters (as revealed by HPLC analysis), so that the cholesterol in AcLDL taken up by U937 cells is not synthesized into cholesteryl esters to any great extent.
While angiogenesis inhibitors are already widely used to treat retinal disease in adults, only li... more While angiogenesis inhibitors are already widely used to treat retinal disease in adults, only limited reports are currently available for the use of anti-VEGF in pediatric vitreoretinal diseases such as retinopathy of prematurity, Coats' disease, familial exudative vitreoretinopathy and retinopathy of incontinentia pigmenti. The limited trials of anti-VEGF therapy for pediatric vitreoretinal diseases are promising, although more extensive controlled trials will be needed to confirm their safety and efficacy. This paper will examine the current evidence for use of anti-VEGF therapy in a number of pediatric vitreoretinal disorders and describe a case of anti-VEGF therapy in retinopathy of incontinentia pigmenti.
The goal of this study was to review, evaluate, and perform a meta-analysis on the current litera... more The goal of this study was to review, evaluate, and perform a meta-analysis on the current literature that reports rates of postoperative endophthalmitis after small gauge transconjunctival sutureless vitrectomy (TSV) and compare it to 20-gauge pars plana vitrectomy (20G PPV). We performed an extensive review of the current literature. We included only large comparative institutional reviews. Meta-analysis of these reviews was performed. We found six large retrospective comparative cases series on the 25-gauge (25G) TSV as compared to 20G PPV. The test for homogeneity for the meta-analysis indicates that the studies are not homogeneous and therefore the evidence is tentative. We cannot conclude that 25G TSV has a higher rate of postoperative endophthalmitis compared to the 20G PPV. Future retrospective or prospective trials need to take into consideration multiple factors.
We treated two brothers who had a hemorrhagic macular lesion in one eye; a similar problem affect... more We treated two brothers who had a hemorrhagic macular lesion in one eye; a similar problem affected the fellow eye of both patients within eight months. Generalized fine granularity of the retinal pigment epithelium and peripheral iris transillumination defects were observed in both siblings. A study of the family suggested that the disorder was dominantly inherited and probably was Sorsby's pseudoinflammatory macular dystrophy. The macular lesions in one brother were treated by argon green laser photocoagulation and in the other brother by krypton red laser photocoagulation. Although the brother treated by krypton red laser photocoagulation attained better final visual function, additional differences in treatment methods also may have contributed to the final outcome.
The clinical phenotype of advanced stage retinopathy of prematurity (ROP, stages 4 and 5) cannot ... more The clinical phenotype of advanced stage retinopathy of prematurity (ROP, stages 4 and 5) cannot be replicated in an animal model. To dissect the molecular events that can lead up to advanced ROP, we examined subretinal fluid (SRF) and surgically dissected retrolental membranes from patients with advanced ROP to evaluate its influences on cell proliferation, angiogenic properties, and macrophage polarity. We compared our findings to SRF collected from patients with uncomplicated rhegmatogenous retinal detachment (RD) without proliferative vitreoretinopathy and surgically dissected epiretinal membrane from eyes with macular pucker. All subretinal fluid samples were equalized for protein. The angiogenic potential of SRF from ROP eyes was measured using a combination of capillary cord formation in a fibrin clot assay, and its proliferative effect was tested with a DNA synthesis of human retinal microvascular endothelial cells. Findings were compared with SRF collected from participants...
... himoto T, Nakanishi K, Matsui K, Hirose S, Hiroishi K, Tanaka T, Hada T, Hamaoka T, Higashino... more ... himoto T, Nakanishi K, Matsui K, Hirose S, Hiroishi K, Tanaka T, Hada T, Hamaoka T, Higashino K (1990) IL5 upregulates but IL4 downregulates IL2R expression on a ... awski JB, Jelinek DF, Lipsky PE (1989) Immunomodulatory role of IL4 on the secretion of Ig by human B cells. ...
In this report, we describe a 43- to 50-kDa protein, which may function as a costimulatory molecu... more In this report, we describe a 43- to 50-kDa protein, which may function as a costimulatory molecule for full activation of human T cells. This Ag, defined by a mouse monoclonal antibody (mAb) anti-4F9, is primarily distributed on "helper/inducer" or "memory" CD4+CD45RO+ subset. Like mAbs against many other accessory/costimulatory molecules, coimmobilization of anti-4F9 with anti-CD3 resulted in synergistic T cell proliferation. In addition, immobilized anti-4F9 on plastic plates induced T cell spreading characterized by the development of prominent dendritic processes. A cDNA encoding the 4F9 Ag was isolated from a cDNA library constructed from PHA/PMA-activated T cells using a COS cell expression system. The sequence of the cDNA and a homology search revealed that the 4F9 Ag was identical to R2, a molecule recently cloned by subtractive hybridization. The 4F9/R2 Ag belongs to a newly identified supergene family (tetra spans transmembrane protein family) characterized by four putative transmembrane domains which are highly conserved between the members of this family. Based upon the phenotypical and functional studies described here, we propose that the 4F9 Ag is an integral membrane protein which can transmit signals involved in T cell proliferation and adhesion. The preferential distribution of this molecule on the CD4+CD45RO+ subset of T cells may contribute to the distinct activation profile and functional repertoire of these cells.
in life when bone catabolism supersedes anabolism, the significant loss of bone mass (osteoporosi... more in life when bone catabolism supersedes anabolism, the significant loss of bone mass (osteoporosis) becomes a common medical problem ). tures (NIH Consensus Development Panel on Osteopo-Shauna Heeger, George Sabatakos, Suneel Apte, rosis Prevention, 2001). The worldwide annual incidence William N. Adkins, Jeremy Allgrove, of osteoporotic hip fracture exceeds 1.7 million cases Mine Arslan-Kirchner, Jennifer A. Batch, (http://www.who.int/inf-pr-1 999/en/pr99-58.html). Peter Beighton, Graeme C. M. Black, Numerous factors have been implicated in the develop-Richard G. Boles, Laurence M. Boon, ment of osteoporosis. Family and epidemiological stud-Carla Borrone, Han G. Brunner, Georges F. Carle, ies indicate that the peak bone mass attained during Bruno Dallapiccola, Anne De Paepe, Barbara Floege, growth is an important risk factor (NIH Consensus Devel-Melissa Lees Halfhide, Bryan Hall, opment Panel on Osteoporosis Prevention, 2001; See-Raoul C. Hennekam, Tatsuo Hirose, Ab Jans, man et al., 1994). Harald Jü ppner, Chong Ae Kim, Kim Keppler-Noreuil, Two principal cell types are responsible for the depo-Alfried Kohlschuetter, Didier LaCombe, sition and degradation of bone matrix. The bone-forming Marie Lambert, Emmanuelle Lemyre, cells, osteoblasts, differentiate from mesenchymal stem Tom Letteboer, Leena Peltonen, cells at sites of membranous bone formation, in perios-Rajkumar S. Ramesar, Marta Romanengo, teum of endochondral bones, and in bone marrow Hannu Somer, Elisabeth Steichen-Gersdorf, stroma (Aubin, 1998). The bone-degrading cells, osteo-Beat Steinmann, Beth Sullivan, clasts, differentiate from hematopoietic mononuclear Andrea Superti-Furga, Walter Swoboda, cells present in peripheral blood and bone marrow Marie-José van den Boogaard, Wim Van Hul, (Roodman, 1996). In order for bone mass to accrue dur-Miikka Vikkula, Marcela Votruba, Bernhard Zabel, ing the growth and maturation of the skeleton, bone Teresa Garcia, Roland Baron, Bjorn R. Olsen, formation by osteoblasts must exceed bone degradaand Matthew L. Warman 1 tion by osteoclasts. The Osteoporosis-Pseudoglioma Syndrome Children with the autosomal recessive disorder osteo-Collaborative Group 3 porosis-pseudoglioma syndrome (OPPG) (Gong et al., 1996) have very low bone mass and are prone to developing fractures and deformation ( .
Biochemical and Biophysical Research Communications, 1988
The effect of culture supernatants of endothelial cell(EC) lines on the immunoglobulin-M(IgM) syn... more The effect of culture supernatants of endothelial cell(EC) lines on the immunoglobulin-M(IgM) synthesis by human B cell line, SKW6-CL4 cells, was investigated. Supernatants of human EC stimulated IgM synthesis, as high as 6-fold, but supernatants of bovine EC did not. This enhancing activity was completely blocked by addition of anti-human B cell stimulatory factor-2/interleukin-6 (BSF-2/IL-6) antibody. These data suggest that human EC might participate in the human antibody production system by producing soluble factor, BSF-2/IL-6.
We performed selective and continuous immunoadsorption of anti-DNA antibodies from the blood of 6... more We performed selective and continuous immunoadsorption of anti-DNA antibodies from the blood of 6 patients with systemic lupus erythematosus, using a newly developed extracorporeal immunoadsorption system equipped with twin dextran sulfate-cellulose columns with an automated regenerating unit. Levels of anti-DNA, which were initially high, were rapidly diminished after 2-4 apheresis procedures in each patient; in 3 patients with proteinuria and 4 patients with lymphocytopenia, these symptoms also improved. Analysis of the kinetics and the adsorbed amounts of anti-DNA during the apheresis indicates that both the intravascular and the extravascular pool of anti-DNA are reduced with this potent apheresis technique.
A 70-year-old woman with rheumatoid arthritis (RA) and secondary amyloidosis presented repeated c... more A 70-year-old woman with rheumatoid arthritis (RA) and secondary amyloidosis presented repeated consciousness loss. The pathological findings at autopsy revealed multi-organic deposits of amyloid A-protein and so-called 'plexiform change' of blood vessels in bilateral temporal lobes. The arterial plexiform change, which is found in the lung specimen of primary pulmonary hypertension, might be a new pathological cerebrovascular change associated with RA.
Although the roles of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF... more Although the roles of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) in angiogenesis are well described , the putative roles of these factors in retinopathy of prematurity (ROP) remain unknown. We evaluated VEGF and HGF protein levels in subretinal fluid of eyes with ROP , and expression of their corresponding receptors in retrolental membranes associated with stage 5 ROP. We examined subretinal fluid samples from eyes using rhegmatogenous retinal detachment as a control. VEGF and HGF were differentially elevated in eyes with ROP. In Stage 5 ROP (n ؍ 22) , the mean VEGF and HGF levels were 14.77 ؎ 14.01 ng/ml and 16.56 ؎ 9.62 ng/ml , respectively. Interestingly , in patients with active stage 4 ROP , mean VEGF levels were highly elevated (44.16 ؎ 18.72 ng/ml) , whereas mean HGF levels remained very low (4.77 ؎ 2.50 ng/ml). Next, we investigated in vivo expression of VEGF receptor-2 and HGF receptor in retrolental membranes from 16 patients with stage 5 ROP. Both VEGF receptor-2 and HGF receptor proteins were detected mainly in posterior portions of the membrane as well as in vessel walls and along the retinal interface where angiogenesis was active. These findings together suggest that VEGF and HGF play important roles in the pathogenesis of ROP.
Stargardt disease is the most common form of autosomal recessive macular dystrophy. Mutation in t... more Stargardt disease is the most common form of autosomal recessive macular dystrophy. Mutation in the ABCA4 gene (ABCR protein) is responsible for disease manifestation in more than 95% of Stargardt patients. ABCA4 codes for a member of the ATP binding cassette transmembrane protein involved in the transport of all-trans retinal. Dysfunction in this protein causes accumulation of lipofuscin, which is toxic to the RPE and photoreceptors. Presenting symptoms, fundus appearance, and progression of the disease are widely variable in this disease. Different imaging modalities have been utilized to study the presentation and evolution of fundus changes. Although there is ongoing research to better understand the disease process and ways to alternate its path, currently there is no treatment for Startgardt patients.
The advent of optical coherence tomography (OCT) technology has greatly enhanced our understandin... more The advent of optical coherence tomography (OCT) technology has greatly enhanced our understanding of vitreoretinal diseases; it has become a routine diagnostic imaging method for the evaluation of vitreoretinal abnormalities and injuries in adult and pediatric patients. The use of OCT has recently been extended beyond the offices and clinics to perioperative and intraoperative settings. The new development in high-resolution and high-speed spectral domain OCT, along with the improvement in portability, has made the device more valuable than ever before. Our article summarizes the current development of the portable OCT devices in clinical, perioperative, and intraoperative settings in the management of vitreoretinal diseases.
In order to clarify the role of CD5 antigen on B cell in autoimmunity, we examined B cells from p... more In order to clarify the role of CD5 antigen on B cell in autoimmunity, we examined B cells from patients with rheumatoid arthritis (RA). The percentages of CD5 positive B cells were increased in peripheral blood from RA compared with normal. Normal and RA B cells were stimulated with two kinds of monoclonal antibodies to CD5 (Leu-1, SL-1) which recognize different epitopes. RA B cells proliferated and secreted IgM by CD5 antibody stimulation in combination with IL-1. Our observations imply that CD5 positive B cells in RA are in their differentiation stage and that CD5 antigen might be one of the triggers to activate CD5 positive B cells in vivo to produce autoantibody.
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1990
The uptake of LDL and acetylated LDL and the ability of cholesteryl ester accumulation by cells o... more The uptake of LDL and acetylated LDL and the ability of cholesteryl ester accumulation by cells of a human monocytic cell line, U937, has been characterized by flow cytometric assay using a fluorescent probe, DiI, and by high-performance liquid chromatography (HPLC). The increase of mean fluorescence intensity of U937 incubated with DiI-labeled lipoproteins demonstrates that this cell line could incorporate DiI-AcLDL, as well as DiI-labeled LDL. Competition and saturation studies indicate that the manner of taking up DiI-AcLDL is receptor-mediated. While differentiated U937 incubated with 16 nM phorbol myristate acetate for 24 h took up little DiI-AcLDL, HPLC analysis confirmed that intracellular free and esterified cholesterols significantly increase in the U937 cells incubated with AcLDL or LDL. The ability of mouse peritoneal macrophage to abundantly accumulate at least five kinds of cholesteryl ester were also shown in this analysis. In contrast, in U937 cells, free fatty acids are incorporated into various substances rather than into cholesteryl esters (as revealed by HPLC analysis), so that the cholesterol in AcLDL taken up by U937 cells is not synthesized into cholesteryl esters to any great extent.
While angiogenesis inhibitors are already widely used to treat retinal disease in adults, only li... more While angiogenesis inhibitors are already widely used to treat retinal disease in adults, only limited reports are currently available for the use of anti-VEGF in pediatric vitreoretinal diseases such as retinopathy of prematurity, Coats' disease, familial exudative vitreoretinopathy and retinopathy of incontinentia pigmenti. The limited trials of anti-VEGF therapy for pediatric vitreoretinal diseases are promising, although more extensive controlled trials will be needed to confirm their safety and efficacy. This paper will examine the current evidence for use of anti-VEGF therapy in a number of pediatric vitreoretinal disorders and describe a case of anti-VEGF therapy in retinopathy of incontinentia pigmenti.
The goal of this study was to review, evaluate, and perform a meta-analysis on the current litera... more The goal of this study was to review, evaluate, and perform a meta-analysis on the current literature that reports rates of postoperative endophthalmitis after small gauge transconjunctival sutureless vitrectomy (TSV) and compare it to 20-gauge pars plana vitrectomy (20G PPV). We performed an extensive review of the current literature. We included only large comparative institutional reviews. Meta-analysis of these reviews was performed. We found six large retrospective comparative cases series on the 25-gauge (25G) TSV as compared to 20G PPV. The test for homogeneity for the meta-analysis indicates that the studies are not homogeneous and therefore the evidence is tentative. We cannot conclude that 25G TSV has a higher rate of postoperative endophthalmitis compared to the 20G PPV. Future retrospective or prospective trials need to take into consideration multiple factors.
We treated two brothers who had a hemorrhagic macular lesion in one eye; a similar problem affect... more We treated two brothers who had a hemorrhagic macular lesion in one eye; a similar problem affected the fellow eye of both patients within eight months. Generalized fine granularity of the retinal pigment epithelium and peripheral iris transillumination defects were observed in both siblings. A study of the family suggested that the disorder was dominantly inherited and probably was Sorsby's pseudoinflammatory macular dystrophy. The macular lesions in one brother were treated by argon green laser photocoagulation and in the other brother by krypton red laser photocoagulation. Although the brother treated by krypton red laser photocoagulation attained better final visual function, additional differences in treatment methods also may have contributed to the final outcome.
The clinical phenotype of advanced stage retinopathy of prematurity (ROP, stages 4 and 5) cannot ... more The clinical phenotype of advanced stage retinopathy of prematurity (ROP, stages 4 and 5) cannot be replicated in an animal model. To dissect the molecular events that can lead up to advanced ROP, we examined subretinal fluid (SRF) and surgically dissected retrolental membranes from patients with advanced ROP to evaluate its influences on cell proliferation, angiogenic properties, and macrophage polarity. We compared our findings to SRF collected from patients with uncomplicated rhegmatogenous retinal detachment (RD) without proliferative vitreoretinopathy and surgically dissected epiretinal membrane from eyes with macular pucker. All subretinal fluid samples were equalized for protein. The angiogenic potential of SRF from ROP eyes was measured using a combination of capillary cord formation in a fibrin clot assay, and its proliferative effect was tested with a DNA synthesis of human retinal microvascular endothelial cells. Findings were compared with SRF collected from participants...
... himoto T, Nakanishi K, Matsui K, Hirose S, Hiroishi K, Tanaka T, Hada T, Hamaoka T, Higashino... more ... himoto T, Nakanishi K, Matsui K, Hirose S, Hiroishi K, Tanaka T, Hada T, Hamaoka T, Higashino K (1990) IL5 upregulates but IL4 downregulates IL2R expression on a ... awski JB, Jelinek DF, Lipsky PE (1989) Immunomodulatory role of IL4 on the secretion of Ig by human B cells. ...
In this report, we describe a 43- to 50-kDa protein, which may function as a costimulatory molecu... more In this report, we describe a 43- to 50-kDa protein, which may function as a costimulatory molecule for full activation of human T cells. This Ag, defined by a mouse monoclonal antibody (mAb) anti-4F9, is primarily distributed on "helper/inducer" or "memory" CD4+CD45RO+ subset. Like mAbs against many other accessory/costimulatory molecules, coimmobilization of anti-4F9 with anti-CD3 resulted in synergistic T cell proliferation. In addition, immobilized anti-4F9 on plastic plates induced T cell spreading characterized by the development of prominent dendritic processes. A cDNA encoding the 4F9 Ag was isolated from a cDNA library constructed from PHA/PMA-activated T cells using a COS cell expression system. The sequence of the cDNA and a homology search revealed that the 4F9 Ag was identical to R2, a molecule recently cloned by subtractive hybridization. The 4F9/R2 Ag belongs to a newly identified supergene family (tetra spans transmembrane protein family) characterized by four putative transmembrane domains which are highly conserved between the members of this family. Based upon the phenotypical and functional studies described here, we propose that the 4F9 Ag is an integral membrane protein which can transmit signals involved in T cell proliferation and adhesion. The preferential distribution of this molecule on the CD4+CD45RO+ subset of T cells may contribute to the distinct activation profile and functional repertoire of these cells.
in life when bone catabolism supersedes anabolism, the significant loss of bone mass (osteoporosi... more in life when bone catabolism supersedes anabolism, the significant loss of bone mass (osteoporosis) becomes a common medical problem ). tures (NIH Consensus Development Panel on Osteopo-Shauna Heeger, George Sabatakos, Suneel Apte, rosis Prevention, 2001). The worldwide annual incidence William N. Adkins, Jeremy Allgrove, of osteoporotic hip fracture exceeds 1.7 million cases Mine Arslan-Kirchner, Jennifer A. Batch, (http://www.who.int/inf-pr-1 999/en/pr99-58.html). Peter Beighton, Graeme C. M. Black, Numerous factors have been implicated in the develop-Richard G. Boles, Laurence M. Boon, ment of osteoporosis. Family and epidemiological stud-Carla Borrone, Han G. Brunner, Georges F. Carle, ies indicate that the peak bone mass attained during Bruno Dallapiccola, Anne De Paepe, Barbara Floege, growth is an important risk factor (NIH Consensus Devel-Melissa Lees Halfhide, Bryan Hall, opment Panel on Osteoporosis Prevention, 2001; See-Raoul C. Hennekam, Tatsuo Hirose, Ab Jans, man et al., 1994). Harald Jü ppner, Chong Ae Kim, Kim Keppler-Noreuil, Two principal cell types are responsible for the depo-Alfried Kohlschuetter, Didier LaCombe, sition and degradation of bone matrix. The bone-forming Marie Lambert, Emmanuelle Lemyre, cells, osteoblasts, differentiate from mesenchymal stem Tom Letteboer, Leena Peltonen, cells at sites of membranous bone formation, in perios-Rajkumar S. Ramesar, Marta Romanengo, teum of endochondral bones, and in bone marrow Hannu Somer, Elisabeth Steichen-Gersdorf, stroma (Aubin, 1998). The bone-degrading cells, osteo-Beat Steinmann, Beth Sullivan, clasts, differentiate from hematopoietic mononuclear Andrea Superti-Furga, Walter Swoboda, cells present in peripheral blood and bone marrow Marie-José van den Boogaard, Wim Van Hul, (Roodman, 1996). In order for bone mass to accrue dur-Miikka Vikkula, Marcela Votruba, Bernhard Zabel, ing the growth and maturation of the skeleton, bone Teresa Garcia, Roland Baron, Bjorn R. Olsen, formation by osteoblasts must exceed bone degradaand Matthew L. Warman 1 tion by osteoclasts. The Osteoporosis-Pseudoglioma Syndrome Children with the autosomal recessive disorder osteo-Collaborative Group 3 porosis-pseudoglioma syndrome (OPPG) (Gong et al., 1996) have very low bone mass and are prone to developing fractures and deformation ( .
Biochemical and Biophysical Research Communications, 1988
The effect of culture supernatants of endothelial cell(EC) lines on the immunoglobulin-M(IgM) syn... more The effect of culture supernatants of endothelial cell(EC) lines on the immunoglobulin-M(IgM) synthesis by human B cell line, SKW6-CL4 cells, was investigated. Supernatants of human EC stimulated IgM synthesis, as high as 6-fold, but supernatants of bovine EC did not. This enhancing activity was completely blocked by addition of anti-human B cell stimulatory factor-2/interleukin-6 (BSF-2/IL-6) antibody. These data suggest that human EC might participate in the human antibody production system by producing soluble factor, BSF-2/IL-6.
We performed selective and continuous immunoadsorption of anti-DNA antibodies from the blood of 6... more We performed selective and continuous immunoadsorption of anti-DNA antibodies from the blood of 6 patients with systemic lupus erythematosus, using a newly developed extracorporeal immunoadsorption system equipped with twin dextran sulfate-cellulose columns with an automated regenerating unit. Levels of anti-DNA, which were initially high, were rapidly diminished after 2-4 apheresis procedures in each patient; in 3 patients with proteinuria and 4 patients with lymphocytopenia, these symptoms also improved. Analysis of the kinetics and the adsorbed amounts of anti-DNA during the apheresis indicates that both the intravascular and the extravascular pool of anti-DNA are reduced with this potent apheresis technique.
Uploads
Papers by Tatsuo Hirose