Interferon-gamma (IFN-γ) plays a crucial role in resistance to mycobacterial infections, as it is... more Interferon-gamma (IFN-γ) plays a crucial role in resistance to mycobacterial infections, as it is a regulatory cytokine that acts as a pro-inflammatory mediator. Consequently, variants in the gene encoding this cytokine may be associated with a high risk of contracting pulmonary tuberculosis. The present study aimed to investigate the genetic susceptibility of polymorphisms in the gene coding for IFN-γ to infection by Mycobacterium tuberculosis in Burkina Faso. This cross-sectional study was conducted from May 2023 to January 2024. Venous blood was collected from suspected cases. Tuberculosis was confirmed by GeneXpert (CEPHEID). Human genomic DNA was extracted using the salting-out extraction technique, followed by the amplification and genotyping of IFN-γ gene polymorphisms,through the conventional PCR. Statistical analyses were performed using the SPSS and Epi info software. A total of 168 participants were included in the study, with an average age of 38.58 ±14.88, the majority of whom were men (76.19%). In our study population, 73.2% (123/168) were confirmed positive for tuberculosis. Some 46.4% (78/168) of the previous cases were contacts. Of these contact cases, 82.05% (64/78) were GeneXpert positive. The genotypic frequencies of the IFN-γ gene were distributed as follows: 73.3% (AA), 21.8% (AT) and 4.9% (TT), with a frequency of 84.2% for the A allele versus 15.8% for the mutated T allele. No statistically significant association was found between IFN-γ gene polymorphisms and M. tuberculosis infection in Burkina Faso. IFN-γ gene polymorphisms (IFN +874T/A) do not appear to be associated with M. tuberculosis infection in Burkina Faso.
Introduction: Pulmonary tuberculosis and COVID-19 have shared some similarities in term of clinic... more Introduction: Pulmonary tuberculosis and COVID-19 have shared some similarities in term of clinical manifestations and modes of transmission. COVID-19 could increase the mortality rate of tuberculosis. Consequently, pulmonary tuberculosis and COVID-19 co-infection could be a major public health concern. The aim of this study was to establish the prevalence of pulmonary tuberculosis and COVID-19 coinfection in the city of Ouagadougou, Burkina Faso. Method: Mycobacterium tuberculosis DNA, was detected using GeneXpert (Cepheid, USA), on the sputum of suspected pulmonary tuberculosis. Anti-SARS-CoV-2 antibodies (IgM and IgG) was detected using the Accu-Tell rapid kit (AccuBio Techn Co, Ltd kit) on the blood plasma of suspected pulmonary tuberculosis cases. SARS-CoV-2 RNA amplification
Introduction: The tuberculosis (TB) and HIV co-infection is a deadly combination, each accelerati... more Introduction: The tuberculosis (TB) and HIV co-infection is a deadly combination, each accelerating the progression of the other. TB, caused by Mycobacterium tuberculosis (M. tuberculosis), is the leading cause of deaths among
Cervical cancer is the fourth most common cancer worldwide, accounting for 6.8% of new cancer cas... more Cervical cancer is the fourth most common cancer worldwide, accounting for 6.8% of new cancer cases and 8.1% of cancer-related deaths. About 85% of these deaths occurred in low-and middle-income countries. The aim of this study was to assess the frequency and distribution of the human papillomavirus (HPV) genotypes in women showing cytological abnormalities of the cervix at the Sourô SANOU University Hospital (CHUSS) in Bobo-Dioulasso, Burkina Faso. This is a descriptive study of women recruited at the CHUSS. The cervico-uterine smear examination was carried out at the CHUSS Anatomy and Pathology Department for cervical cancer screening. The data were collected from women with atypical cells on their cervico-uterine smear. Cervicovaginal samples were taken from consenting women and HPV genotyping was performed using the HPV Direct FLOW CHIP kit at CERBA. We obtained approval from the ethics committee. The data were analyzed using the SPSS 26 software. The results of the study showed that 67.79% of the participants were aged between 50 and 65, a group that is particularly vulnerable to persistent infection with high-risk oncogenic HPV genotypes. Of the women
Epidemiological profile of human papillomavirus infections and cervical cancer prevention among s... more Epidemiological profile of human papillomavirus infections and cervical cancer prevention among sexually active women in Burkina Faso: Literature Review. Medical Research Archives, [online] 12(11).
Introduction: Burkina Faso, a West African country, has also seen a resurgence in new cases of tu... more Introduction: Burkina Faso, a West African country, has also seen a resurgence in new cases of tuberculosis. It would be useful to have an overview of how prevalence and diagnostic methods have changed in recent years. The aim of this study was to estimate the prevalence of tuberculosis in Burkina Faso over the past 10 years and to summarize the diagnostic methods used. Material and Methods: A review of studies published between 2014 and 2024 on the prevalence of tuberculosis in Burkina Faso and the diagnostic methods used were carried out using the "Preferred Reporting Items for Systematic Reviews and Meta-Analysis" (PRISMA). Relevant publications were selected from the PubMed/Medline, Embase, Google Scholar, and ScienceDirect databases. Results: Most of the studies on the prevalence of pulmonary tuberculosis in Burkina Faso found in this review were generally hospital-based and included both child and adult populations. Prevalence rates ranged from 15% to 63.36%, with a calculated overall prevalence of 31.58% [(n = 348; 95% CI (26.86-36.30)] in the studies examined. The majority of authors used microscopy, which is the standard diagnostic method. Molecular techniques such as GeneXpert were associated with microscopy in over 70% of studies, compared with less than 30% for culture. Conclusion: At the end of this study, the number of reported cases fluctuated over the years. In terms of diagnostic methods, in the last 10 years, more specific molecular techniques have been introduced, along with the use of alternative samples to sputum for the detection of Mycobacterium tuberculosis.
International Journal of Current Microbiology and Applied Sciences
Helicobacter pylori infection leads to gastritis that can evolve into severe forms of ulceration ... more Helicobacter pylori infection leads to gastritis that can evolve into severe forms of ulceration and malignant transformation. Its diagnosis is most often invasive. Our study aim was to characterize by a non-invasive method, Helicobacter pylori virulence genes iceA, oipA, and cagE in stool samples from Burkina Faso. Methods: This cross-sectional study took place from September 2020 to February 2021 and involved 250 patients. Each patient provided a stool sample, which was used to perform an Helicobacter pylori antigenic detection test and molecular analysis by polymerase chain reaction (PCR) to detect its virulence genes cagE, iceA, and oipA. Results: The results showed a Helicobacter pylori infection rate of 89.6%. The virulence genes cagE, iceA1, iceA2, and oipA frequencies were 75.4%, 3.12%, 3.12%, and1.78% respectively. Our bacterial population had only one iceA genotype. The cagE gene was significantly linked with H. pylori infection (p-value=0.001). We also found a significant...
Introduction: Burkina Faso experiences regular cases of Streptococcus pneumoniae meningitis. As p... more Introduction: Burkina Faso experiences regular cases of Streptococcus pneumoniae meningitis. As part of the strategy to reduce cases of meningitis, the 13-valent pneumococcal conjugate vaccine (PCV-13) has been introduced in the Expanded Programme on Immunisation (EPI). Despite these efforts, there are some cases of pneumococcal meningitis including both vaccine and non-vaccine serotypes. The objective of this study was to describe the pneumococcal sequence types (ST) circulating in Burkina Faso before the introduction of the 13-valent pneumococcal conjugate vaccine (PCV-13). Methods: It was a descriptive cross-sectional study that took place from 27 th October 2013 to 7 th January 2014. S. pneumoniae strains were collected in Burkina Faso and Multi Locus Sequence Typing (MLST) was performed at the Pneumococcal Laboratory at the Centers for Disease Control and Prevention (CDC) in the USA (United States of America). MLST consists of 4 steps: amplification, purification, sequencing and interpretative reading of the results. The amplification used 7 primers consisting of sequences of aroe, gdh, gki, recP, spi, xpt, ddl genes. Results: Of 37 strains tested, 10 serotypes were identified. Serotype 1 was prevalent in 48.7% (18/37) followed by serotype 25F in 10.8% (4/37). Serotypes 5 and 12F/12A/12B/44/46 were 8.1% (3/37) each. Serotype 1 contained 5 STs including ST303 24.3% (9/37), ST217 8.1% (3/37) and ST618 8.1% (3/37); followed by serotype 25F with ST105 10.8%
Introduction: Meningitis caused by Neisseria meningitidis constitutes a burden for the countries ... more Introduction: Meningitis caused by Neisseria meningitidis constitutes a burden for the countries in the meningitis belt of sub-Saharan in general and particularly for Burkina Faso. In 2019 the Diapaga health district experienced a meningitis epidemic due to N. meningitidis serogroup C. Methods: This is a cross-sectional study with a descriptive aim in the health district of Diapaga where all cases of meningitis were included in this work. Rapid diagnostic tests (RDTs), culture as well as real-time PCR were used for the biological analysis of cerebral spinal fluid (CSF) samples. Results: Of 155 CSF samples analysed, 42% (65/155) were tested positve. Of them, N. meningitidis C accounted for 83% of all positive cases. Likewise, all thirteen (13) NmC strains were susceptible to oxacillin, ceftriaxone, penicillin and chloramphenicol. All strains of NmC belonged to the sequence type (ST) 10 217 and to the clonal complex (CC) 10 217. These CCs belonged to the same variant PorA type: P1.21-15.16; FetA type: F1-7; PorB type: 3-463. Conclusion: Burkina Faso had known an epidemic of meningitis caused by NmC in 2019 in the health district of Diapaga. This outbreak was contained in time due to the performance of the epidemiological surveillance system which made it possible to investigate on time and introduce the vaccine against the pathogen NmC.
ABSTRACTImportanceAccurate diagnosis of tuberculosis (TB) infection can be achieved with interfer... more ABSTRACTImportanceAccurate diagnosis of tuberculosis (TB) infection can be achieved with interferon-γ release assays. Their performance can be improved by utilizing fully automated, single-patient formats.ObjectiveEstablish clinical thresholds for a new interferon-γ release assay, the VIDAS® TB-IGRA, and compare diagnostic performance in detecting tuberculosis infection and disease with the established QuantiFERON-TB Gold Plus (QFT-Plus).DesignPreliminary diagnostic performance study (October 2nd, 2019–February 20th, 2020).SettingMulticenter.ParticipantsParticipants were divided into TB disease, high-risk, and low-risk populations. The confirmed TB disease population included 107 patients. The high-risk population included 162 individuals with flagged risk factors on a questionnaire but without objective clinical confirmation of TB. The Low-risk population included 117 healthy blood donors from the French National Blood Bank.ExposuresTuberculosis.Main Outcomes and MeasuresPositive a...
The diverse clinical presentation of Helicobacter pylori (H. pylori) infection results from the i... more The diverse clinical presentation of Helicobacter pylori (H. pylori) infection results from the interaction between bacterial virulence, host genetics, socio-demographic and environmental factors. This study aimed to characterize Helicobacter pylori virulence genes and the associated behavioral factors among dyspeptic patients in Burkina Faso. Two hundred and fifty (250) stool samples were collected from patients with dyspepsia seen at health centers in Ouagadougou, Burkina Faso. Bacterial deoxyribonucleic acid (DNA) was extracted using a commercial kit. Virulence genes were detected using conventional multiplex Polymerase Chain Reaction with specific primers. The overall prevalence of Helicobacter pylori of the 250 participants was 91.20%. Ca-gA virulence gene was present among 20.19% of individuals, while babA2 and vacA were detected respectively among 9.65% and 67.54% of the population positive for Helicobacter pylori. Among vacA subtypes, vacAs1 was the most frequent, with 39.04%, followed by vacAi1 (19.74%), vacAi2 (17.54%), and vacAs2 with 10.96%. Regarding vacAm1 and vacAm2, they were less frequent at 6.14% each. "Handwashing three times or less per day" significantly increased the risk of having vacAi2 allele and H. pylori rRNA16s, with p-values of 0.013 and 0.020, respectively. The consumption of non-tap water increases the risk of carrying the cagA virulence gene. Additionally, H. pylori-positive
IP international journal of medical microbiology and tropical diseases, Apr 15, 2023
Abstract Background: This study aimed to evaluate the correlation between suspected COVID-19 symp... more Abstract Background: This study aimed to evaluate the correlation between suspected COVID-19 symptoms and RT-PCR results in the diagnosis of SARS-CoV-2 infection in Burkina Faso. Materials and Methods: We analyzed SARS-CoV-2 RT-PCR routine diagnostic data in Burkina Faso. Data were collected from March 9, 2020 to September 30, 2020 in the framework of the COVID-19 surveillance. Sensitivity, specificity, predictive values, and Kappa concordance were used to check the correlation between COVID-19 symptoms and the RT-PCR results. Results: A total of 2217 participants were tested for COVID-19 using RT-PCR, of them 779 COVID-19 positive. The mean age of the participants was 38.7± 17.69 years. Suspected symptoms presented by participants were fever (40.4%), cough (38.6%), asthenia (27.3%), headache (23.6%), dyspnea (20.8%), and odynophagia (16.3%). The sensitivity of presence of at least a clinical sign compared to RT-PCR results was 62.13% and the specificity was 39.85%. The kappa agreement between the presence of COVID-19 suspected symptoms and RT-PCR results was 0.017. The presence of aguesia and/or anosmia in patients induced a positive predictive value of RT-PCR of 91.30%. Conclusion: The correlation between the COVID-19 symptoms and RT-PCR results in the diagnosis of COVID-19 was very weak. The present study confirms that most clinical signs associated with SARS-CoV-2 infection are not specific to COVID-19, hence the need to always combine RT-PCR or other biological tests with the clinical diagnosis. However, aguesia and anosmia are of interest with a high degree of RT-PCR positivity when present in a COVID-19 suspected patient. Keywords: Diagnosis, Symptoms, Correlation, COVID-19, SARS-CoV-2, RT-PCR
Background The aim of this study was to evaluate the performance of ten (10) SARS-CoV-2 serologic... more Background The aim of this study was to evaluate the performance of ten (10) SARS-CoV-2 serological rapid diagnostic tests in comparison with the WANTAI SARS-CoV-2 Ab ELISA test in a laboratory setting. Materials and methods Ten (10) SARS-CoV-2 serological rapid diagnostic tests (RDTs) for SARS-CoV-2 IgG/IgM were evaluated with two (2) groups of plasma tested positive for one and negative for the other with the WANTAI SARS-CoV-2 Ab ELISA. The diagnostic performance of the SARS-CoV-2 serological RDTs and their agreement with the reference test were calculated with their 95% confidence intervals. Results The sensitivity of serological RDTs ranged from 27.39 to 61.67% and the specificity from 93.33 to 100% compared to WANTAI SARS-CoV-2 Ab ELISA test. Of all the tests, two tests (STANDARD Q COVID-19 IgM/IgG Combo SD BIOSENSOR and COVID-19 IgG/IgM Rapid Test (Zhejiang Orient Gene Biotech Co., Ltd)) had a sensitivity greater than 50%. In addition, all ten tests had specificity greater tha...
Selon l’organisation Mondiale de la Sante (oMS), l’afrique Sub-saharienne est l’une des regions ... more Selon l’organisation Mondiale de la Sante (oMS), l’afrique Sub-saharienne est l’une des regions les plus touchees par les infections sexuellement transmissibles (iST). au Burkina Faso, la prise en charge des infections genitales repose sur une approche syndromique, et les donnees sur leurs etiologies sont rares. Cette etude a pour objectif d’etablir le profil microbiologique des infections genitales basses chez patientes âgees d’au moins 15 ans dans la ville de Bobo-dioulasso. il s’est agi d’une transversale et prospective qui s’est deroulee d’octobre 2014 a Juillet 2015 dans le laboratoire d’analyse de biologie medicale eXalaB. Chez chaque femme consentante se presentant avec un bulletin d’examen, un prelevement cervico-vaginal a ete realise, examine a la microscopie, puis mis en culture pour l’identification des pathogenes. la susceptibilite aux antimicrobiens a ete determinee par la methode de diffusion sur gelose selon les recommandations(2013) du comite de l’antibiogra...
This study aimed to evaluate the diagnostic performance of commercially rapid point-of-care (POC)... more This study aimed to evaluate the diagnostic performance of commercially rapid point-of-care (POC) tests used for HCV antibodies detection. This is a case-control study conducted in Ouagadougou between December 2014 and January 2015. Three POC for HCV antibodies detection (SD Bioline HCV test®, Anti-HCV dipstick® and First response® HCV card test) marketed in Burkina Faso were evaluated. Architect anti-HCV assay and ImmunoComb® II HCV were combined and used as a reference test. All three tests were evaluated with a panel of 62 anti-HCV positive sera and 62 anti-HCV negative sera. The tests performance was calculated using the software OpenEpi. The three rapid POC tests had a specificity of 100% (95% CI: 94.17-100). However, the sensitivities were 33.87% (95% CI: 23.34-46.28) for the SD Bioline HCV test®, 41.94% (95% CI: 30.48-54.33%) for Anti-HCV dipstick® and 45.16% (95%CI: 33.42-57.47%) for First response® HCV card test. The tests evaluated in this study had good specificity but p...
Interferon-gamma (IFN-γ) plays a crucial role in resistance to mycobacterial infections, as it is... more Interferon-gamma (IFN-γ) plays a crucial role in resistance to mycobacterial infections, as it is a regulatory cytokine that acts as a pro-inflammatory mediator. Consequently, variants in the gene encoding this cytokine may be associated with a high risk of contracting pulmonary tuberculosis. The present study aimed to investigate the genetic susceptibility of polymorphisms in the gene coding for IFN-γ to infection by Mycobacterium tuberculosis in Burkina Faso. This cross-sectional study was conducted from May 2023 to January 2024. Venous blood was collected from suspected cases. Tuberculosis was confirmed by GeneXpert (CEPHEID). Human genomic DNA was extracted using the salting-out extraction technique, followed by the amplification and genotyping of IFN-γ gene polymorphisms,through the conventional PCR. Statistical analyses were performed using the SPSS and Epi info software. A total of 168 participants were included in the study, with an average age of 38.58 ±14.88, the majority of whom were men (76.19%). In our study population, 73.2% (123/168) were confirmed positive for tuberculosis. Some 46.4% (78/168) of the previous cases were contacts. Of these contact cases, 82.05% (64/78) were GeneXpert positive. The genotypic frequencies of the IFN-γ gene were distributed as follows: 73.3% (AA), 21.8% (AT) and 4.9% (TT), with a frequency of 84.2% for the A allele versus 15.8% for the mutated T allele. No statistically significant association was found between IFN-γ gene polymorphisms and M. tuberculosis infection in Burkina Faso. IFN-γ gene polymorphisms (IFN +874T/A) do not appear to be associated with M. tuberculosis infection in Burkina Faso.
Introduction: Pulmonary tuberculosis and COVID-19 have shared some similarities in term of clinic... more Introduction: Pulmonary tuberculosis and COVID-19 have shared some similarities in term of clinical manifestations and modes of transmission. COVID-19 could increase the mortality rate of tuberculosis. Consequently, pulmonary tuberculosis and COVID-19 co-infection could be a major public health concern. The aim of this study was to establish the prevalence of pulmonary tuberculosis and COVID-19 coinfection in the city of Ouagadougou, Burkina Faso. Method: Mycobacterium tuberculosis DNA, was detected using GeneXpert (Cepheid, USA), on the sputum of suspected pulmonary tuberculosis. Anti-SARS-CoV-2 antibodies (IgM and IgG) was detected using the Accu-Tell rapid kit (AccuBio Techn Co, Ltd kit) on the blood plasma of suspected pulmonary tuberculosis cases. SARS-CoV-2 RNA amplification
Introduction: The tuberculosis (TB) and HIV co-infection is a deadly combination, each accelerati... more Introduction: The tuberculosis (TB) and HIV co-infection is a deadly combination, each accelerating the progression of the other. TB, caused by Mycobacterium tuberculosis (M. tuberculosis), is the leading cause of deaths among
Cervical cancer is the fourth most common cancer worldwide, accounting for 6.8% of new cancer cas... more Cervical cancer is the fourth most common cancer worldwide, accounting for 6.8% of new cancer cases and 8.1% of cancer-related deaths. About 85% of these deaths occurred in low-and middle-income countries. The aim of this study was to assess the frequency and distribution of the human papillomavirus (HPV) genotypes in women showing cytological abnormalities of the cervix at the Sourô SANOU University Hospital (CHUSS) in Bobo-Dioulasso, Burkina Faso. This is a descriptive study of women recruited at the CHUSS. The cervico-uterine smear examination was carried out at the CHUSS Anatomy and Pathology Department for cervical cancer screening. The data were collected from women with atypical cells on their cervico-uterine smear. Cervicovaginal samples were taken from consenting women and HPV genotyping was performed using the HPV Direct FLOW CHIP kit at CERBA. We obtained approval from the ethics committee. The data were analyzed using the SPSS 26 software. The results of the study showed that 67.79% of the participants were aged between 50 and 65, a group that is particularly vulnerable to persistent infection with high-risk oncogenic HPV genotypes. Of the women
Epidemiological profile of human papillomavirus infections and cervical cancer prevention among s... more Epidemiological profile of human papillomavirus infections and cervical cancer prevention among sexually active women in Burkina Faso: Literature Review. Medical Research Archives, [online] 12(11).
Introduction: Burkina Faso, a West African country, has also seen a resurgence in new cases of tu... more Introduction: Burkina Faso, a West African country, has also seen a resurgence in new cases of tuberculosis. It would be useful to have an overview of how prevalence and diagnostic methods have changed in recent years. The aim of this study was to estimate the prevalence of tuberculosis in Burkina Faso over the past 10 years and to summarize the diagnostic methods used. Material and Methods: A review of studies published between 2014 and 2024 on the prevalence of tuberculosis in Burkina Faso and the diagnostic methods used were carried out using the "Preferred Reporting Items for Systematic Reviews and Meta-Analysis" (PRISMA). Relevant publications were selected from the PubMed/Medline, Embase, Google Scholar, and ScienceDirect databases. Results: Most of the studies on the prevalence of pulmonary tuberculosis in Burkina Faso found in this review were generally hospital-based and included both child and adult populations. Prevalence rates ranged from 15% to 63.36%, with a calculated overall prevalence of 31.58% [(n = 348; 95% CI (26.86-36.30)] in the studies examined. The majority of authors used microscopy, which is the standard diagnostic method. Molecular techniques such as GeneXpert were associated with microscopy in over 70% of studies, compared with less than 30% for culture. Conclusion: At the end of this study, the number of reported cases fluctuated over the years. In terms of diagnostic methods, in the last 10 years, more specific molecular techniques have been introduced, along with the use of alternative samples to sputum for the detection of Mycobacterium tuberculosis.
International Journal of Current Microbiology and Applied Sciences
Helicobacter pylori infection leads to gastritis that can evolve into severe forms of ulceration ... more Helicobacter pylori infection leads to gastritis that can evolve into severe forms of ulceration and malignant transformation. Its diagnosis is most often invasive. Our study aim was to characterize by a non-invasive method, Helicobacter pylori virulence genes iceA, oipA, and cagE in stool samples from Burkina Faso. Methods: This cross-sectional study took place from September 2020 to February 2021 and involved 250 patients. Each patient provided a stool sample, which was used to perform an Helicobacter pylori antigenic detection test and molecular analysis by polymerase chain reaction (PCR) to detect its virulence genes cagE, iceA, and oipA. Results: The results showed a Helicobacter pylori infection rate of 89.6%. The virulence genes cagE, iceA1, iceA2, and oipA frequencies were 75.4%, 3.12%, 3.12%, and1.78% respectively. Our bacterial population had only one iceA genotype. The cagE gene was significantly linked with H. pylori infection (p-value=0.001). We also found a significant...
Introduction: Burkina Faso experiences regular cases of Streptococcus pneumoniae meningitis. As p... more Introduction: Burkina Faso experiences regular cases of Streptococcus pneumoniae meningitis. As part of the strategy to reduce cases of meningitis, the 13-valent pneumococcal conjugate vaccine (PCV-13) has been introduced in the Expanded Programme on Immunisation (EPI). Despite these efforts, there are some cases of pneumococcal meningitis including both vaccine and non-vaccine serotypes. The objective of this study was to describe the pneumococcal sequence types (ST) circulating in Burkina Faso before the introduction of the 13-valent pneumococcal conjugate vaccine (PCV-13). Methods: It was a descriptive cross-sectional study that took place from 27 th October 2013 to 7 th January 2014. S. pneumoniae strains were collected in Burkina Faso and Multi Locus Sequence Typing (MLST) was performed at the Pneumococcal Laboratory at the Centers for Disease Control and Prevention (CDC) in the USA (United States of America). MLST consists of 4 steps: amplification, purification, sequencing and interpretative reading of the results. The amplification used 7 primers consisting of sequences of aroe, gdh, gki, recP, spi, xpt, ddl genes. Results: Of 37 strains tested, 10 serotypes were identified. Serotype 1 was prevalent in 48.7% (18/37) followed by serotype 25F in 10.8% (4/37). Serotypes 5 and 12F/12A/12B/44/46 were 8.1% (3/37) each. Serotype 1 contained 5 STs including ST303 24.3% (9/37), ST217 8.1% (3/37) and ST618 8.1% (3/37); followed by serotype 25F with ST105 10.8%
Introduction: Meningitis caused by Neisseria meningitidis constitutes a burden for the countries ... more Introduction: Meningitis caused by Neisseria meningitidis constitutes a burden for the countries in the meningitis belt of sub-Saharan in general and particularly for Burkina Faso. In 2019 the Diapaga health district experienced a meningitis epidemic due to N. meningitidis serogroup C. Methods: This is a cross-sectional study with a descriptive aim in the health district of Diapaga where all cases of meningitis were included in this work. Rapid diagnostic tests (RDTs), culture as well as real-time PCR were used for the biological analysis of cerebral spinal fluid (CSF) samples. Results: Of 155 CSF samples analysed, 42% (65/155) were tested positve. Of them, N. meningitidis C accounted for 83% of all positive cases. Likewise, all thirteen (13) NmC strains were susceptible to oxacillin, ceftriaxone, penicillin and chloramphenicol. All strains of NmC belonged to the sequence type (ST) 10 217 and to the clonal complex (CC) 10 217. These CCs belonged to the same variant PorA type: P1.21-15.16; FetA type: F1-7; PorB type: 3-463. Conclusion: Burkina Faso had known an epidemic of meningitis caused by NmC in 2019 in the health district of Diapaga. This outbreak was contained in time due to the performance of the epidemiological surveillance system which made it possible to investigate on time and introduce the vaccine against the pathogen NmC.
ABSTRACTImportanceAccurate diagnosis of tuberculosis (TB) infection can be achieved with interfer... more ABSTRACTImportanceAccurate diagnosis of tuberculosis (TB) infection can be achieved with interferon-γ release assays. Their performance can be improved by utilizing fully automated, single-patient formats.ObjectiveEstablish clinical thresholds for a new interferon-γ release assay, the VIDAS® TB-IGRA, and compare diagnostic performance in detecting tuberculosis infection and disease with the established QuantiFERON-TB Gold Plus (QFT-Plus).DesignPreliminary diagnostic performance study (October 2nd, 2019–February 20th, 2020).SettingMulticenter.ParticipantsParticipants were divided into TB disease, high-risk, and low-risk populations. The confirmed TB disease population included 107 patients. The high-risk population included 162 individuals with flagged risk factors on a questionnaire but without objective clinical confirmation of TB. The Low-risk population included 117 healthy blood donors from the French National Blood Bank.ExposuresTuberculosis.Main Outcomes and MeasuresPositive a...
The diverse clinical presentation of Helicobacter pylori (H. pylori) infection results from the i... more The diverse clinical presentation of Helicobacter pylori (H. pylori) infection results from the interaction between bacterial virulence, host genetics, socio-demographic and environmental factors. This study aimed to characterize Helicobacter pylori virulence genes and the associated behavioral factors among dyspeptic patients in Burkina Faso. Two hundred and fifty (250) stool samples were collected from patients with dyspepsia seen at health centers in Ouagadougou, Burkina Faso. Bacterial deoxyribonucleic acid (DNA) was extracted using a commercial kit. Virulence genes were detected using conventional multiplex Polymerase Chain Reaction with specific primers. The overall prevalence of Helicobacter pylori of the 250 participants was 91.20%. Ca-gA virulence gene was present among 20.19% of individuals, while babA2 and vacA were detected respectively among 9.65% and 67.54% of the population positive for Helicobacter pylori. Among vacA subtypes, vacAs1 was the most frequent, with 39.04%, followed by vacAi1 (19.74%), vacAi2 (17.54%), and vacAs2 with 10.96%. Regarding vacAm1 and vacAm2, they were less frequent at 6.14% each. "Handwashing three times or less per day" significantly increased the risk of having vacAi2 allele and H. pylori rRNA16s, with p-values of 0.013 and 0.020, respectively. The consumption of non-tap water increases the risk of carrying the cagA virulence gene. Additionally, H. pylori-positive
IP international journal of medical microbiology and tropical diseases, Apr 15, 2023
Abstract Background: This study aimed to evaluate the correlation between suspected COVID-19 symp... more Abstract Background: This study aimed to evaluate the correlation between suspected COVID-19 symptoms and RT-PCR results in the diagnosis of SARS-CoV-2 infection in Burkina Faso. Materials and Methods: We analyzed SARS-CoV-2 RT-PCR routine diagnostic data in Burkina Faso. Data were collected from March 9, 2020 to September 30, 2020 in the framework of the COVID-19 surveillance. Sensitivity, specificity, predictive values, and Kappa concordance were used to check the correlation between COVID-19 symptoms and the RT-PCR results. Results: A total of 2217 participants were tested for COVID-19 using RT-PCR, of them 779 COVID-19 positive. The mean age of the participants was 38.7± 17.69 years. Suspected symptoms presented by participants were fever (40.4%), cough (38.6%), asthenia (27.3%), headache (23.6%), dyspnea (20.8%), and odynophagia (16.3%). The sensitivity of presence of at least a clinical sign compared to RT-PCR results was 62.13% and the specificity was 39.85%. The kappa agreement between the presence of COVID-19 suspected symptoms and RT-PCR results was 0.017. The presence of aguesia and/or anosmia in patients induced a positive predictive value of RT-PCR of 91.30%. Conclusion: The correlation between the COVID-19 symptoms and RT-PCR results in the diagnosis of COVID-19 was very weak. The present study confirms that most clinical signs associated with SARS-CoV-2 infection are not specific to COVID-19, hence the need to always combine RT-PCR or other biological tests with the clinical diagnosis. However, aguesia and anosmia are of interest with a high degree of RT-PCR positivity when present in a COVID-19 suspected patient. Keywords: Diagnosis, Symptoms, Correlation, COVID-19, SARS-CoV-2, RT-PCR
Background The aim of this study was to evaluate the performance of ten (10) SARS-CoV-2 serologic... more Background The aim of this study was to evaluate the performance of ten (10) SARS-CoV-2 serological rapid diagnostic tests in comparison with the WANTAI SARS-CoV-2 Ab ELISA test in a laboratory setting. Materials and methods Ten (10) SARS-CoV-2 serological rapid diagnostic tests (RDTs) for SARS-CoV-2 IgG/IgM were evaluated with two (2) groups of plasma tested positive for one and negative for the other with the WANTAI SARS-CoV-2 Ab ELISA. The diagnostic performance of the SARS-CoV-2 serological RDTs and their agreement with the reference test were calculated with their 95% confidence intervals. Results The sensitivity of serological RDTs ranged from 27.39 to 61.67% and the specificity from 93.33 to 100% compared to WANTAI SARS-CoV-2 Ab ELISA test. Of all the tests, two tests (STANDARD Q COVID-19 IgM/IgG Combo SD BIOSENSOR and COVID-19 IgG/IgM Rapid Test (Zhejiang Orient Gene Biotech Co., Ltd)) had a sensitivity greater than 50%. In addition, all ten tests had specificity greater tha...
Selon l’organisation Mondiale de la Sante (oMS), l’afrique Sub-saharienne est l’une des regions ... more Selon l’organisation Mondiale de la Sante (oMS), l’afrique Sub-saharienne est l’une des regions les plus touchees par les infections sexuellement transmissibles (iST). au Burkina Faso, la prise en charge des infections genitales repose sur une approche syndromique, et les donnees sur leurs etiologies sont rares. Cette etude a pour objectif d’etablir le profil microbiologique des infections genitales basses chez patientes âgees d’au moins 15 ans dans la ville de Bobo-dioulasso. il s’est agi d’une transversale et prospective qui s’est deroulee d’octobre 2014 a Juillet 2015 dans le laboratoire d’analyse de biologie medicale eXalaB. Chez chaque femme consentante se presentant avec un bulletin d’examen, un prelevement cervico-vaginal a ete realise, examine a la microscopie, puis mis en culture pour l’identification des pathogenes. la susceptibilite aux antimicrobiens a ete determinee par la methode de diffusion sur gelose selon les recommandations(2013) du comite de l’antibiogra...
This study aimed to evaluate the diagnostic performance of commercially rapid point-of-care (POC)... more This study aimed to evaluate the diagnostic performance of commercially rapid point-of-care (POC) tests used for HCV antibodies detection. This is a case-control study conducted in Ouagadougou between December 2014 and January 2015. Three POC for HCV antibodies detection (SD Bioline HCV test®, Anti-HCV dipstick® and First response® HCV card test) marketed in Burkina Faso were evaluated. Architect anti-HCV assay and ImmunoComb® II HCV were combined and used as a reference test. All three tests were evaluated with a panel of 62 anti-HCV positive sera and 62 anti-HCV negative sera. The tests performance was calculated using the software OpenEpi. The three rapid POC tests had a specificity of 100% (95% CI: 94.17-100). However, the sensitivities were 33.87% (95% CI: 23.34-46.28) for the SD Bioline HCV test®, 41.94% (95% CI: 30.48-54.33%) for Anti-HCV dipstick® and 45.16% (95%CI: 33.42-57.47%) for First response® HCV card test. The tests evaluated in this study had good specificity but p...
Uploads
Papers by Tani Sagna