Papers by Titis Nurmasitoh
Open Access Macedonian Journal of Medical Sciences
BACKGROUND: Various types of Islamic fasting, apart from Ramadan fasting, have not been studied f... more BACKGROUND: Various types of Islamic fasting, apart from Ramadan fasting, have not been studied for their effects on health and metabolic processes. AIM: This study aimed to evaluate the effect of models of Ramadan, Dawood, and Monday-Thursday fasting on metabolic parameters in high-fat-high-fructose (HFHF)-induced rats. METHODS: Wistar rats were subjected to normal diet control, HFHF diet alone, and modeling Islamic fastings such as Ramadan, Dawood, and Monday-Thursday fasting models, within a period of 29 days at night that HFHF previously induced for 2 weeks. Serum lipid profile, glucose, uric acid, aspartate transaminase (AST) and alanine transaminase (ALT) for liver function, and urea and creatinine for kidney function were assessed after HFHF induction (pre-test) and after fasting treatment (post-test). RESULTS: HFHF administration for 2 weeks caused dyslipidemia and increased urea levels significantly. However, other parameters were impaired but not statistically significant....
Proceedings of the 1st International Conference on Community Health (ICCH 2019), 2020
Obesity is still a national problem. The prevalence of obesity is raising with increasing individ... more Obesity is still a national problem. The prevalence of obesity is raising with increasing individual income. Various innovations in the field of sports do not increase the desire and tendency to exercise. Personal motivation is needed to lose weight which is directly beneficial to health. The presence of stigma in obese women may increase the tendency to exercise. The purpose of this study was to investigate the desire to exercise in obese adult women. This report is the second report of the ongoing research entitled "The Effect of Obesity towards Low Physical Activity" funded by the Research and Community Service Directorate, General Directorate of Research Strengthening and Developing, Ministry of Research, Technology, and Higher Education, Indonesia. This study took data from 10 obese female subjects and 12 non-obese female subjects with age matching. Data was collected through a questionnaire and analyzed using the Mann Whitney test. Data analysis employed the Medcalc program and was declared significant with p <0.05. The analysis revealed that there was no difference in median sports motivation scores between groups (60.5 vs 62, p = 0.407). The results of this study indicate that stigma in obese women has no effect on the desire to exercise.
Deficiency of vitamin D in the world is high. Vitamin D deficiency has numerous negative effects.... more Deficiency of vitamin D in the world is high. Vitamin D deficiency has numerous negative effects. The purpose of this study was to determine the proportion of vitamin D deficiency in male adolescents in Yogyakarta Indonesia. This research is a cross-sectional study. The height, weight, physical activity, lipid profile laboratory data and vitamin D levels of subjects were recorded in the study. Body mass index (BMI) is calculated as body weight divided by height. Moderate exercise in physical is determined as enough activity for 150 minutes in week for moderate exercise and 75 minutes in week for strenuous exercise. Dyslipidaemia is diagnosed as an abnormal lipid level of three criteria: LowDensity Lipoprotein (LDL) ≥140 mg/dl, High-Density Lipoprotein (HDL) <40 mg/dl, and triglyceride ≥150 mg/dl. Vitamin deficiency was determined using the enzymes linked immunoassay method by measuring the activity of 25hydroxy vitamin D using serum samples of subjects. A total of 60 male adolesc...
Background : The high prevalence of hypertention and its complications made the society more awar... more Background : The high prevalence of hypertention and its complications made the society more awareness. . The people do some efforts to control blood pressure by limit the consumption of the food that could arise blood pressure such as mutton . The aim of this research was to determine the differences of average blood pressure before and after mutton consumption. Methods : This research was a part of experimental method. The research was conduct at Belang Wetan, Klaten Utara. The sample of this research were 42 people with inclusion and exclusion criteria. The samples are chosen by consecutive sampling method. The blood pressure measured before mutton consumption and after 60 minutes after consuming with sphigmomanometer . Results: The average of systolic blood pressure before and after consumption was 105.48 ± 10.41 113.76 ± 7.6 mmHg into (p = 0.00). While the average diastolic blood pressure before and after mutton consumption was 67.62 ± 7.9 to 75 ± 5.52 mm Hg (p = 0.0...
Open Access Macedonian Journal of Medical Sciences, 2021
BACKGROUND: Obesity occurs due to an imbalance between the calories and the energy released. On t... more BACKGROUND: Obesity occurs due to an imbalance between the calories and the energy released. On the animal model, obesity is considered as the ground for low physical activity. This is caused by low dopamine D2 receptor in the striatum. However, this suggestion is still unproven in the human condition. AIM: The aim of this study was to find out difference in dopamine expression in obese subjects compared to non-obese subjects when triggered by the stimuli of physical activity. METHODS: This is a quasi-experimental study. The sample was obese and non-obese (control) female who met inclusion and exclusion criteria. Before treatment was given, subjects were asked to fill out a depression, anxiety, and exercise motivation questionnaire. All subjects were tested for vital signs, anthropometrics, and neurological examinations to determine the initial condition. Then, the subjects saw video about physical activity and were taken for blood to measure blood dopamine levels using enzyme-linke...
Open Access Macedonian Journal of Medical Sciences, 2021
BACKGROUND: Alzheimer’s Dementia (AD) cases are increasing with the global elderly population. To... more BACKGROUND: Alzheimer’s Dementia (AD) cases are increasing with the global elderly population. To study the part of the brain affected by AD, animal models for hippocampal degeneration are still necessary to better understand AD pathogenesis and develop treatment and prevention measures. AIM: This study was a systematic review of toxic substance-induced animal models of AD using the Morris Water Maze method in determining hippocampal-related memory impairment. Our aim was reviewing the methods of AD induction using toxic substances in laboratory rodents and evaluating the report of the AD biomarkers reported in the models. METHODS: Data were obtained from articles in the PubMed database, then compiled, categorized, and analyzed. Eighty studies published in the past 5 years were included for analysis. RESULTS AND DISCUSSION: The most widely used method was intracerebroventricular injection of amyloid-β _substances. However, some less technically challenging techniques using oral or i...
Jurnal Kedokteran dan Kesehatan Indonesia, 2009
Background of choosing the subject: In recent years world society including Indonesia tend to con... more Background of choosing the subject: In recent years world society including Indonesia tend to consider the use of herbal medicine-back to nature. The advantages of herbal medicine become an interesting discussion. Most information expand in society is empirical rather than scientific evidence. Thus red betel vine's benefit also becomes interesting discussion but evidence based medicine about it is very little. The purpose of this study is to know the potency of antibacterial power of ethanol extract of red betel vine (Piper crocatum) toward gram positive and gram negative bacteria. Research Methodology: This research was laboratory experimental study. The extract of red betel vine was made with standard ethanol solvent. This extract was examined in Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 35218. Experimental method is serial dilution. Result was analyzed descriptively with Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The examination in each bacterium was repeated 5 times. Result: The result shown that ethanol extract of red betel vine (Piper crocatum) has ability to inhibit the growth of Gram positive (Staphylococcus aureus) and to kill it on concentration 25%. While the ability to inhibit the growth of Escherichia coli (Gram negative) on 6,25% (visual observation and after has been planted in Mc Conkey media). From result repetition in 5 times, gram negative is more stable rather than gram positive. Conclusion: The ethanol extract of red betel vine (Piper crocatum) has antibacterial effect toward Gram positive and gram negative. MIC and MBC Staphylococcus aureus at 25% while for MIC and MBC Escherichia coli at 6,25%.
Proceedings of the International Conference on Medical Education (ICME 2021), 2021
Introduction: The pandemic situation that has been taking place in Indonesia since March 2020 has... more Introduction: The pandemic situation that has been taking place in Indonesia since March 2020 has brought several changes to the learning process in medical faculty. Lab work is one of the online lessons that medical students have to complete. Online lab work carried out by students is expected to be able to achieve the learning goals. The purpose of this study was to determine students' perceptions of the implementation of online lab work. Methods: This research was an observational study with a cross-sectional design. The samples in this study were all students who participated in the physiology lab work in the nervous and musculoskeletal block. Data were obtained from the lab work evaluation questionnaire. Results: A total of 91.12% students have read the materials in the form of videos and guidebooks uploaded through Google Classroom. Students were satisfied with the implementation of lab work (60.95%) and considered the video in Google Classroom useful (98.22%). The percentage of students who agreed that online physiology lab work is important to be carried out synchronously reached 63.31%. Qualitative data showed that some students felt the online lab work helped understand the material. The problem with lab work was that online lab work was less comfortable because students could not practice directly and were constrained by internet signals. Conclusion: Students' perceptions of online physiology lab work in nervous and musculoskeletal block are considered important and support understanding of the block materials.
Universa Medicina, 2018
BackgroundShortening is widely used as raw material for bread and other foods. Hypercholesterolem... more BackgroundShortening is widely used as raw material for bread and other foods. Hypercholesterolemia increases aortic arch foam cell formation and abdominal aortic wall thickness. This study aimed to determine the effect of shortening on the number of aortic arch foam cells and abdominal aortic wall thickness in rats.MethodsThis study was of experimental posttest control group design. Twenty four male Wistar rats were randomized into 4 groups. The negative control group (C-) received standard feed, the positive control group (C+) standard high-fat feed, group T1 shortening and standard feed at a ratio of 1:5 and group T2 shortening and standard feed at a ratio of 1:10. The interventions were given for 6 weeks through gavage. The foam cell count in the aortic arch and the thickness of the abdominal aortic wall were measured. One-way ANOVA test was used to analyze the data. ResultsThere was no significant difference in the mean foam cell count of the aortic arch between the four groups...
Journal Of The Indonesian Medical Association, 2021
Pendahuluan: Salah satu metode untuk meningkatkan prestasi belajar adalah dengan metode peer tuto... more Pendahuluan: Salah satu metode untuk meningkatkan prestasi belajar adalah dengan metode peer tutoring. Mahasiswa dapat menumbuhkan dan meningkatkan minat belajar mereka, sehingga materi-materi pembelajaran pada tutorial dapat dengan mudah dipahami. Dengan meningkatnya minat belajar mahasiswa, maka keberhasilan belajar mahasiswa akan tercapai. Permasalahan yang diangkat dalam penelitian ini adalah bagaimana pengaruh metode peer tutoring terhadap hasil keaktifan nilai tutorial. Tujuan Penelitian yaitu mengetahui adanya hubungan peer tutoring terhadap nilai keaktifan tutorial Blok Pada Anak pada mahasiswa Fakultas Kedokteran UII Angkatan 2015.Metode: Penelitian yang dilakukan adalah penelitian eksperimental murni. Desain penelitian ini menggunakan posttest only with control design. Sejumlah 40 subjek penelitian berasal dari 2 kelompok berbeda. Perbandingan kelompok kontrol dengan kelompok perlakuan adalah 1:1. Kelompok kontrol adalah kelompok yang tidak diberikan peer tutoring dan kelo...
Universa Medicina, 2018
BackgroundChronic and degenerative diseases are closely related to modern lifestyles that tend to... more BackgroundChronic and degenerative diseases are closely related to modern lifestyles that tend to be deficient in physical activity but excessive in food intake. One method used to overcome this problem is dietary restriction through intermittent fasting. Intermittent fasting decreases the risk of chronic and degenerative diseases, e.g. by lowering oxidative stress. Oxidative stress can be determined from the malondialdehyde (MDA) levels and lipid profile in the blood. The present study aimed to determine the effect of intermittent fasting on plasma MDA levels and lipid profile of Wistar rats (Rattus norvegicus).MethodsThis research was a laboratory experimental research using a post test control group design. Twenty four male Wistar rats (Rattus norvegicus) were randomly assigned to 4 groups, ie control group (C), fasting group (F), non-fasting obese group (NFO), and fasting obese group (FO). Fasting treatment given in this research was intermittent fasting, comprising one day of f...
Universa Medicina, 2019
Background<br />Dopamine is an important neurotransmitter that plays a role in the pathogen... more Background<br />Dopamine is an important neurotransmitter that plays a role in the pathogenesis of anxiety and depression. Dopamine secretion occurs when there is a pleasurable stimulus. Blood dopamine levels have the potential to be developed as biomarkers of depression or anxiety, but previous studies related to the relationship between dopamine levels and levels of anxiety and depression are still controversial. The purpose of this study was to determine the correlation between anxiety and depression score with dopamine level in young adults. <br /><br />Methods<br />This was an observational cross sectional study. A total of 43 subjects aged 18 to 40 years were recruited by consecutive non-random sampling according to inclusion and exclusion criteria. Anxiety and depression were assessed using the Beck anxiety inventory and Beck depression inventory-II questionnaire. Dopamine plasma level was measured using the enzyme-linked immunosorbent assay (ELISA). T...
Bangladesh Journal of Medical Science, 2018
Background: Literature study shows, there are several variations regarding BCCAO duration and dur... more Background: Literature study shows, there are several variations regarding BCCAO duration and duration of reperfusion after BCCAO that can cause cerebral ischemia. Duration of BCCAO techniques varies between 10 to 30 minutes, while the duration of reperfusion period ranging between 45 minutes to 72 hours. Differences in the duration of occlusion, duration of BCCAO reperfusion and the sex of animal model could lead to different responses to ischemia conditions.Objective. This study aims to determine whether the duration BCCAO and sex of the animal models influences the volume of cerebral ischemia after 24 hours reperfusion.Method: This study uses post-test only study group design. The subjects are 20 female and 20 male Wistar rat that being divided into 8 groups which are male rat with occlusion duration of 5 minutes, 10 minutes and 20 minute, also the female rat with occlusion duration of 5 minutes, 10 minutes, 20 minutes respectively. BCCAO occlusion then followed by 24 hour reperf...
Drug and chemical toxicology, Jan 27, 2017
Monosodium glutamate-induced exitotoxicity causes oxidative stress in many brain areas, including... more Monosodium glutamate-induced exitotoxicity causes oxidative stress in many brain areas, including the medial prefrontal cortex. The ethanolic garlic (Allium sativum) extract is considered as a neuroprotective substance. The present study aimed at investigating the effects of the ethanolic fermented garlic extract on the working memory and the pyramidal cell number of the medial prefrontal cortex of adolescent male Wistar rats exposed to monosodium glutamate (MSG). Twenty-five rats were randomly divided into five groups. The C- group was given 0.9% NaCl solution. The C + group was given 2 mg/g bw of MSG. The T1, T2, and T3 groups were given MSG and garlic extract (0.0125, 0.025, and 0.05 mg/g bw, respectively). All treatments were conducted for 10 days. The working memory capability of the rats was measured using Y-maze test. The total number of pyramidal cells of the medial prefrontal cortex was estimated using physical fractionator method. The working memory performances of the T1,...
Universa Medicina, 2016
Background<br />There are people in Yogyakarta, who use black sugarcane decoction (BSD) to ... more Background<br />There are people in Yogyakarta, who use black sugarcane decoction (BSD) to prevent stroke. BSD contains policosanol and antioxidants. It has been proven that policosanol can reduce global ischemia in Mongolian gerbils. This study aims to evaluate the effect of BSD on brain ischemia in a rat stroke model. <br /><br…
Universa Medicina, 2016
Background<br />Coronary heart disease (CHD) is a major cause of morbidity and mortality th... more Background<br />Coronary heart disease (CHD) is a major cause of morbidity and mortality throughout the world, including Indonesia. One of the risk factors for CHD is hypercholesterolemia. One of the natural products that has been developed for the treatment of hypercholesterolemia is honey. Honey contains fructooligosaccharides, various vitamins, minerals, and enzymes which are supposedly able to lower blood cholesterol levels. This research aimed to study the influence of honey on the levels of blood total cholesterol, triglyceride, and low density lipoprotein (LDL) levels in Wistar rats. <br /><br…
Jurnal Kedokteran dan Kesehatan Indonesia, 2015
Every systems in human body correlate to maintain homeostasis. One of those systems which contrib... more Every systems in human body correlate to maintain homeostasis. One of those systems which contribute to maintain homeostasis is the immune system. The immune system defends physiological functions against foreign substances and cancer cells through a complex and multilayered mechanism. The ability to defend against foreign substances and abnormal cells is done by two types of immune system, which are Innate immune system and adaptive/acquired immune system. There are also certain factors that affect the immune system, for instance physiological factor, nutrition, psychological factor, environental factor, and exercise or physical activities. Regular exercises and physical activities with moderate intensity will boost the immune system. Sedentary lifestyle and minimal physical activity will increase the risk of infectious diseases. While exercises and physical activity with high intensity will suppress the immune system hence increase the risk of infection. Sistem-sistem dalam tubuh manusia bekerja sama untuk mempertahankan homeostasis. Salah satu sistem yang berkontribusi mempertahankan homeostasis adalah sistem imun. Sistem imun mempertahankan fungsi tubuh terhadap invasi sel asing dan sel kanker melalui mekanisme pertahanan yang kompleks dan berlapis-lapis. Kemampuan untuk mengatasi substansi asing dan sel abnormal yang membahayakan dilakukan oleh kerja sama dua tipe sistem imun, yaitu sistem imun non-spesifik atau innate immune system dan sistem imun spesifik atau adaptive/acquires immune system. Terdapat faktor-faktor tertentu yang mempengaruhi status imun, antara lain faktor fisiologis, nutrisi, psikologis, lingkungan, dan kebiasaan berolah raga atau beraktivitas fisik. Olah raga dan aktivitas fisik dengan intensitas moderat secara teratur akan meningkatkan sistem imun. Kebiasaan hidup sedenterian dan aktivitas fisik yang minimal akan meningkatkan risiko terkena penyakit infeksi. Selain itu, olah raga dengan intensitas berat dan sangat berat justru menekan sistem imun sehingga meningkatkan risiko terkena infeksi
Jurnal Kedokteran dan Kesehatan Indonesia, 2015
Background: Ethanol is one types of alcohol used in beverages. Excessive use of ethanol causes da... more Background: Ethanol is one types of alcohol used in beverages. Excessive use of ethanol causes damage to organs, especially in the liver. Liver, which is an organ in the body, serves as the metabolism of various substances including ethanol. Liver damage that occurs can be known with one of these liver function examination, which check the levels of SGPT and SGOT. Objective: This research aims to look for the difference of SGPT's and SGOT's levels between ethanol multistep doses group and control group in Wistar rats (Rattus norvegicus) SGPT and SGOT levels. Methods: This study used an experimental research design with post test only control group design. The sample consisted of 21 male Wistar rats, which were divided into 3 groups. Each group consisted of 7 rats. The first group (K1) was the control group, which was given aquades. The second group (K2) were given ethanol 20% as 2 g/kg pe roral. And the third group (K3) were given with 25% ethanol as 2.5g/kg peroral. The treatment was given for 4 weeks. At the 31st day blood was taken from orbitalis sinus as much as 1 ml for check the level of SGPT and SGOT. Results: The results are the average increase of SGPT and SGOT levels in K2 compared to the K1. While on K3 showed that the average levels of SGPT and SGOT were lower than K1. SGPT levels showed a significant difference (p<0.05) in all treatment groups in comparison with the control group. The value of SGOT did not show a significant difference (p>0.05). Conclusion: There is significant differences in the levels of SGPT treatment group in comparison with the control group, but there is no significant differences in the level of SGOT. Latar Belakang: Etanol merupakan sebutan untuk alkohol yang digunakan dalam minuman. Penggunaan etanol yang berlebihan akan menyebabkan kerusakan pada organ tubuh, terutama hepar. Hepar berfungsi sebagai tempat metabolisme berbagai zat termasuk etanol. Kerusakan hepar yang terjadi dapat diketahui salah satunya dengan pemeriksaan fungsi hepar, dengan memeriksa kadar SGPT dan SGOT. Tujuan: Penelitian ini bertujuan untuk melihat apakah terdapat perbedaan kadar SGPT dan SGOT antar kelompok yang diberi etanol dosis bertingkat dibandingkan dengan kontrol pada tikus galur Wistar (Rattus norvegicus).
Jurnal kedokteran dan kesehatan Indonesia, 2015
Proses penuaan yang terjadi pada lansia mengakibatkan perubahan pada sistem organ, terutama kulit... more Proses penuaan yang terjadi pada lansia mengakibatkan perubahan pada sistem organ, terutama kulit yang dapat mengalami penurunan fungsi sensoris. Fungsi sensoris dapat dideteksi secara sederhana menggunakan tes diskriminasi dua titik. Sementara itu, pemakaian pelembap dapat memperbaiki tampilan dan fungsi kulit pada lansia. Tujuan Untuk mengetahui perbedaan hasil tes diskriminasi dua titik pada lansia di Panti Wredha Abiyoso Yogyakarta sebelum dan sesudah pemberian pelembap. Metode Penelitian ini merupakan penelitian eksperimen yang menggunakan rancangan pre-test and post-test control group dan melibatkan 32 subjek penelitian yang terbagi pada kelompok kontrol dan kelompok eksperimen. Hasil Analisis dengan uji T tidak berpasangan menunjukkan bahwa secara statistik terdapat perbedaan hasil tes diskriminasi dua titik yang signifikan antara kelompok kontrol dan kelompok eksperimen (p=0,001). Pada kelompok eksperimen, terdapat perbedaan nilai pre-test dan posttest yang bermakna setelah pemberian pelembap (p=0,007), sedangkan pada kelompok kontrol tidak terdapat perbedaan nilai yang signifikan antara hasil pre-test dan post-test (p=0,344). Kesimpulan Terdapat perbedaan hasil tes diskriminasi dua titik yang bermakna pada lansia di Panti Wredha Abiyoso Yogyakarta antara kelompok kontrol dan kelompok eksperimen.
Journal of Pharmacy and Nutrition Sciences, 2018
Background:A diet high in carbs and fat puts people at risk of obesity. Obesity causes changes in... more Background:A diet high in carbs and fat puts people at risk of obesity. Obesity causes changes in the immune system and increases the risk of premature ageing, including in the lymphoid organs ― such as the thymus and spleen. Fasting is expected to improve the immune system. The purpose of this research is to determine the effects of intermittent fasting on images of the structure of the thymus, the number of fat cells, Hassall’s corpuscles of the thymus, the area and density of pulp white spleen, and the number of leukocytes in Wistar rats (Rattus norvegicus) fed a diet high in carbs and fat. Methods:An experimental study with post-test only control group design, with 15 male rat subjects aged 2.5 months were divided into three groups: first group had a diet that was ad libitum (AL); second group were given a diet high in fats and carbohydrates for 1 month then were fed ad libitum (HCL); and third group were given a diet high in fat and carbohydrates for 1 month continued with alte...
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Papers by Titis Nurmasitoh