Türk Ortopedi ve Travmatoloji Birliği Derneği dergisi, 2017
Türk Ortopedi ve Travmatoloji Birliği Derneği gerekir. Trombositin yaşlanmasıyla birlikte ortaya ... more Türk Ortopedi ve Travmatoloji Birliği Derneği gerekir. Trombositin yaşlanmasıyla birlikte ortaya çıkan "desializasyon" yani sialik asidin kaybı, hücrenin dolaşımdan uzaklaştırılması için bir sinyal olarak algılanır ve retiküloendoteliyal sistem tarafından dolaşımdan uzaklaştırılır. Hücrelerin en önemli elemanları kuşkusuz proteinlerdir. Her bir hücrenin üstlendiği görevine göre yapısında yer alan protein profili farklıdır ve bu proteinlerin hepsine, o hücrenin proteom'u adı verilir. Trombositlerde nükleus olmadığı için, protein profilinin çok sınırlı ve statik olduğu düşünülebilir. Ancak, megakaryositlerden kendilerine miras kalmış olan proteinler ve intrasitoplazmik mRNA'lar incelendiğinde, trombositlerin çok zengin bir protein profiline sahip olduğu görülür. Sonuç olarak, trombosit protein profilinin kaynağı megakaryositler ve plazmadır. Megakaryositler, kaynağını pluripotent kök hücrelerden alan, hücre bölünmesi olmaksızın çok sayıda DNA replikasyonu (kopyalanarak çoğalma) ile matür (olgun) hale dönüşen sıra dışı hücrelerdir. Asıl önemli rolleri, dolaşıma trombositleri salan çok özgün hücreler olmalarıdır. İnsan bedeni, her gün yaklaşık 100 milyar trombosit yapar. Bunların çok az bir kısmı hemostazda kullanılır; geriye kalan trombositler karaciğer ve dalak sayesinde dolaşımdan uzaklaştırılır. Trombositlerin dolaşımdaki yaşam süreleri yaklaşık 10 gündür. Trombositlerin dolaşımdan uzaklaştırılmalarında (klirensinde), membran proteinlerinin karbonhidrat modifikasyonları oldukça önemlidir. Özellikle membran proteinlerinin glikozillenmiş kısımlarının, sialik asit başlığıyla korunması
In this study, we aimed to evaluate and compare the effects of retrograde intrarenal surgery (RIR... more In this study, we aimed to evaluate and compare the effects of retrograde intrarenal surgery (RIRS) and schok wave lithoripsy (SWL) interventions on renal functions by analyzing glomerular filtration rate (GFR) values. Materials and Methods: A total of 95 patients, who underwent RIRS or SWL in 2017 at Gülhane Training and Research Hospital, were included in this retrospective study. Forty-six of these patients (48.4%) were in RIRS group and 49 (51.6%) were in SWL group. Preoperative, early-postoperative (on the first postoperative day) and late-postoperative (on the first postoperative month) GFR values were calculated using the "abbreviated Modification of Diet in Renal Disease" Method. Changes in GFR values after RIRS and SWL treatments were compared statistically. Results: When the early postoperative and late postoperative GFR levels were compared, a statistically significant difference was observed between the groups (p=0.04 and p<0.001, respectively). For RIRS group, there was a 0.37±13.5 mL/min/1.73 m² increase and for SWL group, there was 5.65±12.5 mL/min/1.73 m² increase in GFR values in the early postoperative period. There was a 2.40±14.1 mL/min/1.73 m² decrease in RIRS group and 7.75±11.8 mL/min/1.73 m² increase in SWL group in GFR values in the late postoperative period. In general linear model, there was a statistically significant difference in changes in GFR over time between RIRS and SWL groups (p=0.002). There was also a statistically significant difference when the changes in GFR over time were compared according to stone locations (p=0.02). Conclusion: RIRS is associated with less improved GFR in comparison with SWL. SWL should be considered as first line treatment for kidney and ureteral stones when considering the changes in GFR values comparing to RIRS especially for stones smaller than 20 mm.
Intestinal ischemia-reperfusion injury (II/R) is a condition with significant morbidity and morta... more Intestinal ischemia-reperfusion injury (II/R) is a condition with significant morbidity and mortality. In this study, we aimed to demonstrate the protective effect of carnosol, an antioxidant, anti-inflammatory, anti-carcinogenic and anti-microbial agent, on pro-inflammatory cytokines, tissue angiopoietins, histopathological damage and bacterial translocation in rats subjected to II/R injury. Thirty male Sprague-Dawley rats were divided into 3 groups: control group, intestinal ischemia/reperfusion group (II/R) and carnosol group (II/R+C). Ischemiareperfusion injury was conducted by laparotomy. All rats received 10 10 colony forming units/milliliters E.coli 12 hours prior to surgery. II/R+C rats received 3 mg/kg/day carnosol per oral starting from 3 days prior to surgery. After 72 hours of post-surgical follow-up, rats were IAAOJ | Health Sciences | 2019 / 5 (2) 106 re-laparotomized for specimen collection. II/R+C group had lower serum TNF-α, IL-1β and IL-6 levels when compared with II/R group (p=0.026, 0.006 and 0.045, respectively). II/R group had significantly higher tissue injury when compared with II/R+C group (p<0.001). II/R+C group rats had significantly reduced liver, spleen and mesenteric lymph node culture growths when compared with II/R group. Angiopoietin I and II levels were similar between groups. In conclusion, carnosol is a powerful anti-inflammatory and anti-microbial agent in II/R injury model. We showed effective reduction of pro-inflammatory cytokine profile, improvement of histopathological damage, and prevention of bacterial translocation.
Increased bone turnover is a hallmark of hyperthyroidism. The underlying factors of how thyroid h... more Increased bone turnover is a hallmark of hyperthyroidism. The underlying factors of how thyroid hormones affect bone cells are still under the spotlight. Previous studies indicated serum osteoprotegerin (OPG), receptor activator of NF-kB ligand (RANKL), and interleukin-6 (IL-6) as mediators of the effect of thyroid hormones on bone metabolism. Ultimately, the present research aimed to examine the association of IL-6 with OPG and RANKL in patients with hyperthyroidism. We carried out this study with 39 newly diagnosed and untreated Graves' patients and 43 healthy controls. In addition to routine tests, we measured serum OPG, RANKL, and IL-6 levels. Results: Mean age and sex distribution were similar in both groups. The hyperthyroid group had significantly higher OPG (p = 0.002) and IL-6 (p < 0.001) levels, but RANKL levels were significantly lower in this group (p < 0.001). We found OPG not to correlate with free T4 and T3, while it had a moderate and negative correlation with thyrotropin (TSH) (r = -0.372, p = 0.001). IL-6 had no correlation with OPG but positively correlated with free T4 (r = 0.445, p < 0.001) and free T3 (r = 0.326, p = 0.035). It also negatively correlated with RANKL (r = -0.247, p = 0.033). Maintaining skeletal development and integrity is partially regulated by a normal balance of thyroid hormones. We concluded that increases in serum OPG and IL-6 levels accompanied hyperthyroidism. However, excessive levels of the hormones might cause drops in serum RANKL levels. Our results suggested that OPG, RANKL, and IL-6 might be involved in the cross-talking among immunity, thyroid function, and bone metabolism in the case of hyperthyroidism.
Anjiyogenez, yeni kapiller oluşumu ile seyreden biyolojik bir süreçtir ve bazı fizyolojik ve pato... more Anjiyogenez, yeni kapiller oluşumu ile seyreden biyolojik bir süreçtir ve bazı fizyolojik ve patolojik durumlarda ortaya çıkmaktadır. Anjiyogenez pozitif ve negatif düzenleyici aktiviteye sahip moleküller arasındaki net dengeyle kontrol edilir. İnsan sağlığı açısından yeni damar oluşumu (anjiyogenez) ve kan damarlarının yayılımının kontrolü (anti-anjiyogenez) oldukça önemlidir. Bu nedenle, anjiyogenez aktif bir araştırma alanıdır. İn vitro modeller, bu süreci tarif etmede faydalı olsalar da, in vivo ne olduğunu yansıtmayabilir. Anjiyogenez araştırmalarında çok yaygın olarak kullanılan, yoğun vaskülarize civciv embriyo koriyoallantoik membran modeli, faydalı bir in vivo sistemdir. Civciv koriyoallantoik membran ölçümünün anjiyojenik yanıt açısından fiyat-etkin, basit, güvenilir ve tekrarlanabilir olması, araştırmacılar için çekici özellik taşımaktadır.
Background. Behçet's disease (BD) is a chronic inflammatory vasculitis affecting multiple organs.... more Background. Behçet's disease (BD) is a chronic inflammatory vasculitis affecting multiple organs. Uveitis is frequently seen in patients with BD, especially in Turkish population. To investigate vascular endothelial growth factor (VEGF) gene polymorphisms along with the levels of VEGF and VEGF receptors in patients with Behçet's uveitis (BU). Fifty-five BD-associated uveitis patients and 30 age-and sex-matched controls were included in this case-control study. The genotypes of the single nucleotide poymorphisms (SNPs): rs2010963 (+405G), rs3025039 (+936T) and rs699947 (-2598A) of the VEGF-A gene were determined using real-time polymerase chain reaction (RT-PCR) and serum levels of VEGF and VEGF receptors were measured using enzyme-linked immunosorbent assay (ELISA). No associations of the VEGF gene polymorphisms were observed in BD uveitis patients, but arthritis was present in 53.3% of patients not possessing CT genotype in C3025039→T polymorphism (p = 0.024). Although there were no statistically significant differences in serum VEGF-A, VEGF-C and soluble vascular endothelial growth factor receptor-3 (sVEGFR-3) levels (p < 0.05), serum vascular endothelial growth factor receptor-1 (VEGFR-1) and sVEGFR-3 levels were significantly higher in the BD group (p < 0.001 and p = 0.001, respectively). In addition, VEGF-C/soluble vascular endothelial growth factor receptor-2 (sVEGFR-2) ratio was significantly higher (p < 0.001), while VEGF-A/VEGFR-1 and VEGF-C/sVEGFR-3 ratios were significantly lower (p < 0.001 and p = 0.033, respectively) in BD patients compared to controls. Also, VEGF-C/sVEGFR-3 (p = 0.024, r = 0.37) and VEGF-C/sVEGFR-2 (p = 0.020, r = 0.38) ratios were positively correlated with disease duration. Conclusions. The significant changes in sVEGFR-3 levels and VEGF-C/sVEGFR-3 ratio has shown that lymphangiogenesis processes might take place in the pathogenesis of BD uveitis, and these parameters can be important indicators of evaluation of BD patients with uveitis together with disease duration.
Introduction: Angiogenesis (neovascularization), which means new vessel construction, is normal a... more Introduction: Angiogenesis (neovascularization), which means new vessel construction, is normal and physiologically, wound healing, embryogenesis, a necessary menstrual cycle it's a mechanism. When taken in appropriate amounts together with or separately with nutrients, mucosal and by regulating systemic immunity, ensuring nutritional and microbial balance in the intestines living nonpatogenic microorganisms that positively affect the health of the host it is called "probiotics". Lactic acid bacteria, the most probiotic microorganisms it constitutes its important group. Where probiotics have an effect on angiogenesis, and it is thought to help heal wounds through the road. With this research indicated that roles of Lactobacillus acidophilus and Lactobacillus rhamnosus on angiogenesis if present to demonstrate in vitro methods and the gene expression responsible for the formation of these effects it is intended to reveal. Material and Method: This study is an experiment...
Intestinal iskemi-reperfuzyon hasari (II/R), onemli morbidite ve mortaliteye sahip bir durumdur. ... more Intestinal iskemi-reperfuzyon hasari (II/R), onemli morbidite ve mortaliteye sahip bir durumdur. Bu calismada, anti-oksidan, anti-enflamatuvar, anti-karsinojenik ve anti-mikrobiyal bir ajan olan karnosolun II/R modeli uygulanan sicanlarda pro-inflamatuar sitokinler, doku anjiopoietinleri, histopatolojik hasar ve bakteriyel translokasyon uzerindeki koruyucu etkisini gostermek amaclanmistir. Otuz erkek Sprague-Dawley sicani 3 gruba ayrildi: kontrol grubu, intestinal iskemi/reperfuzyon grubu (II/R) ve karnosol grubu (II/ R+C). Iskemi-reperfuzyon hasari laparotomi ile saglandi. Tum sicanlara ameliyattan 12 saat once 1010 koloni olusturan birim/mililitre E.coli uygulandi. II/R+C grubuna, ameliyattan 3 gun once baslayarak oral 3 mg/kg/gun karnosol uygulandi. Ameliyat sonrasi takipten 72 saat sonra, sicanlar ornek toplama icin yeniden laparotomize edildi. II/R+C grubunun II/R grubuna gore serum TNF-α, IL-1β ve IL-6 duzeyleri anlamli derecede azalmisti (sirasiyla p=0.026, 0.006 ve 0.045). I...
PurposeLactose intolerance is lactose digestive disorder due to lactase enzyme deficiency. This c... more PurposeLactose intolerance is lactose digestive disorder due to lactase enzyme deficiency. This can affect the quality of life by restricting the intake of certain foods. The aim of this study is to show the lactose intolerance to the restriction in food intake and quality of life.Design/methodology/approachThis study was conducted with adults aged 18–60 years. A survey was used to gather information on the demographic characteristics of the patients and their symptoms related to lactose intolerance. In addition, the Visual Analog Scale was administered to identify common symptoms and the World Health Organization Quality of Life (WHOQOL)-Bref Quality of Life Scale to determine their quality of life.FindingsThe average quality of life subscale scores was 56.25 ± 14.06 for physical, 58.29 ± 11.72 for mental, 63.28 ± 21.35 for social and 62.36 ± 16.37 for environmental. When VAS scores obtained for the common digestive system symptoms in lactose intolerance were compared with Quality ...
To evaluate the stone composition and the distribution of the stone types in the geographical reg... more To evaluate the stone composition and the distribution of the stone types in the geographical regions of Turkey according to where patients were born and where patients live. The patients who were treated for urinary system stone disease in our hospital between January 2011 and March 2019 were included in the study. Stone samples were collected by endoscopic stone surgery methods or shock wave lithotripsy. Stone analyses were made with manual chemical analysis methods. Stone types and distribution were recorded according to geographical regions of Turkey. Results: A total of 706 patients who were treated for urolithiasis were included in the study. Calcium oxalate (CO) stones were at the highest rates for both genders [127 (68.6%) and 359 (68.9%), for females and males, respectively]. There were no statistically significant differences in terms of stone types between genders (p=0.323). There were statistically significant more CO (297), uric acid (17), mixed stones (78) in the Anatolian (central) region than the other regions according to the geographical region where patients were born (p<0.001, for each). Additionally, there were significantly more calcium phosphate stone formers (25) in the Anatolian region according to the geographical region where patients lived (p<0.001). Conclusions: CO is the most common stone type in Turkey independently of where patients were born or where patients live. Higher prevalence rates of stone disease and CO stones were seen in Central Anatolia. Both geographic origin and dietary habits affect the incidence of urinary system stone disease.
Background and Objective: The 5-hydroxytriptamine type 7 receptors (5-HT7Rs) belong to the seroto... more Background and Objective: The 5-hydroxytriptamine type 7 receptors (5-HT7Rs) belong to the serotonin receptor family and modulate many processes in organism and are also found in membrane of endothelial cells (ECs). The LP-211 is a selective agonist of 5-HT7Rs. The purpose of this study was to investigate the effects of activated 5-HT7Rs by LP-211 on regulation of angiogenesis. The effect of LP-211 was tested in vitro by cell proliferation and matrigel assays and in vivo by chick chorioallantoic membrane (CAM) assay. Results: Cell proliferation assay showed that LP-211 had a positive effect on EC proliferation. The LP-211 activated migration of ECs and promoted angiogenesis in matrigel assay in vitro. Stimulation of 5-HT7R by LP-211 caused a significant increase in angiogenic response compared to the control group in CAM assay in vivo. The LP-211, selective agonist of 5-HT7R, was found to have an angiogenic effect in vitro and in vivo. Conclusion: The 5-HT7Rs can be considered to have an important role in EC functions and angiogenesis. Thus, 5-HT7Rs may be a potential target for the therapeutic interventions aimed to regulate the process of angiogenesis in diseases such as varicose veins, peripheral vascular diseases or vasculature of solid tumors. The LP-211 may be a candidate to improve the ineffective circulation in related disorders.
Probiyotikler, gastrointestinal sistem hastalıklarından depresyona kadar çok sayıda hastalık grub... more Probiyotikler, gastrointestinal sistem hastalıklarından depresyona kadar çok sayıda hastalık grubunda çalışılmış ve bu hastalıklar üzerinde probiyotiklerin olumlu etkiler gösterebildiği bildirilmiştir. Probiyotiklerin bildirilen olumlu etkilerini, hastalık gelişimi ve tedavi sürecinde canlı mikrobiyotasını zenginleştirerek gerçekleştirdiği düşünülmektedir. Yara iyileşmesi ise birçok farklı mekanizmanın etkisi altında bulunan bir süreçtir. Bakterilerin bu süreçte faydalı etkileri olduğu gibi farklı bakteri türlerine bağlı olarak olumsuz etkileri de olabilmektedir. Son yapılan çalışmalarda bazı probiyotik bakteri türlerinin topikal uygulaması ya da sistemik etkileri ile yara iyileşmesi üzerine etkili olduğu saptanmıştır. Özellikle gastrik ülserler üzerinde yapılan çalışmalarda probiyotik bakterilerin antibiyotikler ile karşılaştırıldığında, antibiyotik direncinin olduğu durumlarda anjiyogenezanjiyogenez üzerinden etki ederek daha etkili sonuçlar gösterdiği belirtilmiştir. Probiyotikle...
Introduction: Behçet's disease is a chronic inflammatory vasculitis presenting with immunological... more Introduction: Behçet's disease is a chronic inflammatory vasculitis presenting with immunological and endothelial changes. The aim of the present study is to evaluate blood levels of diagnostic markers which can be used in Behçet's patients with vascular involvement. Material and methods: Fifty Behçet's patients (22 with vascular involvement) and 30 healthy controls were included in the study. High-sensitivity C-reactive protein (hsCRP), erythrocyte sedimentation rate (ESR), tumor necrosis factor-α (TNF-α), apolipoprotein A1 (apoA1), apolipoprotein B (apoB), HDL, LDL, triglyceride, total cholesterol, Lp-plA-2, homocysteine and ischemia modified albumin (IMA) levels were analyzed. Statistical analysis was performed with the SPSS program version 11.0. p < 0.05 was accepted as statistically significant. Results: hsCRP, TNF-α, homocysteine, IMA, apoA1, apoB, HDL, Lp-pla2 and ESR levels in patient and control groups were significantly different (p < 0.001, p = 0.001, p < 0.001, p < 0.001, p = 0.005, p < 0.001, p < 0.001, p < 0.001 and p = 0.003 respectively). In Behçet's patients with vascular involvement, homocysteine, TNF-α and Lp-pla2 levels were significantly higher than in Behçet's patients without vascular involvement (p = 0.035, p = 0.010 and p < 0.001 respectively). Conclusions: Increased levels of inflammatory and atherogenic markers in Behçet's patients are an expected outcome due to the inflammatory nature of the disease. Especially, elevated levels of homocysteine, TNF-α and Lp-pla2 make them candidate diagnostic tools to be helpful in clinical evaluation of Behçet's disease patients with vascular involvement.
BACKGROUND/OBJECTIVES: Available data suggest that seasonal changes may influence the nutritional... more BACKGROUND/OBJECTIVES: Available data suggest that seasonal changes may influence the nutritional status and overall health of elderly individuals. Therefore, this study was conducted to investigate the effects of seasonal changes and related factors on energy and nutrient intake of older adults. SUBJECTS/METHODS: Individuals aged 65 years or over were prospectively enrolled in this single-center study (male: 11, female: 20). Data were collected between May 2013 and February 2014 during winter, spring, summer and autumn. Food consumption and biochemical parameters were taken during each season to assess the seasonal nutrition status of the elderly. Upon analysis of biochemical parameters (retinol, vitamin D and vitamin C), an high-performance liquid chromatography device was utilized whereas an Immulite 2000 device was utilized during analysis of serum folic acid and parathyroid hormone. RESULTS: Fruit, fat, egg and bread consumption varied seasonally in males and females (P < 0.05). During winter, daily energy intake was found to be greater than in other seasons in males (557 kcal) and females (330 kcal) (P < 0.05). Additionally, carbohydrates, vegetable protein, n-3 fatty acid and sodium intake increased in winter, while the n-6/n-3 ratio increased in summer among males (P < 0.05). Dietary fiber and sodium intake in winter, vitamin C, iron and zinc intake in spring, and cholesterol, retinol, vitamin D and niacin intake in autumn were found to be higher in females when compared to other seasons (P < 0.05). Serum parathyroid hormone level was higher in winter, and vitamin D level was higher in autumn in both genders (P < 0.05). In males, blood folic acid level was higher in winter, while vitamin C level was higher in females, and there was no seasonal variation in retinol concentration (P < 0.05). CONCLUSION: Food consumption and biochemical parameters showed significant seasonal variations in older adults. It is not clear if nutrition plans in older adults will benefit from consideration of seasonal changes in eating habits.
Diabetes mellitus (DM) is a chronic metabolic disease that requires continuous medical care, wher... more Diabetes mellitus (DM) is a chronic metabolic disease that requires continuous medical care, where the organism cannot adequately benefit from carbohydrates, fats, and proteins due to inherited and/or acquired deficiency in the production of insulin by the β-cells of the pancreas, the ineffectiveness of the produced insulin, or both (1). According to the International Diabetes Federation (IDF) Atlas in 2017, there are about 425 million people with DM, of which one-third are people older than 65 years (atlas.html). According to the World Health Organization (WHO), the number of people with DM increased from 108 million to 422 million between 1980 and 2014 (). Additionally, according to the IDF, over 6.694 million individuals suffer from DM in Turkey and the number of deaths due to DM is approximately 46,300 (2). If left untreated, DM can lead to varying degrees of disability and long-term complications that are not only major health issues but also can generate a number of social and economic problems. Around the world, up to 80% of deaths in patients with DM are closely associated with vascular disease, and in a study evaluating management of DM and DM policies in Turkey, cardiovascular complications were found to account for 32.6% of the total costs of DM, followed by 25% for nephrological complications, 6.4% for eye complications, and 6% for neurological complications (3). Angiostatin, a 38-kDa protein naturally occurring by fragmentation of plasminogen, is a potent endogenous inhibitor of proliferation of endothelial cells and angiogenesis (4). Although its mechanism of action has not yet been completely elucidated, there are many studies in the literature that have focused on the enhancing effect of angiostatin on endothelial cell apoptosis (5-7). The binding of angiostatin to plasma membrane-localized ATP synthetase suppresses the endothelial surface's ATP metabolism and thus downregulates endothelial cell proliferation and migration (8). Background/aim: The clinical effect of angiostatin in diabetes mellitus (DM) patients receiving insulin is a meaningful gap in the literature. In this study, we aimed to show the levels and the clinical significance of angiostatin in DM patients receiving insulin. This is a case-control study. Serum angiostatin levels were determined by ELISA. A total of 83 people consisting of healthy subjects (n = 36) and patients with a diagnosis of DM receiving insulin therapy (n = 47) were included in this study. The mean angiostatin levels of the DM group were significantly higher than those of the control group (86.0 ± 68.1 ng/mL and 58.0 ± 22.4 ng/mL, respectively; P = 0.011). Significantly lower angiostatin levels were determined in the DM patients receiving metformin with respect to those not receiving metformin (97.2 ± 74.4 ng/mL and 49.3 ± 7.0 ng/mL, respectively; P = 0.021). Significantly higher levels of angiostatin were observed among the DM patients using a beta-blocker (BB) than the DM patients not using a BB (115.5 ± 78.71 ng/mL and 73.44 ± 60.08 ng/mL, respectively; p = 0.029). This is the first study evaluating and demonstrating the serum angiostatin levels in DM patients receiving insulin. Further studies are required to understand the effect of angiostatin in diabetics and the effect of medications on angiogenesis in these patients.
Objectives. High morbidity and mortality rates due to chronic heart diseases in patients on hemod... more Objectives. High morbidity and mortality rates due to chronic heart diseases in patients on hemodialysis is a well-known entity. The main lipid disorder leading to atherosclerosis in these patients is LDL-cholesterol (LDL-C) elevation. Recently, it has been suggested that a decrease in ...
Angiogenesis is the process of generating new blood vessels from preexisting vessels and is consi... more Angiogenesis is the process of generating new blood vessels from preexisting vessels and is considered essential in many pathological conditions. The purpose of the present study is to evaluate the effect of aspartame on angiogenesis in vivo chick chorioallantoic membrane (CAM) and wound-healing models as well as in vitro 2,3-bis-2 H-tetrazolium-5-carboxanilide (XTT) and tube formation assays. In CAM assay, aspartame increased angiogenesis in a concentration-dependent manner. Compared with the control group, aspartame has significantly increased vessel proliferation ( p < 0.001). In addition, in vivo rat model of skin wound-healing study showed that aspartame group had better healing than control group, and this was statistically significant at p < 0.05. There was a slight proliferative effect of aspartame on human umbilical vein endothelial cells on XTT assay in vitro, but it was not statistically significant; and there was no antiangiogenic effect of aspartame on tube format...
Anjiyogenez, yeni kapiller oluşumu ile seyreden biyolojik bir süreçtir ve bazı fizyolojik ve pato... more Anjiyogenez, yeni kapiller oluşumu ile seyreden biyolojik bir süreçtir ve bazı fizyolojik ve patolojik durumlarda ortaya çıkmaktadır. Anjiyogenez pozitif ve negatif düzenleyici aktiviteye sahip moleküller arasındaki net dengeyle kontrol edilir. İnsan sağlığı açısından yeni damar oluşumu (anjiyogenez) ve kan damarlarının yayılımının kontrolü (anti-anjiyogenez) oldukça önemlidir. Bu nedenle, anjiyogenez aktif bir araştırma alanıdır. İn vitro modeller, bu süreci tarif etmede faydalı olsalar da, in vivo ne olduğunu yansıtmayabilir. Anjiyogenez araştırmalarında çok yaygın olarak kullanılan, yoğun vaskülarize civciv embriyo koriyoallantoik membran modeli, faydalı bir in vivo sistemdir. Civciv koriyoallantoik membran ölçümünün anjiyojenik yanıt açısından fiyat-etkin, basit, güvenilir ve tekrarlanabilir olması, araştırmacılar için çekici özellik taşımaktadır.
Türk Ortopedi ve Travmatoloji Birliği Derneği dergisi, 2017
Türk Ortopedi ve Travmatoloji Birliği Derneği gerekir. Trombositin yaşlanmasıyla birlikte ortaya ... more Türk Ortopedi ve Travmatoloji Birliği Derneği gerekir. Trombositin yaşlanmasıyla birlikte ortaya çıkan "desializasyon" yani sialik asidin kaybı, hücrenin dolaşımdan uzaklaştırılması için bir sinyal olarak algılanır ve retiküloendoteliyal sistem tarafından dolaşımdan uzaklaştırılır. Hücrelerin en önemli elemanları kuşkusuz proteinlerdir. Her bir hücrenin üstlendiği görevine göre yapısında yer alan protein profili farklıdır ve bu proteinlerin hepsine, o hücrenin proteom'u adı verilir. Trombositlerde nükleus olmadığı için, protein profilinin çok sınırlı ve statik olduğu düşünülebilir. Ancak, megakaryositlerden kendilerine miras kalmış olan proteinler ve intrasitoplazmik mRNA'lar incelendiğinde, trombositlerin çok zengin bir protein profiline sahip olduğu görülür. Sonuç olarak, trombosit protein profilinin kaynağı megakaryositler ve plazmadır. Megakaryositler, kaynağını pluripotent kök hücrelerden alan, hücre bölünmesi olmaksızın çok sayıda DNA replikasyonu (kopyalanarak çoğalma) ile matür (olgun) hale dönüşen sıra dışı hücrelerdir. Asıl önemli rolleri, dolaşıma trombositleri salan çok özgün hücreler olmalarıdır. İnsan bedeni, her gün yaklaşık 100 milyar trombosit yapar. Bunların çok az bir kısmı hemostazda kullanılır; geriye kalan trombositler karaciğer ve dalak sayesinde dolaşımdan uzaklaştırılır. Trombositlerin dolaşımdaki yaşam süreleri yaklaşık 10 gündür. Trombositlerin dolaşımdan uzaklaştırılmalarında (klirensinde), membran proteinlerinin karbonhidrat modifikasyonları oldukça önemlidir. Özellikle membran proteinlerinin glikozillenmiş kısımlarının, sialik asit başlığıyla korunması
In this study, we aimed to evaluate and compare the effects of retrograde intrarenal surgery (RIR... more In this study, we aimed to evaluate and compare the effects of retrograde intrarenal surgery (RIRS) and schok wave lithoripsy (SWL) interventions on renal functions by analyzing glomerular filtration rate (GFR) values. Materials and Methods: A total of 95 patients, who underwent RIRS or SWL in 2017 at Gülhane Training and Research Hospital, were included in this retrospective study. Forty-six of these patients (48.4%) were in RIRS group and 49 (51.6%) were in SWL group. Preoperative, early-postoperative (on the first postoperative day) and late-postoperative (on the first postoperative month) GFR values were calculated using the "abbreviated Modification of Diet in Renal Disease" Method. Changes in GFR values after RIRS and SWL treatments were compared statistically. Results: When the early postoperative and late postoperative GFR levels were compared, a statistically significant difference was observed between the groups (p=0.04 and p<0.001, respectively). For RIRS group, there was a 0.37±13.5 mL/min/1.73 m² increase and for SWL group, there was 5.65±12.5 mL/min/1.73 m² increase in GFR values in the early postoperative period. There was a 2.40±14.1 mL/min/1.73 m² decrease in RIRS group and 7.75±11.8 mL/min/1.73 m² increase in SWL group in GFR values in the late postoperative period. In general linear model, there was a statistically significant difference in changes in GFR over time between RIRS and SWL groups (p=0.002). There was also a statistically significant difference when the changes in GFR over time were compared according to stone locations (p=0.02). Conclusion: RIRS is associated with less improved GFR in comparison with SWL. SWL should be considered as first line treatment for kidney and ureteral stones when considering the changes in GFR values comparing to RIRS especially for stones smaller than 20 mm.
Intestinal ischemia-reperfusion injury (II/R) is a condition with significant morbidity and morta... more Intestinal ischemia-reperfusion injury (II/R) is a condition with significant morbidity and mortality. In this study, we aimed to demonstrate the protective effect of carnosol, an antioxidant, anti-inflammatory, anti-carcinogenic and anti-microbial agent, on pro-inflammatory cytokines, tissue angiopoietins, histopathological damage and bacterial translocation in rats subjected to II/R injury. Thirty male Sprague-Dawley rats were divided into 3 groups: control group, intestinal ischemia/reperfusion group (II/R) and carnosol group (II/R+C). Ischemiareperfusion injury was conducted by laparotomy. All rats received 10 10 colony forming units/milliliters E.coli 12 hours prior to surgery. II/R+C rats received 3 mg/kg/day carnosol per oral starting from 3 days prior to surgery. After 72 hours of post-surgical follow-up, rats were IAAOJ | Health Sciences | 2019 / 5 (2) 106 re-laparotomized for specimen collection. II/R+C group had lower serum TNF-α, IL-1β and IL-6 levels when compared with II/R group (p=0.026, 0.006 and 0.045, respectively). II/R group had significantly higher tissue injury when compared with II/R+C group (p<0.001). II/R+C group rats had significantly reduced liver, spleen and mesenteric lymph node culture growths when compared with II/R group. Angiopoietin I and II levels were similar between groups. In conclusion, carnosol is a powerful anti-inflammatory and anti-microbial agent in II/R injury model. We showed effective reduction of pro-inflammatory cytokine profile, improvement of histopathological damage, and prevention of bacterial translocation.
Increased bone turnover is a hallmark of hyperthyroidism. The underlying factors of how thyroid h... more Increased bone turnover is a hallmark of hyperthyroidism. The underlying factors of how thyroid hormones affect bone cells are still under the spotlight. Previous studies indicated serum osteoprotegerin (OPG), receptor activator of NF-kB ligand (RANKL), and interleukin-6 (IL-6) as mediators of the effect of thyroid hormones on bone metabolism. Ultimately, the present research aimed to examine the association of IL-6 with OPG and RANKL in patients with hyperthyroidism. We carried out this study with 39 newly diagnosed and untreated Graves' patients and 43 healthy controls. In addition to routine tests, we measured serum OPG, RANKL, and IL-6 levels. Results: Mean age and sex distribution were similar in both groups. The hyperthyroid group had significantly higher OPG (p = 0.002) and IL-6 (p < 0.001) levels, but RANKL levels were significantly lower in this group (p < 0.001). We found OPG not to correlate with free T4 and T3, while it had a moderate and negative correlation with thyrotropin (TSH) (r = -0.372, p = 0.001). IL-6 had no correlation with OPG but positively correlated with free T4 (r = 0.445, p < 0.001) and free T3 (r = 0.326, p = 0.035). It also negatively correlated with RANKL (r = -0.247, p = 0.033). Maintaining skeletal development and integrity is partially regulated by a normal balance of thyroid hormones. We concluded that increases in serum OPG and IL-6 levels accompanied hyperthyroidism. However, excessive levels of the hormones might cause drops in serum RANKL levels. Our results suggested that OPG, RANKL, and IL-6 might be involved in the cross-talking among immunity, thyroid function, and bone metabolism in the case of hyperthyroidism.
Anjiyogenez, yeni kapiller oluşumu ile seyreden biyolojik bir süreçtir ve bazı fizyolojik ve pato... more Anjiyogenez, yeni kapiller oluşumu ile seyreden biyolojik bir süreçtir ve bazı fizyolojik ve patolojik durumlarda ortaya çıkmaktadır. Anjiyogenez pozitif ve negatif düzenleyici aktiviteye sahip moleküller arasındaki net dengeyle kontrol edilir. İnsan sağlığı açısından yeni damar oluşumu (anjiyogenez) ve kan damarlarının yayılımının kontrolü (anti-anjiyogenez) oldukça önemlidir. Bu nedenle, anjiyogenez aktif bir araştırma alanıdır. İn vitro modeller, bu süreci tarif etmede faydalı olsalar da, in vivo ne olduğunu yansıtmayabilir. Anjiyogenez araştırmalarında çok yaygın olarak kullanılan, yoğun vaskülarize civciv embriyo koriyoallantoik membran modeli, faydalı bir in vivo sistemdir. Civciv koriyoallantoik membran ölçümünün anjiyojenik yanıt açısından fiyat-etkin, basit, güvenilir ve tekrarlanabilir olması, araştırmacılar için çekici özellik taşımaktadır.
Background. Behçet's disease (BD) is a chronic inflammatory vasculitis affecting multiple organs.... more Background. Behçet's disease (BD) is a chronic inflammatory vasculitis affecting multiple organs. Uveitis is frequently seen in patients with BD, especially in Turkish population. To investigate vascular endothelial growth factor (VEGF) gene polymorphisms along with the levels of VEGF and VEGF receptors in patients with Behçet's uveitis (BU). Fifty-five BD-associated uveitis patients and 30 age-and sex-matched controls were included in this case-control study. The genotypes of the single nucleotide poymorphisms (SNPs): rs2010963 (+405G), rs3025039 (+936T) and rs699947 (-2598A) of the VEGF-A gene were determined using real-time polymerase chain reaction (RT-PCR) and serum levels of VEGF and VEGF receptors were measured using enzyme-linked immunosorbent assay (ELISA). No associations of the VEGF gene polymorphisms were observed in BD uveitis patients, but arthritis was present in 53.3% of patients not possessing CT genotype in C3025039→T polymorphism (p = 0.024). Although there were no statistically significant differences in serum VEGF-A, VEGF-C and soluble vascular endothelial growth factor receptor-3 (sVEGFR-3) levels (p < 0.05), serum vascular endothelial growth factor receptor-1 (VEGFR-1) and sVEGFR-3 levels were significantly higher in the BD group (p < 0.001 and p = 0.001, respectively). In addition, VEGF-C/soluble vascular endothelial growth factor receptor-2 (sVEGFR-2) ratio was significantly higher (p < 0.001), while VEGF-A/VEGFR-1 and VEGF-C/sVEGFR-3 ratios were significantly lower (p < 0.001 and p = 0.033, respectively) in BD patients compared to controls. Also, VEGF-C/sVEGFR-3 (p = 0.024, r = 0.37) and VEGF-C/sVEGFR-2 (p = 0.020, r = 0.38) ratios were positively correlated with disease duration. Conclusions. The significant changes in sVEGFR-3 levels and VEGF-C/sVEGFR-3 ratio has shown that lymphangiogenesis processes might take place in the pathogenesis of BD uveitis, and these parameters can be important indicators of evaluation of BD patients with uveitis together with disease duration.
Introduction: Angiogenesis (neovascularization), which means new vessel construction, is normal a... more Introduction: Angiogenesis (neovascularization), which means new vessel construction, is normal and physiologically, wound healing, embryogenesis, a necessary menstrual cycle it's a mechanism. When taken in appropriate amounts together with or separately with nutrients, mucosal and by regulating systemic immunity, ensuring nutritional and microbial balance in the intestines living nonpatogenic microorganisms that positively affect the health of the host it is called "probiotics". Lactic acid bacteria, the most probiotic microorganisms it constitutes its important group. Where probiotics have an effect on angiogenesis, and it is thought to help heal wounds through the road. With this research indicated that roles of Lactobacillus acidophilus and Lactobacillus rhamnosus on angiogenesis if present to demonstrate in vitro methods and the gene expression responsible for the formation of these effects it is intended to reveal. Material and Method: This study is an experiment...
Intestinal iskemi-reperfuzyon hasari (II/R), onemli morbidite ve mortaliteye sahip bir durumdur. ... more Intestinal iskemi-reperfuzyon hasari (II/R), onemli morbidite ve mortaliteye sahip bir durumdur. Bu calismada, anti-oksidan, anti-enflamatuvar, anti-karsinojenik ve anti-mikrobiyal bir ajan olan karnosolun II/R modeli uygulanan sicanlarda pro-inflamatuar sitokinler, doku anjiopoietinleri, histopatolojik hasar ve bakteriyel translokasyon uzerindeki koruyucu etkisini gostermek amaclanmistir. Otuz erkek Sprague-Dawley sicani 3 gruba ayrildi: kontrol grubu, intestinal iskemi/reperfuzyon grubu (II/R) ve karnosol grubu (II/ R+C). Iskemi-reperfuzyon hasari laparotomi ile saglandi. Tum sicanlara ameliyattan 12 saat once 1010 koloni olusturan birim/mililitre E.coli uygulandi. II/R+C grubuna, ameliyattan 3 gun once baslayarak oral 3 mg/kg/gun karnosol uygulandi. Ameliyat sonrasi takipten 72 saat sonra, sicanlar ornek toplama icin yeniden laparotomize edildi. II/R+C grubunun II/R grubuna gore serum TNF-α, IL-1β ve IL-6 duzeyleri anlamli derecede azalmisti (sirasiyla p=0.026, 0.006 ve 0.045). I...
PurposeLactose intolerance is lactose digestive disorder due to lactase enzyme deficiency. This c... more PurposeLactose intolerance is lactose digestive disorder due to lactase enzyme deficiency. This can affect the quality of life by restricting the intake of certain foods. The aim of this study is to show the lactose intolerance to the restriction in food intake and quality of life.Design/methodology/approachThis study was conducted with adults aged 18–60 years. A survey was used to gather information on the demographic characteristics of the patients and their symptoms related to lactose intolerance. In addition, the Visual Analog Scale was administered to identify common symptoms and the World Health Organization Quality of Life (WHOQOL)-Bref Quality of Life Scale to determine their quality of life.FindingsThe average quality of life subscale scores was 56.25 ± 14.06 for physical, 58.29 ± 11.72 for mental, 63.28 ± 21.35 for social and 62.36 ± 16.37 for environmental. When VAS scores obtained for the common digestive system symptoms in lactose intolerance were compared with Quality ...
To evaluate the stone composition and the distribution of the stone types in the geographical reg... more To evaluate the stone composition and the distribution of the stone types in the geographical regions of Turkey according to where patients were born and where patients live. The patients who were treated for urinary system stone disease in our hospital between January 2011 and March 2019 were included in the study. Stone samples were collected by endoscopic stone surgery methods or shock wave lithotripsy. Stone analyses were made with manual chemical analysis methods. Stone types and distribution were recorded according to geographical regions of Turkey. Results: A total of 706 patients who were treated for urolithiasis were included in the study. Calcium oxalate (CO) stones were at the highest rates for both genders [127 (68.6%) and 359 (68.9%), for females and males, respectively]. There were no statistically significant differences in terms of stone types between genders (p=0.323). There were statistically significant more CO (297), uric acid (17), mixed stones (78) in the Anatolian (central) region than the other regions according to the geographical region where patients were born (p<0.001, for each). Additionally, there were significantly more calcium phosphate stone formers (25) in the Anatolian region according to the geographical region where patients lived (p<0.001). Conclusions: CO is the most common stone type in Turkey independently of where patients were born or where patients live. Higher prevalence rates of stone disease and CO stones were seen in Central Anatolia. Both geographic origin and dietary habits affect the incidence of urinary system stone disease.
Background and Objective: The 5-hydroxytriptamine type 7 receptors (5-HT7Rs) belong to the seroto... more Background and Objective: The 5-hydroxytriptamine type 7 receptors (5-HT7Rs) belong to the serotonin receptor family and modulate many processes in organism and are also found in membrane of endothelial cells (ECs). The LP-211 is a selective agonist of 5-HT7Rs. The purpose of this study was to investigate the effects of activated 5-HT7Rs by LP-211 on regulation of angiogenesis. The effect of LP-211 was tested in vitro by cell proliferation and matrigel assays and in vivo by chick chorioallantoic membrane (CAM) assay. Results: Cell proliferation assay showed that LP-211 had a positive effect on EC proliferation. The LP-211 activated migration of ECs and promoted angiogenesis in matrigel assay in vitro. Stimulation of 5-HT7R by LP-211 caused a significant increase in angiogenic response compared to the control group in CAM assay in vivo. The LP-211, selective agonist of 5-HT7R, was found to have an angiogenic effect in vitro and in vivo. Conclusion: The 5-HT7Rs can be considered to have an important role in EC functions and angiogenesis. Thus, 5-HT7Rs may be a potential target for the therapeutic interventions aimed to regulate the process of angiogenesis in diseases such as varicose veins, peripheral vascular diseases or vasculature of solid tumors. The LP-211 may be a candidate to improve the ineffective circulation in related disorders.
Probiyotikler, gastrointestinal sistem hastalıklarından depresyona kadar çok sayıda hastalık grub... more Probiyotikler, gastrointestinal sistem hastalıklarından depresyona kadar çok sayıda hastalık grubunda çalışılmış ve bu hastalıklar üzerinde probiyotiklerin olumlu etkiler gösterebildiği bildirilmiştir. Probiyotiklerin bildirilen olumlu etkilerini, hastalık gelişimi ve tedavi sürecinde canlı mikrobiyotasını zenginleştirerek gerçekleştirdiği düşünülmektedir. Yara iyileşmesi ise birçok farklı mekanizmanın etkisi altında bulunan bir süreçtir. Bakterilerin bu süreçte faydalı etkileri olduğu gibi farklı bakteri türlerine bağlı olarak olumsuz etkileri de olabilmektedir. Son yapılan çalışmalarda bazı probiyotik bakteri türlerinin topikal uygulaması ya da sistemik etkileri ile yara iyileşmesi üzerine etkili olduğu saptanmıştır. Özellikle gastrik ülserler üzerinde yapılan çalışmalarda probiyotik bakterilerin antibiyotikler ile karşılaştırıldığında, antibiyotik direncinin olduğu durumlarda anjiyogenezanjiyogenez üzerinden etki ederek daha etkili sonuçlar gösterdiği belirtilmiştir. Probiyotikle...
Introduction: Behçet's disease is a chronic inflammatory vasculitis presenting with immunological... more Introduction: Behçet's disease is a chronic inflammatory vasculitis presenting with immunological and endothelial changes. The aim of the present study is to evaluate blood levels of diagnostic markers which can be used in Behçet's patients with vascular involvement. Material and methods: Fifty Behçet's patients (22 with vascular involvement) and 30 healthy controls were included in the study. High-sensitivity C-reactive protein (hsCRP), erythrocyte sedimentation rate (ESR), tumor necrosis factor-α (TNF-α), apolipoprotein A1 (apoA1), apolipoprotein B (apoB), HDL, LDL, triglyceride, total cholesterol, Lp-plA-2, homocysteine and ischemia modified albumin (IMA) levels were analyzed. Statistical analysis was performed with the SPSS program version 11.0. p < 0.05 was accepted as statistically significant. Results: hsCRP, TNF-α, homocysteine, IMA, apoA1, apoB, HDL, Lp-pla2 and ESR levels in patient and control groups were significantly different (p < 0.001, p = 0.001, p < 0.001, p < 0.001, p = 0.005, p < 0.001, p < 0.001, p < 0.001 and p = 0.003 respectively). In Behçet's patients with vascular involvement, homocysteine, TNF-α and Lp-pla2 levels were significantly higher than in Behçet's patients without vascular involvement (p = 0.035, p = 0.010 and p < 0.001 respectively). Conclusions: Increased levels of inflammatory and atherogenic markers in Behçet's patients are an expected outcome due to the inflammatory nature of the disease. Especially, elevated levels of homocysteine, TNF-α and Lp-pla2 make them candidate diagnostic tools to be helpful in clinical evaluation of Behçet's disease patients with vascular involvement.
BACKGROUND/OBJECTIVES: Available data suggest that seasonal changes may influence the nutritional... more BACKGROUND/OBJECTIVES: Available data suggest that seasonal changes may influence the nutritional status and overall health of elderly individuals. Therefore, this study was conducted to investigate the effects of seasonal changes and related factors on energy and nutrient intake of older adults. SUBJECTS/METHODS: Individuals aged 65 years or over were prospectively enrolled in this single-center study (male: 11, female: 20). Data were collected between May 2013 and February 2014 during winter, spring, summer and autumn. Food consumption and biochemical parameters were taken during each season to assess the seasonal nutrition status of the elderly. Upon analysis of biochemical parameters (retinol, vitamin D and vitamin C), an high-performance liquid chromatography device was utilized whereas an Immulite 2000 device was utilized during analysis of serum folic acid and parathyroid hormone. RESULTS: Fruit, fat, egg and bread consumption varied seasonally in males and females (P < 0.05). During winter, daily energy intake was found to be greater than in other seasons in males (557 kcal) and females (330 kcal) (P < 0.05). Additionally, carbohydrates, vegetable protein, n-3 fatty acid and sodium intake increased in winter, while the n-6/n-3 ratio increased in summer among males (P < 0.05). Dietary fiber and sodium intake in winter, vitamin C, iron and zinc intake in spring, and cholesterol, retinol, vitamin D and niacin intake in autumn were found to be higher in females when compared to other seasons (P < 0.05). Serum parathyroid hormone level was higher in winter, and vitamin D level was higher in autumn in both genders (P < 0.05). In males, blood folic acid level was higher in winter, while vitamin C level was higher in females, and there was no seasonal variation in retinol concentration (P < 0.05). CONCLUSION: Food consumption and biochemical parameters showed significant seasonal variations in older adults. It is not clear if nutrition plans in older adults will benefit from consideration of seasonal changes in eating habits.
Diabetes mellitus (DM) is a chronic metabolic disease that requires continuous medical care, wher... more Diabetes mellitus (DM) is a chronic metabolic disease that requires continuous medical care, where the organism cannot adequately benefit from carbohydrates, fats, and proteins due to inherited and/or acquired deficiency in the production of insulin by the β-cells of the pancreas, the ineffectiveness of the produced insulin, or both (1). According to the International Diabetes Federation (IDF) Atlas in 2017, there are about 425 million people with DM, of which one-third are people older than 65 years (atlas.html). According to the World Health Organization (WHO), the number of people with DM increased from 108 million to 422 million between 1980 and 2014 (). Additionally, according to the IDF, over 6.694 million individuals suffer from DM in Turkey and the number of deaths due to DM is approximately 46,300 (2). If left untreated, DM can lead to varying degrees of disability and long-term complications that are not only major health issues but also can generate a number of social and economic problems. Around the world, up to 80% of deaths in patients with DM are closely associated with vascular disease, and in a study evaluating management of DM and DM policies in Turkey, cardiovascular complications were found to account for 32.6% of the total costs of DM, followed by 25% for nephrological complications, 6.4% for eye complications, and 6% for neurological complications (3). Angiostatin, a 38-kDa protein naturally occurring by fragmentation of plasminogen, is a potent endogenous inhibitor of proliferation of endothelial cells and angiogenesis (4). Although its mechanism of action has not yet been completely elucidated, there are many studies in the literature that have focused on the enhancing effect of angiostatin on endothelial cell apoptosis (5-7). The binding of angiostatin to plasma membrane-localized ATP synthetase suppresses the endothelial surface's ATP metabolism and thus downregulates endothelial cell proliferation and migration (8). Background/aim: The clinical effect of angiostatin in diabetes mellitus (DM) patients receiving insulin is a meaningful gap in the literature. In this study, we aimed to show the levels and the clinical significance of angiostatin in DM patients receiving insulin. This is a case-control study. Serum angiostatin levels were determined by ELISA. A total of 83 people consisting of healthy subjects (n = 36) and patients with a diagnosis of DM receiving insulin therapy (n = 47) were included in this study. The mean angiostatin levels of the DM group were significantly higher than those of the control group (86.0 ± 68.1 ng/mL and 58.0 ± 22.4 ng/mL, respectively; P = 0.011). Significantly lower angiostatin levels were determined in the DM patients receiving metformin with respect to those not receiving metformin (97.2 ± 74.4 ng/mL and 49.3 ± 7.0 ng/mL, respectively; P = 0.021). Significantly higher levels of angiostatin were observed among the DM patients using a beta-blocker (BB) than the DM patients not using a BB (115.5 ± 78.71 ng/mL and 73.44 ± 60.08 ng/mL, respectively; p = 0.029). This is the first study evaluating and demonstrating the serum angiostatin levels in DM patients receiving insulin. Further studies are required to understand the effect of angiostatin in diabetics and the effect of medications on angiogenesis in these patients.
Objectives. High morbidity and mortality rates due to chronic heart diseases in patients on hemod... more Objectives. High morbidity and mortality rates due to chronic heart diseases in patients on hemodialysis is a well-known entity. The main lipid disorder leading to atherosclerosis in these patients is LDL-cholesterol (LDL-C) elevation. Recently, it has been suggested that a decrease in ...
Angiogenesis is the process of generating new blood vessels from preexisting vessels and is consi... more Angiogenesis is the process of generating new blood vessels from preexisting vessels and is considered essential in many pathological conditions. The purpose of the present study is to evaluate the effect of aspartame on angiogenesis in vivo chick chorioallantoic membrane (CAM) and wound-healing models as well as in vitro 2,3-bis-2 H-tetrazolium-5-carboxanilide (XTT) and tube formation assays. In CAM assay, aspartame increased angiogenesis in a concentration-dependent manner. Compared with the control group, aspartame has significantly increased vessel proliferation ( p < 0.001). In addition, in vivo rat model of skin wound-healing study showed that aspartame group had better healing than control group, and this was statistically significant at p < 0.05. There was a slight proliferative effect of aspartame on human umbilical vein endothelial cells on XTT assay in vitro, but it was not statistically significant; and there was no antiangiogenic effect of aspartame on tube format...
Anjiyogenez, yeni kapiller oluşumu ile seyreden biyolojik bir süreçtir ve bazı fizyolojik ve pato... more Anjiyogenez, yeni kapiller oluşumu ile seyreden biyolojik bir süreçtir ve bazı fizyolojik ve patolojik durumlarda ortaya çıkmaktadır. Anjiyogenez pozitif ve negatif düzenleyici aktiviteye sahip moleküller arasındaki net dengeyle kontrol edilir. İnsan sağlığı açısından yeni damar oluşumu (anjiyogenez) ve kan damarlarının yayılımının kontrolü (anti-anjiyogenez) oldukça önemlidir. Bu nedenle, anjiyogenez aktif bir araştırma alanıdır. İn vitro modeller, bu süreci tarif etmede faydalı olsalar da, in vivo ne olduğunu yansıtmayabilir. Anjiyogenez araştırmalarında çok yaygın olarak kullanılan, yoğun vaskülarize civciv embriyo koriyoallantoik membran modeli, faydalı bir in vivo sistemdir. Civciv koriyoallantoik membran ölçümünün anjiyojenik yanıt açısından fiyat-etkin, basit, güvenilir ve tekrarlanabilir olması, araştırmacılar için çekici özellik taşımaktadır.
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