Papers by Svetlana Mankovskaya
Restorative neurology and neuroscience, Feb 15, 2024
Cancers
Childhood papillary thyroid carcinoma (PTC) diagnosed after the Chernobyl accident in Belarus dis... more Childhood papillary thyroid carcinoma (PTC) diagnosed after the Chernobyl accident in Belarus displayed a high frequency of gene rearrangements and low frequency of point mutations. Since 2001, only sporadic thyroid cancer occurs in children aged up to 14 years but its molecular characteristics have not been reported. Here, we determine the major oncogenic events in PTC from non-exposed Belarusian children and assess their clinicopathological correlations. Among the 34 tumors, 23 (67.6%) harbored one of the mutually exclusive oncogenes: 5 (14.7%) BRAFV600E, 4 (11.8%) RET/PTC1, 6 (17.6%) RET/PTC3, 2 (5.9%) rare fusion genes, and 6 (17.6%) ETV6ex4/NTRK3. No mutations in codons 12, 13, and 61 of K-, N- and H-RAS, BRAFK601E, or ETV6ex5/NTRK3 or AKAP9/BRAF were detected. Fusion genes were significantly more frequent than BRAFV600E (p = 0.002). Clinicopathologically, RET/PTC3 was associated with solid growth pattern and higher tumor aggressiveness, BRAFV600E and RET/PTC1 with classic papi...
This chapter presents the results of studies on pediatric papillary thyroid carcinoma (PTC) in Be... more This chapter presents the results of studies on pediatric papillary thyroid carcinoma (PTC) in Belarus after the Chernobyl accident. Our investigations are based on a scrupulous histopathological review of all cases by a group of experienced pathologists with special skills in thyroid neoplasia, who confirmed the diagnosis, and determined the extent of disease and tumor characteristics using the up-to-date criteria. Lymph and/or blood vessel invasion, desmoplastic stromal reaction (fibrosis), the presence and distribution of psammoma bodies, PTC variants, tumor growth pattern (architectonics), etc., were used as variables tested for association with regional and distant metastases at presentation, and disease recurrence. In addition, age at diagnosis, age at exposure, and the duration of latency were shown to independently affect clinical and morphological features of PTC. Treatment strategy and further thyroid cancer management in children and adolescents should take into account t...
Updates in the Understanding and Management of Thyroid Cancer, 2012
Updates in the Understanding and Management of Thyroid Cancer 164 adolescents with the history of... more Updates in the Understanding and Management of Thyroid Cancer 164 adolescents with the history of exposure to radioactive isotopes of iodine in the framework of the Chernobyl Reactor Accident in 1986. Thyroid cancer is uncommon in childhood and adolescence. The incidence of all thyroid cancer types in children and adolescents varies from 0.04-0.17 per 100 000 in Ukraine, Germany, and Russia up to 0.54 in the USA (
Cancer Letters, 2005
The study was set out to determine whether characteristic changes in the gene expression profile ... more The study was set out to determine whether characteristic changes in the gene expression profile in papillary thyroid carcinoma (PTC) discovered by microarray assays can be used for conventional molecular diagnosis. Expression levels of five reported to be overexpressed and three underexpressed genes were examined in PTC and normal human tissues by realtime PCR and semi-quantitative duplex PCR. Stepwise logistic regression analysis, duplex PCR data evaluation with recursive partition machine algorithm and hierarchical cluster analysis identified SFTPB (upregulated) and TFF3 (downregulated) gene combination as most favorable for differential molecular diagnosis of PTC. Sensitivity, specificity and accuracy obtained in a series of histologically characterized thyroid tumor and normal tissue samples were 88.9, 96.7 and 94.9%, respectively. Applicability of the method to fine needle aspiration biopsy (FNAB) samples was demonstrated using a collection of needle washouts. In spite individual thyroid tumor and normal tissues as well as FNAB samples displayed a substantial degree of variability in the expression levels of analyzed genes, simultaneous molecular analysis of a panel of optimal markers allows making a high probability predictive estimate and may be considered as an informative method of preoperative PTC diagnosis.
Doklady Nacionalʹnoj akademii nauk Belarusi, Apr 29, 2024
Аннотация. Впервые в эксперименте in vivo установлен положительный сочетанный (ангиогенный и анти... more Аннотация. Впервые в эксперименте in vivo установлен положительный сочетанный (ангиогенный и антиноцицептивный) эффект применения отечественной генноинженерной конструкции рcDNA_VEGF165 в условиях моделированной ишемии мышц конечности. Плазмида с геном, кодирующим белок VEGF165 (рcDNA_VEGF165), может являться основой для создания первых в Республике Беларусь генотерапевтических лекарственных средств. Ключевые слова: хроническая ишемия нижних конечностей, генная терапия, плазмида, фактор роста эндотелия сосудов, VEGF Для цитирования. Ангиогенные и антиноцицептивные эффекты генотерапевтической конструкции рcDNA_ VEGF165 в условиях хронической ишемии конечности в эксперименте in vivo / В. Г. Богдан [и др.] // Докл. Нац. акад.
The Journal of clinical endocrinology and metabolism, 2014
The aim of this population-based study was to evaluate the clinical and pathological characterist... more The aim of this population-based study was to evaluate the clinical and pathological characteristics and outcome of papillary thyroid carcinoma (PTC) that have arisen in the Belarusian childhood population exposed to the radioactive fallout from the Chernobyl accident within a long-term period. The long-term treatment results were investigated in 1078 children and adolescents (<19 years old) with PTC who were surgically treated during the years 1990 through 2005. Patients had high rates of metastatic PTC at presentation, with 73.8% of cases having lymph node involvement and 11.1% having distant spread. The most influential factor for lymph node metastases at initial treatment was lymphatic vessel invasion (P < .0001) and for distant metastases, lateral lymph node involvement (P < .0001). The overall survival was 96.9% ± 0.9% with a median follow-up of 16.21 years, and 20-year event-free survival and relapse-free survival were 87.8% ± 1.6% and 92.3% ± 0.9%, respectively. Pat...
Proceedings of the National Academy of Sciences of Belarus, Biological Series, 2022
The study of wound-healing effect of the gel substances with 2 and 4 % (w/w) concentrations of th... more The study of wound-healing effect of the gel substances with 2 and 4 % (w/w) concentrations of the dry extracts of the everlasting flowers and of the littlewale leaves in the ratio 1:1 were carried out.It was found that the skin application of the studied gel substances promoted the formation of the structural and functional regenerate in the area of the wound defect. The studied gel contributed to the significant reduction of the healing duration of the full-thickness linear wounds in rats compared to the control group of animals (without treatment) and to the group of animals that received treatment with the reference listed drug. The use of the studied gels, regardless of the content of the plant extracts in them (2 or 4 % (w/w)), was not accompanied by toxic effects.
The parameters of specific triidothyronine binding with lymphocytes of patients operated for high... more The parameters of specific triidothyronine binding with lymphocytes of patients operated for highly differentiated thyroid cancer and received comprehensive treatment, on the average, for 15 years are studied. These patients have revealed a significant increase of interaction affinity and a decrease in the number of binding places in comparison with these characteristics of the persons of the control group. A relationship of the studied indices between the factors of treatment and its duration is shown. Patients with overweight have revealed a significant increase in interaction affinity and a decrease in the number of binding places as compared to patients with normal weight, which explains a decreased need in triiodothyronine in this group of patients.
Thyroid, 2012
Thyroid Clinical and pathologic features of 'sporadic' papillary thyroid carcinoma registered in ... more Thyroid Clinical and pathologic features of 'sporadic' papillary thyroid carcinoma registered in 2005-2008 years in children and adolescents of Belarus (
Voprosy onkologii, 2014
There is presented clinical and morphological characteristics of post-Chernobyl papillary thyroid... more There is presented clinical and morphological characteristics of post-Chernobyl papillary thyroid cancer in 936 children and adolescents. In general, carcinoma of these patients featured by locally advanced growth - 57.4% (387 of 674 patients with this sign could be assessed), metastases in regional lymph nodes - 73,7% (N1b in 40.7%) and internal organs - 11.1%. The mean duration of follow-up was 12,4 +/- 3,5 years (range 4.3 to 19.6 years) including children 14,6 +/- 2,7 years (range 8.8 to 19.6 years) and adolescents - 10,1 +/- 3,1 years (range 4.3 to 18.8 years). Overall survival for the 20-year period was 96,6% +/- 1,2%. The causes of death were suicide (7), injuries and accidents (5), secondary malignancies (1), somatic diseases (2). Only in two patients the death was related to the main disease - lung metastases. Free-recurrence survival for the cohort of post-Chernobyl carcinomas was 92,7% +/- 1,0%.
International Congress Series, 2007
The retrospective analysis was performed to compare the clinical course of radiogenic and sporadi... more The retrospective analysis was performed to compare the clinical course of radiogenic and sporadic childhood thyroid cancer. The entire series included 752 patients aged less than 15 years at diagnosis. Of them, 686 (91.2%) individuals had radiation history including 681 (90.6%) exposed at the time of the Chernobyl disaster and 5 (0.7%) children were previously treated for malignant lymphoma with an external beam therapy. The second group consisted of 66 (8.8%) unexposed patients. All the children underwent surgery and received suppressive thyroxine therapy with the mean dose of 2.0-2.5 μg/kg of body weight. Radioiodine therapy was performed in 464 (61.7%) cases. The comparison of patients with radiogenic and sporadic thyroid cancer did not reveal statistically significant differences in the distribution of tumor size, histological type or TNM stage. During follow-up time relapses were registered in 204 (27.1%) patients. The main sites of recurrence were the lung or lymph nodes whereas other lesions (thyroid remnants, the bone, soft tissues or CNS involvement) were uncommon. The disease-free survival ranged widely, 0-213 months after the date of primary surgery without significant difference between the radiogenic and sporadic cases. The observed 10-year survival was 99.5% for the entire series, including 99.5%
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клинико-МорФологиЧЕскиЕ осоБЕнносТи пАпиллЯрного рАкА ЩиТоВиДноЙ ЖЕлЕЗы У ДЕТЕЙ и поДросТкоВ В рЕ... more клинико-МорФологиЧЕскиЕ осоБЕнносТи пАпиллЯрного рАкА ЩиТоВиДноЙ ЖЕлЕЗы У ДЕТЕЙ и поДросТкоВ В рЕспУБликЕ БЕлАрУсЬ: АнАлиЗ 936 посТ-ЧЕрноБылЬскиХ кАрЦиноМ 1 Белорусская медицинская академия последипломного образования 2 Институт физиологии НАН Беларуси 3 Объединенный институт проблем информатики НАН Беларуси, Минск представлена клинико-морфологическая характеристика пост-чернобыльского папиллярного рака щитовидной железы у 936 детей и подростков. В целом, карцинома у данных пациентов отличалась местно-распространенным ростом -в 57,4% (387 из 674 пациентов, у которых этот признак можно было оценить), метастазами в регионарных лимфатических узлах -в 73,7% (N1b у 40,7%) и внутренних органах -11,1%. средняя продолжительность периода наблюдения составила 12,4±3,5 лет (от 4,3 до 19,6 лет), в том числе у детей 14,6±2,7 лет (от 8,8 до 19,6 лет), а у подростков -10,1±3,1 лет (от 4,3 до 18,8 лет). общая выживаемость за 20-летней период равнялась 96,6%±1,2%. причиной смерти стали суициды (7), травмы и несчастные случаи (5), вторые злокачественные опухоли (1), соматические заболевания (2). Только у двух больных летальный исход был связан с основным заболеванием -метастатическим поражением легких. Безрецидивная выживаемость для когорты пост-чернобыльских карцином составила 92,7%±1,0%. ключевые слова: папиллярный рак, щитовидная железа, дети и подростки, Чернобыльская авария
Acta medica Lituanica, 2010
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Voprosy onkologii, 2014
There is presented clinical and morphological characteristics of post-Chernobyl papillary thyroid... more There is presented clinical and morphological characteristics of post-Chernobyl papillary thyroid cancer in 936 children and adolescents. In general, carcinoma of these patients featured by locally advanced growth - 57.4% (387 of 674 patients with this sign could be assessed), metastases in regional lymph nodes - 73,7% (N1b in 40.7%) and internal organs - 11.1%. The mean duration of follow-up was 12,4 +/- 3,5 years (range 4.3 to 19.6 years) including children 14,6 +/- 2,7 years (range 8.8 to 19.6 years) and adolescents - 10,1 +/- 3,1 years (range 4.3 to 18.8 years). Overall survival for the 20-year period was 96,6% +/- 1,2%. The causes of death were suicide (7), injuries and accidents (5), secondary malignancies (1), somatic diseases (2). Only in two patients the death was related to the main disease - lung metastases. Free-recurrence survival for the cohort of post-Chernobyl carcinomas was 92,7% +/- 1,0%.
The Journal of clinical endocrinology and metabolism, 2014
The aim of this population-based study was to evaluate the clinical and pathological characterist... more The aim of this population-based study was to evaluate the clinical and pathological characteristics and outcome of papillary thyroid carcinoma (PTC) that have arisen in the Belarusian childhood population exposed to the radioactive fallout from the Chernobyl accident within a long-term period. The long-term treatment results were investigated in 1078 children and adolescents (<19 years old) with PTC who were surgically treated during the years 1990 through 2005. Patients had high rates of metastatic PTC at presentation, with 73.8% of cases having lymph node involvement and 11.1% having distant spread. The most influential factor for lymph node metastases at initial treatment was lymphatic vessel invasion (P < .0001) and for distant metastases, lateral lymph node involvement (P < .0001). The overall survival was 96.9% ± 0.9% with a median follow-up of 16.21 years, and 20-year event-free survival and relapse-free survival were 87.8% ± 1.6% and 92.3% ± 0.9%, respectively. Pat...
Updates in the Understanding and Management of Thyroid Cancer, 2012
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Papers by Svetlana Mankovskaya