International Journal of Fisheries and Aquatic Studies, 2017
A problem often faced by grouper fish exporters is the high mortality rate (97%) of the fish due ... more A problem often faced by grouper fish exporters is the high mortality rate (97%) of the fish due to stress during transport. The present study was conducted to analyzethe effect of clove oil on blood glucose levels and the number of erythrocyte during close transport system. This experimental design used complete randomized design (CRD). The data obtained in the study were analyzed using statistical test ANOVA (Analysis of Variance) to determine whether there were differences among the treatments, followed by Duncan's multiple range test. Results of the study showed blood glucose value of P< 0.05, indicating significant difference in the treatment of clove oil. Duncan's multiple range test showed that there were significant differences among the treatments P2 (MS-222 70 ppm) with P1 (negative control), P3 (clove oil 5 ppm), P4 (clove oil 10 pp) and P5 (clove oil 15 ppm). Total erythrocyte estimation shows the value of p>0.05, indicating no significant difference in clove oil treatment. In this study,Duncan's multiple range test showed no significant differences among treatments.
International Journal of Fisheries and Aquatic Studies, 2018
White spot Baculovirus (WSBV) infection of shrimp is characterized by high mortality of tiger shr... more White spot Baculovirus (WSBV) infection of shrimp is characterized by high mortality of tiger shrimp Penaeus monodon. The gross signs of moribund shrimp are present of white spot, initially circular, inside cuticle, sometimes accompanied by overall red body coloration. The 5 major protein were isolated from WSBV are 15 kDa (VP15), 19 kDa (VP19), 24 kDa (VP24), 26 kDa (VP26) and 28 kDa (VP28). The isolated protein were then purified and characterized to be VP19, VP24, and VP28. Purified protein injected to shrimp to detect whether they have a protective immunity against WSBV infection. The result showed protection of infected shrimp against WSBV after administration of VP19, VP24 and VP28 are 20%, 0% and 60%, respectively. VP28 injected shrimp have a high production of phenoloxidase 24.1822 U/min/mg and after WSBV challenged 44.6067 U/min/mg. Based on the result suggest that VP28 of WSBV is potentially to increase immune response in penaeid shrimp.
International Journal of Fisheries and Aquatic Studies, 2019
The aims of the research are to determine the hematological response of Java Barb which is expose... more The aims of the research are to determine the hematological response of Java Barb which is exposed to heavy metal lead (Pb) at different doses. Water pollution occurs due to industrial activities that contain toxic materials which are increasing with the development of industrialization. One of the heavy metals that cause pollution in Sidoarjo, East Java Indonesia waters is lead (Pb). Heavy metal in waters at can affect the life of aquatic biota, especially fish. Lead (Pb) enter the body of an organism through the food chain, gills or diffusion through the surface of the skin. Lead enters the fish cause toxic effect that increase in oxidative stress, organ damage and changes in the biochemical profile of the blood which indicates metabolism in the body is disturbed. Metabolic disorders will affect the growth of cultivated fish Java Barb (Barbonymus gonionotus), is one type of freshwater fish that is widely cultivated in Indonesia but is very sensitive to changes of water quality. The research was an experimental using a completely randomized design consisting of 5 treatments and 4 replications. The treatment are differences in lead doses, namely A (0 ppm), B (0.66 ppm), C (1.32 ppm), D (1.98 ppm) and E (2.65 ppm). The parameters of observation were total erythrocytes, hemoglobin, total leukocytes and leukocytes differential, blood glucose and cortisol. Data obtained were analyzed using Anova to determine the effect of treatment. Differences between treatments were analyzed using Duncan's multiple distance test. The results showed that distribution of different lead doses increase the number of leukocytes and reduce the total erythrocytes, hemoglobin hematocrit, leukocytes differential, blood glucose and cortisol.
International Journal of Fisheries and Aquatic Studies, 2017
A problem often faced by grouper fish exporters is the high mortality rate (97%) of the fish due ... more A problem often faced by grouper fish exporters is the high mortality rate (97%) of the fish due to stress during transport. The present study was conducted to analyzethe effect of clove oil on blood glucose levels and the number of erythrocyte during close transport system. This experimental design used complete randomized design (CRD). The data obtained in the study were analyzed using statistical test ANOVA (Analysis of Variance) to determine whether there were differences among the treatments, followed by Duncan's multiple range test. Results of the study showed blood glucose value of P< 0.05, indicating significant difference in the treatment of clove oil. Duncan's multiple range test showed that there were significant differences among the treatments P2 (MS-222 70 ppm) with P1 (negative control), P3 (clove oil 5 ppm), P4 (clove oil 10 pp) and P5 (clove oil 15 ppm). Total erythrocyte estimation shows the value of p>0.05, indicating no significant difference in clove oil treatment. In this study,Duncan's multiple range test showed no significant differences among treatments.
International Journal of Fisheries and Aquatic Studies, 2018
White spot Baculovirus (WSBV) infection of shrimp is characterized by high mortality of tiger shr... more White spot Baculovirus (WSBV) infection of shrimp is characterized by high mortality of tiger shrimp Penaeus monodon. The gross signs of moribund shrimp are present of white spot, initially circular, inside cuticle, sometimes accompanied by overall red body coloration. The 5 major protein were isolated from WSBV are 15 kDa (VP15), 19 kDa (VP19), 24 kDa (VP24), 26 kDa (VP26) and 28 kDa (VP28). The isolated protein were then purified and characterized to be VP19, VP24, and VP28. Purified protein injected to shrimp to detect whether they have a protective immunity against WSBV infection. The result showed protection of infected shrimp against WSBV after administration of VP19, VP24 and VP28 are 20%, 0% and 60%, respectively. VP28 injected shrimp have a high production of phenoloxidase 24.1822 U/min/mg and after WSBV challenged 44.6067 U/min/mg. Based on the result suggest that VP28 of WSBV is potentially to increase immune response in penaeid shrimp.
International Journal of Fisheries and Aquatic Studies, 2019
The aims of the research are to determine the hematological response of Java Barb which is expose... more The aims of the research are to determine the hematological response of Java Barb which is exposed to heavy metal lead (Pb) at different doses. Water pollution occurs due to industrial activities that contain toxic materials which are increasing with the development of industrialization. One of the heavy metals that cause pollution in Sidoarjo, East Java Indonesia waters is lead (Pb). Heavy metal in waters at can affect the life of aquatic biota, especially fish. Lead (Pb) enter the body of an organism through the food chain, gills or diffusion through the surface of the skin. Lead enters the fish cause toxic effect that increase in oxidative stress, organ damage and changes in the biochemical profile of the blood which indicates metabolism in the body is disturbed. Metabolic disorders will affect the growth of cultivated fish Java Barb (Barbonymus gonionotus), is one type of freshwater fish that is widely cultivated in Indonesia but is very sensitive to changes of water quality. The research was an experimental using a completely randomized design consisting of 5 treatments and 4 replications. The treatment are differences in lead doses, namely A (0 ppm), B (0.66 ppm), C (1.32 ppm), D (1.98 ppm) and E (2.65 ppm). The parameters of observation were total erythrocytes, hemoglobin, total leukocytes and leukocytes differential, blood glucose and cortisol. Data obtained were analyzed using Anova to determine the effect of treatment. Differences between treatments were analyzed using Duncan's multiple distance test. The results showed that distribution of different lead doses increase the number of leukocytes and reduce the total erythrocytes, hemoglobin hematocrit, leukocytes differential, blood glucose and cortisol.
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