Growing consumer awareness of the potential negative health effects of synthetic antibiotics has ... more Growing consumer awareness of the potential negative health effects of synthetic antibiotics has prompted the search for more natural preservatives that can improve the safety and quality of food. In this study we report the enzymatic synthesis of N-α-[Carbobenzyloxy]-Ile-Gln (Z-IQ) which is the precursor of Ile-Gln (IQ), a new antibacterial dipeptide, using an aqueous–organic biphasic system formed by 50% (v/v) ethyl acetate in 0.1 M Tris – HCl buffer pH 8. A partially purified proteolytic extract from the fruits of Solanum granuloso leprosum, named granulosain, proved to be a robust biocatalyst for the synthesis of Z-IQ, eliciting 71 ± 0.10% maximal peptide yield in the above described conditions. After cleaving and purifying IQ dipeptide, antimicrobial activity was assayed against Staphylococcus aureus ATCC 25923, Staphylococcus hominis A17771, and Staphylococcus aureus C00195, and MIC values between 118 ± 0.01 μg/mL and 133.7 ± 0.05 μg/mL were obtained. In addition, IQ showed MI...
Colegio de Farmacéuticos de la Provincia de Buenos Aires, Jul 1, 2019
The rapid increase in drug-resistant infections has presented a serious challenge to antimicrobia... more The rapid increase in drug-resistant infections has presented a serious challenge to antimicrobial therapies, encouraging the development of novel control agents. Natural antimicrobial compounds for food have gained attention due to the rise of tolerant microorganisms to food preservation methods. The aim of this work is to evaluate the antibacterial activity of granulosain, the pre-purified proteolytic extract obtained from the fruits of Solanum granuloso-leprosum Dunal, as potential antibacterial compound for the pharmaceutical and food industries. Antibacterial activity of granulosain was tested against representative Gram-positive and Gram-negative strains, in batch culture of Müller-Hinton broth, at 37 ºC and 180 rpm. According to Kruskal-Wallis test, granulosain shows no inhibition of Staphylococcus aureus ATCC 25923, within the studied range. In contrast, granulosain was able to elicit significantly decrease (p ≤ 0.05) on the growth of Escherichia coli ATCC 25922 at protein concentrations equal to or greater than 50 μg/mL.El rápido aumento de las infecciones resistentes a los medicamentos ha presentado un serio desafío para las terapias antimicrobianas, alentando el desarrollo de nuevos agentes de control. Los compuestos antimicrobianos naturales para alimentos han ganado atención debido al aumento de microorganismos tolerantes a los métodos de conservación de alimentos. El objetivo de este trabajo es evaluar la actividad antibacteriana de granulosaína, el extracto proteolítico pre-purificado obtenido de los frutos de Solanum granuloso-leprosum Dunal, como potencial compuesto antibacteriano para las industrias farmacéutica y alimentaria. La actividad antibacteriana de granulosain se probó contra cepas representativas de grampositivos y gramnegativos, en cultivo discontinuo de caldo Müller-Hinton, a 37 ºC y 180 rpm. Según la prueba de Kruskal-Wallis, granulosaína no muestra inhibición de Staphylococcus aureus ATCC 25923, dentro del rango estudiado. Por el contrario, granulosaína fue capaz de provocar una disminución significativa (p ≤ 0.05) en el crecimiento de Escherichia coli ATCC 25922 a concentraciones de proteína iguales o superiores a 50 μg/mL.Fil: Adaro, Mauricio Omar. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Bromatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; ArgentinaFil: Vallés, Diego. Universidad de la República. Facultad de Ciencias; UruguayFil: Cantera, Ana M.. Universidad de la República. Facultad de Ciencias; UruguayFil: Talia, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Barberis, Sonia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Bromatología; Argentin
El incremento de la sensibilidad humana a los alergenos, en particular al polen, tiene relación c... more El incremento de la sensibilidad humana a los alergenos, en particular al polen, tiene relación con factores medioambientales y con el estilo de vida. La cantidad total de polen observado en el aire está creciendo debido a los cambios climáticos. En Europa la floración de muchos árboles y hierbas empieza antes y dura más que hace 10 años, debido a la interacción entre el aumento de la temperatura y la concentración de CO2. Además, los contaminantes químicos del aire pueden modificar las características de los alergenos, entre los que se incluye el polen, aumentando la sensibilidad y las respuestas alérgicas de la población. El tipo de alergia respiratoria más frecuente es causado por la presencia de polen en el aire y se denomina polinosis. Las plantas polinizan durante el día, pero el polen queda en suspensión muchas horas. La concentración de polen en el aire decrece con la distancia, por lo que un solo árbol en un jardín puede tener más repercusiones en la salud que un bosque a 1...
Helicobacter pylori is a gram-negative, helix-shaped, and microaerophilic bacteria that colonizes... more Helicobacter pylori is a gram-negative, helix-shaped, and microaerophilic bacteria that colonizes the human gastric mucosa, causing chronic infections, gastritis, peptic ulcer, lymphomas associated with lymphoid mucosa tissue, and gastric cancer. H. pylori is considered a Type 1 human carcinogen by WHO. The prevalence of the infection is estimated in more than half of the world population. Treatment of H. pylori infection includes antibiotics and proton pump inhibitors, but the increasing antibiotic resistance promotes the research of novel, more effective, and natural antibacterial compounds. The aim of this work was to study the effect of the partially purified proteolytic extract (RAP) of the fruits from Solanum granuloso-leprosum (Dunal), a South American native plant, and a purified fraction named granulosain I, against H. pylori, to obtain natural food additives for the production of anti-H. pylori functional foods. Furthermore, granulosain I and RAP could be used as natural a...
How to cite Complete issue More information about this article Journal's homepage in redalyc.org ... more How to cite Complete issue More information about this article Journal's homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative
SUMMARY. The effect of different organic solvents on the stability of araujiain (the crude enzyme... more SUMMARY. The effect of different organic solvents on the stability of araujiain (the crude enzyme preparation obtained from the latex of fruits of Araujia hortorum Fourn., Asclepiadaceae) in mixtures of buffer and different water-miscible or immiscible organic solvents, and in continuous systems (organic solvents with low water activity) after 4 h at 40 oC was studied in this paper in order to select the most adequate media for peptide enzymatic synthesis. Araujiain showed the highest stability in 50% hexane, 50% propanone, and N,N-dimethylformamide (a w : 0.5 in the enzyme), and those values were remarkably greater than in buffer. In addition, the presence of those organic solvents reduced the autolysis degree. In general, araujiain was not inactivated in aqueous-miscible organic systems and the enzyme showed higher activities in those media than in buffer. In biphasic systems, the partition of the organic solvents into the aqueous phase activated the enzyme in several cases. These...
Antiacanthain and granulosain are the partially purified proteolytic extracts from the South Amer... more Antiacanthain and granulosain are the partially purified proteolytic extracts from the South American native fruits of Bromelia antiacantha (Bertol. ) and Solanum granuloso leprosum, respectively. The aim of this work was to compare the ability of both soluble and immobilized antiacanthain and granulosain f or the synthesis of Z-Tyr-Val-OH, a novel antibacterial dipeptide, in different reaction systems formed by almost anhydrous organic solvents (Xw: 1 × 10−5) and several percentages of immiscible organic solvents in 100 mM Tris(hydroxymethyl)aminomethane hydrochloride buffer pH 8.0. Soluble antiacanthain in half of the 24 different organic biphasic media showed higher catalytic potential than in 100 mM Tris(hydroxymethyl)aminomethane hydrolchloride buffer pH 8.0. Soluble granulosain showed lower catalytic potential in all liquid-liquid biphasic media than in the same buffer. However, 50% (v/v) ethyl ethanoate in 100 mM Tris(hydroxymethyl)aminomethane hydrolchloride buffer pH 8.0 al...
Proteins have functional properties that govern their behavior in foods during processing, storag... more Proteins have functional properties that govern their behavior in foods during processing, storage, and consumption. Proteins can have high nutritional quality and not have functional properties suitable for incorporation into determined food systems. Furthermore, a desirable functional attribute for an additive may be undesirable for everyone else. This chapter describes the design of a new strategy to predict, control, and optimize the functional parameters of food proteins hydrolyzed or not, using chemometrics tools. The starting material consists of proteins whose functional properties are desired to modify. Functional properties (e.g., emulsifying and foaming properties) can be simultaneously evaluated by an experimental statistical design, response surface graphics, and multiple linear regressions. This strategy expands the applications of food proteins and allows the following facilities: assess interactions between variables of multivariate systems, evaluate the dependence b...
Biotechnological Applications of Plant Proteolytic Enzymes
Proteolytic enzymes (proteases) comprise a group of hydrolases (EC 3.4, NC-IUBMB) which share the... more Proteolytic enzymes (proteases) comprise a group of hydrolases (EC 3.4, NC-IUBMB) which share the common feature of acting on peptide bonds. Proteases are among the best-studied enzymes in terms of structure–function relationship (Krowarsch et al. 2005). Proteases, by catalyzing the cleavage of other proteins and even themselves, have an enormous physiological significance, their coding genes representing as much as 2% of the total human genome (Schilling and Overall 2008).
Third International Conference on Advances in Bio-Informatics and Environmental Engineering - ICABEE 2015, Dec 11, 2015
The aim of this work is to study key functional parameters of the goat cheese protein hydrolysate... more The aim of this work is to study key functional parameters of the goat cheese protein hydrolysates. A Plackett-Burman Statistical Design, Response Surface Methodologies and Artificial Neural Network are applied to describe the effects of different factors (pH, Temperature, Time of hydrolysis, Amount of added buffer and Enzyme : Substrate ratio) on the following functional parameters of goat cheese proteins, which are hydrolysed by papain: Free Amine Nitrogen (NA), Total Soluble Nitrogen (NT), Solubility (PSI), Water Holding Capacity (WHC), Emulsifying Activity Index (EAI), Emulsifying Stability Index (ESI), Viscosity (), Held Water (HW), Surface Hydrophobicity (So), Foaming Capacity (FC) and Foam Stability (FS). According to our results, the release of soluble proteins from goat cheese to the supernatant (NT) and the hydrolysis degree of proteins into the supernatant (NA) increased until 443% and 273%, respectively. PSI, WHC, EAI, ESI and HW increased until 443%, 159%, 0.88%, 324% and 64% respectively. decreased until 33% and So (bitter peptides indicator) was reduced until 98.8 %, regard to the original protein isolates. FC and FS were extremely low or null. Predicted values were experimentally confirmed and compared with those of original protein isolates.
Third International Conference on Advances in Bio-Informatics and Environmental Engineering - ICABEE 2015
The aim of this work is to evaluate the mineral and heavy metal contents of Acacia caven (Mol.) M... more The aim of this work is to evaluate the mineral and heavy metal contents of Acacia caven (Mol.) Molina pollen, a South American plant which grows in the Northwest of Argentina, for studying its behavior as bio-indicator of environmental pollution and their consequences as dietary supplement. Mineral and heavy metal contents were determined by flame atomic absorption / emission spectrometry (FAAS and FAES, respectively), inductively coupled plasma optical emission spectrometry (ICP-OES) and hydride generation atomic absorption spectrometry (HG-AAS). The obtained results demonstrated that some mineral contents (K, Ca, P, Fe, Mg, Na, Zn and Se) of Acacia caven pollen met the requirements of both, the Food and Nutritional Board (FNB) and the Argentinean Alimentarius Codex (AAC) to be used as a dietary supplement. Nevertheless, some heavy metals analyzed such as Al, Cu, Ni, V, Cr and Cd, exceeded the upper levels (UL) established by FNB and AAC, probably due to environmental contamination. Beyond the high level of some heavy metals found in the Acacia caven pollen, this could be an important dietary supplement by their high Fe and low Na contents. Considering that the suggested doses of commercial pollen by pharmacists and companies advice is 13.5 g/day, an only doses of Acacia caven pollen would satisfy the recommended daily intake (RDI) of Fe given by FNB for an adult individual. In spite of this promising date, further studies on the Fe bioavailability and heavy metal toxicity from Acacia caven pollen should be carried out for being used this pollen as dietary supplement.
Acacia caven (Mol.) Molina pollen causes pollinosis in South America. The aim of this work was to... more Acacia caven (Mol.) Molina pollen causes pollinosis in South America. The aim of this work was to isolate, purify, and characterize the proteolytic enzymes of A. caven pollen, and study their influence on allergy. A series of chromatographic steps were applied to purify the proteolytic extract of A. caven pollen. The purified fraction was partially characterized, and then it was assayed on airway bioactive peptides (substance P, vasoactive intestinal peptide, and bradykinin), and peptide degradation was visualized by direct protein sequencing. The cellular detachment of an airway‐derived epithelial cell line (A‐549) was measured by methylene blue binding assay. The degradation of proteins from intercellular junctions (occludin, claudin, and E‐cadherin) was visualized by Western blot. A 75‐kDa peptidase, named acaciain peptidase, was purified and classified as a serine peptidase. Acaciain peptidase degraded bioactive peptides involved in the maintenance and recovery of the bronchomotor tone; it caused cellular detachment of A‐549 cell line, and degradation of intercellular junction proteins. Acaciain peptidase can alter the integrity of the epithelium barrier, causing cell permeability, increasing the allergic sensitization and exacerbating the overall bronchoconstrictive effect detected in asthmatic lungs. This novel serine peptidase constitutes a relevant therapeutic target in the treatment of allergic disorders.
Growing consumer awareness of the potential negative health effects of synthetic antibiotics has ... more Growing consumer awareness of the potential negative health effects of synthetic antibiotics has prompted the search for more natural preservatives that can improve the safety and quality of food. In this study we report the enzymatic synthesis of N-α-[Carbobenzyloxy]-Ile-Gln (Z-IQ) which is the precursor of Ile-Gln (IQ), a new antibacterial dipeptide, using an aqueous–organic biphasic system formed by 50% (v/v) ethyl acetate in 0.1 M Tris – HCl buffer pH 8. A partially purified proteolytic extract from the fruits of Solanum granuloso leprosum, named granulosain, proved to be a robust biocatalyst for the synthesis of Z-IQ, eliciting 71 ± 0.10% maximal peptide yield in the above described conditions. After cleaving and purifying IQ dipeptide, antimicrobial activity was assayed against Staphylococcus aureus ATCC 25923, Staphylococcus hominis A17771, and Staphylococcus aureus C00195, and MIC values between 118 ± 0.01 μg/mL and 133.7 ± 0.05 μg/mL were obtained. In addition, IQ showed MI...
Colegio de Farmacéuticos de la Provincia de Buenos Aires, Jul 1, 2019
The rapid increase in drug-resistant infections has presented a serious challenge to antimicrobia... more The rapid increase in drug-resistant infections has presented a serious challenge to antimicrobial therapies, encouraging the development of novel control agents. Natural antimicrobial compounds for food have gained attention due to the rise of tolerant microorganisms to food preservation methods. The aim of this work is to evaluate the antibacterial activity of granulosain, the pre-purified proteolytic extract obtained from the fruits of Solanum granuloso-leprosum Dunal, as potential antibacterial compound for the pharmaceutical and food industries. Antibacterial activity of granulosain was tested against representative Gram-positive and Gram-negative strains, in batch culture of Müller-Hinton broth, at 37 ºC and 180 rpm. According to Kruskal-Wallis test, granulosain shows no inhibition of Staphylococcus aureus ATCC 25923, within the studied range. In contrast, granulosain was able to elicit significantly decrease (p ≤ 0.05) on the growth of Escherichia coli ATCC 25922 at protein concentrations equal to or greater than 50 μg/mL.El rápido aumento de las infecciones resistentes a los medicamentos ha presentado un serio desafío para las terapias antimicrobianas, alentando el desarrollo de nuevos agentes de control. Los compuestos antimicrobianos naturales para alimentos han ganado atención debido al aumento de microorganismos tolerantes a los métodos de conservación de alimentos. El objetivo de este trabajo es evaluar la actividad antibacteriana de granulosaína, el extracto proteolítico pre-purificado obtenido de los frutos de Solanum granuloso-leprosum Dunal, como potencial compuesto antibacteriano para las industrias farmacéutica y alimentaria. La actividad antibacteriana de granulosain se probó contra cepas representativas de grampositivos y gramnegativos, en cultivo discontinuo de caldo Müller-Hinton, a 37 ºC y 180 rpm. Según la prueba de Kruskal-Wallis, granulosaína no muestra inhibición de Staphylococcus aureus ATCC 25923, dentro del rango estudiado. Por el contrario, granulosaína fue capaz de provocar una disminución significativa (p ≤ 0.05) en el crecimiento de Escherichia coli ATCC 25922 a concentraciones de proteína iguales o superiores a 50 μg/mL.Fil: Adaro, Mauricio Omar. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Bromatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; ArgentinaFil: Vallés, Diego. Universidad de la República. Facultad de Ciencias; UruguayFil: Cantera, Ana M.. Universidad de la República. Facultad de Ciencias; UruguayFil: Talia, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Barberis, Sonia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Bromatología; Argentin
El incremento de la sensibilidad humana a los alergenos, en particular al polen, tiene relación c... more El incremento de la sensibilidad humana a los alergenos, en particular al polen, tiene relación con factores medioambientales y con el estilo de vida. La cantidad total de polen observado en el aire está creciendo debido a los cambios climáticos. En Europa la floración de muchos árboles y hierbas empieza antes y dura más que hace 10 años, debido a la interacción entre el aumento de la temperatura y la concentración de CO2. Además, los contaminantes químicos del aire pueden modificar las características de los alergenos, entre los que se incluye el polen, aumentando la sensibilidad y las respuestas alérgicas de la población. El tipo de alergia respiratoria más frecuente es causado por la presencia de polen en el aire y se denomina polinosis. Las plantas polinizan durante el día, pero el polen queda en suspensión muchas horas. La concentración de polen en el aire decrece con la distancia, por lo que un solo árbol en un jardín puede tener más repercusiones en la salud que un bosque a 1...
Helicobacter pylori is a gram-negative, helix-shaped, and microaerophilic bacteria that colonizes... more Helicobacter pylori is a gram-negative, helix-shaped, and microaerophilic bacteria that colonizes the human gastric mucosa, causing chronic infections, gastritis, peptic ulcer, lymphomas associated with lymphoid mucosa tissue, and gastric cancer. H. pylori is considered a Type 1 human carcinogen by WHO. The prevalence of the infection is estimated in more than half of the world population. Treatment of H. pylori infection includes antibiotics and proton pump inhibitors, but the increasing antibiotic resistance promotes the research of novel, more effective, and natural antibacterial compounds. The aim of this work was to study the effect of the partially purified proteolytic extract (RAP) of the fruits from Solanum granuloso-leprosum (Dunal), a South American native plant, and a purified fraction named granulosain I, against H. pylori, to obtain natural food additives for the production of anti-H. pylori functional foods. Furthermore, granulosain I and RAP could be used as natural a...
How to cite Complete issue More information about this article Journal's homepage in redalyc.org ... more How to cite Complete issue More information about this article Journal's homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative
SUMMARY. The effect of different organic solvents on the stability of araujiain (the crude enzyme... more SUMMARY. The effect of different organic solvents on the stability of araujiain (the crude enzyme preparation obtained from the latex of fruits of Araujia hortorum Fourn., Asclepiadaceae) in mixtures of buffer and different water-miscible or immiscible organic solvents, and in continuous systems (organic solvents with low water activity) after 4 h at 40 oC was studied in this paper in order to select the most adequate media for peptide enzymatic synthesis. Araujiain showed the highest stability in 50% hexane, 50% propanone, and N,N-dimethylformamide (a w : 0.5 in the enzyme), and those values were remarkably greater than in buffer. In addition, the presence of those organic solvents reduced the autolysis degree. In general, araujiain was not inactivated in aqueous-miscible organic systems and the enzyme showed higher activities in those media than in buffer. In biphasic systems, the partition of the organic solvents into the aqueous phase activated the enzyme in several cases. These...
Antiacanthain and granulosain are the partially purified proteolytic extracts from the South Amer... more Antiacanthain and granulosain are the partially purified proteolytic extracts from the South American native fruits of Bromelia antiacantha (Bertol. ) and Solanum granuloso leprosum, respectively. The aim of this work was to compare the ability of both soluble and immobilized antiacanthain and granulosain f or the synthesis of Z-Tyr-Val-OH, a novel antibacterial dipeptide, in different reaction systems formed by almost anhydrous organic solvents (Xw: 1 × 10−5) and several percentages of immiscible organic solvents in 100 mM Tris(hydroxymethyl)aminomethane hydrochloride buffer pH 8.0. Soluble antiacanthain in half of the 24 different organic biphasic media showed higher catalytic potential than in 100 mM Tris(hydroxymethyl)aminomethane hydrolchloride buffer pH 8.0. Soluble granulosain showed lower catalytic potential in all liquid-liquid biphasic media than in the same buffer. However, 50% (v/v) ethyl ethanoate in 100 mM Tris(hydroxymethyl)aminomethane hydrolchloride buffer pH 8.0 al...
Proteins have functional properties that govern their behavior in foods during processing, storag... more Proteins have functional properties that govern their behavior in foods during processing, storage, and consumption. Proteins can have high nutritional quality and not have functional properties suitable for incorporation into determined food systems. Furthermore, a desirable functional attribute for an additive may be undesirable for everyone else. This chapter describes the design of a new strategy to predict, control, and optimize the functional parameters of food proteins hydrolyzed or not, using chemometrics tools. The starting material consists of proteins whose functional properties are desired to modify. Functional properties (e.g., emulsifying and foaming properties) can be simultaneously evaluated by an experimental statistical design, response surface graphics, and multiple linear regressions. This strategy expands the applications of food proteins and allows the following facilities: assess interactions between variables of multivariate systems, evaluate the dependence b...
Biotechnological Applications of Plant Proteolytic Enzymes
Proteolytic enzymes (proteases) comprise a group of hydrolases (EC 3.4, NC-IUBMB) which share the... more Proteolytic enzymes (proteases) comprise a group of hydrolases (EC 3.4, NC-IUBMB) which share the common feature of acting on peptide bonds. Proteases are among the best-studied enzymes in terms of structure–function relationship (Krowarsch et al. 2005). Proteases, by catalyzing the cleavage of other proteins and even themselves, have an enormous physiological significance, their coding genes representing as much as 2% of the total human genome (Schilling and Overall 2008).
Third International Conference on Advances in Bio-Informatics and Environmental Engineering - ICABEE 2015, Dec 11, 2015
The aim of this work is to study key functional parameters of the goat cheese protein hydrolysate... more The aim of this work is to study key functional parameters of the goat cheese protein hydrolysates. A Plackett-Burman Statistical Design, Response Surface Methodologies and Artificial Neural Network are applied to describe the effects of different factors (pH, Temperature, Time of hydrolysis, Amount of added buffer and Enzyme : Substrate ratio) on the following functional parameters of goat cheese proteins, which are hydrolysed by papain: Free Amine Nitrogen (NA), Total Soluble Nitrogen (NT), Solubility (PSI), Water Holding Capacity (WHC), Emulsifying Activity Index (EAI), Emulsifying Stability Index (ESI), Viscosity (), Held Water (HW), Surface Hydrophobicity (So), Foaming Capacity (FC) and Foam Stability (FS). According to our results, the release of soluble proteins from goat cheese to the supernatant (NT) and the hydrolysis degree of proteins into the supernatant (NA) increased until 443% and 273%, respectively. PSI, WHC, EAI, ESI and HW increased until 443%, 159%, 0.88%, 324% and 64% respectively. decreased until 33% and So (bitter peptides indicator) was reduced until 98.8 %, regard to the original protein isolates. FC and FS were extremely low or null. Predicted values were experimentally confirmed and compared with those of original protein isolates.
Third International Conference on Advances in Bio-Informatics and Environmental Engineering - ICABEE 2015
The aim of this work is to evaluate the mineral and heavy metal contents of Acacia caven (Mol.) M... more The aim of this work is to evaluate the mineral and heavy metal contents of Acacia caven (Mol.) Molina pollen, a South American plant which grows in the Northwest of Argentina, for studying its behavior as bio-indicator of environmental pollution and their consequences as dietary supplement. Mineral and heavy metal contents were determined by flame atomic absorption / emission spectrometry (FAAS and FAES, respectively), inductively coupled plasma optical emission spectrometry (ICP-OES) and hydride generation atomic absorption spectrometry (HG-AAS). The obtained results demonstrated that some mineral contents (K, Ca, P, Fe, Mg, Na, Zn and Se) of Acacia caven pollen met the requirements of both, the Food and Nutritional Board (FNB) and the Argentinean Alimentarius Codex (AAC) to be used as a dietary supplement. Nevertheless, some heavy metals analyzed such as Al, Cu, Ni, V, Cr and Cd, exceeded the upper levels (UL) established by FNB and AAC, probably due to environmental contamination. Beyond the high level of some heavy metals found in the Acacia caven pollen, this could be an important dietary supplement by their high Fe and low Na contents. Considering that the suggested doses of commercial pollen by pharmacists and companies advice is 13.5 g/day, an only doses of Acacia caven pollen would satisfy the recommended daily intake (RDI) of Fe given by FNB for an adult individual. In spite of this promising date, further studies on the Fe bioavailability and heavy metal toxicity from Acacia caven pollen should be carried out for being used this pollen as dietary supplement.
Acacia caven (Mol.) Molina pollen causes pollinosis in South America. The aim of this work was to... more Acacia caven (Mol.) Molina pollen causes pollinosis in South America. The aim of this work was to isolate, purify, and characterize the proteolytic enzymes of A. caven pollen, and study their influence on allergy. A series of chromatographic steps were applied to purify the proteolytic extract of A. caven pollen. The purified fraction was partially characterized, and then it was assayed on airway bioactive peptides (substance P, vasoactive intestinal peptide, and bradykinin), and peptide degradation was visualized by direct protein sequencing. The cellular detachment of an airway‐derived epithelial cell line (A‐549) was measured by methylene blue binding assay. The degradation of proteins from intercellular junctions (occludin, claudin, and E‐cadherin) was visualized by Western blot. A 75‐kDa peptidase, named acaciain peptidase, was purified and classified as a serine peptidase. Acaciain peptidase degraded bioactive peptides involved in the maintenance and recovery of the bronchomotor tone; it caused cellular detachment of A‐549 cell line, and degradation of intercellular junction proteins. Acaciain peptidase can alter the integrity of the epithelium barrier, causing cell permeability, increasing the allergic sensitization and exacerbating the overall bronchoconstrictive effect detected in asthmatic lungs. This novel serine peptidase constitutes a relevant therapeutic target in the treatment of allergic disorders.
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