The National Project is funded by the CASPECO Project which is the Third Phase of the GEF support... more The National Project is funded by the CASPECO Project which is the Third Phase of the GEF supported project in the Caspian Sea region titled " The Caspian Sea: Restoring Depleted Fisheries and Consolidation of a Permanent Regional Environmental Governance Framework " , and implemented by the Iranian Fisheries Research Organization (IFRO) in partnership with four Coastal Research Centers affiliated to IFRO, Coldwater Fishes Research Centre (CFRC).The project's objective is to improve the conservation and restoration of Caspian Salmon (Salmo trutta caspius) stocks. In accordance with IUCN criteria Caspian Salmon is placed in the red list of endangered fish. In the recent decades the destruction of habitats for juveniles, reduction of genetic diversity and fish immune system were main causes of the Caspian salmon decline. Numbers of public meetings were held with participation of fishing communities' representatives, experts, relevant local authorities and experts of ...
The kutum (Rutilus frisii kutum) is one of the economically valuable species of Caspian Sea. Two ... more The kutum (Rutilus frisii kutum) is one of the economically valuable species of Caspian Sea. Two different races of this fish exist in the Caspian Sea, the spring race and the autumn race. The aims of this study were to analysis the population genetic structure and genetic diversity among and between populations of these races based on microsatellite markers. For this purpose, 100 samples of adult kutum from two regions of Caspian Sea (Anzali Wetland and Shiroud River) were collected. DNA was extracted and 31 pair microsatellite primers were used for PCR and 10 made polymorphic patterns. In this study 191 alleles were observed totally. The maximum numbers of alleles (18) were found in two loci (Ca1 and Ca3) and the minimum number of alleles (2) was found in MFW1 locus. The differences between both races were not statistically significant (P>0.05), neither for average number of alleles per locus nor for observed heterozygosities. The calculated Fst and Rst between two races was 0.056 and 0.15 which shows that the genetic difference was significant (P< 0.01). Spring race in MFW1 locus and autumn race in Lid1 locus were at Hardy-Weinberg equation but not in other loci in both races. The genetic distance was 0.407 which indicating the greatest genetic distance between the two studied races. The data generated in this study showed that the spring and autumn races of kutum in two region of southern part of Caspian Sea are two independent populations.
In conformity with the sex determination of Gracilaria corticata in the Persian Gulf and Oman Sea... more In conformity with the sex determination of Gracilaria corticata in the Persian Gulf and Oman Sea, a total of 41 samples were collected from two stations of Bostaneh region (northern Persian Gulf, 54° 38´ E / 26° 30´ N) and Lipar regions (northern Oman Sea, 60° 49´ E / 25° 15´ N). The specimens were cultured in PES media for observing the different life stages. The anatomical structures of thallus were taken into consideration. The diploid tetrasporophytes and spermatangia in thallus of male’s gametophytes; and Carpospore and cystocarps of female were determined. Due to DNA extraction, the parasites and epiphytes were cleaned and then the under growing sections were sectioned using liquid nitrogen. After extraction of DNA, by using 20 different primers according to ISSR molecular indicator, the sex diversity and genetic diversity of populations were studied; and four primers were selected ultimately. The obtained results were analyzed by GenAlex and PopGen softwares. In total, 74 ba...
In this article, based on mutual appropriation of concepts from cultural-historical activity theo... more In this article, based on mutual appropriation of concepts from cultural-historical activity theory (especially Andy Blunden’s works) and Iranian Marxist tradition (especially Kamal Khosravi’s ideas), I have elaborated two crises which, we believe, are main characteristics of our historical stage and other crises are manifestations of them: (a) crisis of historical perspective on human self-emancipation and (b) crisis of theory in emancipatory social science. Crisis of historical perspective indicates lack of integrated worldview and concept about emancipated future, while crisis of theory addresses the fundamental problem of theoretical categories in expressing relations between elements and factors of reality. Categories of mode of existence (Marx, Khosravi) and mode of collaboration (Blunden) are proposed in order to solve these crises. Based on the mutual appropriation of mode of existence and mode of collaboration, I have tried to formulate three modes of existence of projects ...
Go to AGRIS search. Try it! An Ecological investigating on controling Mnemiopsis leidyi populatio... more Go to AGRIS search. Try it! An Ecological investigating on controling Mnemiopsis leidyi population Activity 6: Molecular Test in identification of Menmiopsis leidyi. ...
ABSTRACT This investigation was conducted in order to find the etiological factors of fry mortali... more ABSTRACT This investigation was conducted in order to find the etiological factors of fry mortality syndrome that causes notifiable economical lost in rainbow trout fish farms. During 43 months, from Sep. 1999 until May 2002, 52 fish farms and hatcheries which were located in Tehran, Mazandaran, Guilan, Fras, Markazi, Kerman and kohgiloyeh and boyerahmad provinces, were visited and samples from suspected fishes were obtained. The samples were consist of ovarian fluids and ovule, milt, egg, eyed-egg, larvae, fry less than I g and at 1-3 g as well as adult fish consist of internal organs. From these collected samples. 410 tissue sample, 899 sample, 55 Tissue sample, 200 larvae sample, 56 blood serum sample, 50 whole blood sample, 104 Tissue sample and 55 samples were used for Virological, Bacteriological, Electron Microscope assay, DF A T) , ELISA, Hematological, Histopathological and PCR assay, respectively. The clinical signs of suspected fishes were color darkness, exophthalmia, ascites, whirling and abnormal swimming. No cytopathic effects (CPE) were observed after inoculation of suspected samples on the cell lines ofBF-2, FHM, and EPC. A number of some samples of 56 blood serum samples were identified as positive sample for IHN, VHS and IPN virus. Optical Density (OD) of positive samples was 7-9 times more than blank samples. Regarding to the result of this study, it could be indicated that the causative agents for fry mortality syndrome may be IHN, VHS and IPN viruses. Further investigation using PCR for definitive diagnosis and confirmation of the causative agents would be recommended.
Lactococcosis caused by Lactococcus garvieae is known as one of the serious economical bacterial ... more Lactococcosis caused by Lactococcus garvieae is known as one of the serious economical bacterial diseases in aquaculture species of both freshwater and marine fish at a wide range of water temperature mainly from 13°C to 37°C. The PavA gene and Hly gene, the genes responsible for encoding fibronectin and hemolysin proteins, respectively are the well known virulent factors of L. garvieae. The effect of essential oil of Zataria multiflora was evaluated on expression of these genes in two virulent isolates of L. garvieae isolates recovered from diseased rainbow trout. Fresh cultures of the bacterial isolates were exposed to 1/2-1/16 (0.12-0.015 μl/ml) of the minimum inhibitory concentrations (MIC) of Z. multiflora essential oil and the expression of PavA and Hly genes was assessed by real time PCR works. The results showed that under MIC concentration (0.12 μl/ml) of the essential oil, the expression of both Hly and PavA genes was significantly suppressed in both bacterial isolates (P b 0.05). Therefore, it is feasible to use Z. multiflora in forms of oral and/or bath administration to reduce morbidity and mortality by Lactococcus garvieae in fish via the suppression of expression of these virulent genes.
Journal of Experimental Marine Biology and Ecology, 2006
This study concentrated on the mitochondrial DNA diversity in adult Artemia urmiana populations. ... more This study concentrated on the mitochondrial DNA diversity in adult Artemia urmiana populations. A total of 210 individual specimens were collected from the surface and bottom layers from three different geographical areas, comprising six sampling sites in Urmia ...
The objective of this investigation was molecular study of Penaeus indicus population from Jask a... more The objective of this investigation was molecular study of Penaeus indicus population from Jask area. Samples were collected using bottem trawl net from three regions, East, West and Central Jask. DNA were extracted using phenol - chloroform method from muscular tissue. To ...
The National Project is funded by the CASPECO Project which is the Third Phase of the GEF support... more The National Project is funded by the CASPECO Project which is the Third Phase of the GEF supported project in the Caspian Sea region titled " The Caspian Sea: Restoring Depleted Fisheries and Consolidation of a Permanent Regional Environmental Governance Framework " , and implemented by the Iranian Fisheries Research Organization (IFRO) in partnership with four Coastal Research Centers affiliated to IFRO, Coldwater Fishes Research Centre (CFRC).The project's objective is to improve the conservation and restoration of Caspian Salmon (Salmo trutta caspius) stocks. In accordance with IUCN criteria Caspian Salmon is placed in the red list of endangered fish. In the recent decades the destruction of habitats for juveniles, reduction of genetic diversity and fish immune system were main causes of the Caspian salmon decline. Numbers of public meetings were held with participation of fishing communities' representatives, experts, relevant local authorities and experts of ...
The kutum (Rutilus frisii kutum) is one of the economically valuable species of Caspian Sea. Two ... more The kutum (Rutilus frisii kutum) is one of the economically valuable species of Caspian Sea. Two different races of this fish exist in the Caspian Sea, the spring race and the autumn race. The aims of this study were to analysis the population genetic structure and genetic diversity among and between populations of these races based on microsatellite markers. For this purpose, 100 samples of adult kutum from two regions of Caspian Sea (Anzali Wetland and Shiroud River) were collected. DNA was extracted and 31 pair microsatellite primers were used for PCR and 10 made polymorphic patterns. In this study 191 alleles were observed totally. The maximum numbers of alleles (18) were found in two loci (Ca1 and Ca3) and the minimum number of alleles (2) was found in MFW1 locus. The differences between both races were not statistically significant (P>0.05), neither for average number of alleles per locus nor for observed heterozygosities. The calculated Fst and Rst between two races was 0.056 and 0.15 which shows that the genetic difference was significant (P< 0.01). Spring race in MFW1 locus and autumn race in Lid1 locus were at Hardy-Weinberg equation but not in other loci in both races. The genetic distance was 0.407 which indicating the greatest genetic distance between the two studied races. The data generated in this study showed that the spring and autumn races of kutum in two region of southern part of Caspian Sea are two independent populations.
In conformity with the sex determination of Gracilaria corticata in the Persian Gulf and Oman Sea... more In conformity with the sex determination of Gracilaria corticata in the Persian Gulf and Oman Sea, a total of 41 samples were collected from two stations of Bostaneh region (northern Persian Gulf, 54° 38´ E / 26° 30´ N) and Lipar regions (northern Oman Sea, 60° 49´ E / 25° 15´ N). The specimens were cultured in PES media for observing the different life stages. The anatomical structures of thallus were taken into consideration. The diploid tetrasporophytes and spermatangia in thallus of male’s gametophytes; and Carpospore and cystocarps of female were determined. Due to DNA extraction, the parasites and epiphytes were cleaned and then the under growing sections were sectioned using liquid nitrogen. After extraction of DNA, by using 20 different primers according to ISSR molecular indicator, the sex diversity and genetic diversity of populations were studied; and four primers were selected ultimately. The obtained results were analyzed by GenAlex and PopGen softwares. In total, 74 ba...
In this article, based on mutual appropriation of concepts from cultural-historical activity theo... more In this article, based on mutual appropriation of concepts from cultural-historical activity theory (especially Andy Blunden’s works) and Iranian Marxist tradition (especially Kamal Khosravi’s ideas), I have elaborated two crises which, we believe, are main characteristics of our historical stage and other crises are manifestations of them: (a) crisis of historical perspective on human self-emancipation and (b) crisis of theory in emancipatory social science. Crisis of historical perspective indicates lack of integrated worldview and concept about emancipated future, while crisis of theory addresses the fundamental problem of theoretical categories in expressing relations between elements and factors of reality. Categories of mode of existence (Marx, Khosravi) and mode of collaboration (Blunden) are proposed in order to solve these crises. Based on the mutual appropriation of mode of existence and mode of collaboration, I have tried to formulate three modes of existence of projects ...
Go to AGRIS search. Try it! An Ecological investigating on controling Mnemiopsis leidyi populatio... more Go to AGRIS search. Try it! An Ecological investigating on controling Mnemiopsis leidyi population Activity 6: Molecular Test in identification of Menmiopsis leidyi. ...
ABSTRACT This investigation was conducted in order to find the etiological factors of fry mortali... more ABSTRACT This investigation was conducted in order to find the etiological factors of fry mortality syndrome that causes notifiable economical lost in rainbow trout fish farms. During 43 months, from Sep. 1999 until May 2002, 52 fish farms and hatcheries which were located in Tehran, Mazandaran, Guilan, Fras, Markazi, Kerman and kohgiloyeh and boyerahmad provinces, were visited and samples from suspected fishes were obtained. The samples were consist of ovarian fluids and ovule, milt, egg, eyed-egg, larvae, fry less than I g and at 1-3 g as well as adult fish consist of internal organs. From these collected samples. 410 tissue sample, 899 sample, 55 Tissue sample, 200 larvae sample, 56 blood serum sample, 50 whole blood sample, 104 Tissue sample and 55 samples were used for Virological, Bacteriological, Electron Microscope assay, DF A T) , ELISA, Hematological, Histopathological and PCR assay, respectively. The clinical signs of suspected fishes were color darkness, exophthalmia, ascites, whirling and abnormal swimming. No cytopathic effects (CPE) were observed after inoculation of suspected samples on the cell lines ofBF-2, FHM, and EPC. A number of some samples of 56 blood serum samples were identified as positive sample for IHN, VHS and IPN virus. Optical Density (OD) of positive samples was 7-9 times more than blank samples. Regarding to the result of this study, it could be indicated that the causative agents for fry mortality syndrome may be IHN, VHS and IPN viruses. Further investigation using PCR for definitive diagnosis and confirmation of the causative agents would be recommended.
Lactococcosis caused by Lactococcus garvieae is known as one of the serious economical bacterial ... more Lactococcosis caused by Lactococcus garvieae is known as one of the serious economical bacterial diseases in aquaculture species of both freshwater and marine fish at a wide range of water temperature mainly from 13°C to 37°C. The PavA gene and Hly gene, the genes responsible for encoding fibronectin and hemolysin proteins, respectively are the well known virulent factors of L. garvieae. The effect of essential oil of Zataria multiflora was evaluated on expression of these genes in two virulent isolates of L. garvieae isolates recovered from diseased rainbow trout. Fresh cultures of the bacterial isolates were exposed to 1/2-1/16 (0.12-0.015 μl/ml) of the minimum inhibitory concentrations (MIC) of Z. multiflora essential oil and the expression of PavA and Hly genes was assessed by real time PCR works. The results showed that under MIC concentration (0.12 μl/ml) of the essential oil, the expression of both Hly and PavA genes was significantly suppressed in both bacterial isolates (P b 0.05). Therefore, it is feasible to use Z. multiflora in forms of oral and/or bath administration to reduce morbidity and mortality by Lactococcus garvieae in fish via the suppression of expression of these virulent genes.
Journal of Experimental Marine Biology and Ecology, 2006
This study concentrated on the mitochondrial DNA diversity in adult Artemia urmiana populations. ... more This study concentrated on the mitochondrial DNA diversity in adult Artemia urmiana populations. A total of 210 individual specimens were collected from the surface and bottom layers from three different geographical areas, comprising six sampling sites in Urmia ...
The objective of this investigation was molecular study of Penaeus indicus population from Jask a... more The objective of this investigation was molecular study of Penaeus indicus population from Jask area. Samples were collected using bottem trawl net from three regions, East, West and Central Jask. DNA were extracted using phenol - chloroform method from muscular tissue. To ...
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