This content is licensed under a Creative Commons Attributions 4.0 International License. Compari... more This content is licensed under a Creative Commons Attributions 4.0 International License. Comparison of strip meniscometry and Schirmer tear test results and tear film breakup time between healthy dogs and dogs with dry eye disease Comparação entre o teste de meniscometria em tira, o teste lacrimal de Schirmer e o tempo de ruptura do filme lacrimal em cães normais e com olho seco
The objective of this study was to evaluate the use of the Kowa HA-2 applanation tonometer in mea... more The objective of this study was to evaluate the use of the Kowa HA-2 applanation tonometer in measuring intraocular pressure (IOP) in cats. Ten healthy eyes were used in an ex vivo study in which the calibration curve for manometry vs. tonometry was determined by artificially raising the IOP in 5 mmHg increments up to 60 mmHg (10-60 mmHg). Both eyes of 10 anesthetized cats were studiedin vivo to compare manometry vs. tonometry. In the ambulatory study, 78 healthy eyes, 7 eyes with glaucoma and 20 eyes with uveitis were evaluated by tonometry, which was performed with topical anesthesia and 1% fluorescein eye drops for the formation of fluorescein semicircles. The correlation coefficient (r²) between the manometer and the Kowa HA-2 tonometer was 0.993 and the linear regression equation was y = 0.0915x + 0.0878 in the ex-vivo study. In the in vivo study, the IOP values (mean±SD, in mmHg) in manometry were 15.6 ± 1.1(14.0-17.5) and in tonometry were 15.5 ± 1.2(13.5-17.2), with no significant difference (P > 0.05) between manometry and tonometry. In ambulatory study, using the Kowa HA-2 tonometer, the IOP values (mean±SD, in mmHg) were 15.0 ± 1.5 (11.8-18.3) for the healthy eyes, 38.4 ± 8.1(29.6-53.7) for glaucomatous eyes and 10.4 ± 2.0(5.3-12.2) for eyes with uveitis. There was a strong correlation and accuracy between the IOP values with the manometry and the Kowa HA-2 tonometer. In the ambulatorystudy the IOP values obtained with the tonometer were compatible for animals with healthy eyes and with clinical signs of glaucoma and uveitis. We conclude that the Kowa HA-2 tonometer can be used in the measurement of IOP in cats, since it is a practical and accurate method in this species.
The objective was to perform ocular biometry and ophthalmic examination in dogs of the French Bul... more The objective was to perform ocular biometry and ophthalmic examination in dogs of the French Bulldog breed. We evaluated 72 normal eyes of 36 healthy French Bulldog dogs, 20 males and 16 females, aged from 1 to 7 years. Ocular ultrasound was performed in B mode with a 10 MHz linear probe, and the following parameters of ocular biometry were evaluated: horizontal axial diameter (HAD) of the eye, lens thickness (LT), anterior chamber depth (ACD), and vitreous chamber depth (VCD). The ophthalmic exams performed were as follows: Schirmer Tear Test-1 (STT-1), rebound tonometry (Tonovet), Tear Film Break-up Time (TBUT), and Fluorescein Test (FT). The ocular biometry results (mean±SD) in millimeters were: 20.5 ± 0.6 (HAD), 3.8 ± 0.4 (ACL), 7.4 ± 0.3 (LT) and 9.4 ± 0.4 (VCL), and from the ophthalmic exams (mean±SD) were: 24 ± 3.7 (16.5-33.5) mm/min (STT-1), 20.0 ± 3.7 (13.5-27.0) mmHg (rebound tonometry), and 24.4 ± 6.3 (16-40) seconds (TBUT), and FT was negative in all dogs. The obtained results can serve as parameters of normal values of ocular biometry and ophthalmic exams for ambulatory routines, intraocular surgeries, and future ophthalmic studies in this breed.
RESUMO É denominado fecaloma quando as fezes estão retidas e ressecadas no interior do colón. O p... more RESUMO É denominado fecaloma quando as fezes estão retidas e ressecadas no interior do colón. O paciente pode apresentar sinais clínicos como dificuldade de defecar, vômitos, constipação, apatia, tenesmo e hematoquesia. Ocorrem com maior incidência em felinos devida seus hábitos comportamentais de higiene, principalmente pela ingestão de pelos e também pela recusa de defecação ao encontrar o local sujo. Outro fator desencadeante é a baixa ingestão de agua. O presente estudo tem por objetivo relatar o caso de um fecaloma grave em um felino macho, sem raça definida, pelagem preta, quatro anos de idade. O paciente foi submetido ao procedimento cirúrgico de enterotomia e após sete meses retornou ao hospital veterinário da UNOESTE com recidiva do quadro, onde foi realizado novamente o procedimento anterior. Neste caso, foi recomendado alimentação pastosa por toda a vida, devido a sua predisposição ao fecaloma. Palavras-chave: constipação; felino; megacolon.
Keratoconjunctivitis sicca (KCS) is of predominantly immune-mediated origin. Dogs are an excellen... more Keratoconjunctivitis sicca (KCS) is of predominantly immune-mediated origin. Dogs are an excellent model for understanding this disease, as the origin of KCS in dogs is like that in humans. The objective of this study was to localize and quantify immunological markers, such as CD4 lymphocytes, interleukin (IL)-1, IL-6 and tumor necrosis factor alpha (TNFα), before and after topical treatment with mesenchymal stem cells (MSCs). Twentytwo dogs positive for KCS were topically treated with 50 μL (1 × 10 6 MSCs) in the conjunctival sac and were evaluated for 6 months. The levels of the markers CD4, IL-6, IL-1 and TNFα were analyzed in conjunctival biopsy and cytology of the third eyelid gland by immunohistochemistry and immunocytochemistry. The results showed that before treatment, there was marked expression of all the markers (CD4, IL-6, IL-1 and TNFα), and after 6 months, there were significant (p < .05) reductions in the expression levels of all the markers. These results demonstrated that topical MSC treatment promotes a significant decrease in the expression levels of these inflammatory markers and could be used as adjuvant therapy in the treatment of KCS in dogs and humans. In addition, these markers can be excellent tools for diagnosing and analyzing the progression of KCS.
Resveratrol has antioxidant, anti-inflammatory, lipolytic, and antifibrotic properties, which may... more Resveratrol has antioxidant, anti-inflammatory, lipolytic, and antifibrotic properties, which may be useful in supplementation of obese patients and with liver problems. This study evaluated the effects of 6-week resveratrol supplementation on the lipid profile and liver function of female Wistar rats fed a high-fat diet to induce obesity. Sixty-four Wistar rats were divided into 4 groups (n = 16): the control group (C); the control obese group (CO); the resveratrol group (R); and the resveratrol obese group (RO). At the end of the experiment, the animals were anesthetized for blood collection and subsequent euthanasia for collection of liver biopsy. The parameters for body weight, liver weight, retroperitoneal fat weight, serum lipid and liver profiles and histopathological analysis were evaluated. The 6-week resveratrol administration did not induce weight loss nor did it reduce the lipid profile; however, it decreased the liver enzymes aspartate aminotransferase (AST) and alkalin...
Brachycephalic dogs have a normal lower jaw and a receded upper jaw, and these dogs are predispos... more Brachycephalic dogs have a normal lower jaw and a receded upper jaw, and these dogs are predisposed to nostril stenosis, elongated palate, tracheal hypoplasia and hyperthermia due to heat stress. Given these characteristics, these dogs are more predisposed to respiratory and cardiovascular disorders. The popularity of brachycephalic dogs that have a greater tendency for cardiorespiratory disorders, such as French bulldogs, has increased recently; however, studies on the cardiorespiratory and haematological parameters in this breed are lacking. Thus, the present study sought to evaluate the cardiorespiratory and haematological parameters of healthy French bulldogs to standardize normal values for this breed. Thirtysix French bulldogs were used without predilection for age or sex, and the following parameters were evaluated: temperature (T), heart rate (HR), respiratory rate (RR), erythrogram, leukogram, total plasma protein, plasmatic fibrinogen, blood platelet count, urea, creatinine, alkaline phosphatase (AP) and alanine aminotransferase (ALT). The mean and standard deviation were as follows: T°C (38.3±0.4), HR (111.1±27.0) beats per minute, RR (54.8±32.5) breaths per minute, erythrocyte count (7.3±1.2x10 6), haemoglobin (16.9±2.8 g/dL), haematocrit (48.9±7.7%), MCV (67.6±2.4 fL), MCH (23.3±0.7 pg), MCHC (34.6±0.8%), RDW-CV (9.3±0.9; 9.2-12.0%), total leukocytes (11009.7±2907.3/mm 3), band neutrophils (250.0±260.4/mm 3), segmented neutrophils (7094.4±2307.3/mm 3), eosinophils (589.7±561.3/mm 3), lymphocytes (2020.8±1457.5/mm 3), monocytes (987.6±528.4/mm 3), total plasma protein (6.9±0.5 g/dL), plasmatic fibrinogen (274.1±96.5 mg/dL), blood platelet count (366.4±88.5/mm 3), urea (35.2±12.6 mg/dL), creatinine (0.8±0.2/mg/dL), AP (49.1±25.8U/L), ALT (36.2±12.3U/L). The haematological parameters and heart rate were within the normal values for the species, but the respiratory rate values (54.8±32.5 breaths per minute) were greater than the normal average of 10 to 30 breaths per minute. We conclude that the haematological parameters and heart rate of healthy French bulldogs are within the normal limit for dogs; however, the respiratory rate is above reference values, reinforcing the notion that dogs of this breed, although healthy, exhibit an increased risk of respiratory disorders.
Background and Aim: Keratoconjunctivitis sicca (KCS) is predominantly an immune-mediated chronic ... more Background and Aim: Keratoconjunctivitis sicca (KCS) is predominantly an immune-mediated chronic inflammatory ocular disease that is commonly diagnosed in dogs. This study aimed to compare the conventional use of topical immunosuppressant tacrolimus 0.03% eye drops and a new therapy injectable homologous platelet-rich plasma (HPRP) into the third eyelid gland and inferior and superior palpebral conjunctiva of dogs with KCS. Materials and Methods: A total of 66 eyes from 33 dogs were evaluated. The eyes were divided into three equal groups: Negative control group, tacrolimus group (TG), and homologous platelet-rich plasma group (HPRPG). The animals were evaluated using the Schirmer's tear test-1 (STT-1), osmolarity test (OT), strip meniscometry test (SMT), tear film break-up test (TBUT), fluorescein test, lissamine green test (LGT), and cytological and histopathological analyses. Results: In TG, there was a significant increase (p < 0.05) in the STT-1 and SMT values, and goble...
Background and Aim: Tonometers are an important instrument for measuring intraocular pressure (IO... more Background and Aim: Tonometers are an important instrument for measuring intraocular pressure (IOP) in the diagnosis of glaucoma or uveitis. This study aimed to compare the accuracy of the main types of tonometers with different IOP measurement methodologies in dogs: TonoVet and TonoVet Plus (rebound), Tono-Pen Avia Vet (applanation), and Kowa HA-2 (Goldmann applanation). Materials and Methods: IOP was measured in 152 eyes of 76 dogs. A postmortem study was performed by comparing manometry and tonometry values and calculating the correlation coefficient (r2), in vivo real IOP (manometry) among the tonometers was compared, and an outpatient study was conducted with healthy eyes and eyes with signs of glaucoma and uveitis. Results: In the postmortem study, the values of r2 in descending order were Kowa (0.989), TonoVet Plus (0.984), TonoVet (0.981), and Tono-Pen Avia Vet (0.847). The IOP values in mmHg in the in vivo study were as follows: Aneroid manometer (16.8±2.5.7), TonoVet (18.1...
The objective of this study was to compare the efficacy of two sources of omega 3 and 6, fish oil... more The objective of this study was to compare the efficacy of two sources of omega 3 and 6, fish oil (FO) and linseed oil (LO), orally administered, alone or in combination, for treating experimentally induced keratoconjunctivitis sicca (KCS) in rabbits. Twenty-eight New Zealand rabbits were used in this study. Seven animals were allocated to the C group (negative control), and KCS was induced in 21 animals by topically applying 1% atropine sulfate drops for 7 days. Treatment with atropine was maintained throughout the study period (12 weeks). The rabbits were divided into 3 treatment groups containing 7 animals each: FO group, LO group and FLO group (FO and LO). The animals were evaluated using the Schirmer Tear Test I (STT I), Rose Bengal Test (RBT), fluorescein test (FT), tear film break-up time (TBUT), and conjunctival and histopathological analysis. There was a significant increase in STT I and TBUT values in treatment groups, but the increase occurred earlier in the FO group. The...
To evaluate the effectiveness of topical 1% cyclosporine eye drops diluted in either of the two v... more To evaluate the effectiveness of topical 1% cyclosporine eye drops diluted in either of the two vehicles-olive and linseed oil-and that of the oils themselves in treating experimentally-induced keratoconjunctivitis sicca (KCS) in rabbits. KCS was induced in 25 New Zealand rabbits using 1% atropine sulfate eye drops for 7 days before treatment and throughout the treatment period (12 weeks). The rabbits were divided into five groups: one control (C) group without KCS induction and four treatment groups in which KCS was induced and treated topically with olive oil (O), linseed oil (L), cyclosporine in olive oil (CO), and cyclosporine in linseed oil (CL). The animals were evaluated using Schirmer tear test 1 (STT), the fluorescein test (FT), tear-film break-up time (TBUT), the rose bengal test (RBT), and histopathological analysis. Values of STT and TBUT significantly decreased 1 week post-induction (p<0.05) and were similar to initial values after the 4th week of treatment, in all g...
Animais do HV da UNOESTE. 3 Residente do Laboratório Clínico do HV da UNOESTE RESUMO A anemia hem... more Animais do HV da UNOESTE. 3 Residente do Laboratório Clínico do HV da UNOESTE RESUMO A anemia hemolítica imunomediada (AHIM) é definida como uma redução do número de eritrócitos em decorrência da destruição dos mesmos pelo sistema imune devido à presença de imunoglobulinas (IgM e IgG) na membrana do eritrócito. Foram atendidos dois casos desta enfermidade, os quais foram diagnosticados pela somatória historia clínica, resultados dos exames e evolução positiva mediante tratamento clínico efetivo. Esse relato tem por objetivo fazer uma breve revisão do assunto e contribuir com mais dados sobre essa doença. Palavras-chave: Anemia imunomediada. Esferócitos. Hemólise. Cães.
Journal of Ophthalmic Inflammation and Infection, 2013
Background The objective of this study was to evaluate the effectiveness of various linseed oil (... more Background The objective of this study was to evaluate the effectiveness of various linseed oil (LO) preparations (oral, topical, oral and topical combined) in treating experimentally induced keratoconjunctivitis sicca (KCS) in rabbits. Twenty male New Zealand white rabbits were divided into four groups: group C (control), group OLO (oral LO), group TLO (topical LO), and group OTLO (oral and topical LO). The animals were evaluated weekly using Schirmer's tear test (STT), fluorescein test (FT), and Rose Bengal test (RBT) and were euthanized at the end of the experiment for histopathological analysis. Findings There were significant improvements in the parameters analyzed (STT, FT, and RBT) and in the histopathological finding in all of the groups using LO. Conclusions The analyzed results demonstrate that LO, administered orally or topically, was effective in treating experimentally induced KCS in rabbits, although combined oral and topical LO did not show additional benefits gre...
To compare the efficacy of 0.03% tacrolimus eye drops diluted in two different vehicles (linseed ... more To compare the efficacy of 0.03% tacrolimus eye drops diluted in two different vehicles (linseed oil and olive oil) for the treatment of keratoconjunctivitis sicca (KCS) in dogs. This study included 60 dogs. Of this group, 20 were healthy and allocated to the control group, and 40 were diagnosed with bilateral KCS and randomly allocated to either the TO (tacrolimus in olive oil) or the TL (tacrolimus in linseed oil) groups. Ophthalmic examinations, Schirmer Tear Test-1 (STT-1), Tear Film Break-up Time (TBUT) and Fluorescein Test (FT) were carried out monthly, along with cytological and histopathological examinations at the beginning and end of the study. The clinical signs, corneal ulcers, Schirmer Tear Test-1 values, and Tear Film Break-up Time values improved in both groups after one month of treatment. Cytological examination at the end of the study showed decreased lymphocytes, neutrophil, metaplastic, and squamous cell counts in both groups, while the histopathological analysis...
Background and Aim: Keratoconjunctivitis sicca (KCS) is predominantly an immune-mediated chronic ... more Background and Aim: Keratoconjunctivitis sicca (KCS) is predominantly an immune-mediated chronic inflammatory ocular disease that is commonly diagnosed in dogs. This study aimed to compare the conventional use of topical immunosuppressant tacrolimus 0.03% eye drops and a new therapy injectable homologous platelet-rich plasma (HPRP) into the third eyelid gland and inferior and superior palpebral conjunctiva of dogs with KCS.
Materials and Methods: A total of 66 eyes from 33 dogs were evaluated. The eyes were divided into three equal groups: Negative control group, tacrolimus group (TG), and homologous platelet-rich plasma group (HPRPG). The animals were evaluated using the Schirmer's tear test-1 (STT-1), osmolarity test (OT), strip meniscometry test (SMT), tear film break-up test (TBUT), fluorescein test, lissamine green test (LGT), and cytological and histopathological analyses.
Results: In TG, there was a significant increase (p < 0.05) in the STT-1 and SMT values, and goblet cell count in the palpebral conjunctiva by the end of the study. In HPRPG, 36% (four dogs) received three applications, 55% (six dogs) received two applications, and 9% (one dog) received one application before the initial ocular signs improved. There was a significant decrease (p < 0.05) in the lymphocyte and neutrophil counts of the palpebral conjunctiva in HPRPG than in TG. Both groups showed equivalent improvements in TBUT, OT, and LGT values.
Conclusion: Tacrolimus 0.03% eye drops were more efficient than HPRP in increasing tear production and the number of goblet cells. However, injectable HPRP was more efficient than tacrolimus in decreasing the number of conjunctival inflammatory cells. Treatment with injectable HPRP requires an average of two to three applications, is safe and feasible, and can be used as a cheaper alternative or as an adjuvant to conventional treatment with topical immunosuppressants.
Brazilian Journal of Veterinary Research and Animal Science, 2008
O objetivo do presente trabalho foi estudar as características em comum e as diferenças observada... more O objetivo do presente trabalho foi estudar as características em comum e as diferenças observadas na intoxicação induzida por 1 mg/kg de amitraz, IV, em cães e gatos. Os principais sinais clínicos observados em comum foram sedação, hipotermia, bradicardia, bradiarritmias, hipotensão, bradipnéia, midríase e hiperglicemia transitória, porém a intensidade destes sinais foi diferente entre as espécies. A hipotermia foi mais acentuada em gatos. Os cães foram mais sensíveis às alterações cardiorespiratórias apresentando diminuição mais significativa na freqüência cardíaca e respiratória, além de ocorrência de maior número de bradiarritmias. Os gatos apresentaram midríase mais prolongada do que os cães. Observou-se hiperglicemia e hipoinsulinemia transitórias e diminuição transitória dos níveis plasmáticos de cortisol em ambas espécies, porém os gatos apresentaram um pico de hiperglicemia maior e mais precoce do que os cães, e com relação aos níveis plasmáticos de cortisol, os cães aprese...
This content is licensed under a Creative Commons Attributions 4.0 International License. Compari... more This content is licensed under a Creative Commons Attributions 4.0 International License. Comparison of strip meniscometry and Schirmer tear test results and tear film breakup time between healthy dogs and dogs with dry eye disease Comparação entre o teste de meniscometria em tira, o teste lacrimal de Schirmer e o tempo de ruptura do filme lacrimal em cães normais e com olho seco
The objective of this study was to evaluate the use of the Kowa HA-2 applanation tonometer in mea... more The objective of this study was to evaluate the use of the Kowa HA-2 applanation tonometer in measuring intraocular pressure (IOP) in cats. Ten healthy eyes were used in an ex vivo study in which the calibration curve for manometry vs. tonometry was determined by artificially raising the IOP in 5 mmHg increments up to 60 mmHg (10-60 mmHg). Both eyes of 10 anesthetized cats were studiedin vivo to compare manometry vs. tonometry. In the ambulatory study, 78 healthy eyes, 7 eyes with glaucoma and 20 eyes with uveitis were evaluated by tonometry, which was performed with topical anesthesia and 1% fluorescein eye drops for the formation of fluorescein semicircles. The correlation coefficient (r²) between the manometer and the Kowa HA-2 tonometer was 0.993 and the linear regression equation was y = 0.0915x + 0.0878 in the ex-vivo study. In the in vivo study, the IOP values (mean±SD, in mmHg) in manometry were 15.6 ± 1.1(14.0-17.5) and in tonometry were 15.5 ± 1.2(13.5-17.2), with no significant difference (P > 0.05) between manometry and tonometry. In ambulatory study, using the Kowa HA-2 tonometer, the IOP values (mean±SD, in mmHg) were 15.0 ± 1.5 (11.8-18.3) for the healthy eyes, 38.4 ± 8.1(29.6-53.7) for glaucomatous eyes and 10.4 ± 2.0(5.3-12.2) for eyes with uveitis. There was a strong correlation and accuracy between the IOP values with the manometry and the Kowa HA-2 tonometer. In the ambulatorystudy the IOP values obtained with the tonometer were compatible for animals with healthy eyes and with clinical signs of glaucoma and uveitis. We conclude that the Kowa HA-2 tonometer can be used in the measurement of IOP in cats, since it is a practical and accurate method in this species.
The objective was to perform ocular biometry and ophthalmic examination in dogs of the French Bul... more The objective was to perform ocular biometry and ophthalmic examination in dogs of the French Bulldog breed. We evaluated 72 normal eyes of 36 healthy French Bulldog dogs, 20 males and 16 females, aged from 1 to 7 years. Ocular ultrasound was performed in B mode with a 10 MHz linear probe, and the following parameters of ocular biometry were evaluated: horizontal axial diameter (HAD) of the eye, lens thickness (LT), anterior chamber depth (ACD), and vitreous chamber depth (VCD). The ophthalmic exams performed were as follows: Schirmer Tear Test-1 (STT-1), rebound tonometry (Tonovet), Tear Film Break-up Time (TBUT), and Fluorescein Test (FT). The ocular biometry results (mean±SD) in millimeters were: 20.5 ± 0.6 (HAD), 3.8 ± 0.4 (ACL), 7.4 ± 0.3 (LT) and 9.4 ± 0.4 (VCL), and from the ophthalmic exams (mean±SD) were: 24 ± 3.7 (16.5-33.5) mm/min (STT-1), 20.0 ± 3.7 (13.5-27.0) mmHg (rebound tonometry), and 24.4 ± 6.3 (16-40) seconds (TBUT), and FT was negative in all dogs. The obtained results can serve as parameters of normal values of ocular biometry and ophthalmic exams for ambulatory routines, intraocular surgeries, and future ophthalmic studies in this breed.
RESUMO É denominado fecaloma quando as fezes estão retidas e ressecadas no interior do colón. O p... more RESUMO É denominado fecaloma quando as fezes estão retidas e ressecadas no interior do colón. O paciente pode apresentar sinais clínicos como dificuldade de defecar, vômitos, constipação, apatia, tenesmo e hematoquesia. Ocorrem com maior incidência em felinos devida seus hábitos comportamentais de higiene, principalmente pela ingestão de pelos e também pela recusa de defecação ao encontrar o local sujo. Outro fator desencadeante é a baixa ingestão de agua. O presente estudo tem por objetivo relatar o caso de um fecaloma grave em um felino macho, sem raça definida, pelagem preta, quatro anos de idade. O paciente foi submetido ao procedimento cirúrgico de enterotomia e após sete meses retornou ao hospital veterinário da UNOESTE com recidiva do quadro, onde foi realizado novamente o procedimento anterior. Neste caso, foi recomendado alimentação pastosa por toda a vida, devido a sua predisposição ao fecaloma. Palavras-chave: constipação; felino; megacolon.
Keratoconjunctivitis sicca (KCS) is of predominantly immune-mediated origin. Dogs are an excellen... more Keratoconjunctivitis sicca (KCS) is of predominantly immune-mediated origin. Dogs are an excellent model for understanding this disease, as the origin of KCS in dogs is like that in humans. The objective of this study was to localize and quantify immunological markers, such as CD4 lymphocytes, interleukin (IL)-1, IL-6 and tumor necrosis factor alpha (TNFα), before and after topical treatment with mesenchymal stem cells (MSCs). Twentytwo dogs positive for KCS were topically treated with 50 μL (1 × 10 6 MSCs) in the conjunctival sac and were evaluated for 6 months. The levels of the markers CD4, IL-6, IL-1 and TNFα were analyzed in conjunctival biopsy and cytology of the third eyelid gland by immunohistochemistry and immunocytochemistry. The results showed that before treatment, there was marked expression of all the markers (CD4, IL-6, IL-1 and TNFα), and after 6 months, there were significant (p < .05) reductions in the expression levels of all the markers. These results demonstrated that topical MSC treatment promotes a significant decrease in the expression levels of these inflammatory markers and could be used as adjuvant therapy in the treatment of KCS in dogs and humans. In addition, these markers can be excellent tools for diagnosing and analyzing the progression of KCS.
Resveratrol has antioxidant, anti-inflammatory, lipolytic, and antifibrotic properties, which may... more Resveratrol has antioxidant, anti-inflammatory, lipolytic, and antifibrotic properties, which may be useful in supplementation of obese patients and with liver problems. This study evaluated the effects of 6-week resveratrol supplementation on the lipid profile and liver function of female Wistar rats fed a high-fat diet to induce obesity. Sixty-four Wistar rats were divided into 4 groups (n = 16): the control group (C); the control obese group (CO); the resveratrol group (R); and the resveratrol obese group (RO). At the end of the experiment, the animals were anesthetized for blood collection and subsequent euthanasia for collection of liver biopsy. The parameters for body weight, liver weight, retroperitoneal fat weight, serum lipid and liver profiles and histopathological analysis were evaluated. The 6-week resveratrol administration did not induce weight loss nor did it reduce the lipid profile; however, it decreased the liver enzymes aspartate aminotransferase (AST) and alkalin...
Brachycephalic dogs have a normal lower jaw and a receded upper jaw, and these dogs are predispos... more Brachycephalic dogs have a normal lower jaw and a receded upper jaw, and these dogs are predisposed to nostril stenosis, elongated palate, tracheal hypoplasia and hyperthermia due to heat stress. Given these characteristics, these dogs are more predisposed to respiratory and cardiovascular disorders. The popularity of brachycephalic dogs that have a greater tendency for cardiorespiratory disorders, such as French bulldogs, has increased recently; however, studies on the cardiorespiratory and haematological parameters in this breed are lacking. Thus, the present study sought to evaluate the cardiorespiratory and haematological parameters of healthy French bulldogs to standardize normal values for this breed. Thirtysix French bulldogs were used without predilection for age or sex, and the following parameters were evaluated: temperature (T), heart rate (HR), respiratory rate (RR), erythrogram, leukogram, total plasma protein, plasmatic fibrinogen, blood platelet count, urea, creatinine, alkaline phosphatase (AP) and alanine aminotransferase (ALT). The mean and standard deviation were as follows: T°C (38.3±0.4), HR (111.1±27.0) beats per minute, RR (54.8±32.5) breaths per minute, erythrocyte count (7.3±1.2x10 6), haemoglobin (16.9±2.8 g/dL), haematocrit (48.9±7.7%), MCV (67.6±2.4 fL), MCH (23.3±0.7 pg), MCHC (34.6±0.8%), RDW-CV (9.3±0.9; 9.2-12.0%), total leukocytes (11009.7±2907.3/mm 3), band neutrophils (250.0±260.4/mm 3), segmented neutrophils (7094.4±2307.3/mm 3), eosinophils (589.7±561.3/mm 3), lymphocytes (2020.8±1457.5/mm 3), monocytes (987.6±528.4/mm 3), total plasma protein (6.9±0.5 g/dL), plasmatic fibrinogen (274.1±96.5 mg/dL), blood platelet count (366.4±88.5/mm 3), urea (35.2±12.6 mg/dL), creatinine (0.8±0.2/mg/dL), AP (49.1±25.8U/L), ALT (36.2±12.3U/L). The haematological parameters and heart rate were within the normal values for the species, but the respiratory rate values (54.8±32.5 breaths per minute) were greater than the normal average of 10 to 30 breaths per minute. We conclude that the haematological parameters and heart rate of healthy French bulldogs are within the normal limit for dogs; however, the respiratory rate is above reference values, reinforcing the notion that dogs of this breed, although healthy, exhibit an increased risk of respiratory disorders.
Background and Aim: Keratoconjunctivitis sicca (KCS) is predominantly an immune-mediated chronic ... more Background and Aim: Keratoconjunctivitis sicca (KCS) is predominantly an immune-mediated chronic inflammatory ocular disease that is commonly diagnosed in dogs. This study aimed to compare the conventional use of topical immunosuppressant tacrolimus 0.03% eye drops and a new therapy injectable homologous platelet-rich plasma (HPRP) into the third eyelid gland and inferior and superior palpebral conjunctiva of dogs with KCS. Materials and Methods: A total of 66 eyes from 33 dogs were evaluated. The eyes were divided into three equal groups: Negative control group, tacrolimus group (TG), and homologous platelet-rich plasma group (HPRPG). The animals were evaluated using the Schirmer's tear test-1 (STT-1), osmolarity test (OT), strip meniscometry test (SMT), tear film break-up test (TBUT), fluorescein test, lissamine green test (LGT), and cytological and histopathological analyses. Results: In TG, there was a significant increase (p < 0.05) in the STT-1 and SMT values, and goble...
Background and Aim: Tonometers are an important instrument for measuring intraocular pressure (IO... more Background and Aim: Tonometers are an important instrument for measuring intraocular pressure (IOP) in the diagnosis of glaucoma or uveitis. This study aimed to compare the accuracy of the main types of tonometers with different IOP measurement methodologies in dogs: TonoVet and TonoVet Plus (rebound), Tono-Pen Avia Vet (applanation), and Kowa HA-2 (Goldmann applanation). Materials and Methods: IOP was measured in 152 eyes of 76 dogs. A postmortem study was performed by comparing manometry and tonometry values and calculating the correlation coefficient (r2), in vivo real IOP (manometry) among the tonometers was compared, and an outpatient study was conducted with healthy eyes and eyes with signs of glaucoma and uveitis. Results: In the postmortem study, the values of r2 in descending order were Kowa (0.989), TonoVet Plus (0.984), TonoVet (0.981), and Tono-Pen Avia Vet (0.847). The IOP values in mmHg in the in vivo study were as follows: Aneroid manometer (16.8±2.5.7), TonoVet (18.1...
The objective of this study was to compare the efficacy of two sources of omega 3 and 6, fish oil... more The objective of this study was to compare the efficacy of two sources of omega 3 and 6, fish oil (FO) and linseed oil (LO), orally administered, alone or in combination, for treating experimentally induced keratoconjunctivitis sicca (KCS) in rabbits. Twenty-eight New Zealand rabbits were used in this study. Seven animals were allocated to the C group (negative control), and KCS was induced in 21 animals by topically applying 1% atropine sulfate drops for 7 days. Treatment with atropine was maintained throughout the study period (12 weeks). The rabbits were divided into 3 treatment groups containing 7 animals each: FO group, LO group and FLO group (FO and LO). The animals were evaluated using the Schirmer Tear Test I (STT I), Rose Bengal Test (RBT), fluorescein test (FT), tear film break-up time (TBUT), and conjunctival and histopathological analysis. There was a significant increase in STT I and TBUT values in treatment groups, but the increase occurred earlier in the FO group. The...
To evaluate the effectiveness of topical 1% cyclosporine eye drops diluted in either of the two v... more To evaluate the effectiveness of topical 1% cyclosporine eye drops diluted in either of the two vehicles-olive and linseed oil-and that of the oils themselves in treating experimentally-induced keratoconjunctivitis sicca (KCS) in rabbits. KCS was induced in 25 New Zealand rabbits using 1% atropine sulfate eye drops for 7 days before treatment and throughout the treatment period (12 weeks). The rabbits were divided into five groups: one control (C) group without KCS induction and four treatment groups in which KCS was induced and treated topically with olive oil (O), linseed oil (L), cyclosporine in olive oil (CO), and cyclosporine in linseed oil (CL). The animals were evaluated using Schirmer tear test 1 (STT), the fluorescein test (FT), tear-film break-up time (TBUT), the rose bengal test (RBT), and histopathological analysis. Values of STT and TBUT significantly decreased 1 week post-induction (p<0.05) and were similar to initial values after the 4th week of treatment, in all g...
Animais do HV da UNOESTE. 3 Residente do Laboratório Clínico do HV da UNOESTE RESUMO A anemia hem... more Animais do HV da UNOESTE. 3 Residente do Laboratório Clínico do HV da UNOESTE RESUMO A anemia hemolítica imunomediada (AHIM) é definida como uma redução do número de eritrócitos em decorrência da destruição dos mesmos pelo sistema imune devido à presença de imunoglobulinas (IgM e IgG) na membrana do eritrócito. Foram atendidos dois casos desta enfermidade, os quais foram diagnosticados pela somatória historia clínica, resultados dos exames e evolução positiva mediante tratamento clínico efetivo. Esse relato tem por objetivo fazer uma breve revisão do assunto e contribuir com mais dados sobre essa doença. Palavras-chave: Anemia imunomediada. Esferócitos. Hemólise. Cães.
Journal of Ophthalmic Inflammation and Infection, 2013
Background The objective of this study was to evaluate the effectiveness of various linseed oil (... more Background The objective of this study was to evaluate the effectiveness of various linseed oil (LO) preparations (oral, topical, oral and topical combined) in treating experimentally induced keratoconjunctivitis sicca (KCS) in rabbits. Twenty male New Zealand white rabbits were divided into four groups: group C (control), group OLO (oral LO), group TLO (topical LO), and group OTLO (oral and topical LO). The animals were evaluated weekly using Schirmer's tear test (STT), fluorescein test (FT), and Rose Bengal test (RBT) and were euthanized at the end of the experiment for histopathological analysis. Findings There were significant improvements in the parameters analyzed (STT, FT, and RBT) and in the histopathological finding in all of the groups using LO. Conclusions The analyzed results demonstrate that LO, administered orally or topically, was effective in treating experimentally induced KCS in rabbits, although combined oral and topical LO did not show additional benefits gre...
To compare the efficacy of 0.03% tacrolimus eye drops diluted in two different vehicles (linseed ... more To compare the efficacy of 0.03% tacrolimus eye drops diluted in two different vehicles (linseed oil and olive oil) for the treatment of keratoconjunctivitis sicca (KCS) in dogs. This study included 60 dogs. Of this group, 20 were healthy and allocated to the control group, and 40 were diagnosed with bilateral KCS and randomly allocated to either the TO (tacrolimus in olive oil) or the TL (tacrolimus in linseed oil) groups. Ophthalmic examinations, Schirmer Tear Test-1 (STT-1), Tear Film Break-up Time (TBUT) and Fluorescein Test (FT) were carried out monthly, along with cytological and histopathological examinations at the beginning and end of the study. The clinical signs, corneal ulcers, Schirmer Tear Test-1 values, and Tear Film Break-up Time values improved in both groups after one month of treatment. Cytological examination at the end of the study showed decreased lymphocytes, neutrophil, metaplastic, and squamous cell counts in both groups, while the histopathological analysis...
Background and Aim: Keratoconjunctivitis sicca (KCS) is predominantly an immune-mediated chronic ... more Background and Aim: Keratoconjunctivitis sicca (KCS) is predominantly an immune-mediated chronic inflammatory ocular disease that is commonly diagnosed in dogs. This study aimed to compare the conventional use of topical immunosuppressant tacrolimus 0.03% eye drops and a new therapy injectable homologous platelet-rich plasma (HPRP) into the third eyelid gland and inferior and superior palpebral conjunctiva of dogs with KCS.
Materials and Methods: A total of 66 eyes from 33 dogs were evaluated. The eyes were divided into three equal groups: Negative control group, tacrolimus group (TG), and homologous platelet-rich plasma group (HPRPG). The animals were evaluated using the Schirmer's tear test-1 (STT-1), osmolarity test (OT), strip meniscometry test (SMT), tear film break-up test (TBUT), fluorescein test, lissamine green test (LGT), and cytological and histopathological analyses.
Results: In TG, there was a significant increase (p < 0.05) in the STT-1 and SMT values, and goblet cell count in the palpebral conjunctiva by the end of the study. In HPRPG, 36% (four dogs) received three applications, 55% (six dogs) received two applications, and 9% (one dog) received one application before the initial ocular signs improved. There was a significant decrease (p < 0.05) in the lymphocyte and neutrophil counts of the palpebral conjunctiva in HPRPG than in TG. Both groups showed equivalent improvements in TBUT, OT, and LGT values.
Conclusion: Tacrolimus 0.03% eye drops were more efficient than HPRP in increasing tear production and the number of goblet cells. However, injectable HPRP was more efficient than tacrolimus in decreasing the number of conjunctival inflammatory cells. Treatment with injectable HPRP requires an average of two to three applications, is safe and feasible, and can be used as a cheaper alternative or as an adjuvant to conventional treatment with topical immunosuppressants.
Brazilian Journal of Veterinary Research and Animal Science, 2008
O objetivo do presente trabalho foi estudar as características em comum e as diferenças observada... more O objetivo do presente trabalho foi estudar as características em comum e as diferenças observadas na intoxicação induzida por 1 mg/kg de amitraz, IV, em cães e gatos. Os principais sinais clínicos observados em comum foram sedação, hipotermia, bradicardia, bradiarritmias, hipotensão, bradipnéia, midríase e hiperglicemia transitória, porém a intensidade destes sinais foi diferente entre as espécies. A hipotermia foi mais acentuada em gatos. Os cães foram mais sensíveis às alterações cardiorespiratórias apresentando diminuição mais significativa na freqüência cardíaca e respiratória, além de ocorrência de maior número de bradiarritmias. Os gatos apresentaram midríase mais prolongada do que os cães. Observou-se hiperglicemia e hipoinsulinemia transitórias e diminuição transitória dos níveis plasmáticos de cortisol em ambas espécies, porém os gatos apresentaram um pico de hiperglicemia maior e mais precoce do que os cães, e com relação aos níveis plasmáticos de cortisol, os cães aprese...
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Papers by Silvia Andrade
Materials and Methods: A total of 66 eyes from 33 dogs were evaluated. The eyes were divided into three equal groups: Negative control group, tacrolimus group (TG), and homologous platelet-rich plasma group (HPRPG). The animals were evaluated using the Schirmer's tear test-1 (STT-1), osmolarity test (OT), strip meniscometry test (SMT), tear film break-up test (TBUT), fluorescein test, lissamine green test (LGT), and cytological and histopathological analyses.
Results: In TG, there was a significant increase (p < 0.05) in the STT-1 and SMT values, and goblet cell count in the palpebral conjunctiva by the end of the study. In HPRPG, 36% (four dogs) received three applications, 55% (six dogs) received two applications, and 9% (one dog) received one application before the initial ocular signs improved. There was a significant decrease (p < 0.05) in the lymphocyte and neutrophil counts of the palpebral conjunctiva in HPRPG than in TG. Both groups showed equivalent improvements in TBUT, OT, and LGT values.
Conclusion: Tacrolimus 0.03% eye drops were more efficient than HPRP in increasing tear production and the number of goblet cells. However, injectable HPRP was more efficient than tacrolimus in decreasing the number of conjunctival inflammatory cells. Treatment with injectable HPRP requires an average of two to three applications, is safe and feasible, and can be used as a cheaper alternative or as an adjuvant to conventional treatment with topical immunosuppressants.
Materials and Methods: A total of 66 eyes from 33 dogs were evaluated. The eyes were divided into three equal groups: Negative control group, tacrolimus group (TG), and homologous platelet-rich plasma group (HPRPG). The animals were evaluated using the Schirmer's tear test-1 (STT-1), osmolarity test (OT), strip meniscometry test (SMT), tear film break-up test (TBUT), fluorescein test, lissamine green test (LGT), and cytological and histopathological analyses.
Results: In TG, there was a significant increase (p < 0.05) in the STT-1 and SMT values, and goblet cell count in the palpebral conjunctiva by the end of the study. In HPRPG, 36% (four dogs) received three applications, 55% (six dogs) received two applications, and 9% (one dog) received one application before the initial ocular signs improved. There was a significant decrease (p < 0.05) in the lymphocyte and neutrophil counts of the palpebral conjunctiva in HPRPG than in TG. Both groups showed equivalent improvements in TBUT, OT, and LGT values.
Conclusion: Tacrolimus 0.03% eye drops were more efficient than HPRP in increasing tear production and the number of goblet cells. However, injectable HPRP was more efficient than tacrolimus in decreasing the number of conjunctival inflammatory cells. Treatment with injectable HPRP requires an average of two to three applications, is safe and feasible, and can be used as a cheaper alternative or as an adjuvant to conventional treatment with topical immunosuppressants.