Papers by Sharukh Khajotia
Journal - Oklahoma Dental Association
Bottled water use and lack of adequate labeling have given rise to questions regarding fluoride i... more Bottled water use and lack of adequate labeling have given rise to questions regarding fluoride intake. This study examined fluoride concentration of commercially available bottled water products, determined if significant differences existed among the products, and explored packaging date as a variable on the concentration of fluoride. The values were compared to the ADA guideline for optimally fluoridated water and the American Academy of Pediatric Dentistry recommended schedule for fluoride supplementation. Five commercial brands were chosen for the study. Three bottles of each brand, each with different batch dates, were purchased. Following calibration, six tests were conducted on each bottle using the Hach DR-100 Colorimeter System for fluoride analysis and the values were recorded. Data were analyzed using a two-tailed ANOVA and Tukey's HSD Test (p = 0.05). The results showed that significant differences in fluoride concentrations existed among the five brands. For three brands, significant differences existed between the batches. All products had fluoride concentrations that fell below the ADA accepted standards for optimally fluoridated water. Due to the significant variability in fluoride concentration between products and batches, dental providers should advise their patients that bottled water might not contain optimal levels of fluoride.
Quintessence international
ABSTRACT
Objective: To determine the effect of addition of a novel quaternary ammonium, methacrylate-funct... more Objective: To determine the effect of addition of a novel quaternary ammonium, methacrylate-functionalized silicate (QAMS) antimicrobial agent on selected physical and mechanical properties of a commercial light-cured dental resin cement. Method: A sol/gel process was used to fabricate a molecule based on a 1:1:3 molar ratio of tetraethoxysilane (TEOS), AEM5772 (a quaternary ammonium silane), and 3-methacryloxypropyltrimethoxysilane (3-MPTS), by mixing with water (pH 1) to cause 50% hydrolysis. The reaction product was incorporated into triethyleneglycol dimethacrylate, where further compounding resulted in a final QAMS concentration of 3 wt% in a commercially available, light-cured dental resin cement (CHOICE2, BISCO). Disc-shaped specimens of the material with and without QAMS (control) content were light-cured under similar conditions, and subjected to a variety of mechanical and physical property testing: monomer conversion and volumetric polymerization shrinkage 10-minutes foll...
Quintessence international (Berlin, Germany : 1985), 2002
The aim of this study was to assess the effect on microleakage of cyclic lateral fatigue forces i... more The aim of this study was to assess the effect on microleakage of cyclic lateral fatigue forces in different types of cervical resin composite restorations. V-notch cavities were prepared at the cementoenamel junction in 60 extracted human premolars and restored with a bonding system along with one of the following resin composites: hybrid resin composite, microfilled resin composite, and flowable resin composite (20 each). Ten specimens from each group of 20 were subjected to 8,400 cycles of lateral fatigue forces (44 N) at the occlusal half of the clinical crown in a machine specifically developed for this purpose. The other 10 specimens of each group served as controls, representing the microleakage that occurred without lateral fatigue forces. All specimens were stained with a 50% silver nitrate solution and sectioned sagittally. A scoring system of 0 to 6 was used to record the amount of die penetration along the restoration-tooth interface. There was leakage in both the fatigu...
The aim of this work was to evaluate the anti-bacterial properties of chitosan and chitosan/polyc... more The aim of this work was to evaluate the anti-bacterial properties of chitosan and chitosan/polycaprolactone blend membranes against oral pathogens Streptococcus mutans and Actinobacillus actinomycetemcomitans. Membranes were suspended in bacterial cultures and incubated for 24hours. The bactericidal effect (by optical density measurements and agar plating) and bacterial adhesion (by digital imaging and scanning electron microscopy) of the membranes were studied.
ABSTRACT Atomic force microscopy has been used previously to study the morphology of Streptococcu... more ABSTRACT Atomic force microscopy has been used previously to study the morphology of Streptococcus mutans bacteria and dehydrated biofilms. Objectives: To characterize the topographies of hydrated S. mutans biofilms during the first three hours of formation on resin-composite. Methods: Specimens of Point 4 microhybrid resin-composite (Kerr Corp.; d=6.0mm, h=0.3mm) were sequentially wet-polished (MultiPrep grinder-polisher, Allied High Tech; 180 through 1200grit SiC disks), sterilized (ethylene oxide) and divided into two groups (n=9/group). Streptococcus mutans (UA159) biofilms were grown on specimens in 0.5x Tryptone-Yeast extract medium supplemented with 0.17% sucrose for 1, 2, and 3 hours, then washed with sterile, ultrapure water to constitute the Biofilm group. Control group specimens were subjected to the same growth protocol but without bacterial innoculum. Specimens were scanned at 37C in PBS using a MultiMode-AFM/NanoScope-V controller (tapping-mode; 100x100m scans @0.25Hz; NP-S probes). Scans were flattened (NanoScope software) and 21 topographical parameters were calculated. Parameters were compared using two-factor ANOVA and post hoc SNK tests (α=0.05). Results: Statistically significant differences were observed between biofilm and control groups (p<0.02) and among the time intervals tested (p<0.02) for parameters root mean square roughness (Rq), ten-point mean roughness (Rz) and average maximum height (Rpm). Time Rq(nm) Rz(nm) Rpm(nm) Biofilm Control Biofilm Control Biofilm Control 1h 75.0362.71A 39.806.58A 48.2322.92A 43.936.20A 18.608.21A 20.233.93A 2h 103.135.39A 42.033.61A 82.073.42A 43.375.83A 36.832.45A 19.502.75A 3h 288.00114.41B 41.307.89A 268.67149.02B 47.108.34A 147.9079.63B 21.003.86A *Identical superscript letters denote mean values that are not statistically different within each column (p>0.05) Conclusions: AFM scans of S. mutans biofilms demonstrated bacterial and EPS components via roughness profiles more clearly than comparable confocal microscopy scans. Hydrated biofilms grown on resin-composite had more complex morphologies after 3 hours of growth than shorter durations. These results suggest that AFM could be adapted for the noninvasive study of the nanoscale topography of actively growing biofilms on dental surfaces. Funding: NIH grants P20RR018741 and R15DE019566.
Atomic force microscopy has been used previously to study the morphology of Streptococcus mutans ... more Atomic force microscopy has been used previously to study the morphology of Streptococcus mutans bacteria and dehydrated biofilms. Objectives: To characterize the topographies of hydrated S. mutans biofilms during the first three hours of formation on resin-composite. Methods: Specimens of Point 4 microhybrid resin-composite (Kerr Corp.; d=6.0mm, h=0.3mm) were sequentially wet-polished (MultiPrep grinder-polisher, Allied High Tech; 180 through 1200grit SiC disks), sterilized (ethylene oxide) and divided into two groups (n=9/group). Streptococcus mutans (UA159) biofilms were grown on specimens in 0.5x Tryptone-Yeast extract medium supplemented with 0.17% sucrose for 1, 2, and 3 hours, then washed with sterile, ultrapure water to constitute the Biofilm group. Control group specimens were subjected to the same growth protocol but without bacterial innoculum. Specimens were scanned at 37C in PBS using a MultiMode-AFM/NanoScope-V controller (tapping-mode; 100x100m scans @0.25Hz; NP-S pro...
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Papers by Sharukh Khajotia