Papers by Seyed A. Mesbah-Namin
PLOS ONE, Nov 16, 2017
The use of mesenchymal stem cells (MSCs) for cell therapy and regenerative medicine has received ... more The use of mesenchymal stem cells (MSCs) for cell therapy and regenerative medicine has received widespread attention over the past few years, but their application can be complicated by factors such as reduction in proliferation potential, the senescent tendency of the MSCs upon expansion and their age-dependent decline in number and function. It was shown that all the mentioned features were accompanied by a reduction in telomerase activity and telomere shortening. Furthermore, the role of epigenetic changes in aging, especially changes in promoter methylation, was reported. In this study, MSCs were isolated from the adipose tissue with enzymatic digestion. In addition, immunocytochemistry staining and flow cytometric analysis were performed to investigate the cell-surface markers. In addition, alizarin redS , sudan III, toluidine blue, and cresyl violet staining were performed to evaluate the multi-lineage differentiation of hADSCs. In order to improve the effective application of MSCs, these cells were treated with 1.5 × 10 −8 and 2.99 × 10 −10 M of ZnSO 4 for 48 hours. The length of the absolute telomere, human telomerase reverse transcriptase (hTERT) gene expression, telomerase activity, the investigation of methylation status of the hTERT gene promoter and the percentage of senescent cells were analyzed with quantitative realtime PCR, PCR-ELISA TRAP assay, methylation specific PCR (MSP), and beta-galactosidase (SA-β-gal) staining, respectively. The results showed that the telomere length, the hTERT gene expression, and the telomerase activity had significantly increased. In addition, the percentage of senescent cells had significantly decreased and changes in the methylation status of the CpG islands in the hTERT promoter region under treatment with ZnSO 4 were seen. In conclusion, it seems that ZnSO 4 as a proper antioxidant could improve the aging-related features due to lengthening of the telomeres, increasing the telomerase gene
Biomedical Engineering Online, Nov 23, 2017
Background Despite the advances made in scientific research and medical treatment of cardiovascul... more Background Despite the advances made in scientific research and medical treatment of cardiovascular diseases, there are still patients who die from these diseases every year. Regenerative medicine with the use of autologous or allogeneic stem cells isolated from different tissues [1] is trying to repair damaged heart. Stem cells isolated from adipose tissue [2] with high number of pluripotent stromal cells are similar to bone marrow mesenchymal stem cells (BMSCs) in characteristics, surface proteins and differentiation potential [3, 4]. These cells exert their functions via their paracrine effect which improves angiogenesis, reduce ischemic-induced apoptosis, modulate inflammation and enhance progenitor cell recruitment and differentiation [5]. There is a lot of evidence regarding the ability of ADSCs to differentiate into different types of cells [6-8]. Culture on scaffolds is recently used for cell culture and stem cell differentiation. The morphology of the cultured cells in the scaffold is more similar to in vivo condition and
Mycoses, Aug 1, 2002
in 5 (9,3%), including 3 patients were found out antigens to both fungi. Presence in blood of ant... more in 5 (9,3%), including 3 patients were found out antigens to both fungi. Presence in blood of antigens C. albicans and A.fumigatus in all cases was combined with niycoses 45, (Suppl. 2) 3-72 (2002)
British journal of medicine and medical research, Dec 21, 2013
Recently, biomarkers in medicine have gained comprehensive scientific and clinical interest. Biom... more Recently, biomarkers in medicine have gained comprehensive scientific and clinical interest. Biomarker or biological marker defined as alteration in the constituents of tissues or body fluids provide a powerful approach to understanding the spectrum of chronic diseases with application in at least 5 areas like screening, diagnosis, prognostication, prediction of disease recurrence and therapeutic monitoring. Therefore, biomarkers are biological indicators of diseases that can be measured either in vivo by biomedical imaging or in vitro by laboratory methods. Many kinds of biomarkers are available in the field of medical science with lots of positive as well as negative effect. These markers can also reflect the entire spectrum of disease from the earliest manifestations to the terminal stages and will become one of the major driving forces of pharmaceutical research and drug development in the coming years. Generally, a biomarker is potentially useful along the whole spectrum of the disease processbefore diagnosis; for screening and risk assessment, during diagnosis; for staging, grading and selecting the initial therapy and during treatment for monitoring therapy, selecting additional therapy or monitoring recurrent diseases. This brief review describes the types and major uses of biomarkers in clinical investigation. Review Article British Journal of Medicine & Medical Research, 4(8): 1701-1718, 2014 1702
Multiple sclerosis and related disorders, 2016
Regulatory T cells (Treg cells), defined as CD4(+) CD25(+) FoxP3(+) T cells by expression of CD4,... more Regulatory T cells (Treg cells), defined as CD4(+) CD25(+) FoxP3(+) T cells by expression of CD4, high-affinity IL-2 receptor and the transcription factor, forkhead box P3 (FoxP3). They play a pivotal role in protecting individuals from autoimmunity and a growing body of evidence suggests their role in the prevention of multiple sclerosis development. However, there are discrepancies about the type of defect in the Treg cells of multiple sclerosis patients and especially whether the Treg number alteration could be contributed to multiple sclerosis pathogenesis. Indeed, whether low number of Treg cells can be a risk factor contributing to multiple sclerosis pathogenesis is the matter of debate and there is not any comprehensive agreement on it. Thus, the objective of this systematic review and meta-analysis was to precisely quantify the nature and magnitude of the association between Treg cell number and the risk ratio/odds ratio (OR) of multiple sclerosis in the case-control studies. Hence, medical databases of Embase, PubMed/Medline, PubMed, PubMed Central and SCOPUS were searched for empirical papers using "Regulatory T cell frequency", "Treg frequency" in combination with "multiple sclerosis". In the case-control studies, papers were reviewed for inclusion/exclusion criteria and 8 publications were included. Under random-effect model meta-analysis the data showed that the frequency of Treg cells was not a risk factor in multiple sclerosis using current laboratory methods.
DARU, May 28, 2014
Background: Artemisinin is the major sesquiterpene lactones in sweet wormwood (Artemisia annua L.... more Background: Artemisinin is the major sesquiterpene lactones in sweet wormwood (Artemisia annua L.), and its combination with transferrin exhibits versatile anti-cancer activities. Their non-selective targeting for cancer cells, however, limits their application. The aim of this study was to prepare the artemisinin and transferrin-loaded magnetic nanoliposomes in thermosensitive and non-thermosensitive forms and evaluate their antiproliferative activity against MCF-7 and MDA-MB-231 cells for better tumor-targeted therapy. Methods: Artemisinin and transferrin-loaded magnetic nanoliposomes was prepared by extrusion method using various concentrations of lipids. These formulations were characterized for particle size, zeta potential, polydispersity index and shape morphology. The artemisinin and transferrin-loading efficiencies were determined using HPLC. The content of magnetic iron oxide in the nanoliposomes was analysed by spectrophotometry. The in vitro release of artemisinin, transferrin and magnetic iron oxide from vesicles was assessed by keeping of the nanoliposomes at 37°C for 12 h. The in vitro cytotoxicity of prepared nanoliposomes was investigated against MCF-7 and MDA-MB-231 cells using MTT assay. Results: The entrapment efficiencies of artemisinin, transferrin and magnetic iron oxide in the non-thermosensitive nanoliposomes were 89.11% ± 0.23, 85.09% ± 0.31 and 78.10% ± 0.24, respectively. Moreover, the thermosensitive formulation showed a suitable condition for thermal drug release at 42°C and exhibited high antiproliferative activity against MCF-7 and MDA-MB-231 cells in the presence of a magnetic field. Conclusions: Our results showed that the thermosensitive artemisinin and transferrin-loaded magnetic nanoliposomes would be an effective choice for tumor-targeted therapy, due to its suitable stability and high effectiveness.
Biological Trace Element Research, Oct 12, 2022
Regenerative Therapy, Jun 1, 2023
Pathology & Oncology Research, Dec 3, 2018
The aim of this study was to investigate the relationship between p16 methylation and its express... more The aim of this study was to investigate the relationship between p16 methylation and its expression in oral squamous cell carcinoma (OSCC). Also the contribution of clinicopathological factors, HPV infection and smoking in p16 expression and promoter methylation has been investigated. In this study 67 consecutive OSCC patients and 59 normal individuals were enrolled. All patients were candidates for surgery of oral cavity and fresh tumor biopsies were collected and processed for DNA and RNA extraction. Normal gingival tissues were collected from individuals referred to dentistry clinic and considered as controls. All the cases and controls were checked for HPV infection and then promoter methylation and expression of p16 gene were determined using Methylation-specific PCR (MSP) and real-time PCR (QPCR), respectively. Methylation of p16 in tumors and normal tissues were 59.7 and 38.9%, respectively. Most of hypermethylated samples (>82%) were in high grades. P16 methylation was comparable in HPV+ and HPV-patients or smokers. P16 was overexpressed (~3 fold; p = 0.044) in HPV+ tumors, but it was significantly down-regulated in smoker patients (40% of all tumors). Comparison of P16 expression in OSCC tumors with different degrees of promoter methylation further suggest the relationship of methylation rate and downregulation of P16 expression. The p16 methylation and expression was differentially affected in patients with HPV infection and the smoker cases. Regardless of the influence of environmental factors, it appears that P16 status is useful for classifying patients with OSCC and for influencing treatment strategies in accordance with this classification. Moreover, targeting the upregulation of p16 could be a promising therapeutic option.
Journal of the Neurological Sciences, Apr 1, 2017
Multiple sclerosis (MS) is a chronic autoimmune disease with demyelination and neurodegeneration ... more Multiple sclerosis (MS) is a chronic autoimmune disease with demyelination and neurodegeneration of the central nervous system. It has been shown that the regulatory T (Treg) cells are responsible for maintaining tolerance to self-antigens and can suppress the autoimmune process in several autoimmune models such as experimental autoimmune encephalomyelitis (EAE), a mouse model of MS. Recent basic studies have demonstrated that forkhead box P (FOXP3) and BTB domain and CNC homolog 2 (BACH2) are the master transcription factors of these cells playing a pivotal role in the polarization of naïve T cells into Treg cells. In the current study, the expression of FOXP3 and BACH2 genes and FOXP3 promoter methylation were evaluated in T cells of the EAE-induced mice. The results of this study showed a prominent and significant hypermethylation of the FOXP3 gene promoter in the EAE-induced mice compared to the sham and control groups. The expression of FOXP3 and BACH2 genes was significantly decreased in the EAE group in comparison with the sham and control groups. This study suggests that the epigenetic modification of FOXP3 gene is involved in the pathogenesis of EAE and this could be important in therapy in an appropriate and logical statement.
PubMed, Oct 1, 2022
Objectives: Hematopoietic stem cells (HSCs) are the cells that give rise to different types of bl... more Objectives: Hematopoietic stem cells (HSCs) are the cells that give rise to different types of blood cells during the hematopoiesis process. Mesenchymal stromal cells (MSCs) as key elements in the bone marrow (BM) niche interact with hematopoietic progenitor cells (HPCs) by secreting cytokines, which control HPCs maintenance and fate. Here we report that BM-MSCs are capable of inducing granulocytic differentiation of the C-Kit+ HSCs via activating JAK3/STAT3, ERK, and PI3K signaling pathways. Materials and methods: For this purpose, BM-MSCs and C-kit+ HSCs were isolated. Next, cells were divided into two groups and differentiated into granulocytes: C-kit+ HSCs alone (control group) and co-cultured C-kit+ HSCs with MSCs (experimental group). Afterward, the gene and protein expression were assessed by real-time PCR and western blotting, respectively. Results: It was found that BM-MSCs resulted in increased JAK3/STAT3, ERK, and PI3K protein expression in granulocyte differentiated C-kit+ HSCs. Conclusion: It should be concluded that MSCs could affect the granulocyte differentiation of C-kit+ HSCs via increasing JAK3/STAT3, ERK, and PI3K signaling pathways.
Journal of Cancer Research and Clinical Oncology, Jan 19, 2023
Background: MicroRNA (miRNA) is a regulatory molecule capable of positively or negatively regulat... more Background: MicroRNA (miRNA) is a regulatory molecule capable of positively or negatively regulating signaling pathways and furthermore assumes a part tumorigenesis and various aspects of cancer. The purpose of this study is to investigate the expression level of miR-133a, miR-637 and miR-944 genes in serum and tumor tissue and their relationship with the expression level of phosphatidylinositol-3-kinase (PI3K (and protein kinase-B (AKT) genes and proteins and its clinical signi cance in breast cancer. Methods: The expression of miR-133a, miR-637, miR-944, PI3K and AKT genes in tumor tissues and tumor margins tissues of 40 patients with breast cancer, as well as the serum levels of miR-133a, miR-637 and miR-944 in these patients and 40 healthy groups were examined by quantitative real-time PCR (qRT-PCR). PI3K and AKT proteins expression in tumor tissue and tumor margins tissues were detected by immunohistochemistry (IHC). Results: The expression levels of miR-133a and miR-637 in the tumor tissue and serum of patients were lower than the tumor margin tissue and serum of the healthy group, respectively. Also, the expression level of miR-944 in the tumor tissue was lower than in the tumor margin tissue, but its expression increased in the serum of cancer patients compared to the healthy group. The expression of miR-637 was correlated with tumor location, tumor size, and Her2 receptors, as well as the expression of miR-944 with tumor location and family history. PI3K and AKT mRNA and protein levels were higher in tumor tissues compared to tumor margin tissue (p<0.05). Conclusion: The results of our study show that miR-637 has a better diagnostic value in breast cancer than miR-133a and miR-944.
Iranian Journal of Allergy, Asthma and Immunology, 2020
Vitamin D plays a variety of physiological functions, such as regulating mineral homeostasis. Mor... more Vitamin D plays a variety of physiological functions, such as regulating mineral homeostasis. More recently, it has emerged as an immunomodulator player, affecting several types of immune cells, such as regulatory T (Treg) cells. It has been reported that vitamin D exerts some mediatory effects through an epigenetic mechanism. In this study, the impacts of calcitriol, the active form of vitamin D, on the methylation of the conserved non-coding sequence 2 (CNS2) region of the forkhead box P3 (FOXP3) gene promoter, were evaluated. Fourteen C57BL/6 mice were recruited in this study and divided into two intervention and control groups. The CD4+ T cells were isolated from mice splenocytes. The expression of FOXP3, IL-10, and transforming growth factor-beta (TGF-β1) genes were relatively quantified by real-time PCR technique, and the DNA methylation percentage of every CpG site in the CNS2 region was measured individually by bisulfite-sequencing PCR. Vitamin D Intervention could significa...
Food & Function, 2019
After consumption of betalain-rich supplements of red beetroot and betacyanins-rich supplements o... more After consumption of betalain-rich supplements of red beetroot and betacyanins-rich supplements of Opuntia stricta, the betanin appears in urine and plasma to improve the lipid profile, blood pressure, homocysteine and glucose levels of the patients.
International Journal of Laboratory Hematology, 2019
Iranian journal of public health, 2015
Malnutrition and dehydration are two most common types of ailments residents of nursing homes (NH... more Malnutrition and dehydration are two most common types of ailments residents of nursing homes (NH) prone to. It is very important to assess these problems because they can predispose the residents to severe illnesses. The aim of this study was to gather information on nutritional status and its associated risk factors in elderly residents of NHs in Tehran, Iran. From 16 NHs in Tehran, 263 residents were randomly selected. Data were collected via questionnaires, including demographic characteristics, past medical history, present health problems and daily routines. The MNA questionnaire was used to gather information regarding their nutritional status. The present study showed that 10.3% of the elderly residents in nursing homes were malnourished. 66.4% of males and 70.8% of females were at risk of malnutrition. Multivariate analysis showed that after adjusting for confounders the following elderly-related factors were the independent risk factors of malnutrition: consuming half or l...
Journal of neuroimmunology, Jan 15, 2014
Autotaxin (ATX) is an enzyme producing lysophosphatidic acid (LPA) from lysophosphatidyl choline ... more Autotaxin (ATX) is an enzyme producing lysophosphatidic acid (LPA) from lysophosphatidyl choline (LPC) and it is up-regulated in inflammatory conditions such as various cancers, arthritis and multiple sclerosis (MS). Numerous studies have shown that the LPA signaling gives rise to angiogenesis, mitosis, cell proliferation and cytokine secretion. On the one hand, an increasing body of evidence suggests that blockade of ATX has anti-inflammatory properties in a variety of diseases. The aim of this study was to measure the enzyme activity of ATX in cerebrospinal fluid (CSF) and serum of patients with MS using an enzymatic photometric method. Twenty definite relapsing remitting MS patients along with 20 patients with other neurological diseases (OND) were recruited. The results showed that ATX activity was significantly higher (p value<0.0001) in MS patients than those patients diagnosed with OND. It is possible that inhibition of the ATX may decrease the rate of MS relapses/progress...
Nutritional Neuroscience, 2014
Journal of Neuroimmunology, 2013
An increasing body of evidence suggests that patients afflicted with multiple sclerosis (MS) show... more An increasing body of evidence suggests that patients afflicted with multiple sclerosis (MS) show alterations in immunologic markers including increases in proinflammatory cytokine activity and inflammation. Secretory Phospholipase A2 (sPLA2) is one of the key molecules contributing to the production of inflammatory lipid mediators, mainly eicosanoids. They are considered proinflammatory enzymes and their inhibition has long been recognized as a desirable therapeutic target. The aim of this study was to measure the enzyme activity of sPLA2 both in serum and cerebrospinal fluid (CSF) of MS patients. Twenty MS patients accompanied with 20 age-sex matched controls were recruited. The results showed that the enzyme activity of serum sPLA2 was 0.007 ± 0.021 (μmol/min/ml) in MS patients vs. 0.007 ± 0.016 (μmol/min/ml) in patients with other neurological diseases as a control group (P = 0.5). Our findings also indicated that there is no correlation (P = 0.6) between CSF sPLA2 enzyme activity and MS disease when the results of two groups were compared (0.072 ± 0.020 in cases vs. 0.071 ± 0.01 in control group). The results suggest that the enzyme activity of sPLA2 is not altered during the disease course.
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Papers by Seyed A. Mesbah-Namin