Most of the mutations described in human MDM2 are tolerated without significantly disrupting the ... more Most of the mutations described in human MDM2 are tolerated without significantly disrupting the corresponding structural or molecular function. However, some of them are associated with a variety of human diseases, including cancer. Numerous computational methods have been developed to predict the effects of missense single nucleotide variants (SNVs). The non-synonymous single nucleotide polymorphisms affect the function of XRCC1, which impairs the ability to repair DNA and therefore increases the risk of diseases such as cancer. In this study, sequence and structure-based computational tools were used to screen the total listed coding SNPs of the MDM2 gene in order to recognize and describe them. The potential 6 ns SNP of MDM2 were identified from 29 ns SNP by consistent analysis using computational tools PolyPhen 2, SIFT, PANTHER and cSNP. The computational methods were used to systematically classify functional mutations in the regulatory and coding regions that modify the expre...
1st International Conference on Applied Chemical, Biological and Agricultural Sciences
The present study was carried out to estimate interrelationship and genetic diversity in M3 gener... more The present study was carried out to estimate interrelationship and genetic diversity in M3 generation of Vigna radiata in response to gamma radiation. The experimental materials comprised the four irradiated lines of mungbean AUM-18, AUM-19, AUM-31 and M-2004 that were irradiated at 250 Gy, 350 Gy and 450 Gy doses of gamma rays. The experiment was laid out in split plot design, with two replications. Each replication was divided into main plot and subplot. Dose levels were assigned to main plots and varieties in subplots. High heritability estimates were observed for all traits. Phenotypic correlation of seed yield per plant with germination percentage and seeds per pod was positive and highly significant however positive and significant phenotypic correlation of clusters per plant and 100-seed weight while pods per plant was negative and significant. Higher heritability and significance correlation indicated that germination percentage, 100-seed weight and seeds per pod may be use...
Mongolian Medicinal Formula Anshen-Buxin-Liuwei Pill Alleviate Cardiomyocyte Hypoxia/Reoxygenation Injury via Mitochondrion Pathway
<jats:title>Abstract</jats:title> <jats:p>Anshen Buxin Liuwei pill (ABLP), a Mo... more <jats:title>Abstract</jats:title> <jats:p>Anshen Buxin Liuwei pill (ABLP), a Mongolian medicinal formula, composed of the six medicinal materials of Mongolian medicine <jats:italic>Bos taurus domesticus</jats:italic> Gmelin, <jats:italic>Choerospondias axillaris</jats:italic> (Roxb. ) Burtt et Hill, <jats:italic>Myristica fragrans</jats:italic> Houtt., <jats:italic>Eugenia caryophµllata</jats:italic> Thunb., <jats:italic>Aucklandia lappa</jats:italic> Decne., <jats:italic>Liqui dambar formosana</jats:italic> Hance, is considered to have a therapeutic effect on the symptoms such as coronary heart disease, angina pectoris, arrhythmia, depression and irritability, palpitation, and shortness of breath. Therefore, the present study was employed a network pharmacology approach to identify the potentially active ingredients and to evaluate the protective effect of ABLP on hypoxia/reoxygenation (HR)-induced H9c2 cardiomyocytes, and its influence on cell viability, apoptosis, oxidative stress. H9c2 cardiomyocytes were used to construct a HR injury model. CCK-8 assay and AnnexinV-FITC cell apoptosis assays were used for cell viability and cell apoptosis determination. The levels of LDH, SOD, MDA, CAT, CK, GSH-Px, Na<jats:sup>+</jats:sup>-K<jats:sup>+</jats:sup>-ATPase, and Ca<jats:sup>2+</jats:sup>-ATPase in the cells were determined to assess the effect of ABLP. the mRNA levels of Sirtuin3 (Sirt3) and Cytochrome C (Cytc) in H9c2 cells were determined by quantitative real-time PCR. The finding of this study indicates that HR treatment cells began to shrink from the spindle in an irregular shape with some floated in the medium, well by increasing the therapeutic dose of ABLP (5 µg/mL, 25 µg/mL, and 50 µg/mL), the cells gradually reconverted in a concentration-dependent manner. The release of CK in HR-treated cells was significantly increased, indicating that ABLP exerts a protective effect in H9c2 cells against HR injury and can improve the mitochondrial energy metabolism and mitochondrial function integrity. The present study scrutinized the cardio-protective effect of ABLP against the HR-induced H9c2 cells injury through antioxidant and mitochondrion pathways. ABLP could be a promising therapeutic drug for the treatment of myocardial ischemic cardiovascular disease. The results will provide reasonable information for clinical use of the ABLP.</jats:p>
Evidence-Based Complementary and Alternative Medicine
Background. Anshen Buxin Liuwei pill (ABLP) is a Mongolian medicinal formula which has a therapeu... more Background. Anshen Buxin Liuwei pill (ABLP) is a Mongolian medicinal formula which has a therapeutic effect on the symptoms such as coronary heart disease, angina pectoris, arrhythmia, depression and irritability, palpitation, and short breath. However, its bioactivity against cardiac injury remains unclear. Methods. The protective effect of ABLP was evaluated using H9c2 cells. Cell viability, intracellular Ca2+, reactive oxidative indices, and mitochondrial membrane potential (∆ψ) were assessed, respectively. The mRNA levels of Ca2+ channel-related genes (DHPR, RyR2, and SCN5A) and oxidative stress-related genes (Keap1, Nrf2, and HO-1) were measured by RT-PCR. Results. 0.5–50 μg/mL ABLP could significantly decrease H2O2-induced cell injury by suppressing cell necrosis/apoptosis and excess oxidative stress, ameliorating the collapse of ∆ψ, and reducing intracellular Ca2+ concentration. Furthermore, 0.5–50 μg/mL ABLP reversed H2O2-induced imbalance in the mRNA levels of DHPR, RyR2, S...
The present study was carried out to estimate inter relationship and genetic diversity in M 3 gen... more The present study was carried out to estimate inter relationship and genetic diversity in M 3 generation of Vigna radiata in response to gamma radiation. The experimental m aterials comprised the four irradiated lines of mungbean AUM-18, AUM-19, AUM-31 and M-2004 that wer e i radiated at 250 Gy, 350 Gy and 450 Gy doses of gamma rays. The experiment was laid out in split pl o design, with two replications. Each replication was divided into main plot and subplot. Dose levels were assign ed to main plots and varieties in subplots. High he ritability estimates were observed for all traits. Phenotypic correlation of seed yield per plant with germinatio n percentage and seeds per pod was positive and highly significant h owever positive and significant phenotypic correlat ion of clusters per plant and 100-seed weight while pods per plant was negative and significant. Higher heritability a nd significance correlation indicated that germination percentage, 100-seed weight and seeds per pod ...
Toll-like receptors (TLRs) are innate immune receptors that mediate the inflammatory response dur... more Toll-like receptors (TLRs) are innate immune receptors that mediate the inflammatory response during HCV infections. The goal of this study was to evaluate the association of TLR9 gene polymorphism (rs5743836) in Pakistani patients infected with genotype 3a of HCV. Total 500 subjects were recruited, 400 HCV patients and 100 healthy individuals. Genotyping of TLR9 (-1237T/C, rs5743836) was carried out in 400 HCV patients (323 interferon responders and 77 interferon non responder) and control group by applying High resolution melting (HRM) curve assay. No remarkable differences in distribution of genotype between HCV (p<0.0001; OR= 3.21, 95% CI= (2.514.12) and control groups (p<0.0001; OR=0.092, 95%CI= (0.0580.14) were observed. In conclusion TLR9-1237T/C gene polymorphism may not be considered as a molecular risk for patients with HCV in Pakistan.
Estimation of genetic association among yield contributing traits in aromatic and non-aromatic rice (Oryza sativa L) cultivars
This study provided useful evidences about yield and its contributing components on 8 morphologic... more This study provided useful evidences about yield and its contributing components on 8 morphological traits, which were studied in 24 Asian accessions of rice (Oryza sativa L.). Plant yield was positively and significantly correlated with filled grains weight per panicle, number of grains per panicle, 1000-grain weight and spikelet fertility percentage at genotypic and phenotypic levels. Thus, these traits could play pivotal role in the development of high yielding rice genotypes. Path coefficient analysis recorded highest positive direct effect on yield per plant through number of grains per panicle, filled grains weight per panicle, plant height and 1000-grain weight. Hence, traits like number of grains per panicle, filled grains weight per panicle and 1000-grain weight would be useful in future breeding programs to develop superior genotypes having excellent yield potential. [Naseer, S, Kashif M, Ahmad HM, Iqbal MS Ali Q. Estimation of genetic association among yield contributing ...
The experiment was conducted to reveal genetic variability and to study the parameter analysis in... more The experiment was conducted to reveal genetic variability and to study the parameter analysis in 24 aromatic and non-aromatic rice genotypes. Highly significant variations were determined and the phenotypic variance was relatively greater than genotypic variance for most of the traits, like plant height, number of spikelets per panicle and number of grains per panicle. Higher magnitude of phenotypic variance showed more effect of environment on expression of these traits. A few traits such as number of tillers per plant, spikelet fertility percentage, filled grains weight per panicle, 1000-grain weight and yield per plant indicated least differences between PV and GV, this phenomenon gave the information of gene control for expressivity of these traits. Highest GCV was observed for number of tillers per plant, then yield per plant, number of grains per panicle and filled grains weight per panicle, while spikelet fertility percentage showed lowest value of GCV. Sufficient amount of ...
Journal of Experimental Biology and Agricultural Sciences
The emergence of a novel coronavirus referred to as SARS-CoV-2 has become a global health apprehe... more The emergence of a novel coronavirus referred to as SARS-CoV-2 has become a global health apprehension due to rapid transmission tendency, severity, and wide geographical spread. This emergence was started from Wuhan, China in 2019 from the zoonotic source and spread worldwide, infecting almost half of the community on this earth. Many of the receptors are involved in proceeding with this infection in the organism's body. Toll-like receptors (TLRs) play essential and protective functions from a wide range of microbial pathogens. Small setup of TLR adaptor proteins leads to activate nuclear factor kappa B (NF-kB) and interferon-regulatory factor (IRF). Consequently, various advanced inflammatory cytokines, chemokines, and interferon reaction properties can be up-regulated. Similarly, TLR flagging works on autophagy in macrophages. Autophagy is a cell response to starvation that helps to eliminate damaged cytosol organelles and persistent proteins. It is also able to prevent the r...
Severe Acute Respiratory Syndrome Coronaviruses and 21ST Century Pandemic: An Overview of Functional Receptors and Challenge of Therapeutic Success
Journal of Experimental Biology and Agricultural Sciences
Sudden prevalence of coronavirus disease-2019 (COVID-19) has badly impeded and collapsed the emer... more Sudden prevalence of coronavirus disease-2019 (COVID-19) has badly impeded and collapsed the emerging global trend of economic development by its ongoing pandemic. The novel coronavirus also named severe acute respiratory syndrome virus – 2 (SARS-CoV-2) causes the disease COVID-19 that influences the health management of humans and the world commerce badly. It has affected the human social lives and education in underdeveloped countries and severely impeded industries, organizations, agriculture, etc. Three perceptible types of SARS-CoV-2 strains have been discovered. Each of them has specific receptors, and some of them are common in SARS and SARS-CoV-2. Among them, the ACE2 receptor is believed to be the central receptor of human infectious coronaviruses. It supports mainly to get access, enter into the cell, and causes the basic infection. Similarly, TMPRSS2 is also acting as a portal for a virus to get an approach to the cell and does not support metabolic processes like replica...
Evidence-Based Complementary and Alternative Medicine
Background. Anshen Buxin Liuwei pill (ABLP) is a Mongolian medicinal formula which has a therapeu... more Background. Anshen Buxin Liuwei pill (ABLP) is a Mongolian medicinal formula which has a therapeutic effect on the symptoms such as coronary heart disease, angina pectoris, arrhythmia, depression and irritability, palpitation, and short breath. However, its bioactivity against cardiac injury remains unclear. Methods. The protective effect of ABLP was evaluated using H9c2 cells. Cell viability, intracellular Ca2+, reactive oxidative indices, and mitochondrial membrane potential (∆ψ) were assessed, respectively. The mRNA levels of Ca2+ channel-related genes (DHPR, RyR2, and SCN5A) and oxidative stress-related genes (Keap1, Nrf2, and HO-1) were measured by RT-PCR. Results. 0.5–50 μg/mL ABLP could significantly decrease H2O2-induced cell injury by suppressing cell necrosis/apoptosis and excess oxidative stress, ameliorating the collapse of ∆ψ, and reducing intracellular Ca2+ concentration. Furthermore, 0.5–50 μg/mL ABLP reversed H2O2-induced imbalance in the mRNA levels of DHPR, RyR2, S...
The present study was carried out to estimate inter relationship and genetic diversity in M 3 gen... more The present study was carried out to estimate inter relationship and genetic diversity in M 3 generation of Vigna radiata in response to gamma radiation. The experimental m aterials comprised the four irradiated lines of mungbean AUM-18, AUM-19, AUM-31 and M-2004 that wer e i radiated at 250 Gy, 350 Gy and 450 Gy doses of gamma rays. The experiment was laid out in split pl o design, with two replications. Each replication was divided into main plot and subplot. Dose levels were assign ed to main plots and varieties in subplots. High he ritability estimates were observed for all traits. Phenotypic correlation of seed yield per plant with germinatio n percentage and seeds per pod was positive and highly significant h owever positive and significant phenotypic correlat ion of clusters per plant and 100-seed weight while pods per plant was negative and significant. Higher heritability a nd significance correlation indicated that germination percentage, 100-seed weight and seeds per pod ...
Toll-like receptors (TLRs) are innate immune receptors that mediate the inflammatory response dur... more Toll-like receptors (TLRs) are innate immune receptors that mediate the inflammatory response during HCV infections. The goal of this study was to evaluate the association of TLR9 gene polymorphism (rs5743836) in Pakistani patients infected with genotype 3a of HCV. Total 500 subjects were recruited, 400 HCV patients and 100 healthy individuals. Genotyping of TLR9 (-1237T/C, rs5743836) was carried out in 400 HCV patients (323 interferon responders and 77 interferon non responder) and control group by applying High resolution melting (HRM) curve assay. No remarkable differences in distribution of genotype between HCV (p<0.0001; OR= 3.21, 95% CI= (2.514.12) and control groups (p<0.0001; OR=0.092, 95%CI= (0.0580.14) were observed. In conclusion TLR9-1237T/C gene polymorphism may not be considered as a molecular risk for patients with HCV in Pakistan.
Estimation of genetic association among yield contributing traits in aromatic and non-aromatic rice (Oryza sativa L) cultivars
This study provided useful evidences about yield and its contributing components on 8 morphologic... more This study provided useful evidences about yield and its contributing components on 8 morphological traits, which were studied in 24 Asian accessions of rice (Oryza sativa L.). Plant yield was positively and significantly correlated with filled grains weight per panicle, number of grains per panicle, 1000-grain weight and spikelet fertility percentage at genotypic and phenotypic levels. Thus, these traits could play pivotal role in the development of high yielding rice genotypes. Path coefficient analysis recorded highest positive direct effect on yield per plant through number of grains per panicle, filled grains weight per panicle, plant height and 1000-grain weight. Hence, traits like number of grains per panicle, filled grains weight per panicle and 1000-grain weight would be useful in future breeding programs to develop superior genotypes having excellent yield potential. [Naseer, S, Kashif M, Ahmad HM, Iqbal MS Ali Q. Estimation of genetic association among yield contributing ...
Most of the mutations described in human MDM2 are tolerated without significantly disrupting the ... more Most of the mutations described in human MDM2 are tolerated without significantly disrupting the corresponding structural or molecular function. However, some of them are associated with a variety of human diseases, including cancer. Numerous computational methods have been developed to predict the effects of missense single nucleotide variants (SNVs). The non-synonymous single nucleotide polymorphisms affect the function of XRCC1, which impairs the ability to repair DNA and therefore increases the risk of diseases such as cancer. In this study, sequence and structure-based computational tools were used to screen the total listed coding SNPs of the MDM2 gene in order to recognize and describe them. The potential 6 ns SNP of MDM2 were identified from 29 ns SNP by consistent analysis using computational tools PolyPhen 2, SIFT, PANTHER and cSNP. The computational methods were used to systematically classify functional mutations in the regulatory and coding regions that modify the expre...
1st International Conference on Applied Chemical, Biological and Agricultural Sciences
The present study was carried out to estimate interrelationship and genetic diversity in M3 gener... more The present study was carried out to estimate interrelationship and genetic diversity in M3 generation of Vigna radiata in response to gamma radiation. The experimental materials comprised the four irradiated lines of mungbean AUM-18, AUM-19, AUM-31 and M-2004 that were irradiated at 250 Gy, 350 Gy and 450 Gy doses of gamma rays. The experiment was laid out in split plot design, with two replications. Each replication was divided into main plot and subplot. Dose levels were assigned to main plots and varieties in subplots. High heritability estimates were observed for all traits. Phenotypic correlation of seed yield per plant with germination percentage and seeds per pod was positive and highly significant however positive and significant phenotypic correlation of clusters per plant and 100-seed weight while pods per plant was negative and significant. Higher heritability and significance correlation indicated that germination percentage, 100-seed weight and seeds per pod may be use...
Mongolian Medicinal Formula Anshen-Buxin-Liuwei Pill Alleviate Cardiomyocyte Hypoxia/Reoxygenation Injury via Mitochondrion Pathway
<jats:title>Abstract</jats:title> <jats:p>Anshen Buxin Liuwei pill (ABLP), a Mo... more <jats:title>Abstract</jats:title> <jats:p>Anshen Buxin Liuwei pill (ABLP), a Mongolian medicinal formula, composed of the six medicinal materials of Mongolian medicine <jats:italic>Bos taurus domesticus</jats:italic> Gmelin, <jats:italic>Choerospondias axillaris</jats:italic> (Roxb. ) Burtt et Hill, <jats:italic>Myristica fragrans</jats:italic> Houtt., <jats:italic>Eugenia caryophµllata</jats:italic> Thunb., <jats:italic>Aucklandia lappa</jats:italic> Decne., <jats:italic>Liqui dambar formosana</jats:italic> Hance, is considered to have a therapeutic effect on the symptoms such as coronary heart disease, angina pectoris, arrhythmia, depression and irritability, palpitation, and shortness of breath. Therefore, the present study was employed a network pharmacology approach to identify the potentially active ingredients and to evaluate the protective effect of ABLP on hypoxia/reoxygenation (HR)-induced H9c2 cardiomyocytes, and its influence on cell viability, apoptosis, oxidative stress. H9c2 cardiomyocytes were used to construct a HR injury model. CCK-8 assay and AnnexinV-FITC cell apoptosis assays were used for cell viability and cell apoptosis determination. The levels of LDH, SOD, MDA, CAT, CK, GSH-Px, Na<jats:sup>+</jats:sup>-K<jats:sup>+</jats:sup>-ATPase, and Ca<jats:sup>2+</jats:sup>-ATPase in the cells were determined to assess the effect of ABLP. the mRNA levels of Sirtuin3 (Sirt3) and Cytochrome C (Cytc) in H9c2 cells were determined by quantitative real-time PCR. The finding of this study indicates that HR treatment cells began to shrink from the spindle in an irregular shape with some floated in the medium, well by increasing the therapeutic dose of ABLP (5 µg/mL, 25 µg/mL, and 50 µg/mL), the cells gradually reconverted in a concentration-dependent manner. The release of CK in HR-treated cells was significantly increased, indicating that ABLP exerts a protective effect in H9c2 cells against HR injury and can improve the mitochondrial energy metabolism and mitochondrial function integrity. The present study scrutinized the cardio-protective effect of ABLP against the HR-induced H9c2 cells injury through antioxidant and mitochondrion pathways. ABLP could be a promising therapeutic drug for the treatment of myocardial ischemic cardiovascular disease. The results will provide reasonable information for clinical use of the ABLP.</jats:p>
Evidence-Based Complementary and Alternative Medicine
Background. Anshen Buxin Liuwei pill (ABLP) is a Mongolian medicinal formula which has a therapeu... more Background. Anshen Buxin Liuwei pill (ABLP) is a Mongolian medicinal formula which has a therapeutic effect on the symptoms such as coronary heart disease, angina pectoris, arrhythmia, depression and irritability, palpitation, and short breath. However, its bioactivity against cardiac injury remains unclear. Methods. The protective effect of ABLP was evaluated using H9c2 cells. Cell viability, intracellular Ca2+, reactive oxidative indices, and mitochondrial membrane potential (∆ψ) were assessed, respectively. The mRNA levels of Ca2+ channel-related genes (DHPR, RyR2, and SCN5A) and oxidative stress-related genes (Keap1, Nrf2, and HO-1) were measured by RT-PCR. Results. 0.5–50 μg/mL ABLP could significantly decrease H2O2-induced cell injury by suppressing cell necrosis/apoptosis and excess oxidative stress, ameliorating the collapse of ∆ψ, and reducing intracellular Ca2+ concentration. Furthermore, 0.5–50 μg/mL ABLP reversed H2O2-induced imbalance in the mRNA levels of DHPR, RyR2, S...
The present study was carried out to estimate inter relationship and genetic diversity in M 3 gen... more The present study was carried out to estimate inter relationship and genetic diversity in M 3 generation of Vigna radiata in response to gamma radiation. The experimental m aterials comprised the four irradiated lines of mungbean AUM-18, AUM-19, AUM-31 and M-2004 that wer e i radiated at 250 Gy, 350 Gy and 450 Gy doses of gamma rays. The experiment was laid out in split pl o design, with two replications. Each replication was divided into main plot and subplot. Dose levels were assign ed to main plots and varieties in subplots. High he ritability estimates were observed for all traits. Phenotypic correlation of seed yield per plant with germinatio n percentage and seeds per pod was positive and highly significant h owever positive and significant phenotypic correlat ion of clusters per plant and 100-seed weight while pods per plant was negative and significant. Higher heritability a nd significance correlation indicated that germination percentage, 100-seed weight and seeds per pod ...
Toll-like receptors (TLRs) are innate immune receptors that mediate the inflammatory response dur... more Toll-like receptors (TLRs) are innate immune receptors that mediate the inflammatory response during HCV infections. The goal of this study was to evaluate the association of TLR9 gene polymorphism (rs5743836) in Pakistani patients infected with genotype 3a of HCV. Total 500 subjects were recruited, 400 HCV patients and 100 healthy individuals. Genotyping of TLR9 (-1237T/C, rs5743836) was carried out in 400 HCV patients (323 interferon responders and 77 interferon non responder) and control group by applying High resolution melting (HRM) curve assay. No remarkable differences in distribution of genotype between HCV (p<0.0001; OR= 3.21, 95% CI= (2.514.12) and control groups (p<0.0001; OR=0.092, 95%CI= (0.0580.14) were observed. In conclusion TLR9-1237T/C gene polymorphism may not be considered as a molecular risk for patients with HCV in Pakistan.
Estimation of genetic association among yield contributing traits in aromatic and non-aromatic rice (Oryza sativa L) cultivars
This study provided useful evidences about yield and its contributing components on 8 morphologic... more This study provided useful evidences about yield and its contributing components on 8 morphological traits, which were studied in 24 Asian accessions of rice (Oryza sativa L.). Plant yield was positively and significantly correlated with filled grains weight per panicle, number of grains per panicle, 1000-grain weight and spikelet fertility percentage at genotypic and phenotypic levels. Thus, these traits could play pivotal role in the development of high yielding rice genotypes. Path coefficient analysis recorded highest positive direct effect on yield per plant through number of grains per panicle, filled grains weight per panicle, plant height and 1000-grain weight. Hence, traits like number of grains per panicle, filled grains weight per panicle and 1000-grain weight would be useful in future breeding programs to develop superior genotypes having excellent yield potential. [Naseer, S, Kashif M, Ahmad HM, Iqbal MS Ali Q. Estimation of genetic association among yield contributing ...
The experiment was conducted to reveal genetic variability and to study the parameter analysis in... more The experiment was conducted to reveal genetic variability and to study the parameter analysis in 24 aromatic and non-aromatic rice genotypes. Highly significant variations were determined and the phenotypic variance was relatively greater than genotypic variance for most of the traits, like plant height, number of spikelets per panicle and number of grains per panicle. Higher magnitude of phenotypic variance showed more effect of environment on expression of these traits. A few traits such as number of tillers per plant, spikelet fertility percentage, filled grains weight per panicle, 1000-grain weight and yield per plant indicated least differences between PV and GV, this phenomenon gave the information of gene control for expressivity of these traits. Highest GCV was observed for number of tillers per plant, then yield per plant, number of grains per panicle and filled grains weight per panicle, while spikelet fertility percentage showed lowest value of GCV. Sufficient amount of ...
Journal of Experimental Biology and Agricultural Sciences
The emergence of a novel coronavirus referred to as SARS-CoV-2 has become a global health apprehe... more The emergence of a novel coronavirus referred to as SARS-CoV-2 has become a global health apprehension due to rapid transmission tendency, severity, and wide geographical spread. This emergence was started from Wuhan, China in 2019 from the zoonotic source and spread worldwide, infecting almost half of the community on this earth. Many of the receptors are involved in proceeding with this infection in the organism's body. Toll-like receptors (TLRs) play essential and protective functions from a wide range of microbial pathogens. Small setup of TLR adaptor proteins leads to activate nuclear factor kappa B (NF-kB) and interferon-regulatory factor (IRF). Consequently, various advanced inflammatory cytokines, chemokines, and interferon reaction properties can be up-regulated. Similarly, TLR flagging works on autophagy in macrophages. Autophagy is a cell response to starvation that helps to eliminate damaged cytosol organelles and persistent proteins. It is also able to prevent the r...
Severe Acute Respiratory Syndrome Coronaviruses and 21ST Century Pandemic: An Overview of Functional Receptors and Challenge of Therapeutic Success
Journal of Experimental Biology and Agricultural Sciences
Sudden prevalence of coronavirus disease-2019 (COVID-19) has badly impeded and collapsed the emer... more Sudden prevalence of coronavirus disease-2019 (COVID-19) has badly impeded and collapsed the emerging global trend of economic development by its ongoing pandemic. The novel coronavirus also named severe acute respiratory syndrome virus – 2 (SARS-CoV-2) causes the disease COVID-19 that influences the health management of humans and the world commerce badly. It has affected the human social lives and education in underdeveloped countries and severely impeded industries, organizations, agriculture, etc. Three perceptible types of SARS-CoV-2 strains have been discovered. Each of them has specific receptors, and some of them are common in SARS and SARS-CoV-2. Among them, the ACE2 receptor is believed to be the central receptor of human infectious coronaviruses. It supports mainly to get access, enter into the cell, and causes the basic infection. Similarly, TMPRSS2 is also acting as a portal for a virus to get an approach to the cell and does not support metabolic processes like replica...
Evidence-Based Complementary and Alternative Medicine
Background. Anshen Buxin Liuwei pill (ABLP) is a Mongolian medicinal formula which has a therapeu... more Background. Anshen Buxin Liuwei pill (ABLP) is a Mongolian medicinal formula which has a therapeutic effect on the symptoms such as coronary heart disease, angina pectoris, arrhythmia, depression and irritability, palpitation, and short breath. However, its bioactivity against cardiac injury remains unclear. Methods. The protective effect of ABLP was evaluated using H9c2 cells. Cell viability, intracellular Ca2+, reactive oxidative indices, and mitochondrial membrane potential (∆ψ) were assessed, respectively. The mRNA levels of Ca2+ channel-related genes (DHPR, RyR2, and SCN5A) and oxidative stress-related genes (Keap1, Nrf2, and HO-1) were measured by RT-PCR. Results. 0.5–50 μg/mL ABLP could significantly decrease H2O2-induced cell injury by suppressing cell necrosis/apoptosis and excess oxidative stress, ameliorating the collapse of ∆ψ, and reducing intracellular Ca2+ concentration. Furthermore, 0.5–50 μg/mL ABLP reversed H2O2-induced imbalance in the mRNA levels of DHPR, RyR2, S...
The present study was carried out to estimate inter relationship and genetic diversity in M 3 gen... more The present study was carried out to estimate inter relationship and genetic diversity in M 3 generation of Vigna radiata in response to gamma radiation. The experimental m aterials comprised the four irradiated lines of mungbean AUM-18, AUM-19, AUM-31 and M-2004 that wer e i radiated at 250 Gy, 350 Gy and 450 Gy doses of gamma rays. The experiment was laid out in split pl o design, with two replications. Each replication was divided into main plot and subplot. Dose levels were assign ed to main plots and varieties in subplots. High he ritability estimates were observed for all traits. Phenotypic correlation of seed yield per plant with germinatio n percentage and seeds per pod was positive and highly significant h owever positive and significant phenotypic correlat ion of clusters per plant and 100-seed weight while pods per plant was negative and significant. Higher heritability a nd significance correlation indicated that germination percentage, 100-seed weight and seeds per pod ...
Toll-like receptors (TLRs) are innate immune receptors that mediate the inflammatory response dur... more Toll-like receptors (TLRs) are innate immune receptors that mediate the inflammatory response during HCV infections. The goal of this study was to evaluate the association of TLR9 gene polymorphism (rs5743836) in Pakistani patients infected with genotype 3a of HCV. Total 500 subjects were recruited, 400 HCV patients and 100 healthy individuals. Genotyping of TLR9 (-1237T/C, rs5743836) was carried out in 400 HCV patients (323 interferon responders and 77 interferon non responder) and control group by applying High resolution melting (HRM) curve assay. No remarkable differences in distribution of genotype between HCV (p<0.0001; OR= 3.21, 95% CI= (2.514.12) and control groups (p<0.0001; OR=0.092, 95%CI= (0.0580.14) were observed. In conclusion TLR9-1237T/C gene polymorphism may not be considered as a molecular risk for patients with HCV in Pakistan.
Estimation of genetic association among yield contributing traits in aromatic and non-aromatic rice (Oryza sativa L) cultivars
This study provided useful evidences about yield and its contributing components on 8 morphologic... more This study provided useful evidences about yield and its contributing components on 8 morphological traits, which were studied in 24 Asian accessions of rice (Oryza sativa L.). Plant yield was positively and significantly correlated with filled grains weight per panicle, number of grains per panicle, 1000-grain weight and spikelet fertility percentage at genotypic and phenotypic levels. Thus, these traits could play pivotal role in the development of high yielding rice genotypes. Path coefficient analysis recorded highest positive direct effect on yield per plant through number of grains per panicle, filled grains weight per panicle, plant height and 1000-grain weight. Hence, traits like number of grains per panicle, filled grains weight per panicle and 1000-grain weight would be useful in future breeding programs to develop superior genotypes having excellent yield potential. [Naseer, S, Kashif M, Ahmad HM, Iqbal MS Ali Q. Estimation of genetic association among yield contributing ...
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