Annali di igiene : medicina preventiva e di comunità
Electric arc welding is known to involve considerable exposure to extremely-low-frequency magneti... more Electric arc welding is known to involve considerable exposure to extremely-low-frequency magnetic fields (ELF-MF; 50 Hz). The aim of the present study was to evaluate individual exposure to ELF-MF during arc welding and to assess the eventually associated genotoxic hazard by evaluating primary DNA damage. The study group comprised 21 electric arc welders (exposed) and 21 non-exposed control subjects (healthy blood donors). Occupational exposure to ELF-MF was measured using personal dosimeters worn during one complete work-shift (7 am to 5 pm). The extent of primary DNA damage was measured in peripheral blood leukocytes with the standard procedure of the alkaline comet assay. Tail length showed to have similar values in welders and controls. Whereas, the data showed a significant decrease for tail intensity (p = 0.01) and tail moment (p = 0.02) counts in exposed subjects compared to controls. The different results of our present study and published investigations from other research...
Annali di igiene : medicina preventiva e di comunità
Metabolic syndrome (MetS) is a cluster of metabolic disorders that includes central obesity, hype... more Metabolic syndrome (MetS) is a cluster of metabolic disorders that includes central obesity, hyperinsulinemia, hypercholesterolemia, hypertriglyceridemia and high blood pressure (BP). Statistical reports suggest that the prevalence of MetS has dramatically increased during the recent years and is considered a worldwide epidemic. MetS has been found to be associated with increased risk of all-cause mortality, cardiovascular diseases (CVD), type 2 diabetes mellitus, chronic kidney disease and some types of cancer. MetS has a high socioeconomic cost and it is therefore extremely important that MetS is prevented and treated by simple and feasible methods. The PreveDi study is a pilot before/after preventive trial aimed at the evaluation of the impact of a brief lifestyle intervention on changes in metabolic syndrome (MetS) risk factors. Recruitment was carried out in two community (council-run) pharmacies in the province of Perugia, Italy and the sample population consisted of 186 adult...
Herbal products such as herbal teas and plant food supplements (PFS) are widely used in Western d... more Herbal products such as herbal teas and plant food supplements (PFS) are widely used in Western diets. Although many consumers equal 'natural' with 'safe', botanical preparations may contain ingredients known to be toxic and even genotoxic or carcinogenic. One of the categories of plant metabolites of concern are the alkenylbenzenes, flavourings regulated by EU Regulation 1334/2008, used to improve or modify the odour and/or taste of several foods. trans-Anethole is a non-EU regulated flavouring substance present in the essential oils of a variety of plants, notably Foeniculum vulgare (fennel), Magnoliaceae (star anise) and Pimpinella anisum L.(anise).
Present-day society is increasingly demanding food products made of natural ingredients. While ma... more Present-day society is increasingly demanding food products made of natural ingredients. While many consumers equal 'natural' with 'safe', botanical preparations may contain ingredients known to be genotoxic or carcinogenic. Alkenylbenzenes, flavourings regulated by EU Reg. 1334/2008, are one of the categories of concern. trans-Cinnamaldehyde, a non-EU regulated alkenylbenzenic compound, is a constituent of the essential oils of Cinnamomum osmophloeum (cinnamon).
Il test della cometa in vitro su cellule polmonari umane (A549) è risultato in grado di evidenzia... more Il test della cometa in vitro su cellule polmonari umane (A549) è risultato in grado di evidenziare l'azione di genotossica di NP come TiO 2 e ZnO, mentre è risultato meno sensibile nell'evidenziare l'azione genotossica di NP e PSM raccolte nei comparti lavorativi in esame. Il test del micronucleo è invece risultato particolarmente sensibile nell'evidenziare la genotossicità del particolato campionato in ambito lavorativo. hanno avviato una collaborazione avente come obiettivo la definizione di una metodica, la più semplice e fruibile possibile, per caratterizzare gli effetti genotossici di NP e PSM presenti in alcuni ambienti di lavoro (nello specifico in aziende che svolgono lavori in sottosuolo e in aziende metalmeccaniche). L'applicabilità di tale metodica potrebbe consentire la segnalazione precoce della potenzialità patogena delle NP e permettere al medico competente di predisporre adeguati protocolli sanitari. 1. Introduzione. Le nanoparticelle (NP) sono, ...
The present study was undertaken to evaluate, in the HepG2 human hepatoma cell line, the in vitro... more The present study was undertaken to evaluate, in the HepG2 human hepatoma cell line, the in vitro cytotoxic, genotoxic, and apoptotic activities of estragole (1), contained in the essential oil of Foeniculum vulgare (fennel) and suspected to induce hepatic tumors in susceptible strains of mice. Toward this end, an MTT cytotoxicity assay, a trypan blue dye exclusion test, a double-staining (acridine orange and DAPI) fluorescence viability assay, a single-cell microgel-electrophoresis (comet) assay, a mitochondrial membrane potential (Δψm) assay, and a DNA fragmentation analysis were conducted. In terms of potential genotoxic effects, the comet assay indicated that estragole (1) was not able to induce DNA damage nor apoptosis under the experimental conditions used.
Annali di igiene : medicina preventiva e di comunità
Electric arc welding is known to involve considerable exposure to extremely-low-frequency magneti... more Electric arc welding is known to involve considerable exposure to extremely-low-frequency magnetic fields (ELF-MF; 50 Hz). The aim of the present study was to evaluate individual exposure to ELF-MF during arc welding and to assess the eventually associated genotoxic hazard by evaluating primary DNA damage. The study group comprised 21 electric arc welders (exposed) and 21 non-exposed control subjects (healthy blood donors). Occupational exposure to ELF-MF was measured using personal dosimeters worn during one complete work-shift (7 am to 5 pm). The extent of primary DNA damage was measured in peripheral blood leukocytes with the standard procedure of the alkaline comet assay. Tail length showed to have similar values in welders and controls. Whereas, the data showed a significant decrease for tail intensity (p = 0.01) and tail moment (p = 0.02) counts in exposed subjects compared to controls. The different results of our present study and published investigations from other research...
Annali di igiene : medicina preventiva e di comunità
Metabolic syndrome (MetS) is a cluster of metabolic disorders that includes central obesity, hype... more Metabolic syndrome (MetS) is a cluster of metabolic disorders that includes central obesity, hyperinsulinemia, hypercholesterolemia, hypertriglyceridemia and high blood pressure (BP). Statistical reports suggest that the prevalence of MetS has dramatically increased during the recent years and is considered a worldwide epidemic. MetS has been found to be associated with increased risk of all-cause mortality, cardiovascular diseases (CVD), type 2 diabetes mellitus, chronic kidney disease and some types of cancer. MetS has a high socioeconomic cost and it is therefore extremely important that MetS is prevented and treated by simple and feasible methods. The PreveDi study is a pilot before/after preventive trial aimed at the evaluation of the impact of a brief lifestyle intervention on changes in metabolic syndrome (MetS) risk factors. Recruitment was carried out in two community (council-run) pharmacies in the province of Perugia, Italy and the sample population consisted of 186 adult...
Herbal products such as herbal teas and plant food supplements (PFS) are widely used in Western d... more Herbal products such as herbal teas and plant food supplements (PFS) are widely used in Western diets. Although many consumers equal 'natural' with 'safe', botanical preparations may contain ingredients known to be toxic and even genotoxic or carcinogenic. One of the categories of plant metabolites of concern are the alkenylbenzenes, flavourings regulated by EU Regulation 1334/2008, used to improve or modify the odour and/or taste of several foods. trans-Anethole is a non-EU regulated flavouring substance present in the essential oils of a variety of plants, notably Foeniculum vulgare (fennel), Magnoliaceae (star anise) and Pimpinella anisum L.(anise).
Present-day society is increasingly demanding food products made of natural ingredients. While ma... more Present-day society is increasingly demanding food products made of natural ingredients. While many consumers equal 'natural' with 'safe', botanical preparations may contain ingredients known to be genotoxic or carcinogenic. Alkenylbenzenes, flavourings regulated by EU Reg. 1334/2008, are one of the categories of concern. trans-Cinnamaldehyde, a non-EU regulated alkenylbenzenic compound, is a constituent of the essential oils of Cinnamomum osmophloeum (cinnamon).
Il test della cometa in vitro su cellule polmonari umane (A549) è risultato in grado di evidenzia... more Il test della cometa in vitro su cellule polmonari umane (A549) è risultato in grado di evidenziare l'azione di genotossica di NP come TiO 2 e ZnO, mentre è risultato meno sensibile nell'evidenziare l'azione genotossica di NP e PSM raccolte nei comparti lavorativi in esame. Il test del micronucleo è invece risultato particolarmente sensibile nell'evidenziare la genotossicità del particolato campionato in ambito lavorativo. hanno avviato una collaborazione avente come obiettivo la definizione di una metodica, la più semplice e fruibile possibile, per caratterizzare gli effetti genotossici di NP e PSM presenti in alcuni ambienti di lavoro (nello specifico in aziende che svolgono lavori in sottosuolo e in aziende metalmeccaniche). L'applicabilità di tale metodica potrebbe consentire la segnalazione precoce della potenzialità patogena delle NP e permettere al medico competente di predisporre adeguati protocolli sanitari. 1. Introduzione. Le nanoparticelle (NP) sono, ...
The present study was undertaken to evaluate, in the HepG2 human hepatoma cell line, the in vitro... more The present study was undertaken to evaluate, in the HepG2 human hepatoma cell line, the in vitro cytotoxic, genotoxic, and apoptotic activities of estragole (1), contained in the essential oil of Foeniculum vulgare (fennel) and suspected to induce hepatic tumors in susceptible strains of mice. Toward this end, an MTT cytotoxicity assay, a trypan blue dye exclusion test, a double-staining (acridine orange and DAPI) fluorescence viability assay, a single-cell microgel-electrophoresis (comet) assay, a mitochondrial membrane potential (Δψm) assay, and a DNA fragmentation analysis were conducted. In terms of potential genotoxic effects, the comet assay indicated that estragole (1) was not able to induce DNA damage nor apoptosis under the experimental conditions used.
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