Root Biology - Growth, Physiology, and Functions [Working Title]
The translocation of nitrate in intact plant of barley (Hordeum vulgare L. cv.) two genotypes, wi... more The translocation of nitrate in intact plant of barley (Hordeum vulgare L. cv.) two genotypes, wild type Steptoe and a mutant Az12, was visualized by a positron-emitting tracer imaging system (PETIS) after supplying positron-emitting 13 N-labelled nitrate (13 NO 3 −) to the seedlings. 13 N movement was monitored to visualize the distribution of radioactivity in the two genotypes. N sufficient seedlings causes enhanced N uptake and translocation to shoots in time course from (0, 10, 20, 30, 40 min). The N-depleted seedlings were exposed to a nutrient solution containing nitrate and nitrite, and were labeled with 15 N for 38 h under (14L/10D) cycles. The two genotypes utilized 15 NO 3 − and accumulated it as reduced 15 N, predominately in the shoots. In the Az12, nitrate accumulation in shoots was 78% higher than that in the Steptoe. Accumulation of reduced 15 N in the Az12 roots was nearly similar to that of the Steptoe roots, but 8% lower in the Az12 shoots than in the Steptoe shoots at the end of the experiment.
Root Biology - Growth, Physiology, and Functions [Working Title]
The translocation of nitrate in intact plant of barley (Hordeum vulgare L. cv.) two genotypes, wi... more The translocation of nitrate in intact plant of barley (Hordeum vulgare L. cv.) two genotypes, wild type Steptoe and a mutant Az12, was visualized by a positron-emitting tracer imaging system (PETIS) after supplying positron-emitting 13 N-labelled nitrate (13 NO 3 −) to the seedlings. 13 N movement was monitored to visualize the distribution of radioactivity in the two genotypes. N sufficient seedlings causes enhanced N uptake and translocation to shoots in time course from (0, 10, 20, 30, 40 min). The N-depleted seedlings were exposed to a nutrient solution containing nitrate and nitrite, and were labeled with 15 N for 38 h under (14L/10D) cycles. The two genotypes utilized 15 NO 3 − and accumulated it as reduced 15 N, predominately in the shoots. In the Az12, nitrate accumulation in shoots was 78% higher than that in the Steptoe. Accumulation of reduced 15 N in the Az12 roots was nearly similar to that of the Steptoe roots, but 8% lower in the Az12 shoots than in the Steptoe shoots at the end of the experiment.
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