Papers by Gabriel Salerni
Reflectance confocal microscopy (RCM) shows the morphology of various skin layers according to th... more Reflectance confocal microscopy (RCM) shows the morphology of various skin layers according to the specific architectural and cytologic features characteristic of each layer. In this chapter, the morphology of skin layers of normal skin under confocal microscopy will be covered.
Dermatology practical & conceptual, Jan 31, 2023
International Journal of Women's Dermatology, Jun 1, 2020
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
El presente trabajo reune 3 articulos, que en su conjunto tienen por objetivo la validacion del s... more El presente trabajo reune 3 articulos, que en su conjunto tienen por objetivo la validacion del seguimiento a largo plazo de pacientes de riesgo para melanoma con el uso combinado de fotografia corporal total y dermatoscopia digital, llamado ?procedimiento de dos pasos del seguimiento digital?. En un primer articulo, se analizan las caracteristicas clinicas, dermatoscopicas e histologicas de 215 melanomas vistos de forma consecutiva en una unidad especializada en melanoma, 52 diagnosticados en pacientes en seguimiento en dicha unidad, y 163 derivados para valoracion con sospecha clinica de melanoma. El objetivo del estudio era comparar las caracteristicas clinicas, dermatoscopicas e histologicas de los melanomas en ambos grupos. En este estudio demostramos que la inclusion de pacientes de alto riesgo para el melanoma en programas de seguimiento permite la deteccion de melanomas en etapas iniciales y con buen pronostico, incluso en el ausencia de caracteristicas clinicas y dermatoscopicas de melanoma. En la poblacion general, sin una vigilancia especifica, el melanoma continua siendo diagnosticado en etapas avanzadas. Los individuos de alto riesgo, siempre que los recursos adecuados esten disponibles, deberian ser enviados a centros de melanoma o instituciones competentes para un seguimiento regular. En un segundo articulo, se analiza la experiencia de 10 anos en el seguimiento de 618 pacientes de alto riesgo de desarrollar melanoma incluidos en un programa especifico de seguimiento, con el objetivo de reportar los beneficios del uso combinado de fotografia corporal total y dermatoscopia digital (metodo de dos pasos de seguimiento digital) en el diagnostico precoz de melanoma. El uso de fotografia corporal total y dermatoscopia digital en una poblacion de alto riesgo para el melanoma permite la deteccion de melanomas etapas iniciales con una baja tasa de extirpaciones. Esta modalidad dual permite la deteccion de melanomas con pocos criterios dermatoscopicos en base a cambios microscopicos en seguimiento digital asi como la deteccion de melanomas que se presentan como lesiones nuevas o a partir de lesiones sin registro dermatoscopico previo. Largos periodos de seguimiento son necesarios para permitir la deteccion de melanomas de lento crecimiento. En base a nuestra experiencia de 10 anos, los melanomas pueden ser diagnosticados en cualquier momento, y no solo al principio del seguimiento, lo que sugiere que en poblaciones de alto riesgo el seguimiento digital debe ser mantenido en el tiempo Finalmente, en un tercer articulo, se analizan 1152 lesiones extirpadas a lo largo de 10 anos de seguimiento de los 618 pacientes de alto riesgo para desarrollar melanoma incluidos en el trabajo anterior, con el objetivo de identificar las caracteristicas dermatoscopicas y los cambios dinamicos que condujeron a la extirpacion de dichas lesiones. En este trabajo demostramos que los hallazgos dermatoscopicos mas frecuentemente asociados con melanoma fueron cambios focales en pigmentacion y cambios focales en estructura. Los melanomas detectados por cambios dermatoscopicos fueron muy similares a los detectados gracias al uso de fotografia corporal total, con un porcentaje considerable en ambos grupos de melanomas clasificados como lesiones benignas. Casi el 40% de los melanomas diagnosticados correspondieron a lesiones que no tenian registro dermatoscopico previo. Todos los nevus atipicos que muestren modificaciones sustanciales en el tiempo debe ser extirpados para no pasar por alto un melanoma.
Journal of The American Academy of Dermatology, Jun 1, 2021
Springer eBooks, Nov 28, 2011
ABSTRACT Confocal microscopy has been used in the study of nonmelanocytic tumors since the begin... more ABSTRACT Confocal microscopy has been used in the study of nonmelanocytic tumors since the beginning of the technology [1]. Numerous publications have provided criteria and descriptors for different neoplasms such as basal cell carcinoma, keratinizing tumors, angioma and dermatofibroma among others. The limits of resolution and depth of the microscope make impossible the observation of the tumor component in the reticular dermis and hypodermis and therefore RCM is not useful for the study of the deep component of thick lesions of the skin. Likewise RCM cannot provide reliable information in deep recurrences or surgical margins in the case of carcinoma prior to surgery [2]. Moreover, RCM can be very sensible and specific in the detection of the superficial part of the tumor that is sufficient for a correct diagnosis in many cutaneous neoplasms. In the case of basal cell carcinoma (BCC) and squamous cell carcinoma (SCC), different application of RCM in the ex vivo modality with the use of specific staining products for RCM such as acridine has been reported. Ex vivo RCM has been postulated to be useful in MOHS surgery in theses tumors [3].
Dermatología Revista Mexicana, 2012
Dermatología argentina, Jun 10, 2011
en dermatología Use of confocal reflectance microscopy in dermatology RESUMEN La microscopia conf... more en dermatología Use of confocal reflectance microscopy in dermatology RESUMEN La microscopia confocal de reflectancia (MCR) es una herramienta de imágenes no invasiva que permite la visualización in vivo y en tiempo real de estructuras microscópicas de la epidermis, unión dermo-epidérmica y dermis superficial con una resolución cuasi histológica. Ha sido utilizada en la evaluación de lesiones benignas y malignas, demostrando un gran potencial para aplicaciones en investigación básica y en dermatología clínica. En el presente trabajo se revisan conceptos acerca del desarrollo y principios básicos del funcionamiento de la MCR, así como de sus aplicaciones en la práctica clínica (Dermatol. Argent., 2011, 17(3): 230-235).
Dermatology practical & conceptual, Feb 2, 2022
Dermatology Practical & Conceptual
Introduction: Dermoscopy is a useful technique that aids in early detection of skin cancer by inc... more Introduction: Dermoscopy is a useful technique that aids in early detection of skin cancer by increasing diagnostic accuracy with adequate training. However, dermoscopy is not uniformly taught to residents worldwide. Dermoscopy training in Latin American dermatology residency programs has not been explored. Objectives: To assess current dermoscopy training among dermatology residency programs in Latin America (e.g. training modalities, preferred/most effective modalities per residents, diseases/pathologies taught). Methods: Cross-sectional survey distributed via email between March and May 2021. Chief residents from Argentina, Brazil, Colombia, Costa Rica, Chile, Ecuador, Guatemala, Mexico, Panama, and Uruguay were invited to participate. Results: 81 chief residents completed the questionnaire (81/126, 64.2%). 72% of programs had an established dermoscopy curriculum, with dedicated hours of training varying greatly by program. Institutions commonly utilized sessions with “unknown” d...
Dermatology practical & conceptual, Jan 31, 2023
Conflicto de interés: los autores declaran que no existe conflicto de interés.
Dermatology, 2008
Access to full text and tables of contents, including tentative ones for forthcoming issues: www.... more Access to full text and tables of contents, including tentative ones for forthcoming issues: www.karger.com/drm_issues 58 Papillon-Lefèvre Syndrome and Malignant Melanoma. A High Incidence of Melanoma Development in Japanese Palmoplantar Keratoderma Patients
Dermatology Practical & Conceptual, 2015
Background: In vivo confocal microscopy is an imaging technique that has been applied to the stud... more Background: In vivo confocal microscopy is an imaging technique that has been applied to the study of the ocular surface. However, confocal microscopes dedicated to eye examination are routinely adopted only in ophthalmology reference centres and do not allow an examination of periocular tissue, nor a fluorescence examination. Methods: We applied for the first time the two in vivo confocal microscopes commonly used in dermatology (VivaScope ® 1500 and 3000, CALIBER, distributed in Europe by Mavig GmbH, Munich, Germany) to observe the cornea, the bulbar and tarsal conjunctiva, the eyelid margin, the lacrimal punctum and the palpebral skin of healthy volunteers. Tumoral, inflammatory and infectious diseases of the ocular mucosa and periocular skin from more than 200 patients were observed under the same microscopes. Both microscopes have a reflectance mode. VivaScope ® 1500 allows an additional fluorescent examination and its placement on the ocular surface was made possible by the creation of a special interface between the microscope and the ocular apparatus. Results: Thanks to its compact and flexible configuration, the handheld camera VivaScope ® 3000 allowed to access more easily to the ocular and periocular tissues. Diagnosis of benign and malignant tumors (melanoma, squamous cell carcinoma and basal cell carcinoma), as well as infectious and genetic disease (storage diseases), could be evocated. The detection of parasites (Demodex folliculorum) on eyelids was possible. Confocal images correlated well with conventional histopathology. The fluorescence examination of corneal squamous cell carcinoma by VivaScope 1500 was characterized by extravasation of fluorescein after intravenous injection. Conclusions: Confocal microscopes dedicated to the skin offer new perspectives for the diagnosis, optimization of treatments, and follow-up of the ocular diseases. They will allow dermatologists to examine conjunctival and eyelid tumors, as it is for skin or genital mucosa. In addition, thanks to some adaptations of the dermatological device VivaScope ® 1500, it is possible for the first time to perform a fluorsecnte examination of the ocular and peri-ocular tissue, opening a new era in the clinical imaging of the ocular surface. A new semiology remains to be learned.
Journal of Cosmetic Dermatology, 2021
BackgroundDiagnosis of pigmentary skin disorders, pre‐cancerous and cancerous skin diseases is tr... more BackgroundDiagnosis of pigmentary skin disorders, pre‐cancerous and cancerous skin diseases is traditionally relied on visual assessment. The most widely applied invasive diagnostic technique is the skin biopsy. There have been significant technological advances in non‐invasive diagnostic methods for skin disorders.ObjectiveThe objective of this article is to discuss different non‐invasive diagnostic modalities, used in the diagnosis of pigmentary skin disorders and cutaneous cancers.MethodsComprehensive literature search was performed to screen articles related to non‐invasive diagnostic techniques in pigmentary skin disorders and cutaneous cancers. Articles published in journals indexed in PubMed were searched along with those in Google Scholar. Clinical trials, review articles, case series, case reports and other relevant articles were considered for review. References of relevant articles were also considered for review.ResultsDermoscopy and ultrasonography were the only non‐inv...
Journal of Cosmetic Dermatology, 2021
BackgroundMelanoma is associated with poor prognosis in its advanced stages. Potential influence ... more BackgroundMelanoma is associated with poor prognosis in its advanced stages. Potential influence of estrogen and its metabolites on melanoma growth has been suggested.AimsThe objective of this review was to provide an overview on the evidence related to estrogen in malignant melanoma.Materials and MethodsLiterature search using PubMed, Google Scholar and relevant cross‐references of the retrieved articles was performed to review relevant published articles related to estrogen and its effects in malignant melanoma.ResultsEffect of estrogen signaling on a tissue largely depends on the relative expression of estrogen receptors (ER) α and β. Gender differences in melanoma may be explained by the difference in expression of these receptors. ERβ is the principal ER in melanoma.DiscussionAlthough there is uncertainty about role of estrogen in pathogenensis and progression of melanoma, evidence suggests that its growth and metastasis are influenced by estrogen stimulation. Role ER on the pr...
Clinical and Experimental Dermatology, 2020
Integrative Cancer Science and Therapeutics, 2016
Our secondary objective was to note the survival according to lymph node biopsy results.
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Papers by Gabriel Salerni