PTEN gene is refer to Phosphatase and TENs in homolog deleted on chromosome 10 which is a tumor s... more PTEN gene is refer to Phosphatase and TENs in homolog deleted on chromosome 10 which is a tumor suppressor gene located at chromosome 10q23.31, encoding for protein that have both lipid and protein phosphatase activities. The essential function of PTEN is to block the PI3K pathway by dephosphorylating phosphatidylinositol (PI) 3,4,5-triphosphate to PI-4,5-bisphosphate thus neutralize PI3K function. Genetic alterations of the genes related to PI3K/Akt pathway, including mutations of PI3K and PTEN, facilitate tumor genesis and are common in human cancers. The aim of this study is to assess a molecular analysis of PTEN gene in some colorectal Iraqi patients. Twenty-two patients, 11 colorectal cancers and 11 with bowel inflammation from Iraqi patients were screened in exon 7 of PTEN gene using PCR and sequencing analysis. The results showed 23% (3 out of 22) patients were detected mutations. All 45% (10 out of 22) PTEN mutations in 3 colorectal patients were single base substitutions. In this study there are 60% (6 out of 10) transition mutations, 30% (3out of 10) transversion mutations and 10% (1 out of 10) insertion mutation. In conclusion PTEN gene mutations may cause the etiology of CRC as there were genetic alterations in bowel inflammation and colorectal patients.
Background:Time attendance fingerprint device became used increasingly in many of government ins... more Background:Time attendance fingerprint device became used increasingly in many of government institutions such as universities , hospitals and airports. In such crowded places the chance of transmission the transitional diseases are fairly large. Verocytotoxigenic E. coli is one of pathogenic bacteria that can transfer by direct or indirect contact with few numbers and succeeded in causing infection. Objective:This paper aim to study and evaluation the possibility of transmission of bacterial infection via fingerprinting and detection of Verotoxic Escherichia coli using conventional and rapid PCR techniques. Results :Twenty-four cotton swab collected from the surface of 12 fingerprint device used in three hospitals and two universities in Baghdad city. Conventional and Rapid PCR techniques were used for detection of Verotoxic Escherichia coli. Results showed that 66.6 % (4) of swabs which collected from 6 devices from 3 hospitals and tested for VTEC using rapid PCR technique were positive. While only 33.3% (2) of swabs tested by conventional PCR technique showed positive detection for VTEC. All the samples that were taken from the surfaces of fingerprint devices in universities were free of bacteria and the same results were obtained from using the conventional and rapid PCR techniques.In conclusion: The fingerprint device which currently used in different places is associated with a risk of infection transmission of Verocytotoxigenic E. coli and the rapid PCR technique is preferred for the detection of these bacteria because it easy , short time , do not need to use additional steps or kits for DNA extraction with low cost compared to conventional method.
PTEN gene is refer to Phosphatase and TENs in homolog deleted on chromosome 10 which is a tumor s... more PTEN gene is refer to Phosphatase and TENs in homolog deleted on chromosome 10 which is a tumor suppressor gene located at chromosome 10q23.31, encoding for protein that have both lipid and protein phosphatase activities. The essential function of PTEN is to block the PI3K pathway by dephosphorylating phosphatidylinositol (PI) 3,4,5-triphosphate to PI-4,5-bisphosphate thus neutralize PI3K function. Genetic alterations of the genes related to PI3K/Akt pathway, including mutations of PI3K and PTEN, facilitate tumor genesis and are common in human cancers. The aim of this study is to assess a molecular analysis of PTEN gene in some colorectal Iraqi patients. Twenty-two patients, 11 colorectal cancers and 11 with bowel inflammation from Iraqi patients were screened in exon 7 of PTEN gene using PCR and sequencing analysis. The results showed 23% (3 out of 22) patients were detected mutations. All 45% (10 out of 22) PTEN mutations in 3 colorectal patients were single base substitutions. In this study there are 60% (6 out of 10) transition mutations, 30% (3out of 10) transversion mutations and 10% (1 out of 10) insertion mutation. In conclusion PTEN gene mutations may cause the etiology of CRC as there were genetic alterations in bowel inflammation and colorectal patients.
A mitochondria have important role in oxidative phosphorylation, each cell have many copies of mi... more A mitochondria have important role in oxidative phosphorylation, each cell have many copies of mitochondria. Mitochondrial DNA (mtDNA) in human is a 16. 569 kb circular double stranded molecule able for replication, transcription and translation. MtDNA represent the unique genetic material outside the nucleus in eukaryotic cells. This study was conduct on 36 Iraqi patients with colorectal disease to analyze the genetic mutation in A12308G of mtDNA tRNA Leu(CUN) gene for its etiology role in inflammation bowel and colorectal tumorgenesis using conventional polymerase chain reaction (PCR) and sequencing analysis. The results referred to that total frequency of the A12308G transition was showed 6 variations out of 18 patients (33.3%) in colorectal patients but these differences were not statistically significant, similar distribution for the frequency of the G12372A transition mutations was found in combination with A12308G polymorphism patients .Total of 7 out 18 patients (38.8%) were presence as insertion (ins) base between (12413-12414). Statistically significant results with high rate of deletion 11924A base was showed in 10 out of 18 samples (55.5%), coexisted of A12308G with G12372A mutations in addition to that in combination of A12410 C with T12414A may have synergistic effect in functions and associated with risk factor in colorectal cancer, finally large deletion site in (12380...12425) and (12383...12425) positions were described only in two tumor patients may be effect the colorectal cancer progression. In conclusions: The mtDNA A12308G tRNA Leu(CUN) gene variant may be don't have risk factor for malignant process of colorectal. Identify the predictive role of the mt tRNA Leu (CUN) gene variations may be use as genetic biomarker in colorectal cancer, high rate of novel mutations that were found may serve as supporting factor in colorectal etiology and tumorgensis.
PTEN gene is refer to Phosphatase and TENs in homolog deleted on chromosome 10 which is a tumor s... more PTEN gene is refer to Phosphatase and TENs in homolog deleted on chromosome 10 which is a tumor suppressor gene located at chromosome 10q23.31, encoding for protein that have both lipid and protein phosphatase activities. The essential function of PTEN is to block the PI3K pathway by dephosphorylating phosphatidylinositol (PI) 3,4,5-triphosphate to PI-4,5-bisphosphate thus neutralize PI3K function. Genetic alterations of the genes related to PI3K/Akt pathway, including mutations of PI3K and PTEN, facilitate tumor genesis and are common in human cancers. The aim of this study is to assess a molecular analysis of PTEN gene in some colorectal Iraqi patients. Twenty-two patients, 11 colorectal cancers and 11 with bowel inflammation from Iraqi patients were screened in exon 7 of PTEN gene using PCR and sequencing analysis. The results showed 23% (3 out of 22) patients were detected mutations. All 45% (10 out of 22) PTEN mutations in 3 colorectal patients were single base substitutions. In this study there are 60% (6 out of 10) transition mutations, 30% (3out of 10) transversion mutations and 10% (1 out of 10) insertion mutation. In conclusion PTEN gene mutations may cause the etiology of CRC as there were genetic alterations in bowel inflammation and colorectal patients.
Background:Time attendance fingerprint device became used increasingly in many of government ins... more Background:Time attendance fingerprint device became used increasingly in many of government institutions such as universities , hospitals and airports. In such crowded places the chance of transmission the transitional diseases are fairly large. Verocytotoxigenic E. coli is one of pathogenic bacteria that can transfer by direct or indirect contact with few numbers and succeeded in causing infection. Objective:This paper aim to study and evaluation the possibility of transmission of bacterial infection via fingerprinting and detection of Verotoxic Escherichia coli using conventional and rapid PCR techniques. Results :Twenty-four cotton swab collected from the surface of 12 fingerprint device used in three hospitals and two universities in Baghdad city. Conventional and Rapid PCR techniques were used for detection of Verotoxic Escherichia coli. Results showed that 66.6 % (4) of swabs which collected from 6 devices from 3 hospitals and tested for VTEC using rapid PCR technique were positive. While only 33.3% (2) of swabs tested by conventional PCR technique showed positive detection for VTEC. All the samples that were taken from the surfaces of fingerprint devices in universities were free of bacteria and the same results were obtained from using the conventional and rapid PCR techniques.In conclusion: The fingerprint device which currently used in different places is associated with a risk of infection transmission of Verocytotoxigenic E. coli and the rapid PCR technique is preferred for the detection of these bacteria because it easy , short time , do not need to use additional steps or kits for DNA extraction with low cost compared to conventional method.
PTEN gene is refer to Phosphatase and TENs in homolog deleted on chromosome 10 which is a tumor s... more PTEN gene is refer to Phosphatase and TENs in homolog deleted on chromosome 10 which is a tumor suppressor gene located at chromosome 10q23.31, encoding for protein that have both lipid and protein phosphatase activities. The essential function of PTEN is to block the PI3K pathway by dephosphorylating phosphatidylinositol (PI) 3,4,5-triphosphate to PI-4,5-bisphosphate thus neutralize PI3K function. Genetic alterations of the genes related to PI3K/Akt pathway, including mutations of PI3K and PTEN, facilitate tumor genesis and are common in human cancers. The aim of this study is to assess a molecular analysis of PTEN gene in some colorectal Iraqi patients. Twenty-two patients, 11 colorectal cancers and 11 with bowel inflammation from Iraqi patients were screened in exon 7 of PTEN gene using PCR and sequencing analysis. The results showed 23% (3 out of 22) patients were detected mutations. All 45% (10 out of 22) PTEN mutations in 3 colorectal patients were single base substitutions. In this study there are 60% (6 out of 10) transition mutations, 30% (3out of 10) transversion mutations and 10% (1 out of 10) insertion mutation. In conclusion PTEN gene mutations may cause the etiology of CRC as there were genetic alterations in bowel inflammation and colorectal patients.
A mitochondria have important role in oxidative phosphorylation, each cell have many copies of mi... more A mitochondria have important role in oxidative phosphorylation, each cell have many copies of mitochondria. Mitochondrial DNA (mtDNA) in human is a 16. 569 kb circular double stranded molecule able for replication, transcription and translation. MtDNA represent the unique genetic material outside the nucleus in eukaryotic cells. This study was conduct on 36 Iraqi patients with colorectal disease to analyze the genetic mutation in A12308G of mtDNA tRNA Leu(CUN) gene for its etiology role in inflammation bowel and colorectal tumorgenesis using conventional polymerase chain reaction (PCR) and sequencing analysis. The results referred to that total frequency of the A12308G transition was showed 6 variations out of 18 patients (33.3%) in colorectal patients but these differences were not statistically significant, similar distribution for the frequency of the G12372A transition mutations was found in combination with A12308G polymorphism patients .Total of 7 out 18 patients (38.8%) were presence as insertion (ins) base between (12413-12414). Statistically significant results with high rate of deletion 11924A base was showed in 10 out of 18 samples (55.5%), coexisted of A12308G with G12372A mutations in addition to that in combination of A12410 C with T12414A may have synergistic effect in functions and associated with risk factor in colorectal cancer, finally large deletion site in (12380...12425) and (12383...12425) positions were described only in two tumor patients may be effect the colorectal cancer progression. In conclusions: The mtDNA A12308G tRNA Leu(CUN) gene variant may be don't have risk factor for malignant process of colorectal. Identify the predictive role of the mt tRNA Leu (CUN) gene variations may be use as genetic biomarker in colorectal cancer, high rate of novel mutations that were found may serve as supporting factor in colorectal etiology and tumorgensis.
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