It is with a great sadness and a heavy heart that we learnt a few weeks ago that our friend and c... more It is with a great sadness and a heavy heart that we learnt a few weeks ago that our friend and colleague, Prof. Dr. Geert-Jan de Klerk, who had also been an EiC of PCTOC passed away last February 8, 2023, and his funeral was held on February 15, 2023, in The Netherlands.
Acknowledgement of referees Agricultural and Food Science expresses its sincere thanks to the fol... more Acknowledgement of referees Agricultural and Food Science expresses its sincere thanks to the following referees for their constructive critical reviews of one or more manuscripts during the year 2011.
The WEE1 kinase is ubiquitous in plant development and negatively regulates the cell cycle throug... more The WEE1 kinase is ubiquitous in plant development and negatively regulates the cell cycle through phosphorylations. However, analogies with the control of the human cell cycle by tyrosine- (Tyr-) phosphorylation of cyclin-dependent kinases (CDKs) are sometimes questioned. In this in silico study, we assessed the structural conservation of the WEE1 protein in the plant kingdom with a particular focus on agronomically valuable plants, the legume crops. We analyzed the phylogenetic distribution of amino-acid sequences among a large number of plants by Bayesian analysis that highlighted the general conservation of WEE1 proteins. A detailed sequence analysis confirmed the catalytic potential of WEE1 proteins in plants. However, some substitutions of an arginine and a glutamate at the entrance of the catalytic pocket, illustrated by 3D structure predictions, challenged the specificity of this protein toward the substrate and Tyr-phosphorylation compared to the human WEE1. The structural ...
Crinum malabaricum (Family: Amaryllidaceae) is a critically endangered aquatic medicinal plant en... more Crinum malabaricum (Family: Amaryllidaceae) is a critically endangered aquatic medicinal plant endemic to India. This species is a promising natural source of bioactive compounds including galanthamine (GAL), an anti-Alzheimer drug. In vitro regeneration in the Amaryllidaceae is often challenging. This study assessed the use of meta-Topolin (mT) on in vitro regeneration of C. malabaricum. Shoot explants were cultured on Murashige and Skoog (MS) medium supplemented with 0.5, 2.5, 5.0, 7.5 and 10.0 mM mT for six weeks, whereby 7.5 mM mT resulted in the maximum multiplication of adventitious shoots, much higher than the control. The biochemical accumulation of eleven different phenolic acids was quantified by UHPLC-MS/MS analysis, and it appeared that mT-treated cultures exhibited the highest concentration of phenolic acids. In particular, increased concentrations of gallic acid, protocatechuic acid, syringic acid, p-hydroxybenzoic acid, salicylic acid and vanillic acid were detected compared to the control. mT (2.5 and 5.0 mM) produced the maximum amount of chlorogenic acid, ferulic acid, p-coumaric acid and sinapic acid. However, an increased content of caffeic acid was produced on PGR-free medium. These findings highlight the beneficial effect and validate the rising importance of mT for in vitro regeneration studies. This study will serve as a potential protocol to conserve and restore the medically important C. malabaricum.
Medicinal plants are important for improving human health and represent an essential pool for the... more Medicinal plants are important for improving human health and represent an essential pool for the identifcation of novel pharmacological leads. Plant-derived biomolecules have historically proven their value as a source of therapeutic drugs and hold an important potential for the identifcation and characterization of novel drug leads. Many diferent alkaloids possessing a broad range of pharmacological activities have been isolated from plants belonging to the Amaryllidaceae family. Galanthamine (GAL) is a selective, reversible and an Amaryllidaceae-derived acetylcholinesterase inhibitor used for the treatment of Alzheimer’s disease (AD) and other neurological diseases. Naturally, the bioavailability of Amaryllidaceae alkaloids including GAL is low. Due to the signifcantly increased demand of GAL by the pharmaceutical industries and the inadequate availability of natural resources, in vitro culture ofers an alternative approach for its sustainable production. Thus, diferent biotechnological tools can optimize the in vitro GAL biosynthesis for treating AD, such as manipulation of plant growth regulators, photoperiod, elicitors, and bioreactors systems, besides being an environmentally sustainable approach, which protects the native biodiversity in a circular bioeconomy context. In the present review, we highlight the biosynthesis of GAL by plant in vitro systems including its mode of action. This article should also provide a starting point in the scaling-up of the biotechnological production of this valuable alkaloid.
The flow cytometry technique has been applied in order to check the ploidy level of seven provena... more The flow cytometry technique has been applied in order to check the ploidy level of seven provenances of local alfalfa (Medicago sativa L.) Gabsi, to estimate the genome (pg DNA) size of these alfalfa lines and to verify whether any genetic differences existed between these provenances belonging to the same population. Flow cytometry technique enabled us to show that all sources are tetraploid but also showed genetic variability that can be explained by the effect of microclimate, even if it is obvious that all these sources belong to a same unique population.
Journal of Horticultural Science and Biotechnology
Experiments were carried out to investigate the influence of combined nitrogen (N) and sulphur (S... more Experiments were carried out to investigate the influence of combined nitrogen (N) and sulphur (S) fertility on the organosulphur compound content of garlic bulbs, especially alliin, as a specific flavour quality trait related to the health-value of garlic. Three cultivars, 'Printanor', 'Morasol' and 'Messidr6me', were grown in the greenhouse and in vitro. Sulphur was increased in the macronutrients within the range 0 - 144 mg l(-1). Numerous combinations of both S and N were tested in vitro. In the greenhouse, increasing N levels, from 184.8 mg l(-1) to 856.8 mg l(-1), were tested with a single high level of S (128 mg l(-1)). Increasing S alone did not affect the growth or bulb weight of any garlic cultivar, but slightly increased the alliin content of bulbs. No symptoms of S deficiency were observed under greenhouse conditions, although the same garlic cultivars grown in vitro at 0 mg S l(-1) clearly suffered, their size being greatly reduced. This suggests...
This chapter provides a review of the current status of androgenesis and doubled-haploid producti... more This chapter provides a review of the current status of androgenesis and doubled-haploid production in the different food legumes (including chickpea, field pea, lentil, soyabean, common bean and lupin) and outlines some strategies to overcome this recalcitrance.
... Document details. Title The Lathyrus paradox: a "poor man's diet" or a remarka... more ... Document details. Title The Lathyrus paradox: a "poor man's diet" or a remarkable genetic resource for protein legume breeding? Authors Ochatt, SJ; Abirached-Darmency, M.; Marget, P.; Aubert, G. Editors Ochatt, S.;Jain, SM ...
It is with a great sadness and a heavy heart that we learnt a few weeks ago that our friend and c... more It is with a great sadness and a heavy heart that we learnt a few weeks ago that our friend and colleague, Prof. Dr. Geert-Jan de Klerk, who had also been an EiC of PCTOC passed away last February 8, 2023, and his funeral was held on February 15, 2023, in The Netherlands.
Acknowledgement of referees Agricultural and Food Science expresses its sincere thanks to the fol... more Acknowledgement of referees Agricultural and Food Science expresses its sincere thanks to the following referees for their constructive critical reviews of one or more manuscripts during the year 2011.
The WEE1 kinase is ubiquitous in plant development and negatively regulates the cell cycle throug... more The WEE1 kinase is ubiquitous in plant development and negatively regulates the cell cycle through phosphorylations. However, analogies with the control of the human cell cycle by tyrosine- (Tyr-) phosphorylation of cyclin-dependent kinases (CDKs) are sometimes questioned. In this in silico study, we assessed the structural conservation of the WEE1 protein in the plant kingdom with a particular focus on agronomically valuable plants, the legume crops. We analyzed the phylogenetic distribution of amino-acid sequences among a large number of plants by Bayesian analysis that highlighted the general conservation of WEE1 proteins. A detailed sequence analysis confirmed the catalytic potential of WEE1 proteins in plants. However, some substitutions of an arginine and a glutamate at the entrance of the catalytic pocket, illustrated by 3D structure predictions, challenged the specificity of this protein toward the substrate and Tyr-phosphorylation compared to the human WEE1. The structural ...
Crinum malabaricum (Family: Amaryllidaceae) is a critically endangered aquatic medicinal plant en... more Crinum malabaricum (Family: Amaryllidaceae) is a critically endangered aquatic medicinal plant endemic to India. This species is a promising natural source of bioactive compounds including galanthamine (GAL), an anti-Alzheimer drug. In vitro regeneration in the Amaryllidaceae is often challenging. This study assessed the use of meta-Topolin (mT) on in vitro regeneration of C. malabaricum. Shoot explants were cultured on Murashige and Skoog (MS) medium supplemented with 0.5, 2.5, 5.0, 7.5 and 10.0 mM mT for six weeks, whereby 7.5 mM mT resulted in the maximum multiplication of adventitious shoots, much higher than the control. The biochemical accumulation of eleven different phenolic acids was quantified by UHPLC-MS/MS analysis, and it appeared that mT-treated cultures exhibited the highest concentration of phenolic acids. In particular, increased concentrations of gallic acid, protocatechuic acid, syringic acid, p-hydroxybenzoic acid, salicylic acid and vanillic acid were detected compared to the control. mT (2.5 and 5.0 mM) produced the maximum amount of chlorogenic acid, ferulic acid, p-coumaric acid and sinapic acid. However, an increased content of caffeic acid was produced on PGR-free medium. These findings highlight the beneficial effect and validate the rising importance of mT for in vitro regeneration studies. This study will serve as a potential protocol to conserve and restore the medically important C. malabaricum.
Medicinal plants are important for improving human health and represent an essential pool for the... more Medicinal plants are important for improving human health and represent an essential pool for the identifcation of novel pharmacological leads. Plant-derived biomolecules have historically proven their value as a source of therapeutic drugs and hold an important potential for the identifcation and characterization of novel drug leads. Many diferent alkaloids possessing a broad range of pharmacological activities have been isolated from plants belonging to the Amaryllidaceae family. Galanthamine (GAL) is a selective, reversible and an Amaryllidaceae-derived acetylcholinesterase inhibitor used for the treatment of Alzheimer’s disease (AD) and other neurological diseases. Naturally, the bioavailability of Amaryllidaceae alkaloids including GAL is low. Due to the signifcantly increased demand of GAL by the pharmaceutical industries and the inadequate availability of natural resources, in vitro culture ofers an alternative approach for its sustainable production. Thus, diferent biotechnological tools can optimize the in vitro GAL biosynthesis for treating AD, such as manipulation of plant growth regulators, photoperiod, elicitors, and bioreactors systems, besides being an environmentally sustainable approach, which protects the native biodiversity in a circular bioeconomy context. In the present review, we highlight the biosynthesis of GAL by plant in vitro systems including its mode of action. This article should also provide a starting point in the scaling-up of the biotechnological production of this valuable alkaloid.
The flow cytometry technique has been applied in order to check the ploidy level of seven provena... more The flow cytometry technique has been applied in order to check the ploidy level of seven provenances of local alfalfa (Medicago sativa L.) Gabsi, to estimate the genome (pg DNA) size of these alfalfa lines and to verify whether any genetic differences existed between these provenances belonging to the same population. Flow cytometry technique enabled us to show that all sources are tetraploid but also showed genetic variability that can be explained by the effect of microclimate, even if it is obvious that all these sources belong to a same unique population.
Journal of Horticultural Science and Biotechnology
Experiments were carried out to investigate the influence of combined nitrogen (N) and sulphur (S... more Experiments were carried out to investigate the influence of combined nitrogen (N) and sulphur (S) fertility on the organosulphur compound content of garlic bulbs, especially alliin, as a specific flavour quality trait related to the health-value of garlic. Three cultivars, 'Printanor', 'Morasol' and 'Messidr6me', were grown in the greenhouse and in vitro. Sulphur was increased in the macronutrients within the range 0 - 144 mg l(-1). Numerous combinations of both S and N were tested in vitro. In the greenhouse, increasing N levels, from 184.8 mg l(-1) to 856.8 mg l(-1), were tested with a single high level of S (128 mg l(-1)). Increasing S alone did not affect the growth or bulb weight of any garlic cultivar, but slightly increased the alliin content of bulbs. No symptoms of S deficiency were observed under greenhouse conditions, although the same garlic cultivars grown in vitro at 0 mg S l(-1) clearly suffered, their size being greatly reduced. This suggests...
This chapter provides a review of the current status of androgenesis and doubled-haploid producti... more This chapter provides a review of the current status of androgenesis and doubled-haploid production in the different food legumes (including chickpea, field pea, lentil, soyabean, common bean and lupin) and outlines some strategies to overcome this recalcitrance.
... Document details. Title The Lathyrus paradox: a "poor man's diet" or a remarka... more ... Document details. Title The Lathyrus paradox: a "poor man's diet" or a remarkable genetic resource for protein legume breeding? Authors Ochatt, SJ; Abirached-Darmency, M.; Marget, P.; Aubert, G. Editors Ochatt, S.;Jain, SM ...
I am delighted to announce that Plant Cell Tissue and Organ Culture: Journal of Plant Biotechnolo... more I am delighted to announce that Plant Cell Tissue and Organ Culture: Journal of Plant Biotechnology (SpringerNature) will publish a Special Issue on Secondary Metabolites and Medicinal Plant Biotechnology. This Special Issue will include research on all aspects of the development of biotechnology-based approaches for the increased biomass production and better understanding of the production and synthesis of secondary metabolites by in vitro cell and tissue cultures of medicinal plants. One or two authoritative reviews, Original Research Articles, Research Notes and Protocol papers will be considered. Submissions are welcome up to October 31st 2021, and should be prepared in compliance with the journal guidelines. Publication is expected early in 2022. This Special Issue will be co-edited by (alphabetically) A.R. Alan (Turkey), A. Bhattacharya (India), C. Hano (France), K.V. Kiselev (Russia), P.L. Marconi (Argentina), W.C. Otoni (Brazil), S.Y. Park (Republic of Korea), K. Tang (China), P.J. Weathers (USA), and S. Ochatt (France) as editor-in-chief. KEYWORDS Agrobacterium, biochemical analyses, callus, cell suspensions, CRISPR/Cas9, elicitors, flow cytometry, fluxomics, gene expression, gene transfer, genetic stability, genome editing, genomics, HPLC, medicinal plants, metabolomics, microscopy, molecular analyses, organogenesis, proteomics, protoplasts, RAPD, somaclonal variation, somatic embryogenesis, specialized metabolites, transcriptomics
Uploads
Papers by Sergio Ochatt
India. This species is a promising natural source of bioactive compounds including galanthamine (GAL), an
anti-Alzheimer drug. In vitro regeneration in the Amaryllidaceae is often challenging. This study assessed the
use of meta-Topolin (mT) on in vitro regeneration of C. malabaricum. Shoot explants were cultured on Murashige and Skoog (MS) medium supplemented with 0.5, 2.5, 5.0, 7.5 and 10.0 mM mT for six weeks, whereby
7.5 mM mT resulted in the maximum multiplication of adventitious shoots, much higher than the control.
The biochemical accumulation of eleven different phenolic acids was quantified by UHPLC-MS/MS analysis,
and it appeared that mT-treated cultures exhibited the highest concentration of phenolic acids. In particular,
increased concentrations of gallic acid, protocatechuic acid, syringic acid, p-hydroxybenzoic acid, salicylic
acid and vanillic acid were detected compared to the control. mT (2.5 and 5.0 mM) produced the maximum
amount of chlorogenic acid, ferulic acid, p-coumaric acid and sinapic acid. However, an increased content of
caffeic acid was produced on PGR-free medium. These findings highlight the beneficial effect and validate
the rising importance of mT for in vitro regeneration studies. This study will serve as a potential protocol to
conserve and restore the medically important C. malabaricum.
pharmacological leads. Plant-derived biomolecules have historically proven their value as a source of therapeutic drugs and
hold an important potential for the identifcation and characterization of novel drug leads. Many diferent alkaloids possessing a broad range of pharmacological activities have been isolated from plants belonging to the Amaryllidaceae family.
Galanthamine (GAL) is a selective, reversible and an Amaryllidaceae-derived acetylcholinesterase inhibitor used for the
treatment of Alzheimer’s disease (AD) and other neurological diseases. Naturally, the bioavailability of Amaryllidaceae
alkaloids including GAL is low. Due to the signifcantly increased demand of GAL by the pharmaceutical industries and
the inadequate availability of natural resources, in vitro culture ofers an alternative approach for its sustainable production.
Thus, diferent biotechnological tools can optimize the in vitro GAL biosynthesis for treating AD, such as manipulation
of plant growth regulators, photoperiod, elicitors, and bioreactors systems, besides being an environmentally sustainable
approach, which protects the native biodiversity in a circular bioeconomy context. In the present review, we highlight the
biosynthesis of GAL by plant in vitro systems including its mode of action. This article should also provide a starting point
in the scaling-up of the biotechnological production of this valuable alkaloid.
India. This species is a promising natural source of bioactive compounds including galanthamine (GAL), an
anti-Alzheimer drug. In vitro regeneration in the Amaryllidaceae is often challenging. This study assessed the
use of meta-Topolin (mT) on in vitro regeneration of C. malabaricum. Shoot explants were cultured on Murashige and Skoog (MS) medium supplemented with 0.5, 2.5, 5.0, 7.5 and 10.0 mM mT for six weeks, whereby
7.5 mM mT resulted in the maximum multiplication of adventitious shoots, much higher than the control.
The biochemical accumulation of eleven different phenolic acids was quantified by UHPLC-MS/MS analysis,
and it appeared that mT-treated cultures exhibited the highest concentration of phenolic acids. In particular,
increased concentrations of gallic acid, protocatechuic acid, syringic acid, p-hydroxybenzoic acid, salicylic
acid and vanillic acid were detected compared to the control. mT (2.5 and 5.0 mM) produced the maximum
amount of chlorogenic acid, ferulic acid, p-coumaric acid and sinapic acid. However, an increased content of
caffeic acid was produced on PGR-free medium. These findings highlight the beneficial effect and validate
the rising importance of mT for in vitro regeneration studies. This study will serve as a potential protocol to
conserve and restore the medically important C. malabaricum.
pharmacological leads. Plant-derived biomolecules have historically proven their value as a source of therapeutic drugs and
hold an important potential for the identifcation and characterization of novel drug leads. Many diferent alkaloids possessing a broad range of pharmacological activities have been isolated from plants belonging to the Amaryllidaceae family.
Galanthamine (GAL) is a selective, reversible and an Amaryllidaceae-derived acetylcholinesterase inhibitor used for the
treatment of Alzheimer’s disease (AD) and other neurological diseases. Naturally, the bioavailability of Amaryllidaceae
alkaloids including GAL is low. Due to the signifcantly increased demand of GAL by the pharmaceutical industries and
the inadequate availability of natural resources, in vitro culture ofers an alternative approach for its sustainable production.
Thus, diferent biotechnological tools can optimize the in vitro GAL biosynthesis for treating AD, such as manipulation
of plant growth regulators, photoperiod, elicitors, and bioreactors systems, besides being an environmentally sustainable
approach, which protects the native biodiversity in a circular bioeconomy context. In the present review, we highlight the
biosynthesis of GAL by plant in vitro systems including its mode of action. This article should also provide a starting point
in the scaling-up of the biotechnological production of this valuable alkaloid.
This Special Issue will include research on all aspects of the development of biotechnology-based approaches for the increased biomass production and better understanding of the production and synthesis of secondary metabolites by in vitro cell and tissue cultures of medicinal plants. One or two authoritative reviews, Original Research Articles, Research Notes and Protocol papers will be considered. Submissions are welcome up to October 31st 2021, and should be prepared in compliance with the journal guidelines. Publication is expected early in 2022.
This Special Issue will be co-edited by (alphabetically) A.R. Alan (Turkey), A. Bhattacharya (India), C. Hano (France), K.V. Kiselev (Russia), P.L. Marconi (Argentina), W.C. Otoni (Brazil), S.Y. Park (Republic of Korea), K. Tang (China), P.J. Weathers (USA), and S. Ochatt (France) as editor-in-chief.
KEYWORDS
Agrobacterium, biochemical analyses, callus, cell suspensions, CRISPR/Cas9, elicitors, flow cytometry, fluxomics, gene expression, gene transfer, genetic stability, genome editing, genomics, HPLC, medicinal plants, metabolomics, microscopy, molecular analyses, organogenesis, proteomics, protoplasts, RAPD, somaclonal variation, somatic embryogenesis, specialized metabolites, transcriptomics