Papers by Selma Gökahmetoğlu
Sağlık bilimleri dergisi, Jun 1, 2013
Chlamydia trachomatis, tüm dünyada en yaygın seksüel geçişli bakteriyel enfeksiyon ajanı olarak b... more Chlamydia trachomatis, tüm dünyada en yaygın seksüel geçişli bakteriyel enfeksiyon ajanı olarak bilinir. Bu mikroorganizma zorunlu hücre içi parazitidir. C.trachomatis, trahom gibi göz enfeksiyonlarına, yenidoğanlarda pnömonilere, genitoüriner sistem enfeksiyonlarına ve Lenfogranüloma venerum (LGV) suşları tarafından oluşturulan ve özellikle genital bölgedeki lenf düğümlerinde patoloji ile karakterize LGV'a neden olmaktadır. Bu çalışmada klinik örneklerde Polimeraz Zincir Reaksiyonu (PCR) ve Gerçek Zamanlı PCR ile C. trachomatis'in araştırılması amaçlandı. Şubat 2010-Mart 2010 tarihleri arasında, Erciyes Üniversitesi Gevher Nesibe Araştırma ve Uygulama Hastanesi Kadın Hastalıkları ve Doğum poliklinikleri ile Üroloji polikliniklerine genital akıntı ön tanısıyla başvuran ve genital enfeksiyon düşünülen 50 hastadan alınan sürüntü örnekleri çalışmaya dahil edildi. Çalışmaya alınan 50 sürüntü örneğinin 1'inde (%2) her iki yöntem ile C. trachomatis-DNA pozitif bulundu. Sonuç olarak C. trachomatis DNA araştırılmasında iki PCR yöntemi arasında fark bulunamadı.
DergiPark (Istanbul University), Aug 1, 2018
GİRİŞ ve AMAÇ: Akut bronşiolit, viral etkenlerle oluşan ve çocuklarda sık görülen bir alt solunum... more GİRİŞ ve AMAÇ: Akut bronşiolit, viral etkenlerle oluşan ve çocuklarda sık görülen bir alt solunum yolu hastalığıdır. Bölgemizde akut bronşiolit tanısı alan çocuklarda viral etkenlerin ve özellikle Human Metapnömovirüs sıklığının belirlenmesi, olguların klinik, laboratuvar ve radyolojik bulgularının irdelenmesi amaçlandı.
Journal of Basic and Clinical Health Sciences
Background and Purpose: Cytomegalovirus causes asymptomatic disease in individuals with normal im... more Background and Purpose: Cytomegalovirus causes asymptomatic disease in individuals with normal immune system; and leads to serious complications in immunocompromised individuals and fetus. In CMV, gB is the most studied glycoprotein in terms of genotyping. Up to now, four different gB genotypes (gB 1-4) of CMV have been identified. In this study, it was aimed to determine the genotypes of CMV strains obtained from patients with immune deficiency. Methods: Twenty children and 29 adults, 49 patients who were followed in the Department of Adult Hematology and Pediatric Hematology were included in the study. DNA isolation was performed from samples with CMV DNA level of 1000 IU / ml and above, and 474 bp region from the gB region of the virus was amplified by nested PCR. This region was sequenced by the Sanger (ABI 3500 Prism) sequencing. Next generation sequencing (NGS) method was applied to the samples that CMV genotype could not be determined by Sanger sequencing. Results: Distributi...
Flora infeksiyon hastalıkları ve klinik mikrobiyoloji dergisi, 2004
Bozok Tıp Dergisi, 2021
Objective: Recently, CMV, EBV, BKV and JC virus have been suggested to contribute to gliomagenesi... more Objective: Recently, CMV, EBV, BKV and JC virus have been suggested to contribute to gliomagenesis, but evidence is largely contradictory. The aim of this study was to test 50 tissue samples from patients with GBM and 21 normal brain tissues obtained from autopsy material of patients without brain tumors in order to investigate the presence of possible oncogenic microorganisms, including EBV, JCV, BKV and Toxoplasma gondii, and to evaluate patient clinical characteristics of patients with respect to microorganism findings. Material and Method: Fifty formalin-fixed paraffin-embedded specimens obtained from glioblastoma tissue and 21 normal brain tissues obtained in the autopsy of individuals without brain tumor were retrospectively analyzed. After de-paraffinization of tissue samples, DNA extraction was performed for real-time polymerase chain reaction (RT-PCR) analysis to detect BKV, JCV, EBV and Toxoplasma gondii via commercially-available multiplex kits. Results: Strikingly, viral...
Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi, 2013
Health care workers are at occupational risk for many infectious diseases during patient care. Ma... more Health care workers are at occupational risk for many infectious diseases during patient care. Maintenance of immunity against measles, mumps, rubella, varicella, and hepatitis B is therefore an essential part in their prevention. The aim of this study was to describe health care workers immunity to these infections and determine the factors of age, occupation, position of employment, and length of employment. A total of 964 HCW participated in this study. Immunity against hepatitis B was significantly higher among nurses (p< 0.05). This situation was associatied with better awareness among nurses.
Journal of Pediatric Infectious Diseases, 2021
Objective The most common childhood convulsive disorder happens to be febrile seizure (FS), which... more Objective The most common childhood convulsive disorder happens to be febrile seizure (FS), which is an important health problem leading to economic burden and parental anxiety. Further investigation into the etiological causes of FS will guide us for appropriate measures during the follow-up period. The aim of study was to identify the percentage of viral and bacterial pathogens in the etiological causes of children with FS, and also if there is any difference between simple and complex FSs.Methods This prospective study randomly enrolled 100 pediatric patients with FS between January 2017 and July 2017. Nasopharyngeal swabs were obtained from all children at presentation. The respiratory panel was performed with a multiplex real-time polymerase chain reaction method to detect the 21 most common viruses. A complete blood count, absolute neutrophil count, absolute lymphocyte count, C-reactive protein, erythrocyte sedimentation rate, procalcitonin, blood culture, throat culture, urin...
Turkish Journal of Medical Sciences, 2005
This study was planned in order to make good the lack of information about population-based data ... more This study was planned in order to make good the lack of information about population-based data on the prevalence of brucellosis in the Kayseri region. This cross-sectional research was performed on 1850 individuals living in the rural regionaround Kayseri. People aged 15-85 in 9 districts in this rural areas were interviewed and blood samples were screened using the Rose Bengal Agglutination test. The overall prevalence of brucellosis was 3.4%. Although the rate was higher in females (3.7%) than in males (2.9%), there was no significant difference. The lowest prevalence (2.0%) was observed in the 25-34 age group, and the highest prevalence (4.3% and 4.1%) were in the 35-44 and 15-24 age groups, respectively. The highest prevalence was observed in the illiterate group. The prevalence was much higher among people in close contact with sheep and/or cattle, those making home made cheese, butter and cream and also in those who consumed butter and cream. It was concluded that although t...
Journal of Hepatology, 2019
assessed in human and mice models of non-alcoholic fatty liver diseases (NAFLD). Results: Insulin... more assessed in human and mice models of non-alcoholic fatty liver diseases (NAFLD). Results: Insulin-stimulated glucose uptake was reduced in adipocytes treated with sera from HCV-infected patients as compared to non-infected controls. Overexpression of the HCV-3a core protein in the mouse liver led to the development of steatosis. Insulin sensitivity of mice was reduced, as shown by ITT and by decreased insulininduced Akt-S473 phosphorylation in both liver and skeletal muscle. In mice livers, the expression of some hepatokines, previously found to be altered in treated patients of our cohort (i.e. Fetuin-A, Igfbp7, Angptl6 and Rbp4), positively correlated with the level of HCV core expression. Moreover, Igfbp7 mRNA expression was increased also in livers of high-sucrose, high-fat diet mice. In a human transcriptome meta-analysis of NAFLD patients, IGFBP7 upregulation was associated with an advanced severity of the disease. Conclusion: Taken together, these data indicate that HCV infection affects the hepatokine secretion profile to induce peripheral insulin resistance, providing a model of hepatogenous diabetes. SAT-167 Hepatitis-E-genome sequencing via capture-probe targeting and NGS allows characterisation of viral variability
Clinical Laboratory, 2019
BACKGROUND HCV virus infections are one of the major health problems in the world that can cause ... more BACKGROUND HCV virus infections are one of the major health problems in the world that can cause cirrhosis and liver cancer at a higher rate than other hepatitis data. The aim of this study was to determine the prevalence of mixed infections with different HCV genotypes in Turkey and also to evaluate the current HCV genotype and sub-type distributions by a multicentered assessment. METHODS The HCV genotype data of 17,578 hepatitis C patients collected from 23 centers from different geographic regions covering all Turkey were collected. The data included information about the HCV genotypes in the last 10 years (between 2007 and 2016), demographic properties of the patients and the methods/systems used to determine the genotypes. RESULTS Two hundred twenty-eight of the patients (1.3%) had mixed genotype. The most common mixed genotype combination was 1b + 4 (0.83%) followed by 1a + 1b (0.26%). Genotype distribution varies according to geographical regions. However, genotype 1 (82.92%) was the most common genotype in all regions and all years. This was followed by genotype 3 (7.07%) and genotype 4 (5.43%). A variety of methods were used by the centers including sequencing, pyrosequencing, real-time PCR, in-house RFLP, reverse hybridization (LIPA), and hybridization. CONCLUSIONS Infection with mixed HCV genotypes in Turkey is uncommon. Genotype distribution varies according to geographic regions; the most common genotype 1 is encountered all over the country, while genotypes 3 and 4 are only in some of the centers. Since there is limited information about mixed HCV infection, further investigations are needed to determine the clinical importance of mixed HCV infection.
Journal of Immunology and Clinical Microbiology, 2017
Prevalence rates of 10% to 24% have been reported in some areas (4). The most frequently observed... more Prevalence rates of 10% to 24% have been reported in some areas (4). The most frequently observed HCV genotype in our country is G1b, with a rate of 68-94%, but in recent years there has been an increase in G4 infections (5-8). With a 48-week administration of the combination therapy of pegylated alpha and ribavirin (PegIFN/RBV), the sustained virological response (SVR)
Mikrobiyoloji Bulteni, 2016
Yoğun bakım üniteleri (YBÜ)'nde bir haftadan daha uzun süre yatan hastalarda Candida türleri ile ... more Yoğun bakım üniteleri (YBÜ)'nde bir haftadan daha uzun süre yatan hastalarda Candida türleri ile kolonizasyon oranı %80'in üzerine çıkmakta ve kolonize hastaların ortalama %10'unda invazif hastalık gelişmektedir. Yoğun bakım hastalarında invazif kandidiyaz (İK), septik şok ve yüksek mortalite ile seyrettiğinden hızlı tanı ve tedavinin önemi büyüktür. Bu çalışmada, İK gelişme riski yüksek olan ve erken antifungal tedaviden fayda görecek olan yoğun bakım hastalarında, Candida kolonizasyon indeksi (CI) ve Candida skoru (CS) belirlenerek; invazif enfeksiyon ile CI ve CS arasındaki ilişkinin değerlendirilmesi amaçlanmıştır. Çalışmaya, Nisan 2014-Temmuz 2015 tarihleri arasında Kayseri Eğitim ve Araştırma Hastanesi, Anestezi Bölümü Yoğun Bakım Ünitesinde yedi gün ve daha uzun süre yatmış olan 80 hasta (34 kadın, 46 erkek; yaş aralığı: 12-92 yıl, yaş ortalaması: 69.57 ± 16.30) dahil edilmiştir. Hastaların hiçbirinde nötropeni mevcut değildir. YBÜ'ne yatıştan sonra her hastadan, 0. gün ve daha sonra da her
Journal of Clinical Laboratory Analysis, 2016
Background: In this study, the fresh stool samples from 254 children under 5 years of age with ac... more Background: In this study, the fresh stool samples from 254 children under 5 years of age with acute gastroenteritis which were delivered between October 2012 and December 2013 were collected. Methods: In the stool samples, rotavirus antigens were investigated using two different immunochromatographic methods which are routinely used at different times, namely the RIDA â QUICK Rotavirus/Adenovirus Combi Test (R-Biopharm AG, Germany) and the Genx â Rotavirus Test (Diamed-Lab, Turkey), in addition to the Rotavirus Ag (Stool) ELISA (DRG, Germany) kit. The results were compared with reverse transcriptase PCR (RT-PCR). Results: When the Genx â Rotavirus Test and RIDA â QUICK Rotavirus/Adenovirus Combi Test immunochromatographic methods were compared with RT-PCR, their sensitivity and specificity were found as 97.1%, 100%, and 80.4%, 72%, respectively. As to the Rotavirus Ag (Stool) ELISA method, on the other hand, its sensitivity was found to be 95.1% and its specificity was 86.5%. The most common genotype was G9P[8] (40%), which was followed by the G1P[8] (18.7%) and G3P[8] (9.6%) genotypes. Conclusion: Consequently, it was revealed that the sensitivity of ELISA and immunochromatographic methods, which provide results in a short time and are used in the investigation of rotavirus antigen, was high and their specificity was low; further studies to determine the distribution of G and P genotypes will contribute to establishing strategies for vaccine development for rotavirus in the world.
Biotechnology & Biotechnological Equipment, 2015
ABSTRACT Hepatitis C infection is a public health problem. The aim of this retrospective study wa... more ABSTRACT Hepatitis C infection is a public health problem. The aim of this retrospective study was to determine the distribution of hepatitis C virus (HCV) genotypes in seven regions of Turkey, by evaluating 7002 patients with chronic HCV in a six-year period. During the 2009–2014 period, serum/plasma samples from 7002 new consecutive HCV RNA positive patients were collected. The female patients were 3867 (55.2%). The genotype distribution of HCV patiens was evaluated by ages and years. Statistical analysis was performed by using the Mann–Whitney test and the χ2 analysis. During the six-year period, genotype 1b was the most common genotype (67.7%) followed by untypeable genotype 1 (7.7%), genotype 4 (7.3%) and genotype 3 (6.7%). In 2014, genotype 3 was the second most common one (11.3%) and genotype 4 was the third most common one (9.8%). In the group with <25 years old patients, genotype 1b was most common (78.48%, 62/79) between the years of 2009 and 2011, whereas genotype 3 (34.8%, 86/247), between the years of 2012 and 2014. Genotype 1b was the most common in the groups between 26 and 35 years, 36 and 45 years, 46 and 55 years, 56 and 65 years. The rate of genotype 3 was increased from 4.78% to 10.06% and the rate of genotype 4 was increased from 1.3% to 3.84%, from 2009–2011 to 2012–2014. In recent years, genotypes 3 and 4 have gained importance. New therapeutic strategies and survey studies may be required for the modified HCV genotype pattern.
Erciyes Tıp Dergisi/Erciyes Medical Journal, 2015
Objective: The etiology of Attention-Deficit Hyperactivite Disorder (ADHD) is still unclear. In t... more Objective: The etiology of Attention-Deficit Hyperactivite Disorder (ADHD) is still unclear. In the present study, we aimed to demonstrate the relationship among anti-ganglioside antibodies, anti-glutamate receptor antibodies, and anti-glutamic acit decarboksilaz (anti-GAD) antibodies, which are believed to be involved in the etiology of ADHD. Materials and Methods: The study included 36 children who were diagnosed with ADHD according to DSM IV diagnostic criteria and 21 healthy children as the control group. In all subjects, anti-ganglioside antibodies, anti-glutamate receptor antibodies, and anti-GAD antibodies were studied in the Microbiology Laboratory of Erciyes University, Medical School. Results: The mean age was 9.34 years in the ADHD group, which consisted of 5 girls and 31 boys. The mean age was 7.8 years in the control group, which consisted of 8 girls and 13 boys. No significant differences were observed in the levels of antiganglioside antibodies, anti-glutamate receptor antibodies, and anti-GAD antibodies between the ADHD and control groups. Conclusion: Although the etiology is unknown in ADHD, it is believed that autoimmune factors may be involved in the etiopathogenesis according to currently available information. However, there is a need for further studies with larger sample size to clarify the linkage between ADHD and anti-neuronal antibody levels.
European journal of gynaecological oncology, 2003
To investigate the frequency of human papillomavirus (HPV) infection among low-risk women for cer... more To investigate the frequency of human papillomavirus (HPV) infection among low-risk women for cervical cancer in our region. In one year period, 230 consecutive women at low risk of developing cervical cancer were enrolled to the study. HPV DNA testing was performed by Hybrid Capture-I System (HC-I) and groups were constituted by HPV-positive and HPV-negative women. A comparison of the groups according to age, obstetric history and age at the beginning of sexual intercourse was made. Statistical analysis was performed. The frequency rate of HPV infection was demonstrated to be 6.1% (n = 14) in our study (5.9% in women < or = 45 years and 7.7% in women > 45 years). Age-dependent differences were not observed between groups. There was no significant difference between HPV-positive and negative women regarding obstetric characteristics and mean age at first intercourse. This study provided significant information on the frequency of HPV infection of low-risk women in our region. ...
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Papers by Selma Gökahmetoğlu