Black Slavonian pig is an indigenous pig breed black in colour, resistant and convenient for keep... more Black Slavonian pig is an indigenous pig breed black in colour, resistant and convenient for keeping in extensive conditions. It is also characterized by good meat quality, suitable for typical traditional meat products. Traditionally produced food came into the focus which led to more often use of geographical indications as the valuable rural development strategy. Since Black Slavonian pig is a late maturing breed with low lean meat content, meat processors often use the meat of modern pig breeds in the production of traditional products. Molecular identification of breed is a good tool for authentication of meat. Since age and sex are among the most significant sources of variation of the carcass and meat quality traits in pigs, the research on the optimal time for slaughter with respect to these factors could increase the profitability of pork production. The genetic influence on meat quality traits is nowadays well described by the use of molecular markers and candidate genes s...
Aim To investigate the association of nephrolithiasis and solute carrier family 2, facilitated gl... more Aim To investigate the association of nephrolithiasis and solute carrier family 2, facilitated glucose transporter, member 9 (SLC2A9), also known as glucose transporter type 9, Glut9. Methods A total of 145 participants were recruited in the period April-October 2008 from the Department of Mineral Research of the Medical School Osijek, Osijek, Croatia; 58 (40%) had confirmed nephrolithiasis and 87 (60%) were asymptomatic. Four single nucleotide polymorphisms (SNP) from the SLC2A9 gene were genotyped in both groups (rs733175, rs6449213, rs1014290, and rs737267). Results There was a weak but significant association of all 4 SNPs and nephrolithiasis (P = 0.029 for rs733175; P = 0.006 for rs6449213; P = 0.020 for rs1014290, and P = 0.011 for rs737267). Logistic regression in an age-and sex-adjusted model suggested that genotype C/T for rs6449213 had odds ratio for nephrolithiasis of 2.89 (95% confidence interval 1.13-7.40). This SNP explained a total of 4.4% of nephrolithiasis variance. Conclusion Development of nephrolithiasis may be associated with SLC2A9 gene. Further studies are needed to clarify the role of SLC2A9 gene as a link between uric acid and nephrolithiasis.
The purpose of this study was to determine prevalence of Chlamydia trachomatis (Ct) urogenital in... more The purpose of this study was to determine prevalence of Chlamydia trachomatis (Ct) urogenital infection and its serotype distribution from clinical samples in northeastern Croatia. During a 3-year period, 2,379 urogenital samples were analyzed by real-time polymerase chain reaction (A group), while 4,846 genital swabs were analyzed by direct fluorescent antibody test (B group). 132 Ct positive specimens were genotyped by omp1 gene sequencing. The prevalence rate of Ct was 3.2 % in A and 1 % in B group. The most prevalent chlamydial genotype was E (44 %), followed by F (33 %), K (11.5 %), G (8 %), J/UW (5.3 %), D-IC (4.4 %), D-B120 (1.8 %), and B/IU, J/IU, Ia/IU (0.9 % each) serotypes. Single-nucleotide polymorphisms (SNPs) of omp1 gene were detected in E, K, and G serotypes.
International Journal of Infectious Diseases, 2010
Background: HIV and syphilis affect similar patient groups and co-infection is common. In HIV inf... more Background: HIV and syphilis affect similar patient groups and co-infection is common. In HIV infected patients, clinical manifestation of syphilis may be atypical and different from HIV uninfected cases. This study aimed to compare the characteristics of syphilis between HIVseropositive and HIVseronegative patients in Thai Medical School Hospital. Methods: Retrospectively chart review of new cases of syphilis who attended the STD clinic, Chulalongkorn Hospital, Bangkok,Thailand from 1st June 2001 to 31st May 2006 was conducted. Results: There were totally 385 new cases of syphilis during the studied period and HIV screening was done in 325 patients.The prevalence of HIV infection among syphilis patients was 33.2%. We found that younger age, male gender, and men who have sex with men (MSM) were significantly associated with HIV infection (p<0.05). Secondary syphilis was more common manifestation in HIV infected individuals (p< 0.005). Syphilis among HIV infected patients had significantly lower VDRL titer (p<0.005) and 15.9% had nonreactive VDRL. Conclusion: HIV co-infection should be considered in syphilitic clients particularly with younger age, being male gender, MSM, and whose clinical manifestations are secondary syphilis. Non-reactive or low titer VDRL does not exclude syphilis in HIV infected patients and treponemal antibody test is needed for screening and diagnosis.
This is a first cross-sectional study on the prevalence and distribution of HPV infection in asym... more This is a first cross-sectional study on the prevalence and distribution of HPV infection in asymptomatic, heterosexual men from Osijek-Baranja County, Croatia. Between 2009 and 2011, 330 men tested for sexually transmitted diseases (STDs) were recruited. Their genital swabs were tested for high-risk HPV (HR HPV) infection by the AMPLICOR HPV test and further genotyped by the Linear Array HPV Genotyping Test (both by Roche). Infection with a single HR HPV was detected in almost one third of men (39%) whereas multiple HPV types, in more than a half of HR HPV-positive men (61%). The highest HR HPV prevalence was detected in those younger than 20 (37.5%) and lowest in 31-35 year old men (27.8%). The most common genotypes were HPV 6 (24%), 16 (17.8%), 51 (9%), 52 (6%), 35, 55, 66, 84 (each 5%), 31, 62 (each 4%), 39, 58, 59, 83 (each 2.5%), and finally 56, 18, 53, and 54 (each 1.3%). Having more than one sexual partner per year was significantly associated with HR HPV infection in age gr...
This is a first cross-sectional study on the prevalence and distribution of HPV infection in asym... more This is a first cross-sectional study on the prevalence and distribution of HPV infection in asymptomatic, heterosexual men from Osijek-Baranja County, Croatia. Between 2009 and 2011, 330 men tested for sexually transmitted diseases (STDs) were recruited. Their genital swabs were tested for high-risk HPV (HR HPV) infection by the AMPLICOR HPV test and further genotyped by the Linear Array HPV Genotyping Test (both by Roche). Infection with a single HR HPV was detected in almost one third of men (39%) whereas multiple HPV types, in more than a half of HR HPV-positive men (61%). The highest HR HPV prevalence was detected in those younger than 20 (37.5%) and lowest in 31-35 year old men (27.8%). The most common genotypes were HPV 6 (24%), 16 (17.8%), 51 (9%), 52 (6%), 35, 55, 66, 84 (each 5%), 31, 62 (each 4%), 39, 58, 59, 83 (each 2.5%), and finally 56, 18, 53, and 54 (each 1.3%). Having more than one sexual partner per year was significantly associated with HR HPV infection in age gr...
The aims of this study were to determine the HCV-RNA viral load, genotype distribution, risk fact... more The aims of this study were to determine the HCV-RNA viral load, genotype distribution, risk factors and symptoms of HCVRNA positive viral load in HCV antibody-positive patients from north-eastern Croatia. From January 2009 to December 2011, 203 HCV antibody- positive patients (130 men and 73 women; median age 44.5 years) were analyzed for HCV-RNA by the COBAS TaqMan HCV test and genotyped by the Linear Array HCV Genotyping test (both from Roche). All patients completed a structured questionnaire about risk factors and symptoms. The HCV-RNA percentage was 61.1% and was similar for men and women. The HCV-RNA viral load increased with age: while 55% of 20-50 year old patients were HCV-RNA positive, 73% of patients >50 years were positive (p=0.021). Genotype 1 was the most prevalent genotype (79.8%), followed by 3 (12.9%), 4 (6.5%), and 2 (0.8%); genotypes 5 and 6 were not determined. Patients with genotype 1 (median, 50 years) were older than patients with 3 (median, 33.5 years) or...
To analyze statistically and logically the significance of genetic matches between skeletal remai... more To analyze statistically and logically the significance of genetic matches between skeletal remains and relatives of missing persons in the process of identification of war victims by DNA typing. DNA was isolated from bone and blood samples and short tandem repeat (STR) loci were typed by using AmpFLSTR Profiler, Profiler Plus, and Identifiler kits. Novel mini-haplotype analysis that compares matches in all three-locus combinations of alleles was developed and used in the analysis of inbreeding in the group of 295 unrelated individuals. While comparing 98 skeletal remains exhumed in the process of identification of war victims in Croatia with over 3,000 genotypes of relatives of missing persons, we revealed 20 cases of 14-locus matches and 4 cases of 15-locus matches between unrelated people. We hypothesized that this unexpectedly high number of false matches might be a consequence of local inbreeding and supported this hypothesis with very low correlation between the probability of...
The purpose of this study was to determine prevalence of Chlamydia trachomatis (Ct) urogenital in... more The purpose of this study was to determine prevalence of Chlamydia trachomatis (Ct) urogenital infection and its serotype distribution from clinical samples in north-eastern Croatia. During a 3-year period, 2,379 urogenital samples were analyzed by real-time polymerase chain reaction (A group), while 4,846 genital swabs were analyzed by direct fluorescent antibody test (B group). 132 Ct positive specimens were genotyped by omp1 gene sequencing. The prevalence rate of Ct was 3.2 % in A and 1 % in B group. The most prevalent chlamydial genotype was E (44 %), followed by F (33 %), K (11.5 %), G (8 %), J/UW (5.3 %), D-IC (4.4 %), D-B120 (1.8 %), and B/IU, J/IU, Ia/IU (0.9 % each) serotypes. Single-nucleotide polymorphisms (SNPs) of omp1 gene were detected in E, K, and G serotypes. Some of these SNPs (C/T at position 272 and G/A at position 813 in E strain; C/T at position 884 in D strain) might represent novel omp1 variants.
To investigate the association of nephrolithiasis and solute carrier family 2, facilitated glucos... more To investigate the association of nephrolithiasis and solute carrier family 2, facilitated glucose transporter, member 9 (SLC2A9), also known as glucose transporter type 9, Glut9.
Aiming to evaluate the effects of population substructure on the reliability of a DNA corresponde... more Aiming to evaluate the effects of population substructure on the reliability of a DNA correspondence in the process of human identification, we used the model of ''in silico'' constructed populations with and without substructure. Effects of population substructure were evaluated at the level of locus heterozygosity, Hardy-Weinberg equilibrium and mini-haplotype distribution. Inbreeding in a subpopulation of 100 individuals through 10 generations did not significantly alter the level of heterozygosity and Hardy-Weinberg equilibrium. However, analysis of mini-haplotype distribution revealed a significant homogenization in separated subpopulations. Average observed mini-haplotype frequency (f o ) increased to threefold from expected values (f e ), and the number of mini-haplotypes with f o ⁄ f e above 10 increased over sixfold, suggesting that the effects of population substructure on calculated likelihood ratios (LR) might be larger than previously estimated. In most criminal cases, this would not represent a problem, whereas for identifications in large-scale mass fatality events, population substructure might considerably increase the risk of false identification.
International Journal of Infectious Diseases, 2008
Background: Sexually transmitted infections (STIs) constitute a significant public health concern... more Background: Sexually transmitted infections (STIs) constitute a significant public health concern, especially in resource poor settings. Notification and medical evaluation of recent sexual partners of infected patients is important for STI control. However, research on partner notification effectiveness in resource poor settings is very limited.
The result of empirical testing of forensic DNA match probabilities for Croatia is reported. It i... more The result of empirical testing of forensic DNA match probabilities for Croatia is reported. It is concluded that if consideration is given to relatedness and subpopulation effects the model of Balding and Nichols appears to give very good predictions.
The purpose of this study was to determine prevalence of Chlamydia trachomatis (Ct) urogenital in... more The purpose of this study was to determine prevalence of Chlamydia trachomatis (Ct) urogenital infection and its serotype distribution from clinical samples in north-eastern Croatia. During a 3-year period, 2,379 urogenital samples were analyzed by real-time polymerase chain reaction (A group), while 4,846 genital swabs were analyzed by direct fluorescent antibody test (B group). 132 Ct positive specimens were genotyped by omp1 gene sequencing. The prevalence rate of Ct was 3.2 % in A and 1 % in B group. The most prevalent chlamydial genotype was E (44 %), followed by F (33 %), K (11.5 %), G (8 %), J/UW (5.3 %), D-IC (4.4 %), D-B120 (1.8 %), and B/IU, J/IU, Ia/IU (0.9 % each) serotypes. Single-nucleotide polymorphisms (SNPs) of omp1 gene were detected in E, K, and G serotypes. Some of these SNPs (C/T at position 272 and G/A at position 813 in E strain; C/T at position 884 in D strain) might represent novel omp1 variants.
Black Slavonian pig is an indigenous pig breed black in colour, resistant and convenient for keep... more Black Slavonian pig is an indigenous pig breed black in colour, resistant and convenient for keeping in extensive conditions. It is also characterized by good meat quality, suitable for typical traditional meat products. Traditionally produced food came into the focus which led to more often use of geographical indications as the valuable rural development strategy. Since Black Slavonian pig is a late maturing breed with low lean meat content, meat processors often use the meat of modern pig breeds in the production of traditional products. Molecular identification of breed is a good tool for authentication of meat. Since age and sex are among the most significant sources of variation of the carcass and meat quality traits in pigs, the research on the optimal time for slaughter with respect to these factors could increase the profitability of pork production. The genetic influence on meat quality traits is nowadays well described by the use of molecular markers and candidate genes s...
Aim To investigate the association of nephrolithiasis and solute carrier family 2, facilitated gl... more Aim To investigate the association of nephrolithiasis and solute carrier family 2, facilitated glucose transporter, member 9 (SLC2A9), also known as glucose transporter type 9, Glut9. Methods A total of 145 participants were recruited in the period April-October 2008 from the Department of Mineral Research of the Medical School Osijek, Osijek, Croatia; 58 (40%) had confirmed nephrolithiasis and 87 (60%) were asymptomatic. Four single nucleotide polymorphisms (SNP) from the SLC2A9 gene were genotyped in both groups (rs733175, rs6449213, rs1014290, and rs737267). Results There was a weak but significant association of all 4 SNPs and nephrolithiasis (P = 0.029 for rs733175; P = 0.006 for rs6449213; P = 0.020 for rs1014290, and P = 0.011 for rs737267). Logistic regression in an age-and sex-adjusted model suggested that genotype C/T for rs6449213 had odds ratio for nephrolithiasis of 2.89 (95% confidence interval 1.13-7.40). This SNP explained a total of 4.4% of nephrolithiasis variance. Conclusion Development of nephrolithiasis may be associated with SLC2A9 gene. Further studies are needed to clarify the role of SLC2A9 gene as a link between uric acid and nephrolithiasis.
The purpose of this study was to determine prevalence of Chlamydia trachomatis (Ct) urogenital in... more The purpose of this study was to determine prevalence of Chlamydia trachomatis (Ct) urogenital infection and its serotype distribution from clinical samples in northeastern Croatia. During a 3-year period, 2,379 urogenital samples were analyzed by real-time polymerase chain reaction (A group), while 4,846 genital swabs were analyzed by direct fluorescent antibody test (B group). 132 Ct positive specimens were genotyped by omp1 gene sequencing. The prevalence rate of Ct was 3.2 % in A and 1 % in B group. The most prevalent chlamydial genotype was E (44 %), followed by F (33 %), K (11.5 %), G (8 %), J/UW (5.3 %), D-IC (4.4 %), D-B120 (1.8 %), and B/IU, J/IU, Ia/IU (0.9 % each) serotypes. Single-nucleotide polymorphisms (SNPs) of omp1 gene were detected in E, K, and G serotypes.
International Journal of Infectious Diseases, 2010
Background: HIV and syphilis affect similar patient groups and co-infection is common. In HIV inf... more Background: HIV and syphilis affect similar patient groups and co-infection is common. In HIV infected patients, clinical manifestation of syphilis may be atypical and different from HIV uninfected cases. This study aimed to compare the characteristics of syphilis between HIVseropositive and HIVseronegative patients in Thai Medical School Hospital. Methods: Retrospectively chart review of new cases of syphilis who attended the STD clinic, Chulalongkorn Hospital, Bangkok,Thailand from 1st June 2001 to 31st May 2006 was conducted. Results: There were totally 385 new cases of syphilis during the studied period and HIV screening was done in 325 patients.The prevalence of HIV infection among syphilis patients was 33.2%. We found that younger age, male gender, and men who have sex with men (MSM) were significantly associated with HIV infection (p<0.05). Secondary syphilis was more common manifestation in HIV infected individuals (p< 0.005). Syphilis among HIV infected patients had significantly lower VDRL titer (p<0.005) and 15.9% had nonreactive VDRL. Conclusion: HIV co-infection should be considered in syphilitic clients particularly with younger age, being male gender, MSM, and whose clinical manifestations are secondary syphilis. Non-reactive or low titer VDRL does not exclude syphilis in HIV infected patients and treponemal antibody test is needed for screening and diagnosis.
This is a first cross-sectional study on the prevalence and distribution of HPV infection in asym... more This is a first cross-sectional study on the prevalence and distribution of HPV infection in asymptomatic, heterosexual men from Osijek-Baranja County, Croatia. Between 2009 and 2011, 330 men tested for sexually transmitted diseases (STDs) were recruited. Their genital swabs were tested for high-risk HPV (HR HPV) infection by the AMPLICOR HPV test and further genotyped by the Linear Array HPV Genotyping Test (both by Roche). Infection with a single HR HPV was detected in almost one third of men (39%) whereas multiple HPV types, in more than a half of HR HPV-positive men (61%). The highest HR HPV prevalence was detected in those younger than 20 (37.5%) and lowest in 31-35 year old men (27.8%). The most common genotypes were HPV 6 (24%), 16 (17.8%), 51 (9%), 52 (6%), 35, 55, 66, 84 (each 5%), 31, 62 (each 4%), 39, 58, 59, 83 (each 2.5%), and finally 56, 18, 53, and 54 (each 1.3%). Having more than one sexual partner per year was significantly associated with HR HPV infection in age gr...
This is a first cross-sectional study on the prevalence and distribution of HPV infection in asym... more This is a first cross-sectional study on the prevalence and distribution of HPV infection in asymptomatic, heterosexual men from Osijek-Baranja County, Croatia. Between 2009 and 2011, 330 men tested for sexually transmitted diseases (STDs) were recruited. Their genital swabs were tested for high-risk HPV (HR HPV) infection by the AMPLICOR HPV test and further genotyped by the Linear Array HPV Genotyping Test (both by Roche). Infection with a single HR HPV was detected in almost one third of men (39%) whereas multiple HPV types, in more than a half of HR HPV-positive men (61%). The highest HR HPV prevalence was detected in those younger than 20 (37.5%) and lowest in 31-35 year old men (27.8%). The most common genotypes were HPV 6 (24%), 16 (17.8%), 51 (9%), 52 (6%), 35, 55, 66, 84 (each 5%), 31, 62 (each 4%), 39, 58, 59, 83 (each 2.5%), and finally 56, 18, 53, and 54 (each 1.3%). Having more than one sexual partner per year was significantly associated with HR HPV infection in age gr...
The aims of this study were to determine the HCV-RNA viral load, genotype distribution, risk fact... more The aims of this study were to determine the HCV-RNA viral load, genotype distribution, risk factors and symptoms of HCVRNA positive viral load in HCV antibody-positive patients from north-eastern Croatia. From January 2009 to December 2011, 203 HCV antibody- positive patients (130 men and 73 women; median age 44.5 years) were analyzed for HCV-RNA by the COBAS TaqMan HCV test and genotyped by the Linear Array HCV Genotyping test (both from Roche). All patients completed a structured questionnaire about risk factors and symptoms. The HCV-RNA percentage was 61.1% and was similar for men and women. The HCV-RNA viral load increased with age: while 55% of 20-50 year old patients were HCV-RNA positive, 73% of patients >50 years were positive (p=0.021). Genotype 1 was the most prevalent genotype (79.8%), followed by 3 (12.9%), 4 (6.5%), and 2 (0.8%); genotypes 5 and 6 were not determined. Patients with genotype 1 (median, 50 years) were older than patients with 3 (median, 33.5 years) or...
To analyze statistically and logically the significance of genetic matches between skeletal remai... more To analyze statistically and logically the significance of genetic matches between skeletal remains and relatives of missing persons in the process of identification of war victims by DNA typing. DNA was isolated from bone and blood samples and short tandem repeat (STR) loci were typed by using AmpFLSTR Profiler, Profiler Plus, and Identifiler kits. Novel mini-haplotype analysis that compares matches in all three-locus combinations of alleles was developed and used in the analysis of inbreeding in the group of 295 unrelated individuals. While comparing 98 skeletal remains exhumed in the process of identification of war victims in Croatia with over 3,000 genotypes of relatives of missing persons, we revealed 20 cases of 14-locus matches and 4 cases of 15-locus matches between unrelated people. We hypothesized that this unexpectedly high number of false matches might be a consequence of local inbreeding and supported this hypothesis with very low correlation between the probability of...
The purpose of this study was to determine prevalence of Chlamydia trachomatis (Ct) urogenital in... more The purpose of this study was to determine prevalence of Chlamydia trachomatis (Ct) urogenital infection and its serotype distribution from clinical samples in north-eastern Croatia. During a 3-year period, 2,379 urogenital samples were analyzed by real-time polymerase chain reaction (A group), while 4,846 genital swabs were analyzed by direct fluorescent antibody test (B group). 132 Ct positive specimens were genotyped by omp1 gene sequencing. The prevalence rate of Ct was 3.2 % in A and 1 % in B group. The most prevalent chlamydial genotype was E (44 %), followed by F (33 %), K (11.5 %), G (8 %), J/UW (5.3 %), D-IC (4.4 %), D-B120 (1.8 %), and B/IU, J/IU, Ia/IU (0.9 % each) serotypes. Single-nucleotide polymorphisms (SNPs) of omp1 gene were detected in E, K, and G serotypes. Some of these SNPs (C/T at position 272 and G/A at position 813 in E strain; C/T at position 884 in D strain) might represent novel omp1 variants.
To investigate the association of nephrolithiasis and solute carrier family 2, facilitated glucos... more To investigate the association of nephrolithiasis and solute carrier family 2, facilitated glucose transporter, member 9 (SLC2A9), also known as glucose transporter type 9, Glut9.
Aiming to evaluate the effects of population substructure on the reliability of a DNA corresponde... more Aiming to evaluate the effects of population substructure on the reliability of a DNA correspondence in the process of human identification, we used the model of ''in silico'' constructed populations with and without substructure. Effects of population substructure were evaluated at the level of locus heterozygosity, Hardy-Weinberg equilibrium and mini-haplotype distribution. Inbreeding in a subpopulation of 100 individuals through 10 generations did not significantly alter the level of heterozygosity and Hardy-Weinberg equilibrium. However, analysis of mini-haplotype distribution revealed a significant homogenization in separated subpopulations. Average observed mini-haplotype frequency (f o ) increased to threefold from expected values (f e ), and the number of mini-haplotypes with f o ⁄ f e above 10 increased over sixfold, suggesting that the effects of population substructure on calculated likelihood ratios (LR) might be larger than previously estimated. In most criminal cases, this would not represent a problem, whereas for identifications in large-scale mass fatality events, population substructure might considerably increase the risk of false identification.
International Journal of Infectious Diseases, 2008
Background: Sexually transmitted infections (STIs) constitute a significant public health concern... more Background: Sexually transmitted infections (STIs) constitute a significant public health concern, especially in resource poor settings. Notification and medical evaluation of recent sexual partners of infected patients is important for STI control. However, research on partner notification effectiveness in resource poor settings is very limited.
The result of empirical testing of forensic DNA match probabilities for Croatia is reported. It i... more The result of empirical testing of forensic DNA match probabilities for Croatia is reported. It is concluded that if consideration is given to relatedness and subpopulation effects the model of Balding and Nichols appears to give very good predictions.
The purpose of this study was to determine prevalence of Chlamydia trachomatis (Ct) urogenital in... more The purpose of this study was to determine prevalence of Chlamydia trachomatis (Ct) urogenital infection and its serotype distribution from clinical samples in north-eastern Croatia. During a 3-year period, 2,379 urogenital samples were analyzed by real-time polymerase chain reaction (A group), while 4,846 genital swabs were analyzed by direct fluorescent antibody test (B group). 132 Ct positive specimens were genotyped by omp1 gene sequencing. The prevalence rate of Ct was 3.2 % in A and 1 % in B group. The most prevalent chlamydial genotype was E (44 %), followed by F (33 %), K (11.5 %), G (8 %), J/UW (5.3 %), D-IC (4.4 %), D-B120 (1.8 %), and B/IU, J/IU, Ia/IU (0.9 % each) serotypes. Single-nucleotide polymorphisms (SNPs) of omp1 gene were detected in E, K, and G serotypes. Some of these SNPs (C/T at position 272 and G/A at position 813 in E strain; C/T at position 884 in D strain) might represent novel omp1 variants.
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