Ron Oost

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Wageningen University and Research Centre

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Papers by Ron Oost

Bioassays voor neuroactieve stoffen in water

Met bijdragen van Jessica Legradi (VU), Harm Heusinkveld (RIVM), Nynke Kramer (UU), Remco Westeri... more

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Fish biomarkers and environmental risk assessment: a review

Toxicological profiling of water samples with in vitro bioassays and assessment using effect-based trigger values

In vitro bioassays including cell-based bioassays and low-complexity whole-organism assays have b... more In vitro bioassays including cell-based bioassays and low-complexity whole-organism assays have been applied for decades in water quality monitoring. However, there is no common understanding what level or response is acceptable. As of now, bioassay results were only benchmarked against each other but not against an absolute measure of chemical water quality. The EU environmental quality standards (EQS) differentiate between poor and acceptable surface water concentrations for individual chemicals of concern but cannot capture the thousands of chemicals that are in water and their biological action as mixtures. We developed a method that reads across from existing EQS and makes additional mixture considerations to assure that the derived EBT are protective for complex mixtures as they occur in surface water. The EBT derivation method was applied to 48 in vitro bioassays with 37 of them having sufficient information to yield preliminary EBTs. 30 of those were considered robust enough...

The NORMAN interlaboratory study on biotesting of spiked water extracts

25. SETAC Europe annual meeting, May 3, 2015

The NORMAN network is a permanent self-sustaining network for the monitoring and biomonitoring of... more The NORMAN network is a permanent self-sustaining network for the monitoring and biomonitoring of emerging environmental contaminants. The NORMAN working group on Bioassays (Bio WG) focuses on the application of bioanalytical tools for environmental quality monitoring. A main objective is to provide recommendations for the implementation of effect-based tools into regulatory frameworks. In this context, a blind interlaboratory study (ILS) was performed. The aim was to verify if a bioassay battery conducted in different laboratories following own protocols would produce comparable results when testing spiked surface water extracts. The lead in planning and organizing was taken by the Department of Ecosystem Analysis (ESA), RWTH Aachen University (DE). The ILS bioassay battery included acute-toxicity assays with organisms from different trophic levels (Algae, Daphnia, Zebrafish embryos); and mechanism-specific bioassays for estrogenicity (YES, ER-Luc cell lines) and mutagenicity (Ames fluctuation) assessment. Three to four participants performed each bioassay, including: BfG (DE), Waternet (NL), Waterproef (NL), INERIS (FR), IFREMER (FR), RECETOX (CZ), ISSeP (BE), ITM (SE), IVM-VU (NL), Entox/University of Queensland (AU), Ecotox Centre (CH), ESA-RWTH (DE). Clean water from a reference site was concentrated 10.000 times with large volume solid-phase extraction. Four emerging pollutants were used for spiking: triclosan, acridine, 3-nitrobezanthrone and 17-alpha-ethinylestradiol. Extracts were spiked with either single chemicals or a mixture, in concentrations aimed to produce full dose-response curves in bioassays. The spiked extracts were prepared, separated in aliquots, identified with codes, and sent to the participating laboratories. Standardized bioassay methods (OECD, ISO) were recommended but not mandatory, and biotesters could use their own protocols. Results were sent to RWTH, and a summary of the full ILS was provided to the ILS participants. In October 2014, a workshop was held at RWTH Aachen to present and discuss the ILS results. Bioassays produced mostly highly comparable results, even when protocols differed significantly. Suggestions for future improvements include harmonization of methods for data analysis and results evaluation. An important expected outcome of the ILS is the promotion of biotesting for water quality monitoring at the level of European policy-makers

Hormoonverstoring bij vis, punt van aandacht in het zuiveringsmoeras

Challenges and technological approaches for tackling emerging contaminants in drinking and wastewater

Ulf Stein, Evelyn Lukat, Theo van den Hoven, Gerard van den Berg3, Anna Szendrenyi, Erwin Beerend... more Ulf Stein, Evelyn Lukat, Theo van den Hoven, Gerard van den Berg3, Anna Szendrenyi, Erwin Beerendonk3, Bart van der Burg, Marc Bourgin, Roberta Caris-Hofman3, Thomas Gross, Stephan Hannappel, Armelle Hebert, Marta Hernández, Hein de Jonge, Cornelia Kienle, Jörg Gebhardt, Anna Kounina, Beatriz de la Loma Gonzalez, Christa S. McArdell, Daniel Mutz, Ron van der Oost, Miranda Pieron, Christian Remy, Eszter Simon, Christoph Sprenger, Ester Vilanova, Kristina Wencki, Mariëlle van der Zouwen, Christoph Hugi, Christopher Oberschelp, Merijn Schriks, Kirsten Baken, Hannes Schritt

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Review article Fish bioaccumulation and biomarkers in environmental risk assessment: a review

Models of stickleback transcriptomics as tools to assess waste water purification

The Impact of Time-Integrating Active or Passive Sampling Methods on Bioanalytical Water Quality Risk Assessment

SSRN Electronic Journal, 2021

Paper: FISH BIOACCUMULATION AND BIOMARKERS IN ENVIRONMENTAL RISK ASSESSMENT: A REVIEW

Identifying adverse outcome pathways (AOP) for Amsterdam city fish by integrated field monitoring

Environmental Toxicology and Pharmacology

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Bioaccumulation of organic micropollutants in different aquatic organisms: sublethal toxic effects on fish

… toxicology and risk …, 1991

Ron van der Oost, 1* Henk Heida, 1 Antoon Opperhuizen, 2 and Nico PE Vermeulen3 Bioaccumulation o... more

Bioanalytical Techniques Used in Environmental Risk Assessment

Environmental Toxicology and Pharmacology

Development of a framework to derive effect-based trigger values to interpret CALUX data for drinking water quality

Water Research

Comparative field study on bioassay responses and micropollutant uptake of POCIS, Speedisk and SorbiCell polar passive samplers

Environmental Toxicology and Pharmacology

Effect-based trigger values for in vitro and in vivo bioassays performed on surface water extracts supporting the environmental quality standards (EQS) of the European Water Framework Directive

The Science of the total environment, Jan 14, 2018

Effect-based methods including cell-based bioassays, reporter gene assays and whole-organism assa... more Effect-based methods including cell-based bioassays, reporter gene assays and whole-organism assays have been applied for decades in water quality monitoring and testing of enriched solid-phase extracts. There is no common EU-wide agreement on what level of bioassay response in water extracts is acceptable. At present, bioassay results are only benchmarked against each other but not against a consented measure of chemical water quality. The EU environmental quality standards (EQS) differentiate between acceptable and unacceptable surface water concentrations for individual chemicals but cannot capture the thousands of chemicals in water and their biological action as mixtures. We developed a method that reads across from existing EQS and includes additional mixture considerations with the goal that the derived effect-based trigger values (EBT) indicate acceptable risk for complex mixtures as they occur in surface water. Advantages and limitations of various approaches to read across...

Simoni (Smart Integrated Monitoring) as a Novel Bioanalytical Strategy for Water Quality Assessment: Part II. Field Feasibility Survey

Environmental toxicology and chemistry, Sep 4, 2017

Since it is impossible to chemically analyze all relevant micropollutants, the implementation of ... more Since it is impossible to chemically analyze all relevant micropollutants, the implementation of bioanalytical tools is essential to estimate ecological risks of chemical mixtures in regular water monitoring programs. The first tier of the Smart Integrated Monitoring (SIMONI) strategy, which was described in part I, is based on the combination of passive sampling and bioanalytical measurements. Bioassay responses are compared to effect-based trigger values (EBT) and an overall SIMONI score on all bioassay data was designed to indicate environmental risks. The present paper is focused on analyzing the feasibility of the hazard identification tier by evaluating results of 45 field campaigns at sites with different pollution profiles near the city of Amsterdam. A Daphnia assay was performed in situ, while silicon rubber or POCIS passive sampler extracts were tested with four non-specific (daphnids, algae, bacteria and cell culture) and ten specific bioassays (nine CALUX assays and anti...

Bioassay battery interlaboratory investigation of emerging contaminants in spiked water extracts - Towards the implementation of bioanalytical monitoring tools in water quality assessment and monitoring

Water research, Jan 12, 2016

Bioassays are particularly useful tools to link the chemical and ecological assessments in water ... more Bioassays are particularly useful tools to link the chemical and ecological assessments in water quality monitoring. Different methods cover a broad range of toxicity mechanisms in diverse organisms, and account for risks posed by non-target compounds and mixtures. Many tests are already applied in chemical and waste assessments, and stakeholders from the science-police interface have recommended their integration in regulatory water quality monitoring. Still, there is a need to address bioassay suitability to evaluate water samples containing emerging pollutants, which are a current priority in water quality monitoring. The presented interlaboratory study (ILS) verified whether a battery of miniaturized bioassays, conducted in 11 different laboratories following their own protocols, would produce comparable results when applied to evaluate blinded samples consisting of a pristine water extract spiked with four emerging pollutants as single chemicals or mixtures, i.e. triclosan, acr...

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Relationships between bioaccumulation of organic trace pollutants (PCBS, OCPS and PAHS) and biochemical markers in feral eel (Anguilla anguilla); a multivariant analysis

Marine Environmental Research, 1996

Samples of sediments and eel were collected from six Amsterdam freshwater sites with different le... more Samples of sediments and eel were collected from six Amsterdam freshwater sites with different levels of pollution. All specimens of sediments and eel muscle tissue were analyzed for polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs) and polycyclic aromatic hydrocarbons (PAHs). Eel livers were isolated in order to determine levels and activities of phase I-enzymes, phase II-enzymes and anti-oxidant enzymes. The correlations between the accumulation of organic trace pollutants in eel muscle tissue and biochemical changes in the eel liver were studied with appropriate statistical methods. Levels of PCBs and OCPs in eel tissues appeared to be exposure related while levels of PAHs in muscle tissues did not correlate with those in sediments, due to fast metabolism in fish. Biota-sediment accumulation factors (BSAFs) could be determined from levels in sediments [rig/g organic matter (OM)] and eel muscle tissue [rig/g lipid weight (LW)]. Bioaccumulation patterns were both site and congener specific. Levels and activities of phase-I-enzymes (cyt P450 and bg, EROD, PROD, CYPlA, cyt c RED) were significantly induced in eel from the most polluted sites. Levels and activities of some phase II-enzymes and co-factors (GSSG, GSH/GSSG, GST, UDPGT) were altered significantly in specimens from the most polluted sites while others (GSH and GSH + GSSG) showed no effects. No significant pollution-related effects were demonstrated in the activities of the anti-oxidant enzymes. It appeared that most phase I-parameters (CYPlA, cyt bS, EROD and EROD/ cyt P450) correlated well with PCB and OCP tissue levels. A good correlation could also be demonstrated between OCP tissue levels and some phase II-parameters (GSSG, GSH/GSSG and UDPGT), while PCB levels correlated with UDPGT activity only. No correlations were found for PAH levels and anti-oxidant parameters. A correlation-significance index (CSI) was postulated in order to visualize the individual relationships between tissue levels of all congeners and the hepatic biochemical markers. With principal component analysis (PCA) and discriminant analysis a clear distinction of sites (two reference sites, three moderately polluted sites, and one heavily polluted site) was demonstrated on the first factorial plane when the sites were classified on the basis of biomarkers. These differences were less pronounced when sites were classified on the basis of accumulated organic trace pollutants in eel muscle. Residual maximum likelihood (REML) analysis revealed that hepatic phase I-enzymes not only correlated with PCB and OCP levels but that a significant correlation could also be demonstrated with fish size (length and weight). Better correlations between bioaccumulation and biomarkers were observed when fish tissue pollutant levels were expressed on a lipid weight instead of a fresh weight basis.

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Biomonitoring of aquatic pollution with feral eel (Anguilla anguilla) II. Biomarkers: pollution-induced biochemical responses

Aquatic Toxicology, 1996

... a recycling reaction followed by spectrophotometric detection in a flowinjec tion analysis sy... more

Bioassays voor neuroactieve stoffen in water

Met bijdragen van Jessica Legradi (VU), Harm Heusinkveld (RIVM), Nynke Kramer (UU), Remco Westeri... more

Download

Fish biomarkers and environmental risk assessment: a review

Toxicological profiling of water samples with in vitro bioassays and assessment using effect-based trigger values

The NORMAN interlaboratory study on biotesting of spiked water extracts

25. SETAC Europe annual meeting, May 3, 2015

Hormoonverstoring bij vis, punt van aandacht in het zuiveringsmoeras

Challenges and technological approaches for tackling emerging contaminants in drinking and wastewater

Download

Review article Fish bioaccumulation and biomarkers in environmental risk assessment: a review

Models of stickleback transcriptomics as tools to assess waste water purification

The Impact of Time-Integrating Active or Passive Sampling Methods on Bioanalytical Water Quality Risk Assessment

SSRN Electronic Journal, 2021

Paper: FISH BIOACCUMULATION AND BIOMARKERS IN ENVIRONMENTAL RISK ASSESSMENT: A REVIEW

Identifying adverse outcome pathways (AOP) for Amsterdam city fish by integrated field monitoring

Environmental Toxicology and Pharmacology

Download

Bioaccumulation of organic micropollutants in different aquatic organisms: sublethal toxic effects on fish

… toxicology and risk …, 1991

Ron van der Oost, 1* Henk Heida, 1 Antoon Opperhuizen, 2 and Nico PE Vermeulen3 Bioaccumulation o... more

Bioanalytical Techniques Used in Environmental Risk Assessment

Environmental Toxicology and Pharmacology

Development of a framework to derive effect-based trigger values to interpret CALUX data for drinking water quality

Water Research

Comparative field study on bioassay responses and micropollutant uptake of POCIS, Speedisk and SorbiCell polar passive samplers

Environmental Toxicology and Pharmacology

Effect-based trigger values for in vitro and in vivo bioassays performed on surface water extracts supporting the environmental quality standards (EQS) of the European Water Framework Directive

The Science of the total environment, Jan 14, 2018

Simoni (Smart Integrated Monitoring) as a Novel Bioanalytical Strategy for Water Quality Assessment: Part II. Field Feasibility Survey

Environmental toxicology and chemistry, Sep 4, 2017

Water research, Jan 12, 2016

Download

Relationships between bioaccumulation of organic trace pollutants (PCBS, OCPS and PAHS) and biochemical markers in feral eel (Anguilla anguilla); a multivariant analysis

Marine Environmental Research, 1996

Download

Biomonitoring of aquatic pollution with feral eel (Anguilla anguilla) II. Biomarkers: pollution-induced biochemical responses

Aquatic Toxicology, 1996

... a recycling reaction followed by spectrophotometric detection in a flowinjec tion analysis sy... more

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