Papers by Robert Gore-Langton
Acta obstetricia et gynecologica Scandinavica, 2014
Women who develop gestational diabetes mellitus or impaired glucose tolerance during pregnancy ar... more Women who develop gestational diabetes mellitus or impaired glucose tolerance during pregnancy are at substantially increased risk for type 2 diabetes and comorbidities after pregnancy. Little is known about the role of genetic factors and their interactions with environmental factors in determining the transition from gestational diabetes mellitus to overt type 2 diabetes mellitus. These critical data gaps served as the impetus for this Diabetes & Women's Health study with the overall goal of investigating genetic factors and their interactions with risk factors amenable to clinical or public health interventions in relation to the transition of gestational diabetes mellitus to type 2 diabetes mellitus. To achieve the goal efficiently, we are applying a hybrid design enrolling and collecting data longitudinally from approximately 4000 women with a medical history of gestational diabetes mellitus in two existing prospective cohorts, the Nurses' Health Study II and the Danish...
Oncology (Williston Park, N.Y.)
1. Oncology (Williston Park). 2001 Apr;15(4):456-8, 461-6. Clinical trials referral resource. Hea... more 1. Oncology (Williston Park). 2001 Apr;15(4):456-8, 461-6. Clinical trials referral resource. Health related quality of life in cancer clinical trials. Trimble EL, Rowland J, Varricchio C, Gore-Langton RE. PMID: 11346933 [PubMed - indexed for MEDLINE]. Publication Types: Directory ...
Oncology (Williston Park, N.Y.), 2001
1. Oncology (Williston Park). 2001 Oct;15(10):1284, 1286-7, 1291-2, 1294. Clinical trials referra... more 1. Oncology (Williston Park). 2001 Oct;15(10):1284, 1286-7, 1291-2, 1294. Clinical trials referral resource. Investigator-initiated health-related quality-of-life research. Rowland JH, Varricchio CG, Trimble EL, Gore-Langton RE. National Cancer Institute, USA. ...
Oncology (Williston Park, N.Y.), 2001
1. Oncology (Williston Park). 2001 Apr;15(4):456-8, 461-6. Clinical trials referral resource. Hea... more 1. Oncology (Williston Park). 2001 Apr;15(4):456-8, 461-6. Clinical trials referral resource. Health related quality of life in cancer clinical trials. Trimble EL, Rowland J, Varricchio C, Gore-Langton RE. PMID: 11346933 [PubMed - indexed for MEDLINE]. Publication Types: Directory ...
Oncology (Williston Park, N.Y.), 1995
British journal of cancer, 1973
A thin-layer gel filtration system for the assay of oestrogen receptors in target organ tissue sa... more A thin-layer gel filtration system for the assay of oestrogen receptors in target organ tissue samples as small as 50 mg is presented and compared with the sucrose gradient centrifugation method. Only specific high-affinity binding of [(3)H]17β-oestradiol to endometrial cytosol has been observed with the thin-layer gel filtration assay, despite the presence of relatively high levels of nonspecific binding components. The system could be adapted to the clinical determination of oestrogen receptor content in biopsy material from mammary and endometrial tumours and would be of value in predicting hormone dependency.
Oncology (Williston Park, N.Y.), 1991
... 2000 Apr;14(4):553-4, 559, 561. Clinical trials, referral resource. Treatment of advanced or ... more ... 2000 Apr;14(4):553-4, 559, 561. Clinical trials, referral resource. Treatment of advanced or recurrent squamous cell carcinoma of the head and neck. Conley BA, Gore-Langton RE, Cheson BD. PMID: 10826315 [PubMed - indexed for MEDLINE]. Publication Types: Directory. ...
Oncology (Williston Park, N.Y.), 2002
Reproductive BioMedicine Online, 2011
Oncology (Williston Park, N.Y.), 2005
Current Protocols in Human Genetics, 2001
Preimplantation genetic testing, which includes preimplantation genetic diagnosis (PGD) and preim... more Preimplantation genetic testing, which includes preimplantation genetic diagnosis (PGD) and preimplantation genetic screening (PGS), is a form of a very early prenatal testing. The goal of this method is to avoid transfer of embryos affected with a specific genetic disease or condition. This unit describes the steps involved in amplifying DNA from a single blastomere and specific assays for detecting a variety of DNA mutations. For some assays, whole-genome amplification by primer-extension preamplification (PEP) is performed prior to analysis. Support protocols describe the biopsy of one or two blastomeres from the developing preimplantation embryo, isolation for further investigation of all blastomeres from embryos shown to have the mutant allele, and isolation of single lymphocytes or lymphoblastoid cells as models for single-cell DNA analysis. A procedure for FISH analysis on single interphase blastomeres is provided along with support protocols for probe preparation and probe validation, which is recommended as a preliminary step before performing any PGD or PGS FISH analysis.
Molecular and Cellular Endocrinology, 1988
Potential side-effects of the immunosuppressive drug cyclosporine (also cyclosporin A, CsA) on ov... more Potential side-effects of the immunosuppressive drug cyclosporine (also cyclosporin A, CsA) on ovarian endocrine function have been investigated using granulosa cells isolated from immature estrogen-porno rats and cultured in a chemically defined medium. The FSH-dependent differentiation of steroidogenic pathways for estrogen and progestin secretion was shown to be differentially affected by CsA in vitro, at drug concentrations that approximate immunosuppressive concentrations in blood of humans or animals. CsA at 0.1-l pg/ml synergistically enhanced FSH-stimulated aromatase activity as measured by the conversion of exogenous testosterone to 17&estradiol, while production of the progestins (progesterone + 20ar-hydroxypregn-4-en-3-one + pregnenolone) was little affected at up to 0.1 rg/ml CsA and reduced at higher concentrations. CsA alone did not stimulate basal steroid secretion. The action of CsA to augment FSH-stimulated induction of aromatase activity was seen both in the presence or absence of testosterone. The effects of CsA (1 ~g/ml), either senator on aroma&se activity or i~bito~ on progestin secretion, were in general increased with greater times of cell exposure throughout the culture period, although the temporal effects on 178-estradiol and the progestins were not identical following delayed addition or removal of CsA from the culture medium. Higher concentrations of CsA (3-10 pg/ml) were generally toxic to granulosa cells as indicated by marked decreases in 17p-estradiol and progestin secretion and in inco~oration of [3H]leucine. These results suggest that therapeutic ~ncentrations of CsA might directly influence ovarian function by differentially modulating the FSH-dependent steroidogenic pathways of granulosa cells.
International Journal of Gynecology & Obstetrics, 1996
To compare IVF rates using partial zona dissection versus zona intact insemination in couples wit... more To compare IVF rates using partial zona dissection versus zona intact insemination in couples with male infertility. To analyze pregnancy rates relative to sperm characteristics, fertilization rates, and treatment. Randomized prospective comparison of fertilization in sibling oocytes. Transfer of the three best quality embryos from one or both treatments. Department of Gynaecology and Reproductive Medicine, University Hospital, London, Ontario, Canada. Thirty-two couples undergoing IVF with a principal diagnosis of male infertility. Treatment with partial zona dissection. Fertilization and pregnancy. Fertilization rates were 26% and 9% after partial zona dissection and IVF, respectively. Polyspermy was < 1% in each treatment. There were five singleton pregnancies in 29 completed cycles, three in cycles with fertilization only by partial zona dissection and two in cycles with both partial zona dissection and IVF fertilization. There were no pregnancies after fertilization by IVF only. Stepwise logistic regression analysis indicated that pregnancy was related to partial zona dissection, initial sperm concentration, and total acrosin activity. Partial zona dissection was associated with minimal polyspermic fertilization and higher normal fertilization rates than sibling oocytes treated by modified IVF. Pregnancy occurred only after transfer of embryos from partial zona dissection or combined partial zona dissection and IVF.
Genetic Testing, 1997
Mutations in the genes BRCA1 and BRCA2 account for 5%-10% of familial early onset breast cancer. ... more Mutations in the genes BRCA1 and BRCA2 account for 5%-10% of familial early onset breast cancer. Identification of these mutations allows molecular diagnosis for breast cancer susceptibility. A high through-put automated PCR allelic discrimination assay (ADA) was developed to detect the prevalent mutations in these genes. Two allele specific oligonucleotides (ASO) are directly used in the PCR reaction, in both of which the fluorescent reporter and quencher dyes are attached to the 5' and 3' ends, respectively. During PCR, fluorescence is generated after cleavage of the annealed ASO by the 5' nuclease activity of Taq polymerase. The wild-type BRCA sequence is distinguished from the mutant sequence by the differential fluorescence emission of two different reporter dyes. The sensitivity of ADA is at the level of a single cell following a nested PCR. Eighty-six patient samples can be analyzed for each mutation in 15-min post-PCR without the need for radioactivity, gel electrophoresis, or membrane blotting/hybridization.
Biology of Reproduction, 1990
Formation of a fluid-tilled antrum results from the actions of FSH and estrogen on preantral ovar... more Formation of a fluid-tilled antrum results from the actions of FSH and estrogen on preantral ovarian fofficles in most mammalian species.
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Papers by Robert Gore-Langton