This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Background: In this study, column chromatography was applied to separate active fractions from th... more Background: In this study, column chromatography was applied to separate active fractions from the ethyl acetate extract of Celastrus hindsii, a medicinal plant widely used in Southern China, Northern Vietnam, Myanmar, and Malaysia. Methods: Fourteen fractions from different dilutions of chloroform and methanol were separated by column chromatography and examined for biological activities. Results: It was found that a dilution of 50-70% methanol in chloroform yielded the highest total phenolics, flavonoids, and antioxidant activities (1,1-dipheny1-2-picrylhydrazyl (DPPH), 2,2-azinobis (3-ehtylbenzothiazoline-6-sulfonic acid), diammonium salt (ABTS) radical scavenging activity, and β-carotene bleaching method measured by lipid peroxidation inhibition). In addition, by gas chromatography-mass spectrometry (GC-MS) and electrospray ionization-mass spectrometry (ESI-MS) analyses, fifteen principal compounds from bioactive fractions belonging to fatty acids, amides, flavonoids, sterols, terpenes, and phenols were identified. Of these compounds, α-amyrin, β-amyrin, hydrazine carboxamide, hexadecanoic acid, fucosterol, (3β)-D:C-friedours-7-en-3-ol, rutin, and 2-hydroxy-1-ethyl ester accounted for maximal quantities, whilst concentrations of other constituents were <5%. Conclusions: It is suggested that these identified compounds may greatly contribute to the antioxidant capacity of C. hindsii as well as its potential pharmaceutical properties.
International Letters of Natural Sciences, Jan 10, 2020
Chilling is one of the major abiotic stress which limites yield and quality of many crops. The se... more Chilling is one of the major abiotic stress which limites yield and quality of many crops. The seedlings of rice varieties namely Koshihikari (Japonica subtype) chilling tolerant, and the susceptible NMR2 (Indica subtype) were treated at 25/15 °C and 5/4 °C day/night to determine the growth parameters, phenolic contents, and antioxidant activity. It was found that in all treatments, the growth of MNR2 including root and shoot lengths, and leaf and root weights were inhibited at greater levels than Koshihikari. There were seven phenolic acids identified in leaves of Koshihikari including caffeic acid, vanillin, ferulic acid, sinapic acid, benzoic acid, ellagic acid, and cinamic acid, but only benzoic acid and ellagic acid were found in leaves of MNR2. In contrast, only vanillic acid and ellagic acid were observed in roots of Koshihikari, whilst ellagic acid and cinnamic acid were found in roots of MNR2. It was found that rice reduced amount of phenolic acids but promoted quantity of total phenolic content (TPC) and total flavonoid content (TFC) and level of antioxidant activity in chilling stress, although the level of responses varied between Japonica and Indica subtypes. Tolerant rice possessed greater bound flavonoids, phenolics and phenolic acids, but susceptible rice accumulated greater free TPC and TFC in reduced temperature. Findings of this study highlighted that phenolic constituents in bound forms of phenols, polyphenols, and flavonoids may play an active role in rice plants than phenolic acids under chilling stress but need further elaboration.
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
The management of amylose and protein contents and cooking quality are the main challenges in ric... more The management of amylose and protein contents and cooking quality are the main challenges in rice macronutrients and quality improvement. This experiment was conducted to examine the rice grain quality, alkali digestion, and gel consistency responses to irrigation interval after anthesis. Three rice varieties (K1, K3, and K4) were subjected to different irrigation intervals (1, 2, and 3 d) after anthesis. The findings of this study showed that the protein content was markedly increased from 6.53–6.63% to 9.93–10.16%, whilst the amylose content was decreased significantly from 22.00–22.43% to 16.33–17.56% under stressed treatments at irrigation intervals, whilst the quantity of fatty acids was not affected. The 3-d irrigation interval recorded the highest protein content but the lowest amylose value. In addition, this treatment shows lower gelatinization temperature, but it is negatively associated with hard gel consistency under irrigation interval. This study highlights that the w...
Chilling is one of the major abiotic stress which limites yield and quality of many crops. The se... more Chilling is one of the major abiotic stress which limites yield and quality of many crops. The seedlings of rice varieties namely Koshihikari (Japonica subtype) chilling tolerant, and the susceptible NMR2 (Indica subtype) were treated at 25/15 °C and 5/4 °C day/night to determine the growth parameters, phenolic contents, and antioxidant activity. It was found that in all treatments, the growth of MNR2 including root and shoot lengths, and leaf and root weights were inhibited at greater levels than Koshihikari. There were seven phenolic acids identified in leaves of Koshihikari including caffeic acid, vanillin, ferulic acid, sinapic acid, benzoic acid, ellagic acid, and cinamic acid, but only benzoic acid and ellagic acid were found in leaves of MNR2. In contrast, only vanillic acid and ellagic acid were observed in roots of Koshihikari, whilst ellagic acid and cinnamic acid were found in roots of MNR2. It was found that rice reduced amount of phenolic acids but promoted quantity of ...
Background: In this study, column chromatography was applied to separate active fractions from th... more Background: In this study, column chromatography was applied to separate active fractions from the ethyl acetate extract of Celastrus hindsii, a medicinal plant widely used in Southern China, Northern Vietnam, Myanmar, and Malaysia. Methods: Fourteen fractions from different dilutions of chloroform and methanol were separated by column chromatography and examined for biological activities. Results: It was found that a dilution of 50–70% methanol in chloroform yielded the highest total phenolics, flavonoids, and antioxidant activities (1,1-dipheny1-2-picrylhydrazyl (DPPH), 2,2-azinobis (3-ehtylbenzothiazoline-6-sulfonic acid), diammonium salt (ABTS) radical scavenging activity, and β-carotene bleaching method measured by lipid peroxidation inhibition). In addition, by gas chromatography-mass spectrometry (GC-MS) and electrospray ionization-mass spectrometry (ESI-MS) analyses, fifteen principal compounds from bioactive fractions belonging to fatty acids, amides, flavonoids, sterols, t...
The present study was conducted to evaluate the antioxidant activity, grain traits and quality co... more The present study was conducted to evaluate the antioxidant activity, grain traits and quality contents in Afghanistan’s selected rice varieties. The antioxidant capacity was measured using DPPH (1,1-diphenyl-2-picrylhydrazyl) and Reducing Power methods. Grain length, grain width and grain thickness were in the range of 5.4-7.6, 1.7-3.2, and 1.5-2.2 mm respectively. Selah Zoodras, Taram Irani and Attai-1 had the longest grain length whereas Luke was associated with high width and thickness among the varieties. Protein, amylose and lipid content varied from 6.5-9.9, 20.3-23.5, and 10-18% respectively. Barah Kunduz showed the highest protein and lipid content while Selah zoodras was associated highest amylose content. The selected rice varieties possessed moderate antioxidant activity and their physiochemical properties were not correlated with antioxidant capacity. Results of this study provided useful information on the selection and production of rice varieties with high nutritiona...
The control of protein and amylose content is the principal challenge in rice nutrient and qualit... more The control of protein and amylose content is the principal challenge in rice nutrient and quality improvement. In this study, water deficits in 2- and 3-day intervals were imposed on two Japonica cultivars K1 and K3, and an Indica K4 subtype after anthesis to harvest. It was observed that although rice yield was affected, the protein content was increased 6.53–6.63% to 9.93–10.16%. The amylose quantity was reduced significantly from 22.00–22.43% to 16.33–17.56%, while fatty acids in rice grain were not influenced. Total anthocyanins were greatly promoted by 53.1% as compared to the non-treated trials. The antioxidant capacity in rice grain increased up to 59.1% in 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and 41.6% in reducing power assays. Findings of this research revealed that the 3-day interval of water deficit imposition was the most effective to improve rice macronutrients and quality, as well as beneficial phytochemicals and antioxidants in rice grain. The wate...
This study was conducted to develop the salt tolerance of rice by exogenous application of magnes... more This study was conducted to develop the salt tolerance of rice by exogenous application of magnesium sulfate supplement (MgSO4). The salinization was carried out on 7-day-old rice seedlings including BC15 (salinity tolerant) and DT84DB (salinity susceptible) varieties with 0.5 mM MgSO4. The exogenous application of MgSO4 significantly improves the growth of seedlings of both varieties. In addition, antioxidant activities increase in line with the raise of total phenolic and total flavonoid contents. Remarkably, the contents of momilactone B (MB) and phenolic compounds including tricin, ρ-coumaric, salicylic, cinnamic, benzoic, and ferulic acids simultaneously rise in both varieties treated by salinity and 0.5 mM MgSO4. Interestingly, MB was not found in the salt-treated samples but presents with considerable contents in the salt and MgSO4-treated cultivars. The findings imply that MgSO4 may significantly improve the salt tolerance of rice seedlings through the enhancement of seconda...
International Journal of Environment, Agriculture and Biotechnology, 2017
Advanced drought tolerant lines were analysed for blast disease, brown planthopper (BPH), and phy... more Advanced drought tolerant lines were analysed for blast disease, brown planthopper (BPH), and phytic acid content. Thirsty lines of BC2F4 derived from OMCS2000/ IR75499-73-1 were used to screen for BPH and blast resistance. Three good resistant lines were screened against blast (45, 54, and 310) under greenhouse condition. As eight lines were identified to be resistant to BPH. The results further reveal that BC2F4-45 was the best line resistant to both BPH and blast disease. These lines will be useful in reducing grain phytic acid and improving the nutritional value of rice grain. Based on an assay for high phosphate germination stage of rice, the lowest content was found in the I5 variety (line 45). Hence, this line provides the urgent objective for breeders in cultivars of these crops to genetically enhance a healthy and functional diet. These characters will then need to be incorporated into high yield under drought stress with others such as disease and insect resistance.
China is a water shortage country. The paper analyzes the current situation and problems of water... more China is a water shortage country. The paper analyzes the current situation and problems of water resources in both urban and rural areas. To solve the problems, the paper puts forward five countermeasures and draws up structure chart. It shows specific contents and mutual relationships of each countermeasure clearly, and provides the basis for sustainable development of urban and rural water resources. It can be used in engineering practice effectively.
Improved food security remains a major challenge for policymakers in Afghanistan. The objective o... more Improved food security remains a major challenge for policymakers in Afghanistan. The objective of this study is to investigate the prevalence and drivers of food insecurity among farming households in the Takhar region of Afghanistan. Household questionnaire survey datasets were obtained from 262 farmers via multi-stage sampling across four districts. The Household Food Insecurity Access Scale (HFIAS) was constructed to assess the prevalence of food insecurity, and an ordered probit econometric model was used to identify the factors influencing the prevalence rates in farming families. The findings indicate that 66.79% of the farming households were food insecure, whilst 30.53% were severely food insecure. After adjustment of socio-economic and demographic characteristics of families, the ordered probit results reveals that household head education, dependency ratio, farm income, access to non-agricultural income, livestock unit, group membership, borrowing, farm diseases, flood, a...
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Background: In this study, column chromatography was applied to separate active fractions from th... more Background: In this study, column chromatography was applied to separate active fractions from the ethyl acetate extract of Celastrus hindsii, a medicinal plant widely used in Southern China, Northern Vietnam, Myanmar, and Malaysia. Methods: Fourteen fractions from different dilutions of chloroform and methanol were separated by column chromatography and examined for biological activities. Results: It was found that a dilution of 50-70% methanol in chloroform yielded the highest total phenolics, flavonoids, and antioxidant activities (1,1-dipheny1-2-picrylhydrazyl (DPPH), 2,2-azinobis (3-ehtylbenzothiazoline-6-sulfonic acid), diammonium salt (ABTS) radical scavenging activity, and β-carotene bleaching method measured by lipid peroxidation inhibition). In addition, by gas chromatography-mass spectrometry (GC-MS) and electrospray ionization-mass spectrometry (ESI-MS) analyses, fifteen principal compounds from bioactive fractions belonging to fatty acids, amides, flavonoids, sterols, terpenes, and phenols were identified. Of these compounds, α-amyrin, β-amyrin, hydrazine carboxamide, hexadecanoic acid, fucosterol, (3β)-D:C-friedours-7-en-3-ol, rutin, and 2-hydroxy-1-ethyl ester accounted for maximal quantities, whilst concentrations of other constituents were <5%. Conclusions: It is suggested that these identified compounds may greatly contribute to the antioxidant capacity of C. hindsii as well as its potential pharmaceutical properties.
International Letters of Natural Sciences, Jan 10, 2020
Chilling is one of the major abiotic stress which limites yield and quality of many crops. The se... more Chilling is one of the major abiotic stress which limites yield and quality of many crops. The seedlings of rice varieties namely Koshihikari (Japonica subtype) chilling tolerant, and the susceptible NMR2 (Indica subtype) were treated at 25/15 °C and 5/4 °C day/night to determine the growth parameters, phenolic contents, and antioxidant activity. It was found that in all treatments, the growth of MNR2 including root and shoot lengths, and leaf and root weights were inhibited at greater levels than Koshihikari. There were seven phenolic acids identified in leaves of Koshihikari including caffeic acid, vanillin, ferulic acid, sinapic acid, benzoic acid, ellagic acid, and cinamic acid, but only benzoic acid and ellagic acid were found in leaves of MNR2. In contrast, only vanillic acid and ellagic acid were observed in roots of Koshihikari, whilst ellagic acid and cinnamic acid were found in roots of MNR2. It was found that rice reduced amount of phenolic acids but promoted quantity of total phenolic content (TPC) and total flavonoid content (TFC) and level of antioxidant activity in chilling stress, although the level of responses varied between Japonica and Indica subtypes. Tolerant rice possessed greater bound flavonoids, phenolics and phenolic acids, but susceptible rice accumulated greater free TPC and TFC in reduced temperature. Findings of this study highlighted that phenolic constituents in bound forms of phenols, polyphenols, and flavonoids may play an active role in rice plants than phenolic acids under chilling stress but need further elaboration.
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
The management of amylose and protein contents and cooking quality are the main challenges in ric... more The management of amylose and protein contents and cooking quality are the main challenges in rice macronutrients and quality improvement. This experiment was conducted to examine the rice grain quality, alkali digestion, and gel consistency responses to irrigation interval after anthesis. Three rice varieties (K1, K3, and K4) were subjected to different irrigation intervals (1, 2, and 3 d) after anthesis. The findings of this study showed that the protein content was markedly increased from 6.53–6.63% to 9.93–10.16%, whilst the amylose content was decreased significantly from 22.00–22.43% to 16.33–17.56% under stressed treatments at irrigation intervals, whilst the quantity of fatty acids was not affected. The 3-d irrigation interval recorded the highest protein content but the lowest amylose value. In addition, this treatment shows lower gelatinization temperature, but it is negatively associated with hard gel consistency under irrigation interval. This study highlights that the w...
Chilling is one of the major abiotic stress which limites yield and quality of many crops. The se... more Chilling is one of the major abiotic stress which limites yield and quality of many crops. The seedlings of rice varieties namely Koshihikari (Japonica subtype) chilling tolerant, and the susceptible NMR2 (Indica subtype) were treated at 25/15 °C and 5/4 °C day/night to determine the growth parameters, phenolic contents, and antioxidant activity. It was found that in all treatments, the growth of MNR2 including root and shoot lengths, and leaf and root weights were inhibited at greater levels than Koshihikari. There were seven phenolic acids identified in leaves of Koshihikari including caffeic acid, vanillin, ferulic acid, sinapic acid, benzoic acid, ellagic acid, and cinamic acid, but only benzoic acid and ellagic acid were found in leaves of MNR2. In contrast, only vanillic acid and ellagic acid were observed in roots of Koshihikari, whilst ellagic acid and cinnamic acid were found in roots of MNR2. It was found that rice reduced amount of phenolic acids but promoted quantity of ...
Background: In this study, column chromatography was applied to separate active fractions from th... more Background: In this study, column chromatography was applied to separate active fractions from the ethyl acetate extract of Celastrus hindsii, a medicinal plant widely used in Southern China, Northern Vietnam, Myanmar, and Malaysia. Methods: Fourteen fractions from different dilutions of chloroform and methanol were separated by column chromatography and examined for biological activities. Results: It was found that a dilution of 50–70% methanol in chloroform yielded the highest total phenolics, flavonoids, and antioxidant activities (1,1-dipheny1-2-picrylhydrazyl (DPPH), 2,2-azinobis (3-ehtylbenzothiazoline-6-sulfonic acid), diammonium salt (ABTS) radical scavenging activity, and β-carotene bleaching method measured by lipid peroxidation inhibition). In addition, by gas chromatography-mass spectrometry (GC-MS) and electrospray ionization-mass spectrometry (ESI-MS) analyses, fifteen principal compounds from bioactive fractions belonging to fatty acids, amides, flavonoids, sterols, t...
The present study was conducted to evaluate the antioxidant activity, grain traits and quality co... more The present study was conducted to evaluate the antioxidant activity, grain traits and quality contents in Afghanistan’s selected rice varieties. The antioxidant capacity was measured using DPPH (1,1-diphenyl-2-picrylhydrazyl) and Reducing Power methods. Grain length, grain width and grain thickness were in the range of 5.4-7.6, 1.7-3.2, and 1.5-2.2 mm respectively. Selah Zoodras, Taram Irani and Attai-1 had the longest grain length whereas Luke was associated with high width and thickness among the varieties. Protein, amylose and lipid content varied from 6.5-9.9, 20.3-23.5, and 10-18% respectively. Barah Kunduz showed the highest protein and lipid content while Selah zoodras was associated highest amylose content. The selected rice varieties possessed moderate antioxidant activity and their physiochemical properties were not correlated with antioxidant capacity. Results of this study provided useful information on the selection and production of rice varieties with high nutritiona...
The control of protein and amylose content is the principal challenge in rice nutrient and qualit... more The control of protein and amylose content is the principal challenge in rice nutrient and quality improvement. In this study, water deficits in 2- and 3-day intervals were imposed on two Japonica cultivars K1 and K3, and an Indica K4 subtype after anthesis to harvest. It was observed that although rice yield was affected, the protein content was increased 6.53–6.63% to 9.93–10.16%. The amylose quantity was reduced significantly from 22.00–22.43% to 16.33–17.56%, while fatty acids in rice grain were not influenced. Total anthocyanins were greatly promoted by 53.1% as compared to the non-treated trials. The antioxidant capacity in rice grain increased up to 59.1% in 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and 41.6% in reducing power assays. Findings of this research revealed that the 3-day interval of water deficit imposition was the most effective to improve rice macronutrients and quality, as well as beneficial phytochemicals and antioxidants in rice grain. The wate...
This study was conducted to develop the salt tolerance of rice by exogenous application of magnes... more This study was conducted to develop the salt tolerance of rice by exogenous application of magnesium sulfate supplement (MgSO4). The salinization was carried out on 7-day-old rice seedlings including BC15 (salinity tolerant) and DT84DB (salinity susceptible) varieties with 0.5 mM MgSO4. The exogenous application of MgSO4 significantly improves the growth of seedlings of both varieties. In addition, antioxidant activities increase in line with the raise of total phenolic and total flavonoid contents. Remarkably, the contents of momilactone B (MB) and phenolic compounds including tricin, ρ-coumaric, salicylic, cinnamic, benzoic, and ferulic acids simultaneously rise in both varieties treated by salinity and 0.5 mM MgSO4. Interestingly, MB was not found in the salt-treated samples but presents with considerable contents in the salt and MgSO4-treated cultivars. The findings imply that MgSO4 may significantly improve the salt tolerance of rice seedlings through the enhancement of seconda...
International Journal of Environment, Agriculture and Biotechnology, 2017
Advanced drought tolerant lines were analysed for blast disease, brown planthopper (BPH), and phy... more Advanced drought tolerant lines were analysed for blast disease, brown planthopper (BPH), and phytic acid content. Thirsty lines of BC2F4 derived from OMCS2000/ IR75499-73-1 were used to screen for BPH and blast resistance. Three good resistant lines were screened against blast (45, 54, and 310) under greenhouse condition. As eight lines were identified to be resistant to BPH. The results further reveal that BC2F4-45 was the best line resistant to both BPH and blast disease. These lines will be useful in reducing grain phytic acid and improving the nutritional value of rice grain. Based on an assay for high phosphate germination stage of rice, the lowest content was found in the I5 variety (line 45). Hence, this line provides the urgent objective for breeders in cultivars of these crops to genetically enhance a healthy and functional diet. These characters will then need to be incorporated into high yield under drought stress with others such as disease and insect resistance.
China is a water shortage country. The paper analyzes the current situation and problems of water... more China is a water shortage country. The paper analyzes the current situation and problems of water resources in both urban and rural areas. To solve the problems, the paper puts forward five countermeasures and draws up structure chart. It shows specific contents and mutual relationships of each countermeasure clearly, and provides the basis for sustainable development of urban and rural water resources. It can be used in engineering practice effectively.
Improved food security remains a major challenge for policymakers in Afghanistan. The objective o... more Improved food security remains a major challenge for policymakers in Afghanistan. The objective of this study is to investigate the prevalence and drivers of food insecurity among farming households in the Takhar region of Afghanistan. Household questionnaire survey datasets were obtained from 262 farmers via multi-stage sampling across four districts. The Household Food Insecurity Access Scale (HFIAS) was constructed to assess the prevalence of food insecurity, and an ordered probit econometric model was used to identify the factors influencing the prevalence rates in farming families. The findings indicate that 66.79% of the farming households were food insecure, whilst 30.53% were severely food insecure. After adjustment of socio-economic and demographic characteristics of families, the ordered probit results reveals that household head education, dependency ratio, farm income, access to non-agricultural income, livestock unit, group membership, borrowing, farm diseases, flood, a...
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