Variability among 44 isolates of Fusarium spp. the Chilli wilt pathogen, collected from different... more Variability among 44 isolates of Fusarium spp. the Chilli wilt pathogen, collected from different locations of south India was studied in respect of Cultural and morphological and pathogenic variability. The colony diameter ranged from 60 mm to 90 mm after 8 days of inoculation incubated at 27±1°C. The colony colour varied from white, cream and violet with yellow coloured pigmentation on Potato dextrose agar. The growth on different solid media revealed that the best media was Potato dextrose agar (90 mm) with abundant sporulation followed by oat meal agar and V-8 juice agar. The colour of the colony also varied from white, cottony, cream and greyish in different media. As for the pathogenic variability is concerned the isolates showed variations in causing disease in different varieties of chilli and these isolates were clustered into three groups based the disease reaction.
The wilt caused by bacteria (Ralstonia solanacearum) is the most devastating pathogen among all t... more The wilt caused by bacteria (Ralstonia solanacearum) is the most devastating pathogen among all the rhizome rot pathogens. An effort was made for quick detection of bacterial wilt pathogen using PCR based molecular techniques. Phylogram results of four RS isolates Revealed that there are two major clusters, cluster A comprising all Ralstonia solanacearum species, A glance towards dendrogram reveals that there was not much diversity among the four isolates of RS. The fliC gene was amplified and an expected size of 400bp it was confirmed the Ralstonia solanacearum flagellin protein (fliC) gene. Fragment of fliC gene sequences of RS-1 (Haveri) and RS-8 (Shimoga) isolates showed 99 per cent similarity with NCBI published sequence of RS Strain MR11 plasmid mega plasmid flagellin protein (fliC) gene, partial cds (KF031064.1) and DSM9544 flagellin (fliC) gene, partial cds (AY192724.1) respectively. Dharwad isolate showed 98 per cent similarity with Ralstonia solanacearum strain RCR-226 flagellin protein (fliC) gene, partial cds (KC834785.1). While Uttara Kannada isolate showe 97 per cent similarity with RS flagellin (fliC) gene, complete cds (AF283285.1).
Variability among 44 isolates of Fusarium spp. the Chilli wilt pathogen, collected from different... more Variability among 44 isolates of Fusarium spp. the Chilli wilt pathogen, collected from different locations of south India was studied in respect of Cultural and morphological and pathogenic variability. The colony diameter ranged from 60 mm to 90 mm after 8 days of inoculation incubated at 27±1°C. The colony colour varied from white, cream and violet with yellow coloured pigmentation on Potato dextrose agar. The growth on different solid media revealed that the best media was Potato dextrose agar (90 mm) with abundant sporulation followed by oat meal agar and V-8 juice agar. The colour of the colony also varied from white, cottony, cream and greyish in different media. As for the pathogenic variability is concerned the isolates showed variations in causing disease in different varieties of chilli and these isolates were clustered into three groups based the disease reaction.
The wilt caused by bacteria (Ralstonia solanacearum) is the most devastating pathogen among all t... more The wilt caused by bacteria (Ralstonia solanacearum) is the most devastating pathogen among all the rhizome rot pathogens. An effort was made for quick detection of bacterial wilt pathogen using PCR based molecular techniques. Phylogram results of four RS isolates Revealed that there are two major clusters, cluster A comprising all Ralstonia solanacearum species, A glance towards dendrogram reveals that there was not much diversity among the four isolates of RS. The fliC gene was amplified and an expected size of 400bp it was confirmed the Ralstonia solanacearum flagellin protein (fliC) gene. Fragment of fliC gene sequences of RS-1 (Haveri) and RS-8 (Shimoga) isolates showed 99 per cent similarity with NCBI published sequence of RS Strain MR11 plasmid mega plasmid flagellin protein (fliC) gene, partial cds (KF031064.1) and DSM9544 flagellin (fliC) gene, partial cds (AY192724.1) respectively. Dharwad isolate showed 98 per cent similarity with Ralstonia solanacearum strain RCR-226 flagellin protein (fliC) gene, partial cds (KC834785.1). While Uttara Kannada isolate showe 97 per cent similarity with RS flagellin (fliC) gene, complete cds (AF283285.1).
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