Skip to main content

R. Borriss

Followers

21

Following

12

Co-authors

8

Public Views

In 1993, I was awarded as Professor for Bacterial Genetics at Humboldt-University Berlin. In 2000-2001, Dr. Borriss became deputy director of the Institute of Biology at Humboldt University Berlin. I became retired in 2010 and act now as collaborative scientist at Institut of Marine Biotechnology Greifswald and as adjunct professor at Chinese Agriculture University Beijing, China.

less

Mike Christenson

University of Minnesota - Twin Cities

University of California, San Diego

ICGEB-India

Rızvan İMAMOĞLU

Bartın University

ICGEB-India

Alabama State University

University of Alabama at Birmingham

University Of Jos Nigeria

Grażyna Dąbrowska

Nicolaus Copernicus University

University of Oslo

Interests

Uploads

Papers by R. Borriss

Thiazole/Oxazole-Modified Microcins: Ribosomal Templates for Complex Natural Products

Individual amino acids in the N‐terminal loop region determine the thermostability and unfolding characteristics of bacterial glucanases

Protein Science, 1996

Thermostability and unfolding behavior of the wild‐type (1,3–1,4)‐β‐glucanases from Bacillus mace... more Thermostability and unfolding behavior of the wild‐type (1,3–1,4)‐β‐glucanases from Bacillus macerans (MAC) and Bacillus amyloliquefaciens (AMY) and of two hybrid enzymes H(A12‐M)ΔF14 and H(A12‐M)ΔY13F14A were studied by spectroscopic and microcalorimetric measurements. H(A12‐M)ΔF14 is constructed by the fusion of 12 N‐terminal amino acids of AMY with amino acids 13–214 of MAC, and by deletion of F14. In H(A12‐M)ΔY13F14A, the N‐terminal region of MAC is exchanged against the AMY sequence, Y13 is deleted, and Phe 14 is exchanged against Ala. The sequence of the N‐terminal loop region from Pro 9 to amino acid 16 (or 17) is very important for the properties of the enzymes and influences the effects of Ca2+ ions on the thermostability and unfolding behavior of the enzymes. The half transition temperatures Tm are higher in the presence of Ca2+ than in Ca2+ free buffer. Furthermore, the unfolding mechanism is influenced by Ca2+. In Ca2+‐free buffer, MAC, H(A12‐M)ΔF14 and H(A12‐M)ΔY13F14A ...

Nucleic Acid Techniques in Bacterial Systematics, E. Stackebrandt, M. Goodfellow (Eds.). John Wiley & Sons, Chichester (1991), 329 S. (Ser.: Modern Microbiological Methods), 55 pound sterling. ISBN, ISBN: 0-471-92906-9

Zentralblatt für Mikrobiologie, 1993

Interactions of <em>Bacillus amyloliquefaciens</em> FZB42 with <em>Rhizoctonia solani </em>during colonization of Lettuce <em>(Lactuca Sativa) </em>roots

Minimum Information about a Biosynthetic Gene cluster

Nature chemical biology, Jan 18, 2015

BuchbesprechungGentechnologische Arbeitsmethoden, R. Hagemann (Ed.), Akademie Verlag, Berlin (1990), 233 S., 48,— DM. ISBN, ISBN: 3-05-500641-0

Zentralblatt für Mikrobiologie, 1993

The β-glucanase gene from Bacillus amyloliquefaciens shows extensive homology with that of Bacillus subtilis

Gene, 1986

The complete genome sequence of the gram-positive bacterium Bacillus subtilis

Nature, 1997

Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons

International Journal of Systematic and Evolutionary Microbiology, Sep 3, 2010

Antagonistic activity of Bacillus amyloliquefaciens subsp. amyloliquefaciens against multidrug re... more

Mapping and cloning of the gene coding for beta-glucanase from various bacilli

Molecular Genetics Microbiology and Virology (Russian version), 1985

Beta-glucanase gene from Bacillus subtilis 168 has been mapped by bacteriophage pBS1 transduction... more Beta-glucanase gene from Bacillus subtilis 168 has been mapped by bacteriophage pBS1 transduction technique between sacA and purA genes. The stimulating effect of pleiotropic mutations pap, amyB and sacUh on beta-glucanase production in Bacillus subtilis and Bacillus amyloliquefaciens has been described. Beta-glucanase gene from Bacillus amyloliquefaciens has been cloned ona Charon 4A vector. Expression of the gene in E. coli cells depended on the orientation of the cloned DNA on a pBR322 vector plasmid. Maximal enzymatic activity was registered in periplasm. Beta-glucanase gene was recloned in Bacillus subtilis cells. Bacillus subtilis strain, harbouring pBG1, produces 500 times more beta-glucanase as compared with the wild type strain of Bacillus subtilis.

beta-1,3-1,4-Glucanase in sporeforming microorganisms. II. Production of beta-glucan-hydrolases by various Bacillus species (author's transl)

Zentralblatt für Bakteriologie Parasitenkunde Infektionskrankheiten und Hygiene Zweite Naturwissenschaftliche Abteilung Mikrobiologie der Landwirtschaft der Technologie und des Umweltschutzes, 1980

Production of beta-1,3-1,4-glucan hydrolase (licheninase) was studied in 45 strains of Bacillus r... more Production of beta-1,3-1,4-glucan hydrolase (licheninase) was studied in 45 strains of Bacillus representing 17 various species using lichenin as substrate. It was found that the enzyme was produced by the strains of B. pumilus, B. subtilis, B. amyloliquefaciens, B. polymyxa, B. macerans, B. laterosporus and one strain of B. circulans. A new screening method based on application of a gel prepared from the cross-linked lichenin as an only source of carbon in the cultivation medium is presented. Using this method and methods using other cross-linked gels as carbon sources in a synthetic liquid medium, production of licheninase, amylase and cellulase by the strains tested is compared.

Buchbesprechung

Zentralblatt für Mikrobiologie

Buchbesprechung

Zentralblatt für Mikrobiologie

Influence of Ca2+ on Conformation and Stability of Three Bacterial Hybrid Glucanases

European Journal of Biochemistry, 2008

Dual role of the PhoP~ P response regulator: Bacillus amyloliquefaciens FZB45 phytase gene transcription is directed by positive and negative interactions with the …

Journal of …, 2006

Institut für Biologie, Humboldt Universität Berlin, Chausseestrasse 117, D-10115 Berlin, Germany,... more

Plantazolicin, a novel microcin B17/streptolysin S-like natural product from Bacillus amyloliquefaciens FZB42

Journal of …, 2011

Here we report on a novel thiazole/oxazole-modified microcin (TOMM) from Bacillus amyloliquefacie... more Here we report on a novel thiazole/oxazole-modified microcin (TOMM) from Bacillus amyloliquefaciens FZB42, a Gram-positive soil bacterium. This organism is well known for stimulating plant growth and biosynthesizing complex small molecules that suppress the growth of bacterial and fungal plant pathogens. Like microcin B17 and streptolysin S, the TOMM from B. amyloliquefaciens FZB42 undergoes extensive posttranslational modification to become a bioactive natural product. Our data show that the modified peptide bears a molecular mass of 1,335 Da and displays antibacterial activity toward closely related Gram-positive bacteria. A cluster of 12 genes that covers ∼10 kb is essential for the production, modification, export, and self-immunity of this natural product. We have named this compound plantazolicin (PZN), based on the association of several producing organisms with plants and the incorporation of azole heterocycles, which derive from Cys, Ser, and Thr residues of the precursor peptide.

Purification and characterization of an extracellular -glucanase fromBacillus IMET B 376

Journal of Basic Microbiology, 1981

The [beta]-glucanase gene from Bacillus amyloliquefaciens shows extensive homology with that of Bacillus subtilis

Gene, 1986

The nucleotide sequence of a 1583-bp DNA fragment containing gene bg1A for endo-beta-1,3-1,4-gluc... more The nucleotide sequence of a 1583-bp DNA fragment containing gene bg1A for endo-beta-1,3-1,4-glucanase (EC 3.2.1.73) of Bacillus amyloliquefaciens strain BE20/78, a high producer of secreted enzymes, has been determined. The gene bg1A comprises an open reading frame (ORF) of 717 bp (= 239 codons) starting with ATG at 469 up to the translation stop codon TAA at 1188. Upstream from the translation initiation codon ATG, the ribosome-binding sequence 5'-AAAAAAGGGGG-3' and two putative bglA promoters have been identified. A box of eleven AT out of twelve base pairs (bp) precedes the -35 region of promoter P1. Beyond the translation stop codon UAA, a sequence of 69 bp can be folded into a hook-like stem-loop structure which probably functions as a transcriptional terminator. The ORF region of the gene bglA reveals about 90% homology with another beta-glucanase gene, bglS of Bacillus subtilis C120 sequenced by Murphy et al. (1984). Three regions of frequent amino acid (aa) changes ...

Cloning and Expression of Bacillus β-Glucanase Genes

Gene Manipulation and Expression, 1985

Draft Genome Sequence of Bacillus atrophaeus UCMB-5137, a Plant Growth-Promoting Rhizobacterium

Genome announcements, Jan 20, 2013

Bacillus atrophaeus UCMB-5137 shows an extraordinary activity in root colonization and plant and ... more Bacillus atrophaeus UCMB-5137 shows an extraordinary activity in root colonization and plant and crop protection. Its draft genome sequence comprises 21 contigs of 4.11 Mb, harboring 4,167 coding sequences (CDS). The genome carries several genes encoding antimicrobial lipopeptides and polyketides. Multiple horizontally acquired genes of possible importance for plant colonization were also found.

Thiazole/Oxazole-Modified Microcins: Ribosomal Templates for Complex Natural Products

Individual amino acids in the N‐terminal loop region determine the thermostability and unfolding characteristics of bacterial glucanases

Protein Science, 1996

Thermostability and unfolding behavior of the wild‐type (1,3–1,4)‐β‐glucanases from Bacillus mace... more Thermostability and unfolding behavior of the wild‐type (1,3–1,4)‐β‐glucanases from Bacillus macerans (MAC) and Bacillus amyloliquefaciens (AMY) and of two hybrid enzymes H(A12‐M)ΔF14 and H(A12‐M)ΔY13F14A were studied by spectroscopic and microcalorimetric measurements. H(A12‐M)ΔF14 is constructed by the fusion of 12 N‐terminal amino acids of AMY with amino acids 13–214 of MAC, and by deletion of F14. In H(A12‐M)ΔY13F14A, the N‐terminal region of MAC is exchanged against the AMY sequence, Y13 is deleted, and Phe 14 is exchanged against Ala. The sequence of the N‐terminal loop region from Pro 9 to amino acid 16 (or 17) is very important for the properties of the enzymes and influences the effects of Ca2+ ions on the thermostability and unfolding behavior of the enzymes. The half transition temperatures Tm are higher in the presence of Ca2+ than in Ca2+ free buffer. Furthermore, the unfolding mechanism is influenced by Ca2+. In Ca2+‐free buffer, MAC, H(A12‐M)ΔF14 and H(A12‐M)ΔY13F14A ...

Nucleic Acid Techniques in Bacterial Systematics, E. Stackebrandt, M. Goodfellow (Eds.). John Wiley & Sons, Chichester (1991), 329 S. (Ser.: Modern Microbiological Methods), 55 pound sterling. ISBN, ISBN: 0-471-92906-9

Zentralblatt für Mikrobiologie, 1993

Interactions of <em>Bacillus amyloliquefaciens</em> FZB42 with <em>Rhizoctonia solani </em>during colonization of Lettuce <em>(Lactuca Sativa) </em>roots

Minimum Information about a Biosynthetic Gene cluster

Nature chemical biology, Jan 18, 2015

BuchbesprechungGentechnologische Arbeitsmethoden, R. Hagemann (Ed.), Akademie Verlag, Berlin (1990), 233 S., 48,— DM. ISBN, ISBN: 3-05-500641-0

Zentralblatt für Mikrobiologie, 1993

The β-glucanase gene from Bacillus amyloliquefaciens shows extensive homology with that of Bacillus subtilis

Gene, 1986

The complete genome sequence of the gram-positive bacterium Bacillus subtilis

Nature, 1997

Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons

International Journal of Systematic and Evolutionary Microbiology, Sep 3, 2010

Antagonistic activity of Bacillus amyloliquefaciens subsp. amyloliquefaciens against multidrug re... more

Mapping and cloning of the gene coding for beta-glucanase from various bacilli

Molecular Genetics Microbiology and Virology (Russian version), 1985

Beta-glucanase gene from Bacillus subtilis 168 has been mapped by bacteriophage pBS1 transduction... more Beta-glucanase gene from Bacillus subtilis 168 has been mapped by bacteriophage pBS1 transduction technique between sacA and purA genes. The stimulating effect of pleiotropic mutations pap, amyB and sacUh on beta-glucanase production in Bacillus subtilis and Bacillus amyloliquefaciens has been described. Beta-glucanase gene from Bacillus amyloliquefaciens has been cloned ona Charon 4A vector. Expression of the gene in E. coli cells depended on the orientation of the cloned DNA on a pBR322 vector plasmid. Maximal enzymatic activity was registered in periplasm. Beta-glucanase gene was recloned in Bacillus subtilis cells. Bacillus subtilis strain, harbouring pBG1, produces 500 times more beta-glucanase as compared with the wild type strain of Bacillus subtilis.

beta-1,3-1,4-Glucanase in sporeforming microorganisms. II. Production of beta-glucan-hydrolases by various Bacillus species (author's transl)

Zentralblatt für Bakteriologie Parasitenkunde Infektionskrankheiten und Hygiene Zweite Naturwissenschaftliche Abteilung Mikrobiologie der Landwirtschaft der Technologie und des Umweltschutzes, 1980

Production of beta-1,3-1,4-glucan hydrolase (licheninase) was studied in 45 strains of Bacillus r... more Production of beta-1,3-1,4-glucan hydrolase (licheninase) was studied in 45 strains of Bacillus representing 17 various species using lichenin as substrate. It was found that the enzyme was produced by the strains of B. pumilus, B. subtilis, B. amyloliquefaciens, B. polymyxa, B. macerans, B. laterosporus and one strain of B. circulans. A new screening method based on application of a gel prepared from the cross-linked lichenin as an only source of carbon in the cultivation medium is presented. Using this method and methods using other cross-linked gels as carbon sources in a synthetic liquid medium, production of licheninase, amylase and cellulase by the strains tested is compared.

Buchbesprechung

Zentralblatt für Mikrobiologie

Buchbesprechung

Zentralblatt für Mikrobiologie

Influence of Ca2+ on Conformation and Stability of Three Bacterial Hybrid Glucanases

European Journal of Biochemistry, 2008

Dual role of the PhoP~ P response regulator: Bacillus amyloliquefaciens FZB45 phytase gene transcription is directed by positive and negative interactions with the …

Journal of …, 2006

Institut für Biologie, Humboldt Universität Berlin, Chausseestrasse 117, D-10115 Berlin, Germany,... more

Plantazolicin, a novel microcin B17/streptolysin S-like natural product from Bacillus amyloliquefaciens FZB42

Journal of …, 2011

Here we report on a novel thiazole/oxazole-modified microcin (TOMM) from Bacillus amyloliquefacie... more Here we report on a novel thiazole/oxazole-modified microcin (TOMM) from Bacillus amyloliquefaciens FZB42, a Gram-positive soil bacterium. This organism is well known for stimulating plant growth and biosynthesizing complex small molecules that suppress the growth of bacterial and fungal plant pathogens. Like microcin B17 and streptolysin S, the TOMM from B. amyloliquefaciens FZB42 undergoes extensive posttranslational modification to become a bioactive natural product. Our data show that the modified peptide bears a molecular mass of 1,335 Da and displays antibacterial activity toward closely related Gram-positive bacteria. A cluster of 12 genes that covers ∼10 kb is essential for the production, modification, export, and self-immunity of this natural product. We have named this compound plantazolicin (PZN), based on the association of several producing organisms with plants and the incorporation of azole heterocycles, which derive from Cys, Ser, and Thr residues of the precursor peptide.

Purification and characterization of an extracellular -glucanase fromBacillus IMET B 376

Journal of Basic Microbiology, 1981

The [beta]-glucanase gene from Bacillus amyloliquefaciens shows extensive homology with that of Bacillus subtilis

Gene, 1986

The nucleotide sequence of a 1583-bp DNA fragment containing gene bg1A for endo-beta-1,3-1,4-gluc... more The nucleotide sequence of a 1583-bp DNA fragment containing gene bg1A for endo-beta-1,3-1,4-glucanase (EC 3.2.1.73) of Bacillus amyloliquefaciens strain BE20/78, a high producer of secreted enzymes, has been determined. The gene bg1A comprises an open reading frame (ORF) of 717 bp (= 239 codons) starting with ATG at 469 up to the translation stop codon TAA at 1188. Upstream from the translation initiation codon ATG, the ribosome-binding sequence 5'-AAAAAAGGGGG-3' and two putative bglA promoters have been identified. A box of eleven AT out of twelve base pairs (bp) precedes the -35 region of promoter P1. Beyond the translation stop codon UAA, a sequence of 69 bp can be folded into a hook-like stem-loop structure which probably functions as a transcriptional terminator. The ORF region of the gene bglA reveals about 90% homology with another beta-glucanase gene, bglS of Bacillus subtilis C120 sequenced by Murphy et al. (1984). Three regions of frequent amino acid (aa) changes ...

Cloning and Expression of Bacillus β-Glucanase Genes

Gene Manipulation and Expression, 1985

Draft Genome Sequence of Bacillus atrophaeus UCMB-5137, a Plant Growth-Promoting Rhizobacterium

Genome announcements, Jan 20, 2013

Bacillus atrophaeus UCMB-5137 shows an extraordinary activity in root colonization and plant and ... more Bacillus atrophaeus UCMB-5137 shows an extraordinary activity in root colonization and plant and crop protection. Its draft genome sequence comprises 21 contigs of 4.11 Mb, harboring 4,167 coding sequences (CDS). The genome carries several genes encoding antimicrobial lipopeptides and polyketides. Multiple horizontally acquired genes of possible importance for plant colonization were also found.