Papers by Qazi Parvaiz Hassan
FEMS microbiology ecology/FEMS microbiology, ecology, Mar 4, 2024
IntechOpen eBooks, Nov 9, 2022
Letters in Applied Microbiology, 2017
The study presents the isolation of rare actinobacteria from untapped sites in the Northwestern H... more The study presents the isolation of rare actinobacteria from untapped sites in the Northwestern Himalayas and their in vitro potential against Mycobacterium tuberculosis for their metabolites.The study revealed that exploring the untapped natural sources as one of the resourceful approaches for the discovery of new natural products. This study also provided strong evidence for the ability of rare and potent actinobacterial strains to produce bioactive compounds with antagonistic activity and these metabolites can be studied for inhibitory potential.
Journal of Applied Research on Medicinal and Aromatic Plants, 2019
Abstract Epimedium elatum (Morren & Decne) is a rare monotypic high altitude medicinal specie... more Abstract Epimedium elatum (Morren & Decne) is a rare monotypic high altitude medicinal species, endemic to shady forests in Northwestern Himalayas, India. Its recent chemoprofiling has revealed high concentration of Epimedin B, C & Icariin, thus matching quality standards of Herba Epimedii. DNA barcode was hitherto unreported in E.elatum. Three plastid genes (matK, rbcL & trnH-psbA) and one nuclear region gene (ITS) were partially sequenced to develop unique barcode and also investigate nucleotide diversity and phylogenetic relationship among five geographically diverse accessions of E.elatum. We used the Kimura 2-parameter (K2P) evolutionary model to calculate basic sequence statistics like conserved, variable, parsimony informative and singleton sites, including pairwise genetic distance in MEGA X software. Besides, we employed a maximum likelihood phylogenetic tree method for estimating the evolutionary divergences. The present study revealed correct blast validation of E.elatum populations with other Epimedium species. A total of 18 new sequences were obtained and submitted to NCBI Genbank via BankIT submission tool (MH615782–MH615799). Highest variable (polymorphic) sites were found in matK (269) followed by ITS (115); rbcL (18) and least in trnH-psbA (16). The evolutionary sequence divergence varied from 0.01 in rbcL/trnH-psbA; 0.09 in ITS to 0.19 in matK. Haplotype and single nucleotide polymorphism profile was rich in matK & ITS sequencing. Both primers developed a reliable and highly efficient way of determining nucleotide sequence diversity in E.elatum. ITS & matK met the ideal DNA barcode characteristics laid by Consortium of Barcode of Life and hence can be endorsed as ideal barcode markers for the identification of E.elatum in NW Himalayas, India.
South African Journal of Botany, Mar 1, 2023
South African Journal of Botany
bioRxiv (Cold Spring Harbor Laboratory), May 28, 2024
The linear genome of genus Streptomyces members has the potential to encode diverse and novel bio... more The linear genome of genus Streptomyces members has the potential to encode diverse and novel biosynthetic gene clusters of invaluable antimicrobial and therapeutic significance. The use of limited taxonomic markers makes the precise identification of these miracle microbes very challenging. In the ongoing omics era, genome sequencing and in-silico analysis of these potential antibiotic producers provide deeper insights into their taxonomy, functional capabilities, and potential for antibiotic production. Here this study presents a multifaceted approach for proper taxonomic identification and genomic and bioinformatic analysis of five bioactive Streptomyces species collected from different sampling sites in the high-altitude oligotrophic North-Western Himalaya, Kashmir, India. We used polyphasic taxonomic classification approaches, such as phylogenetic markers (16S rDNA and gyrase B), average nucleotide identity (ANI) estimation, and digital DNA-DNA hybridization (dDDH), which revealed accurate taxonomic placement of five Streptomyces species, named as, Streptomyces violarus ASQP_29, S. rhizosphaerihabitans ASQP_78, S. fulvoviolaceus ASQP_80, S. mirabilis ASQP_98, and S. thajiwasiensis ASQP_92. Amongst these, one notable finding is the discovery of a novel species proposed as Streptomyces thajiwasiensis sp. nov. ASQP_92. In addition, our study presents the first genome announcement report and analysis for S. rhizosphaerihabitans ASQP_78. Genomic annotation highlighted the presence of an exceptionally high number of poorly characterized genes and hypothetical proteins, indicating their potential for undiscovered biotechnological applications. Clusters of orthologous groups (COG) and gene ontology (GO) analysis provided insights into their varied functional roles in metabolism, signaling, information storage and secondary metabolite biosynthesis. Domainbased functional characterization further detailed their involvement in various biological processes particularly in antibiotic biosynthesis, transport, and resistance. Biosynthetic gene clusters (BGC) analysis demonstrated their diverse metabolite biosynthetic capabilities and identified both unique and conserved BGCs emphasizing the species-specific roles in bioactive metabolite production and the potential of orphan BGCs in novel drug discovery.
Analytical Chemistry Letters
Epimedium is a genus of perennial herbs in family Berberidaceae, which are eminent in Chinese eth... more Epimedium is a genus of perennial herbs in family Berberidaceae, which are eminent in Chinese ethno-pharmacology due to varied pharmaceutical properties ranging from aphrodisiac, anti-osteoporosis, anticancer, antioxidant, anti-fatigue and anti-ageing to antiviral activities. The biological activities of the plant are attributed to four major chemical constituents—icariin, epimedin A, epimedin B and Epimedium C (ABCI). Epimedium elatum (Morr & Decne), inhabiting Northwestern Himalayas of India, is a unique and rare perennial medicinal herb which has not been researched and exploited much in terms of phytochemistry and molecular aspects.
Natural Product Research
In the present study, we report herein the isolation of cadinane-type sesquiterpenoid, tatarinowi... more In the present study, we report herein the isolation of cadinane-type sesquiterpenoid, tatarinowin A (ACH-6), and pentadecanoic acid (ACH-8) from petroleum ether extract of rhizome of Acorus calamus L. (Acoraceae) along with 6 other known compounds in this species. It is pertinent to mention here that this is the first report to stain these compounds in which dereplication approach based on GC-MS was applied to target unknown compounds ACH-6 and ACH-8 in A. calamus L. Derelpication approaches based on GC-MS is very useful technique in the area of drug discovery and have eminence potential to identify known and unknown compounds present in extracts of medicinal important plants. This technique can expedite the process of purification of unknown compounds from different matrixes. The isolated compounds were identified with the help of inbuilt library search which reveals the presence of 17 known and 4 unknown compounds. Further, the structure elucidation of all isolated compounds was done using spectroscopy techniques. Also, the structure of ACH-6 was further confirmed by using the single-crystal X-ray diffraction technique.
Current Botany, 2017
They are mostly perennial species with heart shaped leaves and majority of them have 4-parted "sp... more They are mostly perennial species with heart shaped leaves and majority of them have 4-parted "spider-like" flowers in spring season (Ma et al., 2011). They are distributed in North Temperate Zones in China, Japan, Korea, Europe and some African countries (Zhang et al., 2016). Medicinally, they are known as Herba Epimedii, a famous botanical supplement prepared from 5 Epimedium species, viz., Epimedium brevicornum, Epimedium sagittatum, Epimedium koreanum, Epimedium pubescens, and Epimedium wushanense (Ma et al., 2011). Epimedin-A, Epimedin-B, Epimedin-C, and Icariin are the major chemical constituents in almost all Epimedium species (Chen et al., 2015).They are known to possess a number of pharmacological actions such as aphrodisiac (phosphodiesterase-5 inhibition), antiosteoporosis, antioxidation, antitumor, and antiaging activities (Ma et al., 2011). E. elatum (Morren and Decne) is a sparsely branched, low growing, perennial, and deciduous medicinal herb, which reaches a height of about 100 cm in shady habitats in Kashmir Himalayas (www.srgc.net). According to recent reports, E.
South African Journal of Botany
Applied Microbiology and Biotechnology
Rice (Oryza sativa L.) is one of the most important staple foods consumed in many countries of th... more Rice (Oryza sativa L.) is one of the most important staple foods consumed in many countries of the world. It is mostly consumed in developing countries where different chemical fertilizers are used to improve the productivity of the crop plant. In the present study, endophytic actinomycetes isolated from Rumex dentatus were identified morphologically and by scanning electron microscopy. Butyl isobutyl phthalate (BIBP) was isolated from the root endophyte Streptomyces sp. JR9 using column chromatography and HPLC methods. The compound was tested for its effect on rice seed germination. BIBP, extracts, and isolates were evaluated for their plant growth effect on rice in a growth chamber. Isolates were also screened in vitro for phosphate solubilization activity and enzyme production. Indole-3-acetic acid (IAA) and BIBP produced in extracts were quantified and detected using high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) methods, respectively. BIBP was found to increase the germination of rice seeds by 6 to 12% in treated samples and displayed potent effect at lowest concentration (0.437 µM). Both the compound and the extract depicted significant increases in almost all growth parameters at lowest concentration of 0.125 µg/mL and 62.5 µg/mL, respectively. BIBP also increased significantly shoot length, fresh root, fresh shoot, and dried shoot weight at high concentrations and was more potent than the standard phytohormone IAA. HPLC quantification showed 7.952 µg/mg and 0.371 µg/mg of IAA in extracts of Streptomyces sp. JR9 and the stem endophyte Streptomyces sp. KS3, respectively. IAA containing extract of JR9 increased significantly most growth parameters at lowest concentration (125 µg/mL). The extract of KS3 depicted significant increases in almost all growth parameters at high concentration (500 µg/mL). Our investigation showed that streptomycetes isolated from R. dentatus and BIBP are potent growth promoting agents and can be used in agriculture as bio-fertilizer to improve the growth and productivity of rice. KEY POINTS: • Butyl isobutyl phthalate (BIBP) isolated from endophytic Streptomyces sp. JR9 is a potent rice seed germination activator and promotes significantly the growth of rice • Isolated endophytes showed the ability to produce enzymes and phytohormone IAA • Isolates enhanced significantly the growth of rice.
The Journal of Phytopharmacology, 2017
Emodin (1,3,8-trihydroxy-6-methylanthraquinone) is a Chinese herbal anthraquinone that exhibits n... more Emodin (1,3,8-trihydroxy-6-methylanthraquinone) is a Chinese herbal anthraquinone that exhibits numerous biological activities, such as antitumor, antibacterial, antiinflammatory, and immunosuppressive. From an endophytic fungus, a close relative of Polyporales sp., found in association with Rheum emodi, Wall. ex Meissn a compound (Rz) was isolated and characterizedby different spectroscopic techniques (1H-NMR, 13CNMR, 2D-NMR, and HRMS). The compound (Rz) displayed a range of cytotoxicities against different human cancer cell lines like THP-1(Leukemia), A549 (Lung), NCI-H322 (lung) and Colo-205(colon) at a concentration of 70 and 100 µM. The compound had strong anticancer activity by arresting the cell cycle at G1 and G2/M phase and loss of mitochondrial membrane potential in A-549 lung cancer cells in concentration dependent manner. The study suggests that emodin induced anticancer effects may have novel therapeutic applications for the treatment of lung cancer.
Journal of Plant Biochemistry and Biotechnology, 2019
The phytochemistry of Hypericum perforatum (St. John's wort) has been elaborated extensively owin... more The phytochemistry of Hypericum perforatum (St. John's wort) has been elaborated extensively owing to its immense application in medicinal chemistry. Illustrated pharmacological activities like antidepressant, antiviral, and antibacterial effects demonstrated its substantiation for numerous of the conventional purposes reported for St John's wort. St. John's wort is herbal remedy extensively used in mild to moderate depression. Most of pharmacological activities were assigned to presence of photosensitive naphthodianthrone; hypericin and other allied flavonoid constituents. Escalating demands of hyperforin as antidepressant added more lure to H. perforatum L. The crude extracts of H. perforatum containing phloroglucinols were used for free radical scavenging and against DNA damage. To meet increasing demands of this drug, researchers need to tailor out the biosynthetic pathways to improve secondary metabolite. This necessitated advancement in biotechnological intervention to improve phytochemical potential of this growing herb. This review will brief out ecology, chemistry and phytochemical efficacy with respective phytoconstituents. Further, review will emphasize biotechnological interventions including both conventional and modern contrivances that have been implemented to augment the glory of this species.
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Papers by Qazi Parvaiz Hassan