Papers by Przemysław Prządka
Applied Sciences
Electrochemotherapy (ECT) with bleomycin has been effectively used in recent years to treat vario... more Electrochemotherapy (ECT) with bleomycin has been effectively used in recent years to treat various skin tumors. Microsecond electric pulses significantly improve bleomycin (BLM) delivery and its anticancer potential. Up to now, we can determine electric field distribution in the targeted tissue, however, the distribution of the injected drug is still not well known. In this study, we propose the combination of indocyanine green (ICG) with bleomycin as a practical approach for ECT, enabling drug distribution control and detection. Normal skeletal muscle (L6) and fibrosarcoma (WEHI-164) cells were used for the viability evaluation by MTT assay after 24 and 72 h. Cells were exposed to the ESOPE protocol alone and in combination with drugs. Additionally, visualization of the uptake of ICG and ICG + BLM supported by electroporation was performed by confocal microscopy. The mast cell tumor (MCTs) was diagnosed in the feline case. The mixture of ICG + BLM was injected into the tumor, and ...
Animals
All the surgical approaches described to date for the removal of distal urethral tumors have some... more All the surgical approaches described to date for the removal of distal urethral tumors have some technical difficulties that make these tumors difficult to treat. The article presents for the first time the treatment results of three female dogs, diagnosed with transitional cell carcinomas of the distal urethra, operated with a newly developed hybrid surgical method—a combination of laparoscopy and open surgery. This technique uses vulvovaginectomy, combined with resection of the distal urethra and prepubic urethrostomy. All of the procedures were possible to perform, without the need to carry out a laparotomy conversion. Histopathology revealed transitional cell carcinoma in all cases, with a margin of healthy tissues maintained in two out of three cases, which meant reoperation of the urethrostomy site in the remaining one case. The mean survival time was nine months. Among minor complications, recurrent cystitis was found. After the first surgery, all dogs retained full control ...
Journal of Small Animal Practice
Pakistan Veterinary Journal, 2019
Neoplastic testicular lesions are diagnosed increasingly frequently in dogs and humans. In rats, ... more Neoplastic testicular lesions are diagnosed increasingly frequently in dogs and humans. In rats, spontaneous testicular tumours, particularly Leydig cell tumours, are very rare. The aim of the study was to carry out a histological and immunohistochemical analysis of a Leydig cell tumour in a rat as well as to present the similarities and differences in the expression of the proteins used to diagnose this type of tumour in dogs and men. Following the histopathological and immunohistochemical analysis (including antibodies against vimentin, calretinin, inhibin-α, β catenin and E-cadherin), significant similarities were found in the level of expression of the studied cell markers of the rat, dog and male leydigoma. This indicates their usefulness as diagnostic markers of testicle tumours both in humans and animals.
Experimental and Therapeutic Medicine
While hyperthermic intraperitoneal applications have demonstrated high efficacy in treating perit... more While hyperthermic intraperitoneal applications have demonstrated high efficacy in treating peritoneal metastases (PM), these applications are limited to temperatures of 41-43˚C to prevent a harmful increase in core temperature. However, since gaseous substances display low specific heat capacities, gas-based hyperthermia could potentially increase surface temperatures without affecting the body's core temperature. To the best of our knowledge, the present study is the first to explore the in vivo feasibility of gas-based hyperthermia via spatial and time-based distribution. In the present study, a temperature-isolated, abdominal box model was created with fresh peritoneal tissue exposed to continuous high-volume airflow temperatures ranging between 47 and 69˚C. Heat conduction within the peritoneal tissues was measured using temperature microsensors. Temperature build-up at different time points during the procedure was calculated and the safest option to perform gas-based intraperitoneal hyperthermia beyond 43˚C was identified using an in vivo swine model. In subsequent experiments, viability and cytotoxicity of HT-29 colon cancer cells were measured following short-term hyperthermia. The present study demonstrated that the application of gas-based intraperitoneal hyperthermia with temperatures up to 50˚C is possible without increasing the core temperature to harmful levels. Gas-based intraperitoneal hyperthermia can induce a histological reaction on the peritoneal surface, and it can also result in decreased viability and increased cytotoxicity of HT-29 cells. The concept of extreme hyperthermia may be of great clinical importance as it could significantly increase local cytotoxicity in PM without increasing the body's core temperature. Further studies are required to investigate the benefits, as well as the restrictions, of this novel concept.
Frontiers in Oncology
Background43°Celsius (C) is currently the highest temperature used in the treatment of peritoneal... more Background43°Celsius (C) is currently the highest temperature used in the treatment of peritoneal metastasis (PM). Despite sufficient data on water- based hyperthermic solutions in PM treatment, there is currently no information on gas-based hyperthermia extending beyond 43°C. This study is the first to provide in-vivo data on different organ systems during and after intraperitoneal gas-based hyperthermia beyond 43°C. The aim of this study is to explore in-vivo feasibility, safety, and efficacy of this novel concept from a biological perspective.MethodsFor this study, three swine were subjected to laparoscopy and subsequent gas-based intraperitoneal hyperthermia at 48°, 49° and 50°C under a high-flow air stream. Intraoperative data from multiple temperature sensors were analysed. Additionally, intraoperative anaesthesiologic and gasometrical data was analysed. Postoperatively, swine were monitored for one week and laboratory work-up was performed on postoperative days 1, 3 and 7.Res...
Frontiers in Oncology
BackgroundWhile hyperthermic intraperitoneal (i.p) applications are highly efficient in treating ... more BackgroundWhile hyperthermic intraperitoneal (i.p) applications are highly efficient in treating peritoneal metastases (PM), they are currently limited to temperatures of 41 – 43° Celsius (C). First data on gas-based i.p. hyperthermia is promising, as this novel method allows a significant temperature rise in superficial peritoneal layers without increasing core temperatures. Until now, key mechanisms of this novel tool, e.g. thermodynamic energy transfer, have not been investigated. This study aims to explore the volume of thermodynamic energy transfer during gas-based i.p. hyperthermia at 48-50°C and its peritoneal effects.MethodsFor this study, three swine were subjected to gas-based i.p. hyperthermia at varying temperatures (48°, 49° and 50°C) in a diagnostic laparoscopy setting with a high-flow air stream. Temperatures of the i.p. cavity, in- and outflow airstream at the trocar were measured and the thermodynamic energy transfer was calculated. Tissue samples were collected on ...
Frontiers in Oncology
BackgroundPeritoneal metastasis (PM) is an ongoing challenge in surgical oncology. Current therap... more BackgroundPeritoneal metastasis (PM) is an ongoing challenge in surgical oncology. Current therapeutic options, including intravenous and intraperitoneal (i.p.) chemotherapies display limited clinical efficacy, resulting in an overall poor prognosis in affected patients. Combined hyperthermia and dehydration induced by a high-flow, gas-based i.p. hyperthermic procedure could be a novel approach in PM treatment. Our study is the first to evaluate the therapeutic potential of i.p. dehydration, hyperthermia, as well as the combination of both mechanisms in an in-vivo setting.MethodsFor this study, three swine were subjected to diagnostic laparoscopy under a high-flow air stream at 48°, 49° and 50°Celsius (C). Hygrometry of the in- and outflow airstream was measured to calculate surface evaporation and i.p. dehydration. To analyze the effects of this concept, in vitro colon cancer cells (HT-29) were treated with hyperthermia and dehydration. Cytotoxicity and cell viability were measured...
Veterinary and Comparative Oncology, 2022
Tumours of the distal urethra in female dogs are often difficult to treat, and the surgical metho... more Tumours of the distal urethra in female dogs are often difficult to treat, and the surgical methods described thus far have technical limitations. This study aimed to present a novel approach to the surgical treatment of distal urethral tumours. This study used dog cadavers to evaluate the technical feasibility of surgically removing neoplastic lesions in the distal urethra and compared surgical outcomes of open surgery with those of hybrid surgery (combination of laparoscopy and open surgery). Open intact, open spayed, hybrid intact, and hybrid spayed dog cadaver groups underwent surgery (n = 6 per group). The novel surgical method was based on vulvovaginectomy (ovariohysterectomy in intact dogs), resection of the distal part of the urethra, and pre-pubic urethrostomy. Outcomes of interest included technical feasibility of each procedure, using both surgical techniques, wound length, time required to complete the procedure, and the incidence of intraoperative ureter and rectum injuries. Surgical technique and reproductive status affected operating time. Technique choice affected wound length; the surgical wound was longer in the open group than in the hybrid group. Macroscopic evaluation of the rectum did not reveal any damage to the wall. There was no evidence of ureter leakage or obstruction in any case. The present findings suggest that both open and hybrid surgery can be used to treat distal urethral tumours.
Polish journal of veterinary sciences, 2019
Novel tendinopathy treatment protocols should be assessed for safety. The goal of this work was t... more Novel tendinopathy treatment protocols should be assessed for safety. The goal of this work was to compare differences in selected systemic inflammatory marker concentrations after two treatment protocols for collagenase induced Achilles tendinopathy in sheep. 14 sheep (aged 5 and 6 years, Polish Mountain Sheep breed, weight 60-70kg) were injected with bacterial collagenase type 1A-S (Clostridium histolyticum, C-5894, Sigma Aldrich, Poznań, Poland) bilaterally to Achilles tendons. Subsequently, the animals were injected with Platelet Rich Plasma (7 sheep) or Adipose Derived Stem Cells (7 sheep) to induced tendinopathy foci. Left limbs of all sheep were additionally treated with Radial Pressure Wave Therapy (RPWT) focused above the tendinopathy origins. Treatment progress was controlled by ultrasound scans, and tendon samples were taken on the 126th day of the experiment. Serum Amyloid A (SAA) concentration showed mild elevation before the experiment (2 sheep from group I, 4 sheep fr...
Polish journal of veterinary sciences, 2019
Tendinopathy treatment poses a current challenge for sport medicine due to unique physiology and ... more Tendinopathy treatment poses a current challenge for sport medicine due to unique physiology and biomechanics of tendons. The goal of this work was to compare the efficacy of the addition of the radial pressure wave therapy (RPWT) treatment to injection of autologous Adipose Derived Stem Cells (ADSCs) or Platelet Rich Plasma (PRP) in the therapeutic procedure for collagenase induced Achilles tendinopathy in sheep. 14 sheep (aged 5 and 6 years, Polish Mountain Sheep breed, weight 60-70 kg) were injected bacterial collagenase type 1A-S (Clostridium histolyticum, C-5894, Sigma Aldrich, Poznań, Poland) bilaterally to Achilles tendons. Subsequently, the animals were injected with PRP (7 sheep) or ADSCs (7 sheep) to previously induced tendinopathy foci. Left limbs of all the animals were additionally treated with RPWT focused above the tendinopathy origins. Treatment progress was controlled by ultrasound scans, and tendon samples were taken on the 126th day of the experiment. Tendon sampl...
Polish journal of veterinary sciences, 2020
The use of hyperbaric oxygen therapy (HBOT) is an accepted method of supporting wound healing in ... more The use of hyperbaric oxygen therapy (HBOT) is an accepted method of supporting wound healing in human medicine. Yet, because of the risk of complications associated with HOBT, a safer modification of the therapy, known as L-HOBT (lower pressure and lower oxygen concentration), is increasingly used nowadays. Therefore, due to the lack of literature reports regarding the clinical use of L-HBOT in animals, the authors decided to present the results of L-HBOT supportive treatment of postoperative wounds after hemimastectomy compared to classic treatment. The study group included 12 bitches divided into two groups: group A (assisted L-HBOT) and group B (classic treatment). In addition to conventional treatment, the supportive therapy included 1.5 hours of hyperbaric chamber therapy for 5 consecutive days, starting 24 hours after surgery (1500hPa pressure and 26% oxygen concentration in the chamber). The patients were followed and evaluated throughout the course of the treatment by two i...
Biomolecules, 2021
Regenerative medicine is a dynamically developing field of human and veterinary medicine. The ani... more Regenerative medicine is a dynamically developing field of human and veterinary medicine. The animal model was most commonly used for mesenchymal stem cells (MSCs) treatment in experimental and preclinical studies with a satisfactory therapeutic effect. Year by year, the need for alternative treatments in veterinary medicine is increasing, and other applications for promising MSCs and their biological derivatives are constantly being sought. There is also an increase in demand for other methods of treating disease states, of which the classical treatment methods did not bring the desired results. Cell therapy can be a realistic option for treating human and animal diseases in the near future and therefore additional research is needed to optimize cell origins, numbers, or application methods in order to standardize the treatment process and assess its effects. The aim of the following work was to summarize available knowledge about stem cells in veterinary medicine and their possibl...
Reproduction in Domestic Animals, 2020
Light-based technologies are applied in various fields of medicine: for example optical diagnosti... more Light-based technologies are applied in various fields of medicine: for example optical diagnostics, light-activated therapy and surgery. Although light-based surgical procedures had hardly been a novelty, the revolutionizing moment for surgery came with the first use of light to cut tissue. Nowadays, surgical lasers are routinely used across numerous medical specialties, including gynaecology and urology. They are a part of the surgical treatment of benign prostatic hyperplasia, prostate carcinoma, penis carcinoma, genital skin lesions and orchidectomy. While in human urology lasers How to cite this article: Tunikowska J, Prządka P, Kiełbowicz Z. Lasers in the surgical treatment of canine prostatic neoplasia and selected tumours of the male reproductive system.
Journal of Comparative Pathology, 2019
Introduction: Meibomian gland tumours in dogs are the most often observed proliferative lesions i... more Introduction: Meibomian gland tumours in dogs are the most often observed proliferative lesions in the eyelids. Often, the histological boundary between adenocarcinoma and adenoma is poorly defined. In addition, the accompanying inflammation makes the diagnosis more difficult. The aim of this study was to investigate cell markers that can be used to distinguish these tumours and to evaluate the intensity of inflammatory infiltration. Materials and Methods: Thirty-four adenomas and 47 adenocarcinomas verified with the WHO guidelines were used in this study. Immunohistochemistry was performed using antibodies specific for Ki67, von Willebrand factor and CK20. Additionally, the intensity of inflammatory infiltration in individual tumour groups was assessed. Results: Ki67 expression in adenomas was scored + in 30% of cases, ++ in 5% of cases and in other cases was not present. In adenocarcinomas, the expression levels of Ki67 were 15% (+++), 60% (++), 20% (+) and 5% (no expression). The mean vascular density in adenomas was 4 and in adenocarcinomas 8. CK20 expression in adenomas was not observed, in contrast to adenocarcinomas where it was +++ in 45% of cases, ++ in 50% and + in 5%. It was noticed that inflammatory infiltration was almost absent in adenomas and in adenocarcinomas it was very intense or moderate. Conclusions: Expression of selected cell markers in the examined tumours can lead to more precise diagnosis, especially in tumours with low mitotic activity or during the initial stages of tumour development. In these cases, the evaluation of CK20 is particularly useful.
Materials, 2021
Implantation of composite scaffolds could be potentially associated with the risk of hemostatic d... more Implantation of composite scaffolds could be potentially associated with the risk of hemostatic disturbances in a recipient. However, there is a lack of information on possible alterations in clotting mechanisms resulting from such a procedure. The aim of the present work was to investigate changes in hemostatic parameters in sheep implanted with a scaffold composed of poly(ε-caprolactone) and hydroxyapatite and tricalcium phosphate (9:4.5:4.5), settled previously with mesenchymal stem cells stimulated by fibroblast growth factor-2 and bone morphogenetic protein-2. Nine Merino sheep were examined for 7 days, and measurements of clotting times (PT, aPTT), activities of antithrombin, protein C and clotting factors II-XII, and concentrations of fibrinogen and D-dimer were carried out before and 1 h, 24 h, 3 days and 7 days after scaffold implantation. The introduction of scaffold initially resulted in a slowdown of the clotting processes (most evident 24 h after surgery); PT and aPTT i...
Medycyna Weterynaryjna, 2016
Platelet-rich plasma (PRP) is an autogenic source of many growth factors that are a part of the h... more Platelet-rich plasma (PRP) is an autogenic source of many growth factors that are a part of the healing process and tissue regeneration, which is the cause of its growing popularity in regenerative medicine. In this paper, we present a definition and characteristics of PRP, as well as ways of extracting it. We also present the issue of thrombocyte activation and the impact of thrombocytes on PRP. We explain how to obtain the optimal density of platelets in PRP, as well as its influence on tissues. Additionally, we describe some of the applications of PRP in experimental and clinical work.
BMC Veterinary Research
Background This paper presents the first described case of laparoscopy-assisted prepubic urethros... more Background This paper presents the first described case of laparoscopy-assisted prepubic urethrostomy and laparoscopic resection of a tumor of the distal part of the urethra in a female dog as a palliative treatment. Case presentation An intact, 11 -year-old, mixed breed female dog, weighing 15 kg, was admitted with signs of urinary obstruction and difficulty with catheterization. Vaginal, rectal, and endoscopic examinations revealed a firm mass in the pelvic cavity at the level of the pelvic urethra. Ultrasound and computed tomography examination showed enlargement of the urethral wall (5.5 cm width and 3 cm thick), which was significantly restricting the patency of the urethra. The lesion affected only the distal part of the urethra without the presence of local or distant metastatic changes. The affected portion of the urethra was laparoscopically removed while performing pre-pubic urethrostomy with laparoscopy. The patient regained full consciousness immediately after the end of...
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Papers by Przemysław Prządka