Papers by Pierluigi Mauri
Molybdenum and iron mutually impact their homeostasis in cucumber (Cucumis sativus) plants
New Phytologist, 2016
Molybdenum (Mo) and iron (Fe) are essential micronutrients required for crucial enzyme activities... more Molybdenum (Mo) and iron (Fe) are essential micronutrients required for crucial enzyme activities in plant metabolism. Here we investigated the existence of a mutual control of Mo and Fe homeostasis in cucumber (Cucumis sativus). Plants were grown under single or combined Mo and Fe starvation. Physiological parameters were measured, the ionomes of tissues and the ionomes and proteomes of root mitochondria were profiled, and the activities of molybdo-enzymes and the synthesis of molybdenum cofactor (Moco) were evaluated. Fe and Mo were found to affect each other's total uptake and distribution within tissues and at the mitochondrial level, with Fe nutritional status dominating over Mo homeostasis and affecting Mo availability for molybdo-enzymes in the form of Moco. Fe starvation triggered Moco biosynthesis and affected the molybdo-enzymes, with its main impact on nitrate reductase and xanthine dehydrogenase, both being involved in nitrogen assimilation and mobilization, and on the mitochondrial amidoxime reducing component. These results, together with the identification of > 100 proteins differentially expressed in root mitochondria, highlight the central role of mitochondria in the coordination of Fe and Mo homeostasis and allow us to propose the first model of the molecular interactions connecting Mo and Fe homeostasis.
Eur Mass Spectrom, 2000
The 1 : 1 inclusion complex of β-cyclodextrin (βCD) with 5-methoxytryptamine (5MTA) hydrochloride... more The 1 : 1 inclusion complex of β-cyclodextrin (βCD) with 5-methoxytryptamine (5MTA) hydrochloride was studied by mass spectrometry (MS) and tandem-MS with fast atom bombardment (FAB), electrospray (ES) and ionspray (IS) ionisation and triple quadrupole or ion trap analysers. A protonated 1 : 1 βCD/MTA gaseous association was always observed; the protonated species of deaminated 5MTA and of some typical βCD fragments were obtained, in addition to the expected protonated 5MTA, as tandem-MS dissociation products. A reaction pattern starting from the deamination of protonated 5MTA, as a recently described high pressure chemical ionisation or ES process of primary amines, was suggested, which accounts for the formation of excited protonated βCD and its fragmentation by tandem-MS, even under unimolecular conditions.
Nature Communications, Apr 16, 2014
The role of protein and peptide separation before mass spectrometry analysis in clinical proteomics
Journal of chromatography. A, Jan 13, 2015
The purpose of clinical proteomics is to characterise protein profiles of a plethora of diseases ... more The purpose of clinical proteomics is to characterise protein profiles of a plethora of diseases with the aim of finding specific biomarkers. These are particularly valuable for early diagnosis, and represent key molecules suitable to elucidate pathogenic mechanisms. Samples deriving from patients (i.e. blood, urine, cerebrospinal fluid, biopsies) are the sources for clinical proteomics. Due to the complexity of the extracted samples their direct analysis is unachievable. Any analytical clinical proteomics study should start with the choice of the optimal combination of strategies with respect to both sample preparations and MS approaches. Protein or peptide fractionation (off-line or on-line) is essential to reduce complexity of biological samples and to achieve the most complete and reproducible analysis. The aim of this review is to introduce the readers to a functional range of strategies to help scientists in their proteomics set up. In particular, the separation approaches of ...
Reversed-phase high-performance liquid chromatographic method for the analysis of biflavones in Ginkgo biloba L. extracts
High-performance liquid chromatographic assay for hexokinase
Journal of Chromatography A, 1987
Science of Friction–Adhesive Joints
Advanced Structured Materials, 2010
This chapter addresses the fundamental properties of hybrid friction–adhesive joints which combin... more This chapter addresses the fundamental properties of hybrid friction–adhesive joints which combine any form of mechanical tightening (stimulus for friction forces) with anaerobic adhesives. By filling the voids around the microareas of true metal-to-metal contact between the mating parts, anaerobic adhesives allow the full area involved by the engagement to be usefully exploited. Advantages ranging from sealing action, fretting suppression, noise reduction and enhanced strength derive from this combination. The focus of the chapter is on predicting the mechanical strength of these joints. The literature covering the static and the fatigue strength is reviewed showing that proper choice of the adhesive can increase the overall strength of the joint well above the strength of the purely mechanical joint based on friction only. Simple equations are also provided for the strength calculation of practical engineering assemblies.
High performance liquid chromatography/electrospray mass spectrometry of Hypericum perforatum extracts
Rapid Communications in Mass Spectrometry, 2000
![Research paper thumbnail of CXCL12 and [N33A]CXCL12 in 5637 and HeLa Cells: Regulating HER1 Phosphorylation via Calmodulin/Calcineurin](https://attachments.academia-assets.com/53122968/thumbnails/1.jpg)
PLoS ONE, 2012
In the human neoplastic cell lines 5637 and HeLa, recombinant CXCL12 elicited, as expected, downs... more In the human neoplastic cell lines 5637 and HeLa, recombinant CXCL12 elicited, as expected, downstream signals via both G-protein-dependent and b-arrestin-dependent pathways responsible for inducing a rapid and a late wave, respectively, of ERK1/2 phosphorylation. In contrast, the structural variant [N33A]CXCL12 triggered no b-arrestin-dependent phosphorylation of ERK1/2, and signaled via G protein-dependent pathways alone. Both CXCL12 and [N33A]CXCL12, however, generated signals that transinhibited HER1 phosphorylation via intracellular pathways. 1) Prestimulation of CXCR4/HER1positive 5637 or HeLa cells with CXCL12 modified the HB-EGF-dependent activation of HER1 by delaying the peak phosphorylation of tyrosine 1068 or 1173. 2) Prestimulation with the synthetic variant [N33A]CXCL12, while preserving CXCR4-related chemotaxis and CXCR4 internalization, abolished HER1 phosphorylation. 3) In cells knockdown of b-arrestin 2, CXCL12 induced a full inhibition of HER1 like [N33A]CXCL12 in non-silenced cells. 4) HER1 phosphorylation was restored as usual by inhibiting PCK, calmodulin or calcineurin, whereas the inhibition of CaMKII had no discernable effect. We conclude that both recombinant CXCL12 and its structural variant [N33A]CXCL12 may transinhibit HER1 via G-proteins/ calmodulin/calcineurin, but [N33A]CXCL12 does not activate b-arrestin-dependent ERK1/2 phosphorylation and retains a stronger inhibitory effect. Therefore, we demonstrated that CXCL12 may influence the magnitude and the persistence of signaling downstream of HER1 in turn involved in the proliferative potential of numerous epithelial cancer. In addition, we recognized that [N33A]CXCL12 activates preferentially G-protein-dependent pathways and is an inhibitor of HER1. Citation: Rigo A, Gottardi M, Damiani E, Bonifacio M, Ferrarini I, et al. (2012) CXCL12 and [N33A]CXCL12 in 5637 and HeLa Cells: Regulating HER1 Phosphorylation via Calmodulin/Calcineurin. PLoS ONE 7(4): e34432.
Nature Communications, 2014
Molecular Cancer, 2010
Background: Increased numbers of tumour-associated macrophages correlate with shortened survival ... more Background: Increased numbers of tumour-associated macrophages correlate with shortened survival in some cancers. The molecular bases of this correlation are not thoroughly understood. Events triggered by CXCL12 may play a part, as CXCL12 drives the migration of both CXCR4-positive cancer cells and macrophages and may promote a molecular crosstalk between them. Results: Samples of HER1-positive colon cancer metastases in liver, a tissue with high expression of CXCL12, were analysed by immunohistochemistry. In all of the patient biopsies, CD68-positive tumour-associated macrophages presented a mixed CXCL10 (M1)/CD163 (M2) pattern, expressed CXCR4, GM-CSF and HB-EGF, and some stained positive for CXCL12. Cancer cells stained positive for CXCR4, CXCL12, HER1, HER4 and GM-CSF. Regulatory interactions among these proteins were validated via experiments in vitro involving crosstalk between human mononuclear phagocytes and the cell lines DLD-1 (human colon adenocarcinoma) and HeLa (human cervical carcinoma), which express the above-mentioned ligand/receptor repertoire. CXCL12 induced mononuclear phagocytes to release HB-EGF, which activated HER1 and triggered anti-apoptotic and proliferative signals in cancer cells. The cancer cells then proliferated and released GM-CSF, which in turn activated mononuclear phagocytes and induced them to release more HB-EGF. Blockade of GM-CSF with neutralising antibodies or siRNA suppressed this loop.
Journal of Natural Products, 2012
Examination of the metabolites produced by an Actinospica strain led to the identification of 6-h... more Examination of the metabolites produced by an Actinospica strain led to the identification of 6-hydroxychrolactomycin (compound 1), which is produced along with minor amounts of chrolactomycin (compound 2). The structure of 1 was established on the basis of extensive spectroscopic analysis, including one-and two-dimensional NMR. Compound 1 showed antimicrobial activity against Gram-positive bacteria, although it was generally less active than 2.
High-performance liquid chromatographic method for the determination of the esterase activity of subtilisin and kallikrein
Journal of Chromatography A, 1988
Human Psychopharmacology: Clinical and Experimental, 2007
Recent data suggest that the complexation of standardised Ginkgo biloba extract (GBE) with soy-de... more Recent data suggest that the complexation of standardised Ginkgo biloba extract (GBE) with soy-derived phospholipids enhances the bio-availablity of GBE's active components. The current study therefore aimed to assess the comparative cognitive and mood effects of a low dose of GBE and products complexing the same extract with either phosphatidylserine or phosphatidylcholine.
European Journal of Mass Spectrometry, 2000
The 1 : 1 inclusion complex of β-cyclodextrin (βCD) with 5-methoxytryptamine (5MTA) hydrochloride... more The 1 : 1 inclusion complex of β-cyclodextrin (βCD) with 5-methoxytryptamine (5MTA) hydrochloride was studied by mass spectrometry (MS) and tandem-MS with fast atom bombardment (FAB), electrospray (ES) and ionspray (IS) ionisation and triple quadrupole or ion trap analysers. A protonated 1 : 1 βCD/MTA gaseous association was always observed; the protonated species of deaminated 5MTA and of some typical βCD fragments were obtained, in addition to the expected protonated 5MTA, as tandem-MS dissociation products. A reaction pattern starting from the deamination of protonated 5MTA, as a recently described high pressure chemical ionisation or ES process of primary amines, was suggested, which accounts for the formation of excited protonated βCD and its fragmentation by tandem-MS, even under unimolecular conditions.
Applied Biochemistry and Biotechnology, 1994
A rapid and integrated procedure was developed for the preparation of small DNA restriction fragm... more A rapid and integrated procedure was developed for the preparation of small DNA restriction fragments (_ 1000 bp) starting from a large cosmid (35,000 bp) containing exogenous DNA.
Analytical and Bioanalytical Chemistry, 2008
Human body fluids have been rediscovered in the post-genomic era as a great source of biological ... more Human body fluids have been rediscovered in the post-genomic era as a great source of biological markers and perhaps as source of potential biomarkers of disease. Recently, it has been found that not only proteins but also peptides and their modifications can be indicators of early pathogenic processes. This paper reports the identification of free phosphopeptides in human fluids using an improved IMAC strategy coupled to iterative mass spectrometrybased scanning techniques (neutral loss, precursor ion, multiple reaction monitoring). Many peptides were detected in the enriched extract samples when submitted to the MS-integrated strategy, whereas they were not detected in the initial extract samples. The combination of the IMACmodified protocol with selective "precursor ion" and constant "neutral loss" triple quadrupole scan modes confers a high sensitivity on the analysis, allowing rapid phosphopeptide identification and characterization, even at low concentrations. To the best of our knowledge this work represents the first report exclusively focused on the detection of free phosphorylated peptides in biological fluids.
Galectin-3: an early predictive biomarker of modulation of airway remodeling in patients with severe asthma treated with omalizumab for 36 months
Clinical and translational allergy, 2017
Bronchial asthma is a heterogeneous disease characterized by three cardinal features: chronic inf... more Bronchial asthma is a heterogeneous disease characterized by three cardinal features: chronic inflammation, variable airflow obstruction, and airway hyperresponsiveness. Asthma has traditionally been defined using nonspecific clinical and physiologic variables that encompass multiple phenotypes and are treated with nonspecific anti-inflammatory therapies. Based on the modulation of airway remodeling after 12 months of anti-immunoglobulin E (IgE) treatment, we identified two phenotypes (omalizumab responder, OR; and non-omalizumab responder, NOR) and performed morphometric analysis of bronchial biopsy specimens. We also found that these two phenotypes were correlated with the presence/absence of galectin-3 (Gal-3) at baseline (i.e., before treatment). The aims of the present study were to investigate the histological and molecular effects of long-term treatment (36 months) with anti-IgE and to analyze the behavior of OR and NOR patients. All patients were treated with the monoclonal ...
Azithromycin Attenuates Pseudomonas-Induced Lung Inflammation by Targeting Bacterial Proteins Secreted in the Cultured Medium
Frontiers in immunology, 2016
Pseudomonas aeruginosa airway infections are a major cause of morbidity and mortality in patients... more Pseudomonas aeruginosa airway infections are a major cause of morbidity and mortality in patients with cystic fibrosis (CF). Azithromycin improves the related clinical outcomes, but its mechanisms of action remain poorly understood. We tested the hypothesis that azithromycin downregulates P. aeruginosa-induced pro-inflammatory responses by modifying release of bacterial proteins. We monitored inflammatory markers in lungs of CF mutant mice and their littermate controls in response to conditioned media (CM) collected from the reference P. aeruginosa PAO1 strain cultured in the presence or in the absence of azithromycin. A mass spectrometry-based proteomic approach was applied to examine whether the macrolide elicits a differential release of bacterial proteins. CM collected from azithromycin-untreated PAO1 cultures induced powerful pro-inflammatory neutrophil-dominated responses. Azithromycin attenuated the responses, mainly of macrophage chemoattractant protein-1, tumor necrosis fac...
Inffluence of Structure on the Behavior of Flavonoids in Capillary Electrophoresis
Electrophoresis, 1994
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Papers by Pierluigi Mauri