Books by Philippe Laurent
La question foncière a fait, à la faveur de la hausse historique des prix, un retour en force su... more La question foncière a fait, à la faveur de la hausse historique des prix, un retour en force sur la scène de l'aménagement en Ile-de-france. Planification, grands projets, coopérations métropolitaines, établissements publics foncier, les initiatives en faveur d'une "meilleure maîtrise" du foncier régional ne manquent pas.... Entre difficultés réelles et fantasmées, cet ouvrage propose de faire le point sur dix idées reçues, dix questions pour dix auteurs, chercheurs ou praticiens, pour une approche à la fois académique et pratique d'une question au coeur des problèmatiques d'aménagement aujourd'hui et de demain
Papers by Philippe Laurent
Natural colorants are widely used in the world in many industries such as food processing.
European Journal of Biochemistry, Jul 31, 1995
In this study, we report the effect of fatty acids on the Thy-1 antigen mRNA decay. Low serum and... more In this study, we report the effect of fatty acids on the Thy-1 antigen mRNA decay. Low serum and synthetic medium culture conditions were used to demonstrate that fatty acids, which are important metabolites involved as second messengers in signal transduction, also influence the steady-state mRNA level. Detailed analysis demonstrated that polyunsaturated lipids attached to bovine serum albumin, such as linoleic, linolenic, and arachidonic acids, modulate gene expression specifically in the S1A T lymphoma cell line by inducing a 3-5-fold increase in the steady-state Thy-1 mRNA level, concomitant with a twofold increase in cell surface expression. A similar modulation was observed in the immature CD4-CD8-T cell precursors but not in mature thymocytes. Nuclear run-on and transfection experiments indicated that the observed Thy-1 mRNA level is post-transcriptionally regulated and that the presence of the coding region is sufficient for this adaptive response. A mechanism without a requirement for protein kinase C activation, but involving Ca2+ entry, could account for this difference in Thy-1 mRNA stability.
ABSTRACT Pigments produced by the bacteria belonging to the genus Arthrobacter. Sutthiwong N., Ca... more ABSTRACT Pigments produced by the bacteria belonging to the genus Arthrobacter. Sutthiwong N., Caro Y., Fouillaud M., Laurent P., Valla A., Dufossé L.. Proceedings of the 7th International Congress of Pigments in Food – New technologies towards health, through colors, Novara, Italy, June 18-21 2013. Coïsson J.D., Travaglia F. Arlorio M. (Eds.), Università degli Studi del Piemonte Orientale “A. Avogadro”, Novara, Italy (Publ.), ISBN 978-88-903360-3-4, 122-126, 2013.
Two colorimetric methods were compared for assaying the cell toxicity of soluble crystal proteins... more Two colorimetric methods were compared for assaying the cell toxicity of soluble crystal proteins from ®ve mosquitocidal serovarieties of Bacillus thuringiensis: israelensis, jegathesan, medellin, darmstadiensis and fukuokaensis. In mosquitoes cell lines from Culex quinquefasciatus, Aedes aegypti and Anopheles gambiae, all the crystal proteins were equally toxic. Both colorimetric methods MTT (tetrazolium salt) and Alamar Blue gave similar results, but the Alamar Blue method was simpler, faster, cheaper, and could be used to take readings from the same cell population at various time points. The most active crystal proteins were those of B. thuringiensis israelensis, followed by those of B. thuringiensis jegathesan and B. thuringiensis medellin, which were much less active.
Natural colorants are widely used in the world in many industries such as food processing.
ABSTRACT Pigments produced by the bacteria belonging to the genus Arthrobacter. Sutthiwong N., Ca... more ABSTRACT Pigments produced by the bacteria belonging to the genus Arthrobacter. Sutthiwong N., Caro Y., Fouillaud M., Laurent P., Valla A., Dufossé L.. Proceedings of the 7th International Congress of Pigments in Food – New technologies towards health, through colors, Novara, Italy, June 18-21 2013. Coïsson J.D., Travaglia F. Arlorio M. (Eds.), Università degli Studi del Piemonte Orientale “A. Avogadro”, Novara, Italy (Publ.), ISBN 978-88-903360-3-4, 122-126, 2013.
Space Telescopes and Instrumentation 2012: Ultraviolet to Gamma Ray, 2012
The Hard X-ray Imager (HXI) is one of four detectors on board the ASTRO-H mission (6th Japanese X... more The Hard X-ray Imager (HXI) is one of four detectors on board the ASTRO-H mission (6th Japanese X-ray satellite), which is scheduled to be launched in 2014. Using the hybrid structure composed of double-sided silicon strip detectors and a cadmium telluride double-sided strip detector, the instrument fully covers the energy range of photons collected with the hard X-ray telescope up to 80 keV with a high quantum efficiency. High spatial resolution of 250 µm and an energy resolution of 1-2 keV (FWHM) are both achieved with low noise front-end ASICs. In addition, the thick BGO active shields surrounding the main detector package is a heritage of the successful performance of the Hard X-ray Detector on board the Suzaku satellite. This feature enables the instrument to achieve an extremely high background reduction caused by cosmic-ray particles, cosmic X-ray background, and in-orbit radiation activation. In this paper, we present the detector concept, design, latest results of the detector development, and the current status of the hardware.
Space Telescopes and Instrumentation 2010: Ultraviolet to Gamma Ray, 2010
ASTRO-H is the next generation JAXA X-ray satellite, intended to carry instruments with broad ene... more ASTRO-H is the next generation JAXA X-ray satellite, intended to carry instruments with broad energy coverage and exquisite energy resolution. The Soft Gamma-ray Detector (SGD) is one of ASTRO-H instruments and will feature wide energy band (40-600 keV) at a background level 10 times better than the current instruments on orbit. SGD is complimentary to ASTRO-H's Hard X-ray Imager covering the energy range of 5-80 keV. The SGD achieves low background by combining a Compton camera scheme with a narrow field-of-view active shield where Compton kinematics is utilized to reject backgrounds. The Compton camera in the SGD is realized as a hybrid semiconductor detector system which consists of silicon and CdTe (cadmium telluride) sensors. Good energy resolution is afforded by semiconductor sensors, and it results in good background rejection capability due to better constraints on Compton kinematics. Utilization of Compton kinematics also makes the SGD sensitive to the gamma-ray polarization, opening up a new window to study properties of gamma-ray emission processes. The ASTRO-H mission is approved by ISAS/JAXA to proceed to a detailed design phase with an expected launch in 2014. In this paper, we present science drivers and concept of the SGD instrument followed by detailed description of the instrument and expected performance.
Space Telescopes and Instrumentation 2010: Ultraviolet to Gamma Ray, 2010
The Hard X-ray Imager (HXI) is one of the instruments onboard International X-ray Observatory (IX... more The Hard X-ray Imager (HXI) is one of the instruments onboard International X-ray Observatory (IXO), to be launched into orbit in 2020s. It covers the energy band of 10-40 keV, providing imaging-spectroscopy with a field of view of 8 × 8 arcmin 2 . The HXI is attached beneath the Wide Field Imager (WFI) covering 0.1-15 keV. Combined with the super-mirror coating on the mirror assembly, this configuration provides observation of X-ray source in wide energy band (0.1-40.0 keV) simultaneously, which is especially important for varying sources. The HXI sensor part consists of the semiconductor imaging spectrometer, using Si in the medium energy detector and CdTe in the high energy detector as its material, and an active shield covering its back to reduce background in orbit. The HXI technology is based on those of the Japanese-lead new generation X-ray observatory ASTRO-H, and partly from those developed for Simbol-X. Therefore, the technological development is in good progress. In the IXO mission, HXI will provide a major assets to identify the nature of the object by penetrating into thick absorbing materials and determined the inherent spectral shape in the energy band well above the structure around Fe-K lines and edges.
European Journal of Biochemistry, 1995
In this study, we report the effect of fatty acids on the Thy-1 antigen mRNA decay. Low serum and... more In this study, we report the effect of fatty acids on the Thy-1 antigen mRNA decay. Low serum and synthetic medium culture conditions were used to demonstrate that fatty acids, which are important metabolites involved as second messengers in signal transduction, also influence the steady-state mRNA level. Detailed analysis demonstrated that polyunsaturated lipids attached to bovine serum albumin, such as linoleic, linolenic, and arachidonic acids, modulate gene expression specifically in the S1A T lymphoma cell line by inducing a 3-5-fold increase in the steady-state Thy-1 mRNA level, concomitant with a twofold increase in cell surface expression. A similar modulation was observed in the immature CD4-CD8-T cell precursors but not in mature thymocytes. Nuclear run-on and transfection experiments indicated that the observed Thy-1 mRNA level is post-transcriptionally regulated and that the presence of the coding region is sufficient for this adaptive response. A mechanism without a requirement for protein kinase C activation, but involving Ca2+ entry, could account for this difference in Thy-1 mRNA stability.
Space Telescopes and Instrumentation 2012: Ultraviolet to Gamma Ray, 2012
The main objective of the Wide Field Monitor (WFM) on the LOFT mission is to provide unambiguous ... more The main objective of the Wide Field Monitor (WFM) on the LOFT mission is to provide unambiguous detection of the high-energy sources in a large field of view, in order to support science operations of the LOFT primary instrument, the LAD. The monitor will also provide by itself a large number of results on the timing and spectral behavior of hundreds of galactic compact objects, Active Galactic Nuclei and Gamma-Ray Bursts. The WFM is based on the coded aperture concept where a position sensitive detector records the shadow of a mask projected by the celestial sources. The proposed WFM detector plane, based on Double Sided micro-Strip Silicon Detectors (DSSD), will allow proper 2dimensional recording of the projected shadows. Indeed the positioning of the photon interaction in the detector with equivalent fine resolution in both directions insures the best imaging capability compatible with the allocated budgets for this telescope on LOFT. We will describe here the overall configuration of this 2D-WFM and the design and characteristics of the DSSD detector plane including its imaging and spectral performances. We will also present a number of simulated results discussing the advantages that this configuration offers to LOFT. A DSSD-based WFM will in particular reduce significantly the source confusion experienced by the WFM in crowded regions of the sky like the Galactic Center and will in general increase the observatory science capability of the mission.
Vaccine, 2007
The advantages of intradermal (ID) vaccine administration have been well documented but difficult... more The advantages of intradermal (ID) vaccine administration have been well documented but difficulties in performing ID vaccination using existing techniques and equipment have limited it&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s clinical application. In the present study, a new ID injection technique and associated microinjection system is described and evaluated in a swine and Human models. Clinical investigation models included: injection site imaging (X-ray and 3D ultrasound echography), histological examination of injection sites, fluid injection volume accuracy measurement, subject&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; perceived pain and local skin reactivity were specifically developed. These evaluations showed that microinjection system can make the practice of ID vaccination easy to perform, reliable and safe, thus setting the stage for broader clinical application of ID vaccine delivery.
The Astrophysical Journal, 1992
The Astrophysical Journal, 1993
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Books by Philippe Laurent
Papers by Philippe Laurent