Abstract Selection of winter self-pollinated plants with short to medium stem height was initiate... more Abstract Selection of winter self-pollinated plants with short to medium stem height was initiated in 15 cultivated rye populations. About 8.8% seed set per selfed spike was registered in the first two years. In inbred S 2 families, self-fertility varied between 0–73 seeds per isolated ear and plant height ranged from 76 to 115 cm. Response to selection and genetic advance in percent of the mean characterized the S 3 families for one cycle of selection. SDS-PAGE gel patterns showed two major protein bands for the rye HMW secalins—one slowly moving x-subunit expressed as 2r, 5.2*r and 5.3r, and the second quickly moving y-subunit, expressed as 6r, 6.5r, 7r and 9r. Regarding alleles in Glu-R1 and Gli-R2 loci, nine progenies appeared to show genetic homogeneity in proteins, supporting by low coefficients of variation for plant height. The HMW compositions 2r (alone band) and 5.3r + 7r, were defined as new secalin subunits. 75 K γ-secalins, encoded at Gli-R2 , were composed by alleles a , b , c , for subunits d1, d2 and t1, respectively. The results showed that among all, four selfed rye progenies were considered as homogeneous genotypes and could be used as inbred lines in further genetic and breeding experiments.
High-molecular-weight glutenin subunits (HMW-GS) encoded by alleles at the Glu-A1, Glu-B1, and Gl... more High-molecular-weight glutenin subunits (HMW-GS) encoded by alleles at the Glu-A1, Glu-B1, and Glu-D1 loci confer unique bread-making properties of common wheat (Triticum aestivum L.). The identification of HMW-GS is the prerequisite for pyramiding high-quality glutenin genes. The present review is designed to list all published HMW-GS alleles in Triticum species and triticale (A-and B genomes), focusing on methods for their identification. T. monococcum is characterized by 37 alleles at the Glu-1 locus versus four alleles in T. thaoudar and 39 in T. urartu. In total, 80 alleles at Glu-A1 of diploid Triticum species and about 42 alleles found in polyploid wheat landraces and varieties (4x and 6x), including triticale, were listed. Allelic variation at the Glu-B1 locus is divided into 3 groups: a-z, aa-az, and ba-ct, comprising
This study characterized six introgression lines obtained from crossing two winter bread wheat va... more This study characterized six introgression lines obtained from crossing two winter bread wheat varieties Albena and Slaveya with the D-genome synthetic hexaploid wheat 530-1. Phenotypically, the lines differed from the parents,as five of them expressed red spike color versus line 45-5 producing new coloration, identified as striped or bandedglume pigmentation. All lines were taller, with longer ears and spikelets, later in heading time and less productive in grain yield than both wheat parents. They showed some positive traits in the field: optimal test weight (78–80 kg/hl), high thousand kernel weight (48–51 g), low sprouted seeds (0.3–1.5%), and high falling number (314-350 s). Two lines (38-7 and 46-7) exceeded the parents in grain protein content. In the competitive variety trial, Albena showed significantly
higher sprouted grains (4.1%) and extreme measurements for a percentage of germination (99.0%) and germination index (83.0%) in 2022. The data identified Albena as a wheat variety with pre-harvest sprouting susceptibility. Of the lines, only 45-5 stood out with the lowest germination values and appeared as a promising genotype with resistance to pre-harvest sprouting. The introgression lines exerted useful genetic diversity for the studied traits and could serve as resources for quality improvement in wheat breeding programs.
A Triticum durum – T. monococcum amphiploid (AABBAmAm) was synthesized by chromosome doubling of ... more A Triticum durum – T. monococcum amphiploid (AABBAmAm) was synthesized by chromosome doubling of sterile triploid hybrids obtained between the two T. durum cultivars Saturn-1 and Neptun-2, and one T. monococcum ssp. aegilopoides (AmAm) accession. The two amphiploid lines, A1 and A2 with each durum cultivar, expressed resistance to wheat powdery mildew at seedling and adult stage, and to four leaf rust cultures, including isolate 22762 to which the diploid wild wheat was susceptible. The newly produced amphiploid could be a useful genetic resource for fungal resistance in wheat.
In order to identifY the alien chromosome introgressed into the wheat's genome of the aneuploid l... more In order to identifY the alien chromosome introgressed into the wheat's genome of the aneuploid lines of the cross: Triticum aestivum cv Kilen ?4ABBDD) x Aegilops kotschyi (UUS~~') a protein polymorphism (gliadins and glutenins) as markers for 1-st and 6-th homoeologous group is used. An SDS-PAGE and a native 1-D-slab PAGE in the case ofglutenins and gliagins respectively are performed. Some additional bands specific for Ae.kotschyi as well as a comparison to Ae. umbellulata (UU), Ae. longissima (s1S 1), Ae. squrrosa (DD) and Chinese Spring nullitetrasomic lines indicate the introgression of U genome in the investigated disomic addition and substitution lines. The results of this study show that the polymorphism of the storage proteins can be used as a reliable marker system for quick and cheaper screening of the presence of alien chromosomes.
Rye (Secale L.) is a member of family Poaceae (tribe Triticeae) and includes perennial or annual,... more Rye (Secale L.) is a member of family Poaceae (tribe Triticeae) and includes perennial or annual, self-incompatible or self-compatible, and cultivated, weedy or wild species. Classification of the genus Secale is inconsistent, and comprises 3-4 to 8 species from the phylogenetic studies in the last ten years. Progress in rye breeding has been significantly reduced due to involving a small number of cultivars and landraces in crosses. The wild rye species and subspecies possess many valuable breeding traits for research aimed at expanding the variability in Secale cereale subsp. cereale. They are, due to their genetic diversity and high breeding trait expression, useful sources of genes for tetraploid and hexaploid wheat, and triticale improvement, too. One of the species, S. vavilovii, is attractive for rye breeding due to its high self-fertility, resistance to fusarium ear blight, septoria leaf blotch, high protein content, sprouting and sterilising cytoplasm, and genetic similarity with S. cereale subsp. dighoricum. Chromosomes of S. strictum are sources for resistance to yellow rust, Russian wheat aphid, grain hardness, increased protein and arabinoxylan content.
Lines with resistance to powdery mildew in adult plant stage have been selected from two groups o... more Lines with resistance to powdery mildew in adult plant stage have been selected from two groups of F1 seeds produced by crossing T. aestivum with Aegilops variabilis (2n=28, UUSS). Two lines were derived from nonirradiated F1 seeds and another five lines originated from irradiated hybrid seeds. Rusalka and Pliska bread wheat varieties were used as backcrossing parents. Two lines selected from the irradiated group, showed different resistance pattern in comparison to 18 wheat cultivars/lines possessing known powdery mildew resistance genes. Line 11-8 was previously identified with C-banding as a 1U(1B) substitution. It expressed a distinctive disease pattern, different from the parents and differential set of lines. It is suggested that the alien 1U chromosome is a carrier of resistance to powdery mildew, as evidenced by the disease pattern of doubled haploid lines derived from cv. Charodeika x line 11-8 cross. The DH lines expressed resistance to 8 from 11 isolates used. Additionall...
Free-threshing einkorn wheat (Triticum sinskajae A. Filat. et Kurk.) is an excellent source of re... more Free-threshing einkorn wheat (Triticum sinskajae A. Filat. et Kurk.) is an excellent source of resistance against wheat diseases and grain quality parameters. A new synthetic hexaploid amphiploid SHW45 (T. durum 198-2 × Triticum sinskajae acc. 18397) was studied in C 2-7 generations and characterised for field growing. Some traits such as a plant height and spike features including fertility and spike fragility revealed a large phenotypic variation. A strong selection for resistance to spikelet dispersal and a number of seeds per selfed spike led to the creation of several lines. They manifested a variety of resistance to powdery mildew at the seedling stage. Three amphiploid lines with diverse phenotypes and a complete resistance to spike brittleness were crossed as females to the wheat cultivar 'Sadovska ranozreika-4'. Viable hybrid seeds are obtained only when the synthetic form is a female parent. F 1 hybrids of amphiploid line 45-10 bore an average of 9 seeds per isolated spike, which was a significantly better yield than that of other hybrids. The lines showed homogeneity in high molecular weight (HMW) glutenins, as subunit pairs Ax2*+Ay1 and Bx17+By18 are inherited from the T. sinskajae, and T. durum form 198-2, respectively. In total, 14 F 2 , 22 BC 1 , and 141 F 2-BC 1 seeds from the crosses with two bread wheat cultivars were planted in the field. The synthetic amphiploid lines might be promising genetic resources for resistance to powdery mildew and grain quality in wheat breeding.
Journal of Agricultural, Food and Environmental Sciences, 2018
The effect of the new fraction pair 1.5+10, coded by Glu-D1 locus, on the qualitative indices pro... more The effect of the new fraction pair 1.5+10, coded by Glu-D1 locus, on the qualitative indices protein, lysine and sedimentation value in synthetic hexaploid wheat varieties was investigated in comparison to common winter wheat standards. For this purpose, in two successive cropping seasons (2013 and 2014), grains from plants of the progenies of the synthetic haploid wheat genotypes 106, 107 and 32 were analyzed; preliminary SDS-PAGE analysis determined that they were carriers of the new allele ah inherited from the Glu-D t 1 locus of Ae. tauschii . Data were analyzed with the help of descriptive statistics, t-criterion, correlation and regression analyses, which were at the basis of the conclusions made. The obtained results allow the assumption that in the synthetic hexaploid wheat genotypes the new allele ah , coding for the expression of the new fraction pair 1.5+10 in D-genome, has positive effect on the qualitative indices protein, lysine and sedimentation in contrast to the st...
Pathogens are major constraints for wheat and triticale production. This review provides a presen... more Pathogens are major constraints for wheat and triticale production. This review provides a present state of knowledge about pathogen resistance genes in wheat-rye and triticale genetic stocks, revealing their initial Secale parent, type of the inserted alien chromosome, designated specific molecular marker, if available, and resulted wheat and/or triticale lines for deployment the resistance in breeding. At present, 30 identified and temporarily designated resistance genes to diseases are listed: 13 in 1RS, 9 in 2R, 5 in 3R and 3 in 6R chromosomes. About 58 resistance loci have been arranged to single rye chromosomes, belonging to different accessions of S. cereale , africanum , cereanum , montanum and strictum , and certain triticale varieties. It is obvious that production of more 1BL.1RS translocations from different rye varieties will enhance the genetic diversity of wheat with respect to resistance against fungal and viral diseases. The recorded addition, substitution and translocation lines, some of them with specific chromosome arm markers, can efficiently serve as important bridges for wheat and triticale improvement. The information thus provided will help plant pathologists to develop resistant lines with rye chromatin through classical and marker-assisted breeding.
... 1988; MULTANI et al. 1988; SPETSOV and SAVOV 1992). ... 2006; STOILOVA and SPETSOV 2006). The... more ... 1988; MULTANI et al. 1988; SPETSOV and SAVOV 1992). ... 2006; STOILOVA and SPETSOV 2006). The common wheat variety Sadovska ranozreika-4 as pathogen spreader and stan-dard for high susceptibility to powdery mildew, was placed at several sites in the greenhouse. ...
Winter hexaploid wheat (Triticum aestivum L.) was crossed with Aegilops variabilis to transfer re... more Winter hexaploid wheat (Triticum aestivum L.) was crossed with Aegilops variabilis to transfer resistance to powdery mildew into wheat. Following two backcrosses to wheat and from 5 to 9 generations of selfing, several disomic addition and substitution lines of hexaploid wheat resistant to the mildew pathogen were isolated. A pair of short satellited chromosomes was always observed in the resistant lines. Further evidence utilizing as markers for homoeologous group 1 HMW glutenin subunits and DNA hybridization with probe pGBX 3076 showed that an alien substitution involved this homoeologous group.
DOI to the publisher's website. • The final author version and the galley proof are versions of t... more DOI to the publisher's website. • The final author version and the galley proof are versions of the publication after peer review. • The final published version features the final layout of the paper including the volume, issue and page numbers. Link to publication General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal. If the publication is distributed under the terms of Article 25fa of the Dutch Copyright Act, indicated by the "Taverne" license above, please follow below link for the End User Agreement:
The production and isolation of a wheat-Aegilops geniculata disomic addition line and of monosomi... more The production and isolation of a wheat-Aegilops geniculata disomic addition line and of monosomic substitution line resistant to powdery mildew at the seedling and adult plant stages are described. Cytological analysis revealed the presence of an acrocentric chromosome in the resistant derivatives. Polyacrylamide gel electrophoretic analysis indicated differences in the β-zone of the reduced by mercaptoethanol treatment seed protein pattern between the wheat parent cv. Trakya and the resistant derivatives. A component with a high staining intensity in the β-zone was assumed to be a marker for the chromosome 6 of Ae. geniculata. The isoenzyme activity at the Got-1 and Est-4 loci of the lines was lower than that of wheat. By analyzing the isoenzymes of the Amp-1 locus of the addition plants, a quantitative marker of Ae. geniculata 6U chromosome was identified. The progeny of the plants with the 2n = 43 and 2n = 42 chromosomes carrying the chromosome 6U added or mono-substituted for the wheat 6D chromosome segregated for powdery mildew resistance. The mono-and/or double conditions for this chromosome could be maintained by selecting resistant plants in the segregated populations.
Abstract Selection of winter self-pollinated plants with short to medium stem height was initiate... more Abstract Selection of winter self-pollinated plants with short to medium stem height was initiated in 15 cultivated rye populations. About 8.8% seed set per selfed spike was registered in the first two years. In inbred S 2 families, self-fertility varied between 0–73 seeds per isolated ear and plant height ranged from 76 to 115 cm. Response to selection and genetic advance in percent of the mean characterized the S 3 families for one cycle of selection. SDS-PAGE gel patterns showed two major protein bands for the rye HMW secalins—one slowly moving x-subunit expressed as 2r, 5.2*r and 5.3r, and the second quickly moving y-subunit, expressed as 6r, 6.5r, 7r and 9r. Regarding alleles in Glu-R1 and Gli-R2 loci, nine progenies appeared to show genetic homogeneity in proteins, supporting by low coefficients of variation for plant height. The HMW compositions 2r (alone band) and 5.3r + 7r, were defined as new secalin subunits. 75 K γ-secalins, encoded at Gli-R2 , were composed by alleles a , b , c , for subunits d1, d2 and t1, respectively. The results showed that among all, four selfed rye progenies were considered as homogeneous genotypes and could be used as inbred lines in further genetic and breeding experiments.
High-molecular-weight glutenin subunits (HMW-GS) encoded by alleles at the Glu-A1, Glu-B1, and Gl... more High-molecular-weight glutenin subunits (HMW-GS) encoded by alleles at the Glu-A1, Glu-B1, and Glu-D1 loci confer unique bread-making properties of common wheat (Triticum aestivum L.). The identification of HMW-GS is the prerequisite for pyramiding high-quality glutenin genes. The present review is designed to list all published HMW-GS alleles in Triticum species and triticale (A-and B genomes), focusing on methods for their identification. T. monococcum is characterized by 37 alleles at the Glu-1 locus versus four alleles in T. thaoudar and 39 in T. urartu. In total, 80 alleles at Glu-A1 of diploid Triticum species and about 42 alleles found in polyploid wheat landraces and varieties (4x and 6x), including triticale, were listed. Allelic variation at the Glu-B1 locus is divided into 3 groups: a-z, aa-az, and ba-ct, comprising
This study characterized six introgression lines obtained from crossing two winter bread wheat va... more This study characterized six introgression lines obtained from crossing two winter bread wheat varieties Albena and Slaveya with the D-genome synthetic hexaploid wheat 530-1. Phenotypically, the lines differed from the parents,as five of them expressed red spike color versus line 45-5 producing new coloration, identified as striped or bandedglume pigmentation. All lines were taller, with longer ears and spikelets, later in heading time and less productive in grain yield than both wheat parents. They showed some positive traits in the field: optimal test weight (78–80 kg/hl), high thousand kernel weight (48–51 g), low sprouted seeds (0.3–1.5%), and high falling number (314-350 s). Two lines (38-7 and 46-7) exceeded the parents in grain protein content. In the competitive variety trial, Albena showed significantly
higher sprouted grains (4.1%) and extreme measurements for a percentage of germination (99.0%) and germination index (83.0%) in 2022. The data identified Albena as a wheat variety with pre-harvest sprouting susceptibility. Of the lines, only 45-5 stood out with the lowest germination values and appeared as a promising genotype with resistance to pre-harvest sprouting. The introgression lines exerted useful genetic diversity for the studied traits and could serve as resources for quality improvement in wheat breeding programs.
A Triticum durum – T. monococcum amphiploid (AABBAmAm) was synthesized by chromosome doubling of ... more A Triticum durum – T. monococcum amphiploid (AABBAmAm) was synthesized by chromosome doubling of sterile triploid hybrids obtained between the two T. durum cultivars Saturn-1 and Neptun-2, and one T. monococcum ssp. aegilopoides (AmAm) accession. The two amphiploid lines, A1 and A2 with each durum cultivar, expressed resistance to wheat powdery mildew at seedling and adult stage, and to four leaf rust cultures, including isolate 22762 to which the diploid wild wheat was susceptible. The newly produced amphiploid could be a useful genetic resource for fungal resistance in wheat.
In order to identifY the alien chromosome introgressed into the wheat's genome of the aneuploid l... more In order to identifY the alien chromosome introgressed into the wheat's genome of the aneuploid lines of the cross: Triticum aestivum cv Kilen ?4ABBDD) x Aegilops kotschyi (UUS~~') a protein polymorphism (gliadins and glutenins) as markers for 1-st and 6-th homoeologous group is used. An SDS-PAGE and a native 1-D-slab PAGE in the case ofglutenins and gliagins respectively are performed. Some additional bands specific for Ae.kotschyi as well as a comparison to Ae. umbellulata (UU), Ae. longissima (s1S 1), Ae. squrrosa (DD) and Chinese Spring nullitetrasomic lines indicate the introgression of U genome in the investigated disomic addition and substitution lines. The results of this study show that the polymorphism of the storage proteins can be used as a reliable marker system for quick and cheaper screening of the presence of alien chromosomes.
Rye (Secale L.) is a member of family Poaceae (tribe Triticeae) and includes perennial or annual,... more Rye (Secale L.) is a member of family Poaceae (tribe Triticeae) and includes perennial or annual, self-incompatible or self-compatible, and cultivated, weedy or wild species. Classification of the genus Secale is inconsistent, and comprises 3-4 to 8 species from the phylogenetic studies in the last ten years. Progress in rye breeding has been significantly reduced due to involving a small number of cultivars and landraces in crosses. The wild rye species and subspecies possess many valuable breeding traits for research aimed at expanding the variability in Secale cereale subsp. cereale. They are, due to their genetic diversity and high breeding trait expression, useful sources of genes for tetraploid and hexaploid wheat, and triticale improvement, too. One of the species, S. vavilovii, is attractive for rye breeding due to its high self-fertility, resistance to fusarium ear blight, septoria leaf blotch, high protein content, sprouting and sterilising cytoplasm, and genetic similarity with S. cereale subsp. dighoricum. Chromosomes of S. strictum are sources for resistance to yellow rust, Russian wheat aphid, grain hardness, increased protein and arabinoxylan content.
Lines with resistance to powdery mildew in adult plant stage have been selected from two groups o... more Lines with resistance to powdery mildew in adult plant stage have been selected from two groups of F1 seeds produced by crossing T. aestivum with Aegilops variabilis (2n=28, UUSS). Two lines were derived from nonirradiated F1 seeds and another five lines originated from irradiated hybrid seeds. Rusalka and Pliska bread wheat varieties were used as backcrossing parents. Two lines selected from the irradiated group, showed different resistance pattern in comparison to 18 wheat cultivars/lines possessing known powdery mildew resistance genes. Line 11-8 was previously identified with C-banding as a 1U(1B) substitution. It expressed a distinctive disease pattern, different from the parents and differential set of lines. It is suggested that the alien 1U chromosome is a carrier of resistance to powdery mildew, as evidenced by the disease pattern of doubled haploid lines derived from cv. Charodeika x line 11-8 cross. The DH lines expressed resistance to 8 from 11 isolates used. Additionall...
Free-threshing einkorn wheat (Triticum sinskajae A. Filat. et Kurk.) is an excellent source of re... more Free-threshing einkorn wheat (Triticum sinskajae A. Filat. et Kurk.) is an excellent source of resistance against wheat diseases and grain quality parameters. A new synthetic hexaploid amphiploid SHW45 (T. durum 198-2 × Triticum sinskajae acc. 18397) was studied in C 2-7 generations and characterised for field growing. Some traits such as a plant height and spike features including fertility and spike fragility revealed a large phenotypic variation. A strong selection for resistance to spikelet dispersal and a number of seeds per selfed spike led to the creation of several lines. They manifested a variety of resistance to powdery mildew at the seedling stage. Three amphiploid lines with diverse phenotypes and a complete resistance to spike brittleness were crossed as females to the wheat cultivar 'Sadovska ranozreika-4'. Viable hybrid seeds are obtained only when the synthetic form is a female parent. F 1 hybrids of amphiploid line 45-10 bore an average of 9 seeds per isolated spike, which was a significantly better yield than that of other hybrids. The lines showed homogeneity in high molecular weight (HMW) glutenins, as subunit pairs Ax2*+Ay1 and Bx17+By18 are inherited from the T. sinskajae, and T. durum form 198-2, respectively. In total, 14 F 2 , 22 BC 1 , and 141 F 2-BC 1 seeds from the crosses with two bread wheat cultivars were planted in the field. The synthetic amphiploid lines might be promising genetic resources for resistance to powdery mildew and grain quality in wheat breeding.
Journal of Agricultural, Food and Environmental Sciences, 2018
The effect of the new fraction pair 1.5+10, coded by Glu-D1 locus, on the qualitative indices pro... more The effect of the new fraction pair 1.5+10, coded by Glu-D1 locus, on the qualitative indices protein, lysine and sedimentation value in synthetic hexaploid wheat varieties was investigated in comparison to common winter wheat standards. For this purpose, in two successive cropping seasons (2013 and 2014), grains from plants of the progenies of the synthetic haploid wheat genotypes 106, 107 and 32 were analyzed; preliminary SDS-PAGE analysis determined that they were carriers of the new allele ah inherited from the Glu-D t 1 locus of Ae. tauschii . Data were analyzed with the help of descriptive statistics, t-criterion, correlation and regression analyses, which were at the basis of the conclusions made. The obtained results allow the assumption that in the synthetic hexaploid wheat genotypes the new allele ah , coding for the expression of the new fraction pair 1.5+10 in D-genome, has positive effect on the qualitative indices protein, lysine and sedimentation in contrast to the st...
Pathogens are major constraints for wheat and triticale production. This review provides a presen... more Pathogens are major constraints for wheat and triticale production. This review provides a present state of knowledge about pathogen resistance genes in wheat-rye and triticale genetic stocks, revealing their initial Secale parent, type of the inserted alien chromosome, designated specific molecular marker, if available, and resulted wheat and/or triticale lines for deployment the resistance in breeding. At present, 30 identified and temporarily designated resistance genes to diseases are listed: 13 in 1RS, 9 in 2R, 5 in 3R and 3 in 6R chromosomes. About 58 resistance loci have been arranged to single rye chromosomes, belonging to different accessions of S. cereale , africanum , cereanum , montanum and strictum , and certain triticale varieties. It is obvious that production of more 1BL.1RS translocations from different rye varieties will enhance the genetic diversity of wheat with respect to resistance against fungal and viral diseases. The recorded addition, substitution and translocation lines, some of them with specific chromosome arm markers, can efficiently serve as important bridges for wheat and triticale improvement. The information thus provided will help plant pathologists to develop resistant lines with rye chromatin through classical and marker-assisted breeding.
... 1988; MULTANI et al. 1988; SPETSOV and SAVOV 1992). ... 2006; STOILOVA and SPETSOV 2006). The... more ... 1988; MULTANI et al. 1988; SPETSOV and SAVOV 1992). ... 2006; STOILOVA and SPETSOV 2006). The common wheat variety Sadovska ranozreika-4 as pathogen spreader and stan-dard for high susceptibility to powdery mildew, was placed at several sites in the greenhouse. ...
Winter hexaploid wheat (Triticum aestivum L.) was crossed with Aegilops variabilis to transfer re... more Winter hexaploid wheat (Triticum aestivum L.) was crossed with Aegilops variabilis to transfer resistance to powdery mildew into wheat. Following two backcrosses to wheat and from 5 to 9 generations of selfing, several disomic addition and substitution lines of hexaploid wheat resistant to the mildew pathogen were isolated. A pair of short satellited chromosomes was always observed in the resistant lines. Further evidence utilizing as markers for homoeologous group 1 HMW glutenin subunits and DNA hybridization with probe pGBX 3076 showed that an alien substitution involved this homoeologous group.
DOI to the publisher's website. • The final author version and the galley proof are versions of t... more DOI to the publisher's website. • The final author version and the galley proof are versions of the publication after peer review. • The final published version features the final layout of the paper including the volume, issue and page numbers. Link to publication General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal. If the publication is distributed under the terms of Article 25fa of the Dutch Copyright Act, indicated by the "Taverne" license above, please follow below link for the End User Agreement:
The production and isolation of a wheat-Aegilops geniculata disomic addition line and of monosomi... more The production and isolation of a wheat-Aegilops geniculata disomic addition line and of monosomic substitution line resistant to powdery mildew at the seedling and adult plant stages are described. Cytological analysis revealed the presence of an acrocentric chromosome in the resistant derivatives. Polyacrylamide gel electrophoretic analysis indicated differences in the β-zone of the reduced by mercaptoethanol treatment seed protein pattern between the wheat parent cv. Trakya and the resistant derivatives. A component with a high staining intensity in the β-zone was assumed to be a marker for the chromosome 6 of Ae. geniculata. The isoenzyme activity at the Got-1 and Est-4 loci of the lines was lower than that of wheat. By analyzing the isoenzymes of the Amp-1 locus of the addition plants, a quantitative marker of Ae. geniculata 6U chromosome was identified. The progeny of the plants with the 2n = 43 and 2n = 42 chromosomes carrying the chromosome 6U added or mono-substituted for the wheat 6D chromosome segregated for powdery mildew resistance. The mono-and/or double conditions for this chromosome could be maintained by selecting resistant plants in the segregated populations.
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Papers by Penko Spetsov
higher sprouted grains (4.1%) and extreme measurements for a percentage of germination (99.0%) and germination index (83.0%) in 2022. The data identified Albena as a wheat variety with pre-harvest sprouting susceptibility. Of the lines, only 45-5 stood out with the lowest germination values and appeared as a promising genotype with resistance to pre-harvest sprouting. The introgression lines exerted useful genetic diversity for the studied traits and could serve as resources for quality improvement in wheat breeding programs.
higher sprouted grains (4.1%) and extreme measurements for a percentage of germination (99.0%) and germination index (83.0%) in 2022. The data identified Albena as a wheat variety with pre-harvest sprouting susceptibility. Of the lines, only 45-5 stood out with the lowest germination values and appeared as a promising genotype with resistance to pre-harvest sprouting. The introgression lines exerted useful genetic diversity for the studied traits and could serve as resources for quality improvement in wheat breeding programs.