Papers by Deepshikha Patle
Indian journal of pharmacy and pharmacology, Oct 14, 2023
Background: A stability indicating high performance liquid chromatographic method has been develo... more Background: A stability indicating high performance liquid chromatographic method has been developed and validated for the anti-diabetic drugs Rosiglitazone, Glimepiride, and Metformin HCl in pharmaceutical dosage forms. Methods: Chromatographic separation was achieved on Zorbex SB C-8 (250 X 4.6 mm) 5µ and Hypersil BDS C18 (200 × 4 mm), 5µ column as stationary phase. Mobile phase consisting of 0.023M potassium dihydrogen phosphate and acetonitrile (60:40, v/v) supplied at a flow rate of 1ml/min. Detection was performed using a SPD-20A prominence UV/VIS detector at 230 nm. Result: The retention time of rosiglitazone, glimepiride and metformin hydrochloride was achieved at 2.4 min, 4.5 min and 5.6 min respectively. The method was validated for linearity, precision, accuracy, toughness, specificity, and forced degradation studies and the relative response factor values of rosiglitazone, glimepiride, and metformin determined from linearity study were 0.998 in the combined form. Rosiglitazone, glimepiride, and metformin HCl showed percentage recoveries of 99.73%, 99.81and 100.31%, respectively. The propesd method found to be very effective and stable for the routine analysis of mentioned antidiabetic drugs in pharmaceutical dosage forms. This is an Open Access (OA) journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
Chemistry Africa, Jun 18, 2024
Journal of Molecular Structure, Apr 1, 2023
Asian Pacific journal of health sciences, Jun 30, 2022
ABSTRACT: A new simple and rapid reverse phase high-performance liquid chromatographic (RP-HPLC) ... more ABSTRACT: A new simple and rapid reverse phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous estimation of cefpodoxime proxetil in bulk drugs and pharmaceutical dosage form. The separation was accomplished on a sunfire C-18 (250x 4.6 mm, 5 μm) column with a mobile phase consisting of Methanol; Water (70:30 v/v) at a flow rate of 0.8 mL/min. The eluents were monitored at 236 nm by using PDA detector. The retention time for cefpodoxime proxetil were found to be 4.5min., respectively. The cefpodoxime proxetil followed linearity in the concentration range of 5-30 μg/mL (r2 = 0.998). The amount of drug estimated by proposed method was found to be in good agreement with label claim. The developed method was validated for sensitivity, accuracy, precision. The LOD and LOQ values were found to be 0.0004 and 0.0012 μg respectively for cefpodoxime proxetil. The proposed method can be used for routine
Current Trends in Biotechnology and Pharmacy, 2017
A simple, rapid, accurate and precise spectrophotometric method has been developed for simultaneo... more A simple, rapid, accurate and precise spectrophotometric method has been developed for simultaneous estimation of Montelukast Sodium and Fexofenadine HCl in Pure and tablet dosage form. Proposed method involves formation of ‘simultaneous equations’ at 259.60nm for Fexofenadine Hydrochloride and 283.00 nm for Montelukast Sodium using methanol as a solvent. The linearity was observed in the concentration range of 30–120 mg/ml for Fexofenadine HCl and 6–20 mg/ml for Montelukast Sodium. The correlation coefficient was found to be 0.9927 for fexofenadine HCl and 0.9985 for Montelukast Sodium. Thus the proposed method is reproducible which can be suitably applied for the estimation of FEXO &MONT in combined dosage forms. The results of analysis have been validated statistically and by recovery studies.
A simple, rapid, accurate and precise spectrophotometric method has been developed for simultaneo... more A simple, rapid, accurate and precise spectrophotometric method has been developed for simultaneous estimation of Montelukast Sodium and Fexofenadine HCl in Pure and tablet dosage form. Proposed method involves formation of ‘simultaneous equations’ at 259.60nm for Fexofenadine Hydrochloride and 283.00 nm for Montelukast Sodium using methanol as a solvent. The linearity was observed in the concentration range of 30-120 mg/ml for Fexofenadine HCl and 6-20 mg/ml for Montelukast Sodium. The correlation coefficient was found to be 0.9927 for fexofenadine HCl and 0.9985 for Montelukast Sodium. Thus the proposed method is reproducible which can be suitably applied for the estimation of FEXO &MONT in combined dosage forms. The results of analysis have been validated statistically and by recovery studies. Key-Words: Fexofenadine HCl (FEXO), Montelukast Sodium (MONT), Simultaneous equation method, spectrophotometry Introduction Fexofenadine hydrochloride (FEXO) (Figure 1)(RS)-2-[4[1-Hydroxy-4...
The aim of the study is to develop and validate a simple, precise and rapid high performance thin... more The aim of the study is to develop and validate a simple, precise and rapid high performance thin layer chromatographic (HPTLC) method for Cefpodoxime Proxetil and Ofloxacin in bulk and combined tablet dosage form. Chromatographic separation was accomplished by using precoated silica gel 60F 254 TLC plate (20cm x10cm) with 250μm thickness with mobile phase Chloroform in ratio methanol (9:1 v/v). Detection was performed at isobestic point 255 nm for both drugs. The retesion factor of Cefpodoxime Proxetil and Ofloxacin were found to be 0.63 and 0.25, respectively. The reliability of the method was assessed by evaluation of linearity range (500-3000 ng/spot for both Cefpodoxime proxetil and ofloxacin respectively. Accuracy (99.56 % for Cefpodoxime Proxetil and 99.33% for Ofloxacin), and specificity, in compliance with ICH guidelines.
The Journal of Phytopharmacology, 2020
Cancer vaccines are on the verge of success. It is a unique approach wherein patient’s immune sys... more Cancer vaccines are on the verge of success. It is a unique approach wherein patient’s immune system is dynamized to mount an immune response against the tumor mass thus, an anti-tumor effect is exhibited. Due to the down-regulation of immune system caused by the check-points such as CTLA-4 and PDL-1, cancer cells escape the immune system and began its uncontrolled growth and ultimately turns into a tumor. Even though the other treatments are available for the breast cancer chemotherapy but still the mortality and relapse incidents are sky-high. So a more personalized pharmakon is developed so as to arouse an immune response against the tumor. The main aim for developing cancer vaccines is breast cancer is to exhilarate patient’s own immune system so as to kill cancer cells with minimal side-effects. Appended leverage of using immunotherapy includes meticulous specificity, low toxicity, and the potential for permanent treatment effect due to immunologic memory. Various types of vacc...
Current Diabetes Reviews, 2021
Aim: We aimed to review the importance of the natural products and herbs used in the management o... more Aim: We aimed to review the importance of the natural products and herbs used in the management of diabetes mellitus (DM) as medicinal agents. Background: Naturally occuring phytoactive compounds and herbs are very important because they are found to be effective against several diseases. DM is a commonly occurring endocrinological disorder, with the incidences increased four times in the last 34 years. There are several oral hypoglycemic agents available in the market, which in the long term, may lead to a high risk of secondary failure rate. Objectives: This review focuses on natural products and herbs application for effective management of diabetic conditions, and natural products that can be utilized as alternative therapy. Methods: We searched the various online databases (PubMed, Bentham, ScienceDirect) and scientific publications from the library using a qualitative systematic review. The criteria of the review were based on natural products and herbs application for possess...
Frontiers in Endocrinology, 2017
Breast cancer (BC) is a persistent global challenge for its high frequency in women (although it ... more Breast cancer (BC) is a persistent global challenge for its high frequency in women (although it seldom occurs in men), due to the large diffusion of risk factors and gene mutations, and for its peculiar biology and microenvironment. To date, BC can benefit from different therapeutic strategies involving surgery, ablation, chemotherapy, radiotherapy, and more specific approaches such as hormone therapy and the administration of various substances impairing cancer growth, aggressivity, and recurrence with different modalities. Despite these relatively wide chances, also used in combinatory protocols, relevant mortality and relapse rates, often associated with resistant phenotypes, stress the need for a personalized-medicine based on prompting the patient's immune system (IS) against cancer cells. BC immunogenicity was latterly proven, so the whole immunotherapy field for BC is still at a very early stage. This immunotherapeutic approach exploits both the high specificity of adaptive immune response and the immunological memory. This review is focused on some of the majorly relevant BC vaccines available (NeuVax, AVX901, and INO-1400), providing a description of the more promising clinical trials. The efficacy of cancer vaccines highly depends on the patient's IS, and a wide optimization is needed in terms of targets' selection, drug design and combinations, dose finding, protocol structuring, and patients' recruitment; moreover, new standards are being discussed for the outcome evaluation. However, early-phases excellent results suggest that the manipulation of the IS via specific vaccines is a highly attractive approach for BC.
Current Trends in Biotechnology and Pharmacy, 2020
Glaucoma is one of the most common ophthalmic conditions encountered in primary and secondary car... more Glaucoma is one of the most common ophthalmic conditions encountered in primary and secondary care. The glaucoma is a group of progressive optic neuropathies characterized by degeneration of retinal ganglion cells and resulting changes in the optic nerve head. It is usually associated with an increase in intraocular pressure (IOP) above the normal value—usually estimated at 21 mm Hg. Loss of ganglion cells is related to the level of intraocular pressure, but other factors may also play a role. Reduction of intraocular pressure is the only proven method to treat the disease. Although treatment is usually initiated with ocular hypotensive drops, laser trabeculoplasty and surgery may also be used to slow disease progression. Introduction Glaucoma is a leading cause of irreversible blindness throughout the world. In India glaucoma constitute 2% of total blindness (1). Moreover, the management of glaucoma has an enormous impact in our society in terms of patient’s morbidity, loss of prod...
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Papers by Deepshikha Patle