Papers by Orlando Vieira de Sousa
Ciências da vida: Estudo das plantas, animais e seres humanos 2
Centaurea benedicta L., commonly known as “cardo santo,” is used as a tonic, antidepressant, anti... more Centaurea benedicta L., commonly known as “cardo santo,” is used as a tonic, antidepressant, anti-inflammatory, antibacterial, and antiseptic in traditional medicine. This study evaluated the topical anti-inflammatory potential of an extract (ECB) and cnicin (CNI) from C. benedicta leaves in a mouse model. Activity was assessed using the ear edema method with croton oil, phenol, capsaicin, and histamine as phlogistic agents. Myeloperoxidase (MPO), N-acetyl-β-D-glucosaminidase (NAG), nitric oxide (NO), t umor necrosis factor α (TNF-α), interleukin 6 (IL-6), and histopathology were assessed as markers of edema/inflammation. Interaction profiles between CNI and cyclooxygenase-1 and -2, induced nitric oxide synthase, and glucocorticoid receptor were examined with molecular docking. Twenty-four h after induction of inflammation, ECB and CNI treatments decreased the thickness and weight of ears by 39.59%– 94.72%. MPO, NAG, NO, TNF-α, and IL-6 levels were also reduced. Histopathological, t...
The present study evaluated the total phenolic and flavonoid contents and the antioxidant and cyt... more The present study evaluated the total phenolic and flavonoid contents and the antioxidant and cytotoxic properties of Eremanthus erythropappus. Dried and powdered of E. erythropappus leaves were extracted with ethanol by static maceration followed by partition to obtain the hexane, dichloromethane, ethyl acetate, and butanol fractions. Phytochemical screening and the total phenolic and flavonoid contents were determined. The antioxidant activity was evaluated by DPPH, reducing power of Fe +3 and β-carotene/linoleic acid assays. The cytotoxic test was performed by brine shrimp lethality bioassay. Tannins, flavonoids, coumarins, terpenoids and steroids and saponins were detected in the ethanol extract and fractions. In these samples, the total phenolic and flavonoid contents ranged from 0.98 ± 0.15 to 16.27 ± 0.23 g/100 g and 0.84 ± 0.06 to 5.09 ± 0.22 g/100 g, respectively. The ethanol extract and fractions showed antioxidant effect as free radical scavengers and inhibitors of lipid ...
Information behavior (IB) research in LIS and Health Information Seeking Behavior (HISB) in Healt... more Information behavior (IB) research in LIS and Health Information Seeking Behavior (HISB) in Health Medicine are two subject areas of research that have matured in the past few decades. This research aimed to compare these two research areas using a bibliometric approach. To conduct this study two distinct datasets were created using the Scopus database: a) bibliographic records of IB in the LIS domain, and b) bibliographic records of the HISB domain. The bibliometric analysis was performed according to the following criteria: published papers, citations, journal articles, author keywords, unique words in the title, words preceding "information" in the title, words preceding "study" in the title, and author keywords along with index keywords. As a result, the major differences in the two IB research areas were evident in terms of definitions, main focus, and general demographic groups. These varying types of differences suggest that researchers of the two areas should have flexibility when examining issues related to IB by considering the context and the unique distinction between the two fields.
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Bioactivities of extracts from Eugenia uniflora L. branches were investigated using in vitro and ... more Bioactivities of extracts from Eugenia uniflora L. branches were investigated using in vitro and vivo tests. Dried and powdered from E. uniflora branches were exhaustivel y extracted with hexane, ethyl acetate and ethanol by static maceration obtaining the hexane (HE), ethyl acetate (EAE) and ethanol (EE) extracts. Phytochemical scr eening and total phenolic and flavonoid contents were determin ed. The antioxidant activity was assessed by 2,2-di phenyl-1pycrilhydrazin (DPPH) and iron reducing power tests . For evaluate the antinociceptive activity, acetic acid-induced writhing, formalin and hot plate assays were perfor med, while the anti-inflammatory activity by paw ed ema method. Flavonoids, tannins, coumarins, terpenes and sterol s were revealed in the extracts. HE, EAE and EE pre sented expressive levels of total phenolic and flavonoids and a promising antioxidant effect. Doses of HE, EA E or EE (50, 100 or 200 mg/kg) decreased the number of writhes a nd the phases of paw lickin...
Base de dados : LILACS. Pesquisa : 602464 [Identificador único]. Referências encontradas : 1 [ref... more Base de dados : LILACS. Pesquisa : 602464 [Identificador único]. Referências encontradas : 1 [refinar]. Mostrando: 1 .. 1 no formato [Detalhado]. página 1 de 1, 1 / 1, LILACS, seleciona. para imprimir. Fotocópia. Texto completo. experimental, Documentos relacionados. ...
African Journal of Pharmacy and Pharmacology, 2016
The present study investigated the in- vivo and vitro bioactivities of the essential oil of Dugue... more The present study investigated the in- vivo and vitro bioactivities of the essential oil of Duguetia lanceolata branches (EODL) and caryophyllene oxide (CO). The chemical composition of EODL was analyzed by GC and GC/MS. Acetic acid-induced writhing, formalin, hot plate and tail-immersion methods were used to evaluate the antinociceptive effect in mice. Anti-inflammatory activity was tested by carrageenan-induced paw edema and pleurisy in rats, and in mouse models of acute ear inflammation induced by croton oil and arachidonic acid. 2,2-Diphenyl-1-picrylhydrazyl (DPPH), reducing power and 𝛽-carotene bleaching assays were used to determine the antioxidant bioactivity. -Elemene, caryophyllene oxide and -eudesmol were the major components found in EODL. When tested, EODL and CO reduced the number of writhes and both phases of the time of paw licking, while the reaction time on a hot plate and the pain latency duration by the tail-immersion assay were increased. EODL and CO were effec...
Acta Scientiarum. Health Sciences, 2020
Rutin is a flavonoid used in clinical practice to treat capillary fragility and prevent bleeding ... more Rutin is a flavonoid used in clinical practice to treat capillary fragility and prevent bleeding due to its wide variety of pharmacological actions, including antioxidant, anti-inflammatory, anti-allergic, antiproliferative, and anticarcinogenic activities. In this study, the biopharmacotechnical and physical properties of film-coated tablets containing rutin marketed in drugstores were evaluated. Using samples from three batches called A, B and C, we determined the average weight, disintegration time, hardness, content and dissolution profile, and kinetics of the tablets. The samples demonstrated average weight of 457.45 ± 12.32 to 449.15 ± 8.95 mg; disintegration time, 30.17 ± 2.14 to 15.17 ± 2.14 min; hardness, 1.92 ± 0.55 to 1.69 ± 0.36 Kgf; and rutin content, 18.34 ± 1.21 to 15.66 ± 1.29 mg. After 90 min, the dissolution profile showed 52.65, 41.80, and 79.2% for A, B, and C, respectively. The results imply that the non-conformities of the tested products can significantly comp...
Oxidative Medicine and Cellular Longevity, 2019
Kalanchoe brasiliensisCambess. is a native Brazilian plant popularly known as “saião”, and the ju... more Kalanchoe brasiliensisCambess. is a native Brazilian plant popularly known as “saião”, and the juice of its fresh leaves is traditionally used to treat several disorders, including inflammatory and infectious processes such as dysentery. The goals of this study were to characterize the phytochemical composition and investigate the antioxidant activity, the antibiotic effect, and the mode of action againstSalmonellaof the hydroethanolic extracts fromK. brasiliensisleaves collected in the summer and spring Brazilian seasons. These extracts had their chemical composition established by high-performance liquid chromatography with diode-array detection. Total phenolic and flavonoid contents were spectrophotometrically determined. 2,2-Diphenyl-1-picryl-hydrazyl radical scavenging, phosphomolybdenum reducing power andβ-carotene bleaching assays were carried out to evaluate the antioxidant capacity. Antibiotic potential was assessed by minimal inhibitory concentration against 8 bacterial AT...
Oxidative medicine and cellular longevity, 2018
This study investigated the and in silico biological properties of the methyl chavicol (MC) and i... more This study investigated the and in silico biological properties of the methyl chavicol (MC) and its analogue 2-[(4-methoxyphenyl)methyl]oxirane (MPMO), emphasizing the antioxidant and antilipase effects. MPMO was synthesized from MC that reacted with -chloroperbenzoic acid and, after separation and purification, was identified by H and C NMR and GC-MS. The antioxidant activity was investigated by DPPH, cooxidation -carotene/linoleic acid, and thiobarbituric acid assays. With the use of colorimetric determination, the antilipase effect on the pancreatic lipase was tested, while the molecular interaction profiles were evaluated by docking molecular study. MC (IC = 312.50 ± 2.28 g/mL) and MPMO (IC = 8.29 ± 0.80 g/mL) inhibited the DPPH free radical. The inhibition of lipid peroxidation (%) was 73.08 ± 4.79 and 36.16 ± 4.11 to MC and MPMO, respectively. The malonaldehyde content was significantly reduced in the presence of MC and MPMO. MC and MPMO inhibited the pancreatic lipase in 58.1...
Journal of Medicinal Plants Research, 2017
Palicourea rigida Kunth (Rubiaceae), also called "bate-caixa" or "douradão", has been used as ant... more Palicourea rigida Kunth (Rubiaceae), also called "bate-caixa" or "douradão", has been used as antihypertensive, antiulcerogenic, anti-inflammatory and analgesic by traditional communities. Pharmacological potential of the ethanol extract from P. rigida (EEPR) and two quercetin derivatives were investigated. Using the high performance liquid chromatography (HPLC) assay, EEPR was analyzed. Phenolic contents (total phenolic and flavonoids) were quantified by spectrophotometric methods. 2,2-diphenyl-1-pycrilhydrazil (DPPH), iron reducing power and β-carotene/linoleic acid bleaching tests were applied to estimate the antoxidant capacity of EEPR. Nociception (acetic acidinduced writhing, formalin and hot plate) and inflammation (carrageenan-induced paw edema and pleurisy) assays were performed. Molecular docking was used to measure the interactions' profiles of ligands (rutin and quercetin) and cyclooxigenases (COX-1 and COX-2). HPLC analysis identified rutin and quercetin derivatives. Expressive levels of total phenolic and flavonoids and a promising antioxidant effect were measured. EEPR, rutin and quercetin reduced the abdominal contortions. EEPR was effective against both phases of formalin, while rutin and quercetin inhibited the second phase. The latency time on hot plate significantly increased after treatment with EEPR. Inflammatory parameters (paw edema, exudate volume and leukocyte infiltrate) were diminished by EEPR, rutin and quercetin. The molecular docking showed that rutin and quercetin are capable of complexing with COX-1 and COX-2 favorably through physical-chemical interactions. The results suggest that EEPR showed a relevant pharmacological potential, which may be related to action of rutin and quercetin derivatives.
International journal of molecular sciences, 2016
Vernonia polyanthes Less. (Asteraceae), popularly known as "assa-peixe", is a plant spe... more Vernonia polyanthes Less. (Asteraceae), popularly known as "assa-peixe", is a plant species used in Brazilian traditional medicine for the treatment of cutaneous damage, cicatrization, inflammation, and rheumatism. Based on these ethnopharmacological findings, the current study evaluated the topical anti-inflammatory effects of the hexane (HEVP) and ethyl acetate (EAEVP) extracts from V. polyanthes leaves in experimental models of skin inflammation. Chemical characterization was carried out by HPLC-UV/DAD analysis. Anti-inflammatory activity was evaluated using Croton oil-, arachidonic acid (AA)-, phenol-, ethyl phenylpropiolate (EPP)-, and capsaicin-induced ear edema models in mice. Histopathological evaluation and measurements of myeloperoxidase (MPO) and N-acetyl-β-d-glucosaminidase (NAG) enzymes were also performed. Rutin, luteolin, and apigenin were identified in EAEVP. Topically applied HEVP and EAEVP significantly (p < 0.05, p < 0.01 or p < 0.001) reduced e...
Anais da Academia Brasileira de Ciências, 2016
The present study was designed to investigate the antioxidant, antinociceptive and anti-inflammat... more The present study was designed to investigate the antioxidant, antinociceptive and anti-inflammatory activities of the ethanol extract from Ageratum fastigiatum branches. Phytochemical screening and total phenol and flavonoid contents were determined. The antioxidant activity was assessed by 2,2-diphenyl-1-pycrilhydrazin (DPPH) and iron reducing power methods. The antinociceptive effect was evaluated using the acetic acid-induced writhing, formalin, hot plate and tail immersion assays; while the carrageenan-induced paw edema and pleurisy tests were performed to examine the anti-inflammatory activity against acute inflammation. The extract revealed the presence of flavonoids, tannins, coumarins, terpenes, sterols and saponins. Expressive levels of total phenols and flavonoids and a promising antioxidant effect were quantified. At the doses of 50, 100 and 200 mg/kg, the extract inhibited the writhing, reduced both phases of paw licking time and increased the reaction time on the hot p...
O presente estudo investigou as atividades antinociceptiva e antiedematogênica do extrato etanóli... more O presente estudo investigou as atividades antinociceptiva e antiedematogênica do extrato etanólico das folhas de J. princeps através dos testes de contorções abdominais, formalina, placa quente e edema de pata induzido por carragenina. O extrato etanólico reduziu (p < 0,05) as contorções abdominais (100 mg/kg = 55,75±1,29 e 200 mg/kg = 47,75±1,35) quando comparado ao grupo controle (67,25±1,51). Ambas as fases do teste de formalina foram inibidas (p < 0,05): 1ª fase (50 mg/kg = 79,50±1,12; 100 mg/kg = 69,37±1,03 e 200 mg/kg = 56,75±1,95) e 2ª fase (50 mg/kg = 86,50±1,22; 100 mg/kg = 69,62±1,66 e 200 mg/kg = 49,37±1,50). Após 90 min de tratamento, o limiar nociceptivo dos animais quando avaliados no modelo de placa quente foi aumentado de forma significativa com o extrato nas doses de 100 mg/kg (8,12±0,48) e 200 mg/kg (10,25±0,45) quando comparados com o grupo controle (6,62±0,46). Após 3 h de aplicação da carragenina, a dose de 200 mg/kg (0,43±0,02) reduziu o edema de pata em...
Revista Brasileira de Farmacognosia, 2008
RESUMO: Ageratum fastigiatum (Gardn.) R. M. King et H. Rob. (Asteraceae), conhecido como "matapas... more RESUMO: Ageratum fastigiatum (Gardn.) R. M. King et H. Rob. (Asteraceae), conhecido como "matapasto", é uma planta usada na medicina popular como cicatrizante e antimicrobiano. O objetivo deste trabalho foi realizar caracterização morfoanatômica da folha, dos ramos e da raiz de A. fastigiatum. Secções transversais e paradérmicas, coradas com safranina/azul de astra e azul de toluidina foram analisadas ao microscópio. As folhas são alternas ou fasciculadas; a infl orescência é constituída de capítulos; as fl ores com corola lilás; ausência de pápus. A raiz apresenta crescimento secundário na zona de ramifi cação com estruturas secretoras. O caule possui epiderme uniestratifi cada, estômatos, tricomas tectores simples e tricomas glandulares capitados. O pecíolo exibe contorno côncavo-convexo e a epiderme é unisseriada. A lâmina foliar é anfi estomática e o mesófi lo dorsiventral. A epiderme possui células de contorno sinuoso e os estômatos são do tipo anomocítico. Os caracteres morfoanatômicos permitiram estabelecer parâmetros para identifi cação do A. fastigiatum.
Revista Brasileira de Farmacognosia, 2003
Resumo As plantas denominadas de ruibarbo sintetizam antraquinonas e taninos, que são responsávei... more Resumo As plantas denominadas de ruibarbo sintetizam antraquinonas e taninos, que são responsáveis pelos efeitos laxante e adstringente, respectivamente. Análises da qualidade de cinco matérias-primas de ruibarbo foram realizadas a fim de detectar adulterações. As reações para antraquinonas e taninos foram positivas. Os constituintes rapônticos foram observados na amostra de Rheum palmatum (2). As medidas de cinzas totais para Rheum palmatum (2) e Ferraria cathartica estão acima do esperado. Conforme resultados, as amostras de Rheum palmatum (2) e Ferraria cathartica devem estar adulteradas.
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Papers by Orlando Vieira de Sousa