Papers by Oliver Stojkovic
![Research paper thumbnail of [Comparison of the number of CAG repeats in the gene for androgen receptors in a control Yugoslav population and in patients with schizophrenia]](https://a.academia-assets.com/images/blank-paper.jpg)
PubMed, Aug 10, 2000
Introduction: Dynamic mutations were recently discovered causing hereditary non polyposis colon c... more Introduction: Dynamic mutations were recently discovered causing hereditary non polyposis colon cancer. Soon almost 15 hereditary neurological diseases were described caused by the expansion of trinucleotide repeats in target genes. These mutations are unstable: the number of trinucleotide repeats is increasing from generation to generation. These mutations do not obey Mendelian low. There is a positive correlation between the number of repeats and the severity of clinical symptoms, as well as with the age of onset. This fact explains the genetical basis of anticipation. Since schizophrenia is showing non-Mendelian way of inheritance and anticipation, it is believed that it might be caused by trinucleotide repeats in some gene(s). We analysed the number of CAG triplets in the gene for androgen receptor (AR) where expansions are causing spinal and bulbar muscular atrophy in healthy and schizophrenic subjects. The aim of this study was to see if the androgen receptor gene in schizophrenic patients shows instability in the number of trinucleotide repeats. Patients and methods: In healthy Yugoslav population we analysed 85 X chromosomes from 52 non-related individuals (33 females and 19 males) from healthy Yugoslav population and 84 X chromosomes (41 females and 2 males) from patients with schizophrenia. DNA was isolated from peripheral blood leukocytes and used for further PCR amplification of the segment of AR gene containing CAG repeats. The exact number of these repeats was determined by electrophoresis on a 5% denaturing polyacrilamide gel stained by silver. Results: In healthy Yugoslav population we detected 16 different AR alleles in which the number of CAG triplets was from 14 to 29. The most common alleles were with 23 repeats (14.1%) and with 22 repeats (12.9%). The average number of CAG triplets per allele was 20.91. In patients with schizophrenia we detected 13 AR different alleles. The number of triplets was from 17 to 30. The most common allele was with 22 repeats (25%). The average number of CAG triplets per allele was 22.1.
International Journal Of Legal Medicine, Dec 20, 2019
Plethora of drugs and toxic substances is metabolized by cytochrome P450 enzymes (CYP450). These ... more Plethora of drugs and toxic substances is metabolized by cytochrome P450 enzymes (CYP450). These enzymes are coded by highly variable genes abundant with single nucleotide variants (SNVs) and small insertions/deletions (indels) that affect the functionality of the enzymes, increasing or decreasing their activity. CYP genes genotyping, followed by haplotype inference, provides substrate specific metabolic phenotype prediction. This is crucial in pharmacogenetics and applicable in molecular autopsy. However, high number of alleles in CYP450 superfamily and interethnic variability in frequency distribution require precise gene panel customization. To estimate informativeness of SNVs and alleles in CYP gene families 1, 2, and 3, associated with metabolic alterations, 500 unrelated individuals from 5 regions of Serbia were genotyped using TaqMan assays to determine frequencies of CYP2C9 *2 and *3, CYP2C19 *2 and *17 alleles, four variants in CYP2D6 ( rs3892097 , rs1065852 , rs28371725 , rs28371706 ) gene, and CYP3A4*1B allele. In addition, CYP1A1 rs4646903 and rs1048943 (m1 and m2) variants were genotyped by RFLP. Our results showed that frequencies of tested variants in Serbian population corresponded to general European population and somewhat differed from neighboring populations. SNV rs1065852 , the main contributor to non-functional CYP2D6 *4 , significantly departed from Hardy-Weinberg equilibrium. With the exception of rs28371706 in CYP2D6 and rs2740574 in CYP3A4 , which were very rare in our sample, all other tested variants in CYP2 family are informative and appropriate for pharmacogenetic testing, molecular autopsy, and medico-legal genetic analyses.
American journal of medical genetics, 2000
Medicinski Podmladak, 2016
Since the renin-angiotensin-aldosterone system (RAAS) was originally described, it has become one... more Since the renin-angiotensin-aldosterone system (RAAS) was originally described, it has become one of the best described hormonal systems, especially regarding the fact that it plays an important role in regulating blood volume and systemic vascular resistance, and thus indirectly influencing blood pressure (BP). On the other hand, arterial hypertension is one of the most pertinent disorders which plays an important role, not only in the progression of renal failure, but also represents a risk factor for the occurrence of end stage renal disease. Several epidemiological studies pointed out the fact that genetic predisposition accounts for about 30% of the BP variability. Up to date, there are several RAAS genes that may have effect in long-term BP control, but ACE is the most important and the most thoroughly examined. In this review, we present available data regarding the influence of gene polymorphisms of ACE on its function, within the RAAS related BP regulation. Therefore, by specially describing all its potential physiological roles, it will likely offer a new insight in the renal regulation of the BP, along with its other, not less important, roles.
Evolution, Dec 1, 1998
In this study we examined the direct and correlated responses for fast and slow preadult developm... more In this study we examined the direct and correlated responses for fast and slow preadult development time in three laboratory populations of the bean weevil (Acanthoscelides obtectus). The first population ("base," B) has experienced laboratory conditions for more than 10 years; the second ("young," Y) and the third ("old," 0) populations were selected for early and late reproduction, respectively, before the onset of the present experiments. All three populations are successfully selected for both fast and slow preadult development. The realized heritabilities are very similar in all populations, suggesting a similar level of the additive genetic variance for preadult development. We studied the correlated responses on the following life-history traits: egg-to-adult viability, wet body weight, early fecundity, late fecundity, total realized female fecundity, and adult longevity. All life-history traits examined here, except for the egg-to-adult viability, are affected by selection for preadult development in at least in one of the studied populations. In all three populations, beetles selected for slow preadult development are heavier and live longer than those from the fast-selected lines. The findings with respect to adult longevity are unexpected, because the control Y and 0 populations, selected for short-and long-lived beetles, respectively, do not show significant differences in preadult development. Thus, our results indicate that some kind of asymmetrical correlated responses occur for preadult development and adult longevity each time that direct selection has been imposed on one or the other of these two traits. In contrast to studies with Drosophila, it appears that for insect species that are aphagous as adults, selection for preadult development entails selection for alleles that also change the adult longevity, but that age-specificselection (applied in the Y and 0 populations) mostly affects the alleles that have no significant influence on the preadult development. Implications of these findings on the developmental and evolutionary theories of aging are also discussed.
Medicinski Pregled, 2022
Introduction. Poor graft function is one of the most severe complications after allogeneic hemato... more Introduction. Poor graft function is one of the most severe complications after allogeneic hematopoietic stem cell transplantation, which manifests as pancytopenia/cytopenia in the blood count, with the presence of complete or incomplete donor chimerism. There are three entities of graft weakness: 1. poor graft function: pancytopenia with complete donor chimerism, 2. graft failure: pancytopenia with incomplete, i.e., mixed donor chimerism and 3. graft rejection: progressive decline of donor chimerism. Definition. Poor graft function is diagnosed as pancytopenia (hemoglobin < 70 g/L, absolute neutrophil count < 0.5 x 10 9 /L, platelets < 20 x 10 9 /L) for 3 consecutive days from D+28, excluding the presence of severe graft versus host disease and relapse, with complete donor chimerism in poor graft function, and incomplete in graft failure. Risk factors and therapeutic principles. The most common risk factors for poor graft function are a small dose of CD34+ hematopoietic stem cells in the transplant, graft versus host disease, cytomegalovirus infection, the presence of donor-specific antibodies, high serum ferritin, i.e., iron overload, as well as splenomegaly. Pathogenetic mechanisms in the development of poor graft function are still not fully elucidated. The role of the microenvironment of the patient's bone marrow is also important, as well as disorders of the immune system Therapeutic options for overcoming this complication include using selected "stem cell boost", mesenchymal stem cells, and newer medical agents (N-acetyl cysteine, atorvastatin, thrombopoietin receptor agonists). Conclusion. The type of poor function of the graft is defined in relation to the percentage of donor chimerism, and is necessary for planning further treatment strategy.
Genetika
The aim of study was to investigate distribution of ACE and ACTN3 gene polymorphisms in young fem... more The aim of study was to investigate distribution of ACE and ACTN3 gene polymorphisms in young female footballers and to test association of common gene polymorphisms with body composition, arterial blood pressure and ECG screening variables. A group of 45 white, healthy, adolescent female elite footballers (FG) and 60 sedentary female controls (CG) enrolled in this study. HRM method has been developed to differentiate between variant alleles of ACE and ACTN3 genes. No significant difference was found in the ACE and ACTN3 genotypes or allele frequencies distribution between FG and CG (p>0.05). Also, neither insertion in the ACE gene, nor nonsense mutation in the ACTN3 gene had a significant effect on resting BP and ECG parameters. Cardiovascular adaptation to intensive physical activity in FG is manifested as lowered resting systolic and diastolic blood pressure (lower 18 and 11 percentiles, respectively). Footballers with ACE DD and ACTN3 XX polymorphisms had higher values of Sok...
Medical review
Introduction. Poor graft function is one of the most severe complications after allogeneic hemato... more Introduction. Poor graft function is one of the most severe complications after allogeneic hematopoietic stem cell transplantation, which manifests as pancytopenia/cytopenia in the blood count, with the presence of complete or incomplete donor chimerism. There are three entities of graft weakness: 1. poor graft function: pancytopenia with complete donor chimerism, 2. graft failure: pancytopenia with incomplete, i.e., mixed donor chimerism and 3. graft rejection: progressive decline of donor chimerism. Definition. Poor graft function is diagnosed as pancytopenia (hemoglobin < 70 g/L, absolute neutrophil count < 0.5 x 109/L, platelets < 20 x 109/L) for 3 consecutive days from D+28, excluding the presence of severe graft versus host disease and relapse, with complete donor chimerism in poor graft function, and incomplete in graft failure. Risk factors and therapeutic principles. The most common risk factors for poor graft function are a small dose of CD34+ hematopoietic stem c...
Animals
We explored the cryptic speciation of the Nannospalax leucodon species complex, characterised by ... more We explored the cryptic speciation of the Nannospalax leucodon species complex, characterised by intense karyotype evolution and reduced phenotypic variability that has produced different lineages, out of which 25 are described as chromosomal forms (CFs), so many cryptic species remain unnoticed. Although some of them should be classified as threatened, they lack the official nomenclature necessary to be involved in conservation strategies. Reproductive isolation between seven CFs has previously been demonstrated. To investigate the amount and dynamics of genetic discrepancy that follows chromosomal changes, infer speciation levels, and obtain phylogenetic patterns, we analysed mitochondrial 16S rRNA and MT-CYTB nucleotide polymorphism among 17 CFs—the highest number studied so far. Phylogenetic trees delineated 11 CFs as separate clades. Evolutionary divergence values overlapped with acknowledged higher taxonomic categories, or sometimes exceeded them. The fact that CFs with higher...
British Journal of Sports Medicine, 2016
Objectives This study aimed to evaluate the association of ACE insertion/deletion (I/D) and ACTN3... more Objectives This study aimed to evaluate the association of ACE insertion/deletion (I/D) and ACTN3 R577X polymorphisms with resting, maximal and recovery blood pressure (BP) and left ventricular hypertrophy measured by left ventricular mass index (LVMI) in elite athletes. Methods A group of 107 white, healthy, elite male athletes, aged between 20 and 35 yr, enrolled in this study. All of them participated either in sprint/power sports (n = 17), endurance (n = 36) or in mixed sports (n = 54). HRM method has been developed to differentiate between variant alleles of ACE and ACTN3 genes (Figures 1 and 2). Results No significant difference was found in the ACE and ACTN3 genotypes or allele frequencies distribution between sprint/power, endurance or mixed sports athletes (p > 0.05). Also, neither insertion in the ACE gene, nor nonsense mutation in the the ACTN3 gene had a significant effect on resting and maximal BP. Sport activity, but not the analysed polymorphisms influence resting diastolic, maximal systolic and recovery systolic BP (p < 0.05). According to ACE dominant model the two-way ANOVA indicated a significant relation between the genotype (II+ID vs. DD) and the type of sport in systolic BP recovery (p = 0.006 and p = 0.03, respectively), but not the significance of its interaction (p > 0.05). In relation to maximal BP, decrease in systolic BP at 3 min of recovery was higher in ACE II/ID group compared to ACE DD in all sport groups (endurance, sprint/power and mixed: 78 vs. 86%, 79 vs. 83%, 67 vs. 78%, respectively, p < 0.05). In contrast, the two-way ANOVA indicated a significant interaction between the ACE genotype (II+ID vs. DD) and the type of sport in LVMI (p = 0.02). LVMI was significantly higher in ACE II/ID group compared to ACE DD in all sport groups (endurance, sprint/power and mixed: 107.5 vs. 104.3 g/m2; 104.6 vs. 103.6 g/m2; 113.7 vs. 89.72 g/m2, p < 0.05). No significant difference was found in the ACTN3 genotypes or allele frequencies distribution between the three groups as regards resting, maximal and recovery BP and LVMI (p > 0.05). Conclusion These data show that LVMI as a marker of LVH depends significantly on the interaction between ACE polymorphism and the type of sport activity. References Gunel T, Gumusoglu E, Hosseini MK, Yilmazyildirim E, Dolekcap I, Aydinli K. Effect of angiotensin I-converting enzyme and α-actinin 3 gene polymorphisms on sport performance. Mol Med Rep 2014 Apr;9(4):1422–6. Ma F, Yang Y, Li X, Zhou F, Gao C, Li M, Gao L. The association of sport performance with ACE and ACTN3 genetic polymorphisms: a systematic review and meta-analysis. PLoS One 2013;8(1):e54685. Saber-Ayad MM, Nassar YS, Latif IA. Angiotensin-converting enzyme I/D gene polymorphism affects early cardiac response to professional training in young footballers. J Renin Angiotensin Aldosterone Syst 2014;15(3):236–42. Abstract O-23 Figure 1 Abstract O-23 Figure 2
Forensic Science International, Apr 1, 2008
Seventeen Y-chromosomal STR (short tandem repeat) loci were analyzed in a group of 185 healthy un... more Seventeen Y-chromosomal STR (short tandem repeat) loci were analyzed in a group of 185 healthy unrelated male individuals (n=185) from the population of Serbian province of Vojvodina. After minimal haplotype STR loci analysis we observed 129 different haplotypes. The most frequent haplotype was found in 13 copies, and total haplotype diversity was 99.11%. After analysis of additional eight Y-STR loci (DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635 and YGATAH4) there were 176 different haplotypes observed, out of which 168 appeared in single copies, and 7 haplotypes appeared twice. The most frequent haplotype was found in three copies. The haplotype diversity (99.94%) and discrimination capacity (95.13%) were calculated. Comparisons were made with previously published haplotype data on neighbouring population samples and significant differences were demonstrated at DYS19, DYS389II and DYS393 loci. Pairwise comparison of populations revealed that our sample was significantly different only from Hungarian sample (RST=23.98%, p=0.0091).
Psychiatric Genetics, 2001
6.6% in the group of non-triplet diseased patients; P-0.001, Fisher's exact test. This is consist... more 6.6% in the group of non-triplet diseased patients; P-0.001, Fisher's exact test. This is consistent with our previous findings in HD patients. The absence of this association in non-triplet diseases as well as in healthy controls could indicate a possible role of this SCA1 allele with 31 repeats in triplet diseases. Here we discuss a possible role of the SCA1 region in pathological trinucleotide repeat expansions. Psychiatr Genet 11:201
Srpski arhiv za celokupno lekarstvo
In 1993 the gene responsible for Huntington's disease (IT15) was isolated [5]. It was mapped ... more In 1993 the gene responsible for Huntington's disease (IT15) was isolated [5]. It was mapped to the tip of the short arm of chromosome 4 and within its coding sequence, near the 5' end, it contained a certain number of trinicleotide (CAG)n (cytosine-adenine-guanine) repeats (Figure 1). This gene codes for a protein (348 kd) called "huntington" that is widely expressed, and its sequence is not related to any protein [6]. The normal range of (CAG)n repeat numbers within IT15 was reported to be between 6 and 37 [6]. Mutation responsible for Huntington's disease implied expansion of (CAG)n repeats: in patients with Huntington's disease the pathologic range was determined to be between 35 and 121 repeats [7-10]. In this study we correlated the age at onset, rate of progression and initial symptoms of Huntington's disease with the number of trinucleotide (CAG)n repeats in IT15. DNA was isolated from peripheral blood leukocytes of patients fulfilling clinical ...
Forensic Science International: Genetics, 2017
Highlights The PowerQuant kit was tested as a predictor of autosomal STR typing success Quant... more Highlights The PowerQuant kit was tested as a predictor of autosomal STR typing success Quantity and quality of DNA was examined on ancient and WWII bone samples Bone extracts with no PowerQuant quantitation results did not produce any profile STR profiles were generated from bones with simultaneously detected short and long targets The PowerQuant kit can be used as a predictor of autosomal STR typing success
The Tohoku Journal of Experimental Medicine, 2017
Physiological adaptations to various types of prolonged and intensive physical activity, as seen ... more Physiological adaptations to various types of prolonged and intensive physical activity, as seen in elite athletes from different sports, include changes in blood pressure (BP) response to acute exercise. Also, functional polymorphisms of the angiotensin I converting enzyme (ACE) and alfa-actinin-3 (ACTN3) genes are shown to be associated with BP parameters changes, both in athletes and sedentary population. In this study, an Alu insertion (I)/deletion (D) polymorphism in ACE gene, as well as nonsense mutation in the gene encoding ACTN3 have been scored in 107 elite Serbian athletes classified according to their sporting discipline to power/sprint (short distance runners/swimmers), endurance (rowers, footballers, middledistance swimmers) or mixed sports (water polo, handball, volleyball players). Presence of nonfunctional allele in ACTN3 is associated with significantly increased maximal systolic BP (SBPmax, p = 0.04). Athletes with Alu insertion in ACE had significantly (p = 0.006) larger decline of systolic BP after 3 minutes of recovery (SBPR3), calculated as the percentage of maximal SBP response during exercise stress testing. Concomitant presence of non-functional variant in ACTN3 gene decreased this beneficiary effect of ACE mutation on SBPR3. Long term enrollment in power/sprint sports significantly increased resting diastolic BP (DBPrest: 74 mmHg) and SBPmax (197 mmHg) and improved SBPR3 (74.8%) compared to enrolment in endurance (72 mmHg; 178mmHg; 81.1%) and mixed sports (69 mmHg; 185 mmHg; 80.0%). Lack of the effect of genotype by sport interaction on BP parameters suggests that the long-term effects of different disciplines on BP are not mediated by these two genes.
processing of information, which is important for learning, reasoning and comprehension. Although... more processing of information, which is important for learning, reasoning and comprehension. Although the model and components of WM are still debated, there is a consensus that WM is essential for the development of many language-related traits including speech production and processing and language learning. It has been suggested that the rare trait of backward-speaking is linked to WM. Two strategies of word reversal were reported: (1) reversal according to the phonetic structure of the words (speech sounds) or (2) reversal according to their spelling (letters). Multidisciplinary investigation of backward-speech trait suggests a link between RIC3, RIPK1, ZBED5 and working memory
International Journal of Forensic Science & Pathology, 2015
Frontiers in Medicine, 2021
In Europe, the first case of coronavirus disease (COVID-19) and the first COVID-19-related death ... more In Europe, the first case of coronavirus disease (COVID-19) and the first COVID-19-related death were reported in France on January 24th and February 15th, 2020, respectively. Officially, the first case of COVID-19 infection in the Republic of Serbia was registered on March 6th. Herein, we presented the first case of retrospective detection of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in the post-mortem-obtained vitreous humor (VH), which took place on February 5th, 2020. This is the first death in Europe proven to be caused by COVID-19 by means of post-mortem histopathological and molecular analyses. Based on this finding, it appears that SARS-CoV-2 has been spreading faster and started spreading much earlier than it had been considered and that COVID-19 was probably the cause of the much-reported pneumonia of unknown origin in January and February 2020.
Uploads
Papers by Oliver Stojkovic