Papers by Oriela Pino Pérez
The essential oils have been considered an important alternative to sustainable management of pes... more The essential oils have been considered an important alternative to sustainable management of pests in agriculture due to their recognized antimicrobial activity against several pests of important economic crops. The aim of this study was to identify the potential of essential oils of Thymus vulgaris L., Pimpinella anisum L., Citrus sinensis (L.) Osbeck., Ocimum basilicum L., Curcuma longa L., Ruta chalepensis L. and Melaleuca quinquenervia (Cav) S.T. Blake as candidates for the development of new antibacterials. Essential oils were extracted by hydrodistillation using a Clevenger-type equipment and their biological evaluation against Pectobacterium carotovorum subsp. carotovorum (Jones) Waldee emends. Hauben et al. was carried out by agar diffusion method. The minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) of the most active essential oil was carried out by the serial dilution method and its chemical composition was determined by GC / MS. The MIC and MBC of the main compound from the most active essential oil were determined by the serial dilution method. Among the tested oils, anise and white basil essential oils showed weak activity, whereas the essential oil of thyme showed strong antibacterial activity brought to you by CORE View metadata, citation and similar papers at core.ac.uk
Cultivos Tropicales, Dec 1, 2014
The study was conducted in order to investigate the possible competition between Helianthus annus... more The study was conducted in order to investigate the possible competition between Helianthus annus L. sunflower) over Solanum lycopersicum L. (tomato) associated in field conditions of Guantanamo´s province mountain agro ecosystem, Cuba, during September to December period, for two years. Four treatments were studied: tomato monoculture; sunflower monoculture; tomato planted simultaneously with sunflower; and tomato planted ten days before sunflower. Crops were established in stripes of 150 m 2. The most efficient treatment (tomato planted ten days before sunflower) was repeated during the second year, together with monoculture treatments. Land Use Equivalent Index (IET), based in crop yields and considering the total policulture system period (ATER) were calculated. The greatest tomato production was found in tomato planted ten days before sunflower treatment; as IET value was greater than one, economical benefits could be attributed related to monoculture, but ATER reached values of one, indicating neutrality. Nevertheless, taking into account the ecological benefits the result constitutes an option among functional diversity due to the fact that association improves ecosystem balance because in it, two food crops were obtained, increases soil use efficiency, and probably also it estimulate beneficial insect fauna.
The International Journal of Cuban Studies, Jun 1, 2008
ABSTRACT Tropical flora constitutes a rich source of substances that can be applied directly, or ... more ABSTRACT Tropical flora constitutes a rich source of substances that can be applied directly, or as compounds, to the development of new pest control agents and pharmaceuticals. Partly due to its great diversity, Cuban flora has not yet been closely studied as a potential source of chemical pesticides. Mass screening of a variety of plants is needed to determine the presence of bioactive compounds which could be used instead of commonly adopted agrochemicals and drugs. This study from the Centre for Plant Protection in Havana has focused on a selection of plants as potential sources of new natural products for botanical pesticides and medicines. For the first time in Cuba, this research has been carried out using a refined bio-assay. Extracts from plants belonging to the Anacardiaceae, Asteraceae, Clusiaceae, Myrtaceae, Piperaceae, Anonaceae and Poaceae families were evaluated in terms of their effectiveness against bacteria, fungi, insects, mites and Artemia (brine shrimp). Plants from four of these families displayed activity and were then selected for further fractionation and purification. From a social and economic perspective, the identification of local candidates for developing phytosanitary products offers greater safety and efficiency for Cuban agriculture. This research could eventually assist other developing countries in developing national policies for agricultural management.
Industrial Crops and Products, 2015
Gastrointestinal nematodes are an important problem in the small ruminant production and their co... more Gastrointestinal nematodes are an important problem in the small ruminant production and their control nearly exclusively depends on commercial anthelmintics. However, parasite resistance to those chemical formulations is an increasing global problem, so new plant-derived compounds are being studied for their potential use against gastrointestinal nematodes. Citrus sinensis and Melaleuca quinquenervia essential oils were evaluated against Haemonchus contortus Embrapa2010 resistant isolate, through the egg hatch test (EHT) and larval development test (LDT) at concentrations ranging from 0.02 mg/mL to 50 mg/mL and from 0.04 mg/mL to 3.12 mg/mL, respectively. All concentrations, positive controls (thiabendazole in EHT or ivermectin in LDT), and negative controls (2% Tween 80 in EHT or 0.5% DMSO in LDT) were performed in six replicates and in three independent experiments, for a total of 18 repetitions involving approximately 1800 parasites per treatment. Significant differences (P ≤ 0.01) of inhibition percentages were identified by one-way analysis of variance followed by the Tukey test. Inhibitory concentration values (IC 50 and IC 90) were analyzed by the Probit procedure of SAS. The essential oils evaluated showed that C. sinensis presented limonene as major component (96.0%), while M. quinquenervia contained longifolene (32.95%) and 1,8-cineole (25.43%) as major components. In the EHT, the IC 50 and IC 90 of the essential oils were respectively 0.27 and 0.99 mg/mL for C. sinensis and, 1.52 and 5.63 mg/mL for M. quinquenervia. In the LDT, the IC 50 and IC 90 were 0.97 and 2.32 mg/mL for C. sinensis and, 0.44 and 0.94 mg/mL for M. quinquenervia. C. sinensis was five times more effective on eggs than M. quinquenervia. However, it was twice more effective on larvae than C. sinensis, indicating that bioactive essential oils have different modes of action. The results suggest that these compounds are good candidates for nematode control. However, alternative anthelmintics have to be safe to the host, promote parasite control in vivo and not generate residues. So, all these key points need to be elucidated before using any plant extract and/or components.
Revista de Protección Vegetal, 2010
... Artemia spp., y de esta manera se predice su habilidad para producir la muerte de células can... more ... Artemia spp., y de esta manera se predice su habilidad para producir la muerte de células cancerígenas en cultivo de tejidos, matar insectos y ... 19.Mawardi R, Hazar B, Mohd I, Farediah A, Rahman M, Mohd A. Screening of tropical plants for the presence of bioactive compounds ...
Bioresources and Bioprocessing, Jan 4, 2021
The synthesis of silver nanoparticles (SNP) from plants is a simple, fast and environmentally saf... more The synthesis of silver nanoparticles (SNP) from plants is a simple, fast and environmentally safe route. In the present study, the aqueous extract of fresh leaves from Leea coccinea L. was evaluated as a possible source of reducing and stabilizing agents to obtain SNP. The synthesized SNP were characterized by spectroscopic techniques such as UV-visible spectrophotometry and Fourier transform infrared spectroscopy (FTIR), scanning electron and confocal microscopies and the antimicrobial activity against Xanthomonas phaseoli pv. phaseoli was evaluated using agar diffusion methods. The results showed that the evaluated extract was promising for the green synthesis of the SNP, which was visually identified by the formation of a dark-brown complex and the presence of a peak of maximum absorption at 470 nm in a UV-VIS spectrum. FTIR spectrum of SNP showed main characteristic signals of aromatic compounds, carboxylic group among others confirmed by phytochemical screening that made evident the presence of flavonoids, phenols, leucoanthocyanidins, terpenes and steroids groups. Fluorescent SNP with high degree of agglomeration were observed by the microscopical technics used. A promising antibacterial activity of SNP was shown by a zone of microbial growth inhibition. These results suggested the need for going deeper in the physico-chemical characterization and kinetic studies, as well as the biological evaluations to make possible the use of this plant source in the future development of antibacterial formulations for bean seed protection.
RESUMEN: Los aceites esenciales son una alternativa potencial para el control de bacterias fitopa... more RESUMEN: Los aceites esenciales son una alternativa potencial para el control de bacterias fitopatógenas. Los objetivos de este trabajo fueron determinar la composición química del aceite esencial de Piper hispidum Sw. y su actividad antibacteriana sobre Xanthomonas albilineans (Ashby) Dowson y Xanthomonas campestris pv. campestris (Pammel) Dowson. La composición del aceite de las hojas de esta especie, obtenido por hidrodestilación empleando un equipo Clevenger, se investigó por Cromatografía Gaseosa-Espectrometría de Masas (CG/EM). El efecto antibacteriano se evaluó utilizando el método de difusión en agar. El aceite esencial de P. hispidum (rendimiento 0,17%) es una mezcla compleja de 101 componentes. Como componente principal se identificó al trans-α-bisaboleno (11,28%) y se destacan otros como: β-pineno (8,20%), α-pineno (4,76%), allo-aromadendreno (4,92%), (-) espatulenol (3,65%) y L-linalol (3,61%). El aceite presentó una actividad antibacteriana ligera sobre X. campestris pv. campestris y marcada sobre X. albilineans, por lo que puede ser considerado como un candidato para la obtención de antimicrobianos destinados al manejo de esta última bacteria. Palabras clave: aceite esencial, Piper hispidum, Xanthomonas campestris pv. campestris, Xanthomonas albilineans, composición química, actividad antibacteriana. Chemical composition of the essential oil of Piper hispidum Sw. and antibacterial activity against Xanthomonas albilineans (Ashby) Dowson and Xanthomonas campestris pv. campestris (Pammel) Dowson ABSTRACT: The essential oils are a potential alternative to the control of phytopathogenic bacteria. The aims of this work to determine the chemical composition of the essential oil of Piper hispidum Sw. and to established it´s antibacterial activity against Xanthomonas albilineans (Ashby) Dowson and Xanthomonas campestris pv. campestris (Pammel) Dowson. The composition of the leaves essential oil of this plant, obtained by hydrodistillation in a Clevenger-type apparatus, was researched by Gas Chromatography-Mass Spectrometry (GC/MS). The antibacterial effect of the essential oil was evaluated using the agar diffusion method. The essential oil (yield 0,17%) is a complex oil that have trans-αbisabolene (11,28%) as the principal component and there are others like: β-pinene (8,2%), α-pinene (4,76%), allo-aromadendrene (4,92%), (-) spatulenol (3,65%) and L-linalool (3,61%). The oil shown a weak antibacterial activity against X. campestris pv. campestris and strong against X. albilineans, for that it is may consider as a candidate for the management of this last bacterium.
Julius-Kühn-Archiv, 2018
Plants are a source of substances for protection of stored products. The Cuban flora has not yet ... more Plants are a source of substances for protection of stored products. The Cuban flora has not yet been fully studied as a source of pesticides for postharvest protection, partly due to its great diversity. The toxicity of four Cuban plant derivatives against Lasioderma serricorne (F.) and Sitophilus zeamais Motschulsky was investigated. The anti-insect activity of the powders and the essential oil from plants belonging to Asteraceae, Fabaceae and Piperaceae was tested. Mortality and emergence of adult insects and the repellent effect of products were evaluated. Two products derived from Piper aduncum subsp. ossanum, caused high mortality (81,6 and 100%), reduced emergence (27,9 and 0,4%) and exhibited strong repellent activity on L. serricorne. Against S. zeamais, treatments with the highest mortality values were stems of Lonchocarpus punctatus (72,4%), seeds and stems of Canavalia ensiformis (64,9 and 69,9%), and leaves of Tithonia diversifolia (67,2%). The progeny production of S. ...
Los aceites esenciales constituyen un grupo de productos promisorios como plaguicidas; aunque en ... more Los aceites esenciales constituyen un grupo de productos promisorios como plaguicidas; aunque en Cuba representan una alternativa poco explorada como fuente de productos fitosanitarios y su futura aplicación requiere de la ejecución de investigaciones básicas sobre la química y actividad biológica de estos compuestos. Por lo que el objetivo de este trabajo fue establecer el efecto bacteriostático y/o bactericida del aceite de Piper marginatum Jacq. frente a Xanthomonas albilineans (Ashby) Dawson e identificar los componentes responsables de este efecto. El aceite esencial de P. marginatum se obtuvo por hidrodestilación empleando un equipo Clevenger. Se determinó su rendimiento y su composición química se investigó por CG/EM. Se realizó la determinación de la Concentración Mínima Inhibitaria (CMI) y la Concentración Mínima Bactericida (CMB) por el método de las diluciones seriadas frente a X. albilineans. Se identificaron los componentes responsables de la actividad biológica mediant...
ARTÍCULO ORIGINAL Efecto in vitro de aceites esenciales sobre Alternaria solani Sorauer Ya n i s ... more ARTÍCULO ORIGINAL Efecto in vitro de aceites esenciales sobre Alternaria solani Sorauer Ya n i s i a D u a rt e, O ri e la P i n o , D a n a y I n f a n t e , Ya i ma S á n c h ez, M a rí a d el C a rm e n Tra v i e so , B. Martínez
Cuban Journal of Agricultural Science
A completely randomized design was used to determine the principal groups of secondary metabolite... more A completely randomized design was used to determine the principal groups of secondary metabolites, the anti-insect effect against Sitophilus zeamais, and the influence of the powders of the structural organs of the plant (89-1-XIV) on the germination of corn seeds. The treatments were the structural organs of the plant separately: leaf, stalk, and root, and a negative control, with four repetitions each. The anti-insect effect was assessed through the mortality, the emergence, the repellent effect on the adult insect, and the weight loss of the corn grain. For the powders of leaf, there were mortality values of 67.2 % in the adults of S. zeamais. The treatment with the highest mortality had the lowest emergence (38.7 %) and the lowest weight loss of the grain (10.75 %). The best repellent effect was obtained by using the leaf powders. In the percentage of germination, there were only significant differences in the leaves in respect to the rest of the treatments (P < 0.05). It is...
The objective of this research was to determine the potential of essential oils of species of the... more The objective of this research was to determine the potential of essential oils of species of the families Piperaceas, Lamiaceas and Apiaceas to be developed as new pesticides for management of Bemisia tabaci Biotype B. Seven essential oisl were evaluated for their lethal toxicity by contact on B. tabaci adults and their ovicidal effect by a multiple comparison of proportions by the Wald method. In addition, the effect of these oils on the behavior of insects was determined through a simple ANOVA. The essential oils of Piper hispidum Sw, Ocimum gratissimum L., Ocimum basilimum L., Thymus vulgaris L ., and Pimpinella anisum L. had a lethal toxic effect on adults of B. tabaci , while the oils of P. anisum, T. vulgaris, and O. gratissimum showed repellent action . Based on their repellent effect and high toxicity on adults, the essential oils of O. gratissimum and T. vulgaris could be candidates to be developed as pesticides intended for management of B. tabaci Biotype B.
The resistance of microorganisms to conventional antimicrobials is one of the most serious health... more The resistance of microorganisms to conventional antimicrobials is one of the most serious health problems that affect not only the human, but also animals and plants, making the search for antimicrobial active ingredients a priority of global research. Green synthesis of antimicrobial silver nanoparticles (AgNPs) is a simple, cost-effective, rapid, reproducible, and environment friendly alternative for which numerous plant species have been reported for this purpose. Previous studies have shown the potential of Leea coccinea leaves in to the biosynthesis of antimicrobial silver nanoparticles. The current research aimed to study the kinetics of the reaction of synthesis of AgNPs by cationic bio-reduction from this botanical bioresource. A technology for the synthesis of AgNPs was established and the influence of operational parameters such as the bio-reduction conditions and the kinetics of the reaction were studied. AgNPs were characterized at different times by UV-VIS spectroscopi...
The essential oils are a powerful natural resource with antibacterial activity. This work aims at... more The essential oils are a powerful natural resource with antibacterial activity. This work aims at the mode of action of Lippia graveolens Kunth essential oil of plants growing in Cuba on Salmonella enterica subsp. enterica serovar Typhimurium. The effects of this oil on cell integrity were determined by time-kill, bacteriolysis and loss of 260 and 280-nm-absorbing material assays and total proteins leakage. Also, depolarization of the membrane by essential oil was monitored and intracellular and extracellular ATP was measured. The transmission electron microscopy (TEM) was used for observed morphologic change. Minimum inhibitory concentration (MIC) of L. graveolens essential oil and minimum bactericide concentration (MBC) were 0.4 and 0.8 mg/mL respectively. This essential oil showed a bactericidal action against over S . Typhimurium in a few minutes. After treatment, the cell lysis was not occurred, but little intracellular material and total proteins leakage were observed. This es...
Los aceites esenciales constituyen un grupo de productos promisorios como plaguicidas; en Nicarag... more Los aceites esenciales constituyen un grupo de productos promisorios como plaguicidas; en Nicaragua, su estudio y aplicacion es de gran interes como alternativa novedosa en el marco una agricultura dependiente de agroquimicos. El objetivo del presente trabajo fue establecer las potencialidades del aceite esencial de Pimienta dioica (L) Merr como candidato para el manejo de picudo negro (Cosmopolites sordidus Germ) y picudo rayado (Metamasius hemipterus Oliv), dos plagas insectiles con altos niveles de infestacion en plantaciones de platano (Musa paradisiaca L.) en la provincia Rivas, Nicaragua. La toxicidad aguda se evaluo por aplicacion topica del aceite de P. dioica sobre picudos adultos en condiciones de laboratorio.Para ambos insectos, el aceite provoco una mortalidad del 100% a una dosis de 1 µl transcurridas 24h. El aceite esencial de P. dioica posee potencialidades para el desarrollo de un producto destinado al manejo de picudo en la etapa de vivero, o en campo, en el cultivo...
Revista Cubana de Farmacia, 2019
Introduccion : La sintesis de nanoparticulas (NP) a partir de plantas constituye un area de gran ... more Introduccion : La sintesis de nanoparticulas (NP) a partir de plantas constituye un area de gran interes y crecientes avances, que devela el potencial de nuevas nanoestructuras para diferentes fines en el campo biomedico (terapeutico y diagnostico, entre otros). Objetivo : Revisar el estado del conocimiento sobre la sintesis y caracterizacion de diferentes tipos de nanoparticulas obtenidas a partir de plantas, y el potencial de las nanoparticulas metalicas como agentes antimicrobianos para fines biomedicos. Problemas de salud en los que se enfoca la revision: Se realizo una busqueda especializada en bases de datos de la Web de la Ciencia y Scopus y en revistas referenciadas, para el estudio de mas de cien articulos originales y revisiones en la tematica. Se abordo el estado del conocimiento sobre los principales problemas de salud tratados con nanoparticulas, la clasificacion de las nanoestructuras atendiendo a la dimension espacial, morfologia, composicion, estructura, origen, asi ...
The aim of this work was to determine the acaricidal activity of essential oils from plant specie... more The aim of this work was to determine the acaricidal activity of essential oils from plant species belonging to the families Myrtaceae, Lamiaceae, and Rutaceae against Tetranychus tumidus Banks. The studied essential oils are marketed in Nicaragua and obtained from eucalyptus ( Eucalyptus globulus Labill), Rosemary ( Rosmarinus officinalis L.), peppermint (Mentha piperita L.), lemon ( Citrus limon (L.) Osbeck), sweet orange ( Citrus sinensis (L.) Osbeck), and pepper ( Pimienta dioica L. Merr). The acaricidal activity of these oils and Dicofol at 1% was determined by the microimmersion method. All the evaluated essential oils caused mortalities above 50 % in the treated mites. The essential oils of P. dioica , M. piperita and C. lemon showed the highest acaricidal activity; mortality was over 90 % after 72 hours only by P. dioica essential oil.
Efecto de extractos acuosos de Helianthus annuus Lin. sobre el crecimiento de Solanum lycopersicu... more Efecto de extractos acuosos de Helianthus annuus Lin. sobre el crecimiento de Solanum lycopersicum Lin.
La familia Euphorbiaceae, con mas de 7500 especies en unos 300 generos, es una de las mas grandes... more La familia Euphorbiaceae, con mas de 7500 especies en unos 300 generos, es una de las mas grandes y diversas de las angiospermas. Las Euforbiaceas se han utilizado ampliamente en la medicina natural y la tradicional; tambien se han estudiado como fuente de sustancias de interes agrobiologico. El trabajo que se presenta aborda el estudio fitoquimico y de bioactividad de 4 especies de Euforbiaceas presentes en el macizo montanoso Nipe-Sagua-Baracoa. Se obtuvieron los extractos hidroalcoholicos de las cuatro especies a estudiar. Se determino su actividad frente a Artemia salina y se calcularon las LC50. Posteriormente, se evaluo la actividad de los extractos frente a nematodos fitoparasitos del genero Meloidogyne. Se determinaron los principales grupos de metabolitos secundarios presentes en las plantas estudiadas. Los extractos hidroalcoholicos de las cuatro especies evaluadas presentaron actividad significativa sobre A. salina, lo que indica sus potencialidades para la obtencion de s...
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Papers by Oriela Pino Pérez