Papers by OLIVIA VALENZUELA
Porcine rubulavirus is responsible for blue eye disease in porcine, which is characterized by neu... more Porcine rubulavirus is responsible for blue eye disease in porcine, which is characterized by neurological signs, corneal opacity and high mortality in young pigs. Reproductive disorders such as epididymitis and testicular atrophy are also common in sexually mature pigs. Since its identification at the beginning of 1980´s, many researches have identified the main molecular, pathological, biological and immunological characteristics of the disease. This review discusses the recent advances related to the humoral and cellular immune response to porcine rubulavirus. The importance of the Hemagglutinin-Neuraminidase (HN) protein as an inducer of specific antibodies during infection and the importance of CD4+CD8-lymphocytes during early immune responses and CD4+CD8+ lymphocytes in memory response are also discussed.
Immunological Investigations, 2010
The cellular immune response plays a critical role in the containment of persistent Mycobacterium... more The cellular immune response plays a critical role in the containment of persistent Mycobacterium tuberculosis infection; however, the immunological mechanisms that lead to its control are not completely identified. The goal of this study was to evaluate B (CD19+) and T (CD3+) peripheral blood lymphocyte profiles and T-cell subsets (CD4+ and CD8+) in patients with pulmonary tuberculosis (TB). Percentages (p = 0.02) and absolute numbers (p = 0.005) of B cells were significantly lower in patients with pulmonary TB than in healthy donors. In contrast, percentages (p = 0.12) and absolute numbers (p = 0.14) of T cells were similar in TB patients and healthy donors. No significant differences in percentages of CD4+ (p = 0.19) or CD8+ (p = 0.85) T cells between patients and healthy donors were observed. In summary, patients with pulmonary tuberculosis had a lower number of peripheral blood B lymphocytes than healthy controls.
British Journal of Nutrition, 2008
Micronutrient deficiencies are frequently associated with tuberculosis (TB) worldwide. We tested ... more Micronutrient deficiencies are frequently associated with tuberculosis (TB) worldwide. We tested the effect of exogenous vitamin E on proliferation and cytokine production of peripheral blood mononuclear cells (PBMC) from TB patients and healthy purified protein derivative (PPD) þ volunteers. Proliferation was stimulated with mycobacterial antigen (PPD) and evaluated by the incorporation of tritiated thymidine in PBMC cultured with or without 50 mM-vitamin E for 6 d. Cytokine production (IL-2 and interferon (IFN)-g) was determined by intracellular cytokine staining and by ELISA in the supernatant of PBMC stimulated for 24 h with phytohaemagglutinin or PPD. Our results show that culture with vitamin E increased (P#0·05) the antigen-induced proliferation of PBMC in TB patients but not in healthy PPD þ volunteers. No significant changes in the number of cytokine-producing cells or in the production of IFN-g were observed with vitamin E treatment. These results indicate that vitamin E may enhance the antigen-specific in vitro response of PBMC from TB patients. Tuberculosis: Vitamin E: Purified protein derivative * Corresponding author: Dr Jesús Hernández, fax þ52 662 280 0094, email [email protected] Abbreviations: IFN, interferon; PBMC, peripheral blood mononuclear cell; PPD, purified protein derivative; PHA, phytohaemagglutinin; TB, tuberculosis.
Parasitology Research, 2001
In the present work, it was demonstrated that in amoebic dysentery and amoebic liver abscess pati... more In the present work, it was demonstrated that in amoebic dysentery and amoebic liver abscess patients, the secretory response is long-lasting (>12 months); and 50% of amoebic dysentery patients developed circulating antiamoebic IgG in comparison with 100% of amoebic liver abscess individuals. A total of 83% of these individuals developed high levels of serum anti-Entamoeba histolytica IgA. However, only 10.4% of the dysentery patients showed this anti-E. histolytica antibody isotype in serum. There was no correlation between secretory and serum antiamoebic response, suggesting independent inductive and effector sites in both compartments.
Annals of Tropical Medicine and Parasitology, 2009
In a 12-month longitudinal study, a cohort of Mexican HIV+/AIDS patients was checked several time... more In a 12-month longitudinal study, a cohort of Mexican HIV+/AIDS patients was checked several times for Entamoeba infection, with the parasites identified, as E. histolytica or E. dispar, using PCR. The polymorphic region of the parasites' chitinase genes was investigated by PCR, with the variation in amplicon sizes being used as a measure of the genetic variation among the isolates. The patients found infected with Entamoeba at the start of the study displayed varied patterns of infection clearance and re-infection. The analysis of the polymorphisms in the chitinase gene revealed seven polymorphic patterns in the E. histolytica isolates investigated and three in the E. dispar isolates. Many of the patients were each re-infected with Entamoeba at least once during the 12 months of follow-up. As seen in a previous study in Mexico, none of the E. histolytica-infected patients developed any clinical symptoms of invasive amoebiasis during the follow-up period. The results highlight the complexity of the host-parasite relationship in human amoebiasis.
Molecular and Biochemical Parasitology, 2005
The intra-species polymorphism of E. histolytica and E. dispar species in endemic area is an impo... more The intra-species polymorphism of E. histolytica and E. dispar species in endemic area is an important tool for geographic distribution and spread mechanism studies. Since E. dispar and E. histolytica shears ecological niche, cell cycle, and transmission mechanism for human host, we studied the intra-specie variation and distribution of E. dispar strains obtained from cyst passers, in two neighbor rural communities in Morelos Mexico. We analyzed the polymorphic region of the quitinase protein gene in isolates of E. dispar. In 45 isolates from one community we identified 12 different CHI patterns while in 15 isolates from the other community we identified 5 different patterns. However both communities share 4 patterns. This finding suggests the presence of strains with different geographic mobility.
International Journal of Environmental Research and Public Health, 2010
For over 30 years it has been established that the Entamoeba histolytica protozoan included two b... more For over 30 years it has been established that the Entamoeba histolytica protozoan included two biologically and genetically different species, one with a pathogenic phenotype called E. histolytica and the other with a non-pathogenic phenotype called Entamoeba dispar. Both of these amoebae species can infect humans. E. histolytica has been considered as a potential pathogen that can cause serious damage to the large intestine (colitis, dysentery) and other extraintestinal organs, mainly the liver (amebic liver abscess),
Parasitology Research, 1999
The present work aimed at studying the possible association of HLA antigens with Entamoeba histol... more The present work aimed at studying the possible association of HLA antigens with Entamoeba histolytica/E. dispar asymptomatic infection in a Mexican mestizo population. A case-control design was selected for evaluation of the role of genetic markers in parasite infection. For this purpose the HLA-A, HLA-B, and HLA-DR profiles of a population of asymptomatic E. histolytica/E. dispar adult cyst passers (cases) and a corresponding nonparasitized adult group (controls) followed for 12 months were identified. Entamoeba species were identified through zymodeme patterns and/or amplification of species-specific DNA sequences. A healthy, nonparasitized group of individuals was included as a control. Our results show that apparently, no specific HLA marker is associated with the asymptomatic cyst passers' condition. These findings have to be added to previous results in which, in contrast to a demonstrated association between HLA-DR3 and amebic liver abscess in Mexican mestizo adults and infants, no significant association with amebic rectocolitis was found.
Annals of Tropical Medicine and Parasitology, 2007
... or ELISA; Patterson et al., 1990; Valenzuela et al., 2001), and response to systemic treatmen... more ... or ELISA; Patterson et al., 1990; Valenzuela et al., 2001), and response to systemic treatment with metronidazole within 96 h of the first dose. The yearly and total incidences of ALA (for the four study hospitals com-bined) were calculated, as the numbers of cases/1000 patients ...
Molecular and Biochemical Parasitology, 2005
Molecular and Biochemical Parasitology, 2005
The present work deals with the identification of a patient with two liver abscesses containing t... more The present work deals with the identification of a patient with two liver abscesses containing two different strains of Entamoeba histolytica , as defined by chitinase gene plymorphisms.
TABLA DE CONTENIDOS Investigación del MERCADO DE LA MIEL EN GUATEMALA I INTRODUCCIÓN II. OB... more TABLA DE CONTENIDOS Investigación del MERCADO DE LA MIEL EN GUATEMALA I INTRODUCCIÓN II. OBJETIVOS DEL ESTUDIO o Objetivos específicos III. METODOLOGÍA o Diseño de la investigación o Sujetos de estudio IV. CONTEXTO o El marco general del país o Aspectos políticos y económicos o Aspectos sociales y demográficos o El marco del sector de la miel V. LOS RESULTADOS DEL ESTUDIO o Producción nacional de miel
Uploads
Papers by OLIVIA VALENZUELA