Papers by Muktiningsih Nurjayadi
Typhoid fever is one of the most public health problem in the world, with approximately 17 millio... more Typhoid fever is one of the most public health problem in the world, with approximately 17 million cases of typhoid fever worldwide and 600,000 death cases each year. One method to prevent typhoid fever is vaccination. Nowadays, frequently, vaccine for typhoid fever is Ty 21a and vi polysaccharide vaccines. In other hands, it has side effect of nausea, fever, and headache. So it is still necessary to search other typhoid vaccine with minimum side effects. The purpose of this study was to obtain immunogenicity test information of recombinant protein Fim-C S. typhimurium and adjuvant alumina in Balb / C mice. The used doses were 20 μg, 40 μg, and 60 μg. Plasma was obtain then analyzed by ELISA, western immunobloting, and dot blot. Immune response of Balb / C mice from KS 1 showed significant differences antibody when dose injection 40μg / 20g BB and KS2 showed significant differences antibody when dose injection 20μg / 20g BB compared with KN. Adjuvant alumina was added to the Fi...
Prosiding Program Kreativitas Mahasiswa, 2019
Journal of Physics: Conference Series, 2021
Typhoid fever caused by Salmonella typhi is an endemic disease in Asia, including Indonesia. Base... more Typhoid fever caused by Salmonella typhi is an endemic disease in Asia, including Indonesia. Based on data from the World Health Organization (WHO), there are 81% per 100,000 people in Indonesia infected with typhoid. Based on this, development of typhoid detection kit that is simple, fast, specific and sensitive is still needed. One of the developments of detection kits currently available is the antigen and antibody interaction approach. This study aims to obtain comprehensive information about purification of recombinant Fim-C-S. typhi proteins as antigens for raw materials for manufacturing typhoid detection kits. This study uses the IMAC method with Ni2+ metal, which has advantages in high selectivity and purity. The results of this study report that purification of recombinant Fim-C-S. typhi protein inclusion bodies with variations in the binding and washing process at 3, 5, and 7 times gave percent yields correspondingly of 37.84, 39.44, and 38.21% pure protein. Previous stud...
Journal of Physics: Conference Series, 2019
Salmonella typhimurium and Escherichia coli are the bacteria the most common cause of food-borne ... more Salmonella typhimurium and Escherichia coli are the bacteria the most common cause of food-borne illness. The aim of the study is to detection S. typhimurium and E. coli by Real Time PCR from milk samples. Gene targeted of S. typhimurium is pef and the E. coli is fimC. Confirmation test of all primers in contaminated milk with S. typhimurium and E. coli gave different cycle threshold (Ct) value in range 12-13. The detection limit for S. typhimurium with primer pef was in 7, 92 pg/μL and detection limit for E. coli with primer fimC in 7, 12 pg/μL. Each primer produces distinctive melting peak. The melting peak curve S. typhimurium with pef primer is 84,17°C and the melting peak curve E. coli with fimC primer is 79,97°C Based on the result of this study; all the primers were successfully applied in detecting S. typhimurium and E. coli bacteria in the milk sample quickly, sensitive and specific as well as can determine the number of bacteria accurately.
Journal of Physics: Conference Series, 2019
Kurzichalcolactone A (1) and B (2), two chalcone derivatives were isolated from two species of In... more Kurzichalcolactone A (1) and B (2), two chalcone derivatives were isolated from two species of Indonesian Cryptocarya: Cryptocarya phoebeopsis and Cryptocarya morotaiense. The structures of the isolated compounds were elucidated based on the analysis of spectroscopic data, including UV, IR, NMR, and mass spectra. On biological evaluation of these compounds for their cytotoxic activity, it was found that (1) exhibited moderate inhibition activities against murine leukemia P388 cells while (2) was inactive.
Journal of Physics: Conference Series, 2019
Amylase (E.C.3.2.1.1) are the enzyme that works as catalyst in the hydrolysis of starch into simp... more Amylase (E.C.3.2.1.1) are the enzyme that works as catalyst in the hydrolysis of starch into simple monomers. Amylase enzymes are widely used in various industrial fields such as textile, food, paper and other industries. Compared to other organisms, yeasts can produce enzymes more effectively and safer for the environment. Amylolytic yeast can be isolated from flower substrates as it contains sugar for the very limited condition of yeast growth. This study aims to isolate, select and characterize amylolytic yeasts on the substrate of Paphiopedilum sp. from Bedugul Botanical Garden, Bali. Yeast isolation was carried out with direct and washing method, followed by screening conducted on YPSA medium with diffusion agar method. Results showed that 19 yeast isolates were obtained with characteristics of 73.9% white-mucoid, 21.05% cream-mucoid and 5.26% light flesh-mucoid. Screening results showed that 10 isolates which coded by P1, P4, P6, P10, P11, P12, P13, P14, P15 and P19, were able...
JRPK: Jurnal Riset Pendidikan Kimia, 2011
Penelitian ini bertujuan untuk meningkatkan efektivitas pembelajaran Ksp dan koloid siswa SMA Yup... more Penelitian ini bertujuan untuk meningkatkan efektivitas pembelajaran Ksp dan koloid siswa SMA Yuppentek 1 Tangerang.melalui implementasi Lesson Study. Sampel yang digunakan adalah satu kelas XI IPA dari enam kelas XI IPA yang ada di sekolah tersebut. Penelitiian dilakukan sebanyak lima siklus, dan setiap siklus terdiri dari tiga tahapan, yaitu tahap perencanaan (plan), tahap pelaksanaan (do), dan tahap refleksi (see) untuk memberikan tanggapan dan gambaran tentang hasil pelaksanaan. Indikator ketercapaian yang digunakan untuk mengetahui efektivitas pembelajaran pada penelitian ini adalah aktivitas siswa, interaksi siswa, kepuasan siswa, keterlaksanaan pembelajaran oleh siswa, dan keterlaksanaan pembelajaran oleh guru. Tiap pertemuan siswa diberikan angket untuk mengetahui tanggapan siswa mengenai pembelajaran yang dilakukan. Hasil angket siswa dan hasil pengamatan observer dianalisis lebih lanjut sehingga diperoleh data yang akurat tentang ketercapaian indikator peneltian yang disyaratkan. Hasil penelitian menunjukkan adanya peningkatan ketercapaian indikator efektivitas pembelajaran dari siklus pertama ke siklus kedua, dan seterusnya. Berdasarkan hasil yang diperoleh dapat disimpulkan bahwa Lesson study dapat meningkatkan efektivitas pembelajaran Ksp dan koloid siswa di SMA Yuppentek 1 Tangerang. Kata kunci : Efektivitas pembelajaran, Lesson Study, Ksp dan Koloid.
JRPK: Jurnal Riset Pendidikan Kimia, 2011
Penelitian ini bertujuan untuk meningkatkan kualitas pembelajaran kimia melalui Lesson Study pada... more Penelitian ini bertujuan untuk meningkatkan kualitas pembelajaran kimia melalui Lesson Study pada materi Hidrolisis Garam, Kelarutan dan Hasil Kali Kelarutan (Ksp) di SMA Negeri 7 Tangerang. Metode penelitian yang digunakan dalam penelitian ini adalah Metode deskriftif kualitatif dengan pendekatan Active Learning. Penelitian dilakukan melalui enam kali pertemuan dengan empat siklus, dan setiap siklus terdiri dari tiga tahapan, yaitu tahap perencanaan (plan), tahap pelaksanaan (do), dan tahap refleksi (see). Selanjutnya pada tiap akhir materi pokok dilakukan tes hasil belajar.Kualitas pembelajaran pada penelitian ini diukur melalui empat indikator, yaitu interaksi siswa, motivasi belajar siswa, keaktifan siswa, dan keterlaksanaan proses pembelajaran oleh guru. Hasil analisis menunjukkan bahwa keempat indikator kualitas pembelajaran mengalami perubahan ke arah positif dari empat open lesson yang dilaksanakan. Berdasarkan hasil yang diperoleh dapat disimpulkan bahwa Lesson studydengan pendekatan active learning dapat meningkatkan kualitas pembelajaran kimia pada Materi Hidrolisis Garam, Kelarutan dan Hasil Kali Kelarutan (Ksp) di SMA Negeri 7 Tangerang. Kata kunci Kualitaspembelajaran, Active Learning, Lesson Study.
JRPK: Jurnal Riset Pendidikan Kimia, 2013
The purpose of this research was to increase learning effectiveness of buffer solution through SA... more The purpose of this research was to increase learning effectiveness of buffer solution through SAVI (Somatic, Auditory, Visual, and Intellectual) approach using experiment guide and CD in class XI SMAN 21 Jakarta. It started from December 2011 until April 2012. The method was classroom action research in two rounds. This research started with preliminary analysis by teacher interview and student questionnaire to make the lesson plan. The results showed that the learning effectiveness had enhancement from first cycle to second cycle. It could be seen from the average of learning outcomes that increased from 73,89 in first round to 81,28 in second round. To increase learning effectiveness of buffer solution through SAVI approach, first we could know the dominant learning style of student in that class and the characteristic of subject that learnt. After that, teacher could determine learning method and media that suited with them. Application of experiment guide and CD as implememtati...
JRPK: Jurnal Riset Pendidikan Kimia, 2012
Matakuliah Biokimia I merupakan matakuliah wajib yang harus diikuti oleh mahasiswa Jurusan Kimia.... more Matakuliah Biokimia I merupakan matakuliah wajib yang harus diikuti oleh mahasiswa Jurusan Kimia. Mulai semester 093, matakuliah ini disampaikan secara bilingual. Tujuan penelitian ini adalah meningkatkan aktivitas mahasiswa pada pembelajaran Bilingual Biokimia I dengan model pembelajaran Cooperative Learning Tipe Student Team Achievement Division (STAD). Metode penelitian yang digunakan adalah metode Penelitian Tindakan Kelas. Penelitian dilakukan melalui tiga siklus dengan setiap siklus memiliki tahapan identifikasi dan analisis masalah, penetapan fokus permasalahan, perencanaan tindakan, pelaksanaan tindakan, observasi, evaluasi, refleksi dan rencana tindak lanjut. Hasil analisis data menunjukkan bahwa indikator interaksi yang terdiri atas 3 subindikator dari siklus I-III mengalami kenaikan. Subindikator Interaksi antar mahasiswa dari 83-86%. Subindikator Interaksi mahasiswa dengan dosen dari 86-87%, dan Subindikator Interaksi mahasiswa dengan sumber belajar dari 83-84%. Pada indikator kedua yaitu keaktifan mahasiswa yang terdiri atas 5 subindikator secara umum juga mengalami peningkatan. Subindikator kehadiran mahasiswa dalam proses pembelajaran dari 97-100%. Subindikator intensitas mahasiswa dalam penggunaan bahasa Inggris dari 72-76%. Subindikator kemampuan mahasiswa dalam bertanya dan menjawab dengan bahasa Inggris 52-68%. Subindikator kemampuan mahasiswa dalam mengungkapkan pendapat dengan bahasa Inggris 71-73% dan Subindikator kemampuan mahasiswa dalam memahami konsep Biokimia dalam bahasa Inggris sebesar 58-79%. Berdasarkan hasil yang diperoleh dapat disimpulkan bahwa proses pembelajaran biokimia I dengan model pembelajaran STAD yang disampaikan secara bilingual berhasil meningkatkan aktivitas mahasiswa dalam proses pembelajaran. Penelitian ini diharapkan menjadi landasan pengembangan proses pembelajaran bilingual biokimia lainnya terutama dalam peningkatan kualitas proses tanya-jawab dan penguasaan konsep biokimia dalam bahasa Inggris.
Salmonella typhi is bacteria causes typhoid disease in human. The mortality rate of typhoid disea... more Salmonella typhi is bacteria causes typhoid disease in human. The mortality rate of typhoid disease in Indonesia is increasing. The most common detection method currently used is serological test. However, this method is often resulted in less accurate, less sensitive and specific detection. The previous research has successfully discovered fim-C-S. typhi gene which is able to code protein to contribute in adherent or colonization in human epithelial cell. The information of fim-C-S. typhi gene is used to develop more specific and more accurate detection method for S. typhi bacteria by amplification, characterization, specificity and sensitivity assay, and continue with clinical assay. The PCR result was the amplified 0.2 kilo base (kb) DNA fragment using the fimbriae-C-S. typhi primer pairs. The specificity assay was conducted by comparing the amplicon in size of 0.2 kb S. typhi with Gram negative bacteria genome with similar characteristics. The results indicated the fimbriae-C-S. typhi primers can differentiate amplicon in size of 0.2 kb S. typhi against Enterobacter aerogenes, Klebsiella pneumonia, Vibrio cholera, Escherichia coli, and Pseudomonas aeruginosa. However, they cannot differentiate S. typhimurium which have higher homology in their genome. Sensitivity assay was conducted by determining the detection level of fim-C-S. typhi primers to S. typhi genome. The sensitivity assay showed the detected fimbriae-C primers S. typhi with 1.295 x 10-39 µg/mL DNA chromosome. The clinical assay was evaluated by comparing the PCR product of suspected patient blood, the isolated bacteria S. typhi genome served as positive control, and uninfected human blood as negative control. The PCR product from 10 suspected patient bloods showed the band size 0.2 kb equals to the size of band produced by the PCR product from isolated S. typhi bacteria genome. Meanwhile, the band was not detected in the uninfected blood. Band size 0.2 kb from the suspected patient blood sample indicated positive infection of S. typhi bacteria. The above results demonstrated the developed clinical detection for typhoid disease using fimbriae-C-S. typhi primer pairs.
JRSKT - Jurnal Riset Sains dan Kimia Terapan, 2011
Salmonella typhi is bacteria that cause typhoid disease in humans. In Indonesia, the morbidity nu... more Salmonella typhi is bacteria that cause typhoid disease in humans. In Indonesia, the morbidity number of typhoid disease tends to be increase. Thus, it has been requiring the alternative for handling or preventing that disease. Recently, the detection method commonly uses for S. typhi detection is Serological test. The weakness of this method is often producing less accurate and not specific detection. The previous research was successfully discovered S. typhi gene that codes protein which is contributed at adherents or colonization those bacteria in epithelial human cell. That result was base to develop detection on S. typhi method by Polymerase chain reaction (PCR). The aim of this research is developing a specific and accurate detection method for S. typhi bacteria by PCR. The research result is performed successfully to amplify the fimbrial-C S. typhi gene using pairs of primer FW-INT 2- REV-1A NEW which was designed and synthesized in previous step. That success showed by the f...
The concept of 21st-century education in the era of the industrial revolution 4.0 demands optimal... more The concept of 21st-century education in the era of the industrial revolution 4.0 demands optimal digitalization and computerization innovation. The utilization of technology makes the learning process take place continuously without space and time limits. This study aims to produce electronic-modules (e-modules) as instructional materials for protein structure and function using the Flip PDF Professional application. The method that used refers to the research and development of the Borg and Gall model with a questionnaire research instrument. The research and the development procedures are preliminary study, research planning, initial product development, small scale field test, revision of small scale field test results, large scale field test, and revision of large field test results The electronic module consists of an introduction and discussion of the material (based on the learning design of the Biomolecular Protein Structure and Function course in Chemistry Education underg...
21st-century environmental education requires integration in learning natural science. That is du... more 21st-century environmental education requires integration in learning natural science. That is due to strengthen students' understanding of the environment. This study aimed to develop an electronic module for enrichment programs in the form of science, environment, technology, and society (SETS) called EM-SETS. The research method used a research and development model. The instrument used in this study was a questionnaire. The module developed was validated by 3 learning topics and language experts and 3 media experts. The module was tested by 94 Class X students at Vocational School and 8 chemistry subject teachers. The results of the EM-SETS assessment by experts, teachers, and students are classified as high to very high, so it can be concluded that the EM-SETS that was developed was feasible to be used as a learning medium for students at Vocational School.
Detection of food-borne bacterial pathogens was developed to overcome the limitations. The aim of... more Detection of food-borne bacterial pathogens was developed to overcome the limitations. The aim of this research was to develop Salmonella typhimurium detection by Real Time Polymerase Chain Reaction (RT-PCR) using two pairs of primers. The ability of primer pairs to detect S. typhimurium is seen from cycle threshold or Ct. Artificially contaminated milk sample with 24 ng each microliter can be detected with fljB (flagellin gene) primers on Ct 12,933 and with STM4497 (hypothetical protein code) primers on Ct 13,665. The specificity test of both primers showed that melting temperature (Tm) of fljB was 80,5 degree Celsius, and STM449 was 81,6 degree Celsius. FljB and STM4497 primers gave an average detection limit respectively of 11,75 Colony Forming Unit (CFU) each milliliter and 6,8 CFU each milliliter. The time needed throughout the detection process of S. typhimurium with fljB and STM4497 primers is faster than conventional methods. Based on the results it can be concluded that pri...
Catharanthus roseus L. (Apocynacea) which is also known as Madagascar periwinkle and Vinca rosea ... more Catharanthus roseus L. (Apocynacea) which is also known as Madagascar periwinkle and Vinca rosea is historically been used for the treatment of various diseases. The assessment of this plant extracts to be used as prophylactic agent against certain human pathogens has been thoroughly investigated by previous researchers. It is an important plant for novel pharmaceuticals since most of the pathogens are developing resistance against currently available antimicrobial drugs. Thus, this review is summarizing the active compounds which are responsible for bioactivity of the plant and provides insight into genetic regulation of alkaloids containing in the extracts to serve as antimicrobial agents. Further research on Catharanthus roseus is highly recommended to explore its full potential as phytomedicine specifically to be developed as antimicrobial drug.
The COVID-19 pandemic occurred in many parts of the world has disrupted many aspects of life. One... more The COVID-19 pandemic occurred in many parts of the world has disrupted many aspects of life. One precaution to protect ourselves from this virus is cleaning our hands by using hand sanitizers and cleaning surfaces that are frequntly touched by using disinfectants. Therefore, there is tremendous need of this chemical substance in this pandemic era. This fact enforces people to make this chemical at home.. This homemade manufacture and use of sanitizers and disinfectants must be in accordance with the regulations, so that they don't cause any harmful effects for the body. This program is carried out with the aim of providing education to the community, especially residents of Duren Sawit Village. The media used in this program are Instagram and YouTube, and the materials were delivered in form of videos. Videos were uploaded to Instagram and YouTube on 2 – 23 June 2020. After that, a questionnaire was filled on 23 June – 1 July 2020. From the questionnaire data, it can be shown t...
Salmonella typhi is bacteria that cause typhoid fever for human. In Indonesia, the morbidity rate... more Salmonella typhi is bacteria that cause typhoid fever for human. In Indonesia, the morbidity rate of typhoid fever’s suffer tends to increase. The common method to prevent typhoid fever is to weaken the vaccine of S. typhi bacteria. However this kind of vaccine has weaknesses that occur in several side effects, such as nausea, vomit, fever, and headache. Therefore this research is aimed to isolate and to characterize the gene structure of fim-C S. Typhi local strain of 0.8 kilobase as an initial study to develop the recombinant vaccine. This research used explorative method that consists of (1) designing the specific primer of fim-C S. typhi gene; (2) synthesize the specific primer pair of fim-C S. typhi gene; (3) isolate the genome of S. typhi bacteria; (4) amplificate the fim-C gene by PCR; (5) characterize the amplification result fim-C S. typhi gene. The specific primer from the designing process (Fw 1b-Rev 1a New) has succeeded to amplify the S. typhi genome showed by one band ...
The Fim-C-S typhi recombinant protein is a protein developed from the surface of the Salmonella t... more The Fim-C-S typhi recombinant protein is a protein developed from the surface of the Salmonella typhi bacteria. This protein has been known to cause a good immune response. The protein has the potential as a raw material for immuno chromatography-based with gold nano particles as rapid detection kits. Various alternatives are taken to obtain pure protein with the maximum amount. Previous studies have succeeded in purifying the Fim-C-S. typhi recombinant protein with the Ni/NTA approach. This study aims to obtain information on optimum conditions for Fim-C-S. typhi recombinant protein purification with Co/NTA resin as raw material for rapid detection kit, because Co/NTA resin is also known to have good specifications for purification of recombinant proteins with Histidine tagging. There are three main stages in the purification process in this system, namely binding, washing, and elution. The binding and washing processes in this study were carried out 2, 4 and 6 times. Furthermore, ...
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Papers by Muktiningsih Nurjayadi