Papers by Nina Sanapareddy
Genetics in medicine open, 2024
Kidney international reports, Jun 1, 2024
medRxiv (Cold Spring Harbor Laboratory), Feb 14, 2024
doi: medRxiv preprint NOTE: This preprint reports new research that has not been certified by pee... more doi: medRxiv preprint NOTE: This preprint reports new research that has not been certified by peer review and should not be used to guide clinical practice.
Obstetrics & Gynecology
OBJECTIVE: To identify conditions on a reproductive carrier screening panel with the potential fo... more OBJECTIVE: To identify conditions on a reproductive carrier screening panel with the potential for carrier manifestations during pregnancy and review the implications for obstetric care. METHODS: This was a retrospective cross-sectional study of consecutive samples from female patients aged 18–55 years submitted to a commercial laboratory for a 274-gene carrier screening panel (January 2020 to September 2022). A literature review was performed to identify genes on the panel with potential for pregnancy complications in carriers. Carrier expression and published recommendations for clinical management were reviewed. RESULTS: We identified 12 genes with potential for carrier manifestations during pregnancy based on reports in the literature: nine with manifestations irrespective of the fetal genetic status (ABCB11, COL4A3, COL4A4, COL4A5, DMD, F9, F11, GLA, and OTC) and three (CPT1A, CYP19A1, and HADHA) with manifestations only if the fetus is affected by the condition. Manifestations...
Genetics in Medicine Open
Molecular Genetics & Genomic Medicine, Nov 5, 2022
Genetics in Medicine, 2022
Transcriptional profile of right ventricular tissue during
Acute pulmonary embolism (PE) is the third leading cause of cardiovascular death in the United St... more Acute pulmonary embolism (PE) is the third leading cause of cardiovascular death in the United States. Moderate to severe PE can cause pulmonary arterial hypertension (PH) with resultant right ventricular (RV) heart damage. The mechanisms leading to RV failure after PE are not well defined, although it is becoming clear that PH-induced inflammatory responses are involved. We have previously demonstrated profound neutrophil-mediated inflammation and RV dysfunction during PE which was associated with increased expression of several chemokine genes. However, a complete assessment of transcriptional changes in RVs during PE is still lacking. We have now used DNA microarrays to assess the alterations in gene expression in right ventricular tissue during acute PE/PH in rats. Key results were confirmed with Real-Time RT PCR. Nine CCchemokine genes (CCL-2, 3, 4, 6, 7, 9, 17, 20, 27), 5 CXC-chemokine genes (CXCL-1, 2, 9, 10, 16) and the receptors CCR1 and CXCR4 were up-regulated after 18 hou...
Background: Experimental models of pulmonary embolism (PE) that produce pulmonary hypertension (P... more Background: Experimental models of pulmonary embolism (PE) that produce pulmonary hypertension (PH) employ many different methods of inducing acute pulmonary occlusion. Many of these models induce PE with intravenous injection of exogenous impervious objects that may not completely reproduce the physiological properties of autologous thromboembolism. Current literature lacks a simple, well-described rat model of autlogous PE. Objective: Test if moderate-severity autologous PE in Sprague-Dawley (SD) and Copenhagen (Cop) rats can produce persistent PH. Methods: blood was withdrawn from the jugular vein, treated with thrombin-Ca ++ and re-injected following pretreatment with tranexamic acid. Hemodynamic values, clot weights and biochemical measurements were performed at 1 and 5 days. Results: Infusion of clot significantly increased the right ventricular peak systolic pressure to 45-55 mm Hg, followed by normalization within 24 hours in SD rats, and within 5 days in COP rats. Clot lysi...
Molecular Genetics and Metabolism
Molecular Genetics and Metabolism, 2021
Microbes are abundant on earth and play a crucial role in the environment they inhabit. Before th... more Microbes are abundant on earth and play a crucial role in the environment they inhabit. Before the dawn of metagenomics, the study of the effect of microorganisms on their environment was limited due to use of low throughput techniques that could only examine single organisms or a few at a time. Metagenomics is a fast growing field of science that permits investigation of microbes by directly extracting DNA from the environment. A lot of environments, ranging in complexity from the ocean to acid mines, from wastewater communities to the human body have been targeted by metagenomics studies, and these studies generate tremendous amounts of data and newer and more efficient bioinformatic tools and methods are needed to interpret this complex data. In this dissertation we used bioinformatic tools to enrich our understanding of the role that microorganisms play within some important but understudied microbial environments. In Chapter 1, we report an increased microbial richness associat...
Thrombosis journal, Jan 11, 2010
Experimental models of pulmonary embolism (PE) that produce pulmonary hypertension (PH) employ ma... more Experimental models of pulmonary embolism (PE) that produce pulmonary hypertension (PH) employ many different methods of inducing acute pulmonary occlusion. Many of these models induce PE with intravenous injection of exogenous impervious objects that may not completely reproduce the physiological properties of autologous thromboembolism. Current literature lacks a simple, well-described rat model of autlogous PE. Test if moderate-severity autologous PE in Sprague-Dawley (SD) and Copenhagen (Cop) rats can produce persistent PH. blood was withdrawn from the jugular vein, treated with thrombin-Ca++ and re-injected following pretreatment with tranexamic acid. Hemodynamic values, clot weights and biochemical measurements were performed at 1 and 5 days. Infusion of clot significantly increased the right ventricular peak systolic pressure to 45-55 mm Hg, followed by normalization within 24 hours in SD rats, and within 5 days in COP rats. Clot lysis was 95% (24 hours) and 97% (5 days) in S...
The Journal of Eukaryotic Microbiology, 2003
Transgenic and knockout mice usefully model the mechanisms that result in the clearance of Crypto... more Transgenic and knockout mice usefully model the mechanisms that result in the clearance of Cryptosporidium parvum from the gut. CD4+ cells, cells expressing MHC class II, and CD154/CD40 interactions are essential. Unexpectedly, AND RAG-/- and DO11.10 RAG-/- mice with single specificities of T cells successfully clear Cryptosporidium infection. Clearance is accompanied by activation of CD4+ cells in the MLN. The ability of T cells bearing receptors for apparently irrelevant and non-cross reactive antigens to activate and to clear infection is surprising. The requirement for class II MHC expression for Cryptosporidium clearance raises the alternative possibilities that (a) class II MHC is required to present a peptide that is loaded as a consequence of infection or (b) that the cytokine environment engendered by a Cryptosporidium infection allows affinity for self MHC to activate naive T cells. In order to test the hypothesis that peptide loading is necessary, we used A betaE alpha-/-Ii-/- mice that express a hybrid IA-IE MHC molecule. They also carry a transgene that makes an E alpha peptide while disruption of their invariant chain blocks the loading of a foreign peptide on to their MHC class II molecules. After oral gavage, the course of infection was followed by ELISA. CD4+ cells in the MLN of these mice were activated to express CD69 and the infection was cleared. We conclude that the loading of a Cryptosporidium or other infection-dependent peptide onto the MHC class II molecules of APCs is not necessary for clearance of Cryptosporidium. Instead the TcR affinity for self-MHC must suffice for T cell activation in the cytokine environment resulting from infection.
The ISME Journal, 2012
Differences in the composition of the gut microbial community have been associated with diseases ... more Differences in the composition of the gut microbial community have been associated with diseases such as obesity, Crohn's disease, ulcerative colitis and colorectal cancer (CRC). We used 454 titanium pyrosequencing of the V1-V2 region of the 16S rRNA gene to characterize adherent bacterial communities in mucosal biopsy samples from 33 subjects with adenomas and 38 subjects without adenomas (controls). Biopsy samples from subjects with adenomas had greater numbers of bacteria from 87 taxa than controls; only 5 taxa were more abundant in control samples. The magnitude of the differences in the distal gut microbiota between patients with adenomas and controls was more pronounced than that of any other clinical parameters including obesity, diet or family history of CRC. This suggests that sequence analysis of the microbiota could be used to identify patients at risk for developing adenomas.
Physiological Genomics, 2008
Acute pulmonary embolism (PE) is the third leading cause of cardiovascular death in the United St... more Acute pulmonary embolism (PE) is the third leading cause of cardiovascular death in the United States. Moderate to severe PE can cause pulmonary arterial hypertension (PH) with resultant right ventricular (RV) heart damage. The mechanisms leading to RV failure after PE are not well defined, although it is becoming clear that PH-induced inflammatory responses are involved. We previously demonstrated profound neutrophil-mediated inflammation and RV dysfunction during PE that was associated with increased expression of several chemokine genes. However, a complete assessment of transcriptional changes in RVs during PE is still lacking. We have now used DNA microarrays to assess the alterations in gene expression in RV tissue during acute PE/PH in rats. Key results were confirmed with real-time RT-PCR. Nine CC-chemokine genes (CCL-2, -3, -4, -6, -7, -9, -17, -20, -27), five CXC-chemokine genes (CXCL-1, -2, -9, -10, -16), and the receptors CCR1 and CXCR4 were upregulated after 18 h of mod...
Gastroenterology- …, 2011
The gut microbiota is closely involved in normal host physiology and may contribute to the etiolo... more The gut microbiota is closely involved in normal host physiology and may contribute to the etiology of many diseases. Colorectal cancer (CRC) is the third cause of cancer death in the United States. Evidence from animal and human studies supports a link between ...
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Papers by Nina Sanapareddy