Neylla Sena

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Jaswinder K Sethi

University of Cambridge

Anil Sukumaran

Hamad Medical Corporation

David Gortler

Yale University

Jorge Iván Castillo-Quan

Harvard Medical School

Baharuddin Baharuddin

Universitas Surabaya

CLEMENT C AZODO

University of Benin

Dustin Hittel

University of Calgary

Dr. Syed Wali Peeran

Sebha University

Mudhir Surchi

Salahaddin University-Erbil

Ghada Abdel-hamid

King AbdulAziz University (KAU) Jeddah, Saudi Arabia

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Papers by Neylla Sena

Antimicrobial activity of AH Plus and Sistema Epiphany sealers and disinfected cone of gutt-percha and Resilon

Orientador: Brenda Paula Figueiredo de Almeida GomesTese (doutorado) - Universidade Estadual de C... more Orientador: Brenda Paula Figueiredo de Almeida GomesTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de PiracicabaResumo: Este estudo investigou: a) a atividade antimicrobiana de cimentos endodônticos (AH Plus e Epiphany) frente a microrganismos encontrados na microbiota intracanal (Enterococcus faecalis, Staphylococcus aureus, Actinomyces naeslundii e Candida albicans); b) a microinfiltração coronária de dentes obturados com AH Plus/ guta-percha e Epiphany/ Resilon; c) os possíveis efeitos antimicrobianos residuais destas substâncias nos cones quando em contato com E. faecalis e Porphyromonas gingivalis, d) as alterações na superfície dos cones de guta-percha e Resilon expostos à desinfecção com clorexidina gel 2% e hipoclorito de sódio 5,25%. A ação antimicrobiana dos cimentos foi testada através de dois métodos: o de contato direto nos tempos: imediato, 24 h, 48 h, 7 e 14 dias após a espatulação, e através do teste de difusão em Agar. A avaliação da ...

Estudo in vitro da atividade antimicrobiana do hipoclorito de sodio e da clorexidina usados como substancias quimicas auxiliares frente a biofilmes de especie unica

O objetivo deste estudo foi investigar a atividade antimicrobiana do hipoclorito de sodio (NaOCl)... more O objetivo deste estudo foi investigar a atividade antimicrobiana do hipoclorito de sodio (NaOCl) 2,5% e 5,25% e da clorexidina (CLX) 2.0% tanto na forma gel como liquida utilizados como substância quimica auxiliar durante o preparo quimico-mecânico frente a biofilmes de especies unica. Biofilmes simples dos microrganismos Enterococcus faecalis, Staphylococcus aureus, Prevotella intermedia, Porphyromonas gingivalis, Porphyromonas endodontalis, Fusobacterium nucleatum e Candida albicans foram formados em filtros de membrana de nitrato de celulose sobre placas de agar sangue. Os biofilmes foram imersos nas substâncias quimicas por 30s, 5, 10, 15, 30 e 60 min com ou sem agitacao mecânica e em seguida transferidos para meios de cultura contendo neutralizadores das substâncias quimicas. A seguir, foram realizadas diluicoes em serie, aliquotas foram inoculadas em placas de agar sangue, incubadas e apos crescimento, as unidades formadoras de colonias foram quantificadas atraves de suas diluicoes. O hipoclorito de sodio (NaOCl) 5,25% eliminou todos microrganismos testados em 30 segundos de contato. Frente aos microrganismos anaerobios estritos, todas as substâncias quimicas obtiveram o mesmo desempenho, sendo efetivas em 30 segundos. A solucao salina permitiu o crescimento microbiano de todas as cepas. Concluiu-se que NaOCl a 5,25% foi a substância quimica testada mais efetiva, seguido pela CLX liquida 2%. Os resultados demonstraram que a efetividade do agente antimicrobiano depende dos microrganismos que constituem o biofilme, do tempo de contato destes com o a substância quimica, da acao ou nao da agitacao mecânica e forma de apresentacao da substância. Abstract

Efficacy of various concentrations of NaOCl and instrumentation techniques in reducing Enterococcus faecalis within root canals and dentinal tubules

International Endodontic Journal, 2006

Berber VB, Gomes BPFA, Sena NT, Vianna ME, Ferraz CCR, Zaia AA, Souza-Filho FJ. Efficacy of vario... more

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Propriedades antimicrobianas do cimento AH Plus, Sistema Epiphany e de cores de guta-percha e Resilon desinfectados

Este estudo investigou: a) a atividade antimicrobiana de cimentos endodonticos (AH Plus e Epiphan... more Este estudo investigou: a) a atividade antimicrobiana de cimentos endodonticos (AH Plus e Epiphany) frente a microrganismos encontrados na microbiota intracanal (Enterococcus faecalis, Staphylococcus aureus, Actinomyces naeslundii e Candida albicans); b) a microinfiltracao coronaria de dentes obturados com AH Plus/ guta-percha e Epiphany/ Resilon; c) os possiveis efeitos antimicrobianos residuais destas substâncias nos cones quando em contato com E. faecalis e Porphyromonas gingivalis, d) as alteracoes na superficie dos cones de guta-percha e Resilon expostos a desinfeccao com clorexidina gel 2% e hipoclorito de sodio 5,25%. A acao antimicrobiana dos cimentos foi testada atraves de dois metodos: o de contato direto nos tempos: imediato, 24 h, 48 h, 7 e 14 dias apos a espatulacao, e atraves do teste de difusao em Agar. A avaliacao da microinfiltracao coronaria de dentes obturados endodonticamente foi acompanhada diariamente por 90 dias apos a contaminacao. A acao residual dos agentes desinfetantes sobre a superficie dos cones foi observada atraves do metodo de difusao em Agar (nos periodos de 1, 5, 10, 20 e 30 min), e as alteracoes estruturais na superficie dos cones foram testadas atraves de analise em Microscopio Eletronico de Varredura (MEV). Quanto ao metodo de contato direto os resultados foram: O cimento AH Plus teve maior atividade antimicrobiana que o Epiphany, o Epiphany apresentou grande dissolucao em todos os tempos testados (p<0.05), A. naeslundii foi o microrganismo mais suscetivel e E. faecalis o mais resistente a todos os cimentos testados. Quanto ao metodo de difusao em Agar a maior atividade antimicrobiana foi a do Primer, seguida do AH Plus e do Epiphany. C. albicans foi o microrganismo mais suscetivel e o S. aureus o mais resistente. Quanto a prevencao da microinfiltracao coronaria, AH Plus/guta-percha foi superior ao sistema Epiphany (p<0.05). Os dentes obturados com o sistema Epiphany apresentaram clinicamente alteracoes de cor em suas raizes. Quanto a atividade antimicrobiana das substâncias quimicas auxiliares sobre os cones, estas foram incapazes de se unirem aos componentes dos cones de guta-percha e de apresentarem efeito residual. Entretanto o cone de Resilon apresentou acao antibacteriana residual quando exposto a clorexidina gel. Quanto as alteracoes estruturais dos cones em MEV, nao foi encontrada modificacao nas caracteristicas superficiais dos cones e nem presenca de cristais de cloreto. Em conclusao, os resultados sugerem que o sistema Epiphany nao demonstrou ser superior ao AH Plus quanto a acao antimicrobiana e a prevencao da microinfiltracao coronaria, e que as substâncias quimicas auxiliares usadas na desinfeccao dos cones de guta-percha e de Resilon nao apresentaram acao residual nos cones de guta-percha e nem alteraram a superficie dos dois tipos de cones testados. Entretanto o cone de Resilon apresentou acao antibacteriana residual quando exposto a clorexidina gel Ab

Accidental nasal fossa perforation during endodontic treatment - Case report

Research, Society and Development, 2021

Fracture of an endodontic instrument within the root canal system can occur due to incorrect use ... more Fracture of an endodontic instrument within the root canal system can occur due to incorrect use of instruments, and clinicians are confronted with a few removal options when considering this situation. The purpose of this article is to present the removal of a fractured endodontic file from the periapical region of the right upper central incisor, that caused a nasal floor perforation and otorhinolaryngological symptoms, with the aid of a dental operating microscope (DOM) and cone bean computed tomography (CBCT). Success was achieved when the fragment was visible and removed from the nasal fossa. The standardized techniques of removal or bypassing fracture file were not effective, and success was obtained with the aid of CBCT that made possible the visualization of the broken file inside the nasal fossa.

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In vitro evaluation of the antimicrobial activity of calcium hydroxide combined with chlorhexidine gel used as intracanal medicament

Introduction: When performing endodontic treatment, the dentalsurgeon must find ways to eliminate... more

Influence of different adhesive systems on bond strength of carbon fiber posts used to restore endodontically treated teeth

Journal of Research in Dentistry

The aim of this study was to evaluate the influence of three adhesive systems on resinous bonding... more The aim of this study was to evaluate the influence of three adhesive systems on resinous bonding of carbon fiber posts on roots of endodontically treated bovine incisors. Thirty bovine lower incisors with similar dimensions were selected for this study. The roots were endodontically treated and subsequently prepared for post cementation. The posts were treated with adhesive systems Scotchbond MultiPurpose Plus – 3M chemical cure (Group I), light cure (Group II) and dual cure (Group III). Carbon fiber posts were adjusted to 8mm and cemented in the canal with resinous dual cement RelyX ARC – 3M. Those were taken to Universal Essay Machine for the push-out shear bond strength test. Analysis of the results were made with 2-Way ANOVA and post-hoc with Tukey’s test (p<0.05). The groups were statistically different. The chemical cure adhesive system (Group I) showed highest values of resistance to push-out, both at all thirds as well as in total average (24.77 MPa), followed by light c...

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Conservative option of retreatment for gutta-percha overfilled root canal: a case report

Oral Sciences, Oct 12, 2013

In vitro evaluation of the antimicrobial activity of calcium hydroxide combined with chlorhexidine gel used as intracanal medicament

Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics, 2006

The aim of this study was to investigate the antimicrobial activity of calcium hydroxide (Ca(OH)2... more The aim of this study was to investigate the antimicrobial activity of calcium hydroxide (Ca(OH)2) combined with 2% chlorhexidine gluconate (CHX) gel against endodontic pathogens and to compare the results with the ones achieved by Ca(OH)2 mixed with sterile water and by CHX gel alone. Two methods were used: the agar diffusion test and the direct contact test. Ca(OH)2 + 2% CHX gel produced inhibitory zones ranging from 2.84 to 6.5 mm, and required from 30 seconds to 6 hours to eliminate all tested microorganisms. However, 2% CHX gel showed the largest microbial growth zones from 4.33 to 21.67 mm, and required 1 minute or less to inhibit all tested microorganisms. A paste of Ca(OH)2 plus sterile water inhibited only the microorganisms with which it was in direct contact and required from 30 seconds to 24 hours to kill all tested microorganisms. In conclusion, 2% CHX gel + Ca(OH)2 showed better antimicrobial activity than Ca(OH)2 manipulated with sterile water.

Residual Effects and Surface Alterations in Disinfected Gutta-Percha and Resilon Cones

Journal of Endodontics, 2007

The aim of this study was to evaluate alterations on the surface of gutta-percha and Resilon cone... more The aim of this study was to evaluate alterations on the surface of gutta-percha and Resilon cones when exposed to 2% chlorhexidine gel (CHX) and 5.25% sodium hypochlorite (NaOCl) and to evaluate their possible antibacterial residual effects against Enterococcus faecalis and Porphyromonas gingivalis. A total of 270 medium tapered cones (135 gutta-percha and 135 Resilon) were selected and divided into three groups, according to the substance (NaOCl, CHX, and sterile saline solution). The cones were immersed in each substance (for 1, 5, 10, 20, and 30 min), rinsed in saline solution, and dried. They were placed onto inoculated agar plates and incubated under specific conditions. Scanning electron microscopy analysis was performed on additional 270 disinfected cones. Another 18 cones were used as controls. Based on the results, it was concluded that Resilon cones exposed to CHX for 10, 20, and 30 min demonstrated residual antibacterial action and that substances did not cause alterations to the cones' surface. (J Endod 2007;33:948 -951)

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In vitro antimicrobial activity of sodium hypochlorite and chlorhexidine against selected single-species biofilms

International Endodontic Journal, 2006

Sena NT, Gomes BPFA, Vianna ME, Berber VB, Zaia AA, Ferraz CCR, Souza-Filho FJ. In vitro antimicr... more

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In vitro evaluation of the susceptibility of endodontic pathogens to calcium hydroxide combined with different vehicles

Brazilian Dental Journal, 2005

The aim of this study was to investigate in vitro the antimicrobial activity of calcium hydroxide... more The aim of this study was to investigate in vitro the antimicrobial activity of calcium hydroxide [Ca(OH)2] in combination with different vehicles against endodontic pathogens. For such purpose, a broth dilution test was performed. Pastes were prepared with Ca(OH)2 powder and the following vehicles: sterile water, glycerin, camphorated monochlorophenol (CMCP), CMCP + glycerin, polyethyleneglycol and CMCP + polyethyleneglycol. The time required for the pastes to produce negative cultures against the tested microorganisms was recorded and analyzed statistically using the Kruskal Wallis test at 5% significance level. Timing for pastes to eliminate the aerobic and facultative anaerobic microorganisms ranged from 6 to 24 h, while strict anaerobic microorganisms were inhibited within 30 s to 5 min. Microbial susceptibility, ranked from weakest to strongest, can be presented as follows: Enterococcus faecalis (the most resistant microorganism), Candida albicans, Staphylococcus aureus, Porphyromonas gingivalis, Porphyromonas endodontalis and Prevotella intermedia (the last two microorganisms required the same time to be eliminated). In conclusion, calcium hydroxide pastes needed more time to eliminate facultative than anaerobic microorganisms. These findings suggest that the antimicrobial property is related both to paste formulation and to microbial susceptibility.

In vitro evaluation of the antimicrobial activity of lichen metabolites as potential preservatives

by Maria Tognim and Neylla Sena

Antimicrobial Agents and Chemotherapy, 1985

Antimicrobial activity of AH Plus and Sistema Epiphany sealers and disinfected cone of gutt-percha and Resilon