Books by Mun Mun Mukherjee
Infectious diseases caused by bacterial foodborne pathogens represent a worldwide and serious pub... more Infectious diseases caused by bacterial foodborne pathogens represent a worldwide and serious public health concern due to relatively high morbidity and mortality rates. Although chemotherapies are available for foodborne infections, bacterial variants continually evolve and provide resistance to clinically relevant antimicrobial chemotherapeutic agents potentially confounding effective treatments, especially when such clinical pathogens are multidrug resistant. There are several bacterial mechanisms that confer multidrug resistance, such as enzymatically-based drug destruction, modification of key drug targets, reduced drug entry into cells of pathogens, and active drug and multidrug efflux from pathogenic bacteria. Of these drug resistance mechanisms, multidrug efflux pump systems from a very large group of closely-related proteins constituting the well-known major facilitator superfamily embody an important and major class for determining foodborne pathogen drug resistance. This book chapter briefly summaries recent developments for key multidrug efflux pumps of the major facilitator superfamily from key foodborne bacterial pathogens.
Papers by Mun Mun Mukherjee
Anti-Infective Agents, 2016
Vibrio cholerae is a Gram-negative bacterium and causative agent of cholera , which is a severe g... more Vibrio cholerae is a Gram-negative bacterium and causative agent of cholera , which is a severe gastrointestinal disease and a global public health concern. Repletion and antimicrobial drug therapies are necessary for efficacious treatment of severe cases of cholera. In situations where proper sanitation and repletion therapy are unavailable and multidrug resistant V. cholerae variants are present, alternative means of therapy are indicated. Towards this, comparative genomic analyses between virulent versus non-virulent serotypes of V. cholerae have been useful in identifying new cellular targets for the development of new chemotherapies for cholera. This review article explores these new avenues for cholera treatments and summarizes these novel putative drug targets.
Trends in cell & molecular biology
The biological membrane is an efficient barrier against water-soluble substances. Solute transpor... more The biological membrane is an efficient barrier against water-soluble substances. Solute transporters circumvent this membrane barrier by transporting water-soluble solutes across the membrane to the other sides. These transport proteins are thus required for all living organisms. Microorganisms, such as bacteria, effectively exploit solute transporters to acquire useful nutrients for growth or to expel substances that are inhibitory to their growth. Overall, there are distinct types of related solute transporters that are grouped into families or superfamilies. Of these various transporters, the major facilitator superfamily (MFS) represents a very large and constantly growing group and are driven by solute- and ion-gradients, making them passive and secondary active transporters, respectively. Members of the major facilitator superfamily transport an extreme variety of structurally different substrates such as antimicrobial agents, amino acids, sugars, intermediary metabolites, io...
Pathogenic and opportunistic bacteria which are causative agents of infectious disease and harbor... more Pathogenic and opportunistic bacteria which are causative agents of infectious disease and harbor effective drug resistance mechanisms may confound treatment in human clinical medicine. Of the various antimicrobial agent resistant mechanisms, active efflux by secondary active transporter systems, called drug efflux pumps, are commonly found within infectious disease causing bacteria. These secondary active drug efflux systems are energized by cation gradients and may have single or multiple drug substrates. It is these multidrug efflux systems that compromise chemotherapeutic efforts against multiple drug resistant bacteria. Close scrutiny of these multidrug efflux systems may provide useful molecular information for potential modulation in order to reduce the conditions that foster bacterial drug resistance and thus possibly restore clinical efficacy of antimicrobial agents. This chapter focuses on key bacterial secondary drug efflux pump systems from a molecular perspective.
International Journal of Environmental Research and Public Health, 2015
Foodborne illnesses caused by bacterial microorganisms are common worldwide and constitute a seri... more Foodborne illnesses caused by bacterial microorganisms are common worldwide and constitute a serious public health concern. In particular, microorganisms belonging to the Enterobacteriaceae and Vibrionaceae families of Gram-negative bacteria, and to the Staphylococcus genus of Gram-positive bacteria are important causative agents of food poisoning and infection in the gastrointestinal tract of humans. Recently, variants of these bacteria have developed resistance to medically important chemotherapeutic agents. Multidrug resistant Escherichia coli, Salmonella enterica, Vibrio cholerae, Enterobacter spp., and Staphylococcus aureus are becoming increasingly recalcitrant to clinical treatment in human patients. Of the various bacterial resistance mechanisms against antimicrobial agents, OPEN ACCESS Int. J. Environ. Res. Public Health 2015, 12 1488
Genomics Discovery, 2014
Pathogenic strains of Vibrio cholerae are responsible for endemic and pandemic outbreaks of the d... more Pathogenic strains of Vibrio cholerae are responsible for endemic and pandemic outbreaks of the disease cholera. The complete toxigenic mechanisms underlying virulence in Vibrio strains are poorly understood. The hypothesis of this work was that virulent versus non-virulent strains of V. cholerae harbor distinctive genomic elements that encode virulence. The purpose of this study was to elucidate genomic differences between the O1 serotypes and non-O1 V. cholerae PS15, a non-toxigenic strain, in order to identify novel genes potentially responsible for virulence. In this study, we compared the whole genome of the non-O1 PS15 strain to the whole genomes of toxigenic serotypes at the phylogenetic level, and found that the PS15 genome was distantly related to those of toxigenic V. cholerae. Thus we focused on a detailed gene comparison between PS15 and the distantly related O1 V. cholerae N16961. Based on sequence alignment we tentatively assigned chromosome numbers 1 and 2 to elements within the genome of non-O1 V. cholerae PS15. Further, we found that PS15 and O1 V. cholerae N16961 shared 98% identity and 766 genes, but of the genes present in N16961 that were missing in the non-O1 V. cholerae PS15 genome, 56 were predicted to encode not only for virulence-related genes (colonization, antimicrobial resistance, and regulation of persister cells) but also genes involved in the metabolic biosynthesis of lipids, nucleosides and sulfur compounds. Additionally, we found 113 genes unique to PS15 that were predicted to encode other properties related to virulence, disease, defense, membrane transport, and DNA metabolism. Here, we identified distinctive and novel genomic elements between O1 and non-O1 V. cholerae genomes as potential virulence factors and, thus, targets for future therapeutics. Modulation of such novel targets may eventually enhance eradication efforts of endemic and pandemic disease cholera in afflicted nations. Comparative genome analysis of non-toxigenic non-O1 versus toxigenic O1 Vibrio cholerae. Genomics Discov. 2014; 2:1. http://dx.
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Books by Mun Mun Mukherjee
Papers by Mun Mun Mukherjee