The new species Inocybe quercicola is described on the basis of morphological and genetic feature... more The new species Inocybe quercicola is described on the basis of morphological and genetic features. It is characterized by medium-sized basidiomata, radially fibrillose campanulate pileus, dark brown lamellae when mature, stipe that is pruinose along the whole length, nodulose, rather small basidiospores (av. 8.8×5.5 μm, Qav.=1.53) and pleurocystidia which are similar to cheilocystidia in shape but smaller in size (19-22 × 47-65 μm). Based on the phylogenetic analysis of nuclear ribosomal ITS and LSU nucleotide sequences I. quercicola belongs into I. sect. Marginatae, subsect. Praetervisae.
Fungal Systematics and Evolution (FUSE) is one of the journal series to address the “fusion” betw... more Fungal Systematics and Evolution (FUSE) is one of the journal series to address the “fusion” between morphological data and molecular phylogenetic data and to describe new fungal taxa and interesting observations. This paper is the 6th contribution in the FUSE series—presenting one new genus, twelve new species, twelve new country records, and three new combinations. The new genus is: Pseudozeugandromyces (Laboulbeniomycetes, Laboulbeniales). The new species are: Albatrellopsis flettioides from Pakistan, Aureoboletus garciae from Mexico, Entomophila canadense from Canada, E. frigidum from Sweden, E. porphyroleucum from Vietnam, Erythrophylloporus flammans from Vietnam, Marasmiellus boreoorientalis from Kamchatka Peninsula in the Russian Far East, Marasmiellus longistipes from Pakistan, Pseudozeugandromyces tachypori on Tachyporus pusillus (Coleoptera, Staphylinidae) from Belgium, Robillarda sohagensis from Egypt, Trechispora hondurensis from Honduras, and Tricholoma kenanii from Tur...
Veloporphyrellus latisporus is proposed as a new species based on morphological characters and su... more Veloporphyrellus latisporus is proposed as a new species based on morphological characters and supporting molecular data from the ITS and LSU regions of the nuclear DNA. The new species is characterized by its red to reddish brown granulose pileus and comparatively broader basidiospores. A key to the species of Veloporphyrellus is provided. This is the first report of any Veloporphyrellus species from Pakistan.
Present study deals with the plectological and molecular analysis as well as use of economically ... more Present study deals with the plectological and molecular analysis as well as use of economically important wild Russuloid mushrooms against food pathogenic fungus Aspergillus niger. Three different species of mushrooms viz., Russla laeta, R. nobilis, and R. nigricans were collected and identified from Himalayan range of Pakistan and are found as new records for this country. Major objective of this study was to highlight the importance of these wild creatures as antifungal agents against A. niger. For this purpose methanolic extract of selected mushrooms of different concentration levels viz., 1, 1.5, 2 and 3% were used. This activity is also first time reported from Pakistan by using this group of mushrooms. Results showed that all tested mushrooms exhibit growth inhibition of A. niger and can be used as biocontrol agents. R. nigricans showed maximum inhibition of fungus growth that is 62% at 3% concentrations while minimum inhibition was observed in R. nobilis at same concentratio...
During this investigation, a new species of Melanoleuca subg. Urticocystis with urticiform cheilo... more During this investigation, a new species of Melanoleuca subg. Urticocystis with urticiform cheilocystidia is proposed. The identification is based on morphological characters and molecular phylogenetic analyses using ITS and 28S sequences. A comparison of the morphological features and molecular phylogenetic data with the taxa of subg. Urticocystis confirms the identity of the species as a new taxon. The detailed descriptions, line drawings and phylogenetic results are given.
This area is a part of the Hindu Kush mountain range and extends west to the great Himalayan rang... more This area is a part of the Hindu Kush mountain range and extends west to the great Himalayan range with an average elevation of 2000 to 3500 m above sea level (a.s.l.) (Ullah et al., 2019b). Climatically, District Shangla belongs to a moist temperate area, and the vegetation resembles Himalayan Mountain vegetation (Ullah et al., 2019a). Almost 90% of the area consists of mountains covered with coniferous and broad-leaved Research Article This work is licensed under a Creative Commons Attribution 4.0 International License.
Amanita subjunquillea and its ectomycorrhizal association are reported for the first time from mo... more Amanita subjunquillea and its ectomycorrhizal association are reported for the first time from moist temperate Himalayan forests of Pakistan. The sample was studied based on morphological characters and nucleotide sequence analyses of the ITS region generated from basidiomata and ectomycorrhizal roots of Quercus floribunda. Our collection differs from the type in its dark orange pileus disc and pale yellow margins. Remaining morphological and molecular data are consistent with previously reported specimens. This represents the first report of A. subjunquillea from Pakistan.
Two new species found in Northwestern Pakistan in the mushroom genus Amanita are described and il... more Two new species found in Northwestern Pakistan in the mushroom genus Amanita are described and illustrated. Phylogenetic data derived from nuclear ribosomal ITS and LSU regions along with morphological characterizations indicate that these species are novel. Amanita cinis is a member of section Roanokenses, while A. olivovaginata is representative of the section Vaginatae. Amanita emodotrygon, recently described from the state of Uttarakhand, India, is new to Pakistan.
Pileolaria pistaciae is a species originally found attacking Pistacia chinensis Bunge in Japan an... more Pileolaria pistaciae is a species originally found attacking Pistacia chinensis Bunge in Japan and a few other Asian countries. In this study, we report the presence of P. pistaciae in Pakistan. Infected leaves of P. chinensis were collected from two different sampling sites in the Abbottabad and Shangla districts of Khyber Pakhtunkhwa province of Pakistan in September-October, 2014. The causal fungus was observed and identified based on combined results of morphological and molecular characterization of the internal transcribed spacer and large subunit regions of ribosomal DNA. This is the first report in Pakistan of Asian Pistachio rust disease caused by P. pistaciae.
Tamarix dioca (L.) belongs to family Tamaricaceae is traditionally a vital plant used for treatme... more Tamarix dioca (L.) belongs to family Tamaricaceae is traditionally a vital plant used for treatment of various diseases. Efforts were made to investigate the antimicrobial efficiency of T. dioca.There was three concentrations of crude methanolic extracts of 200µg/ml, 100µg/ml and 50 µg/ml were checked against six pathogenic fungi Aspergillus fumigatus, aspergillus flavus, Fusarium solani, Aspergillus niger, Penicillium digitatum and Penicillium tubesulum and six bacterial strains Bacillus subtilis, Enterobactor aerogenes, Escherichia coli, Kliebsiella pneumoniae, Salmonella typhi and Staphylococcus aureus. It was noted that Percentage inhibition in the growth of fungi and bacteria was dosage dependent. Terbinafine a standard antifungal drug, 10mg/ml and Cefexime 10mg/ ml (antibacterial) were used as a positive control. The results were compared with control and most of the results were found significant. Maximum inhibition was showed by T. dioca against fungal strain A. niger (74%) and bacterial strain K. pneumoniae (48%). It can be use as powerful antimicrobial agent in near fture.
Crude ethanolic extract of Avicennia marina leaves was tested against seven allergenic fungi viz.... more Crude ethanolic extract of Avicennia marina leaves was tested against seven allergenic fungi viz., Alternaria alternata, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Cladosporium herbarum, Penicillium notatum and Saccharomyces cerevisiae using five different solvents: dimethyl sulphoxide (DMSO), distilled water (DW), chloroform, ethanol and acetone at 2000, 4000 and 6000 ppm doses. Dose dependent tendency in the increase or decrease in the growth of fungi was observed. Two synthetic drugs miconazole and amphotericin-B were used as positive control. Miconazole was 100% effective against A. alternata, C. herbatum, P. notatum and S. cerevisiae with the concentrations of 95.00 ± 1.62, 78.00 ± 4.99, 100.00 ± 0.69 and 110.00 ± 2.33 (µg/ml of SDA medium), respectively. Amphotericin-B completely controlled the growth of A. flavus, A. fumigatus and A. niger in the concentration of 24.00 ± 17.00, 30.00 ± 15.66 and 18.00 ± 18.34 (µg/ml of SDA medium), respectively. Distilled water and DMSO were considered to be the most effective solvents preventing 83.00 ± 4.73%
The present work is a preliminary compilation mostly based on the available literature and extens... more The present work is a preliminary compilation mostly based on the available literature and extensive recent field trips. Overall 46 lithophytic moss species have been reported in Kaghan valley, Mansehra-Pakistan. These species are distributed under 16 families and 28 genera. This study is very much significant regarding the diversity of mosses in the study area that proof the considerable, potential of the ambient environment for the luxurious growth of bryophytes.
The rust fungus, Uromyces ambiens, is described and illustrated based on a specimen of Buxus wall... more The rust fungus, Uromyces ambiens, is described and illustrated based on a specimen of Buxus wallichiana, Himalayan boxwood, from northern Pakistan. This rare fungus is reported for the first time from this host and for the first time from Pakistan.
Aecidium saussureae-affinis and Uredopeltis chevalieri are first time described and illustrated f... more Aecidium saussureae-affinis and Uredopeltis chevalieri are first time described and illustrated from Pakistan and are new records for this area. Grewia optiva, Carpesium trachelifolium and Malus pumila are being reported here as new host records for rust fungi. Telial stage of Phakopsora ziziphi-vulgaris is first time recorded from Pakistan and is an addition to the already reported stages of this rust. Ravenelia taslimii, Tranzschelia discolor and T. pruni-spinosae are additions to the rust flora of Khyber Pakhtunkhwa Province, Pakistan. Introduction Khyber Pakhtunkhwa (KP), formerly known as the Northwest Frontier Province (NWFP), is one of the five provinces of Pakistan, located in the northwest of the country. It is situated at approximately 34.00°N 71.32°E. Khyber Pakhtunkhwa is famous for hill coniferous forests, herbal plants, and large biodiversity. This floristically rich area is affected by many biotic and abiotic stresses including plant diseases such as leaf blights, red...
Urocystis narcissi, reported here from Pakistan, represents a new record for Asia. This smut fung... more Urocystis narcissi, reported here from Pakistan, represents a new record for Asia. This smut fungus was collected for the first time outside its type locality in Spain. Narcissus tazetta is recognized as a new host plant for this rare smut.
This paper deals with the taxonomic studies of the family Bartramiaceae, Bryophyta, from Mansehra... more This paper deals with the taxonomic studies of the family Bartramiaceae, Bryophyta, from Mansehra district, Khyber Pakhtoonkhaw province, Pakistan. Extensive field surveys were conducted from October 2012 to December 2014. Previously collected specimens hosted at Pakistan Museum of Natural History (PMNH) were also consulted. Keys (at generic and specific level), description, general distribution and foot notes have been provided. Six species were recognized in this study from the area.
Basidiomata of Macrolepiota dolichaula and M. excoriata were collected from mixed coniferous fore... more Basidiomata of Macrolepiota dolichaula and M. excoriata were collected from mixed coniferous forests in Pakistan. The species were identified using ITS nrDNA sequence analyses and morphological and anatomical characters. Detailed morphological and anatomical descriptions and illustrations are provided along with results of molecular phylogenetic analysis. Both species are regarded as new records for Pakistan, and this report considerably extends their known distribution.
The new species Inocybe quercicola is described on the basis of morphological and genetic feature... more The new species Inocybe quercicola is described on the basis of morphological and genetic features. It is characterized by medium-sized basidiomata, radially fibrillose campanulate pileus, dark brown lamellae when mature, stipe that is pruinose along the whole length, nodulose, rather small basidiospores (av. 8.8×5.5 μm, Qav.=1.53) and pleurocystidia which are similar to cheilocystidia in shape but smaller in size (19-22 × 47-65 μm). Based on the phylogenetic analysis of nuclear ribosomal ITS and LSU nucleotide sequences I. quercicola belongs into I. sect. Marginatae, subsect. Praetervisae.
Fungal Systematics and Evolution (FUSE) is one of the journal series to address the “fusion” betw... more Fungal Systematics and Evolution (FUSE) is one of the journal series to address the “fusion” between morphological data and molecular phylogenetic data and to describe new fungal taxa and interesting observations. This paper is the 6th contribution in the FUSE series—presenting one new genus, twelve new species, twelve new country records, and three new combinations. The new genus is: Pseudozeugandromyces (Laboulbeniomycetes, Laboulbeniales). The new species are: Albatrellopsis flettioides from Pakistan, Aureoboletus garciae from Mexico, Entomophila canadense from Canada, E. frigidum from Sweden, E. porphyroleucum from Vietnam, Erythrophylloporus flammans from Vietnam, Marasmiellus boreoorientalis from Kamchatka Peninsula in the Russian Far East, Marasmiellus longistipes from Pakistan, Pseudozeugandromyces tachypori on Tachyporus pusillus (Coleoptera, Staphylinidae) from Belgium, Robillarda sohagensis from Egypt, Trechispora hondurensis from Honduras, and Tricholoma kenanii from Tur...
Veloporphyrellus latisporus is proposed as a new species based on morphological characters and su... more Veloporphyrellus latisporus is proposed as a new species based on morphological characters and supporting molecular data from the ITS and LSU regions of the nuclear DNA. The new species is characterized by its red to reddish brown granulose pileus and comparatively broader basidiospores. A key to the species of Veloporphyrellus is provided. This is the first report of any Veloporphyrellus species from Pakistan.
Present study deals with the plectological and molecular analysis as well as use of economically ... more Present study deals with the plectological and molecular analysis as well as use of economically important wild Russuloid mushrooms against food pathogenic fungus Aspergillus niger. Three different species of mushrooms viz., Russla laeta, R. nobilis, and R. nigricans were collected and identified from Himalayan range of Pakistan and are found as new records for this country. Major objective of this study was to highlight the importance of these wild creatures as antifungal agents against A. niger. For this purpose methanolic extract of selected mushrooms of different concentration levels viz., 1, 1.5, 2 and 3% were used. This activity is also first time reported from Pakistan by using this group of mushrooms. Results showed that all tested mushrooms exhibit growth inhibition of A. niger and can be used as biocontrol agents. R. nigricans showed maximum inhibition of fungus growth that is 62% at 3% concentrations while minimum inhibition was observed in R. nobilis at same concentratio...
During this investigation, a new species of Melanoleuca subg. Urticocystis with urticiform cheilo... more During this investigation, a new species of Melanoleuca subg. Urticocystis with urticiform cheilocystidia is proposed. The identification is based on morphological characters and molecular phylogenetic analyses using ITS and 28S sequences. A comparison of the morphological features and molecular phylogenetic data with the taxa of subg. Urticocystis confirms the identity of the species as a new taxon. The detailed descriptions, line drawings and phylogenetic results are given.
This area is a part of the Hindu Kush mountain range and extends west to the great Himalayan rang... more This area is a part of the Hindu Kush mountain range and extends west to the great Himalayan range with an average elevation of 2000 to 3500 m above sea level (a.s.l.) (Ullah et al., 2019b). Climatically, District Shangla belongs to a moist temperate area, and the vegetation resembles Himalayan Mountain vegetation (Ullah et al., 2019a). Almost 90% of the area consists of mountains covered with coniferous and broad-leaved Research Article This work is licensed under a Creative Commons Attribution 4.0 International License.
Amanita subjunquillea and its ectomycorrhizal association are reported for the first time from mo... more Amanita subjunquillea and its ectomycorrhizal association are reported for the first time from moist temperate Himalayan forests of Pakistan. The sample was studied based on morphological characters and nucleotide sequence analyses of the ITS region generated from basidiomata and ectomycorrhizal roots of Quercus floribunda. Our collection differs from the type in its dark orange pileus disc and pale yellow margins. Remaining morphological and molecular data are consistent with previously reported specimens. This represents the first report of A. subjunquillea from Pakistan.
Two new species found in Northwestern Pakistan in the mushroom genus Amanita are described and il... more Two new species found in Northwestern Pakistan in the mushroom genus Amanita are described and illustrated. Phylogenetic data derived from nuclear ribosomal ITS and LSU regions along with morphological characterizations indicate that these species are novel. Amanita cinis is a member of section Roanokenses, while A. olivovaginata is representative of the section Vaginatae. Amanita emodotrygon, recently described from the state of Uttarakhand, India, is new to Pakistan.
Pileolaria pistaciae is a species originally found attacking Pistacia chinensis Bunge in Japan an... more Pileolaria pistaciae is a species originally found attacking Pistacia chinensis Bunge in Japan and a few other Asian countries. In this study, we report the presence of P. pistaciae in Pakistan. Infected leaves of P. chinensis were collected from two different sampling sites in the Abbottabad and Shangla districts of Khyber Pakhtunkhwa province of Pakistan in September-October, 2014. The causal fungus was observed and identified based on combined results of morphological and molecular characterization of the internal transcribed spacer and large subunit regions of ribosomal DNA. This is the first report in Pakistan of Asian Pistachio rust disease caused by P. pistaciae.
Tamarix dioca (L.) belongs to family Tamaricaceae is traditionally a vital plant used for treatme... more Tamarix dioca (L.) belongs to family Tamaricaceae is traditionally a vital plant used for treatment of various diseases. Efforts were made to investigate the antimicrobial efficiency of T. dioca.There was three concentrations of crude methanolic extracts of 200µg/ml, 100µg/ml and 50 µg/ml were checked against six pathogenic fungi Aspergillus fumigatus, aspergillus flavus, Fusarium solani, Aspergillus niger, Penicillium digitatum and Penicillium tubesulum and six bacterial strains Bacillus subtilis, Enterobactor aerogenes, Escherichia coli, Kliebsiella pneumoniae, Salmonella typhi and Staphylococcus aureus. It was noted that Percentage inhibition in the growth of fungi and bacteria was dosage dependent. Terbinafine a standard antifungal drug, 10mg/ml and Cefexime 10mg/ ml (antibacterial) were used as a positive control. The results were compared with control and most of the results were found significant. Maximum inhibition was showed by T. dioca against fungal strain A. niger (74%) and bacterial strain K. pneumoniae (48%). It can be use as powerful antimicrobial agent in near fture.
Crude ethanolic extract of Avicennia marina leaves was tested against seven allergenic fungi viz.... more Crude ethanolic extract of Avicennia marina leaves was tested against seven allergenic fungi viz., Alternaria alternata, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Cladosporium herbarum, Penicillium notatum and Saccharomyces cerevisiae using five different solvents: dimethyl sulphoxide (DMSO), distilled water (DW), chloroform, ethanol and acetone at 2000, 4000 and 6000 ppm doses. Dose dependent tendency in the increase or decrease in the growth of fungi was observed. Two synthetic drugs miconazole and amphotericin-B were used as positive control. Miconazole was 100% effective against A. alternata, C. herbatum, P. notatum and S. cerevisiae with the concentrations of 95.00 ± 1.62, 78.00 ± 4.99, 100.00 ± 0.69 and 110.00 ± 2.33 (µg/ml of SDA medium), respectively. Amphotericin-B completely controlled the growth of A. flavus, A. fumigatus and A. niger in the concentration of 24.00 ± 17.00, 30.00 ± 15.66 and 18.00 ± 18.34 (µg/ml of SDA medium), respectively. Distilled water and DMSO were considered to be the most effective solvents preventing 83.00 ± 4.73%
The present work is a preliminary compilation mostly based on the available literature and extens... more The present work is a preliminary compilation mostly based on the available literature and extensive recent field trips. Overall 46 lithophytic moss species have been reported in Kaghan valley, Mansehra-Pakistan. These species are distributed under 16 families and 28 genera. This study is very much significant regarding the diversity of mosses in the study area that proof the considerable, potential of the ambient environment for the luxurious growth of bryophytes.
The rust fungus, Uromyces ambiens, is described and illustrated based on a specimen of Buxus wall... more The rust fungus, Uromyces ambiens, is described and illustrated based on a specimen of Buxus wallichiana, Himalayan boxwood, from northern Pakistan. This rare fungus is reported for the first time from this host and for the first time from Pakistan.
Aecidium saussureae-affinis and Uredopeltis chevalieri are first time described and illustrated f... more Aecidium saussureae-affinis and Uredopeltis chevalieri are first time described and illustrated from Pakistan and are new records for this area. Grewia optiva, Carpesium trachelifolium and Malus pumila are being reported here as new host records for rust fungi. Telial stage of Phakopsora ziziphi-vulgaris is first time recorded from Pakistan and is an addition to the already reported stages of this rust. Ravenelia taslimii, Tranzschelia discolor and T. pruni-spinosae are additions to the rust flora of Khyber Pakhtunkhwa Province, Pakistan. Introduction Khyber Pakhtunkhwa (KP), formerly known as the Northwest Frontier Province (NWFP), is one of the five provinces of Pakistan, located in the northwest of the country. It is situated at approximately 34.00°N 71.32°E. Khyber Pakhtunkhwa is famous for hill coniferous forests, herbal plants, and large biodiversity. This floristically rich area is affected by many biotic and abiotic stresses including plant diseases such as leaf blights, red...
Urocystis narcissi, reported here from Pakistan, represents a new record for Asia. This smut fung... more Urocystis narcissi, reported here from Pakistan, represents a new record for Asia. This smut fungus was collected for the first time outside its type locality in Spain. Narcissus tazetta is recognized as a new host plant for this rare smut.
This paper deals with the taxonomic studies of the family Bartramiaceae, Bryophyta, from Mansehra... more This paper deals with the taxonomic studies of the family Bartramiaceae, Bryophyta, from Mansehra district, Khyber Pakhtoonkhaw province, Pakistan. Extensive field surveys were conducted from October 2012 to December 2014. Previously collected specimens hosted at Pakistan Museum of Natural History (PMNH) were also consulted. Keys (at generic and specific level), description, general distribution and foot notes have been provided. Six species were recognized in this study from the area.
Basidiomata of Macrolepiota dolichaula and M. excoriata were collected from mixed coniferous fore... more Basidiomata of Macrolepiota dolichaula and M. excoriata were collected from mixed coniferous forests in Pakistan. The species were identified using ITS nrDNA sequence analyses and morphological and anatomical characters. Detailed morphological and anatomical descriptions and illustrations are provided along with results of molecular phylogenetic analysis. Both species are regarded as new records for Pakistan, and this report considerably extends their known distribution.
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