The U.S. Global Change Research Program reports that the frequency and intensity of extreme heat ... more The U.S. Global Change Research Program reports that the frequency and intensity of extreme heat are increasing globally. Studies of the impact of climate change on child health often exclude sleep, despite its importance for healthy growth and development. To address this gap in the literature, we studied the impact of unusually high temperatures in the summer of 2022 on infants’ sleep. Sleep was assessed objectively using Nanit camera monitors in infants’ homes. Generally, sleep was not impacted when temperatures stayed below 88° but was negatively impacted when temperatures reached over 100°. Compared to non-heatwave nights, infants had less total sleep, less efficient sleep, took longer to fall asleep, had more fragmented sleep, and parents’ visits were more frequent during the night. Following peaks in temperature, sleep metrics rebounded to better than average compared to non-peak nights, suggesting that infants compensated for disrupted sleep by sleeping more and with fewer i...
Journal of Clinical and Translational Science, 2017
negatively correlated with Pax5 expression (r=0.9993; p=0.0167). We next confirmed the expression... more negatively correlated with Pax5 expression (r=0.9993; p=0.0167). We next confirmed the expression patterns of ETV6 and PAX5 during B cell development in human samples. We found that ETV6 expression was higher in the early B cell fraction (CD10+ , CD34+ , CD19− , and CD20− ) compared to the pre-B cell fraction (CD10+ , CD34− , CD19+ , CD20− ). Conversely, we observed that PAX5 expression was higher in the preB cell fraction compared with the early B cell fraction. In Ba/F3 cells expressing ETV6 constructs, ETV6, but not ETV6 P214L overexpression significantly decreased Pax5 expression (p≤0.05). ETV6 is associated with the proximal GGAA site 72 base pairs upstream of the Pax5 TSS, but not GGAA sites further from the TSS. In addition, the transcriptional repressors SIN3A and HDAC3 were detected on the same regions of the Pax5 locus. We detected association of ETV6, SIN3A, and HDAC3 with the proximal GGAA site upon expression of WT ETV6, but not ETV6 P214L. DISCUSSION/ SIGNIFICANCEOF IM...
Introduction: Perceived racial discrimination has been associated with dysregulation of sympathet... more Introduction: Perceived racial discrimination has been associated with dysregulation of sympathetic-adrenal-medullary (SAM) and hypothalamic-pituitary-adrenal (HPA) axis, two major physiological stress response systems linked to cardiovascular disease outcomes. Most studies, to date, used cross-sectional data that captured retrospective measures of lifetime racial discrimination associated with current physiological stress responses. Relative to overt racial discrimination, a subtle form of racial discrimination, known as microaggression is more prevalent, yet most commonly measured retrospectively, and understudied. The purpose of this pilot study was to examine the relationship between microaggression measured in real-time and physiological stress responses. Methods: Twelve healthy Black adults (mean age=43.4 years) completed baseline surveys, and self-collected saliva 4x a day for 4 days to measure cortisol and alpha amylase (AA) as a proxy of HPA and SAM systems, respectively. R...
Journal of clinical and translational science, Sep 1, 2017
thereafter (p < 0.0001). Etv6 expression decreases as B cells develop and is negatively correlate... more thereafter (p < 0.0001). Etv6 expression decreases as B cells develop and is negatively correlated with Pax5 expression (r 2 = 0.9993; p = 0.0167). We next confirmed the expression patterns of ETV6 and PAX5 during B cell development in human samples. We found that ETV6 expression was higher in the early B cell fraction (CD10+ , CD34+ , CD19− , and CD20−) compared to the pre-B cell fraction (CD10+ , CD34 − , CD19+ , CD20−). Conversely, we observed that PAX5 expression was higher in the preB cell fraction compared with the early B cell fraction. In Ba/F3 cells expressing ETV6 constructs, ETV6, but not ETV6 P214L overexpression significantly decreased Pax5 expression (p ≤ 0.05). ETV6 is associated with the proximal GGAA site 72 base pairs upstream of the Pax5 TSS, but not GGAA sites further from the TSS. In addition, the transcriptional repressors SIN3A and HDAC3 were detected on the same regions of the Pax5 locus. We detected association of ETV6, SIN3A, and HDAC3 with the proximal GGAA site upon expression of WT ETV6, but not ETV6 P214L. DISCUSSION/ SIGNIFICANCE OF IMPACT: Our results provide a mechanism of interaction for ETV6 and PAX5, 2 genes often disrupted in B-cell leukemia. These findings are significant because PAX5 misregulation results in a B cell development halt, lineage infidelity, and leukemogenesis. In continuing our studies, we have generated a transgenic mouse endogenously expressing the ETV6 P214L mutation by CRISPR/ Cas9 editing, and these mice appear to have a thrombocytopenic phenotype similar to that observed in patients carrying the ETV6 P214L mutation. These animals will be the focus of our continued investigation of the mechanism by which ETV6 germline mutation results in a predisposition to leukemia. Our ultimate goal is a comprehensive understanding of how this process may be targeted more efficiently in patients with both heritable and sporadic forms of leukemia involving ETV6.
Problem: In recent years, there has been an increase in research studying the effects of elementa... more Problem: In recent years, there has been an increase in research studying the effects of elemental lead on women during lactation. In order to enhance the clinical utility of the findings from the individual studies, they must be integrated. Method: The following terms or combination of terms were used to conduct the article search: lead poisoning, plumbism, lactation, and breastfeeding. The computerized databases of MEDLINE and CINAHL were used to conduct the search. Findings: Sixteen articles were included in this review. Findings revealed there were significantly larger increases in the mobilization of skeletal lead during the postpartum period than during pregnancy. The major sources of lead in breast milk were found to be from maternal bone and maternal diet. Breast milk lead levels were noted to significantly decrease over the course of lactation. Breast milk lead levels were shown to have a strong influence on infant blood levels over and above the influence of maternal blood...
Mothers with depressive symptoms more frequently report behavioral problems among their children ... more Mothers with depressive symptoms more frequently report behavioral problems among their children than non-depressed mothers leading to a debate regarding the accuracy of depressed mothers as informants of children's behavior. The purpose of this integrative review was to identify methodological challenges in research related to the debate. Data were extracted from 43 papers (6 theoretical, 36 research reports, and 1 instrument scoring manual). The analysis focused on the methodologies considered when using depressed mothers as informants. Nine key themes were identified and I concluded that researchers should incorporate multiple informants, identify the characteristics of maternal depression, and incorporate advanced statistical methodology. The use of a conceptual framework to understand informant discrepancies within child behavior evaluations is suggested for future research. Depressed mothers; child behavior; informants; methodological issues Children of depressed mothers are more frequently reported to have behavioral problems . In reports of studies in which hierarchical regression analysis with multiple maternal variables was used, self-reported maternal depressive symptoms have been found to explain the most variance in children's behaviors . Depression in mothers has been associated with negative maternal cognitions, behaviors, and affect that limit maternal functioning and may therefore increase children's behavior problems . Children of depressed mothers are reportedly often left with unmet needs that affect their development because their mothers are unable to meet their social and emotional needs . Although understanding the effects of maternal depression on child behavior has been the impetus behind much behavioral research , the mechanisms by which maternal depression influences child behavior have not been thoroughly examined and are the subject of current debate . In a frequently cited article, Richters (1992) proposed two opposing models regarding the influence of maternal depression on informant reliability. In the distortion model, depressed mothers are said to over-report child behavior
Every parent and child would greatly benefit from an intervention that would ensure improved immu... more Every parent and child would greatly benefit from an intervention that would ensure improved immunity reduced illnesses of varying types reduced cancer risk and improved cognitive function. So when a health care provider explains that this particular intervention is not an artificial laboratory-created pharmacological agent but rather the natural benefits of breast milk why do so few women make the decision to breastfeed? Primary influences that affect a womans decisions include her support structure parity prepared knowledge practical work demands and level of comfort with public breastfeeding. Further research must examine how to synthesize studies that show the multifaceted influences on a womans decision to breastfeed. This integration will clarify why 2 women in apparently the same situation (similar culture age support structure etc) make different decisions regarding the continuation of breastfeeding. One way to do this is to categorize women using the model by Belenky Clinchy Goldberger and Tarule based on their 1997 book Womens Ways of Knowing. (excerpt)
Aim-To identify the definitional elements of parental reflective functioning (RF) and develop a f... more Aim-To identify the definitional elements of parental reflective functioning (RF) and develop a framework for nurses to apply this concept in their clinical work with families. Background: In recent years, researchers have concluded that parental RF is a key mechanism in the development of child attachment security leading to lifelong mental and physical health benefits. Despite its clinical relevance, little has been published in the nursing literature on this concept. Design-Concept analysis. Methods-The Walker and Avant (2011) method of concept analysis and the Whittemore and Knafl (2005) method of integrative review were used. A search of the literature published from 1989 to 2013 was conducted using edited texts and online databases-Scopus, CINAHL, PubMed,
The U.S. Global Change Research Program reports that the frequency and intensity of extreme heat ... more The U.S. Global Change Research Program reports that the frequency and intensity of extreme heat are increasing globally. Studies of the impact of climate change on child health often exclude sleep, despite its importance for healthy growth and development. To address this gap in the literature, we studied the impact of unusually high temperatures in the summer of 2022 on infants’ sleep. Sleep was assessed objectively using Nanit camera monitors in infants’ homes. Generally, sleep was not impacted when temperatures stayed below 88° but was negatively impacted when temperatures reached over 100°. Compared to non-heatwave nights, infants had less total sleep, less efficient sleep, took longer to fall asleep, had more fragmented sleep, and parents’ visits were more frequent during the night. Following peaks in temperature, sleep metrics rebounded to better than average compared to non-peak nights, suggesting that infants compensated for disrupted sleep by sleeping more and with fewer i...
Journal of Clinical and Translational Science, 2017
negatively correlated with Pax5 expression (r=0.9993; p=0.0167). We next confirmed the expression... more negatively correlated with Pax5 expression (r=0.9993; p=0.0167). We next confirmed the expression patterns of ETV6 and PAX5 during B cell development in human samples. We found that ETV6 expression was higher in the early B cell fraction (CD10+ , CD34+ , CD19− , and CD20− ) compared to the pre-B cell fraction (CD10+ , CD34− , CD19+ , CD20− ). Conversely, we observed that PAX5 expression was higher in the preB cell fraction compared with the early B cell fraction. In Ba/F3 cells expressing ETV6 constructs, ETV6, but not ETV6 P214L overexpression significantly decreased Pax5 expression (p≤0.05). ETV6 is associated with the proximal GGAA site 72 base pairs upstream of the Pax5 TSS, but not GGAA sites further from the TSS. In addition, the transcriptional repressors SIN3A and HDAC3 were detected on the same regions of the Pax5 locus. We detected association of ETV6, SIN3A, and HDAC3 with the proximal GGAA site upon expression of WT ETV6, but not ETV6 P214L. DISCUSSION/ SIGNIFICANCEOF IM...
Introduction: Perceived racial discrimination has been associated with dysregulation of sympathet... more Introduction: Perceived racial discrimination has been associated with dysregulation of sympathetic-adrenal-medullary (SAM) and hypothalamic-pituitary-adrenal (HPA) axis, two major physiological stress response systems linked to cardiovascular disease outcomes. Most studies, to date, used cross-sectional data that captured retrospective measures of lifetime racial discrimination associated with current physiological stress responses. Relative to overt racial discrimination, a subtle form of racial discrimination, known as microaggression is more prevalent, yet most commonly measured retrospectively, and understudied. The purpose of this pilot study was to examine the relationship between microaggression measured in real-time and physiological stress responses. Methods: Twelve healthy Black adults (mean age=43.4 years) completed baseline surveys, and self-collected saliva 4x a day for 4 days to measure cortisol and alpha amylase (AA) as a proxy of HPA and SAM systems, respectively. R...
Journal of clinical and translational science, Sep 1, 2017
thereafter (p < 0.0001). Etv6 expression decreases as B cells develop and is negatively correlate... more thereafter (p < 0.0001). Etv6 expression decreases as B cells develop and is negatively correlated with Pax5 expression (r 2 = 0.9993; p = 0.0167). We next confirmed the expression patterns of ETV6 and PAX5 during B cell development in human samples. We found that ETV6 expression was higher in the early B cell fraction (CD10+ , CD34+ , CD19− , and CD20−) compared to the pre-B cell fraction (CD10+ , CD34 − , CD19+ , CD20−). Conversely, we observed that PAX5 expression was higher in the preB cell fraction compared with the early B cell fraction. In Ba/F3 cells expressing ETV6 constructs, ETV6, but not ETV6 P214L overexpression significantly decreased Pax5 expression (p ≤ 0.05). ETV6 is associated with the proximal GGAA site 72 base pairs upstream of the Pax5 TSS, but not GGAA sites further from the TSS. In addition, the transcriptional repressors SIN3A and HDAC3 were detected on the same regions of the Pax5 locus. We detected association of ETV6, SIN3A, and HDAC3 with the proximal GGAA site upon expression of WT ETV6, but not ETV6 P214L. DISCUSSION/ SIGNIFICANCE OF IMPACT: Our results provide a mechanism of interaction for ETV6 and PAX5, 2 genes often disrupted in B-cell leukemia. These findings are significant because PAX5 misregulation results in a B cell development halt, lineage infidelity, and leukemogenesis. In continuing our studies, we have generated a transgenic mouse endogenously expressing the ETV6 P214L mutation by CRISPR/ Cas9 editing, and these mice appear to have a thrombocytopenic phenotype similar to that observed in patients carrying the ETV6 P214L mutation. These animals will be the focus of our continued investigation of the mechanism by which ETV6 germline mutation results in a predisposition to leukemia. Our ultimate goal is a comprehensive understanding of how this process may be targeted more efficiently in patients with both heritable and sporadic forms of leukemia involving ETV6.
Problem: In recent years, there has been an increase in research studying the effects of elementa... more Problem: In recent years, there has been an increase in research studying the effects of elemental lead on women during lactation. In order to enhance the clinical utility of the findings from the individual studies, they must be integrated. Method: The following terms or combination of terms were used to conduct the article search: lead poisoning, plumbism, lactation, and breastfeeding. The computerized databases of MEDLINE and CINAHL were used to conduct the search. Findings: Sixteen articles were included in this review. Findings revealed there were significantly larger increases in the mobilization of skeletal lead during the postpartum period than during pregnancy. The major sources of lead in breast milk were found to be from maternal bone and maternal diet. Breast milk lead levels were noted to significantly decrease over the course of lactation. Breast milk lead levels were shown to have a strong influence on infant blood levels over and above the influence of maternal blood...
Mothers with depressive symptoms more frequently report behavioral problems among their children ... more Mothers with depressive symptoms more frequently report behavioral problems among their children than non-depressed mothers leading to a debate regarding the accuracy of depressed mothers as informants of children's behavior. The purpose of this integrative review was to identify methodological challenges in research related to the debate. Data were extracted from 43 papers (6 theoretical, 36 research reports, and 1 instrument scoring manual). The analysis focused on the methodologies considered when using depressed mothers as informants. Nine key themes were identified and I concluded that researchers should incorporate multiple informants, identify the characteristics of maternal depression, and incorporate advanced statistical methodology. The use of a conceptual framework to understand informant discrepancies within child behavior evaluations is suggested for future research. Depressed mothers; child behavior; informants; methodological issues Children of depressed mothers are more frequently reported to have behavioral problems . In reports of studies in which hierarchical regression analysis with multiple maternal variables was used, self-reported maternal depressive symptoms have been found to explain the most variance in children's behaviors . Depression in mothers has been associated with negative maternal cognitions, behaviors, and affect that limit maternal functioning and may therefore increase children's behavior problems . Children of depressed mothers are reportedly often left with unmet needs that affect their development because their mothers are unable to meet their social and emotional needs . Although understanding the effects of maternal depression on child behavior has been the impetus behind much behavioral research , the mechanisms by which maternal depression influences child behavior have not been thoroughly examined and are the subject of current debate . In a frequently cited article, Richters (1992) proposed two opposing models regarding the influence of maternal depression on informant reliability. In the distortion model, depressed mothers are said to over-report child behavior
Every parent and child would greatly benefit from an intervention that would ensure improved immu... more Every parent and child would greatly benefit from an intervention that would ensure improved immunity reduced illnesses of varying types reduced cancer risk and improved cognitive function. So when a health care provider explains that this particular intervention is not an artificial laboratory-created pharmacological agent but rather the natural benefits of breast milk why do so few women make the decision to breastfeed? Primary influences that affect a womans decisions include her support structure parity prepared knowledge practical work demands and level of comfort with public breastfeeding. Further research must examine how to synthesize studies that show the multifaceted influences on a womans decision to breastfeed. This integration will clarify why 2 women in apparently the same situation (similar culture age support structure etc) make different decisions regarding the continuation of breastfeeding. One way to do this is to categorize women using the model by Belenky Clinchy Goldberger and Tarule based on their 1997 book Womens Ways of Knowing. (excerpt)
Aim-To identify the definitional elements of parental reflective functioning (RF) and develop a f... more Aim-To identify the definitional elements of parental reflective functioning (RF) and develop a framework for nurses to apply this concept in their clinical work with families. Background: In recent years, researchers have concluded that parental RF is a key mechanism in the development of child attachment security leading to lifelong mental and physical health benefits. Despite its clinical relevance, little has been published in the nursing literature on this concept. Design-Concept analysis. Methods-The Walker and Avant (2011) method of concept analysis and the Whittemore and Knafl (2005) method of integrative review were used. A search of the literature published from 1989 to 2013 was conducted using edited texts and online databases-Scopus, CINAHL, PubMed,
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Papers by Monica Ordway