This study aimed to determine whether TNF-α is transferred to equine neonates via colostrum and t... more This study aimed to determine whether TNF-α is transferred to equine neonates via colostrum and the relationship between TNF-α and IgG concentrations in the equine neonate. Colostrum, presuckle and postsuckle foal serum samples were collected from healthy mares and their foals. Equine TNF-α ELISA and IgG SRID kits were used to determine the concentrations of TNF-α and IgG, respectively. Statistical analysis was performed using the Spearman rank correlation. TNF-α concentrations in all presuckle foal serum were below the limit of detection in 15/16 foals and increased in postsuckle foal serum to a mean concentration of 7.7 x 10(4) pg/ml. TNF-α concentrations in postsuckle foal serum and colostrum showed significant correlation (rho=0.668; P=0.005). However, TNF-α and IgG concentrations in colostrum or postsuckle foal serum did not correlate (rho<-0.016; P>0.05). Ratios of TNF-α/IgG in colostrum or postsuckle foal serum showed significant correlation (rho=0.750; P=0.0008). These results indicate that TNF-α is transferred to the foal via colostrum absorption and may play a role in early immunity.
A Thoroughbred gelding in North America was evaluated for Actinobacillus peritonitis on three dif... more A Thoroughbred gelding in North America was evaluated for Actinobacillus peritonitis on three different occasions over a 4-year period. At each presentation, peritoneal fluid had an elevated nucleated cell count (220,000-550,000 cells/mL) characterised by non-degenerate neutrophils, no visible bacteria, an elevated total protein (4.6-5.5 g/dL) and bacterial culture yielding Actinobacillus spp. Actinobacillus peritonitis appears to be a regional disease occurring in Australia and less commonly in New Zealand and North America. Recurrence, other than incomplete resolution, has not been previously reported. This case highlights the classical presentation, response to therapy and excellent prognosis despite the alarmingly abnormal peritoneal fluid characteristic of Actinobacillus peritonitis and questions the role of parasite migration in the pathogenesis. Finally, this case is remarkable because Actinobacillus peritonitis was recurrent over several years in an otherwise normal horse.
To evaluate connective tissue reactions to iRoot SP (Innovative Bioceramics, Vancouver, BC, Canad... more To evaluate connective tissue reactions to iRoot SP (Innovative Bioceramics, Vancouver, BC, Canada), mineral trioxide aggregate (MTA) Fillapex (FLPX) (Angelus Soluções Odontológicas, Londrina, Brazil), DiaRoot Bioaggregate (DiaDent Group International, Burnaby, BC, Canada) and white MTA (Angelus, Londrina, Brazil) in Wistar rats. A total of 128 dentine tubes filled with the materials and 32 empty tubes (control) were implanted into 32 rats. After 7, 15, 30 and 90 days (n = 8 per period), the animals were euthanized, and the tissues were processed for histological evaluation using haematoxylin-eosin (H&E) and Von Kossa (VK) staining. Observations were made for cellular inflammatory components and the presence of multinucleated giant cells (MNGC), macrophages and tissue necrosis. Data were analysed by Fisher's exact and Kruskal–Wallis tests (P < 0.05). In all experimental periods, MTA FLPX and iRoot SP scored higher than the other groups for the variable macrophages (P < 0.05). After 30- and 90-day experimental periods, MTA FLPX scored higher than the other groups for the variable MNGC (P < 0.05). After 90 days, the only group that exhibited samples with severe inflammatory response was MTA FLPX. VK positivity was observed in areas of necrosis in all groups, except in the control group. The materials were considered biologically acceptable except MTA FLPX, which remained toxic to subcutaneous tissue even after 90 days.
Felippe WT, Felippe MCS, Rocha MJC. The effect of mineral trioxide aggregate on the apexification... more Felippe WT, Felippe MCS, Rocha MJC. The effect of mineral trioxide aggregate on the apexification and periapical healing of teeth with incomplete root formation. International Endodontic Journal, 39, 2-9, 2006.
dos Santos LGP, Felippe WT, Teixeira CS, Bortoluzzi EA, Felippe MCS. Endodontic re-instrumentatio... more dos Santos LGP, Felippe WT, Teixeira CS, Bortoluzzi EA, Felippe MCS. Endodontic re-instrumentation enhances hydroxyl ion diffusion through radicular dentine. International Endodontic Journal, 47, 776-783, 2014. Aim To evaluate the diffusion of hydroxyl ions from calcium hydroxide paste (CH) before root canal filling and after retreatment. Methodology After preparation of 60 root canals, the cementum layer was removed, and the canals and root surfaces were treated for smear layer removal. The apical third of roots was covered with adhesive. The canals were filled with CH, and the teeth were placed in individual vials containing 10 mL of distilled water, which had its pH measured after 7, 14, 21 and 28 days (pH1). The root canals were then divided into five groups and filled with Resilon/Real Seal (G1) or gutta-percha and Endofill (G2), Sealapex (G3), AH Plus (G4) or MTA Fillapex (G5) sealers. After storage for 7 days, the root canals were retreated. The CH was again inserted into the canals, and the teeth were placed in new vials containing 10 mL of distilled water. The pH was again measured at 7, 14, 21 and 28 days (pH2). The initial and final pH readings (pH1 and pH2) were compared by ANOVA, ANOVA2 and Tukey's tests (P < 0.05). Results The pH1 and pH2 measurements increased with time. The measurements obtained after retreatment were significantly higher than those obtained before root canal filling. Conclusions Hydroxyl ions are able to diffuse through dentinal tubules. Regardless of the filling material, it was possible to re-establish the permeability of dentine to ionic diffusion after retreatment. Time had a positive influence on ionic diffusion.
This study aimed to determine whether TNF-α is transferred to equine neonates via colostrum and t... more This study aimed to determine whether TNF-α is transferred to equine neonates via colostrum and the relationship between TNF-α and IgG concentrations in the equine neonate. Colostrum, presuckle and postsuckle foal serum samples were collected from healthy mares and their foals. Equine TNF-α ELISA and IgG SRID kits were used to determine the concentrations of TNF-α and IgG, respectively. Statistical analysis was performed using the Spearman rank correlation. TNF-α concentrations in all presuckle foal serum were below the limit of detection in 15/16 foals and increased in postsuckle foal serum to a mean concentration of 7.7 x 10(4) pg/ml. TNF-α concentrations in postsuckle foal serum and colostrum showed significant correlation (rho=0.668; P=0.005). However, TNF-α and IgG concentrations in colostrum or postsuckle foal serum did not correlate (rho&lt;-0.016; P&gt;0.05). Ratios of TNF-α/IgG in colostrum or postsuckle foal serum showed significant correlation (rho=0.750; P=0.0008). These results indicate that TNF-α is transferred to the foal via colostrum absorption and may play a role in early immunity.
A Thoroughbred gelding in North America was evaluated for Actinobacillus peritonitis on three dif... more A Thoroughbred gelding in North America was evaluated for Actinobacillus peritonitis on three different occasions over a 4-year period. At each presentation, peritoneal fluid had an elevated nucleated cell count (220,000-550,000 cells/mL) characterised by non-degenerate neutrophils, no visible bacteria, an elevated total protein (4.6-5.5 g/dL) and bacterial culture yielding Actinobacillus spp. Actinobacillus peritonitis appears to be a regional disease occurring in Australia and less commonly in New Zealand and North America. Recurrence, other than incomplete resolution, has not been previously reported. This case highlights the classical presentation, response to therapy and excellent prognosis despite the alarmingly abnormal peritoneal fluid characteristic of Actinobacillus peritonitis and questions the role of parasite migration in the pathogenesis. Finally, this case is remarkable because Actinobacillus peritonitis was recurrent over several years in an otherwise normal horse.
To evaluate connective tissue reactions to iRoot SP (Innovative Bioceramics, Vancouver, BC, Canad... more To evaluate connective tissue reactions to iRoot SP (Innovative Bioceramics, Vancouver, BC, Canada), mineral trioxide aggregate (MTA) Fillapex (FLPX) (Angelus Soluções Odontológicas, Londrina, Brazil), DiaRoot Bioaggregate (DiaDent Group International, Burnaby, BC, Canada) and white MTA (Angelus, Londrina, Brazil) in Wistar rats. A total of 128 dentine tubes filled with the materials and 32 empty tubes (control) were implanted into 32 rats. After 7, 15, 30 and 90 days (n = 8 per period), the animals were euthanized, and the tissues were processed for histological evaluation using haematoxylin-eosin (H&amp;E) and Von Kossa (VK) staining. Observations were made for cellular inflammatory components and the presence of multinucleated giant cells (MNGC), macrophages and tissue necrosis. Data were analysed by Fisher&#39;s exact and Kruskal–Wallis tests (P &lt; 0.05). In all experimental periods, MTA FLPX and iRoot SP scored higher than the other groups for the variable macrophages (P &lt; 0.05). After 30- and 90-day experimental periods, MTA FLPX scored higher than the other groups for the variable MNGC (P &lt; 0.05). After 90 days, the only group that exhibited samples with severe inflammatory response was MTA FLPX. VK positivity was observed in areas of necrosis in all groups, except in the control group. The materials were considered biologically acceptable except MTA FLPX, which remained toxic to subcutaneous tissue even after 90 days.
Felippe WT, Felippe MCS, Rocha MJC. The effect of mineral trioxide aggregate on the apexification... more Felippe WT, Felippe MCS, Rocha MJC. The effect of mineral trioxide aggregate on the apexification and periapical healing of teeth with incomplete root formation. International Endodontic Journal, 39, 2-9, 2006.
dos Santos LGP, Felippe WT, Teixeira CS, Bortoluzzi EA, Felippe MCS. Endodontic re-instrumentatio... more dos Santos LGP, Felippe WT, Teixeira CS, Bortoluzzi EA, Felippe MCS. Endodontic re-instrumentation enhances hydroxyl ion diffusion through radicular dentine. International Endodontic Journal, 47, 776-783, 2014. Aim To evaluate the diffusion of hydroxyl ions from calcium hydroxide paste (CH) before root canal filling and after retreatment. Methodology After preparation of 60 root canals, the cementum layer was removed, and the canals and root surfaces were treated for smear layer removal. The apical third of roots was covered with adhesive. The canals were filled with CH, and the teeth were placed in individual vials containing 10 mL of distilled water, which had its pH measured after 7, 14, 21 and 28 days (pH1). The root canals were then divided into five groups and filled with Resilon/Real Seal (G1) or gutta-percha and Endofill (G2), Sealapex (G3), AH Plus (G4) or MTA Fillapex (G5) sealers. After storage for 7 days, the root canals were retreated. The CH was again inserted into the canals, and the teeth were placed in new vials containing 10 mL of distilled water. The pH was again measured at 7, 14, 21 and 28 days (pH2). The initial and final pH readings (pH1 and pH2) were compared by ANOVA, ANOVA2 and Tukey's tests (P < 0.05). Results The pH1 and pH2 measurements increased with time. The measurements obtained after retreatment were significantly higher than those obtained before root canal filling. Conclusions Hydroxyl ions are able to diffuse through dentinal tubules. Regardless of the filling material, it was possible to re-establish the permeability of dentine to ionic diffusion after retreatment. Time had a positive influence on ionic diffusion.
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Papers by Moises Felippe