Sažetak Uvod. Poroznost i površinska adherentnost akrilata čine ih kolektorima infektivnog sadrža... more Sažetak Uvod. Poroznost i površinska adherentnost akrilata čine ih kolektorima infektivnog sadržaja iz usne duplje. To se posebno odnosi na hladno polimerizujuće akrilate, čija je struktura manje kompaktna. Cilj rada bio je ispitivanje antimikrobnog dejstva hladno polimerizujućeg akrilata nakon njegove impregnacije nanočesticama srebra. Materijal i metode. Polimernoj komponenti (prahu) hladno polimerizovanog akrilata dodate su različite koncentracije (2%, 5% i 10%) nanočestica srebra, nakon čega su napravljeni uzorci oblika diska promera 10 mm. Kao kontrola poslužio je disk od nanočestica srebra. Antimikrobna aktivnost ispitivana je disk difuzionom metodom na dva česta izazivača infekcija usne duplje-Gram pozitivnoj bakteriji, Staphylococcus aureus ATCC 25923, i gljivici, Candidi albicans ATCC2091. Rezultati su pokazali da uzorak čistog srebra, kao i uzorci polimera sa srebrom, pokazuju antibakterijsku aktivnost. Zona inhibicije rasta Staphylococcus aureusa na hranjivoj podlozi upravno je srazmerna koncentraciji nanočestica srebra u akrilatu. Sa druge strane, ispitivani uzorci nisu ihhibirali rast Candide albicans na hranjivoj podlozi. Zaključak. Nanočestice srebra u akrilatu pokazale su antibakterijsku aktivnost. Proširenje njihovog spektra delovanja, kao i mogućnost eventualne kliničke primene biće predmet budućih istraživanja.
S U M MA R Y Among many factors influencing human beauty, the correct position of the upper front... more S U M MA R Y Among many factors influencing human beauty, the correct position of the upper front teeth, including central incisors, is undoubtedly of particular importance. When determining the intermaxillary relationship, an important phase is determination of the medial line of a person's face or places of contact of the upper central incisors. The aim of this study was to test the hypothesis that the place of mutual contact of the upper central incisors in most cases coincided with the central groove of the lower lip. Examination included two groups of respondents. The first group consisted of 100 female and the second of 100 male respondents, with age range from 18 to 30 years. A starting point in measuring was the central groove of the lower lip. Measuring the horizontal distance of the central groove of the lower lip and the mutual contact of the upper central incisors was performed with a slight touch of the upper teeth with the lower lip. The measurement was performed using a digital vernier SH, with the resolution of 0.01 mm. After performing statistical analysis, the results showed that in a large percentage of cases, the central groove of the lower lip coincided with the point of mutual contact of the upper central incisors. The point of contact of the upper incisors can be determined simply by transferring the position of the groove to the upper interocclusal rim,with a slight contact between the lips and interocclusal rim.
Sažetak Uvod: Da bi se materijal smatrao biološki prihvatljivim neophodno je da poseduje takav po... more Sažetak Uvod: Da bi se materijal smatrao biološki prihvatljivim neophodno je da poseduje takav površinski dizajn da što manje reaguje sa tkivom i agensima iz okoline.Nijedna metoda obrade ne može da proizvede molekularno ravnu površinu stomatoloških materijala. Cilj: istraživanja bio je ispitati hrapavost različitih stomatoloških materijala, pomoću mehaničkog profilometra. Materijal i metode: Ispitivani materijal obuhvatio je kompozit, toplo polimerizovani akrilat, hladno polimerizovane akrilate koji se koriste u protetici i ortodonciji, cirkonijum oksidnu keramiku i staklokermiku. Uzorci materijala za istraživanje su napravljeni prema uputstvu proizvođača. Merenje hrapavosti dobijenih uzoraka izvršeno je pomoću Mitutoyo SJ-301 Suftest uređaja, prevlačenjem čitača preko uzoraka, u dva pravca (vertikalno i horizontalno), čime su dobijena dve vrednosti merenja za svaki materijal pojedinačno. Rezultati: Merenjem hrapavosti materijala, utvrđeno je da među ispitivanim uzorcima postoje značajne razlike. Najveća hrapavost izmerena je kod hladno polimerizovanog akrilata koji se koristi u protetici, dok je najmanja hrapavost izmerenakod kompozitnog materijala. Zaključak: Hrapavost je bila značajno veća kod hladno polimerizovanih akrilata u odnosu na ostale ispitivane materijale, te ga, kada je god to moguće, treba zameniti toplo polimerizvanim akrilatom.U cilju smanjenja hrapavosti stomatoloških materijala treba poštovati principe njihove pripreme i posebno površinske obrade (postupak poliranja i glaziranja).
Sažetak Uvod: Kvalitet života u vezi sa oralnim zdravljem je važna mera bolesti i samih ishoda te... more Sažetak Uvod: Kvalitet života u vezi sa oralnim zdravljem je važna mera bolesti i samih ishoda terapijskih intervencija. Parodontopatija je inflamatorno oboljenje koje je povezano sa opštim stanjem pacijenta i negativno utiče na kvalitet života. Cilj ovog istraživanja bio je da se ispita efekat bazične terapije parodontopatije na kvalitet života kod pacijenata starijeg doba sa hroničnom parodontopatijom. Materijali i metode: Pacijenti sa parodontopatijom nasumice su raspoređeni u studijsku grupu (n=44) kod kojih je sprovedena bazična terapija,i kontrolnu grupu (n=44), koja nije primila nikakav tretman. Protokol je uključivao popunjavanje upitnika OHIP-14, pre i mesec dana posle tretmana, klinički pregled i bazičnu terapiju parodontopatije. Pacijenti su popunili i dodatna pitanja o zdravlju gingive kao i uticaju parodontopatije na kvalitet života. Rezultati: Rezultati kliničkih parodontalnih parametara nisu pokazali signifikantnu razliku među grupama na početku ispitivanja. Na kontroli je zabeleženo poboljšanje svih kliničkih parametara i signifikantna razlika između ispitivanih grupa (p<0,05), kao i u tretiranoj grupi pre i posle tretmana (p<0,001). Statistički značajne promene (p<0,001) u tretiranoj grupi zabeležene su za impakciju hrane, izbegavanje unosa određene vrste hrane, loš zadah, izbegavanje smejanja i prisustvo apscesa. Zdravlje gingive ocenjeno je sa "loše" ili "veoma loše" kod 42,73% ispitanika. Uticaj parodontopatije na svakodnevne aktivnosti kod pacijenata izražen je sa "često" ili "uvek" kada se smatralo da imaju najčešće probleme vezane za ove aktivnosti . Zaključak: Bazična tarapija parodontopatije značajno je uticala na kvalitet života pacijenata starijeg doba sa hroničnom parodontopatijom. Utvrđene su značajne razlike između odgovora pre i posle tretmana. U ovoj studiji uočen je prihvaćeni stepen svesti o problemima oralnog zdravlja u vezi sa parodontalnom bolešću kod pacijenata starijeg doba.
Uvod: Bruksizam je ponavljana aktivnost mišića i zglobova koja se karakteriše škripanjem i struga... more Uvod: Bruksizam je ponavljana aktivnost mišića i zglobova koja se karakteriše škripanjem i struganjem zubima, a klinički se, pre svega, može dijagnostikovati prisustvom izraženih brusnih faseta. Cilj rada bio je prikaz pacijenta sa bruksizmom kome je usled dijagnostikovane krezubosti indikovana izrada gornjeg i donjeg polucirkularnog metalo-keramičkog mosta. Kliničkim pregledom utvrđeno je značajno oštećenje prethodno izrađenih fiksih nadoknada. Povećanjem vertikalne dimenzije okluzije, stabilnim okluzalnim kontaktima metalo-keramičkih članova i izradom intraoralnih splintova od meke plastike postignuti su optimalni terapijski rezultati, a oštećenja mosta su izostala. Zaključak: Izrada protetskih radova kod pacijenata sa bruksizmom ima za cilj da primarno reši nedostatak izgubljenih zuba, ali i preventivno deluje na moguća oštećenja orofacijalnog sistema parafunkcijskim aktiv-nostima.
Sažetak Uvod: Meki akrilati pripadaju grupi hladno polimerizovanih materijala koji se aplikuju na... more Sažetak Uvod: Meki akrilati pripadaju grupi hladno polimerizovanih materijala koji se aplikuju na bazalnu površinu zubne proteze kako bi se eliminisale mehaničke iritacije, omogućilo ozdravljenje oštećene ili inflamirane sluzokože ili se oralno tkivo pripremilo (kondicioniralo) za prihvatanje nove nadoknade. Cilj istraživanja bio je ispitivanje ritma oslobađanja komponenti mekih akrilata u tri različita modela veštačke pljuvačke, u toku tridesetodnevnog opservacionog perioda. Materijal i metode: U ispitivanju su korišćena dva meka akrilata:poli (etil metakrilat)/ n-butil metakrilat i poli (etil metakrilat) / metil metakrilat, koji su odlagani u tri različita modela veštačke pljuvačke, u okviru tri opservaciona perioda: jedan, sedam i trideset dana. Ispitivanje je obuhvatilo detekciju metil metakrilata, etil metakrilata, butil metakrilata, di butil ftalata i benzoil peroksida tečnom hromatografijom pod visokim pritiskom. Rezultati: Količina oslobođenih komponenti srazmerno se povećava sa porastom trajanja opservacionog perioda, bez obzira na model veštačke pljuvačke.Najviše vrednosti svih ispitivanih parametara uočene su nakon tridesetodnevnog opservacionog perioda. Model veštačke pljuvačke nije uticao na ritam oslobađanja komponenti, što znači da se one nesmetano oslobađaju u usnu duplju bez obzira na sastav pljuvačke pacijenta. Zaključak: Sa porastom dužine opservacionog perioda došlo je i do očvršavanja materijala, čime se završava njihova upotrebna vrednost. Abstract Introduction: Soft acrylic resins belong to a group of cold curing materials that are applied to the basal surface of the dental prosthesis in order to eliminate mechanical irritation, and to allow for the recovery of damaged or inflamed mucous membranes, and prepare the oral tissue to accept a new compensation. The aim of the study was to examine the rhythm of the release of soft acrylic resin components in three different models of artificial saliva, during a thirty-day observation period. Material and Methods. Two soft acrylic resins were used in the study: poly (ethyl methacrylate)/n-butyl methacrylate and poly (ethyl methacrylate)/methyl methacrylate, which were deposited in three different models of artificial saliva within the three observation periods: one, seven and thirty days. The test involved the detection of methyl methacrylate, ethyl methacrylate, butyl methacrylate, dibutyl phthalate and benzoyl peroxide under high-pressure liquid chromatography. Results: The amount of released components increased proportionately with the increase in the duration of the observation period, regardless of the artificial saliva model. The highest values of all tested parameters were detected after a thirty-day observation period. The artificial saliva model did not affect the release of the components, which means they are freely released into the oral cavity regardless of the saliva composition of the patient. Conclusion:As the length of the observation period increased, the material was solidified, thus ending their use value.
The aim of the present study was to determine the content of residual monomer methyl methacrylate... more The aim of the present study was to determine the content of residual monomer methyl methacrylate (MMA) in cold and hot polymerized poly(methyl methacrylate) (PMMA), both widely applicable in dentistry, by using MHE-GC-MS (multiple headspace extraction analysis by gas chromatographymass spectrometry) in combination with GC-FID (gas chromatography with flame-ionization detection). The samples of PMMA were obtained by free-radical polymerization of MMA at room temperature (cold polymerized PMMA) and at 100 C (hot polymerized PMMA), according to the manufacturer's instructions. The MHE-GC-MS method consisted of ten successive extractions of MMA from the polymerized samples. According to the constructed calibration curve, the content of residual MMA in PMMA samples (mg MMA /g PMMA ) was determined. Intramuscular implantation of sample materials was performed in experimental animals (rats) in order to demonstrate potential adverse effects of the unpolymerized residual reactants to the tissue in direct contact with polymeric implants. The content of residual monomer in cold polymerized PMMA was higher (15.75 mg MMA/ g PMMA ) in comparison to hot polymerized PMMA (10.96 mg MMA/ g PMMA ). The implanted samples of cold-polymerized acrylic resin showed intense inflammatory response of the surrounding tissue. According to the obtained results, hot polymerized PMMA is safer for use in dentistry.
Restoring the morpho-functional capacity of destroyed and compensations of lost teeth present a c... more Restoring the morpho-functional capacity of destroyed and compensations of lost teeth present a challenge for the synthesis of new more effective materials and the improvement of those already on the market. The aim of the manuscript was to present the advantages and disadvantages of contemporary acrylic polymers, dental ceramics, and composites, based on literature data and published research results. The choice of the adequate material is crucial for making not only dental but any product. The synthesis of new materials and the development of new technological procedures have accelerated the advancement of dentistry. Therefore, today's dental compensations faithfully imitate natural teeth and can fully perform their intended function.
Introduction:Dental materials play a role of morphological and functional substituent of the oral... more Introduction:Dental materials play a role of morphological and functional substituent of the oral environment and they are expected to perform their function without causing adverse effects. A biofilm is formed on the surface of dental materials, as well as on other oral tissues. Considering that dental materials due to their structure, in most cases, allow accumulation of food residues and infectious content, a comparative review of possible consequences and the way of their prevention is of great importance. The aim of this manuscript was the analysis of biofilm formation on different dental materials surfaces based on published investigations and literature data. Knowing the structure of dental materials and their behavior in oral environment is a base for proper setting of indication for their use. The simplest way to control biofilm formation on materials is good oral hygiene and maintaining dentures. Conclusion:The formation of biofilm in dental materials lead to development of same deseases of oral cavity.The simplest way to control the development of biofilm is to maintain a ligh lovel of oral higiene.
Acta Stomatologica Naissi Decembar/December 2010, vol. 26, br./number 62 str./p 1007 -1016 KRATAK... more Acta Stomatologica Naissi Decembar/December 2010, vol. 26, br./number 62 str./p 1007 -1016 KRATAK SADRŽAJ Uvod.Biokompatibilnost, kao esencijalna osobina materijala u stomatologiji, podrazumeva sposobnost materijala da nakon aplikacije obavlja određenu funkciju u organizmu ne izazivajući neželjeni odgovor tkiva domaćina. Testovi za ispitivanje biokompatibilnosti služe za detekciju sastojaka materijala koji bi mogli da dovedu do povrede ili oštećenja tkiva usne duplje i organizma uopšte. Cilj rada bio je opis metoda koje se koriste za ispitivanje biokompatibilnosti materijala u stomatologiji. Materijal i metode. Urađena je analiza publikovanih radova u periodu od 1990-2009. godine, i koji su indeksirana u Medline bazi podataka (US National Library of Medicine). Korišćene su ključne reči: testiranje, biokompatibilnost, stomatološki materijali. Rezultati. U opisanom periodu na temu ispitivanja biokompatibilnosti različitih stomatoloških materijala objavljeno je 505 radova od kojih je 53 preglednih radova. 194 radova odnosilo se na in vitro, a 101 rad na in vivo istraživanja biokompatibilnosti. Zaključak. Savremeni aspekt ispitivanja biokompatibilnosti stomatoloških materijala podrazumeva tri nivoa istraživanja: in vitro u laboratorijskim uslovima, in vivo testove na eksperimentalnim životinjama i testove primene. Klinička relevantnost rezultata dobijenih u uslovima in vitro je diskutabilna, ali se njima može znatno smanjiti broj testova na životinjama. Prava ocena o nekom materijalu može dati tek nakon dužeg korišćenja u kliničkoj praksi. Ključne reči: testiranje, biokompatibilnost, stomatološki materijali
Kostić i sar. / Promene na hela ćelijskoj kulturi u prisustvu akrilata za bazu proteze KliniKa za... more Kostić i sar. / Promene na hela ćelijskoj kulturi u prisustvu akrilata za bazu proteze KliniKa za stomatologiju, odeljenje za stomatološKu ProtetiKu, niš, srbija; 2 medicinsKi faKultet, niš, srbija; 3 institut za biologiju i humanu genetiKu, niš, srbija 1 clinic of stomatology, dePartment of Prosthodontics, nis, serbia; 2 medical faculty, nis, serbia; 3 institut of biomedical research, nis, serbia acta stomatologica naissi decembar/december 2008, vol. 24, br./num. 58 str./p 793-800 Kratak sadržaj Akrilati su često upotrebljavani materijali u stomatoprotetskoj praksi, posebno u izradi baza zubnih proteza. U pojedinim slučajevima uočava se reakcija oralnih tkiva na mestima kontakta sa bazom akrilatnih proteza. Materijal za ispitivanje je obuhvatao četiri različita akrilata za bazu proteze. Ispitivan je uticaj ekstrakata akrilata različitih koncentracija na vijabilnost HeLa ćelija, kao i reverzibilnost nastalih promena na ćelijskoj kulturi. Kao kontrola je služila kultura koja je rasla u medijumu bez ekstrakata. Procena vijabilnosti HeLa ćelija vršena je MTT testom. Sa porastom koncentracije ekstrakata ispitivanih akrilata vijabilnost HeLa ćelija značajno opada, a i usporeniji je njihov oporavak. Potpuni oporavak HeLa ćelija nije primećen ni u jednoj od ispitivanih koncentracija. Ključne reči: akrilati, baza proteze, HeLa ćelijska kultura, MTT test
Background/Aim. Acrylic-based resins are intensively used in dentistry practice as restorative or... more Background/Aim. Acrylic-based resins are intensively used in dentistry practice as restorative or denture-base materials. The purpose of this study was to analyze the surface structure of denture base resins and the amount of released potentially toxic substances (PTS) immediately upon polymerization and incubation in different types of artificial saliva. Methods. Storage of acrylic samples in two models of artificial saliva were performed in a water bath at the temperature of 37 ± 1°C. Analysis of the surface structure of samples was carried out using scanning electronic microscopy analysis immediately after polymerization and after the 30-day incubation. The amounts of PTS per day, week and month extracts were measured using high-pressure liquid chromatography. Results. Surface design and amount of PTS in acrylic materials were different and depended on the types and duration of polymerization. The surfaces of tested acrylates became flatter after immersing in solutions of artificial saliva. The degree of acrylic materials release was not dependent on the applied model of artificial saliva. Conclusion. In order to improve biological features of acrylic resin materials, it was recommended that dentures lined with soft or hard coldpolymerized acrylates should be kept at least 1 to 7 days in water before being given to a patient. So, as to reach high degree of biocompatibility preparation of prosthetic restorations from heat-polymerized acrylate was unnecessary.
Backround/Aim. Retraction agents for temporary vertical and lateral suppression of gingival tissu... more Backround/Aim. Retraction agents for temporary vertical and lateral suppression of gingival tissue as well as bleeding control and fluid flow in the gingival sulcus are expected to have maximal efficiency without irreversible damage of local tissue and adverse systemic effects. The research started from the assumption that tetrahydrozoline is a biologically more acceptable means of gingival retraction than commercially available preparations. The aim of the study was to comparatively analyse the inflammatory effects of different retraction materials and tetrahydrozoline. Methods. The effect of retraction liquid on the basis of aluminum chloride and epinephrine and tetrahydrozoline hydrochloride on gingival tissue of rabbits was investigated. The application time in the rabbit's gingival sulcus was 7 minutes. Tissue biopsy was performed after an hour, a day, and 7 and 30 days. Tissue preparations were analyzed under a microscope. Results. The obtained results indicate a reversible damage of gingival tissues as a result of local application of aluminum chloride-and epinephrine-based retraction agents. Their use led to acute inflammatory response after an observation period of 1 and 7 days. After 30 days reparation of damaged tissue was observed. The use of tetrahydrozoline resulted in a visibly weaker inflammatory response. Conclusion. Retraction liquids insertion led to an acute inflammatory response of gingival tissue which in time assumed a chronic character. The inflammatory response to the administered tetrahydrozoline was significantly lower with complete reparation of gingival tissue. Taking this fact into account it is recommended as a potential retraction agent.
The use of acrylic denture base resins is widely spread in dental practice. They belong to the gr... more The use of acrylic denture base resins is widely spread in dental practice. They belong to the group of biomaterials due to their role of morphological and functional substituent in the mouth. However, clinical practice has shown that some toxic ingredients of these materials may lead to adverse local and even systemic changes.
The alveolar bone tissue resorption defect has a significant role in dentistry. Because of the bo... more The alveolar bone tissue resorption defect has a significant role in dentistry. Because of the bone tissue deficit developed by alveolar resorption, the use of synthetic material CP/PLGA (calcium-phosphate/polylactide-co-gliycolide) composite was introduced. Investigations were performed on rats with artificially produced resorption of the mandibular bone. The results show that the best effect on alveolar bone were attained by using nano-composite implants. The effect of the nanocomposite was ascertained by determining the calcium and phosphate content, as a basis of the hydroxyapatite structure. The results show that synthetic CP/PLGA nanocomposite alleviate the rehabilitation of weakened alveolar bone. Due to its osteoconductive effect, CP/PLGA can be the material of choice for bone substitution in the future.
Sažetak Uvod. Poroznost i površinska adherentnost akrilata čine ih kolektorima infektivnog sadrža... more Sažetak Uvod. Poroznost i površinska adherentnost akrilata čine ih kolektorima infektivnog sadržaja iz usne duplje. To se posebno odnosi na hladno polimerizujuće akrilate, čija je struktura manje kompaktna. Cilj rada bio je ispitivanje antimikrobnog dejstva hladno polimerizujućeg akrilata nakon njegove impregnacije nanočesticama srebra. Materijal i metode. Polimernoj komponenti (prahu) hladno polimerizovanog akrilata dodate su različite koncentracije (2%, 5% i 10%) nanočestica srebra, nakon čega su napravljeni uzorci oblika diska promera 10 mm. Kao kontrola poslužio je disk od nanočestica srebra. Antimikrobna aktivnost ispitivana je disk difuzionom metodom na dva česta izazivača infekcija usne duplje-Gram pozitivnoj bakteriji, Staphylococcus aureus ATCC 25923, i gljivici, Candidi albicans ATCC2091. Rezultati su pokazali da uzorak čistog srebra, kao i uzorci polimera sa srebrom, pokazuju antibakterijsku aktivnost. Zona inhibicije rasta Staphylococcus aureusa na hranjivoj podlozi upravno je srazmerna koncentraciji nanočestica srebra u akrilatu. Sa druge strane, ispitivani uzorci nisu ihhibirali rast Candide albicans na hranjivoj podlozi. Zaključak. Nanočestice srebra u akrilatu pokazale su antibakterijsku aktivnost. Proširenje njihovog spektra delovanja, kao i mogućnost eventualne kliničke primene biće predmet budućih istraživanja.
S U M MA R Y Among many factors influencing human beauty, the correct position of the upper front... more S U M MA R Y Among many factors influencing human beauty, the correct position of the upper front teeth, including central incisors, is undoubtedly of particular importance. When determining the intermaxillary relationship, an important phase is determination of the medial line of a person's face or places of contact of the upper central incisors. The aim of this study was to test the hypothesis that the place of mutual contact of the upper central incisors in most cases coincided with the central groove of the lower lip. Examination included two groups of respondents. The first group consisted of 100 female and the second of 100 male respondents, with age range from 18 to 30 years. A starting point in measuring was the central groove of the lower lip. Measuring the horizontal distance of the central groove of the lower lip and the mutual contact of the upper central incisors was performed with a slight touch of the upper teeth with the lower lip. The measurement was performed using a digital vernier SH, with the resolution of 0.01 mm. After performing statistical analysis, the results showed that in a large percentage of cases, the central groove of the lower lip coincided with the point of mutual contact of the upper central incisors. The point of contact of the upper incisors can be determined simply by transferring the position of the groove to the upper interocclusal rim,with a slight contact between the lips and interocclusal rim.
Sažetak Uvod: Da bi se materijal smatrao biološki prihvatljivim neophodno je da poseduje takav po... more Sažetak Uvod: Da bi se materijal smatrao biološki prihvatljivim neophodno je da poseduje takav površinski dizajn da što manje reaguje sa tkivom i agensima iz okoline.Nijedna metoda obrade ne može da proizvede molekularno ravnu površinu stomatoloških materijala. Cilj: istraživanja bio je ispitati hrapavost različitih stomatoloških materijala, pomoću mehaničkog profilometra. Materijal i metode: Ispitivani materijal obuhvatio je kompozit, toplo polimerizovani akrilat, hladno polimerizovane akrilate koji se koriste u protetici i ortodonciji, cirkonijum oksidnu keramiku i staklokermiku. Uzorci materijala za istraživanje su napravljeni prema uputstvu proizvođača. Merenje hrapavosti dobijenih uzoraka izvršeno je pomoću Mitutoyo SJ-301 Suftest uređaja, prevlačenjem čitača preko uzoraka, u dva pravca (vertikalno i horizontalno), čime su dobijena dve vrednosti merenja za svaki materijal pojedinačno. Rezultati: Merenjem hrapavosti materijala, utvrđeno je da među ispitivanim uzorcima postoje značajne razlike. Najveća hrapavost izmerena je kod hladno polimerizovanog akrilata koji se koristi u protetici, dok je najmanja hrapavost izmerenakod kompozitnog materijala. Zaključak: Hrapavost je bila značajno veća kod hladno polimerizovanih akrilata u odnosu na ostale ispitivane materijale, te ga, kada je god to moguće, treba zameniti toplo polimerizvanim akrilatom.U cilju smanjenja hrapavosti stomatoloških materijala treba poštovati principe njihove pripreme i posebno površinske obrade (postupak poliranja i glaziranja).
Sažetak Uvod: Kvalitet života u vezi sa oralnim zdravljem je važna mera bolesti i samih ishoda te... more Sažetak Uvod: Kvalitet života u vezi sa oralnim zdravljem je važna mera bolesti i samih ishoda terapijskih intervencija. Parodontopatija je inflamatorno oboljenje koje je povezano sa opštim stanjem pacijenta i negativno utiče na kvalitet života. Cilj ovog istraživanja bio je da se ispita efekat bazične terapije parodontopatije na kvalitet života kod pacijenata starijeg doba sa hroničnom parodontopatijom. Materijali i metode: Pacijenti sa parodontopatijom nasumice su raspoređeni u studijsku grupu (n=44) kod kojih je sprovedena bazična terapija,i kontrolnu grupu (n=44), koja nije primila nikakav tretman. Protokol je uključivao popunjavanje upitnika OHIP-14, pre i mesec dana posle tretmana, klinički pregled i bazičnu terapiju parodontopatije. Pacijenti su popunili i dodatna pitanja o zdravlju gingive kao i uticaju parodontopatije na kvalitet života. Rezultati: Rezultati kliničkih parodontalnih parametara nisu pokazali signifikantnu razliku među grupama na početku ispitivanja. Na kontroli je zabeleženo poboljšanje svih kliničkih parametara i signifikantna razlika između ispitivanih grupa (p<0,05), kao i u tretiranoj grupi pre i posle tretmana (p<0,001). Statistički značajne promene (p<0,001) u tretiranoj grupi zabeležene su za impakciju hrane, izbegavanje unosa određene vrste hrane, loš zadah, izbegavanje smejanja i prisustvo apscesa. Zdravlje gingive ocenjeno je sa "loše" ili "veoma loše" kod 42,73% ispitanika. Uticaj parodontopatije na svakodnevne aktivnosti kod pacijenata izražen je sa "često" ili "uvek" kada se smatralo da imaju najčešće probleme vezane za ove aktivnosti . Zaključak: Bazična tarapija parodontopatije značajno je uticala na kvalitet života pacijenata starijeg doba sa hroničnom parodontopatijom. Utvrđene su značajne razlike između odgovora pre i posle tretmana. U ovoj studiji uočen je prihvaćeni stepen svesti o problemima oralnog zdravlja u vezi sa parodontalnom bolešću kod pacijenata starijeg doba.
Uvod: Bruksizam je ponavljana aktivnost mišića i zglobova koja se karakteriše škripanjem i struga... more Uvod: Bruksizam je ponavljana aktivnost mišića i zglobova koja se karakteriše škripanjem i struganjem zubima, a klinički se, pre svega, može dijagnostikovati prisustvom izraženih brusnih faseta. Cilj rada bio je prikaz pacijenta sa bruksizmom kome je usled dijagnostikovane krezubosti indikovana izrada gornjeg i donjeg polucirkularnog metalo-keramičkog mosta. Kliničkim pregledom utvrđeno je značajno oštećenje prethodno izrađenih fiksih nadoknada. Povećanjem vertikalne dimenzije okluzije, stabilnim okluzalnim kontaktima metalo-keramičkih članova i izradom intraoralnih splintova od meke plastike postignuti su optimalni terapijski rezultati, a oštećenja mosta su izostala. Zaključak: Izrada protetskih radova kod pacijenata sa bruksizmom ima za cilj da primarno reši nedostatak izgubljenih zuba, ali i preventivno deluje na moguća oštećenja orofacijalnog sistema parafunkcijskim aktiv-nostima.
Sažetak Uvod: Meki akrilati pripadaju grupi hladno polimerizovanih materijala koji se aplikuju na... more Sažetak Uvod: Meki akrilati pripadaju grupi hladno polimerizovanih materijala koji se aplikuju na bazalnu površinu zubne proteze kako bi se eliminisale mehaničke iritacije, omogućilo ozdravljenje oštećene ili inflamirane sluzokože ili se oralno tkivo pripremilo (kondicioniralo) za prihvatanje nove nadoknade. Cilj istraživanja bio je ispitivanje ritma oslobađanja komponenti mekih akrilata u tri različita modela veštačke pljuvačke, u toku tridesetodnevnog opservacionog perioda. Materijal i metode: U ispitivanju su korišćena dva meka akrilata:poli (etil metakrilat)/ n-butil metakrilat i poli (etil metakrilat) / metil metakrilat, koji su odlagani u tri različita modela veštačke pljuvačke, u okviru tri opservaciona perioda: jedan, sedam i trideset dana. Ispitivanje je obuhvatilo detekciju metil metakrilata, etil metakrilata, butil metakrilata, di butil ftalata i benzoil peroksida tečnom hromatografijom pod visokim pritiskom. Rezultati: Količina oslobođenih komponenti srazmerno se povećava sa porastom trajanja opservacionog perioda, bez obzira na model veštačke pljuvačke.Najviše vrednosti svih ispitivanih parametara uočene su nakon tridesetodnevnog opservacionog perioda. Model veštačke pljuvačke nije uticao na ritam oslobađanja komponenti, što znači da se one nesmetano oslobađaju u usnu duplju bez obzira na sastav pljuvačke pacijenta. Zaključak: Sa porastom dužine opservacionog perioda došlo je i do očvršavanja materijala, čime se završava njihova upotrebna vrednost. Abstract Introduction: Soft acrylic resins belong to a group of cold curing materials that are applied to the basal surface of the dental prosthesis in order to eliminate mechanical irritation, and to allow for the recovery of damaged or inflamed mucous membranes, and prepare the oral tissue to accept a new compensation. The aim of the study was to examine the rhythm of the release of soft acrylic resin components in three different models of artificial saliva, during a thirty-day observation period. Material and Methods. Two soft acrylic resins were used in the study: poly (ethyl methacrylate)/n-butyl methacrylate and poly (ethyl methacrylate)/methyl methacrylate, which were deposited in three different models of artificial saliva within the three observation periods: one, seven and thirty days. The test involved the detection of methyl methacrylate, ethyl methacrylate, butyl methacrylate, dibutyl phthalate and benzoyl peroxide under high-pressure liquid chromatography. Results: The amount of released components increased proportionately with the increase in the duration of the observation period, regardless of the artificial saliva model. The highest values of all tested parameters were detected after a thirty-day observation period. The artificial saliva model did not affect the release of the components, which means they are freely released into the oral cavity regardless of the saliva composition of the patient. Conclusion:As the length of the observation period increased, the material was solidified, thus ending their use value.
The aim of the present study was to determine the content of residual monomer methyl methacrylate... more The aim of the present study was to determine the content of residual monomer methyl methacrylate (MMA) in cold and hot polymerized poly(methyl methacrylate) (PMMA), both widely applicable in dentistry, by using MHE-GC-MS (multiple headspace extraction analysis by gas chromatographymass spectrometry) in combination with GC-FID (gas chromatography with flame-ionization detection). The samples of PMMA were obtained by free-radical polymerization of MMA at room temperature (cold polymerized PMMA) and at 100 C (hot polymerized PMMA), according to the manufacturer's instructions. The MHE-GC-MS method consisted of ten successive extractions of MMA from the polymerized samples. According to the constructed calibration curve, the content of residual MMA in PMMA samples (mg MMA /g PMMA ) was determined. Intramuscular implantation of sample materials was performed in experimental animals (rats) in order to demonstrate potential adverse effects of the unpolymerized residual reactants to the tissue in direct contact with polymeric implants. The content of residual monomer in cold polymerized PMMA was higher (15.75 mg MMA/ g PMMA ) in comparison to hot polymerized PMMA (10.96 mg MMA/ g PMMA ). The implanted samples of cold-polymerized acrylic resin showed intense inflammatory response of the surrounding tissue. According to the obtained results, hot polymerized PMMA is safer for use in dentistry.
Restoring the morpho-functional capacity of destroyed and compensations of lost teeth present a c... more Restoring the morpho-functional capacity of destroyed and compensations of lost teeth present a challenge for the synthesis of new more effective materials and the improvement of those already on the market. The aim of the manuscript was to present the advantages and disadvantages of contemporary acrylic polymers, dental ceramics, and composites, based on literature data and published research results. The choice of the adequate material is crucial for making not only dental but any product. The synthesis of new materials and the development of new technological procedures have accelerated the advancement of dentistry. Therefore, today's dental compensations faithfully imitate natural teeth and can fully perform their intended function.
Introduction:Dental materials play a role of morphological and functional substituent of the oral... more Introduction:Dental materials play a role of morphological and functional substituent of the oral environment and they are expected to perform their function without causing adverse effects. A biofilm is formed on the surface of dental materials, as well as on other oral tissues. Considering that dental materials due to their structure, in most cases, allow accumulation of food residues and infectious content, a comparative review of possible consequences and the way of their prevention is of great importance. The aim of this manuscript was the analysis of biofilm formation on different dental materials surfaces based on published investigations and literature data. Knowing the structure of dental materials and their behavior in oral environment is a base for proper setting of indication for their use. The simplest way to control biofilm formation on materials is good oral hygiene and maintaining dentures. Conclusion:The formation of biofilm in dental materials lead to development of same deseases of oral cavity.The simplest way to control the development of biofilm is to maintain a ligh lovel of oral higiene.
Acta Stomatologica Naissi Decembar/December 2010, vol. 26, br./number 62 str./p 1007 -1016 KRATAK... more Acta Stomatologica Naissi Decembar/December 2010, vol. 26, br./number 62 str./p 1007 -1016 KRATAK SADRŽAJ Uvod.Biokompatibilnost, kao esencijalna osobina materijala u stomatologiji, podrazumeva sposobnost materijala da nakon aplikacije obavlja određenu funkciju u organizmu ne izazivajući neželjeni odgovor tkiva domaćina. Testovi za ispitivanje biokompatibilnosti služe za detekciju sastojaka materijala koji bi mogli da dovedu do povrede ili oštećenja tkiva usne duplje i organizma uopšte. Cilj rada bio je opis metoda koje se koriste za ispitivanje biokompatibilnosti materijala u stomatologiji. Materijal i metode. Urađena je analiza publikovanih radova u periodu od 1990-2009. godine, i koji su indeksirana u Medline bazi podataka (US National Library of Medicine). Korišćene su ključne reči: testiranje, biokompatibilnost, stomatološki materijali. Rezultati. U opisanom periodu na temu ispitivanja biokompatibilnosti različitih stomatoloških materijala objavljeno je 505 radova od kojih je 53 preglednih radova. 194 radova odnosilo se na in vitro, a 101 rad na in vivo istraživanja biokompatibilnosti. Zaključak. Savremeni aspekt ispitivanja biokompatibilnosti stomatoloških materijala podrazumeva tri nivoa istraživanja: in vitro u laboratorijskim uslovima, in vivo testove na eksperimentalnim životinjama i testove primene. Klinička relevantnost rezultata dobijenih u uslovima in vitro je diskutabilna, ali se njima može znatno smanjiti broj testova na životinjama. Prava ocena o nekom materijalu može dati tek nakon dužeg korišćenja u kliničkoj praksi. Ključne reči: testiranje, biokompatibilnost, stomatološki materijali
Kostić i sar. / Promene na hela ćelijskoj kulturi u prisustvu akrilata za bazu proteze KliniKa za... more Kostić i sar. / Promene na hela ćelijskoj kulturi u prisustvu akrilata za bazu proteze KliniKa za stomatologiju, odeljenje za stomatološKu ProtetiKu, niš, srbija; 2 medicinsKi faKultet, niš, srbija; 3 institut za biologiju i humanu genetiKu, niš, srbija 1 clinic of stomatology, dePartment of Prosthodontics, nis, serbia; 2 medical faculty, nis, serbia; 3 institut of biomedical research, nis, serbia acta stomatologica naissi decembar/december 2008, vol. 24, br./num. 58 str./p 793-800 Kratak sadržaj Akrilati su često upotrebljavani materijali u stomatoprotetskoj praksi, posebno u izradi baza zubnih proteza. U pojedinim slučajevima uočava se reakcija oralnih tkiva na mestima kontakta sa bazom akrilatnih proteza. Materijal za ispitivanje je obuhvatao četiri različita akrilata za bazu proteze. Ispitivan je uticaj ekstrakata akrilata različitih koncentracija na vijabilnost HeLa ćelija, kao i reverzibilnost nastalih promena na ćelijskoj kulturi. Kao kontrola je služila kultura koja je rasla u medijumu bez ekstrakata. Procena vijabilnosti HeLa ćelija vršena je MTT testom. Sa porastom koncentracije ekstrakata ispitivanih akrilata vijabilnost HeLa ćelija značajno opada, a i usporeniji je njihov oporavak. Potpuni oporavak HeLa ćelija nije primećen ni u jednoj od ispitivanih koncentracija. Ključne reči: akrilati, baza proteze, HeLa ćelijska kultura, MTT test
Background/Aim. Acrylic-based resins are intensively used in dentistry practice as restorative or... more Background/Aim. Acrylic-based resins are intensively used in dentistry practice as restorative or denture-base materials. The purpose of this study was to analyze the surface structure of denture base resins and the amount of released potentially toxic substances (PTS) immediately upon polymerization and incubation in different types of artificial saliva. Methods. Storage of acrylic samples in two models of artificial saliva were performed in a water bath at the temperature of 37 ± 1°C. Analysis of the surface structure of samples was carried out using scanning electronic microscopy analysis immediately after polymerization and after the 30-day incubation. The amounts of PTS per day, week and month extracts were measured using high-pressure liquid chromatography. Results. Surface design and amount of PTS in acrylic materials were different and depended on the types and duration of polymerization. The surfaces of tested acrylates became flatter after immersing in solutions of artificial saliva. The degree of acrylic materials release was not dependent on the applied model of artificial saliva. Conclusion. In order to improve biological features of acrylic resin materials, it was recommended that dentures lined with soft or hard coldpolymerized acrylates should be kept at least 1 to 7 days in water before being given to a patient. So, as to reach high degree of biocompatibility preparation of prosthetic restorations from heat-polymerized acrylate was unnecessary.
Backround/Aim. Retraction agents for temporary vertical and lateral suppression of gingival tissu... more Backround/Aim. Retraction agents for temporary vertical and lateral suppression of gingival tissue as well as bleeding control and fluid flow in the gingival sulcus are expected to have maximal efficiency without irreversible damage of local tissue and adverse systemic effects. The research started from the assumption that tetrahydrozoline is a biologically more acceptable means of gingival retraction than commercially available preparations. The aim of the study was to comparatively analyse the inflammatory effects of different retraction materials and tetrahydrozoline. Methods. The effect of retraction liquid on the basis of aluminum chloride and epinephrine and tetrahydrozoline hydrochloride on gingival tissue of rabbits was investigated. The application time in the rabbit's gingival sulcus was 7 minutes. Tissue biopsy was performed after an hour, a day, and 7 and 30 days. Tissue preparations were analyzed under a microscope. Results. The obtained results indicate a reversible damage of gingival tissues as a result of local application of aluminum chloride-and epinephrine-based retraction agents. Their use led to acute inflammatory response after an observation period of 1 and 7 days. After 30 days reparation of damaged tissue was observed. The use of tetrahydrozoline resulted in a visibly weaker inflammatory response. Conclusion. Retraction liquids insertion led to an acute inflammatory response of gingival tissue which in time assumed a chronic character. The inflammatory response to the administered tetrahydrozoline was significantly lower with complete reparation of gingival tissue. Taking this fact into account it is recommended as a potential retraction agent.
The use of acrylic denture base resins is widely spread in dental practice. They belong to the gr... more The use of acrylic denture base resins is widely spread in dental practice. They belong to the group of biomaterials due to their role of morphological and functional substituent in the mouth. However, clinical practice has shown that some toxic ingredients of these materials may lead to adverse local and even systemic changes.
The alveolar bone tissue resorption defect has a significant role in dentistry. Because of the bo... more The alveolar bone tissue resorption defect has a significant role in dentistry. Because of the bone tissue deficit developed by alveolar resorption, the use of synthetic material CP/PLGA (calcium-phosphate/polylactide-co-gliycolide) composite was introduced. Investigations were performed on rats with artificially produced resorption of the mandibular bone. The results show that the best effect on alveolar bone were attained by using nano-composite implants. The effect of the nanocomposite was ascertained by determining the calcium and phosphate content, as a basis of the hydroxyapatite structure. The results show that synthetic CP/PLGA nanocomposite alleviate the rehabilitation of weakened alveolar bone. Due to its osteoconductive effect, CP/PLGA can be the material of choice for bone substitution in the future.
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