Journal of Agricultural Science and Technology, 2013
This study pursued the molecular identification of fish species from processed products for human... more This study pursued the molecular identification of fish species from processed products for human consumption which, a priori, belonged to nine species. DNA barcoding using direct sequencing of about 650 bp of the mitochondrial gene cytochrome oxidase subunit I (COI) revealed incorrect labeling in the three Alaska Pollack samples (11% of all samples). Substitution of fish species constitutes serious economic fraud, and our results increase concern regarding the trading of processed fish products in Iran from both health and conservation points of view.
The aim of this study was to investigate the effects of slow and quick freezing on the changes in... more The aim of this study was to investigate the effects of slow and quick freezing on the changes in sensory properties, drip loss, microstructure and proximate compositions of Nile Tilapia (Oreochromis niloticus) fillets. For this reason, skined off and deboned tilapia fillets were frozen by slow and quick freezing methods. The samples were packed and stored at-18 °C for six months. Proximate composition, drip loss, and sensory evaluation of the samples were determined on a montly basis. Microstructure of the samples was studied using Scanning Electron Microscopy (SEM) every second month. Results indicated that fresh tilapia fillets had 1/30, 18/70, 1/85, 79/12 percentage of fat, protein, ash and moisture contents, respectively. The amounts of proximate compositions were changed during the storageperiod. Quick frozen samples had significantly lower changes than slow frozen samples. The percentage of the drip in the slow frozen samples was significantly higher than quick frozen samples. SEM micrographs were also showed that the changes in the microstructure of the samples were different in the slow and frozen samples. Slow freezing method resulted in the higher damage in the microstructure of the samples than quick freezing method. Sensory evaluation of the samples indicated a better acceptability for the quick frozen samples than that for slow frozen sample.
Lysozymes are key molecules of innateimmunity act as non-specific innate-immunity molecules again... more Lysozymes are key molecules of innateimmunity act as non-specific innate-immunity molecules against the invasion of bacteria pathogens in fish.The aim of the present study was to amplify and express a full-length g-type lysozyme cDNA from the skin mucus of common carp (Cyprinus carpio).In this respect, the complete cDNA consisted of an open reading frame of 555 bp which encoded a protein of 185 amino acids. Regardingly, conserved and catalytic residues of this cDNA were identified which showed some similarity with Danio rerio , Salmo salar , Gallus gallus , Xenopus laevisand Tetradon nigroviridis. This Recombinant g-type lysozyme cDNA has been cloned into pMALc2x and expressed in E. colistrain TG1. The resulted recombinant protein showed lytic activity against Micrococcus lysodeikticus.
Background: The recent technological advancement has regrettably resulted in the preference of pa... more Background: The recent technological advancement has regrettably resulted in the preference of para clinical studies over physical examination by physicians. This study is carried out to evaluate a new (remote learning) method for better instruction of physical examination skills to the first-year internal medicine residents as a sample of medical population and using available equipment. Methods: This Quasi-Experiment study was carried out on 11 first-year internal medicine residents in 2019. Educational videos from seven major headlines of internal diseases were provided as an online learning resource for a month. A scoring system based on Objective Structured Clinical Examination (OSCE) using a standard checklist was used to evaluate the knowledge of residents prior to introduction to online resources and also after using online materials. The data were analyzed using the SPSS software, version 22. Paired T tests (P≤ 0.05) were used to analyze the relationships between the variab...
This study was conducted to evaluate the effect of a commercial β-glucan based immunostimulant pr... more This study was conducted to evaluate the effect of a commercial β-glucan based immunostimulant preparation, Dinamune®, in the form of a feed supplement, on the growth performance and lysozyme activity of rainbow trout fry ( Oncorhynchus mykiss ) weighing 1 g. Fish were fed diets containing 0 (control), 0.5, 1 and 1.5 g Dinamune® kg -1 of dry diet for a period of 11 weeks. Body weight was generally increased in fish which were fed diets supplemented with concentrations (1.5 Dinamune®/kg feed) (23.75 ± 7.48), compared with the control (21.38 ± 7.04) (p < 0.05). Specific growth rate was significantly (p < 0.05) the highest in 1.5 g Dinamune® kg -1 of dry diet (4.27 ± 0.40) and the lowest in the control group (4.13 ± 0.41). FCR value was found to be the best in the 1g kg -1 group (0.67 ± 0.17), followed by the 1.5g kg -1 group (0.73 ± 0.23), the 0.5 g kg -1 group (0.80 ± 0.36) and the control group (1.00 ± 0.25). Hepatosomatic index (HSI) showed a significant decrease in 1.5 g Din...
The present study examined the effects of Vitacel raw fiber on great sturgeon (Huso huso) immunoc... more The present study examined the effects of Vitacel raw fiber on great sturgeon (Huso huso) immunocompetent cell population size including lymphocyte and neutrophil populations and lysozyme activity after oral administration of the feed and Vitacel at 1.3% of the feed for 90 days. Generally, a significant increase was seen in the neutrophil count of fish receiving Vitacel as compared to the control group 90 days post treatment (P<0.05). Also, lysozyme activity in fish that received Vitacel was significantly higher than the control group 90 days post treatment (P<0.05). The results suggest that Vitacel may effectively promote immune responses in great sturgeon (Huso huso).
A total of 2,400 Persian sturgeon fingerlings weighing 3.50-3.80 g were fed a diet composed of bu... more A total of 2,400 Persian sturgeon fingerlings weighing 3.50-3.80 g were fed a diet composed of but different amounts of D-pro probiotics (Bacillus subtilis, B. licheniformis and yeast Saccharomyces cerevisiae). Fish were distributed in fiberglass tanks (1.80 × 1.80 × 60 cm) each containing 200 fish for 2 months. This research was conducted in Beheshti Sturgeon Hatchery Center, North Iran in the summer of 2016. The mean temperature of the water (21±2℃), pH (7.5±0.5), and aeration and oxygen concentration (6±0.5 mg L-1) were measured for 60 days. The calculated data were analyzed using one-way ANOVA test. Duncan test was used for comparing the means of the treatment. Results suggest that adding probiotics and yeasts to the diet had a significant impact on the percentages of hematocrit (PCV %), neutrophils and lysozyme (p<0.05). In addition, it was shown that the immunoglobulins in the T1 and T3 increased relatively to the control group. The amount of C3 and C4 complements were sign...
Background: The most common complications of multiple sclerosis (MS) are fatigue, muscle cramps, ... more Background: The most common complications of multiple sclerosis (MS) are fatigue, muscle cramps, tremore, squint, imbalance and gait disorders. Doing aquatic aerobics exercises lead to an increase in both physical strength as well as the domain of body movement in M.S patients. In addition, making the balance against the risk factors would decrease the risk of getting cardiovascular disease. Aim: Toinvestigate the effect of aquatic aerobics exercise on some of cardiovascular risk factors in female MS patients. Method: In this quasi-experimental study, 40 female MS patients on the basis of illness degree were allocated randomly into two groups. The experimental group participated in the exercise program for 8 weeks, each week three sessions with intensity of 50-60 percent of the maximum heart rate. The cardiovascular risk factors were measured in experimental and control groups before and after exercise. Blood cholesterol, triglyceride, LDL and HDL were also measured. The collected ...
With considering the abundant sources of Kilka fish in Caspian Sea, the aim of this study was to ... more With considering the abundant sources of Kilka fish in Caspian Sea, the aim of this study was to produce a new product from Kilka in industrial scale with two different types of batters (simple and tempura batter) in order to increase human consumption and evaluation of chemical, microbial and sensory properties.The Kilka fish were battered in the simple and tempura batter, then the fish were breaded with conventional breading crumbs and then pre-fried at 170°C for 30 sec in sunflower oil .The fried samples were immediately frozen through continuous method at -40°C in a spiral freezer and they were packaged. The samples were kept at -18°C. Chemical, microbial and sensory properties were performed on the fried breaded Kilka fish a day after freezing. There were no significant difference found in protein, fat and ash contents of raw breaded Kilka prepared from tempura and simple batters (P≥0.05) But there was significant difference in moisture content of raw breaded Kilka prepare...
This study was conducted to evaluate the antifungal effects of garlic (Allium sativum) and wormwo... more This study was conducted to evaluate the antifungal effects of garlic (Allium sativum) and wormwood (Artemisia sieberi) ethanolic extracts on survival of rainbow trout (Oncorhynchus mykiss) eggs during the incubation and larval stages. The eggs were exposed, in triplicate, to three different doses of garlic (50, 100, 200 ppm) and wormwood (25, 50, 100 ppm) for 30 min under static condition. Malachite green (2 ppm) was also applied as positive control, while none pharmacological intervention was set as negative treatment. The results illustrated no significant difference in eyeing eggs, green eggs and larval stage mortality rate among the treatments (p<0.05). the fish eggs exposed to 2 ppm malachite green, 200 ppm garlic extract, and 100 ppm wormwood had the highest survival rate over the eyeing and hatching stages. Similar results were also obtained in larval stage with the highest survival rate in larvae exposed to 2 ppm malachite green (94.3±0.1 %) followed by those exposed to ...
In this research the effect of alga Spirulina platensis and bacteria Lactococcus lactis on growth... more In this research the effect of alga Spirulina platensis and bacteria Lactococcus lactis on growth indices in juvenile fishes of sturgeon (Acipenser stellatus) was studied and compared together. For this aim 210 juvenile fishes with average weight of 10.75±0.07 gr and density of 10 fishes in each fiber glass tank with the approximate volume of 1000 liter were kept for 60 days. Considering research includes 6 experimental treatments and control treatment done by 3 repeat. Treatments 1, 2 and 3 include 0.2, 0.3, and 0.4 gr of spirulina algae per kilogram diet and treatments 4, 5 and 6 include probiotic at the three levels of 0.2, 0.3 and 0.4 gr of bacteria (10CFU/g) per kilogram diet during experiment. Biometry of fishes was done once every 10 days. The result showed that final average weight, daily growth, body weight increase in first treatment comparing to other treatments and control treatment has devoted the highest amount to itself(P<0.05). Statistical considerations denotes l...
Inclusion of dietary herbal supplements in aquafeed can be considered as a growth promoter and en... more Inclusion of dietary herbal supplements in aquafeed can be considered as a growth promoter and enhancer of immunity system in preventing various fish infections. Therefore, this experiment was conducted to evaluate the effects of different levels of purslane (Portulaca oleracea) extract (POE) on growth performance, hematological indices and immune responses of rainbow trout fry. The fries with initial weight of 3.3±0.1g were divided into four groups with feeding diets supplemented containing 0% (control, T0), 0.5% (T1), 1% (T2), 1.5% (T3) and 2% (T4) of POE for 56 days. After eight weeks of feeding trail, enriched POE diets significantly increased red blood cell count, white blood cell count, hematocrit, hemoglobin concentration, mean corpuscular hemoglobin (MCHC), neutrophil and monocyte percentages, especially in T3 group compared to the control group (p <0.05). Although no significant differences were observed between eosinophil percentage and MCHC, but lymphocyte percentage d...
Heavy metal pollution of aquatic ecosystem and consequently accumulation of these metals in aquat... more Heavy metal pollution of aquatic ecosystem and consequently accumulation of these metals in aquatic organism tissues is one of the recent concerns in seafood industries. Current study has been focused on concentration of some essential and non–essential (toxic) heavy metals in different tissues of the Caspian Kutum, Rutilus kutum and Caspian Roach, Rutilus rutilus as species for human consumption, that are very dangerous for environment. A total of 100 fish samples of two species, Rutilus kutum and Rutilus rutilus were caught from Babolsar and Tonekabon stations (in the southeast of Caspian Sea) during fishing seasons in 2011 and 2012. The muscle, liver and gonad samples from two species were carefully dissected for the determination of heavy metals, Copper, Lead and Cadmium levels. Heavy metals studied at this research are hazardous substances in the environment. Levels of heavy metals were determined using Atomic Absorption Spectrophotometer and statistical analysis was carried ou...
Nonylphenol (NP) is an endocrine disrupting chemical which has been shown to be able to modulate ... more Nonylphenol (NP) is an endocrine disrupting chemical which has been shown to be able to modulate endocrine system of various organisms by different mechanisms. The objective of this study was to investigate the potential effects of 4-NP and 17-β-estradiol (E2) on immune parameters (IgM level and lysozyme activity) of Koi carp (Cyprinus the carpio carpio) in an attempt to expand the knowledge of the immune-reproductive system interactions. Fish were injected with ascending doses (10, 50,100 μg/g-1 body weight) of 4-nonylphenol (4-NP) and (2 μg/g-1 body mass) of 17-β-estradiol (E2) by vehicle during 3a weeks experiment. After 21 days, the fishes (180) were anesthetized, blood samples collected from their caudal vein, and after dissection, theywere grouped into male and female according to their gonadal characters. The results of plasmatic immune parameters measurement showed that 4-NP induced significant increase in IgM levels and lysozyme activity at 50μg/g -1 dosagewhile the ...
BACKGROUNDS: Optimization of microbial compositions and load in live food during the process of b... more BACKGROUNDS: Optimization of microbial compositions and load in live food during the process of bioencapsulation is one of the most important concerns in aquaculture, as it can promote the growth and feeding parameters of fish larvae. OBJECTIVES: The aim of this study was to determine the growth and feeding performance of Persian sturgeon larvae fed with bioencapsulated D. magna with Saccharomyces cerevisiae. METHODS: D. magna were bioencapsulated with S. cerevisiae at three concentrations of 5, 5.30 and 5.48 Log CFU ml-1 for 10 hours. P. sturgeon larvae were fed using enriched D. magna at 30 percent of their body weight, six times a day. Controlled treatment was fed on unbioencapsulated D. magna. RESULTS: The results indicated that the S. cerevisiae promoted the growth and feeding parameters in P. sturgeon larvae. The final body weight and specific growth rate (SGR) in experimental treatments had significant difference in comparison with control treatment (p
This study was conducted to evaluate the effects of alfalfa (Medicago sativa) extract on growth p... more This study was conducted to evaluate the effects of alfalfa (Medicago sativa) extract on growth performance, feed intake, body composition and some of the serum parameters of Rainbow trout (Oncorhynchus mykiss). In this study, a total of 240 rainbow trout were weighted average 8/36±0.2(g) and randomly distributed in four treatments (control), 0.5, 1, 1.5% of extract per (kg) of diet with three replications in plastic tanks. Fish were placed and fed with extract containing food for 60 days. During this time, physicochemical factors of water were measured. At the end of the period, growth index and serum parameters calculated. The results showed that growth performance in treatments that fed with 1% alfalfa extract had a significant difference compared to control treatment (p<0/05). In the indexes of body compositions, an increase in the amount of fat was observed in 1% treatment of the extract, while there was a significant difference with the control group (p<0/05). Serum parameters showed that there were significant differences between fish fed with alcoholic extract of alfalfa and control group (p<0/05). The lowest cholesterol and triglyceride levels and the highest level of protein were observed in the 1.5% extract group. Also, the highest amount of glucose was observed in control treatment. The results of this study showed that the addition of alcoholic extract of alfalfa (1 -1.5 %) to fish diet can improve the growth performance and blood biochemical parameters of rainbow trout.
The Iranian Journal of Veterinary Science and Technology, 2017
The effects of orally administered purple cone flower Echinacea purpura and garlic (Allium satviu... more The effects of orally administered purple cone flower Echinacea purpura and garlic (Allium satvium) on the non-specific immunity and growth condition of beluga weighing 1.15 ± 0.02 kg were evaluated for a period of 60 days. The tree groups of fish were fed two diets containing 0.5% purple coneflower and 1% garlic and a control diet containing no supplemented herb. The growth performance was positively affected by both dietary herbal supplementations (p < 0.05). Among the humoral factors, serum alternative complement, lysozyme and respiratory burst activity increased significantly in all supplemented groups when compared to the control (p < 0.05). These findings demonstrate that dietary Echinacea and garlic can modulate some of the innate defense mechanisms, hematological and growth parameters in Huso huso.
This study aimed to examine the effects of Chlorella vulgaris (CV) on the hematological and immun... more This study aimed to examine the effects of Chlorella vulgaris (CV) on the hematological and immune parameters of Koi carp ( Cyprinus carpio ). An experiment was designed with five treatments, including diets with 0, 2, 5, 7 and 10% of C. vulgaris each with three replicates for 8 weeks. At the end of experiment, the hematological and, immune system parameters were measured and analyzed. The results revealed that supplementation of C. vulgaris in diets resulted in higher percentage of haemoglobuling (Hb), haematocrit (Hct), red blood cells (RBC) and white blood cells (WBC). In addition, a significant increase in the levels of IgM, lysozyme and C4 complement levels were found in fish fed CV at different levels showing a positive effect by this microalgea on the immune status of Koi carp. Based on the results, supplementation of CV particularly at 5% of its dry powder in diet of Koi carp can play an important role in the stimulation of fish immune system.
Journal of Agricultural Science and Technology, 2013
This study pursued the molecular identification of fish species from processed products for human... more This study pursued the molecular identification of fish species from processed products for human consumption which, a priori, belonged to nine species. DNA barcoding using direct sequencing of about 650 bp of the mitochondrial gene cytochrome oxidase subunit I (COI) revealed incorrect labeling in the three Alaska Pollack samples (11% of all samples). Substitution of fish species constitutes serious economic fraud, and our results increase concern regarding the trading of processed fish products in Iran from both health and conservation points of view.
The aim of this study was to investigate the effects of slow and quick freezing on the changes in... more The aim of this study was to investigate the effects of slow and quick freezing on the changes in sensory properties, drip loss, microstructure and proximate compositions of Nile Tilapia (Oreochromis niloticus) fillets. For this reason, skined off and deboned tilapia fillets were frozen by slow and quick freezing methods. The samples were packed and stored at-18 °C for six months. Proximate composition, drip loss, and sensory evaluation of the samples were determined on a montly basis. Microstructure of the samples was studied using Scanning Electron Microscopy (SEM) every second month. Results indicated that fresh tilapia fillets had 1/30, 18/70, 1/85, 79/12 percentage of fat, protein, ash and moisture contents, respectively. The amounts of proximate compositions were changed during the storageperiod. Quick frozen samples had significantly lower changes than slow frozen samples. The percentage of the drip in the slow frozen samples was significantly higher than quick frozen samples. SEM micrographs were also showed that the changes in the microstructure of the samples were different in the slow and frozen samples. Slow freezing method resulted in the higher damage in the microstructure of the samples than quick freezing method. Sensory evaluation of the samples indicated a better acceptability for the quick frozen samples than that for slow frozen sample.
Lysozymes are key molecules of innateimmunity act as non-specific innate-immunity molecules again... more Lysozymes are key molecules of innateimmunity act as non-specific innate-immunity molecules against the invasion of bacteria pathogens in fish.The aim of the present study was to amplify and express a full-length g-type lysozyme cDNA from the skin mucus of common carp (Cyprinus carpio).In this respect, the complete cDNA consisted of an open reading frame of 555 bp which encoded a protein of 185 amino acids. Regardingly, conserved and catalytic residues of this cDNA were identified which showed some similarity with Danio rerio , Salmo salar , Gallus gallus , Xenopus laevisand Tetradon nigroviridis. This Recombinant g-type lysozyme cDNA has been cloned into pMALc2x and expressed in E. colistrain TG1. The resulted recombinant protein showed lytic activity against Micrococcus lysodeikticus.
Background: The recent technological advancement has regrettably resulted in the preference of pa... more Background: The recent technological advancement has regrettably resulted in the preference of para clinical studies over physical examination by physicians. This study is carried out to evaluate a new (remote learning) method for better instruction of physical examination skills to the first-year internal medicine residents as a sample of medical population and using available equipment. Methods: This Quasi-Experiment study was carried out on 11 first-year internal medicine residents in 2019. Educational videos from seven major headlines of internal diseases were provided as an online learning resource for a month. A scoring system based on Objective Structured Clinical Examination (OSCE) using a standard checklist was used to evaluate the knowledge of residents prior to introduction to online resources and also after using online materials. The data were analyzed using the SPSS software, version 22. Paired T tests (P≤ 0.05) were used to analyze the relationships between the variab...
This study was conducted to evaluate the effect of a commercial β-glucan based immunostimulant pr... more This study was conducted to evaluate the effect of a commercial β-glucan based immunostimulant preparation, Dinamune®, in the form of a feed supplement, on the growth performance and lysozyme activity of rainbow trout fry ( Oncorhynchus mykiss ) weighing 1 g. Fish were fed diets containing 0 (control), 0.5, 1 and 1.5 g Dinamune® kg -1 of dry diet for a period of 11 weeks. Body weight was generally increased in fish which were fed diets supplemented with concentrations (1.5 Dinamune®/kg feed) (23.75 ± 7.48), compared with the control (21.38 ± 7.04) (p < 0.05). Specific growth rate was significantly (p < 0.05) the highest in 1.5 g Dinamune® kg -1 of dry diet (4.27 ± 0.40) and the lowest in the control group (4.13 ± 0.41). FCR value was found to be the best in the 1g kg -1 group (0.67 ± 0.17), followed by the 1.5g kg -1 group (0.73 ± 0.23), the 0.5 g kg -1 group (0.80 ± 0.36) and the control group (1.00 ± 0.25). Hepatosomatic index (HSI) showed a significant decrease in 1.5 g Din...
The present study examined the effects of Vitacel raw fiber on great sturgeon (Huso huso) immunoc... more The present study examined the effects of Vitacel raw fiber on great sturgeon (Huso huso) immunocompetent cell population size including lymphocyte and neutrophil populations and lysozyme activity after oral administration of the feed and Vitacel at 1.3% of the feed for 90 days. Generally, a significant increase was seen in the neutrophil count of fish receiving Vitacel as compared to the control group 90 days post treatment (P<0.05). Also, lysozyme activity in fish that received Vitacel was significantly higher than the control group 90 days post treatment (P<0.05). The results suggest that Vitacel may effectively promote immune responses in great sturgeon (Huso huso).
A total of 2,400 Persian sturgeon fingerlings weighing 3.50-3.80 g were fed a diet composed of bu... more A total of 2,400 Persian sturgeon fingerlings weighing 3.50-3.80 g were fed a diet composed of but different amounts of D-pro probiotics (Bacillus subtilis, B. licheniformis and yeast Saccharomyces cerevisiae). Fish were distributed in fiberglass tanks (1.80 × 1.80 × 60 cm) each containing 200 fish for 2 months. This research was conducted in Beheshti Sturgeon Hatchery Center, North Iran in the summer of 2016. The mean temperature of the water (21±2℃), pH (7.5±0.5), and aeration and oxygen concentration (6±0.5 mg L-1) were measured for 60 days. The calculated data were analyzed using one-way ANOVA test. Duncan test was used for comparing the means of the treatment. Results suggest that adding probiotics and yeasts to the diet had a significant impact on the percentages of hematocrit (PCV %), neutrophils and lysozyme (p<0.05). In addition, it was shown that the immunoglobulins in the T1 and T3 increased relatively to the control group. The amount of C3 and C4 complements were sign...
Background: The most common complications of multiple sclerosis (MS) are fatigue, muscle cramps, ... more Background: The most common complications of multiple sclerosis (MS) are fatigue, muscle cramps, tremore, squint, imbalance and gait disorders. Doing aquatic aerobics exercises lead to an increase in both physical strength as well as the domain of body movement in M.S patients. In addition, making the balance against the risk factors would decrease the risk of getting cardiovascular disease. Aim: Toinvestigate the effect of aquatic aerobics exercise on some of cardiovascular risk factors in female MS patients. Method: In this quasi-experimental study, 40 female MS patients on the basis of illness degree were allocated randomly into two groups. The experimental group participated in the exercise program for 8 weeks, each week three sessions with intensity of 50-60 percent of the maximum heart rate. The cardiovascular risk factors were measured in experimental and control groups before and after exercise. Blood cholesterol, triglyceride, LDL and HDL were also measured. The collected ...
With considering the abundant sources of Kilka fish in Caspian Sea, the aim of this study was to ... more With considering the abundant sources of Kilka fish in Caspian Sea, the aim of this study was to produce a new product from Kilka in industrial scale with two different types of batters (simple and tempura batter) in order to increase human consumption and evaluation of chemical, microbial and sensory properties.The Kilka fish were battered in the simple and tempura batter, then the fish were breaded with conventional breading crumbs and then pre-fried at 170°C for 30 sec in sunflower oil .The fried samples were immediately frozen through continuous method at -40°C in a spiral freezer and they were packaged. The samples were kept at -18°C. Chemical, microbial and sensory properties were performed on the fried breaded Kilka fish a day after freezing. There were no significant difference found in protein, fat and ash contents of raw breaded Kilka prepared from tempura and simple batters (P≥0.05) But there was significant difference in moisture content of raw breaded Kilka prepare...
This study was conducted to evaluate the antifungal effects of garlic (Allium sativum) and wormwo... more This study was conducted to evaluate the antifungal effects of garlic (Allium sativum) and wormwood (Artemisia sieberi) ethanolic extracts on survival of rainbow trout (Oncorhynchus mykiss) eggs during the incubation and larval stages. The eggs were exposed, in triplicate, to three different doses of garlic (50, 100, 200 ppm) and wormwood (25, 50, 100 ppm) for 30 min under static condition. Malachite green (2 ppm) was also applied as positive control, while none pharmacological intervention was set as negative treatment. The results illustrated no significant difference in eyeing eggs, green eggs and larval stage mortality rate among the treatments (p<0.05). the fish eggs exposed to 2 ppm malachite green, 200 ppm garlic extract, and 100 ppm wormwood had the highest survival rate over the eyeing and hatching stages. Similar results were also obtained in larval stage with the highest survival rate in larvae exposed to 2 ppm malachite green (94.3±0.1 %) followed by those exposed to ...
In this research the effect of alga Spirulina platensis and bacteria Lactococcus lactis on growth... more In this research the effect of alga Spirulina platensis and bacteria Lactococcus lactis on growth indices in juvenile fishes of sturgeon (Acipenser stellatus) was studied and compared together. For this aim 210 juvenile fishes with average weight of 10.75±0.07 gr and density of 10 fishes in each fiber glass tank with the approximate volume of 1000 liter were kept for 60 days. Considering research includes 6 experimental treatments and control treatment done by 3 repeat. Treatments 1, 2 and 3 include 0.2, 0.3, and 0.4 gr of spirulina algae per kilogram diet and treatments 4, 5 and 6 include probiotic at the three levels of 0.2, 0.3 and 0.4 gr of bacteria (10CFU/g) per kilogram diet during experiment. Biometry of fishes was done once every 10 days. The result showed that final average weight, daily growth, body weight increase in first treatment comparing to other treatments and control treatment has devoted the highest amount to itself(P<0.05). Statistical considerations denotes l...
Inclusion of dietary herbal supplements in aquafeed can be considered as a growth promoter and en... more Inclusion of dietary herbal supplements in aquafeed can be considered as a growth promoter and enhancer of immunity system in preventing various fish infections. Therefore, this experiment was conducted to evaluate the effects of different levels of purslane (Portulaca oleracea) extract (POE) on growth performance, hematological indices and immune responses of rainbow trout fry. The fries with initial weight of 3.3±0.1g were divided into four groups with feeding diets supplemented containing 0% (control, T0), 0.5% (T1), 1% (T2), 1.5% (T3) and 2% (T4) of POE for 56 days. After eight weeks of feeding trail, enriched POE diets significantly increased red blood cell count, white blood cell count, hematocrit, hemoglobin concentration, mean corpuscular hemoglobin (MCHC), neutrophil and monocyte percentages, especially in T3 group compared to the control group (p <0.05). Although no significant differences were observed between eosinophil percentage and MCHC, but lymphocyte percentage d...
Heavy metal pollution of aquatic ecosystem and consequently accumulation of these metals in aquat... more Heavy metal pollution of aquatic ecosystem and consequently accumulation of these metals in aquatic organism tissues is one of the recent concerns in seafood industries. Current study has been focused on concentration of some essential and non–essential (toxic) heavy metals in different tissues of the Caspian Kutum, Rutilus kutum and Caspian Roach, Rutilus rutilus as species for human consumption, that are very dangerous for environment. A total of 100 fish samples of two species, Rutilus kutum and Rutilus rutilus were caught from Babolsar and Tonekabon stations (in the southeast of Caspian Sea) during fishing seasons in 2011 and 2012. The muscle, liver and gonad samples from two species were carefully dissected for the determination of heavy metals, Copper, Lead and Cadmium levels. Heavy metals studied at this research are hazardous substances in the environment. Levels of heavy metals were determined using Atomic Absorption Spectrophotometer and statistical analysis was carried ou...
Nonylphenol (NP) is an endocrine disrupting chemical which has been shown to be able to modulate ... more Nonylphenol (NP) is an endocrine disrupting chemical which has been shown to be able to modulate endocrine system of various organisms by different mechanisms. The objective of this study was to investigate the potential effects of 4-NP and 17-β-estradiol (E2) on immune parameters (IgM level and lysozyme activity) of Koi carp (Cyprinus the carpio carpio) in an attempt to expand the knowledge of the immune-reproductive system interactions. Fish were injected with ascending doses (10, 50,100 μg/g-1 body weight) of 4-nonylphenol (4-NP) and (2 μg/g-1 body mass) of 17-β-estradiol (E2) by vehicle during 3a weeks experiment. After 21 days, the fishes (180) were anesthetized, blood samples collected from their caudal vein, and after dissection, theywere grouped into male and female according to their gonadal characters. The results of plasmatic immune parameters measurement showed that 4-NP induced significant increase in IgM levels and lysozyme activity at 50μg/g -1 dosagewhile the ...
BACKGROUNDS: Optimization of microbial compositions and load in live food during the process of b... more BACKGROUNDS: Optimization of microbial compositions and load in live food during the process of bioencapsulation is one of the most important concerns in aquaculture, as it can promote the growth and feeding parameters of fish larvae. OBJECTIVES: The aim of this study was to determine the growth and feeding performance of Persian sturgeon larvae fed with bioencapsulated D. magna with Saccharomyces cerevisiae. METHODS: D. magna were bioencapsulated with S. cerevisiae at three concentrations of 5, 5.30 and 5.48 Log CFU ml-1 for 10 hours. P. sturgeon larvae were fed using enriched D. magna at 30 percent of their body weight, six times a day. Controlled treatment was fed on unbioencapsulated D. magna. RESULTS: The results indicated that the S. cerevisiae promoted the growth and feeding parameters in P. sturgeon larvae. The final body weight and specific growth rate (SGR) in experimental treatments had significant difference in comparison with control treatment (p
This study was conducted to evaluate the effects of alfalfa (Medicago sativa) extract on growth p... more This study was conducted to evaluate the effects of alfalfa (Medicago sativa) extract on growth performance, feed intake, body composition and some of the serum parameters of Rainbow trout (Oncorhynchus mykiss). In this study, a total of 240 rainbow trout were weighted average 8/36±0.2(g) and randomly distributed in four treatments (control), 0.5, 1, 1.5% of extract per (kg) of diet with three replications in plastic tanks. Fish were placed and fed with extract containing food for 60 days. During this time, physicochemical factors of water were measured. At the end of the period, growth index and serum parameters calculated. The results showed that growth performance in treatments that fed with 1% alfalfa extract had a significant difference compared to control treatment (p<0/05). In the indexes of body compositions, an increase in the amount of fat was observed in 1% treatment of the extract, while there was a significant difference with the control group (p<0/05). Serum parameters showed that there were significant differences between fish fed with alcoholic extract of alfalfa and control group (p<0/05). The lowest cholesterol and triglyceride levels and the highest level of protein were observed in the 1.5% extract group. Also, the highest amount of glucose was observed in control treatment. The results of this study showed that the addition of alcoholic extract of alfalfa (1 -1.5 %) to fish diet can improve the growth performance and blood biochemical parameters of rainbow trout.
The Iranian Journal of Veterinary Science and Technology, 2017
The effects of orally administered purple cone flower Echinacea purpura and garlic (Allium satviu... more The effects of orally administered purple cone flower Echinacea purpura and garlic (Allium satvium) on the non-specific immunity and growth condition of beluga weighing 1.15 ± 0.02 kg were evaluated for a period of 60 days. The tree groups of fish were fed two diets containing 0.5% purple coneflower and 1% garlic and a control diet containing no supplemented herb. The growth performance was positively affected by both dietary herbal supplementations (p < 0.05). Among the humoral factors, serum alternative complement, lysozyme and respiratory burst activity increased significantly in all supplemented groups when compared to the control (p < 0.05). These findings demonstrate that dietary Echinacea and garlic can modulate some of the innate defense mechanisms, hematological and growth parameters in Huso huso.
This study aimed to examine the effects of Chlorella vulgaris (CV) on the hematological and immun... more This study aimed to examine the effects of Chlorella vulgaris (CV) on the hematological and immune parameters of Koi carp ( Cyprinus carpio ). An experiment was designed with five treatments, including diets with 0, 2, 5, 7 and 10% of C. vulgaris each with three replicates for 8 weeks. At the end of experiment, the hematological and, immune system parameters were measured and analyzed. The results revealed that supplementation of C. vulgaris in diets resulted in higher percentage of haemoglobuling (Hb), haematocrit (Hct), red blood cells (RBC) and white blood cells (WBC). In addition, a significant increase in the levels of IgM, lysozyme and C4 complement levels were found in fish fed CV at different levels showing a positive effect by this microalgea on the immune status of Koi carp. Based on the results, supplementation of CV particularly at 5% of its dry powder in diet of Koi carp can play an important role in the stimulation of fish immune system.
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Papers by Mehdi Soltani