Papers by Md. Mostavi Enan Eshik
PeerJ, 2022
Shrimp aquaculture has been accomplished with breeding and nursing of shrimp in an artificial env... more Shrimp aquaculture has been accomplished with breeding and nursing of shrimp in an artificial environment to fulfill the increasing demand of shrimp consumption worldwide. However, the microbial diseases appear as a serious problem in this industry. The study was designed to identify the diverse bacteria from shrimp PL (post-larvae) nurseries and to profile antibiotic resistance patterns. The rearing water (raw seawater, treated and outlet water) and shrimp PL were collected from eight nurseries of south-west Bangladesh. Using selective agar plates, thirty representative isolates were selected for 16S rRNA gene sequencing, antibiotic susceptibility test and MAR index calculation. Representative isolates were identified as Aeromonas caviae, Pseudomonas monteilii, Shewanella algae, Vibrio alginolyticus, V. brasiliensis, V. natriegens, V. parahaemolyticus, V. shilonii, V. xuii, Zobellella denitrificans which are Gram-negative, and Bacillus licheniformis and B. pumilus which are Gram-po...
Dhaka University Journal of Biological Sciences, 2020
To identify the gut bacteria of Pangasianodon hypophthalmus (striped catfish; Pangas) using class... more To identify the gut bacteria of Pangasianodon hypophthalmus (striped catfish; Pangas) using classical and molecular approach was carried out. Total bacterial count (TBC) in the gut of pangas from farm and market samples were found 5.07 ± 1.70 × 106 and 1.40 ± 0.47 × 106 cfu/g, respectively. The gut microbiota of pangas was dominated by members of the Gram-negative genera. Only three isolates (MyF1/1, MyF1/4 and GaW1/2) were found to be Gram-positive among the 16 representative isolates. Using 16S rRNA gene sequencing; Bacillus, Macrococcus, Citrobacter, Aeromonas, Proteus, Klebsiella, Enterobacter, Escherichia and Edwardsiella were found to be associated with the gut of this fish. Among them, Aeromonas was the most dominant genus (5 out of 16). Antibiotic sensitivity pattern reflected that all the isolates were sensitive to gentamycin. Multiple antibiotics resistant isolates were also identified of which MyF3/13 (identified as Citrobacter amalonaticus) was found resistant against se...
Journal of Invertebrate Pathology, 2021
White spot disease has caused significant economic losses in the shrimp farming industry of Bangl... more White spot disease has caused significant economic losses in the shrimp farming industry of Bangladesh over the last two decades. The responsible virus, WSSV, may show severe disease with significant mortality depending on farm management and environmental and seasonal changes. Data on farm management and environmental parameters were collected from the southwest region of Bangladesh in 2018, and WSSV infection was confirmed by the species-specific gene VP28 using conventional real-time PCR and sequencing. Through bivariate analysis, nine significant risk factors for WSD were identified, viz. farm age, presence of nursery pond, reservoir of PL, weed in farm area, control of weed, stocking density, stocking frequency, ammonia and oxygen concentration. This study detected 46 WSSV-infected shrimp farms by conventional PCR, whereas real-time PCR identified 47 WSSV-positive out of 49 farms. WSSV prevalence was highest in the Khulna region, with 100% positivity in all seasons. WSSV loads ranged from 5.62×109 to 2.01×1015 copies/g of shrimp tissue. The VP28 gene sequence confirmed that 15 representative samples were 100% identical to the 2018 WSSV strain of India. The relationships among risk factors, prevalence and severity of disease, and origin of WSSV strains could be impactful for WSD management.
Seasonal variations of proximate compositions, amino acids, and fatty acids contents of Monopteru... more Seasonal variations of proximate compositions, amino acids, and fatty acids contents of Monopterus cuchia were studied for the first time in Bangladesh. The fat and fatty acid, protein and amino acid contents showed a significant seasonal dependency. Lipid contents showed the wider variation than the protein contents. Among the 14 recorded amino acids, the primary amino acids were lysine, glutamic acid, arginine, glycine and aspartic acid. The ratio of essential and non-essential amino acids was higher in the post-monsoon season (0.69) than pre-monsoon (0.68) and monsoon season (0.68) and did not show seasonal discrepancies. The amount of fatty acids were found in order of saturated fatty acids (SFA) > mono unsaturated fatty acids (MUFA) > polyunsaturated fatty acids (PUFA). The predominant fatty acids were palmitic and stearic acids of SFAs, oleic and palmitoleic acid of MUFAs and linoleic and α linolenic acids of PUFAs group. The combined value of EPA+DHA varied from 1.44 – ...
Water pollution poses a threat of heavy metal accumulation in aquatic animals. This study was des... more Water pollution poses a threat of heavy metal accumulation in aquatic animals. This study was designed to determine the heavy metals contamination in shrimp PL (post larvae) nurseries of Khulna region and associated human health risks. Water (raw sea water, treated and outlet water) and PL samples of five shrimp nurseries of Khulna districts were analyzed to measure the concentrations of six heavy metals (Zn, Cr, Mn, Cu, Pb and Ni) by using Atomic Absorption Spectrometer (Model No: AA-7000, Shimadzu). The metal concentrations were higher in almost all PL samples of five nurseries except Mn, which value was below detection level (BDL). Concentrations of Zn and Cu were significantly higher (P<0.05) in PL samples than inlet (raw sea water, treated water) and outlet water samples among five nurseries. The highest value for Cr was found in PL sample (2.95±3.64) ppm but the lowest was in outlet water (0.004±0.004) ppm. The highest value of Pb was found in PL samples (3.48±7.78) ppm and...
Exposure and Health, 2021
In this study, pangas and feed samples were analyzed to estimate the levels of metallic elements ... more In this study, pangas and feed samples were analyzed to estimate the levels of metallic elements and to profile the human health risks due to consumption of contaminated fish. This investigation confirmed significant variations in heavy metal concentrations among different tissues of pangas in the order of Ni> Cu> Pb> Cd> Cr in pre-monsoon; and Ni> Cd= Cu> Pb= Cr in post-monsoon. Considerably higher concentrations of Pb, Cu and Cr were estimated in liver; and Cd and Ni were detected in muscle than other organs (p>0.05). Statistically significant higher amount of Cd, Ni, and Cu were observed in pre-monsoon than post-monsoon. Furthermore, three metal pairs showed significant association (Pb-Ni and Pb-Cu involved positively; Cd-Ni acted negatively). In pre-monsoon, Cd, Pb, Ni, Cu concentrations of feed significantly differed than pangas contents; whereas only Cu varied during the post-monsoon. Regression analysis revealed the significant effect of Ni content in feed on the Cu deposition of pangas (p-value 0.027, that was <0.05). For the assessment of potential human health risk of the studied metals, estimated daily intake (EDI), target hazard quotient (THQ), hazard index (HI) and carcinogenic risk (CR) indices were calculated. Studied EDI indicated that an average adult ingested a higher amount of Ni and Cu than the recommended intake limit. Nevertheless, only the higher EDI of Ni increases the value of THQ and HI than standard limit indicates adverse non-carcinogenic risk. However, lower CR of Pb confirmed no serious health hazard due to the ingestion of pangas. Factor analysis through principal component and cluster analysis suggested that higher concentrations of Pb and Ni may regulate by the feed used, geochemical properties or rapid industrialization in the study area. A proper monitoring for controlling the quality of fish feed with sustainable planning for industrialization could secure the booming of pangasius aquaculture in Bangladesh.
Dhaka University Journal of Biological Sciences, 2018
Acute hepatopancreatic necrosis disease (AHPND) is an emerging shrimp disease caused by strains o... more Acute hepatopancreatic necrosis disease (AHPND) is an emerging shrimp disease caused by strains of Vibrio parahaemolyticus containing a unique virulent plasmid, responsible for substantial economic losses since 2009; caused up to 100% mortality in farmed shrimp Penaeus monodon. The purpose of this study was to isolate and identify the pathogenic strain of V. parahaemolyticus causing AHPND in cultured shrimp (Penaeus monodon) using classical and molecular techniques. Samples were collected from three different locations of south-west shrimp farming regions of Bangladesh viz. Sadar Upazilla of Satkhira; Mongla and Morrelganj under Bagerhat district. In this study, three selective media were used for primary isolation of V. parahaemolyticus. Among 46 primary isolates, 18 representative isolates were checked for the species-specific detection of V. parahaemolyticus using ldh primers and all of them were found to be positive. 16S rRNA gene sequencing were used to further confirm the isol...
Journal of the Asiatic Society of Bangladesh, Science, 2019
Black tiger shrimp (Penaeus monodon Fabricius 1798) cultured in Bangladesh was investigated for t... more Black tiger shrimp (Penaeus monodon Fabricius 1798) cultured in Bangladesh was investigated for the presence of microsporidian parasite. Histological section of hepatopancreas showed a large number of microsporidian spores under light microscopy. Spores under scanning electron microscope appeared oval shapes. Histology of infected shrimps showed severe degeneration of hepatopancreatic tubules. Early and late stage of microsporidian parasites in hepatopancreatic tubules were also observed. DNA extracted from the hepatopancreas of shrimps were subjected to PCR amplification using primers targeting microsporidian SSU rRNA gene. The PCR amplified an expected product of ~328 bp and the sequences showed 81 - 82% identity with the Paranucleospora theridion reported from western Norway in 2008. Further screening of field samples was carried out using EHP-specific primers. DNA extracted from ten hepatopancreas samples of P. monodon were tested and none found to be positive for EHP (Enterocyt...
Background Due to its rapid lethal effect in the early stage of shrimp, acute hepatopancreatic ne... more Background Due to its rapid lethal effect in the early stage of shrimp, acute hepatopancreatic necrosis disease (AHPND) causing great economic losses, since it first outbreak in southeast China in 2009. Vibrio parahaemolyticus , carrying the pir A and pir B toxin genes is known to cause AHPND in shrimp. The overall objective of this study was to sequence whole genome of AHPND positive V. parahaemolyticus strains isolated from shrimp ( Peneaus monodon ) of south-west region of Bangladesh in 2016 and 2017 and characterize the genomic features and emergence pattern of this marine pathogen. Results Two targeted AHPND positive V. parahaemolyticus strains were confirmed using PCR with 16S rRNA, ldh , AP3 and AP4 primers. The assembled genomes of strain MSR16 and MSR17 were comprised of total 5,393,740 bp and 5,241,592 bp, respectively. From annotation, several virulence genes involved in chemotaxis and motility, EPS type II secretion system, Type three secretion system-1 (TTSS-1) and its ...
Journal of Bangladesh Academy of Sciences, 2018
Acute hepatopancreatic necrosis disease (AHPND) of shrimps is a bacterial disease, first appeared... more Acute hepatopancreatic necrosis disease (AHPND) of shrimps is a bacterial disease, first appeared in China in 2009 and causes mortality up to 100 % which usually occurs early (within approximately first 35 days) after stocking shrimp fry of black tiger shrimp, Penaeus monodon (Fabricius) and white leg shrimp, Litopenaeus vannamei (Boone). The purpose of this study was to isolate and identify the pathogenic strain of V. parahaemolyticus causing AHPND in cultured shrimps (P. monodon) using molecular techniques. After a disease outbreak in April 2017, shrimp samples were collected from three different locations of south-west shrimp farming region of Bangladesh viz. Satkhira, Khulna and Bagerhat districts. In this study, three selective media were used for primary isolation of V. parahaemolyticus. Among 28 primary isolates, representative14 isolates were checked for the species-specific detection of V. parahaemolyticus using ldh primers and all of them were found to be positive. The iso...
Bangladesh Journal of Zoology, 2018
Cinnamon has different biological properties such as antioxidant, antimicrobial, antidiabetic and... more Cinnamon has different biological properties such as antioxidant, antimicrobial, antidiabetic and antiallergic. This study was carried out to investigate the effect of cinnamon as feed supplement on growth performances and bacterial load of Heteropneustes fossilis. Twenty fries were stocked in each 60 litre aquarium for a rearing period of 90 days. Commercial diet was used that contains 0.0% (control), 0.5%, and 1.0% cinnamon bark powder. The diet containing 1% cinnamon resulted in greater ADG, SGR, FCR and survival rate. Whereas, the diet containing 0.5% cinnamon resulted in greater condition factor and lower FCR. In most of the cases the diet containing 1% cinnamon showed lower amount of bacterial count than the controlled condition. Cinnamon could have an antibacterial activity antagonistic to Vibrio and Aeromonas as there was no count found in fish flesh samples after 90 days of culture. The findings of this study suggest that growth performances and bacterial load of H. fossili...
Journal of the Asiatic Society of Bangladesh, Science, 2021
The freshwater air-breathing swamp eel Monopterus spp. are native to the freshwater of Bangladesh... more The freshwater air-breathing swamp eel Monopterus spp. are native to the freshwater of Bangladesh and throughout the Indian subcontinent. To identify the different swamp eel species and to check the genetic diversity among them, a total of twelve swamp eel specimens were collected from four districts (Tangail, Bogura, Bagerhat and Sylhet) representing the four division of Bangladesh. The extracted DNA from twelve fish samples was amplified by the PCR technique for DNA barcoding and RAPD analysis. Among 12 specimens, 8 specimens showed a 95-100% similarity with M. cuchia species published in the NCBI GenBank database and BOLD system. The studied mct3 (collected from Tangail region), mcs1, mcs2 and mcs3 (collected from Sylhet region) specimens showed about 83% homology with Ophisternon sp. MFIV306-10 as per BLAST search; whereas BOLD private database showed 99% similarity with Ophisternon bengalense (Bengal eel). From the phylogenetic tree analysis, 8 samples were clustered with M. cu...
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Papers by Md. Mostavi Enan Eshik