одређивање специфичних приоритета: идентификација популација које ће обухватити процес конзерваци... more одређивање специфичних приоритета: идентификација популација које ће обухватити процес конзервације 6.6. In situ стратегије конзервације 6.7. организација и планирање специфичне активности у процесу конзервације 6.8. Припрема упустава за управљање објектима конзервације 6.9. избор и управљање областима за in situ конзервацију 6.9.1. број области за конзервацију 6.9.2. Величина сваке површине предвиђене за конзервацију 6.9.3. избор појединачних популација у оквиру сваке генеколошке зоне 6.9.4. израда плана управљања за сваки конзервацијску област 6.10. очувањe шумских генетичких ресурса у заштићеним подручима 6.10.1. јачање улоге заштићених подручја у процесу конзервације шумских генетичких ресурса 7. Ex SItU коНЗеРВацијa и коРиШћење ШУМСког геНеТичког ДиВеРЗиТеТа 7.1. Питања која треба узети у обзир приликом ex situ конзервације 7.2. Стратегија узорковања у процесу ex situ конзервације 7.3. оснивање и управљање ex situ конзервацијским засадима 7.3.1. циљ ex situ конзервације у засадима 7.3.2. Материјал и методе за успостављање ex situ конзервацијских засада 7.3.3. Планирање ex situ конзервационих засада 7.3.4. Успостављање ex situ засада 7.4. Ex situ конзервација путем складиштења и коришћења 7.4.1. еx situ конзервација путем складиштења сјемена 7.4.2. криопрезервација сјемена и клијанаца 7.4.3. In vitro конзервација 7.4.4. Складиштење полена 7.4.5. ДНк складиштење 7.5. опште препоруке за in situ и ex situ очување шумских генетичких ресурса 66 8. оДРжиВо гаЗДоВање ШУМаМа У ВеЗи ШУМСких геНеТичких РеСУРСа 8.1. Системи газдовања и очување шумских генетичких ресурса у различитим типовима шума 8.1.1. Усаглашавање сјеча са очувањем шумских генетичких ресурса 8.2. Праћење и оцјена утицај управљања на шумске генетичке ресурсе 8.3. Управљање осталим (недрвним) шумским производима 8.4. оплемењивање и очување шумских генетичких ресурса 8.4.1. обезбеђивање потенцијала за коришћење у будућности 9. иСТРажиВање и РаЗВој 9.1. Значај специфичних студија за програме практичне конзервације 9.2. гдје се налазе преостале популације, и који је статус конзервације одабраних врста? 9.3. истраживачки рад који треба да помогне у доношењу одлука о конзервацији популација 9.4. колико велика јединица конзервације треба да буде? 9.5. информације и истраживачки рад који потпомажу развој смјерница за управљање и коришћење конзервираних популација 9.6. Примјена молекуларних истраживања у процесу очувања генетичких ресурса 9.7. гдје ставити акценат у истраживачком раду?
Metadata Excel sheets of the data published under the title of "A genomic data set of single... more Metadata Excel sheets of the data published under the title of "A genomic data set of single‐nucleotide polymorphisms (SNPs) generated by RAD tag sequencing in <em>Q. petraea</em> (Matt.) Liebl. populations from Central-Eastern Europe and Balkan Peninsula"
Ex situ conservation is the preservation of components of biological diversity outside their natu... more Ex situ conservation is the preservation of components of biological diversity outside their natural habitats. Such ex situ work is an increasingly important component of conservation and restoration efforts. Its primary objective is to support conservation by ensuring the survival of threatened species and the maintenance of associated genetic diversity. To do so, ex situ institutions preserve the genetic or reproductive material of target species, or take care of the living target species for the purpose of reintroduction. Ex situ conservation measures should support in situ conservation measures. Organized ex situ conservation activities in Bosnia and Herzegovina (BiH) started in the middle of the last century. Activities were stopped during the 1990s because of the civil war, and then after 2000, the ex situ conservation of species continued. Ex situ cultures in BiH are established within arboreta, live archives, provenance tests, progeny tests, seed orchards and clone tests.
The total area under protection on the territory of Bosnia and Herzegovina is around 2% with tend... more The total area under protection on the territory of Bosnia and Herzegovina is around 2% with tendency to increase. The conservation of forest ecosystems “in situ” in Bosnia and Herzegovina is comprises: natural reserves, national parks, groups of trees or individual trees and seed stands. There are 2 Strict Nature Reserves, 4 National Parks, 16 Natural Monuments, 5 Protected Landscape and 2 protected areas with sustainable use of natural resources. The total area of delineated forest seed sources is 2154.93 ha 212 which consist of 121 coniferous and 88 deciduous species. The management in all in-situ areas is prescribed by law and rulebooks.
The paper presents the results of investigation of variability of net photosynthesis (A), transpi... more The paper presents the results of investigation of variability of net photosynthesis (A), transpiration (E), stomatal conductance (gs) and water use efficiency (WUE) in one year old seedlings of wild cherry (Prunus avium L.), assessed in a nursery trial. Study involved 5 families of half-sib progenies which originate from the Northern part of Serbia (Vojvodina Province). The results showed significant differences among families in regards of stomatal con-ductance (0.163-0.256 mol m-2 s-1), transpiration (3.27-5.28 mmol m-2 s-1) and water use efficiency (1.98-3.80 μmol mmol −1) (p≤0.001), while differences regarding net photosynthesis (10.49-12.44 μmol m-2 s-1) were not statistically significant (p≤0.124). Canonical discriminant analysis (CDA) was performed in order to estimate mul-tivariate relations among analyzed leaf photosyntetical traits. Families were separated by the first canonical axis (CD1), which described 82% of variability. Presence of differences in regards of E, gs an...
This paper presents the results of studies on protein polymorphism in seeds of Scots pine (Pinus ... more This paper presents the results of studies on protein polymorphism in seeds of Scots pine (Pinus sylvestris L.) and Austrian pine (Pinus nigra Arn.) as the most important economic species of the genus Pinus in Serbia. Polymorphism of protein markers was determined in selected genotypes originating from seven populations (Scots pine) and six populations (Austrian pine). Analysis of protein markers was performed using two statistical methods, NTSYS and correspondence analysis. Both methods give the same arrangement of the analyzed populations, whereby, because of a different view of genetic distances, they can and should be combined, 478
dynamics of germination and morphometrics properties of Austrian pine (Pinus nigra Arnold) saplin... more dynamics of germination and morphometrics properties of Austrian pine (Pinus nigra Arnold) saplings in terms of early indicators of tolerance toward the drought- Genetika, Vol 43, No. 1,75-90. Genetic markers, from morphological to molecular, in function with early indicators of tolerance toward drought, have been an object of research and scientific papers for many years. It starts with the hypothesis that seedlings produced from seeds that were collected from population of extremely different site conditions, on the level of open pollinated families, will have different results concerning drought tolerance. By tracking the 76 GENETIKA, Vol. 43, No. 1, 75-90, 2011 dynamics of germination and morphological parameters of saplings, we are researching the interdependence of these parameters with the survival and growth of two-year-old seedlings in conditions where there is a lack of water. Austrian Pine seeds were collected from forty trees, distributed over five provenances on the Bal...
According to the data obtained after the Second National Forest Inventory in Bosnia and Herzegovi... more According to the data obtained after the Second National Forest Inventory in Bosnia and Herzegovina (2006–2009) was carried out, the total area of forests and forest land in Bosnia and Herzegovina (BiH) is 3,231,500 ha or 63.08%, whilst the area covered by forest takes up 2,904,600 ha, or 56.7% of the total Bosnia and Herzegovina land area The land area of BiH is divided into four ecological vegetation areas (Pripanon, Inner Dinarides, Transitional Illyrian-Moesiac area and Mediterranean area). The spatial distribution of the main vegetation types is influenced by horizontal zonation and altitudinal belts. These areas are then followed by the mountainous belt with beech forests, beech and fir, beech and fir with spruce and the subalpine beech forest belt. There is a great number of endemic species and habitats and relic locations – a terrestrial flora and fauna shelter, which are now preserved under specific paleo-climate conditions. BiH is one of the countries in Europe with the highest diversity of both, plants and animals species, which are endangered by climate change. The abundance of diversity is reflected in a large number of plant and animal species within the region (about 4500 higher plants, 600 taxa mosses and about 80 ferns, around 250 species of forest trees and shrubs). This is why the position of BiH is high in the list of European countries of a high biodiversity level. The variability has been investigated by molecular markers for the most important species. The variability is high and it is important for meeting climate changes. Afforestation trends in BiH indicate that the afforestation volume decreased. Establishihng of new forests are not at the satisfactory level due to the lack of financial resources and insufficient attention to the aforested areas.
The total area under protection on the territory of Bosnia and Herzegovina is around 2% with tend... more The total area under protection on the territory of Bosnia and Herzegovina is around 2% with tendency to increase. The conservation of forest ecosystems “in situ” in Bosnia and Herzegovina is comprises: natural reserves, national parks, groups of trees or individual trees and seed stands. There are 2 Strict Nature Reserves, 4 National Parks, 16 Natural Monuments, 5 Protected Landscape and 2 protected areas with sustainable use of natural resources. The total area of delineated forest seed sources is 2154.93 ha 212 which consist of 121 coniferous and 88 deciduous species. The management in all in-situ areas is prescribed by law and rulebooks.
Developing “climate smart forestry” (CSF) indicators in mountain forest regions requires collecti... more Developing “climate smart forestry” (CSF) indicators in mountain forest regions requires collection and evaluation of local data and their attributes. Genetic resources are listed among the core indicators for forest biological diversity. This study is a report on the evaluation of the standing genetic diversity within and across 12 pure beech stands (Fagus sylvatica L.) established within the CLIMO (CLImate Smart Forestry in MOuntain Regions) project, using nuclear microsatellite markers. The sampling sites were set along the species’ distribution range, including the Balkan region and extending towards the Iberian Peninsula. Cores or leaves from 20 to 23 old, mature trees per plot were sampled for DNA analysis. Genetic diversity indices were high across the range (HE = 0.74−0.81) with the highest in the Bosnian Mountains. Genetic divergence increased significantly with the geographical distance (Mantel test: r = 0.81, p < 0.001). Most of the stands exhibited an excess of hetero...
ГЛАСНИК ШУМАРСКОГ ФАКУЛТЕТА УНИВЕРЗИТЕТА У БАЊОЈ ЛУЦИ, 2017
Cilj rada je analiza perioda cvjetanja vrsta roda lipa (Tilia sp.)u toku jedne vegetacione sezone... more Cilj rada je analiza perioda cvjetanja vrsta roda lipa (Tilia sp.)u toku jedne vegetacione sezone, na području grada Banja Luke. Vrsteobuhvaćene ovim istraživanjem su: krupnolisna, sitnolisna i srebrnolisnalipa. Terensko istraživanje je sprovedeno na tri lokaliteta: u parku MladenStojanović, Univerzitetskom gradu i na Starčevici. Utvrđene su klimatske,edafske i orografske karakteristike objekta istraživanja. Navedenilokaliteti, iako se nalaze relativno blizu u istom klimatskom području,pružaju različite ekološke uslove za razvoj i fenologiju istraživanih vrsta.Praćeno je pet faza cvjetanja, s ciljem da se utvrdi uticaj stanišnih uslovana fenofazu cvjetanja, te utvrde genetičke različitosti analiziranih stabala.Rezultati istraživanja pokazuju da početak i trajanje pojedinih fazacvjetanja istraživanih vrsta, u najvećoj mjeri zavise od vrste i stanišnihuslova. Na osnovu izvršenih fenoloških osmatranja konstatovane surazlike u trajanju perioda cvjetanja kod analiziranih stabala unutar i...
ГЛАСНИК ШУМАРСКОГ ФАКУЛТЕТА УНИВЕРЗИТЕТА У БАЊОЈ ЛУЦИ, 2020
Analiziranje kvaliteta sjemena i njegovog klijanja ima veliku važnost u proučavanju varijabilitet... more Analiziranje kvaliteta sjemena i njegovog klijanja ima veliku važnost u proučavanju varijabiliteta pitomog kestena. Ovim analizama se u najranijem uzrastu biljaka može utvrditi obim potencijalne genetičke varijabilnosti vrste. U radu su prikazani rezultati ispitivanja kvaliteta sjemena, direktnim (tehnička klijavost) i indirektnim metodom (ispitivanje vitaliteta embriona pomoću Tetrazolijum testa – TZ-test), za uzorke pitomog kestena iz šest prirodnih populacija sa područja Bosne i Hercegovine (BiH). Ispitivanja su obavljena prema uputstvima „The International Seed Testing Association”. Analizom su obuhvaćene uzastopno dvije kalendarske godine. Utvrđena je niska prosječna vrijednost klijavosti sjemena kao i vitaliteta embriona za sve istraživane populacije u obje godine istraživanja. Poznavanje kvaliteta sjemena važan je faktor za sve koji su uključeni u proces proizvodnje sjemena, a informacija o njegovom kvalitetu omogućava smjernice tokom proizvodnje, trgovine i skladištenja.
The article was published without proper open access tagging on SpringerLink. The original articl... more The article was published without proper open access tagging on SpringerLink. The original article has been corrected. Acknowledgements This designation is without prejudice to positions on status, and is in line with UNSCR 1244 (1999) and the ICJ Opinion's on the Kosovo* Declaration of Independence.
& Key message High levels of genetic diversity, pronounced genetic structure and limitations to g... more & Key message High levels of genetic diversity, pronounced genetic structure and limitations to gene flow in Serbian spruce, a rare and endangered tree species from the refugial Balkan region, point towards a "one population-one unit" strategy for assembling a network of Genetic Conservation Units (GCUs) for its dynamic conservation. On the other hand, genetic information also permits to prioritize populations for conservation based on their contribution to genetic diversity and differentiation. & Context Serbian spruce, Picea omorika (Panč.) Purk., is a rare, IUCN red-listed European conifer endemic to the Balkan region. Its current rigid conservation (without any intervention allowed in~30 remnant populations) and the extant network of Genetic Conservation Units (four natural populations and three planted stands from the western part of the species range, in the Republic of Srpska, Bosnia and Herzegovina, RS-BH) might be ineffective in preserving the species' genetic diversity. & Aims To facilitate implementation of dynamic conservation of Serbian spruce by reassessing the number and size of remnant populations in RS-BH and updating genetic knowledge on these understudied western Serbian spruce populations. & Methods Comprehensive field survey in RS-BH, genotyping 689 individuals from 14 western populations with ten highly informative nuclear EST-SSRs and analytical methods for prioritizing populations for conservation based on their contribution to the geographical structuring of genetic diversity. & Results The genetic diversity of western Serbian spruce populations (Ae = 2.524, H E = 0.451) is comparable with what was found for eastern ones; they are highly genetically differentiated (Hedrick's G' ST = 0.186; Jost's D = 0.097) and comprise ten distinct gene pools. Effective population size is often ≥ 15. As much as 14% of alleles is not preserved in the extant GCUs established in natural populations. Eight populations positively contribute to within-population genetic diversity, four to genetic differentiation, and two are globally important in terms of diversity and differentiation. Although wildfires may contribute to admixture of different gene pools, re-establishment from seeds from extirpated populations has likely prevailed in studied populations. & Conclusions A larger network of GCUs is required for the dynamic conservation of western Serbian spruce populations. A "one population-one unit" strategy, with 14 GCUs, would represent the safest approach to conserve species extant genetic variation in this part of the species range. Nonetheless, a strategy to prioritize populations for conservation based on their contribution to allelic diversity has been put forward. Given the rapid global warming and peculiarities of Serbian spruce distribution, habitat and life history traits, conservation measures based on a rigorously designed GCU network are urgent for its rescue and survival.
The increase in length and severity of drought events predicted for Southeast Europe are expected... more The increase in length and severity of drought events predicted for Southeast Europe are expected to engender important changes to its remaining native forest. To take informed management decision promoting their conservation, it is important to better understand their responses to environmental disturbances. In this study we analyze tree-ring growth responses over a network of 15 sites of Serbian spruce (Picea omorika), an endemic relict conifer species of the Balkan region-with a natural range restricted to the canyon of the Drina river at the border between Serbia and Bosnia Herzegovina-and that has already shown signs of decline and dieback likely induced by climate change. Tree-ring analyses spanning the common period from 1974 to 2016 have proved a strong growth reduction in the last 30 years and highlighted a negative growth response to summer drought, although the strength of the response differed among individuals, where younger trees and trees growing at lower altitude suffered more from drought. Management practice, such as inter-and intra-specific thinning to reduce competition for water resources and enhance survival of seedlings, together with assisted natural regeneration and migration to more suitable habitats are recommended for the conservation of this relict species. The measures are even more necessary considering that this species is more vulnerable than others due to its weak capacity to naturally regenerate and compete.
The paper presents the results of an analysis of morphometric characteristics of Norway spruce se... more The paper presents the results of an analysis of morphometric characteristics of Norway spruce seedlings in the progeny tests in Bosnia and Herzegovina, localities: Drinic and Srebrenica. Progeny tests were established by using seedlings originating from six natural populations from Bosnia and Herzegovina. Seedling height, root collar and whorl distance from the ground, which represents seedling heights in 2011, 2012 and 2013 were measured. The results indicate statistically significant differences between the analyzed characteristics of the observed population. Especially interesting are the populations Potoci and Olovo, which showed the highest growth during the observed period in both progeny tests. The impact of the drought in 2013 can be seen in the progeny test in Srebrenica where height growth in 2013 was lower by more than 50% in comparison to 2012. The impact of drought in the progeny test in Drinic was not observed. The research provides the baseline when selecting the sta...
Information on public attitudes towards the use of transgenic trees in forest plantations is impo... more Information on public attitudes towards the use of transgenic trees in forest plantations is important in the decision-making process and policy implementation for safe tree development, particularly at the EU level. In Europe, the use of transgenic forest trees is very limited and therefore such information is completely lacking. To address this issue within the FP0905 European COST Action on the Biosafety of Transgenic Forest Trees a pioneer cross-country pilot survey on public attitudes towards the use of transgenic forest trees was conducted using young population as a focus group. This was decided mainly because this focus group represents the future consumers, policy makers or developers. Specifically, the survey aimed to: i) assess the level of young people's knowledge about transgenic forest trees, ii) identify issues of concern to them regarding the cultivation of transgenic forest trees and iii) explore whether they approve or disapprove of the use of transgenic forest trees in plantations. Purposive sampling was performed and university students of different disciplines were included in the research as sampling subjects. In total, 1868 completed questionnaires from 15 European and non-European countries were analyzed. The young educated people that took part in the survey appeared to approve of the use of transgenic forest trees in plantations and would be willing to buy forest transgenic products. The potential loss of biodiversity due to a risk of gene flow between transgenic and wild trees was seen as the safety issue of most concern when considering the commercial release of transgenic forest trees. However, a serious perceived lack of knowledge about potential benefits and risks of the cultivation of transgenic forest trees was recorded in most of the countries. K-means clustering was implemented on respondents' positive responses to identify potential country patterns. No differences in patterns of public attitude towards the acceptance of the commercial growing of transgenic forest trees were observed between European and non-European countries. Extended research on public attitude issues towards the use of transgenic forest trees is strongly recommended as a basis for policy implementation on safe tree development.
одређивање специфичних приоритета: идентификација популација које ће обухватити процес конзерваци... more одређивање специфичних приоритета: идентификација популација које ће обухватити процес конзервације 6.6. In situ стратегије конзервације 6.7. организација и планирање специфичне активности у процесу конзервације 6.8. Припрема упустава за управљање објектима конзервације 6.9. избор и управљање областима за in situ конзервацију 6.9.1. број области за конзервацију 6.9.2. Величина сваке површине предвиђене за конзервацију 6.9.3. избор појединачних популација у оквиру сваке генеколошке зоне 6.9.4. израда плана управљања за сваки конзервацијску област 6.10. очувањe шумских генетичких ресурса у заштићеним подручима 6.10.1. јачање улоге заштићених подручја у процесу конзервације шумских генетичких ресурса 7. Ex SItU коНЗеРВацијa и коРиШћење ШУМСког геНеТичког ДиВеРЗиТеТа 7.1. Питања која треба узети у обзир приликом ex situ конзервације 7.2. Стратегија узорковања у процесу ex situ конзервације 7.3. оснивање и управљање ex situ конзервацијским засадима 7.3.1. циљ ex situ конзервације у засадима 7.3.2. Материјал и методе за успостављање ex situ конзервацијских засада 7.3.3. Планирање ex situ конзервационих засада 7.3.4. Успостављање ex situ засада 7.4. Ex situ конзервација путем складиштења и коришћења 7.4.1. еx situ конзервација путем складиштења сјемена 7.4.2. криопрезервација сјемена и клијанаца 7.4.3. In vitro конзервација 7.4.4. Складиштење полена 7.4.5. ДНк складиштење 7.5. опште препоруке за in situ и ex situ очување шумских генетичких ресурса 66 8. оДРжиВо гаЗДоВање ШУМаМа У ВеЗи ШУМСких геНеТичких РеСУРСа 8.1. Системи газдовања и очување шумских генетичких ресурса у различитим типовима шума 8.1.1. Усаглашавање сјеча са очувањем шумских генетичких ресурса 8.2. Праћење и оцјена утицај управљања на шумске генетичке ресурсе 8.3. Управљање осталим (недрвним) шумским производима 8.4. оплемењивање и очување шумских генетичких ресурса 8.4.1. обезбеђивање потенцијала за коришћење у будућности 9. иСТРажиВање и РаЗВој 9.1. Значај специфичних студија за програме практичне конзервације 9.2. гдје се налазе преостале популације, и који је статус конзервације одабраних врста? 9.3. истраживачки рад који треба да помогне у доношењу одлука о конзервацији популација 9.4. колико велика јединица конзервације треба да буде? 9.5. информације и истраживачки рад који потпомажу развој смјерница за управљање и коришћење конзервираних популација 9.6. Примјена молекуларних истраживања у процесу очувања генетичких ресурса 9.7. гдје ставити акценат у истраживачком раду?
Metadata Excel sheets of the data published under the title of "A genomic data set of single... more Metadata Excel sheets of the data published under the title of "A genomic data set of single‐nucleotide polymorphisms (SNPs) generated by RAD tag sequencing in <em>Q. petraea</em> (Matt.) Liebl. populations from Central-Eastern Europe and Balkan Peninsula"
Ex situ conservation is the preservation of components of biological diversity outside their natu... more Ex situ conservation is the preservation of components of biological diversity outside their natural habitats. Such ex situ work is an increasingly important component of conservation and restoration efforts. Its primary objective is to support conservation by ensuring the survival of threatened species and the maintenance of associated genetic diversity. To do so, ex situ institutions preserve the genetic or reproductive material of target species, or take care of the living target species for the purpose of reintroduction. Ex situ conservation measures should support in situ conservation measures. Organized ex situ conservation activities in Bosnia and Herzegovina (BiH) started in the middle of the last century. Activities were stopped during the 1990s because of the civil war, and then after 2000, the ex situ conservation of species continued. Ex situ cultures in BiH are established within arboreta, live archives, provenance tests, progeny tests, seed orchards and clone tests.
The total area under protection on the territory of Bosnia and Herzegovina is around 2% with tend... more The total area under protection on the territory of Bosnia and Herzegovina is around 2% with tendency to increase. The conservation of forest ecosystems “in situ” in Bosnia and Herzegovina is comprises: natural reserves, national parks, groups of trees or individual trees and seed stands. There are 2 Strict Nature Reserves, 4 National Parks, 16 Natural Monuments, 5 Protected Landscape and 2 protected areas with sustainable use of natural resources. The total area of delineated forest seed sources is 2154.93 ha 212 which consist of 121 coniferous and 88 deciduous species. The management in all in-situ areas is prescribed by law and rulebooks.
The paper presents the results of investigation of variability of net photosynthesis (A), transpi... more The paper presents the results of investigation of variability of net photosynthesis (A), transpiration (E), stomatal conductance (gs) and water use efficiency (WUE) in one year old seedlings of wild cherry (Prunus avium L.), assessed in a nursery trial. Study involved 5 families of half-sib progenies which originate from the Northern part of Serbia (Vojvodina Province). The results showed significant differences among families in regards of stomatal con-ductance (0.163-0.256 mol m-2 s-1), transpiration (3.27-5.28 mmol m-2 s-1) and water use efficiency (1.98-3.80 μmol mmol −1) (p≤0.001), while differences regarding net photosynthesis (10.49-12.44 μmol m-2 s-1) were not statistically significant (p≤0.124). Canonical discriminant analysis (CDA) was performed in order to estimate mul-tivariate relations among analyzed leaf photosyntetical traits. Families were separated by the first canonical axis (CD1), which described 82% of variability. Presence of differences in regards of E, gs an...
This paper presents the results of studies on protein polymorphism in seeds of Scots pine (Pinus ... more This paper presents the results of studies on protein polymorphism in seeds of Scots pine (Pinus sylvestris L.) and Austrian pine (Pinus nigra Arn.) as the most important economic species of the genus Pinus in Serbia. Polymorphism of protein markers was determined in selected genotypes originating from seven populations (Scots pine) and six populations (Austrian pine). Analysis of protein markers was performed using two statistical methods, NTSYS and correspondence analysis. Both methods give the same arrangement of the analyzed populations, whereby, because of a different view of genetic distances, they can and should be combined, 478
dynamics of germination and morphometrics properties of Austrian pine (Pinus nigra Arnold) saplin... more dynamics of germination and morphometrics properties of Austrian pine (Pinus nigra Arnold) saplings in terms of early indicators of tolerance toward the drought- Genetika, Vol 43, No. 1,75-90. Genetic markers, from morphological to molecular, in function with early indicators of tolerance toward drought, have been an object of research and scientific papers for many years. It starts with the hypothesis that seedlings produced from seeds that were collected from population of extremely different site conditions, on the level of open pollinated families, will have different results concerning drought tolerance. By tracking the 76 GENETIKA, Vol. 43, No. 1, 75-90, 2011 dynamics of germination and morphological parameters of saplings, we are researching the interdependence of these parameters with the survival and growth of two-year-old seedlings in conditions where there is a lack of water. Austrian Pine seeds were collected from forty trees, distributed over five provenances on the Bal...
According to the data obtained after the Second National Forest Inventory in Bosnia and Herzegovi... more According to the data obtained after the Second National Forest Inventory in Bosnia and Herzegovina (2006–2009) was carried out, the total area of forests and forest land in Bosnia and Herzegovina (BiH) is 3,231,500 ha or 63.08%, whilst the area covered by forest takes up 2,904,600 ha, or 56.7% of the total Bosnia and Herzegovina land area The land area of BiH is divided into four ecological vegetation areas (Pripanon, Inner Dinarides, Transitional Illyrian-Moesiac area and Mediterranean area). The spatial distribution of the main vegetation types is influenced by horizontal zonation and altitudinal belts. These areas are then followed by the mountainous belt with beech forests, beech and fir, beech and fir with spruce and the subalpine beech forest belt. There is a great number of endemic species and habitats and relic locations – a terrestrial flora and fauna shelter, which are now preserved under specific paleo-climate conditions. BiH is one of the countries in Europe with the highest diversity of both, plants and animals species, which are endangered by climate change. The abundance of diversity is reflected in a large number of plant and animal species within the region (about 4500 higher plants, 600 taxa mosses and about 80 ferns, around 250 species of forest trees and shrubs). This is why the position of BiH is high in the list of European countries of a high biodiversity level. The variability has been investigated by molecular markers for the most important species. The variability is high and it is important for meeting climate changes. Afforestation trends in BiH indicate that the afforestation volume decreased. Establishihng of new forests are not at the satisfactory level due to the lack of financial resources and insufficient attention to the aforested areas.
The total area under protection on the territory of Bosnia and Herzegovina is around 2% with tend... more The total area under protection on the territory of Bosnia and Herzegovina is around 2% with tendency to increase. The conservation of forest ecosystems “in situ” in Bosnia and Herzegovina is comprises: natural reserves, national parks, groups of trees or individual trees and seed stands. There are 2 Strict Nature Reserves, 4 National Parks, 16 Natural Monuments, 5 Protected Landscape and 2 protected areas with sustainable use of natural resources. The total area of delineated forest seed sources is 2154.93 ha 212 which consist of 121 coniferous and 88 deciduous species. The management in all in-situ areas is prescribed by law and rulebooks.
Developing “climate smart forestry” (CSF) indicators in mountain forest regions requires collecti... more Developing “climate smart forestry” (CSF) indicators in mountain forest regions requires collection and evaluation of local data and their attributes. Genetic resources are listed among the core indicators for forest biological diversity. This study is a report on the evaluation of the standing genetic diversity within and across 12 pure beech stands (Fagus sylvatica L.) established within the CLIMO (CLImate Smart Forestry in MOuntain Regions) project, using nuclear microsatellite markers. The sampling sites were set along the species’ distribution range, including the Balkan region and extending towards the Iberian Peninsula. Cores or leaves from 20 to 23 old, mature trees per plot were sampled for DNA analysis. Genetic diversity indices were high across the range (HE = 0.74−0.81) with the highest in the Bosnian Mountains. Genetic divergence increased significantly with the geographical distance (Mantel test: r = 0.81, p < 0.001). Most of the stands exhibited an excess of hetero...
ГЛАСНИК ШУМАРСКОГ ФАКУЛТЕТА УНИВЕРЗИТЕТА У БАЊОЈ ЛУЦИ, 2017
Cilj rada je analiza perioda cvjetanja vrsta roda lipa (Tilia sp.)u toku jedne vegetacione sezone... more Cilj rada je analiza perioda cvjetanja vrsta roda lipa (Tilia sp.)u toku jedne vegetacione sezone, na području grada Banja Luke. Vrsteobuhvaćene ovim istraživanjem su: krupnolisna, sitnolisna i srebrnolisnalipa. Terensko istraživanje je sprovedeno na tri lokaliteta: u parku MladenStojanović, Univerzitetskom gradu i na Starčevici. Utvrđene su klimatske,edafske i orografske karakteristike objekta istraživanja. Navedenilokaliteti, iako se nalaze relativno blizu u istom klimatskom području,pružaju različite ekološke uslove za razvoj i fenologiju istraživanih vrsta.Praćeno je pet faza cvjetanja, s ciljem da se utvrdi uticaj stanišnih uslovana fenofazu cvjetanja, te utvrde genetičke različitosti analiziranih stabala.Rezultati istraživanja pokazuju da početak i trajanje pojedinih fazacvjetanja istraživanih vrsta, u najvećoj mjeri zavise od vrste i stanišnihuslova. Na osnovu izvršenih fenoloških osmatranja konstatovane surazlike u trajanju perioda cvjetanja kod analiziranih stabala unutar i...
ГЛАСНИК ШУМАРСКОГ ФАКУЛТЕТА УНИВЕРЗИТЕТА У БАЊОЈ ЛУЦИ, 2020
Analiziranje kvaliteta sjemena i njegovog klijanja ima veliku važnost u proučavanju varijabilitet... more Analiziranje kvaliteta sjemena i njegovog klijanja ima veliku važnost u proučavanju varijabiliteta pitomog kestena. Ovim analizama se u najranijem uzrastu biljaka može utvrditi obim potencijalne genetičke varijabilnosti vrste. U radu su prikazani rezultati ispitivanja kvaliteta sjemena, direktnim (tehnička klijavost) i indirektnim metodom (ispitivanje vitaliteta embriona pomoću Tetrazolijum testa – TZ-test), za uzorke pitomog kestena iz šest prirodnih populacija sa područja Bosne i Hercegovine (BiH). Ispitivanja su obavljena prema uputstvima „The International Seed Testing Association”. Analizom su obuhvaćene uzastopno dvije kalendarske godine. Utvrđena je niska prosječna vrijednost klijavosti sjemena kao i vitaliteta embriona za sve istraživane populacije u obje godine istraživanja. Poznavanje kvaliteta sjemena važan je faktor za sve koji su uključeni u proces proizvodnje sjemena, a informacija o njegovom kvalitetu omogućava smjernice tokom proizvodnje, trgovine i skladištenja.
The article was published without proper open access tagging on SpringerLink. The original articl... more The article was published without proper open access tagging on SpringerLink. The original article has been corrected. Acknowledgements This designation is without prejudice to positions on status, and is in line with UNSCR 1244 (1999) and the ICJ Opinion's on the Kosovo* Declaration of Independence.
& Key message High levels of genetic diversity, pronounced genetic structure and limitations to g... more & Key message High levels of genetic diversity, pronounced genetic structure and limitations to gene flow in Serbian spruce, a rare and endangered tree species from the refugial Balkan region, point towards a "one population-one unit" strategy for assembling a network of Genetic Conservation Units (GCUs) for its dynamic conservation. On the other hand, genetic information also permits to prioritize populations for conservation based on their contribution to genetic diversity and differentiation. & Context Serbian spruce, Picea omorika (Panč.) Purk., is a rare, IUCN red-listed European conifer endemic to the Balkan region. Its current rigid conservation (without any intervention allowed in~30 remnant populations) and the extant network of Genetic Conservation Units (four natural populations and three planted stands from the western part of the species range, in the Republic of Srpska, Bosnia and Herzegovina, RS-BH) might be ineffective in preserving the species' genetic diversity. & Aims To facilitate implementation of dynamic conservation of Serbian spruce by reassessing the number and size of remnant populations in RS-BH and updating genetic knowledge on these understudied western Serbian spruce populations. & Methods Comprehensive field survey in RS-BH, genotyping 689 individuals from 14 western populations with ten highly informative nuclear EST-SSRs and analytical methods for prioritizing populations for conservation based on their contribution to the geographical structuring of genetic diversity. & Results The genetic diversity of western Serbian spruce populations (Ae = 2.524, H E = 0.451) is comparable with what was found for eastern ones; they are highly genetically differentiated (Hedrick's G' ST = 0.186; Jost's D = 0.097) and comprise ten distinct gene pools. Effective population size is often ≥ 15. As much as 14% of alleles is not preserved in the extant GCUs established in natural populations. Eight populations positively contribute to within-population genetic diversity, four to genetic differentiation, and two are globally important in terms of diversity and differentiation. Although wildfires may contribute to admixture of different gene pools, re-establishment from seeds from extirpated populations has likely prevailed in studied populations. & Conclusions A larger network of GCUs is required for the dynamic conservation of western Serbian spruce populations. A "one population-one unit" strategy, with 14 GCUs, would represent the safest approach to conserve species extant genetic variation in this part of the species range. Nonetheless, a strategy to prioritize populations for conservation based on their contribution to allelic diversity has been put forward. Given the rapid global warming and peculiarities of Serbian spruce distribution, habitat and life history traits, conservation measures based on a rigorously designed GCU network are urgent for its rescue and survival.
The increase in length and severity of drought events predicted for Southeast Europe are expected... more The increase in length and severity of drought events predicted for Southeast Europe are expected to engender important changes to its remaining native forest. To take informed management decision promoting their conservation, it is important to better understand their responses to environmental disturbances. In this study we analyze tree-ring growth responses over a network of 15 sites of Serbian spruce (Picea omorika), an endemic relict conifer species of the Balkan region-with a natural range restricted to the canyon of the Drina river at the border between Serbia and Bosnia Herzegovina-and that has already shown signs of decline and dieback likely induced by climate change. Tree-ring analyses spanning the common period from 1974 to 2016 have proved a strong growth reduction in the last 30 years and highlighted a negative growth response to summer drought, although the strength of the response differed among individuals, where younger trees and trees growing at lower altitude suffered more from drought. Management practice, such as inter-and intra-specific thinning to reduce competition for water resources and enhance survival of seedlings, together with assisted natural regeneration and migration to more suitable habitats are recommended for the conservation of this relict species. The measures are even more necessary considering that this species is more vulnerable than others due to its weak capacity to naturally regenerate and compete.
The paper presents the results of an analysis of morphometric characteristics of Norway spruce se... more The paper presents the results of an analysis of morphometric characteristics of Norway spruce seedlings in the progeny tests in Bosnia and Herzegovina, localities: Drinic and Srebrenica. Progeny tests were established by using seedlings originating from six natural populations from Bosnia and Herzegovina. Seedling height, root collar and whorl distance from the ground, which represents seedling heights in 2011, 2012 and 2013 were measured. The results indicate statistically significant differences between the analyzed characteristics of the observed population. Especially interesting are the populations Potoci and Olovo, which showed the highest growth during the observed period in both progeny tests. The impact of the drought in 2013 can be seen in the progeny test in Srebrenica where height growth in 2013 was lower by more than 50% in comparison to 2012. The impact of drought in the progeny test in Drinic was not observed. The research provides the baseline when selecting the sta...
Information on public attitudes towards the use of transgenic trees in forest plantations is impo... more Information on public attitudes towards the use of transgenic trees in forest plantations is important in the decision-making process and policy implementation for safe tree development, particularly at the EU level. In Europe, the use of transgenic forest trees is very limited and therefore such information is completely lacking. To address this issue within the FP0905 European COST Action on the Biosafety of Transgenic Forest Trees a pioneer cross-country pilot survey on public attitudes towards the use of transgenic forest trees was conducted using young population as a focus group. This was decided mainly because this focus group represents the future consumers, policy makers or developers. Specifically, the survey aimed to: i) assess the level of young people's knowledge about transgenic forest trees, ii) identify issues of concern to them regarding the cultivation of transgenic forest trees and iii) explore whether they approve or disapprove of the use of transgenic forest trees in plantations. Purposive sampling was performed and university students of different disciplines were included in the research as sampling subjects. In total, 1868 completed questionnaires from 15 European and non-European countries were analyzed. The young educated people that took part in the survey appeared to approve of the use of transgenic forest trees in plantations and would be willing to buy forest transgenic products. The potential loss of biodiversity due to a risk of gene flow between transgenic and wild trees was seen as the safety issue of most concern when considering the commercial release of transgenic forest trees. However, a serious perceived lack of knowledge about potential benefits and risks of the cultivation of transgenic forest trees was recorded in most of the countries. K-means clustering was implemented on respondents' positive responses to identify potential country patterns. No differences in patterns of public attitude towards the acceptance of the commercial growing of transgenic forest trees were observed between European and non-European countries. Extended research on public attitude issues towards the use of transgenic forest trees is strongly recommended as a basis for policy implementation on safe tree development.
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