Papers by Marjorie Layosa
The Journal of Nutritional Biochemistry
This study investigated in vivo the antitumor activity of dark sweet cherry (DSC) whole extracted... more This study investigated in vivo the antitumor activity of dark sweet cherry (DSC) whole extracted phenolics (WE) and fractions enriched in anthocyanins (ACN) or proanthocyanidins (PCA) in athymic mice xenografted with MDA-MB-453 breast cancer cells. Mice were gavaged with WE, ACN or PCA extracts (150 mg/kg body weight/day) for 36 days. Results showed that tumor growth was suppressed at similar levels by WE, ACN and PCA compared to control group (C) without signs of toxicity or significant changes in mRNA oncogenic biomarkers in tumors or mRNA invasive biomarker in distant organs. Tumor protein analyses showed that WE, ACN and PCA induced at similar levels the stress-regulated ERK1/2 phosphorylation, known to be linked to apoptosis induction. However, ACN showed enhanced antitumor activity through down-regulation of total oncogenic and stress-related Akt, STAT3, p38, JNK and NF-kB proteins. In addition, immunohistochemistry analysis of Ki-67 revealed inhibition of tumor cell proliferation with potency WE ≥ ACN ≥ PCA. Differential quantitative proteomic high-resolution nano-HPLC tandem mass spectrometry analysis of tumors from ACN and C groups revealed the identity of 66 proteins associated with poor breast cancer prognosis that were expressed only in C group (61 proteins) or differentially up-regulated (P<.05) in C group (5 proteins). These findings revealed ACN-targeted proteins associated to tumor growth and invasion and the potential of DSC ACN for breast cancer treatment. Results lead to a follow-up study with highly immunodeficient mice/invasive cell line subtype and advanced tumor development to validate the anti-invasive activity of DSC anthocyanins.
Journal of Functional Foods
Los videojuegos modelan las formas contemporáneas del ocio y la diversión. Pero también invadiero... more Los videojuegos modelan las formas contemporáneas del ocio y la diversión. Pero también invadieron otros ámbitos de la vida socio-cultural y colonizaron espacios tradicionalmente contrapuestos al entretenimiento. Hoy los juegos digitales mediatizan procesos de aprendizaje, tratamientos de rehabilitación virtual, entrenamientos complejos en situaciones de simulación. También lideran el nicho del mercado de las industrias culturales a nivel global en sus diferentes vertientes (el advergaming, los casual games, los serious games). El impacto en la conducta social exige técnicos y profesionales con capacitación creciente para responder a las
Current Developments in Nutrition
Objectives Dark sweet cherry (DSC) contains a rich mixture of phenolics reported to exert anti-ca... more Objectives Dark sweet cherry (DSC) contains a rich mixture of phenolics reported to exert anti-cancer and anti-metastasis activities. We aimed to investigate the underlying mechanisms by which DSC phenolics in whole extracts (WE) and fractions enriched in anthocyanins (ACN) or procyanidins (PCN) decrease proliferation and invasion on the HER2 + breast cancer MDA-MB-453 cells. Methods MDA-MB-453 cells were treated with DSC phenolics in WE, ACN, and PCN obtained by solid phase extraction (SPE) at doses that inhibited cell growth by 50% for 24 h. Protein expression over 24 h was assessed by western blots and invasion and metastasis potential were assessed with cell invasion and cell motility assays. Results DSC phenolics in WE, ACN, and PCN targeted intrinsic and extrinsic apoptotic pathways as shown by caspase-8 cleavage and cytochrome c/bax upregulation that culminate in caspase-9, -3, and PARP cleavage. In addition, a sustained activation of mitogen activated protein kinases (MAPKs)...
Current Developments in Nutrition
Objectives Dark sweet cherries (DSC) (Prunus avium) contain phenolic compounds with chemopreventi... more Objectives Dark sweet cherries (DSC) (Prunus avium) contain phenolic compounds with chemopreventive and chemotherapeutic potential. This study aimed to evaluate the effect of DSC extracts in inhibiting the most aggressive breast cancer subtypes without toxicity to normal cells. Methods DSC phenolic extracts (WE) or its fractions enriched in phenolic acids (F1), anthocyanins (F2), flavonols (F3) or procyanidins (F4) were assessed for their antiproliferative activity against the most aggressive breast cancer BT-474, MDA-MB-453, and MDA-MB-231 cell lines, and MCF-10A noncancer breast epithelial cells using the resazurin assay. Cell levels of oxidative stress were assessed with fluorescein derivative H2DFFDA. Expression of genes linked to cell growth, apoptosis, and metastasis was assessed. Results DSC phenolics in whole extract (WE) and fractions enriched in phenolic acids (F1), flavonols (F3) and procyanidins (F4) inhibited MDA-MB-453 with higher potency (lower IC50values) compared to...
Dark sweet cherries (DSCs) are rich source of phenolics known to exert anticancer and anti-invasi... more Dark sweet cherries (DSCs) are rich source of phenolics known to exert anticancer and anti-invasive activities. This study elucidated the molecular mechanisms underlying the activity of DSC phenolics against MDA-MB-453 breast cancer cells In Vitro. Cells were treated with DSC phenolics in whole extract (WE), and fractions enriched in anthocyanins (ACN) and proanthocyanidins (PCN) at concentrations that inhibited cell growth by 50%. Results showed that DSC phenolics suppressed Akt and PLCγ-1 activation, and inhibited cell motility and invasion, but only ACN reached significance. The extrinsic and intrinsic apoptotic pathways were also activated by DSC phenolics via caspase-8 cleavage and increased Bax/Bcl-2 ratio, with ACN exhibiting significant activation and stronger PARP-1 cleavage. Furthermore, sustained activation of mitogen-activated protein kinases (MAPKs) ERK1/2 and p38 was observed wherein ERK1/2 (U0126) and p38 (SB203580) inhibitors confirmed crosstalk ERK1/2-Akt and MAPK i...
Introduction: Brown rice is promoted for a healthier rice-consuming population as it renders nume... more Introduction: Brown rice is promoted for a healthier rice-consuming population as it renders numerous nutritional benefits due to its fiber and germ, yet may contain high concentrations of metal elements from environmental effluents. The purpose of this study is to identify the potential health risk of brown rice cultivated in different major islands in the Philippines. Methods: Concentrations of heavy metals cadmium (Cd) and lead (Pb) were investigated on brown rice of a popular modern rice variety (NSIC Rc222) cultivated from top rice-producing provinces in Luzon, Visayas and Mindanao, namely Nueva Ecija, Iloilo and Bukidnon, respectively, through nonprobability sampling. Total Hazard Quotient (THQ) and Combined Total Hazard Quotient (CTHQ), as developed by US EPA, were used to calculate the potential hazard. Results: Cd levels of brown rice from different sites were found to be below the maximum level of 0.1 mg/kg. However, Pb content from all sites exceeds the 0.2 mg/kg allowabl...
Journal of Medicinal Food
Jaboticaba (Myrciaria cauliflora), a Brazilian fruit, is a good source of dietary fiber and pheno... more Jaboticaba (Myrciaria cauliflora), a Brazilian fruit, is a good source of dietary fiber and phenolic compounds, which are concentrated mainly in the peel. These compounds have been considered promising in prevention and treatment of hypercholesterolemia and hepatic steatosis. In this study, we investigated the effects of 4% jaboticaba peel powder (JPP) supplementation on cholesterol metabolism and hepatic steatosis in livers of rats fed a high-fat (HF) diet. The rats were fed a standard AIN-93M (control) diet or an HF diet containing 32% lard and 1% cholesterol, both with and without 4% JPP. The M. cauliflora peel composition revealed a low-lipid high-fiber content and phenolic compounds. The phenolic compounds in JPP, tentatively identified by high-performance liquid chromatography and mass spectrometry (HPLC-MS/MS) analysis, were confirmed to contain phenolic acids, flavonoids, and anthocyanins. Moreover, JPP presented significant antioxidant activity in vitro and was not cytotoxic to HepG2 cells, as determined by the lactate dehydrogenase (LDH) assay. After 6 weeks of treatment, our results showed that JPP supplementation increased lipid excretion in feces, reduced serum levels of total cholesterol and nonhigh-density lipoprotein cholesterol, decreased serum aspartate aminotransferase (AST) activity, and attenuated hepatic steatosis severity in rats fed the HF diet. Furthermore, JPP treatment downregulated expression of ACAT-1, LXR-α, CYP7A1, and ABCG5 genes. Therefore, jaboticaba peel may represent a viable dietary strategy to prevent nonalcoholic fatty liver disease as the JPP treatment alleviated hepatic steatosis through improvement of serum lipid profiles and modulation of mRNA expression of genes involved in cholesterol metabolism.
Uploads
Papers by Marjorie Layosa