Papers by Maria Söderlund-Venermo
We identified a new genotype of bufavirus, BuV3, in fecal samples (0.8%) collected to determine t... more We identified a new genotype of bufavirus, BuV3, in fecal samples (0.8%) collected to determine the etiology of diarrhea in children in Bhutan. Norovirus GII.6 was detected in 1 sample; no other viral diarrheal pathogens were de-tected, suggesting BuV3 as a cause of diarrhea. This study investigates genetic diversity of circulating BuVs. In 2012, a novel parvovirus, bufavirus (BuV), was dis-covered in fecal samples of children with diarrhea in Burkina Faso (1). The virus belongs to the species primate protoparvovirus 1 of the genus Protoparvovirus (2). BuV has a single-stranded DNA genome and encodes nonstruc-tural protein 1 (NS1) and viral structural proteins 1 and 2 (VP1 and VP2). Two genotypes, BuV1 and BuV2, have been described; the highly diverse capsid gene indicates the possibility of further genotypes of this virus (1). One research group, which used PCR to test fecal samples collected in 3 countries, had previously found various proportions of specimens positive for
International Journal of Antimicrobial Agents, 2013
Objectives: To determine the prevalence and character of bacterial contamination, and incidence o... more Objectives: To determine the prevalence and character of bacterial contamination, and incidence of wound infection in adult patients with fresh traumatic wounds attending Siriraj Trauma Center. Methods: Prospective study was conducted in 330 adult patients with fresh traumatic wounds during March 2012 and July 2012 at Siriraj Trauma Center. The eligible patients received wound care and antibiotic prophylaxis according to judgments of their responsible physicians. Wound swab cultures were taken from all patients. The clinical data from all patients as well as microbiological results from wound cultures were collected and analyzed by descriptive statistics. Results: Ninety-one percent of them received antibiotics in which dicloxacillin and co-amoxiclav accounted for 73% and 10%, respectively. Wound swab cultures revealed that potential pathogenic bacteria i.e. S. aureus , streptococci, Enterobacteriaceae, Aeromonas spp., Acinetobacter spp. and non-fermentative Gram-negative rods were recovered from 7% of wounds. Incidence of wound infection was 1.2%, and all infected wounds were found in patients who received antibiotic prophylaxis. Conclusion: More than 90% of adult patients with fresh traumatic wound at Siriraj Trauma Center received prophylactic antibiotics. Less than 10% of these wounds were contaminated with potentially pathogenic bacteria. Incidence of wound infection in fresh traumatic wounds was very low.
Clinical Microbiology and Infection, Oct 1, 2015
Recently a parvovirus called bufavirus (BuV) has been implicated as a causative agent of diarrhoe... more Recently a parvovirus called bufavirus (BuV) has been implicated as a causative agent of diarrhoea. To further reveal the epidemiology and genetic characteristics of BuV, this study was performed in Turkish children with diarrhoea. BuV was detected in 1.4% (8/583) of stool samples. All stool samples from healthy children (n = 148) were negative for BuV. Diarrhoea in BuV-positive patients was severe and occurred mainly during the colder months of the year. Complete genome sequences were generated from four BuVs. Only BuV3 was found, which was genetically and phylogenetically similar to Bhutanese BuV3, indicating that BuV3 is prevalent in Asian countries.
The Lancet Child & Adolescent Health, Jun 1, 2019
Human bocavirus 1 (HBoV1), belonging to the Parvoviridae family, was discovered in 2005, in nasop... more Human bocavirus 1 (HBoV1), belonging to the Parvoviridae family, was discovered in 2005, in nasopharyngeal samples from children with respiratory tract infections. Three additional bocaviruses, HBoV2-4, were discovered in 2009-10. These viruses have mainly been found in faecal samples and their role in human diseases is still uncertain. HBoV1 causes a wide spectrum of respiratory diseases in children, including common cold, acute otitis media, pneumonia, bronchiolitis, and asthma exacerbations. HBoV1 DNA can persist in airway secretions for months after an acute infection. Consequently, acute HBoV1 infection cannot be diagnosed with standard DNA PCR; quantitative PCR and serology are better diagnostic approaches. Because of their high clinical specificity, diagnostic developments such as HBoV1 mRNA and antigen detection have shown promising results. This Review summarises the knowledge on human bocaviruses, with a special focus on HBoV1.
Journal of General Virology, Mar 1, 2019
Members of the family Parvoviridae are small, resilient, non-enveloped viruses with linear, singl... more Members of the family Parvoviridae are small, resilient, non-enveloped viruses with linear, single-stranded DNA genomes of 4-6 kb. Viruses in two subfamilies, the Parvovirinae and Densovirinae, are distinguished primarily by their respective ability to infect vertebrates (including humans) versus invertebrates. Being genetically limited, most parvoviruses require actively dividing host cells and are host and/or tissue specific. Some cause diseases, which range from subclinical to lethal. A few require co-infection with helper viruses from other families. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the Parvoviridae, which is available at www.ictv.global/report/parvoviridae.
British Journal of Dermatology, Oct 1, 2017
comparable with the average patient with psoriasis than those from hospital-based studies. Unfort... more comparable with the average patient with psoriasis than those from hospital-based studies. Unfortunately, the study lacked a nonpsoriasis control group, and the prevalence of different sleep patterns in psoriasis may thus be difficult to compare with that from the general population. Although the topic of sleep disturbances has already been extensively described, the present study may direct clinicians' attention to where therapeutic efforts should be put in order to improve patient-reported outcomes when managing psoriasis.
Allergy, Oct 8, 2018
Background: Wheezing illnesses among young children are common and are a risk factor for asthma. ... more Background: Wheezing illnesses among young children are common and are a risk factor for asthma. However, determinants of childhood bronchial reactivity, a key feature of asthma, are largely unknown. The aim of this study was to determine how patient characteristics during the first severe virus-induced wheezing episode are associated with pulmonary function at preschool age. Methods: Study consisted of 76 children presenting with their first wheezing episode at the ages of 3 to 23 months. At study entry, viral etiology, rhinovirus genome load, atopic and clinical characteristics, and standardized questionnaire were analyzed. At 4-year follow-up visit, impulse oscillometry with exercise challenge was performed. Results: At study entry, the mean age of the children was 12 months (SD 6.0), 57 (75%) were rhinovirus positive, and 22 (30%) were sensitized. At follow-up visit four years later, the mean age of the children was 60 months (SD 7.9) and 37 (49%) were using asthma medication regularly (discontinued before testing in 25 [68%] children). Bronchial reactivity (≥35% change in mean crude values of resistance) after exercise challenge or bronchodilation was present in nine (12%) children. Children with atopic sensitization at the time of the first wheezing episode were more often likely to develop bronchial reactivity (odds ratio 8.8, P = 0.03
Frontiers in Microbiology, May 15, 2023
PLOS ONE, Sep 29, 2015
<p>Human bocavirus 1–3 seroprevalences (dashed lines) and cumulative seroconversion rates (... more <p>Human bocavirus 1–3 seroprevalences (dashed lines) and cumulative seroconversion rates (solid lines) in different age groups.</p
Viruses, Feb 20, 2021
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
The Open Respiratory Medicine Journal, Jul 6, 2011
The diagnostics of respiratory viral infections has improved markedly during the last 15 years wi... more The diagnostics of respiratory viral infections has improved markedly during the last 15 years with the development of PCR techniques. Since 1997, several new respiratory viruses and their subgroups have been discovered: influenza A viruses H5N1 and H1N1, human metapneumovirus, coronaviruses SARS, NL63 and HKU1, human bocavirus, human rhinoviruses C and D and potential respiratory pathogens, the KI and WU polyomaviruses and the torque teno virus. The detection of previously known viruses has also improved. Currently, a viral cause of respiratory illness is almost exclusively identifiable in children, but in the elderly, the detection rates of a viral etiology are below 40%, and this holds also true for exacerbations of chronic respiratory illnesses. The new viruses cause respiratory symptoms like the common cold, cough, bronchitis, bronchiolitis, exacerbations of asthma and chronic obstructive pulmonary disease and pneumonia. Acute respiratory failure may occur. These viruses are distributed throughout the globe and affect people of all ages. Data regarding these viruses and the elderly are scarce. This review introduces these new viruses and reviews their clinical significance, especially with regard to the elderly population.
Frontiers in Microbiology, May 24, 2022
Parvoviruses are single-stranded DNA viruses, infecting many animals from insects to humans. Huma... more Parvoviruses are single-stranded DNA viruses, infecting many animals from insects to humans. Human parvovirus B19 (B19V) causes erythema infectiosum, arthropathy, anemia, and fetal death, and human bocavirus (HBoV) 1 causes respiratory tract infections, while HBoV2-4 are enteric. Parvoviral genomes can persist in diverse non-permissive tissues after acute infection, but the host-cell tropism and the impact of their tissue persistence are poorly studied. We searched for parvoviral DNA in a total of 427 intestinal biopsy specimens, as paired disease-affected and healthy mucosa, obtained from 130 patients with malignancy, ulcerative colitis (UC), or adenomas, and in similar intestinal segments from 55 healthy subjects. Only three (1.6%) individuals exhibited intestinal HBoV DNA (one each of HBoV1, 2, and 3). Conversely, B19V DNA persisted frequently in the intestine, with 50, 47, 31, and 27% detection rates in the patients with malignancy, UC, or adenomas, and in the healthy subjects, respectively. Intra-individually, B19V DNA persisted significantly more often in the healthy intestinal segments than in the inflamed colons of UC patients. The highest loads of B19V DNA were seen in the ileum and colon specimens of two healthy individuals. With dual-RNAscope in situ hybridization and immunohistochemistry assays, we located the B19V persistence sites of these intestines in mucosal B cells of lymphoid follicles and vascular endothelial cells. Viral messenger RNA transcription remained, however, undetected. RNA sequencing (RNA-seq) identified 272 differentially expressed cellular genes between B19V DNA-positive and-negative healthy ileum biopsy specimens. Pathway enrichment analysis revealed that B19V persistence activated the intestinal cell viability and inhibited apoptosis. Lifelong B19V DNA persistence thus modulates host gene expression, which may lead to clinical outcomes.
Viruses, Jan 4, 2018
Bufavirus strain 1 (BuV1), a member of the Protoparvovirus genus of the Parvoviridae, was first i... more Bufavirus strain 1 (BuV1), a member of the Protoparvovirus genus of the Parvoviridae, was first isolated from fecal samples of children with acute diarrhea in Burkina Faso. Since this initial discovery, BuVs have been isolated in several countries, including Finland, the Netherlands, and Bhutan, in pediatric patients exhibiting similar symptoms. Towards their characterization, the structures of virus-like particles of BuV1, BuV2, and BuV3, the current known genotypes, have been determined by cryo-electron microscopy and image reconstruction to 2.84, 3.79, and 3.25 Å, respectively. The BuVs, 65-73% identical in amino acid sequence, conserve the major viral protein, VP2, structure and general capsid surface features of parvoviruses. These include a core β-barrel (βB-βI), α-helix A, and large surface loops inserted between these elements in VP2. The capsid contains depressions at the icosahedral 2-fold and around the 5-fold axes, and has three separated protrusions surrounding the 3-fold axes. Structure comparison among the BuVs and to available parvovirus structures revealed capsid surface variations and capsid 3-fold protrusions that depart from the single pinwheel arrangement of the animal protoparvoviruses. These structures provide a platform to begin the molecular characterization of these potentially pathogenic viruses.
Journal of Clinical Virology, Sep 1, 2016
Microscopy and Microanalysis, Aug 1, 2018
ASM Press eBooks, Mar 7, 2016
Annual Review of Virology, Sep 29, 2019
Parvoviruses are structurally simple viruses with linear single-stranded DNA genomes and nonenvel... more Parvoviruses are structurally simple viruses with linear single-stranded DNA genomes and nonenveloped icosahedral capsids. They infect a wide range of animals from insects to humans. Parvovirus B19 is a long-known human pathogen, whereas adeno-associated viruses are nonpathogenic. Since 2005, many parvoviruses have been discovered in human-derived samples: bocaviruses 1–4, parvovirus 4, bufavirus, tusavirus, and cutavirus. Some human parvoviruses have already been shown to cause disease during acute infection, some are associated with chronic diseases, and others still remain to be proven clinically relevant—or harmless commensals, a distinction not as apparent as it might seem. One initially human-labeled parvovirus might not even be a human virus, whereas another was originally overlooked due to inadequate diagnostics. The intention of this review is to follow the rocky road of emerging human parvoviruses from discovery of a DNA sequence to current and future clinical status, highlighting the perils along the way.
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Papers by Maria Söderlund-Venermo