The Louisiana Dept. of Agriculture and Forestry (LDAF) conducts sweetpotato weevil [SPW, Cylas fo... more The Louisiana Dept. of Agriculture and Forestry (LDAF) conducts sweetpotato weevil [SPW, Cylas formicarius ( Fabricius )] monitoring in support of the statewide SPW quarantine program. The monitoring activity primarily involves a statewide pheromone-based trapping process that generates trap data for sweetpotato beds and production fi elds. We conducted GIS analysis of SPW trap data, collected over three years, to assess the potential use of GIS tools in managing and interpreting the data. The LDAF has already generated shapefi les for all beds and fi elds in each of three years, facilitating GIS analysis. However, trap data was manually collected and statewide data was compiled and stored in spreadsheet fi les. Trap data was mapped to specifi c beds and fi elds in each of three years, generating layers that clearly showed fi elds and parishes that reported high trap counts. GIS analysis showed potential SPW "hotspots" in each year, indicating that certain beds or fi elds are more prone to SPW infestation than others. This information can be useful in planning SPW management strategies by growers and other stakeholders. The GIS database also provides the foundation for the development of descriptive and predictive models of SPW occurence in Louisiana. Compiling the SPW trap data into a GIS database allows the data to be distributed over the Internet, facilitating real-time access by stakeholders.
The United States of America collection of mountain mint (Pycnanthemum Michx.) is held at the USD... more The United States of America collection of mountain mint (Pycnanthemum Michx.) is held at the USDA-ARS National Clonal Germplasm Repository (NCGR) in Corvallis, Oregon as seed, potted plants and tissue cultures and a long-term storage collection is preserved at the USDA-ARS National Center for Genetic Resources Preservation (NCGRP) in Fort Collins, Colorado. The clonal collection is comprised of 34 accessions as potted plants that are duplicated with 31 accessions stored as in vitro cultures at 4 degrees C in tissue culture bags for medium-term storage at NCGR and as cryopreserved shoot tips in liquid nitrogen at NCGRP for long-term storage. This study reports on these two models of preservation of mountain mint at the U.S. National Plant Germplasm System. In vitro plants required 2 to 7 months for propagation on MS medium without growth regulators before storage at 4 degrees C. Plants remained in storage with good vigour in bags on 1/2x nitrogen MS medium without growth regulators for a mean of 2.08 y. An encapsulation-dehydration protocol was successful for cryopreservation of shoot tips from cold acclimated in vitro plants. Post-cryo viability, indicated by shoot tips with developed leaves and roots, ranged from 60 to 100 % for 27 accessions and 40 to 50 % for the other four. The encapsulation-dehydration cryopreservation method proved suitable for long-term preservation of the 31 Pycnanthemum accessions. These alternative storage forms allow for active use of the collection as well as base storage for clonally propagated accessions.
All rights reserved. No part of this periodical may be reproduced or transmitted in any form or b... more All rights reserved. No part of this periodical may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Permission for printing and for reprinting the material contained herein has been obtained by the publisher.
The genus Limnanthes (Limnanthaceae), also known as meadowfoam, has attracted attention for indus... more The genus Limnanthes (Limnanthaceae), also known as meadowfoam, has attracted attention for industrial use due to the unique characteristics of its seed oil. Samples from wild populations showed variability in agronomically important traits involved in seed oil yield, warranting the establishment and continued development of a germplasm collection. The level of genetic diversity within the United States Department of Agriculture, Agriculture Research Service, National Plant Germplasm System (USDA-ARS-NPGS) Limnanthes collection was evaluated using 15 simple sequence repeat (SSR) markers across 62 accessions representing 7 species. Parsimony analysis separated the accessions into two main groups consistent with the traditional taxonomic sections Inflexae and Reflexae, but there was little resolution within groups. These two groups were confirmed using neighbour-joining analysis and principal coordinate analysis. SSR marker variation suggests that the Limnanthes germplasm collection is genetically diverse and the accessions within the species likely contain novel alleles, and therefore the collection contributes to the conservation of the wild Limnanthes gene pool.
Secretion of phenolic compounds is a major limitation for sugarcane in vitro shoot culture, causi... more Secretion of phenolic compounds is a major limitation for sugarcane in vitro shoot culture, causing a loss of regenerative capacity and subsequent cell death. In this study, micropropagation and phenolic secretion of four Saccharum genotypes were evaluated in presence of different antioxidants. Aseptic cultures of S. officinarum (PI 184794 and PI 88652), S. sinense (PI 29109) and S. robustum (UNK R65P35) were propagated on medium containing antioxidants, citric acid (100 mg/L), L-cysteine (100 mg/L), polyvynylpirrolidone (300 mg/L) and L-glutathione (50 mg/L) in two consecutive subculture cycles. Interaction between genotypes and antioxidants was significant in both cycles. All genotypes showed good shoot formation, shoot vigor and color, except in PI 88652 which had less shoot development in both the presence and absence of the antioxidants tested. PI 184794 displayed the highest shoot proliferation in the presence of citric acid, and UNK R65P35 produced more shoots per explant in the 2 nd subculture. For S. sinense (PI 29109), in both subcultures, most shoots were observed in the presence of polyvynylpirrolidone. Medium discoloration due to phenolic secretion was reduced in the presence of citric acid and polyvynylpirrolidone. The type of secreted phenolic compounds differed with genotype as the Principal Component Analysis of cultivation media separated PI 88652 from PI 29109 and UKN R65P35. Phenolic compounds varied in composition and were secreted at various levels as a function of genotype and antioxidant type. Loadings plots indicated the genotype and antioxidant separations were broadly driven by flavonoid compounds.
Journal of the American Society for Horticultural Science, Jul 1, 2005
Garlic (Allium sativum L.) has been cultivated by asexual propagation since time immemorial. The ... more Garlic (Allium sativum L.) has been cultivated by asexual propagation since time immemorial. The discovery of male-fertile garlic accessions has opened a venue for genetic studies and improvement through sexual recombination. An S 1 family of 84 plants was generated from a single male-fertile heterozygous plant from the U.S. Dept. of Agriculture Plant Introduction 540316 and used to identify the fi rst genetic linkages in garlic based on single nucleotide polymorphisms, simple sequence repeats, and randomly amplifi ed polymorphic DNAs. Thirty-seven markers formed nine linkage groups covering 415 centimorgans (cM) with average distance of 15 cM between loci; other 16 loci remained unlinked. A male fertility locus was placed on the map. This fi rst genetic map of garlic is a seminal step toward the genetic improvement of garlic and eventual marker-assisted breeding. Garlic is an asexually propagated plant cultivated since time immemorial (Simon, 2004). The center of origin for garlic is central Asia, from which the plant spread worldwide and is now cultivated from mountainous tropical to temperate regions (Etoh and Simon, 2002). The bulb is the most economically important part of the garlic plant; however, its fresh leaves, pseudostems, and bulbils are also consumed by humans (Fritsch and Friesen, 2002). Although garlic is primarily consumed for its fl avor, it has well-defi ned medicinal benefi ts (Augusti, 1990; Block et al., 1984; Makheja and Bailey, 1990). The organosulfur compounds accumulated by garlic show signifi cant antibacterial, antifungal, anticarcinogenic, antiasthmatic, and antidiabetic properties, as well as lipid-lowering ability through inhibition of cholesterol biosynthesis (Keusgen, 2002). Commercial production of garlic is exclusively by asexual propagation. Sterility in garlic has been attributed to both genetic and environmental factors, including irregular chromosome pairing, chromosomal deletion, anther degeneration, and competition for nutrients between topsets and fl owers (Etoh and Simon, 2002). Because of asexual propagation, garlic improvement has been limited to clonal selection of spontaneous mutants or sports
Bulgaria is famous for its 330-year-old-tradition in rose oil production, which is based on the K... more Bulgaria is famous for its 330-year-old-tradition in rose oil production, which is based on the Kazanluk rose (Rosa damascena Mill. f. trigintipetala Dieck.). The Bulgarian rose oil (otto) is recognized as the ultimate rose oil. For successful selection and breeding work of oil-bearing roses, information is needed on the variation of morphological and phenological characteristics and essential oil composition of locally available genotypes. We estimated the correlation coefficients between yields and morphological characteristics of 15 genotypes of Bulgarian oil-bearing rose. It was found that rose yields depended mostly on the number of flowers, the number of flower branches per bush, and the weight of individual flowers (r = 0.99, 0.88, and 0.84, respectively). Also, we established correlations between the concentrations of various essential oil constituents of the Bulgarian rose oil. Generally, higher concentration of citronellol + nerol was associated with lower concentration of geraniol and stereo-terpens (r = -0.76 and -0.59, respectively). Also, higher concentration of citronellol + nerol was positively correlated to increased concentration of terpene aldehydes (r = 0.63) and esters (r = 0.48). The geraniol concentration was positively correlated to stearoptenes (r = 0.57). Both morphological characteristics and essential oil constituents should be used for further selection of high-yielding cultivars with desirable essential oil composition.
Its fl owers are predominantly allogamous; however, self-compatible plants may be found in the wi... more Its fl owers are predominantly allogamous; however, self-compatible plants may be found in the wild. Plants of this genus typically occupy riparian habitats and grow well in temperatures between 4 and 16 °C. Meadowfoam may therefore be considered as a winter-spring crop; however, extended periods of air temperatures below 5 °C are detrimental . Early agronomic studies indicated Limnanthes as suitable to be grown in California, Maryland, Oregon, and Alaska (Higgins et al., 1971).
Until recently, there has been no large-scale production of true seeds in garlic (A. sativum L. a... more Until recently, there has been no large-scale production of true seeds in garlic (A. sativum L. and A. longicuspis L.). The recent discovery of male fertile garlic accessions stimulated research on the genetics and breeding of garlic. However, there is no information regarding the phenotypic characteristics of garlic populations generated from true seeds. We evaluated the fi rst generation of sexually derived families of garlic for bulb and clove weight, number of cloves per bulb, fl ower stalk height, number of leaves, plant height, and days required to achieve bulb maturity. Signifi cant variations were observed within and among families for these important traits.
Enormous genomic resources have been developed for plants in the monocot order Poales; however, i... more Enormous genomic resources have been developed for plants in the monocot order Poales; however, it is not known how useful these resources will be for other economically important monocots. Asparagales are a monophyletic order sister to class Commelinanae that carries Poales, and is the second most economically important monocot order. Development of genomic resources for and their application to Asparagales are challenging because of huge nuclear genomes and the relatively long generation times required to develop segregating families. We synthesized a normalized eDNA library of onion (Allium cepa) and produced II ,008 unique expressed sequence tags (ESTs) for comparative genomic analyses of Asparagales and Poales. Alignments of onion ESTs, Poales ESTs, and genomic sequences from rice were used to design oligonucleotide primers amplifying genomic regions from asparagus, garlic, and onion. Sequence analyses of these genomic regions revealed microsatellites, insertions/deletions, and single nucleotide polymorphisms for comparative mapping of rice and Asparagales vegetables. Initial mapping revealed no obvious synteny at the recombinationallevel between onion and rice, indicating that genomic resources developed for Poales may not be applicable to the monocots as a whole. Genomic analyses of Asparagales would greatly benefit from EST sequencing and deep-coverage, large-insert genomic libraries of representative small-genome model species within the "higher" and "lower" Asparagales, such as asparagus and orchid, respectively.
Germanium has been reported as a mineral element affecting plant cell metabolism. Many trials to ... more Germanium has been reported as a mineral element affecting plant cell metabolism. Many trials to supply germanium to fruit have been carried out since tests have confi rmed germanium's role as a medical substance. Supplying germanium to orchards by soil and foliar application was not effective because of loss from rainfall. Also, tree injection with germanium solution required the insertion of a tube to the tree xylem at each injection site. In order to increase germanium absorption by fruit, this study carried out the postharvest dipping of fruit into germanium solution. 'Niitaka' pear ( Pyrus pyrifolia ) fruit was treated with two types of germanium, GeO (inorganic type) and Ge-132 (organic type), in a concentration of 50 mg•L -1 just after harvest in early Oct. 2004. Flesh browning after peeling the fruit was delayed by germanium treatment, and polyphenoloxidase (PPO) activities were lowered. Postharvest potentials were maintained at high levels for fruit fi rmness, physiological disorders, and decayed fruit during cold storage at 0 to 1 °C for 2 months. Antioxidant and some phenolic compounds were higher than those of control fruit.
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
The Louisiana Dept. of Agriculture and Forestry (LDAF) conducts sweetpotato weevil [SPW, Cylas fo... more The Louisiana Dept. of Agriculture and Forestry (LDAF) conducts sweetpotato weevil [SPW, Cylas formicarius (Fabricius)] monitoring in support of the statewide SPW quarantine program. The monitoring activity primarily involves a statewide pheromone-based trapping process that generates trap data for sweetpotato beds and production fi elds. We conducted GIS analysis of SPW trap data, collected over three years, to assess the potential use of GIS tools in managing and interpreting the data. The LDAF has already generated shapefi les for all beds and fi elds in each of three years, facilitating GIS analysis. However, trap data was manually collected and statewide data was compiled and stored in spreadsheet fi les. Trap data was mapped to specifi c beds and fi elds in each of three years, generating layers that clearly showed fi elds and parishes that reported high trap counts. GIS analysis showed potential SPW "hotspots" in each year, indicating that certain beds or fi elds are more prone to SPW infestation than others. This information can be useful in planning SPW management strategies by growers and other stakeholders. The GIS database also provides the foundation for the development of descriptive and predictive models of SPW occurence in Louisiana. Compiling the SPW trap data into a GIS database allows the data to be distributed over the Internet, facilitating real-time access by stakeholders. 2 Poster withdrawn.
In Vitro Cellular & Developmental Biology - Animal, 2007
... Goodacre, S. A-3003 Harrison, CA E-3000 Huang, Shuguang A-3004 Johnson, Christopher M. PS-6 L... more ... Goodacre, S. A-3003 Harrison, CA E-3000 Huang, Shuguang A-3004 Johnson, Christopher M. PS-6 Low, Jonathan A-3004 Mortley, D. E-3000 Nair, Sandhya A-3000 Omueti, Katherine O. PS-6 Powell, E. E-3000 Sato, J. Denry A-3001 Scoffield, J. E-3000 Sevak, Shruti A-3002 ...
The Louisiana Dept. of Agriculture and Forestry (LDAF) conducts sweetpotato weevil [SPW, Cylas fo... more The Louisiana Dept. of Agriculture and Forestry (LDAF) conducts sweetpotato weevil [SPW, Cylas formicarius ( Fabricius )] monitoring in support of the statewide SPW quarantine program. The monitoring activity primarily involves a statewide pheromone-based trapping process that generates trap data for sweetpotato beds and production fi elds. We conducted GIS analysis of SPW trap data, collected over three years, to assess the potential use of GIS tools in managing and interpreting the data. The LDAF has already generated shapefi les for all beds and fi elds in each of three years, facilitating GIS analysis. However, trap data was manually collected and statewide data was compiled and stored in spreadsheet fi les. Trap data was mapped to specifi c beds and fi elds in each of three years, generating layers that clearly showed fi elds and parishes that reported high trap counts. GIS analysis showed potential SPW "hotspots" in each year, indicating that certain beds or fi elds are more prone to SPW infestation than others. This information can be useful in planning SPW management strategies by growers and other stakeholders. The GIS database also provides the foundation for the development of descriptive and predictive models of SPW occurence in Louisiana. Compiling the SPW trap data into a GIS database allows the data to be distributed over the Internet, facilitating real-time access by stakeholders.
The United States of America collection of mountain mint (Pycnanthemum Michx.) is held at the USD... more The United States of America collection of mountain mint (Pycnanthemum Michx.) is held at the USDA-ARS National Clonal Germplasm Repository (NCGR) in Corvallis, Oregon as seed, potted plants and tissue cultures and a long-term storage collection is preserved at the USDA-ARS National Center for Genetic Resources Preservation (NCGRP) in Fort Collins, Colorado. The clonal collection is comprised of 34 accessions as potted plants that are duplicated with 31 accessions stored as in vitro cultures at 4 degrees C in tissue culture bags for medium-term storage at NCGR and as cryopreserved shoot tips in liquid nitrogen at NCGRP for long-term storage. This study reports on these two models of preservation of mountain mint at the U.S. National Plant Germplasm System. In vitro plants required 2 to 7 months for propagation on MS medium without growth regulators before storage at 4 degrees C. Plants remained in storage with good vigour in bags on 1/2x nitrogen MS medium without growth regulators for a mean of 2.08 y. An encapsulation-dehydration protocol was successful for cryopreservation of shoot tips from cold acclimated in vitro plants. Post-cryo viability, indicated by shoot tips with developed leaves and roots, ranged from 60 to 100 % for 27 accessions and 40 to 50 % for the other four. The encapsulation-dehydration cryopreservation method proved suitable for long-term preservation of the 31 Pycnanthemum accessions. These alternative storage forms allow for active use of the collection as well as base storage for clonally propagated accessions.
All rights reserved. No part of this periodical may be reproduced or transmitted in any form or b... more All rights reserved. No part of this periodical may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Permission for printing and for reprinting the material contained herein has been obtained by the publisher.
The genus Limnanthes (Limnanthaceae), also known as meadowfoam, has attracted attention for indus... more The genus Limnanthes (Limnanthaceae), also known as meadowfoam, has attracted attention for industrial use due to the unique characteristics of its seed oil. Samples from wild populations showed variability in agronomically important traits involved in seed oil yield, warranting the establishment and continued development of a germplasm collection. The level of genetic diversity within the United States Department of Agriculture, Agriculture Research Service, National Plant Germplasm System (USDA-ARS-NPGS) Limnanthes collection was evaluated using 15 simple sequence repeat (SSR) markers across 62 accessions representing 7 species. Parsimony analysis separated the accessions into two main groups consistent with the traditional taxonomic sections Inflexae and Reflexae, but there was little resolution within groups. These two groups were confirmed using neighbour-joining analysis and principal coordinate analysis. SSR marker variation suggests that the Limnanthes germplasm collection is genetically diverse and the accessions within the species likely contain novel alleles, and therefore the collection contributes to the conservation of the wild Limnanthes gene pool.
Secretion of phenolic compounds is a major limitation for sugarcane in vitro shoot culture, causi... more Secretion of phenolic compounds is a major limitation for sugarcane in vitro shoot culture, causing a loss of regenerative capacity and subsequent cell death. In this study, micropropagation and phenolic secretion of four Saccharum genotypes were evaluated in presence of different antioxidants. Aseptic cultures of S. officinarum (PI 184794 and PI 88652), S. sinense (PI 29109) and S. robustum (UNK R65P35) were propagated on medium containing antioxidants, citric acid (100 mg/L), L-cysteine (100 mg/L), polyvynylpirrolidone (300 mg/L) and L-glutathione (50 mg/L) in two consecutive subculture cycles. Interaction between genotypes and antioxidants was significant in both cycles. All genotypes showed good shoot formation, shoot vigor and color, except in PI 88652 which had less shoot development in both the presence and absence of the antioxidants tested. PI 184794 displayed the highest shoot proliferation in the presence of citric acid, and UNK R65P35 produced more shoots per explant in the 2 nd subculture. For S. sinense (PI 29109), in both subcultures, most shoots were observed in the presence of polyvynylpirrolidone. Medium discoloration due to phenolic secretion was reduced in the presence of citric acid and polyvynylpirrolidone. The type of secreted phenolic compounds differed with genotype as the Principal Component Analysis of cultivation media separated PI 88652 from PI 29109 and UKN R65P35. Phenolic compounds varied in composition and were secreted at various levels as a function of genotype and antioxidant type. Loadings plots indicated the genotype and antioxidant separations were broadly driven by flavonoid compounds.
Journal of the American Society for Horticultural Science, Jul 1, 2005
Garlic (Allium sativum L.) has been cultivated by asexual propagation since time immemorial. The ... more Garlic (Allium sativum L.) has been cultivated by asexual propagation since time immemorial. The discovery of male-fertile garlic accessions has opened a venue for genetic studies and improvement through sexual recombination. An S 1 family of 84 plants was generated from a single male-fertile heterozygous plant from the U.S. Dept. of Agriculture Plant Introduction 540316 and used to identify the fi rst genetic linkages in garlic based on single nucleotide polymorphisms, simple sequence repeats, and randomly amplifi ed polymorphic DNAs. Thirty-seven markers formed nine linkage groups covering 415 centimorgans (cM) with average distance of 15 cM between loci; other 16 loci remained unlinked. A male fertility locus was placed on the map. This fi rst genetic map of garlic is a seminal step toward the genetic improvement of garlic and eventual marker-assisted breeding. Garlic is an asexually propagated plant cultivated since time immemorial (Simon, 2004). The center of origin for garlic is central Asia, from which the plant spread worldwide and is now cultivated from mountainous tropical to temperate regions (Etoh and Simon, 2002). The bulb is the most economically important part of the garlic plant; however, its fresh leaves, pseudostems, and bulbils are also consumed by humans (Fritsch and Friesen, 2002). Although garlic is primarily consumed for its fl avor, it has well-defi ned medicinal benefi ts (Augusti, 1990; Block et al., 1984; Makheja and Bailey, 1990). The organosulfur compounds accumulated by garlic show signifi cant antibacterial, antifungal, anticarcinogenic, antiasthmatic, and antidiabetic properties, as well as lipid-lowering ability through inhibition of cholesterol biosynthesis (Keusgen, 2002). Commercial production of garlic is exclusively by asexual propagation. Sterility in garlic has been attributed to both genetic and environmental factors, including irregular chromosome pairing, chromosomal deletion, anther degeneration, and competition for nutrients between topsets and fl owers (Etoh and Simon, 2002). Because of asexual propagation, garlic improvement has been limited to clonal selection of spontaneous mutants or sports
Bulgaria is famous for its 330-year-old-tradition in rose oil production, which is based on the K... more Bulgaria is famous for its 330-year-old-tradition in rose oil production, which is based on the Kazanluk rose (Rosa damascena Mill. f. trigintipetala Dieck.). The Bulgarian rose oil (otto) is recognized as the ultimate rose oil. For successful selection and breeding work of oil-bearing roses, information is needed on the variation of morphological and phenological characteristics and essential oil composition of locally available genotypes. We estimated the correlation coefficients between yields and morphological characteristics of 15 genotypes of Bulgarian oil-bearing rose. It was found that rose yields depended mostly on the number of flowers, the number of flower branches per bush, and the weight of individual flowers (r = 0.99, 0.88, and 0.84, respectively). Also, we established correlations between the concentrations of various essential oil constituents of the Bulgarian rose oil. Generally, higher concentration of citronellol + nerol was associated with lower concentration of geraniol and stereo-terpens (r = -0.76 and -0.59, respectively). Also, higher concentration of citronellol + nerol was positively correlated to increased concentration of terpene aldehydes (r = 0.63) and esters (r = 0.48). The geraniol concentration was positively correlated to stearoptenes (r = 0.57). Both morphological characteristics and essential oil constituents should be used for further selection of high-yielding cultivars with desirable essential oil composition.
Its fl owers are predominantly allogamous; however, self-compatible plants may be found in the wi... more Its fl owers are predominantly allogamous; however, self-compatible plants may be found in the wild. Plants of this genus typically occupy riparian habitats and grow well in temperatures between 4 and 16 °C. Meadowfoam may therefore be considered as a winter-spring crop; however, extended periods of air temperatures below 5 °C are detrimental . Early agronomic studies indicated Limnanthes as suitable to be grown in California, Maryland, Oregon, and Alaska (Higgins et al., 1971).
Until recently, there has been no large-scale production of true seeds in garlic (A. sativum L. a... more Until recently, there has been no large-scale production of true seeds in garlic (A. sativum L. and A. longicuspis L.). The recent discovery of male fertile garlic accessions stimulated research on the genetics and breeding of garlic. However, there is no information regarding the phenotypic characteristics of garlic populations generated from true seeds. We evaluated the fi rst generation of sexually derived families of garlic for bulb and clove weight, number of cloves per bulb, fl ower stalk height, number of leaves, plant height, and days required to achieve bulb maturity. Signifi cant variations were observed within and among families for these important traits.
Enormous genomic resources have been developed for plants in the monocot order Poales; however, i... more Enormous genomic resources have been developed for plants in the monocot order Poales; however, it is not known how useful these resources will be for other economically important monocots. Asparagales are a monophyletic order sister to class Commelinanae that carries Poales, and is the second most economically important monocot order. Development of genomic resources for and their application to Asparagales are challenging because of huge nuclear genomes and the relatively long generation times required to develop segregating families. We synthesized a normalized eDNA library of onion (Allium cepa) and produced II ,008 unique expressed sequence tags (ESTs) for comparative genomic analyses of Asparagales and Poales. Alignments of onion ESTs, Poales ESTs, and genomic sequences from rice were used to design oligonucleotide primers amplifying genomic regions from asparagus, garlic, and onion. Sequence analyses of these genomic regions revealed microsatellites, insertions/deletions, and single nucleotide polymorphisms for comparative mapping of rice and Asparagales vegetables. Initial mapping revealed no obvious synteny at the recombinationallevel between onion and rice, indicating that genomic resources developed for Poales may not be applicable to the monocots as a whole. Genomic analyses of Asparagales would greatly benefit from EST sequencing and deep-coverage, large-insert genomic libraries of representative small-genome model species within the "higher" and "lower" Asparagales, such as asparagus and orchid, respectively.
Germanium has been reported as a mineral element affecting plant cell metabolism. Many trials to ... more Germanium has been reported as a mineral element affecting plant cell metabolism. Many trials to supply germanium to fruit have been carried out since tests have confi rmed germanium's role as a medical substance. Supplying germanium to orchards by soil and foliar application was not effective because of loss from rainfall. Also, tree injection with germanium solution required the insertion of a tube to the tree xylem at each injection site. In order to increase germanium absorption by fruit, this study carried out the postharvest dipping of fruit into germanium solution. 'Niitaka' pear ( Pyrus pyrifolia ) fruit was treated with two types of germanium, GeO (inorganic type) and Ge-132 (organic type), in a concentration of 50 mg•L -1 just after harvest in early Oct. 2004. Flesh browning after peeling the fruit was delayed by germanium treatment, and polyphenoloxidase (PPO) activities were lowered. Postharvest potentials were maintained at high levels for fruit fi rmness, physiological disorders, and decayed fruit during cold storage at 0 to 1 °C for 2 months. Antioxidant and some phenolic compounds were higher than those of control fruit.
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
The Louisiana Dept. of Agriculture and Forestry (LDAF) conducts sweetpotato weevil [SPW, Cylas fo... more The Louisiana Dept. of Agriculture and Forestry (LDAF) conducts sweetpotato weevil [SPW, Cylas formicarius (Fabricius)] monitoring in support of the statewide SPW quarantine program. The monitoring activity primarily involves a statewide pheromone-based trapping process that generates trap data for sweetpotato beds and production fi elds. We conducted GIS analysis of SPW trap data, collected over three years, to assess the potential use of GIS tools in managing and interpreting the data. The LDAF has already generated shapefi les for all beds and fi elds in each of three years, facilitating GIS analysis. However, trap data was manually collected and statewide data was compiled and stored in spreadsheet fi les. Trap data was mapped to specifi c beds and fi elds in each of three years, generating layers that clearly showed fi elds and parishes that reported high trap counts. GIS analysis showed potential SPW "hotspots" in each year, indicating that certain beds or fi elds are more prone to SPW infestation than others. This information can be useful in planning SPW management strategies by growers and other stakeholders. The GIS database also provides the foundation for the development of descriptive and predictive models of SPW occurence in Louisiana. Compiling the SPW trap data into a GIS database allows the data to be distributed over the Internet, facilitating real-time access by stakeholders. 2 Poster withdrawn.
In Vitro Cellular & Developmental Biology - Animal, 2007
... Goodacre, S. A-3003 Harrison, CA E-3000 Huang, Shuguang A-3004 Johnson, Christopher M. PS-6 L... more ... Goodacre, S. A-3003 Harrison, CA E-3000 Huang, Shuguang A-3004 Johnson, Christopher M. PS-6 Low, Jonathan A-3004 Mortley, D. E-3000 Nair, Sandhya A-3000 Omueti, Katherine O. PS-6 Powell, E. E-3000 Sato, J. Denry A-3001 Scoffield, J. E-3000 Sevak, Shruti A-3002 ...
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