Así como entramos en un nuevo siglo, el siglo de los "cambios ", el concepto de Calidad ha ido su... more Así como entramos en un nuevo siglo, el siglo de los "cambios ", el concepto de Calidad ha ido sufriendo modificaciones desde su inicial enfoque de "bueno o malo", a un concepto de satisfacción de las necesidades del cliente al mejor costo posible. Es decir se incluyen dos pilares básicos que son: Cliente y Costos. Desde hace muchos años se han desarrollado en Occidente diferentes enfoques tendientes a poder
... un detector UV-visible L-2420, bomba L-2130, muestreador automático L-2200,organizador y prog... more ... un detector UV-visible L-2420, bomba L-2130, muestreador automático L-2200,organizador y programa computacional del proveedor según las instrucciones del fabricante, como se describe en el manual del usuario del equipo. ...
Biochimica Et Biophysica Acta - Proteins And Proteomics, Nov 1, 1993
The local motion of protoporphyrin IX in the heme pocket of horseradish peroxidase has been studi... more The local motion of protoporphyrin IX in the heme pocket of horseradish peroxidase has been studied using fluorescence methods. The temperature dependence of the anisotropy and lifetime of a protoporphyrin IX-apo-horseradish peroxidase complex, dissolved in a solution of 80% glycerol and 20% buffer (0.1 M phosphate, pH 7.4), was determined. Anisotropy data were analyzed in terms of the thermal coefficient of the frictional resistance to fluorophore movement. The resultant 'Y' plot was characterized by three distinct slopes. The slope corresponding to the lowest temperature regime agreed with the value obtained for fluorophores not complexed with protein. The slope corresponding to an intermediate temperature was lower indicating a larger resistance to porphyrin rotation. At higher temperatures this resistance to rotation diminished as evidenced by the increased slope. These results are contrasted with those obtained with the protoporphyrin IX-apomyoglobin complex.
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1993
The local motion of protoporphyrin IX in the heme pocket of horseradish peroxidase has been studi... more The local motion of protoporphyrin IX in the heme pocket of horseradish peroxidase has been studied using fluorescence methods. The temperature dependence of the anisotropy and lifetime of a protoporphyrin IX-apo-horseradish peroxidase complex, dissolved in a solution of 80% glycerol and 20% buffer (0.1 M phosphate, pH 7.4), was determined. Anisotropy data were analyzed in terms of the thermal coefficient of the frictional resistance to fluorophore movement. The resultant 'Y' plot was characterized by three distinct slopes. The slope corresponding to the lowest temperature regime agreed with the value obtained for fluorophores not complexed with protein. The slope corresponding to an intermediate temperature was lower indicating a larger resistance to porphyrin rotation. At higher temperatures this resistance to rotation diminished as evidenced by the increased slope. These results are contrasted with those obtained with the protoporphyrin IX-apomyoglobin complex.
Así como entramos en un nuevo siglo, el siglo de los "cambios ", el concepto de Calidad ha ido su... more Así como entramos en un nuevo siglo, el siglo de los "cambios ", el concepto de Calidad ha ido sufriendo modificaciones desde su inicial enfoque de "bueno o malo", a un concepto de satisfacción de las necesidades del cliente al mejor costo posible. Es decir se incluyen dos pilares básicos que son: Cliente y Costos. Desde hace muchos años se han desarrollado en Occidente diferentes enfoques tendientes a poder
... un detector UV-visible L-2420, bomba L-2130, muestreador automático L-2200,organizador y prog... more ... un detector UV-visible L-2420, bomba L-2130, muestreador automático L-2200,organizador y programa computacional del proveedor según las instrucciones del fabricante, como se describe en el manual del usuario del equipo. ...
Biochimica Et Biophysica Acta - Proteins And Proteomics, Nov 1, 1993
The local motion of protoporphyrin IX in the heme pocket of horseradish peroxidase has been studi... more The local motion of protoporphyrin IX in the heme pocket of horseradish peroxidase has been studied using fluorescence methods. The temperature dependence of the anisotropy and lifetime of a protoporphyrin IX-apo-horseradish peroxidase complex, dissolved in a solution of 80% glycerol and 20% buffer (0.1 M phosphate, pH 7.4), was determined. Anisotropy data were analyzed in terms of the thermal coefficient of the frictional resistance to fluorophore movement. The resultant 'Y' plot was characterized by three distinct slopes. The slope corresponding to the lowest temperature regime agreed with the value obtained for fluorophores not complexed with protein. The slope corresponding to an intermediate temperature was lower indicating a larger resistance to porphyrin rotation. At higher temperatures this resistance to rotation diminished as evidenced by the increased slope. These results are contrasted with those obtained with the protoporphyrin IX-apomyoglobin complex.
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1993
The local motion of protoporphyrin IX in the heme pocket of horseradish peroxidase has been studi... more The local motion of protoporphyrin IX in the heme pocket of horseradish peroxidase has been studied using fluorescence methods. The temperature dependence of the anisotropy and lifetime of a protoporphyrin IX-apo-horseradish peroxidase complex, dissolved in a solution of 80% glycerol and 20% buffer (0.1 M phosphate, pH 7.4), was determined. Anisotropy data were analyzed in terms of the thermal coefficient of the frictional resistance to fluorophore movement. The resultant 'Y' plot was characterized by three distinct slopes. The slope corresponding to the lowest temperature regime agreed with the value obtained for fluorophores not complexed with protein. The slope corresponding to an intermediate temperature was lower indicating a larger resistance to porphyrin rotation. At higher temperatures this resistance to rotation diminished as evidenced by the increased slope. These results are contrasted with those obtained with the protoporphyrin IX-apomyoglobin complex.
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